Abstract
Although it is well documented that plant tissue responds to phytohormones by expressing new genes1,2, the signal/transduction pathway from hormone to gene is not well understood. DNA sequences regulating plant tissue responses to other external signals such as light2, heat3 and anaerobic stress4 have been characterized. Regulatory sequences involved in hormonal activation of genes have not been described from any plant, however. We report here the normal regulation of a promoter from wheat, inducible by the phytohormone abscisic acid (ABA), in a transient expression assay using rice protoplasts: an isolated plant promoter fragment is thus responding specifically to hormone treatment, and provides direct evidence for phytohormone acting to regulate the initiation of gene transcription. The response is rapid (within 60 min), which should allow identification of intermediates between the ABA signal and its regulation of gene expression.
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Marcotte, W., Bayley, C. & Quatrano, R. Regulation of a wheat promoter by abscisic acid in rice protoplasts. Nature 335, 454–457 (1988). https://doi.org/10.1038/335454a0
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DOI: https://doi.org/10.1038/335454a0
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