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Evidence for the Existence of a Minimum of Two Phases of Ordered Water in Skeletal Muscle

Abstract

NMR spectra of muscle water suggest that it exists in at least two ordered phases, which can be distinguished by the widths of their NMR signals, by deuterium exchange and by vacuum drying. Heat denaturation of muscle protein lessens this ordering.

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References

  1. Szent-Gyorgyi, A., Bioenergetics (Academic Press, New York, 1957).

    Google Scholar 

  2. Klotz, I. M., Science, 128, 815 (1958).

    Article  ADS  CAS  Google Scholar 

  3. Berendsen, H. J. C., J. Chem. Phys., 36, 3297 (1962).

    Article  ADS  CAS  Google Scholar 

  4. Beeman, W. W., Geil, P., Shurman, M., and Malmon, A. G., Acta Cryst., 10, 818 (1957).

    Google Scholar 

  5. Hearst, J. E., and Vinograd, J., Proc. US Nat. Acad. Sci., 47, 1005 (1961).

    Article  ADS  CAS  Google Scholar 

  6. Ritland, H. N., Kaesberg, P., and Beeman, W. W., J. Chem. Phys., 18, 1237 (1950).

    Article  ADS  CAS  Google Scholar 

  7. Blears, D. J., and Danyluk, S. S., Biochim. Biophys. Acta, 154, 17 (1968).

    Article  CAS  Google Scholar 

  8. Ling, G. N., J. Gen. Physiol., 43, 149 (1960).

    Article  CAS  Google Scholar 

  9. Ling, G. N., A Physical Theory of the Living State (Blaisdell, New York, 1962).

    Google Scholar 

  10. Ling, G. N., Ann. NY Acad. Sci., 125, 401 (1965).

    Article  ADS  CAS  Google Scholar 

  11. McLaughlin, S. G. H., and Hinke, J. A. M., Canad. J. Physiol. Pharmacol., 44, 837 (1966).

    Article  CAS  Google Scholar 

  12. Cope, F. W., J. Gen. Physiol., 50, 1353 (1967).

    Article  CAS  Google Scholar 

  13. Cope, F. W., Bull. Math. Biophysics, 29, 583 (1967).

    Article  CAS  Google Scholar 

  14. Chapman, G., and McLaughlin, K. A., Nature, 215, 391 (1967).

    Article  ADS  CAS  Google Scholar 

  15. Fritz, O. G., and Swift, T. J., Biophysical J., 5, 675 (1967).

    Article  Google Scholar 

  16. Hazlewood, C. F., and Nichols, B. L., Johns Hopkins Med. J., 123, 198 (1968).

    PubMed  CAS  Google Scholar 

  17. Kavanaugh, J. L., Water and Solute–Water Interactions, 101 (Holden–Day, Inc., San Francisco, 1964). Emsley, J. W., Feeney, J., and Sutcliffe, L. H., High Resolution Nuclear Magnetic Resonance Spectroscopy, 1 (Pergamon, New York, 1965).

    Google Scholar 

  18. Zierler, K. L., Amer. J. Physiol., 197, 515 (1959).

    CAS  Google Scholar 

  19. Hechter, O., Wittstruck, T., McNiven, N., and Lester, G., Proc. US Nat Acad. Sci., 46, 783 (1960).

    Article  ADS  CAS  Google Scholar 

Download references

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HAZLEWOOD, C., NICHOLS, B. & CHAMBERLAIN, N. Evidence for the Existence of a Minimum of Two Phases of Ordered Water in Skeletal Muscle. Nature 222, 747–750 (1969). https://doi.org/10.1038/222747a0

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