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Light-Inducible Expression of a Chimeric Gene in Soybean Tissue Transformed with Agrobacterium

Bio/Technology volume 3, pages 241–246 (1985)Cite this article

Abstract

Soybean (Glycine max cv. “Forrest”) has been successfully transformed with Agrobacterium containing a bacterial kanamycin resistance gene linked to the 5′ portion of a soybean small subunit carboxylase gene. Comparison of light-grown versus dark-grown transformed callus demonstrated light induction of the chimeric gene as measured by increased levels of kanamycin resistance. This is not unexpected as small subunit genes are under light regulation.

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Authors and Affiliations

  1. Calgene, Inc., 1920 Fifth Street, Davis, CA, 95616

    D. Facciotti, J. K. O'Neal, S. Lee & C. K. Shewmaker

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  2. J. K. O'Neal
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Facciotti, D., O'Neal, J., Lee, S. et al. Light-Inducible Expression of a Chimeric Gene in Soybean Tissue Transformed with Agrobacterium. Nat Biotechnol 3, 241–246 (1985). https://doi.org/10.1038/nbt0385-241

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