Rat sperm cryopreservation and IVF
The recent emergence of tools for altering the rat genome, notably genome-editing technologies, has accelerated the generation of genetically engineered rat models for research. Efficient methods are needed for archiving and distributing these valuable strains.
In a new protocol, Takeo, Nakao et al. describe all the steps for the preparation of rat cryopreserved sperm, in vitro fertilization (IVF) using cryopreserved sperm and embryo transfer. This optimized protocol, which results in a high IVF rate in different rat strains, provides an efficient path for the preservation of rat resources.
See Takeo et al.