Browse Articles

  • Article
    | Open Access

    Qin Wang et al. show that altered phosphate metabolism contributes to the progression of kidney disease using a salt-sensitive rat model. They demonstrate that using the phosphate binder sucroferric oxyhydroxide reduces kidney injury and, using transcriptome sequencing, identify a potential role for complement C1q in kidney inflammation linked to phosphate metabolism defects.

    • Qin Wang
    • , Kenichi Ishizawa
    • , Jinping Li
    • , Wataru Fujii
    • , Yoshikazu Nemoto
    • , Osamu Yamazaki
    • , Yoshifuru Tamura
    • , Yutaka Miura
    • , Xuedan Nie
    • , Ryo Abe
    • , Hiroko Segawa
    • , Makoto Kuro-O
    •  & Shigeru Shibata
  • Article
    | Open Access

    Using a network propagation approach with integrated multi-omic data, Selevsek et al. develop a reproducible workflow for identifying drug toxicity effects in cellular systems. This is demonstrated with the analysis of anthracycline cardiotoxicity in cardiac microtissues under the effect of multiple drugs.

    • Nathalie Selevsek
    • , Florian Caiment
    • , Ramona Nudischer
    • , Hans Gmuender
    • , Irina Agarkova
    • , Francis L. Atkinson
    • , Ivo Bachmann
    • , Vanessa Baier
    • , Gal Barel
    • , Chris Bauer
    • , Stefan Boerno
    • , Nicolas Bosc
    • , Olivia Clayton
    • , Henrik Cordes
    • , Sally Deeb
    • , Stefano Gotta
    • , Patrick Guye
    • , Anne Hersey
    • , Fiona M. I. Hunter
    • , Laura Kunz
    • , Alex Lewalle
    • , Matthias Lienhard
    • , Jort Merken
    • , Jasmine Minguet
    • , Bernardo Oliveira
    • , Carla Pluess
    • , Ugis Sarkans
    • , Yannick Schrooders
    • , Johannes Schuchhardt
    • , Ines Smit
    • , Christoph Thiel
    • , Bernd Timmermann
    • , Marcha Verheijen
    • , Timo Wittenberger
    • , Witold Wolski
    • , Alexandra Zerck
    • , Stephane Heymans
    • , Lars Kuepfer
    • , Adrian Roth
    • , Ralph Schlapbach
    • , Steven Niederer
    • , Ralf Herwig
    •  & Jos Kleinjans
  • Article
    | Open Access

    Tanaka et al investigate the mechanism by which psoriasis-like dermatitis may occur following PD-1 antibody treatment for melanoma. They find that PD-1 loss in CD8 T-cells accelerates dermatitis in a manner depending on IL-6 signaling, suggesting IL-6 as a potential therapeutic target.

    • Ryota Tanaka
    • , Yuki Ichimura
    • , Noriko Kubota
    • , Akimasa Saito
    • , Yoshiyuki Nakamura
    • , Yosuke Ishitsuka
    • , Rei Watanabe
    • , Yasuhiro Fujisawa
    • , Mirei Kanzaki
    • , Seiya Mizuno
    • , Satoru Takahashi
    • , Manabu Fujimoto
    •  & Naoko Okiyama
  • Article
    | Open Access

    Hiraga et al. generate knockout mice lacking type II cadherins Cdh6, Cdh8 and Cdh11 to elucidate their role in the developing CNS. Whereas no phenotype is observed in single knockouts, the analysis of double and triple knockouts show that type II cadherins serve essential and shared roles in compartmentalization and neurulation.

    • Kou Hiraga
    • , Yukiko U. Inoue
    • , Junko Asami
    • , Mayuko Hotta
    • , Yuki Morimoto
    • , Shoji Tatsumoto
    • , Mikio Hoshino
    • , Yasuhiro Go
    •  & Takayoshi Inoue
  • Q&A
    | Open Access

    João Conde began his independent career at NOVA Medical School of Universidade Nova de Lisboa in the beginning of 2020. In this short Q&A he tells us about his experience as an early career researcher, challenges he faced with science under lockdown, the advice he has for his younger self and what is the most likely science fiction vision we can achieve with nanotechnology.

  • Article
    | Open Access

    Pazicky et al. describe the structures of glideosome trimeric complexes consisting of myosin A, essential light chain (ELC) and myosin light chain in T. gondii and P. falciparum. They show that ELCs enhance MyoA performance by inducing secondary structure in MyoA, stiffening its lever arm and calcium binding has no influence on ELC structure. These findings provide insight into this important virulence machinery.

    • Samuel Pazicky
    • , Karthikeyan Dhamotharan
    • , Karol Kaszuba
    • , Haydyn D. T. Mertens
    • , Tim Gilberger
    • , Dmitri Svergun
    • , Jan Kosinski
    • , Ulrich Weininger
    •  & Christian Löw
  • Article
    | Open Access

    Cheng, Chung et al. develop a compact multimodal, multiphoton microscope for the detection of protein crystals with high sensitivity. This new tool facilitates the identification and detection of protein micro- and nano-crystal suspensions for serial diffraction data collection at X-ray free-electron lasers or high-intensity microfocus synchrotron radiation sources.

    • Qing-di Cheng
    • , Hsiang-Yu Chung
    • , Robin Schubert
    • , Shih-Hsuan Chia
    • , Sven Falke
    • , Celestin Nzanzu Mudogo
    • , Franz X. Kärtner
    • , Guoqing Chang
    •  & Christian Betzel
  • Article
    | Open Access

    Virginie Rozot and Elisa Nemes et al. report comprehensive profiling of immune responses from participants in a recent clinical trial of the H4:IC31 and BCG vaccines against Mycobacterium tuberculosis infection. Using a novel analysis platform, they uncover complex vaccine-induced modulation of different immune cell subsets.

    • Virginie Rozot
    • , Elisa Nemes
    • , Hennie Geldenhuys
    • , Munyaradzi Musvosvi
    • , Asma Toefy
    • , Frances Rantangee
    • , Lebohang Makhethe
    • , Mzwandile Erasmus
    • , Nicole Bilek
    • , Simbarashe Mabwe
    • , Greg Finak
    • , William Fulp
    • , Ann M. Ginsberg
    • , David A. Hokey
    • , Muki Shey
    • , Sanjay Gurunathan
    • , Carlos DiazGranados
    • , Linda-Gail Bekker
    • , Mark Hatherill
    • , Thomas J. Scriba
    • , Charmaine Abrahams
    • , Marcelene Aderiye
    • , Hadn Africa
    • , Deidre Albertyn
    • , Fadia Alexander
    • , Julia Amsterdam
    • , Peter Andersen
    • , Denis Arendsen
    • , Hanlie Bester
    • , Elizabeth Beyers
    • , Natasja Botes
    • , Janelle Botes
    • , Samentra Braaf
    • , Roger Brooks
    • , Yolundi Cloete
    • , Alessandro Companie
    • , Kristin Croucher
    • , Ilse Davids
    • , Guy de Bruyn
    • , Bongani Diamond
    • , Portia Dlakavu
    • , Palesa Dolo
    • , Sahlah Dubel
    • , Cindy Elbring
    • , Ruth D. Ellis
    • , Margareth Erasmus
    • , Terence Esterhuizen
    • , Thomas Evans
    • , Christine Fattore
    • , Sebastian Gelderbloem
    • , Diann Gempies
    • , Sandra Goliath
    • , Peggy Gomes
    • , Yolande Gregg
    • , Elizabeth Hamilton
    • , Willem A. Hanekom
    • , Johanna Hector
    • , Roxanne Herling
    • , Yulandi Herselman
    • , Robert Hopkins
    • , Jane Hughes
    • , Devin Hunt
    • , Henry Issel
    • , Helene Janosczyk
    • , Lungisa Jaxa
    • , Carolyn Jones
    • , Jateel Kassiem
    • , Sophie Keffers
    • , Xoliswa Kelepu
    • , Alana Keyser
    • , Alexia Kieffer
    • , Ingrid Kromann
    • , Sandra Kruger
    • , Maureen Lambrick
    • , Bernard Landry
    • , Phumzile Langata
    • , Maria Lempicki
    • , Marie-Christine Locas
    • , Angelique Luabeya
    • , Lauren Mactavie
    • , Lydia Makunzi
    • , Pamela Mangala
    • , Clive Maqubela
    • , Boitumelo Mosito
    • , Angelique Mouton
    • , Humphrey Mulenga
    • , Mariana Mullins
    • , Julia Noble
    • , Onke Nombida
    • , Dawn O’Dee
    • , Amy O’Neil
    • , Rose Ockhuis
    • , Saleha Omarjee
    • , Fajwa Opperman
    • , Dhaval Patel
    • , Christel Petersen
    • , Abraham Pretorius
    • , Debbie Pretorius
    • , Michael Raine
    • , Rodney Raphela
    • , Maigan Ratangee
    • , Christian Rauner
    • , Susan Rossouw
    • , Surita Roux
    • , Kathryn Tucker Rutkowski
    • , Robert Ryall
    • , Elisma Schoeman
    • , Constance Schreuder
    • , Steven G. Self
    • , Cashwin September
    • , Justin Shenje
    • , Barbara Shepherd
    • , Heather Siefers
    • , Eunice Sinandile
    • , Danna Skea
    • , Marcia Steyn
    • , Jin Su
    • , Sharon Sutton
    • , Anne Swarts
    • , Patrick Syntin
    • , Michele Tameris
    • , Petrus Tyambetyu
    • , Arrie van der Merwe
    • , Elize van der Riet
    • , Dorothy van der Vendt
    • , Denise van der Westhuizen
    • , Anja van der Westhuizen
    • , Elma van Rooyen
    • , Ashley Veldsman
    • , Helen Veltdsman
    • , Emerencia Vermeulen
    • , Sindile Wiseman Matiwane
    •  & Noncedo Xoyana
  • Article
    | Open Access

    Rangrez et al. show that overexpression of the HECT domain E3 ubiquitin protein ligase 3 (HectD3) reduces cardiac hypertrophy while reducing macrophage infiltration in mice. This study provides a cardioprotective mechanism, where HectD3 targets SUMO2 and Stat1 to exert its anti-hypertrophic and anti-inflammatory effects.

    • Ashraf Yusuf Rangrez
    • , Ankush Borlepawar
    • , Nesrin Schmiedel
    • , Anushka Deshpande
    • , Anca Remes
    • , Manju Kumari
    • , Alexander Bernt
    • , Lynn Christen
    • , Andreas Helbig
    • , Andreas Jungmann
    • , Samuel Sossalla
    • , Andreas Tholey
    • , Oliver J. Müller
    • , Derk Frank
    •  & Norbert Frey
  • Article
    | Open Access

    Eduardo Moralejo et al. report a phylogenetic reconstruction tracing the origin and progression of a European outbreak of the almond scorch disease pathogen Xylella fastidiosa (Xf). Their data suggest Xf was introduced into Europe via grafting from infected Californian buds and was subsequently spread by the meadow spittlebug to multiple plant hosts.

    • Eduardo Moralejo
    • , Margarita Gomila
    • , Marina Montesinos
    • , David Borràs
    • , Aura Pascual
    • , Alicia Nieto
    • , Francesc Adrover
    • , Pere A. Gost
    • , Guillem Seguí
    • , Antonio Busquets
    • , José A. Jurado-Rivera
    • , Bàrbara Quetglas
    • , Juan de Dios García
    • , Omar Beidas
    • , Andreu Juan
    • , María P. Velasco-Amo
    • , Blanca B. Landa
    •  & Diego Olmo
  • Article
    | Open Access

    Yang, Cui, Zhang et al. report an optogenetic nanosystem which controls the secretion of immune factors by engineered light-responsive bacteria for systemic immunity regulation. On shining NIR light on mice’s stomach, blue light emission from upconversion rods induce excretion of TGF-β1 and IFN-γ from the bacteria, thereby suppressing intestinal inflammatory response or slowing down progress of subcutaneously transplanted tumour, respectively.

    • Chun Yang
    • , Meihui Cui
    • , Yingying Zhang
    • , Huizhuo Pan
    • , Jing Liu
    • , Shixing Wang
    • , Ning Ma
    • , Jin Chang
    • , Tao Sun
    •  & Hanjie Wang
  • Article
    | Open Access

    Alma Andersson et al. present a probabilistic framework that integrates single-cell and bulk spatial transcriptomics in order to spatially map cell types onto their respective tissues. They apply their method to the developing human heart and mouse brain to demonstrate the power of the technique.

    • Alma Andersson
    • , Joseph Bergenstråhle
    • , Michaela Asp
    • , Ludvig Bergenstråhle
    • , Aleksandra Jurek
    • , José Fernández Navarro
    •  & Joakim Lundeberg
  • Article
    | Open Access

    Koen van de Ven and colleagues report that exposure to H1N1 influenza in ferrets results in T-cell-mediated immune responses to pandemic H2N2. This indicates that T cells may be important mediators of cross-protection against different influenza A strains and may have important implications for vaccine design.

    • Koen van de Ven
    • , Femke de Heij
    • , Harry van Dijken
    • , José A. Ferreira
    •  & Jørgen de Jonge
  • Article
    | Open Access

    Using a genome-wide association study approach, Chaguza et al. identify significant genotype-phenotype associations relevant to Streptococcus pneumoniae infection. These findings indicate genetic variations in the pathogen attributed to pneumococcal tropism to central nervous system tissues, with implications for meningitis virulence.

    • Chrispin Chaguza
    • , Marie Yang
    • , Jennifer E. Cornick
    • , Mignon du Plessis
    • , Rebecca A. Gladstone
    • , Brenda A. Kwambana-Adams
    • , Stephanie W. Lo
    • , Chinelo Ebruke
    • , Gerry Tonkin-Hill
    • , Chikondi Peno
    • , Madikay Senghore
    • , Stephen K. Obaro
    • , Sani Ousmane
    • , Gerd Pluschke
    • , Jean-Marc Collard
    • , Betuel Sigaùque
    • , Neil French
    • , Keith P. Klugman
    • , Robert S. Heyderman
    • , Lesley McGee
    • , Martin Antonio
    • , Robert F. Breiman
    • , Anne von Gottberg
    • , Dean B. Everett
    • , Aras Kadioglu
    •  & Stephen D. Bentley
  • Article
    | Open Access

    Kawaguchi et al. show that mice deficient in lipocalin-type prostaglandin (PG) D synthase (L-PGDS) exhibit impaired light-induced phase advance, but normal phase delay and nonvisual light responses. This study suggests the role of L-PGDS for the light-induced phase advance possibly via a chemoattractant receptor DP2/CRTH2.

    • Chihiro Kawaguchi
    • , Norihito Shintani
    • , Atsuko Hayata-Takano
    • , Michiyoshi Hatanaka
    • , Ai Kuromi
    • , Reiko Nakamura
    • , Yui Yamano
    • , Yusuke Shintani
    • , Katsuya Nagai
    • , Soken Tsuchiya
    • , Yukihiko Sugimoto
    • , Atsushi Ichikawa
    • , Yasushi Okuno
    • , Yoshihiro Urade
    • , Hiroyuki Hirai
    • , Kin-ya Nagata
    • , Masataka Nakamura
    • , Shuh Narumiya
    • , Takanobu Nakazawa
    • , Atsushi Kasai
    • , Yukio Ago
    • , Kazuhiro Takuma
    • , Akemichi Baba
    •  & Hitoshi Hashimoto
  • Article
    | Open Access

    Zhao et al. show that caffeine restores Wnt signaling activity by suppressing Notum. The high-resolution crystal structure of Notum bound with caffeine suggests that caffeine may compete with Notum’s natural substrate. Structural insights from this study may therapeutically benefit several pathologies including Alzheimer’s disease.

    • Yuguang Zhao
    • , Jingshan Ren
    • , James Hillier
    • , Weixian Lu
    •  & Edith Yvonne Jones
  • Article
    | Open Access

    Michinobu Kuwae et al. reconstruct the past dynamics of pelagic fish abundance in Beppu Bay of Japan, using sedimentary DNA. This study demonstrates the utility of sedimentary DNA in investigating the history of fish abundance in marine waters.

    • Michinobu Kuwae
    • , Hiromichi Tamai
    • , Hideyuki Doi
    • , Masayuki K. Sakata
    • , Toshifumi Minamoto
    •  & Yoshiaki Suzuki
  • Article
    | Open Access

    Using Msr1 knockout mice, Long Yang et al. demonstrate that macrophage scavenger receptor 1 (MSR1) activates autophagy to restrict the proliferation of Chikungunya virus, an alphavirus that causes crippling joint stiffness. This study provides insights into how host cellular machinery fights off Chikungunya virus.

    • Long Yang
    • , Tingting Geng
    • , Guang Yang
    • , Jinzhu Ma
    • , Leilei Wang
    • , Harshada Ketkar
    • , Duomeng Yang
    • , Tao Lin
    • , Jesse Hwang
    • , Shu Zhu
    • , Yanlin Wang
    • , Jianfeng Dai
    • , Fuping You
    • , Gong Cheng
    • , Anthony T. Vella
    • , Richard. A. Flavell
    • , Erol Fikrig
    •  & Penghua Wang
  • Author Correction
    | Open Access

    • Takafumi Suzuki
    • , Akira Uruno
    • , Akane Yumoto
    • , Keiko Taguchi
    • , Mikiko Suzuki
    • , Nobuhiko Harada
    • , Rie Ryoke
    • , Eriko Naganuma
    • , Nanae Osanai
    • , Aya Goto
    • , Hiromi Suda
    • , Ryan Browne
    • , Akihito Otsuki
    • , Fumiki Katsuoka
    • , Michael Zorzi
    • , Takahiro Yamazaki
    • , Daisuke Saigusa
    • , Seizo Koshiba
    • , Takashi Nakamura
    • , Satoshi Fukumoto
    • , Hironobu Ikehata
    • , Keizo Nishikawa
    • , Norio Suzuki
    • , Ikuo Hirano
    • , Ritsuko Shimizu
    • , Tetsuya Oishi
    • , Hozumi Motohashi
    • , Hirona Tsubouchi
    • , Risa Okada
    • , Takashi Kudo
    • , Michihiko Shimomura
    • , Thomas W. Kensler
    • , Hiroyasu Mizuno
    • , Masaki Shirakawa
    • , Satoru Takahashi
    • , Dai Shiba
    •  & Masayuki Yamamoto
  • Editorial
    | Open Access

    The metabolism of immune cells is a rapidly developing field with therapeutic implications. In recognition of this exciting area, our journal is welcoming submissions of primary research articles, perspectives, comments, and review articles in immunometabolism with the aim to highlight these articles in a special collection.

    • Shuai Jiang
  • Article
    | Open Access

    Gurska et al. dissect the expression and function of zen genes (Hox3 in mammals) in the beetle Tribolium castaneum. They identify dynamic regulatory interactions of the tandemly duplicated paralogues that support extraembryonic development, under a scenario of evolutionary subfunctionalization.

    • Daniela Gurska
    • , Iris M. Vargas Jentzsch
    •  & Kristen A. Panfilio
  • Article
    | Open Access

    Ambattu et al. report that high frequency acoustic stimulation of mammalian cancer cells promotes exosome generation without affecting cell viability. Acoustic stimulation increases calcium ion influx which triggers an ESCRT pathway related to exosome production. This method may allow for large-scale exosome production with potential clinical applications.

    • Lizebona August Ambattu
    • , Shwathy Ramesan
    • , Chaitali Dekiwadia
    • , Eric Hanssen
    • , Haiyan Li
    •  & Leslie Y. Yeo
  • Article
    | Open Access

    Rochman et al. present deep phylogenies for 84 cancer driver genes and examine the prevalence of driver mutations across gene-species trees. Their results show that driver mutations are rare in species evolution and give insight into the evolution of driver mutations and oncogenes.

    • Nash D. Rochman
    • , Yuri I. Wolf
    •  & Eugene V. Koonin
  • Article
    | Open Access

    Gault et al. show that high pressures increase α-chymotrypsin enzyme activity in the presence of high perchlorate concentrations. These perchlorate salts shift the folded enzyme phase space to lower temperatures and pressure and may move the optimum enzyme activity towards lower temperatures in addition to higher pressures, which has implications for Martian habitability.

    • Stewart Gault
    • , Michel W. Jaworek
    • , Roland Winter
    •  & Charles S. Cockell
  • Article
    | Open Access

    Robin Lardon et al. report a genome-wide association study of shoot regeneration in Arabidopsis under 2 different in vitro incubation conditions. They find wide variation in regeneration phenotypes, attributable to allelic variants in key developmental genes, and show that genetic association patterns differ depending on environmental factors.

    • Robin Lardon
    • , Erik Wijnker
    • , Joost Keurentjes
    •  & Danny Geelen
  • Article
    | Open Access

    Tamminen et al. show that a commercial, tube-source µCT device combined with computational image analysis can be used to obtain quantitative 3D data for cellular structures in optically-challenging samples hard to image using other methods.

    • Ilmari Tamminen
    • , Kalle Lehto
    • , Markus Hannula
    • , Miina Ojansivu
    • , Laura Johansson
    • , Minna Kellomäki
    • , Susanna Miettinen
    • , Antti Aula
    • , Teemu Ihalainen
    •  & Jari Hyttinen
  • Article
    | Open Access

    Ying Zheng et al. characterize MYC gene and protein expression in single mammalian cells in response to various external signals. They find that individual cells show either high or low basal MYC expression setpoints, and that adherence to these setpoints as well as the magnitude of the response of MYC to stimulation, is controlled by FUBP1 and FUBP2.

    • Ying Zheng
    • , Wendy Dubois
    • , Craig Benham
    • , Eric Batchelor
    •  & David Levens
  • Article
    | Open Access

    Conciatori et al find that BRAF mutations and PTEN-loss promote IL-8 production in colorectal cancer cell (CRC) lines and identify a genetic-context-dependent BRAF/ERK2/CHOP molecular axis that controls IL-8 transcription. These data may assist in the identification of drugs to target CRC.

    • Fabiana Conciatori
    • , Chiara Bazzichetto
    • , Carla Azzurra Amoreo
    • , Isabella Sperduti
    • , Sara Donzelli
    • , Maria Grazia Diodoro
    • , Simonetta Buglioni
    • , Italia Falcone
    • , Senji Shirasawa
    • , Giovanni Blandino
    • , Gianluigi Ferretti
    • , Francesco Cognetti
    • , Michele Milella
    •  & Ludovica Ciuffreda
  • Article
    | Open Access

    Hyunjin Cho et al. show that peptidyl compounds activating the periplasmic DegP protease inhibit the growth of Escherichia coli by promoting the proteolysis of newly synthesized proteins. This study presents an intriguing strategy to combat antibiotic resistance by activating a non-essential bacterial enzyme, thus expanding the scope of traditional antibiotic targets.

    • Hyunjin Cho
    • , Yuri Choi
    • , Kyungjin Min
    • , Jung Bae Son
    • , Hyojin Park
    • , Hyung Ho Lee
    •  & Seokhee Kim
  • Article
    | Open Access

    Hahn, van Krieken et al. provide a quantitative account of β-cell mass dynamics and islet longevity in mice treated with Streptozotocin (STZ). They find that STZ-induced hyperglycemia primarily results from β-cell dysfunction rather than its ablation. This study provides insights into how the most widely used preclinical diabetes model works.

    • Max Hahn
    • , Pim P. van Krieken
    • , Christoffer Nord
    • , Tomas Alanentalo
    • , Federico Morini
    • , Yan Xiong
    • , Maria Eriksson
    • , Jürgen Mayer
    • , Elena Kostromina
    • , Jorge L. Ruas
    • , James Sharpe
    • , Teresa Pereira
    • , Per-Olof Berggren
    • , Erwin Ilegems
    •  & Ulf Ahlgren
  • Article
    | Open Access

    Cheatle Jarvela et al. demonstrate in the mosquito Anopheles stephensi that the paired gene was functionally replaced by the gene gooseberry, even though paired is essential in other insects such as fruit flies and beetles. This study contributes to the understanding of how essential genes are lost despite their importance during development.

    • Alys M. Cheatle Jarvela
    • , Catherine S. Trelstad
    •  & Leslie Pick
  • Article
    | Open Access

    Yasuo Kubota et al. report a multi-omic analysis of pediatric germ cell tumors from 51 patients ranging in age from 2 months to 19 years. They identify unique methylation, expression, and mutational patterns for each of the main subtypes and propose potential target genes for treatments against the three main subtypes.

    • Yasuo Kubota
    • , Masafumi Seki
    • , Tomoko Kawai
    • , Tomoya Isobe
    • , Misa Yoshida
    • , Masahiro Sekiguchi
    • , Shunsuke Kimura
    • , Kentaro Watanabe
    • , Aiko Sato-Otsubo
    • , Kenichi Yoshida
    • , Hiromichi Suzuki
    • , Keisuke Kataoka
    • , Yoichi Fujii
    • , Yuichi Shiraishi
    • , Kenichi Chiba
    • , Hiroko Tanaka
    • , Mitsuteru Hiwatari
    • , Akira Oka
    • , Yasuhide Hayashi
    • , Satoru Miyano
    • , Seishi Ogawa
    • , Kenichiro Hata
    • , Yukichi Tanaka
    •  & Junko Takita
  • Article
    | Open Access

    Ronald Biggs et al. report biophysical measurements of intact chromosomes isolated from mouse spermatocytes. They compare chromosomes in meiosis prophase I to mitotic chromosomes and find that meiotic chromosomes are much stiffer, and this stiffness does not depend on the central element of the synaptonemal complex (SYCP1).

    • Ronald J. Biggs
    • , Ning Liu
    • , Yiheng Peng
    • , John F. Marko
    •  & Huanyu Qiao
  • Article
    | Open Access

    Godino et al. show that FtsA-FtsZ ring-like structures driven by cell-free gene expression can be reconstituted on planar membranes and inside liposome compartments. These cytoskeletal structures constrict the liposome, generating elongated membrane necks and budding vesicles. This study represents a step forward to realizing genetic programming of synthetic cell division.

    • Elisa Godino
    • , Jonás Noguera López
    • , Ilias Zarguit
    • , Anne Doerr
    • , Mercedes Jimenez
    • , Germán Rivas
    •  & Christophe Danelon
  • Article
    | Open Access

    Katerina Trajanoska et al. report a genome-wide association study of self-reported falls in UK Biobank participants. They identify three novel fall-associated loci and find that risk of falling shows patterns of polygenic inheritance and a SNP-based heritability of 2.7%.

    • Katerina Trajanoska
    • , Lotta J. Seppala
    • , Carolina Medina-Gomez
    • , Yi-Hsiang Hsu
    • , Sirui Zhou
    • , Natasja M. van Schoor
    • , Lisette C. P. G. M. de Groot
    • , David Karasik
    • , J. Brent Richards
    • , Douglas P. Kiel
    • , Andre G. Uitterlinden
    • , John R. B. Perry
    • , Nathalie van der Velde
    • , Felix R. Day
    •  & Fernando Rivadeneira
  • Article
    | Open Access

    Samarakoon et al. present Genopo, a portable toolkit for analysis of nanopore sequencing data on Android smartphone and tablet devices. Genopo enables field-based nanopore analysis using widely accessible technology.

    • Hiruna Samarakoon
    • , Sanoj Punchihewa
    • , Anjana Senanayake
    • , Jillian M. Hammond
    • , Igor Stevanovski
    • , James M. Ferguson
    • , Roshan Ragel
    • , Hasindu Gamaarachchi
    •  & Ira W. Deveson
  • Article
    | Open Access

    Amblard et al combine the manipulation of H2O2 levels with the use of a H2O2 sensor to show that altering endogenous H2O2 levels affects the distribution of the midbrain pattering factor Engrailed 2 in Zebrafish during tectum development. They also find that Engrailed 2 internalization increases H2O2 levels suggesting a feedback mechanism.

    • Irène Amblard
    • , Marion Thauvin
    • , Christine Rampon
    • , Isabelle Queguiner
    • , Valeriy V. Pak
    • , Vsevolod Belousov
    • , Alain Prochiantz
    • , Michel Volovitch
    • , Alain Joliot
    •  & Sophie Vriz
  • Article
    | Open Access

    Weng Kung Peng et al. present a novel scheme using two-dimensional NMR spectroscopy for rapid and label-free testing of biological fluids (e.g., red blood cells) at point-of-care. They demonstrate its clinical utility (e.g., with haemoglobin disorders) and report a unique and specific ‘molecular fingerprint’ through direct analysis of a single drop of blood (<5 µl). Machine learning is used to facilitate simpler decision making.

    • Weng Kung Peng
    • , Tian-Tsong Ng
    •  & Tze Ping Loh
  • Article
    | Open Access

    Daisley et al. show that antibiotic treatment with oxytetracycline impairs the gut microbiota and immune system of honey bees, and reduces capped brood counts. They also show that supplementation with lactobacilli during antibiotic recovery can reverse the harmful effects of the antibiotic treatment. Their findings offer a simple microbial-based solution that aims to reduce honey bee disease burden, environmental pollution by xenobiotics, and spread of antimicrobial resistance.

    • Brendan A. Daisley
    • , Andrew P. Pitek
    • , John A. Chmiel
    • , Shaeley Gibbons
    • , Anna M. Chernyshova
    • , Kait F. Al
    • , Kyrillos M. Faragalla
    • , Jeremy P. Burton
    • , Graham J. Thompson
    •  & Gregor Reid
  • Article
    | Open Access

    Julien Brechbühl et al. show that the Grueneberg ganglion olfactory subsystem is necessary for deciphering the threatening or safe qualities of unfamiliar food based on olfactory or social signals, respectively, in mice. These results highlight the role of this subsystem in optimizing decision-making strategies related to food preference by integrating environmental cues.

    • Julien Brechbühl
    • , Aurélie de Vallière
    • , Dean Wood
    • , Monique Nenniger Tosato
    •  & Marie-Christine Broillet
  • Article
    | Open Access

    Ronghui Song et al. demonstrate that a lawsone (2-hydroxy-1,4-naphthoquinone)-based compound decreases the drug resistance of methicillin-resistant Staphylococcus aureus much better than conventional antibiotics. This study provides insights into the design and action mechanism of effective antibiotics that overcome the antibiotic resistance of bacteria.

    • Ronghui Song
    • , Bing Yu
    • , Dirk Friedrich
    • , Junfeng Li
    • , Hao Shen
    • , Harald Krautscheid
    • , Songping D. Huang
    •  & Min-Ho Kim
  • Article
    | Open Access

    Shao et al. show that a bacterial symbiont drives its fungal host to biosynthesize a polyketide antimicrobial, spiromarmycin, fending off their competitors. They find that the spiromarmycin biosynthetic machinery is encoded by the fungal host. This study provides insights into the evolution of marine microbial diversity.

    • Mingwei Shao
    • , Changli Sun
    • , Xiaoxiao Liu
    • , Xiaoxue Wang
    • , Wenli Li
    • , Xiaoyi Wei
    • , Qinglian Li
    •  & Jianhua Ju
  • Article
    | Open Access

    With NMR and MD simulations, Li, Chen et al. comprehensively probe the effects of osmolytes on hydrogen bond formation in proteins. They show that polyol and sugar osmolytes weaken hydrogen bonding between the protein and solvent while strengthening those within the protein.

    • Jingwen Li
    • , Jingfei Chen
    • , Liaoyuan An
    • , Xiaoxiang Yuan
    •  & Lishan Yao
  • Article
    | Open Access

    Gen Sobue, Masao Nagasaki and colleagues report a genome-wide association study for amyotrophic lateral sclerosis (ALS) in a large, multi-ethnic cohort comprising Japanese, Chinese, and European ancestry populations. They find a significant association to variants within the ACSL5 gene and identify novel associations with 4 additional genes using a gene-based approach.

    • Ryoichi Nakamura
    • , Kazuharu Misawa
    • , Genki Tohnai
    • , Masahiro Nakatochi
    • , Sho Furuhashi
    • , Naoki Atsuta
    • , Naoki Hayashi
    • , Daichi Yokoi
    • , Hazuki Watanabe
    • , Hirohisa Watanabe
    • , Masahisa Katsuno
    • , Yuishin Izumi
    • , Kazuaki Kanai
    • , Nobutaka Hattori
    • , Mitsuya Morita
    • , Akira Taniguchi
    • , Osamu Kano
    • , Masaya Oda
    • , Kazumoto Shibuya
    • , Satoshi Kuwabara
    • , Naoki Suzuki
    • , Masashi Aoki
    • , Yasuyuki Ohta
    • , Toru Yamashita
    • , Koji Abe
    • , Rina Hashimoto
    • , Ikuko Aiba
    • , Koichi Okamoto
    • , Kouichi Mizoguchi
    • , Kazuko Hasegawa
    • , Yohei Okada
    • , Tomohiko Ishihara
    • , Osamu Onodera
    • , Kenji Nakashima
    • , Ryuji Kaji
    • , Yoichiro Kamatani
    • , Shiro Ikegawa
    • , Yukihide Momozawa
    • , Michiaki Kubo
    • , Noriko Ishida
    • , Naoko Minegishi
    • , Masao Nagasaki
    •  & Gen Sobue
  • Research Highlight
    | Open Access

    While polygyny is common among vertebrates, polygyny with mate fidelity has not yet been demonstrated in amphibians. A recent study by Fábio de Sá and colleagues shows that single male saxicolous frogs share a breeding territory with two females and mate multiple times with them over the course of a breeding season. These authors attribute the evolution of this mating system to the intense competition for territories and mates when access to these resources is scarce.

    • Caitlin Karniski
  • Article
    | Open Access

    Kevin Neil et al. quantified horizontal gene transfer rates for 13 conjugative plasmids in the mouse gut microbiota to identify those that might be useful for developing microbiome editing technologies. They find that the Incl-family plasmid TP114 has a nearly 100% transfer efficiency in the mouse gut.

    • Kevin Neil
    • , Nancy Allard
    • , Frédéric Grenier
    • , Vincent Burrus
    •  & Sébastien Rodrigue
  • Article
    | Open Access

    Using T-jump 2D-IR spectroscopy, Zhang et al. show that DNA minor-groove binder Hoechst 33258 strongly destabilizes its complex with DNA (H-DNA). This study suggests that the association of H-DNA is a concerted process, where Hoechst 33258 cooperates with DNA single strands in forming H-DNA.

    • Xin-Xing Zhang
    • , Shelby L. Brantley
    • , Steven A. Corcelli
    •  & Andrei Tokmakoff
  • Article
    | Open Access

    Krishnan et al. find that CCR1 ligands CCL6 and CCL23 secreted by murine and human macrophages, respectively, enhance metastatic colonization of ovarian cancer cells to the omentum in manner dependent on chemokine receptor 1 (CCR1). This study suggests that targeting CCR1 or CCL23 in ovarian cancer may be a therapeutic strategy.

    • Venkatesh Krishnan
    • , Supreeti Tallapragada
    • , Bruce Schaar
    • , Kalika Kamat
    • , Anita M. Chanana
    • , Yue Zhang
    • , Sonia Patel
    • , Vinita Parkash
    • , Carrie Rinker-Schaeffer
    • , Ann K. Folkins
    • , Erinn B. Rankin
    •  & Oliver Dorigo