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Volume 22 Issue 5, May 2019

Multiplexed EM labeling

Characterizing synaptic connectivity between neurons using electron microscopy is important in drawing the ‘wiring diagram’ of the nervous system. Zhang and colleagues developed a peroxidase-based multiplexed electron microscopy labeling technique that enables simultaneous visualization of multiple cell types without the need for spectral separation, facilitating the investigation of synaptic connectivity between genetically defined neuronal populations. The cover image shows an ultrathin section from the spinal cord dorsal horn with three neuronal populations simultaneously labeled: local inhibitory interneurons (red, endoplasmic reticulum labeled), corticospinal inputs (green, cytoplasm labeled) and somatosensory primary afferents (blue, mitochondrial matrix labeled).

See Zhang et al.

Image credit: Qiyu Zhang. Cover design: Marina Corral Spence.

News & Views

  • A new study reveals an unexpected mechanism underlying behavioral abnormalities in the neurodevelopmental disorder Williams syndrome. A deficit in myelination, resulting from the deletion of a Williams syndrome-associated gene in forebrain excitatory neurons, causes hypersociability by impairing action potential conduction. Accordingly, rescuing myelination or conduction normalizes this behavior.

    • Lindsay A. Osso
    • Jonah R. Chan
    News & Views


  • DNA damage or cellular stresses can induce senescence, and increased senescence with aging contributes to age-associated tissue damage, inflammation and disease. Zheng and colleagues report increased senescent oligodendrocyte progenitor cells around amyloid plaques. Therapeutically eliminating these senescent cells may influence the onset and progression of Alzheimer’s disease pathology.

    • David Holtzman
    • Jason Ulrich
    News & Views
  • The ventrolateral and medial orbitofrontal cortices are involved in selecting actions based on the value of expected outcomes. Malvaez and colleagues reveal that these brain regions are specialized in value encoding (ventrolateral orbitofrontal cortices) versus value memory retrieval (medial orbitofrontal cortices) and that they interact with the basolateral amygdala to orchestrate goal-oriented reward-seeking.

    • Shannon L. Gourley
    News & Views
  • Noninvasive delivery of alternating electrical currents to temporal and prefrontal brain regions improves working memory and reverses age-related changes in brain dynamics in the elderly, report Reinhart and Nguyen in this issue of Nature Neuroscience. They also report a similar effect in young adults with poor working memory performance.

    • Romain Quentin
    • Leonardo G. Cohen
    News & Views
  • Robust conclusions require rigorous statistics. In 2009 a seminal paper described the dangers and prevalence of double-dipping in neuroscience. Ten years on, I consider progress toward statistical rigor in neuroimaging.

    • Katherine S. Button
    News & Views
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