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3D printing is widely known for its versatility in creating custom thermoplastic objects from computer models, but the methods and materials are becoming increasingly versatile. As printing methods continue to become more accessible, applications of the technology are now being considered throughout the life sciences – from improving research methods, to surgical interventions and communication about healthcare possibilities. This collection brings together papers that have applied 3D printing technology to problems in clinical and life sciences research, using both traditional and biological materials.