Special |

Single-cell biology

The realization that cells are the basic unit of all life helped to transform biology 180 years ago. Now, new technologies are allowing biologists to peer under the hood of individual cells from complex organisms one at a time. Their work is revealing cells’ histories and their dizzying diversity and could lead to new therapeutic approaches to disease.

Features and Comment

Aviv Regev is a maven of hard-core biological analyses. Now she is part of an effort to map every cell in the human body.

News Feature | | Nature

Analysing the DNA, RNA and protein of single cells is transforming our understanding of the immune system, say Amir Giladi and Ido Amit.

Comment | | Nature

Tools and tips

Research

Eukaryotic chromosomes undergo a cycle of compaction and decondensation during the cell cycle. Here, Peter Fraser and colleagues have developed an improved single-cell Hi-C method to characterize the 3D organization of chromosomes through the cell cycle in thousands of individual mouse embryonic stem cells. They find that chromosomal compartments, topological-associated domains and loops are each governed by distinct dynamics and reveal a continuum of dynamic chromosomal structural features throughout the cell cycle. The results will be a new point of reference for interpreting chromosome conformation Hi-C maps.

News & Views | | Nature

Eukaryotic chromosomes undergo a cycle of compaction and decondensation during the cell cycle. Here, Peter Fraser and colleagues have developed an improved single-cell Hi-C method to characterize the 3D organization of chromosomes through the cell cycle in thousands of individual mouse embryonic stem cells. They find that chromosomal compartments, topological-associated domains and loops are each governed by distinct dynamics and reveal a continuum of dynamic chromosomal structural features throughout the cell cycle. The results will be a new point of reference for interpreting chromosome conformation Hi-C maps.

Article | | Nature