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An unbiased approach for the genome-wide detection of off-target cleavage by CRISPR-Cas9 RNA–guided nucleases reveals wide variability in the off-target activity of different guide RNAs.
Methylase-assisted bisulfite sequencing allows to determine the genomic locations of the cytosine demethylation intermediates 5-formylcytosine and 5-carboxylcytosine at base pair resolution.
Coupling limited proteolysis and a proteomics workflow enables measurement of both subtle and wholesale protein conformational changes in a eukaryotic proteome.
For intact RNA, gene expression profiles from rRNA-depletion and poly-A enrichment are similar. In addition, rRNA- depletion enables effective analysis of degraded RNA samples.
The Sequencing Quality Control (SEQC) consortium shows that junction discovery and differential gene expression profiling with RNA-seq can be robust but transcript-level and absolute measurements remain challenging.
A comparison of RNA-seq and microarray data from samples treated with diverse drugs highlights a dependency of cross-platform concordance on treatment effect.