Volume 37 Issue 8, August 2019

Potent siRNA silencing in the brain

High-resolution image of the primate dentate gyrus, a structure in the hippocampus, showing neurons (green), glia (purple), cell nuclei (blue) and divalent siRNAs (Di-siRNA, red). Alterman et al. demonstrate that Di-siRNAs distribute broadly in the brains of mice and non-human primates and enable potent and persistent gene silencing.

See Alterman et al.

Image: Chantal Ferguson. Cover Design: Erin Dewalt.

Editorial

Climate of fear

Heavy-handed targeting of ethnic Chinese researchers, students and investors by US funding, intelligence and immigration agencies poses a threat to the American life-sciences sector.

Editorial 02 Aug 2019

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News

Pancreatic cancer provides testbed for first mechanotherapeutics

Pancreatic cancer treatments are forging the path for a new line of agents that target the physical properties of a tumor and its surrounding microenvironment.

Cormac Sheridan
News 12 Jul 2019
Around the world in a month

News in Brief 02 Aug 2019
Portable CRISPR-based diagnostics

News in Brief 02 Aug 2019
Microsoft makes splash in AI-enabled lab solutions

Microsoft unveils the first collaborations for its Station B, a platform to automate lab experiments so scientists can test and reproduce ever-more-complex designs.

Eric Smalley
News 07 Jul 2019
Pfizer lured by Array’s cancer know-how

News in Brief 02 Aug 2019
Click chemistry targets antibody-drug conjugates for the clinic

Bioorthogonal chemistry, already a workhorse of drug discovery research, prepares for the leap into human testing.

Mark Peplow

Collection:

Nobel Prize in Chemistry 2022
News 04 Jul 2019
First Rounders Podcast
- First Rounders: Cigall Kadoch
  
  First Rounders Podcast 02 Aug 2019
Data Page
- 2Q19—Biotech funding stagnates
  
  Laura DeFrancesco
  Data Page 02 Aug 2019
- Drug pipeline: 2Q19
  
  Laura DeFrancesco
  Data Page 02 Aug 2019
News Feature
- Your DNA broker
  
  Millions of people each year are giving up their genotype information for research, for health, for fun and now for profit.
  
  Laura DeFrancesco
  Ariel Klevecz
  News Feature 16 Jul 2019

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Correspondence

Leveraging crowdsourcing to accelerate global health solutions

Sage Davis
Katrina Button-Simons
Geoffrey H. Siwo
Correspondence 19 Jul 2019
Clarify the HIPAA right of access to individuals’ research data

Christi J. Guerrini
Jeffrey R. Botkin
Amy L. McGuire
Correspondence 23 Jul 2019
Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2

Evan Bolyen
Jai Ram Rideout
J. Gregory Caporaso
Correspondence 24 Jul 2019

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Comment

It takes a planet

To take full advantage of deep-learning solutions in healthcare, the United States and China should collaborate, not compete.

Eric Topol
Kai-Fu Lee
Comment 02 Aug 2019

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Features

Patents
- How logically impossible patents block biosimilars
  
  Deep patent portfolios help drugmakers block market entry by potential biosimilars, but many biologic patents logically cannot be valid barriers to competitive entry.
  
  W. Nicholson Price II
  Arti K. Rai
  Patents 02 Aug 2019
- Xenotransplantation
  
  Recent patents related to xenografts, tissue repair and prevention of xenotransplant rejection.
  
  Patents 02 Aug 2019

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News & Views

Doubling down on siRNAs in the brain

Divalent siRNAs induce widespread gene silencing in the brains of mice and non-human primates.

Beverly L. Davidson
News & Views 02 Aug 2019
Goodbye reference, hello genome graphs

Algorithms for building a variant-aware human graph genome enable fast and accurate genotyping without huge memory requirements.

Adam Ameur
News & Views 02 Aug 2019

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Research

Perspectives
- Overcoming genetic heterogeneity in industrial fermentations
  
  Detection and mitigation of strain instability in industrial microbiology scale-up.
  
  Peter Rugbjerg
  Morten O. A. Sommer
  Perspective 08 Jul 2019
Letters
- Spatial metagenomic characterization of microbial biogeography in the gut
  
  The spatial distribution of bacterial species in the mouse gut is measured by microscopic ‘plot sampling’.
  
  Ravi U. Sheth
  Mingqiang Li
  Harris H. Wang
  Letter 22 Jul 2019
Articles
- A divalent siRNA chemical scaffold for potent and sustained modulation of gene expression throughout the central nervous system
  
  A divalent siRNA architecture enables sustained silencing of gene expression in deep regions of the brain.
  
  Julia F. Alterman
  Bruno M. D. C. Godinho
  Anastasia Khvorova
  
  Collection:
  
  25th Anniversary Collection
  Article 02 Aug 2019
- Epicardial cells derived from human embryonic stem cells augment cardiomyocyte-driven heart regeneration
  
  Cell therapy in a rat model of heart attack is enhanced by stem-cell-derived epicardium.
  
  Johannes Bargehr
  Lay Ping Ong
  Sanjay Sinha
  
  Collection:
  
  Stem cells from development to the clinic
  Article 02 Aug 2019
- Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype
  
  A graph-based genome indexing scheme enables variant-aware alignment of sequences with very low memory requirements.
  
  Daehwan Kim
  Joseph M. Paggi
  Steven L. Salzberg
  Article 02 Aug 2019
- Droplet-based combinatorial indexing for massive-scale single-cell chromatin accessibility
  
  Combining microfluidics with combinatorial indexing enables rapid mapping of genome accessibility in hundreds of thousands of single cells.
  
  Caleb A. Lareau
  Fabiana M. Duarte
  Jason D. Buenrostro
  Article 24 Jun 2019
- Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustion
  
  A droplet-based single-cell ATAC-seq method enables rapid mapping of chromatin accessibility in tens of thousands of cells.
  
  Ansuman T. Satpathy
  Jeffrey M. Granja
  Howard Y. Chang
  Article 02 Aug 2019
- Hybrid metagenomic assembly enables high-resolution analysis of resistance determinants and mobile elements in human microbiomes
  
  A more accurate metagenomics approach uses a hybrid assembler to incorporate short- and long-read sequences.
  
  Denis Bertrand
  Jim Shaw
  Niranjan Nagarajan
  Article 29 Jul 2019
- Enhancing gene editing specificity by attenuating DNA cleavage kinetics
  
  Engineered zinc finger nucleases achieve 98% knockout of a target gene in human T cells without detectable off-target activity.
  
  Jeffrey C. Miller
  Deepak P. Patil
  Edward J. Rebar
  Article 29 Jul 2019
Resources
- Compendium of 4,941 rumen metagenome-assembled genomes for rumen microbiome biology and enzyme discovery
  
  Comprehensive resource of cattle rumen genomes and a database of predicted proteins.
  
  Robert D. Stewart
  Marc D. Auffret
  Mick Watson
  Resource Open Access 02 Aug 2019

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Amendments & Corrections

Author Correction: A systems biology pipeline identifies regulatory networks for stem cell engineering

Melissa A. Kinney
Linda T. Vo
George Q. Daley
Author Correction 16 Jul 2019
Publisher Correction: Reply to ‘A lack of evidence for six times more anxiety and depression in US graduate students than in the general population’

Teresa M. Evans
Lindsay Bira
Nathan L. Vanderford
Publisher Correction 09 Jul 2019
Publisher Correction: Drug pipeline 1Q19

Laura DeFrancesco
Publisher Correction 22 Jul 2019

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Careers & Recruitment

Career Feature
- Second-quarter biotech job picture
  
  A quarterly snapshot of job expansions, reductions and availability in the biotech and pharma sectors.
  
  Michael Francisco
  Career Feature 02 Aug 2019
People
- People
  
  A sample of goings-on in and around the biotech and pharmaceutical industry.
  
  People 02 Aug 2019

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Volume 37 Issue 8, August 2019

Potent siRNA silencing in the brain

Editorial

News

First Rounders Podcast

Data Page

News Feature

Correspondence

Comment

Features

Patents

News & Views

Research

Perspectives

Letters

Articles

Resources

Amendments & Corrections

Careers & Recruitment

Career Feature

People

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