Volume 50

  • No. 4 April 2021

    Ischemic stroke in the brainstem

    Strokes that occur in the human brainstem are often lethal; if patients do survive, they are usually left with severe cognitive and/or motor disabilities. Animal models are critical to understanding and treating stroke, but targeting different areas of the brain, such as the brainstem, can be difficult. A new Protocol this month details the steps to induce a focal infarction in the rat brainstem.

    See Namioka et al.

  • No. 3 March 2021

    Non-invasive drug administration via micropipette

    The micropipette-guided drug administration (MDA) method was originally developed as a less-stressful alternative to oral gavage for delivering drugs to laboratory mice. New research published this month evaluates the MDA approach as a non-invasive alternative to intraperitoneal injections to deliver ligands for DREADD systems, a chemogenetic technique for manipulating neuronal activity in behaving animals.

    See Schalbetter et al.

  • No. 2 February 2021

    Sterile hybrids for inducing pseudopregnancy

    To induce pseudopregnancy in female mice, they need to be mated with males that are sterile and can’t cause actual pregnancy. Sterility can be achieved through surgical vasectomy or the use of genetically modified males but in the former case, there can be pain and discomfort involved and in the latter, unusable animals. A new research article proposes a potential refinement and reduction for inducing pseudopregnancy: mating females with naturally sterile hybrid male mice.

    See Preece et al.

  • No. 1 January 2021

    Exploring the cardiovascular effects of isoflurane in mice

    The effects of anesthetics on an animal are important considerations for both animal welfare and scientific research, with consequences for how researchers interpret results with their subjects. Isoflurane, for example, has long been thought to cause cardiovascular depression in mice. A new research article revisits this anesthetic, exploring a variety of different measures in search of the effects of isoflurane on cardiovascular function.

    See Poon et al.