Abstract
Cell culture of Taxus spp. represents a potential alternative source of taxol and related taxanes used in cancer chemotherapy. We have analyzed the effect of different culture media components on growth and production of taxol in developing callus cultures of T. cuspidata. Several sequential modifications were made to the basal B5 medium, which included addition and/or variation in the concentration of sucrose, B5 organic supplements, gibberellic acid, 36 combinations of 2,4-D/kinetin ratios, media salts and organic supplements, phenylalanine, casein hydrolysate and medium pH. The experiments were conducted during a 55 day-growth period followed by taxane extraction and analysis. Significant increases in taxol yield and growth over basal medium grown calli were observed with some of the modified media.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Slichenmyer, W.J. and Von Hoff, D.D. 1991. Taxol: a new and effective anti-cancer drug. Anti-Cancer Drugs 2: 519–530.
Fett-Neto, A.G., DiCosmo, F., Reynolds, W.F. and Sakata, K., 1992. Cell culture of Taxus as a source of the antineoplastic drug taxol and related taxanes. Bio/Technology 10: 1572–1575.
Fett-Neto, A.G. and DiCosmo, F. 1992. Distribution and amounts of taxol in different shoot parts of Taxus cuspidata. Planta Medica 58: 464–466.
Savidge, R.A. 1990. Phytohormonal regulation of cambial growth in trees, p. 142–151. In: Fast Growing Trees and Nitrogen Fixing Trees. Wemer, D. and Müller, P. (Eds. ). Gustav Fisher Verlag, Sttutgart, New York.
Leete, E. and Bodem, G.B. 1966. The biosynthesis of 3-dimethylamino-3-phenylpropanoic acid in yew. Tetrahedron Letters 33: 3925–3927.
Becker, H. and Sauerwein, M. 1990. Manipulating the biosynthetic capacity of plant cell cultures, p. 43–57. In: Secondary Products from Plant Tissue Culture. Charlwood, B. V. and Rhodes, M. J. C. (Eds.). Caledon Press, Oxford, UK.
Marschner, H. 1986. Mineral Nutrition of Higher Plants. Academic Press, London, UK.
Schenk, R.U. and Hildebrandt, A.C. 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204.
Delfel, N.E. and Rothfus, J.A. 1977. Antitumour alkaloids in callus cultures of Cephalotaxus harringtonia. Phytochemistry 16: 1595–1598.
Brown, J.T. and Charlwood, B.V. 1990. Organogenesis in callus culture, p. 65–70. In: Methods in Molecular Biology, Vol. 6, Plant cell and Tissue Culture. Pollard, J. W. and Walker, J. M. (Eds.). Humana Press, Clifton, NJ.
Charlwood, B.V., Charlwood, K.A. and Molina-Torres, J. 1990. Accumulation of secondary compounds by organized plant cultures, p. 167–200. In: Secondary Products from Plant Tissue Culture. Charlwood, B. V. and Rhodes, M. J. C. (Eds. ). Caledon Press, Oxford.
Gamborg, O.L., Miller, R.A. and Ojima, K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Expt. Cell Res. 50: 151–158.
Whiterup, K.M., Look, S.A., Stasko, M.W., Guiorzi, T.J., Muschik, G.M. and Cragg, G.M. 1990. Taxus spp. needles contain amounts of taxol comparable to the bark of Taxus brevifolia: analysis and isolation. J. Nat. Prod. 53: 1249–1255.
Snedecor, G.W. and Cochran, W.G. 1982. Statistical Methods. The Iowa State University Press, Ames, IA.
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fett-Neto, A., Melanson, S., Sakata, K. et al. Improved Growth and Taxol Yield in Developing Calli of Taxus cuspidata by Medium Composition Modification. Nat Biotechnol 11, 731–734 (1993). https://doi.org/10.1038/nbt0693-731
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/nbt0693-731
This article is cited by
-
Enhanced productivity of atropine in cell suspension culture of Hyoscyamus muticus L.
In Vitro Cellular & Developmental Biology - Plant (2022)
-
Embryo culture, callus induction, and flavonoid profile of Strychnos pseudoquina A. St.-Hil., an important medicinal species from the Brazilian Cerrado biome
Plant Cell, Tissue and Organ Culture (PCTOC) (2021)
-
Identification of rate-limiting enzymes involved in paclitaxel biosynthesis pathway affected by coronatine and methyl-β-cyclodextrin in Taxus baccata L. cell suspension cultures
DARU Journal of Pharmaceutical Sciences (2018)
-
Studies on the glyphosate-induced amino acid starvation and addition of precursors on caffeic acid accumulation and profiles in adventitious roots of Echinacea purpurea (L.) Moench
Plant Cell, Tissue and Organ Culture (PCTOC) (2015)
-
Transcriptome profiling shows gene regulation patterns in a flavonoid pathway in response to exogenous phenylalanine in Boesenbergia rotunda cell culture
BMC Genomics (2014)
