Reconstruction of nerve bundles in a rhesus monkey brain. Fang Xu et al. present a method to image a whole monkey brain at micrometer resolution in 100 hours.

See Xu et al.

Nguyen et al. developed a lyophilized synthetic biology circuit embedded in fabric that transmits viral detection signals through a textile-integrated waveguide.

See Nguyen et al.

Choi et al. engineer a biodegradable pacemaker without external leads that improves the safety of temporary cardiac pacing.

See Y. S. Choi et al.

SEQC2, the final phase of the MAQC consortium, presents the results of several sequencing benchmarking analyses.

SeeEditorial

Schede et al. applied a new tomographic spatial transcriptomic method, STRP-seq—which is able to provide tissue-wide gene expression maps for thousands of genes—to the brain of the bearded dragon (Pogona vitticeps).

See Schede et al.

Decoding epigenomic information from individual cells from the mouse brain: with single-cell CUT&Tag, histone modifications and transcription factor binding can now be mapped in tens of thousands of individual cells.

See Bartosovic et al. and Wu et al.

Scanning electron micrograph of a heart-forming organoid showing a myocardial layer (pink) separated by a thin endocardial layer (blue) from an anterior foregut endoderm and vascular layer (cinnamon); the outer layer (yellow) includes liver anlagen (red). The layers were hand colored on the basis of cell shape, cell surface and data from light and confocal microscopy.

See Drakhlis et al.

An artist's impression of what we can learn from assaying chromatin fragments released from dying cells; the whole body is reflected in a blood sample. Sadeh et al. present a method for assaying these fragments to study cell type and state in health and disease.

See Sadeh et al.

Illustration showing the completion of a genomic catalog of Earth’s microbiomes. Nayfach et al. apply metagenomics to more than ten thousand samples from diverse habitats, extending the known phylogenetic diversity of bacteria and archaea.

See Nayfach et al.

Nature Biotechnology celebrates 25 years of publishing the very best of biotech science and business.

See Editorial

Colorized scanning electron micrograph of silicone-encapsulated gold interconnects of a device for epineural optogenetic neuromodulation. Michoud et al. use the device to show how nociceptor activation drives protective pain behavior and inflammation.

See Michoud et al.

Colored scanning electron micrograph of resting human T lymphocytes. Mo et al. present an alloimmune defense receptor that allows allogeneic T cells to resist rejection by the host immune system by deleting activated host T and NK cells.

See Mo et al.