Abstract
INTRASPECIFIC and interspecific fusion of plant protoplasts is induced by agents1–4. Protoplasts of several species can regenerate a cell wall and divide to form callus, from which whole plants can be grown5. To combine these processes and obtain hybrid cells and then hybrid plants several requirements must be met6. The parental protoplasts should be capable of being cultured, as should the hybrid cells, and plant regeneration must be possible. Selection procedures developed so far7–10 may not be generally applicable, and we have now developed a method which makes use of naturally occurring differences in the sensitivity of cultured plant protoplasts to growth media and drugs11.
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POWER, J., FREARSON, E., HAYWARD, C. et al. Somatic hybridisation of Petunia hybrida and P. parodii. Nature 263, 500–502 (1976). https://doi.org/10.1038/263500a0
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DOI: https://doi.org/10.1038/263500a0
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