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Yeast 2.0

Synthetic biology aims to redesign and reconstruct biological systems for new, useful end goals.  One of the ambitious projects currently underway is Sc2.0: the design and synthesis of a complete eukaroyotic genome - Saccharomyces cerevisiae.

This collection highlights the experimental work published in Nature Communications on redesigning the Scerevisiae genome along with commentary from the community about the potential applications and implications of this work for synthetic biology, biotechnology and our understanding of the genome. 

Commentary and Perspectives

The Sc2.0 project has set out to synthesise the Saccharomyces cerevisiae genome, with each chromosome redesigned along agreed principles. In this collection of papers, the researchers involved show how SCRaMbLE—Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution—can be used to rapidly reorganise the genome.

Editorial | Open Access | | Nature Communications


Self-propagating drives allow for non-Mendelian inheritance. Here the authors use CRISPR to build a chromosome drive, showing elimination of entire chromosomes, endoreduplication of desired chromosomes and enabling preferential transmissions of complex genetic traits on a chromosomal scale in yeast.

Article | Open Access | | Nature Communications

Genome structural variation can play an important functional role in phenotypic diversity. Here the authors use the SCRaMbLE system on a ring synthetic chromosome V to generate complex rearrangements distinct from a rearranged linear chromosome.

Article | Open Access | | Nature Communications

SCRaMbLE has been used to rearrange synthetic chromosomes that have been introduced into host yeast. Here the authors produce semi-synthetic heterozygous diploid strains for rapid selection of phenotypes and map the rearrangements underlying selected phenotypes such as thermoresistance and caffeine resistance.

Article | Open Access | | Nature Communications

SCRaMbLE allows for the rapid and large scale rearrangement of genetic data in yeast carrying synthetic chromosomes. Here the authors demonstrate an in vitro use of the method to generate DNA libraries for optimization of biochemical reactions.

Article | Open Access | | Nature Communications