Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Materials and Methods
  • Published:

Rapid PCR-Cloning of Full-Length Mouse Immunoglobulin Variable Regions

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

References

  1. Reichmann, L., Clark, M., Waldmann, H. and Winter, G. . 1988. Nature 322: 21–25.

    Google Scholar 

  2. Queen, C., Schneider, W.P., Selick, H.E. et al. 1989. Proc. Natl. Acad. Sci. USA 84: 3429–3443.

    Google Scholar 

  3. Kettleborough, D.A., Saldana, J. Heath, V.J., Morrison, C.J. and Bedig, M.M. 1990. Protein Eng. (in preparation).

    Google Scholar 

  4. Larrick, J.W., Danielsson, L., Brenner, C.A. et al. 1989. Bio/Technology 7: 934–938.

    CAS  Google Scholar 

  5. Kabat, E.A., Wu, T.T., Reid-Miller, M., Perry, H.M. and Gottesmann, K.S. 1987. US Dept Health and Human Services, US Government Printing Offices.

  6. Larrick, J.W., Coloma, M.J., Del Valle, J. and Fernandez, M.E. Scand. J. Immunol. 32: 121–128.

  7. Zwickl, M., Zaninetta, D., McMaster, G.K. and Hardman, N. 1990. J. Immunol. Methods 130: 49–55.

    Article  CAS  Google Scholar 

  8. Chaudhary, V.K., Batra, J.K., Gallo, M.G., Willingham, M.C., Fitzgerald, D.J. and Pastan, I. 1990. Proc. Natl. Acad. Sci. USA 87: 1066–1070.

    Article  CAS  Google Scholar 

  9. McClelland, M. and Nelson, M. 1987. Gene Amplification and Analysis. 5: 257–282.

  10. Anon . 1989. NEB Transcript 2: 8–9.

    Google Scholar 

  11. Orlandi, R., Gussow, D.H., Jones, P.T. and Winter, G. 1989. Proc. Natl. Acad. Sci USA 86: 3833–3837.

    Article  CAS  Google Scholar 

  12. Pech, M., Hochtl, J., Schnell, H. and Zauchau, H.G. 1981. Nature 291: 668–670.

    Article  CAS  Google Scholar 

  13. Loh, E.Y., Elliott, J.F., Cwirla, S., Lanier, L.L. and Davis, M.M. 1989. Science 243: 217–220.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jones, S., Bendig, M. Rapid PCR-Cloning of Full-Length Mouse Immunoglobulin Variable Regions. Nat Biotechnol 9, 88–89 (1991). https://doi.org/10.1038/nbt0191-88

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0191-88

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing