Volume 100 Issue 8, August 2020

Volume 100 Issue 8

The cover shows the effects of vasoinhibin in murine antigen-induced arthritis. For more information, see the paper by Ortiz et al, p 1068, this issue.

Inside the USCAP Journals

Article

Technical Report

  • Technical Report | | Open Access

    Brightfield multiplex immunohistochemistry (IHC) is improved by replacing broadly absorbing chromogens with narrowband covalently deposited chromogens, and sequentially illuminating with light channels matched to chromogen absorbance bands, synchronized with monochromatic image acquisition. Light emitting diodes provide a path to rapid multispectral imaging. Spectral unmixing provided accurate representations of biomarkers that faithfully reproduced 3,3′-diaminobenzidine IHC.

    • Larry E. Morrison
    • , Mark R. Lefever
    • , Lauren J. Behman
    • , Torsten Leibold
    • , Esteban A. Roberts
    • , Uwe B. Horchner
    •  & Daniel R. Bauer

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