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The c-Jun N-terminal kinase prevents oxidative stress induced by UV and thermal stresses in corals and human cells

Scientific Reports volume 7, Article number: 45713 (2017) | Download Citation

Abstract

Coral reefs are of major ecological and socio-economic interest. They are threatened by global warming and natural pressures such as solar ultraviolet radiation. While great efforts have been made to understand the physiological response of corals to these stresses, the signalling pathways involved in the immediate cellular response exhibited by corals remain largely unknown. Here, we demonstrate that c-Jun N-terminal kinase (JNK) activation is involved in the early response of corals to thermal and UV stress. Furthermore, we found that JNK activity is required to repress stress-induced reactive oxygen species (ROS) accumulation in both the coral Stylophora pistillata and human skin cells. We also show that inhibiting JNK activation under stress conditions leads to ROS accumulation, subsequent coral bleaching and cell death. Taken together, our results suggest that an ancestral response, involving the JNK pathway, is remarkably conserved from corals to human, protecting cells from the adverse environmental effects.

Introduction

Living organisms have developed adaptive mechanisms allowing them to survive in a constantly changing environment1,2. Organisms that can rapidly adjust to these changes are likely to exhibit a greater degree of adaptation to a variety of stresses3. At the molecular level, cells have thus implemented complex signalling pathways to sense danger, and trigger a sequence of events that will mitigate any adverse effects. Among these pathways, mitogen-activated protein kinases (MAPKs) stimulate the expression of genes involved in the response to stress4. MAPKs also control key cellular processes such as proliferation, differentiation, migration, survival and apoptosis5. The MAPK gene family encompasses three major subgroups: the extracellular signal regulated kinase (ERK), the p38/HOG and the c-Jun N-terminal kinase (JNK) groups. JNKs are evolutionarily conserved and are found in all eukaryotes, including plants, vertebrates and anthozoans6,7. When activated by dual phosphorylation of a threonine-proline-tyrosine (TPY) motif, this family of kinases plays a key role in the response to thermal and ultra-violet radiation (UVR) stresses in animals as well as in several terrestrial and marine plants8,9,10,11,12,13.

In human skin cells, which are often exposed to high solar radiation, UVR-induced reactive oxygen species (ROS) have been suggested to induce JNK activation14, leading in turn to upregulation of anti-oxidant enzymes to overcome ROS accumulation4,13,15. Moreover, in cells, JNK has been proposed to have both pro-survival and pro-apoptotic activities, depending on the duration and intensity of the stress as well as the cellular context being examined16,17. Due to these different outcomes following activation of the JNK pathway, the primary function of JNK in the early response to UVR and thermal stress is still unclear16,18,19,20.

Tropical corals are also exposed to high solar UVR and high temperature levels, especially in the face of climate change, which have already led to more than 20% destruction of coral reefs21,22. Exposure to these stresses leads to coral bleaching, i.e. the loss of coral algal symbionts and/or photosynthetic pigments23,24, resulting in a drop in photosynthesis and calcification25, and possibly leading to coral death. At the cellular level, bleaching has been proposed to be the consequence of an elevated production of ROS which damages cell components26. To prevent or to fight oxidative stress, corals have implemented defence strategies such as the synthesis of fluorescent pigments, heat-shock proteins, mycosporin-like amino acids27,28 and the production of anti-oxidant enzymes26. An increasing number of studies indicate that corals express genes encoding MAPKs, including those encoding JNK29,30,31,32,33,34. This suggests the presence of a conserved stress response between coral and human cells at the molecular level, although the phylum cnidaria diverged from bilateria 550 million years ago35. An increase of the expression of genes coding for canonical substrates of JNK (c-Jun and c-Fos) was indeed observed in the coral Acropora sp. under thermal stress and following wounding34,36,37. Such induction may be specific to corals since post-translational and not transcriptional regulation of JNK signalling is most often observed in mammalian cells38. Although the above studies suggest a conservation of the stress response involving MAPK signalling cascades in corals, their specific functions have not been clearly defined. In particular, MAPK signalling pathway activity, which is classically considered the most appropriate readout, has never been monitored in corals.

Taking advantage of the amino acid sequence conservation of coral and human JNK orthologs, in particular the phosphorylatable TPY motif contained in the kinase domain, we used commercially available antibodies to analyse JNK activation upon thermal and UVR stress in both corals and human skin fibroblasts. Our aims were to confirm the presence of the JNK protein in corals, to study its phosphorylation (i.e. activation) under thermal and UVR stress, and to compare JNK activation in corals and human fibroblasts. In addition, we also endeavoured to link the JNK pathway activation to the defence against stress-induced ROS production in both models.

Results

Analysis of anthozoan transcriptomes

The human protein sequence for MAPK8/JNK1 (NCBI reference sequence: NM_001323322.1) was blasted against sequence databases including Stylophora pistillata39 (GENBANK: GARY01004588), Acropora digitifera40 (NCBI reference sequence: XM_015907276), Hydra vulgaris41 (NCBI reference sequence: NM_001309762) and the coral symbiotic unicellular algae Symbiodinium clade B142 (accession number symbB.v1.2.036439). A sequence showing 89%, 74% and 64% homology with the protein sequences of A. digitifera (Ad-JNK), H. vulgaris (Hv-JNK) and the human MAPK8/JNK1 (Hs-JNK1), respectively, was identified in S. pistillata. This sequence (hereafter referred to as Spi-JNK for S. pistillata JNK) contains both the conserved kinase domains43 and the TPY motif unique for JNK orthologs15, like Ad-JNK, Hv-JNK and Hs-JNK sequences (Fig. 1). These results suggest that a single ortholog of JNK is present in S. pistillata, as opposed to the three genes encoding several JNK isoforms in the human genome44. Note that the unique and closest ortholog present in the Symbiodinium sequence database showed 35% homology with Spi-JNK (Fig. 1) and contained a TEY motif characteristic of ERK, instead of a TPY motif.

Figure 1: Sequence alignment of MAPK orthologs.
Figure 1

The JNK orthologs of Stylphora pistillata (Spi-JNK), Acropora digitifera (Ad-JNK), Hydra vulgaris (Hv-JNK), the human MAPK8/JNK1 (Hs-JNK1) and the Symbiodinium sp. ERK ortholog (zoox_ERK) protein sequences are shown. The JNK-specific TPY activation motif and the ERK-specific TEY motif are highlighted in red and green respectively. The eleven conserved kinase domains are underlined.

The TPY domain of JNK is phosphorylated by kinases upstream in its signal transduction pathway and as such its phosphorylation is an established readout of JNK activation. We therefore hypothesized that Spi-JNK, like its human counterpart, is activated by phosphorylation of the threonine and tyrosine residues contained in the TPY motif. To investigate this, we used commercially available anti-human JNK antibodies to test whether phosphorylated JNK (pJNK) could be detected in S. pistillata extracts. We used human fibroblasts to validate our approach and took advantage of the absence of a JNK ortholog in the Symbiodinium genome (Fig. 1) to prevent any misinterpretation due to potential cross contamination of animal and algae proteins in total protein extracts.

Characterization of JNK activation in corals and human cells

In corals, in agreement with the transcriptome analysis, only one JNK protein was present and therefore expressed in the western blots (Fig. 2a), while, in human fibroblasts, only JNK1 and JNK2 proteins were expressed (demonstrated by siRNA knockdown experiments, Fig. 2b and Fig. S1). In control culture conditions, phosphorylated forms of JNK (pJNK) were barely detectable in fibroblasts and undetectable in coral extracts (Fig. 2a and b). However, anisomycin, an activator of JNK45, induced significant phosphorylation of JNK in both fibroblasts and S. pistillata (Fig. 2a and b). In fibroblasts, anisomycin induced the phosphorylation of isoforms that are not detected in the control condition.The anti-human pJNK antibody detected a unique immuno-reactive band in coral extracts, suggesting that this antibody recognized the phosphorylated TPY motif present in Spi-JNK (Fig. 2b). Consistent with our results indicating the absence of a JNK gene in the symbiont genome, isolated symbiont extracts did not present any immuno-reactive band when probed with the pJNK antibody (Supplementary Fig. S2). To further determine the specificity of the anti-human pJNK, we used the pharmacological inhibitor SP600125, commonly used as a JNK inhibitor46,47. It prevents autophosphorylation of JNK through competition with ATP, and the subsequent phosphorylation of JNK targets48. Treatment with SP600125 reduced the basal pJNK level in fibroblasts (Fig. 2a) and strongly reduced anisomycin induced of JNK phosphorylation in both S. pistillata and fibroblasts (Fig. 2a and b).

Figure 2: Detection of JNK activation in human fibroblasts and corals.
Figure 2

Immunoblot revealing activated (pJNK) and total forms of JNK (JNK) present in (a) human fibroblasts and (b) S. pistillata nubbins incubated in the absence (control; ct) or presence of SP600125 (SP), anisomycin (Ani), or combination of both (Ani + SP). Molecular weight standards in kilodaltons (kDa) are indicated on the right side of the blots. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of at least three independent experiments.

Both Spi- and Hs-JNK are activated by a combined thermal and UVR stress

To monitor the kinetics of JNK activation upon modifications of culture conditions, we subjected fibroblasts and coral nubbins to a combination of heat shock and UVR stress. In human cells, JNK activation was detectable after 10 minutes and reached a plateau after 30 minutes exposure in this experimental condition (Fig. 3a). In corals, JNK activation was at its maximum within 30 minutes and these levels were maintained at least for up to 4 hours under stress condition (Fig. 3b). Hereby, we show that, like in human cells, JNK activation is involved in the early response of corals to stress.

Figure 3: Time course of JNK activation by combined UVR and thermal stresses in human fibroblasts and coral.
Figure 3

Immunoblot revealing activated (pJNK) and total JNK (JNK) in (a) human fibroblasts or (b) S. pistillata nubbins exposed to UVR and temperature rise for the indicated times. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of three independent experiments.

Respective contributions of UV and thermal stress to JNK activation

The effects of UV and temperature on early JNK activation were tested by subjecting fibroblasts and corals to the individual and combined stresses for 10 and 30 minutes, respectively. Ten minutes exposure to UVR alone had no significant effect on JNK activation in fibroblasts (Fig. 4a,c and Supplementary Table 1), although a longer exposure induced a strong activation (Supplementary Fig. S3). On the contrary, exposure to UVR triggered a detectable JNK phosphorylation in corals (p = 1.4 × 10−3) (Fig. 4b,d and Supplementary Table 1). Thermal stress strongly induced JNK activation in both models (p = 1.25 × 10−3 and p = 5.38 × 10−3 respectively). The combination of UV and thermal stress resulted in slightly higher but not statistically significant activation of JNK as compared to thermal stress alone (Fig. 4b,d and Supplementary Table 1). Treatment of cells and corals with the JNK inhibitor strongly reduced stress-induced JNK activation (p = 6.62 × 10−5 and p = 6.51 × 10−5 respectively). Notably, combining JNK inhibition to UVR stress had a dramatic effect on JNK protein stability in fibroblasts (Fig. 4a). We performed experiments to rule out the possibility that cell membrane disruption led to a leakage of the proteins out of the cells. First, we showed that the ROS carboxy-DCFDA probe used for ROS quantification did not leak out of the cells even after more than 30 minutes of exposure to a combination of UVR and SP600125 (Supplementary Fig. S4a). Moreover, the quantification of the staining of the cells with the non-permeant fluorescent dye propidium iodide (PI) showed no difference between the control and UV + SP conditions (Supplementary Fig. S4b). These results suggest that the disappearance of the JNK bands was not associated to cell membrane disruption. We then hypothesized that JNK degradation could be due to a cleavage by proteases. We first showed that JNK degradation is not caused by caspase cleavage as caspase inhibitor Z-VAD-FMK did not prevent the degradation of JNK protein in cells exposed to a combination of UV and SP600125 (Fig. S5). Moreover, western blot analysis of the PARP protein showed a cleavage of PARP in the UV + SP condition (Fig. S5). Interestingly, this cleavage was not inhibited by Z-VAD-FMK and resulted in a band of different size than the staurosporin-induced caspase-dependent one, suggesting that non-caspase proteases are activated in these conditions. To test a potential autophagy-driven degradation of JNK, we assessed the expression level of the SQSTM1 protein. We observed no increase in SQSTM1 expression level, suggesting that the UV + SP treatment did not activate autophagy (Fig. S5). Finally, treatment with the proteasome pharmacological inhibitor MG132 did not result in stabilization of JNK in the UV + SP condition, suggesting that JNK is not degraded by the proteasome either (Fig. S6).

Figure 4: JNK activation by UVR, temperature rise and combined UVR and temperature rise in human fibroblasts and coral.
Figure 4

Immunoblot revealing activated (pJNK) and total JNK (JNK) present in (a) human fibroblasts and (b) S. pistillata exposed to UVR (UV), increased temperature (T) or the combination of both stresses (UVT) in the absence (−) or presence (+) of SP600125. Total JNK and actin served as loading controls for human fibroblasts; total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of three independent experiments. Densitometric analysis of activated JNK intensities was performed on three independent blots for (c) human fibroblasts and (d) S. pistillata. The medians and standard deviations of three independent experiments are represented. Statistically significant differences, from the Tukey’s post hoc test are indicated with letters. Similar letters indicates no significant differences between conditions.

Inhibition of JNK in stress conditions impairs ROS scavenging capacities and results in strong adverse effects in corals and human cells

To gain more insight into the role of JNK activation in the early stress response, we assessed ROS levels in the experimental conditions described above. In both fibroblasts and corals, UVR induced a significant increase in ROS concentration (p < 2 × 10−16 and p = 1.78 × 10−10 respectively) (Fig. 5a,b and Supplementary Table 2). However, in both systems, thermal stress alone had no or very low effect on ROS content in the absence of JNK inhibitor (Fig. 5a,b and Supplementary Table 2). In corals, the inhibition of JNK activity leaded to an increase in ROS concentration in all conditions (p = 4.60 × 10−13) (Fig. 5b and Supplementary Table 2). A significant interaction between UVR and temperature led to a higher ROS concentration compared to each stress alone (p = 2.53 × 10−3) (Fig. 5b and Supplementary Table 2). In fibroblasts treated with JNK inhibitor, the ROS concentration did not change under high temperature but strongly increased under UVR with or without high temperature (p < 2 × 10–16) (Fig. 5a and Supplementary Table 2).

Figure 5: JNK activity protects corals and human cells from UV and temperature-induced oxidative stress and their adverse effects.
Figure 5

Staining intensity of carboxy-H2DCFDA (fluorescence at 488 nm), indicative of the presence of ROS, evaluated after 10 and 30 minutes respectively for (a) human fibroblasts and (b) S. pistillata with or without pre-incubation with SP 600125 in the following conditions: control (ct), UVR (UV), high temperature (T) and a combination of UVR and high temperature (UVT). The median, standard deviation and 95% confidence interval of at least three independent experiments are represented as boxplots. (c) Cell viability of human fibroblasts treated or not with SP600125 for 30 minutes prior to exposure to 10 minutes control (ct) or UVR (UV) measured 24 hours after the beginning of the experiment. (d) Symbiont content of S. pistillata nubbins treated or not with SP600125 for 1 hour prior to exposure to 4 hours control (ct) or combined UVR and thermal stresses (UVT) measured 24 hours after the beginning of the experiment. Medians and standard deviations are represented. Statistically significant differences, from the Tukey’s post hoc test are indicated with letters. Similar letters indicates no significant differences between conditions.

Next we studied the physiological consequences of increased ROS cellular concentrations in human fibroblasts and corals. Hence, we determined the fibroblast viability and the loss of symbiont (bleaching) in coral as read-outs of ROS-mediated stress. As combined thermal stress and JNK inhibition had no effect on ROS content in fibroblasts, we only assessed the UVR response. Exposure to UVR or to SP600125 alone did not affect the fibroblasts viability, while the mean cell survival upon exposure to UVR plus the JNK inhibitor was less than 10% (p = 1.07 × 10−3) (Fig. 5c and Supplementary Table 3). In corals, exposure to SP600125 or UVT alone did not induce bleaching. However, exposure to the combination of UVR and high temperature in the presence of the JNK inhibitor for 24 hours led to a loss of more than 30% of the symbionts (p = 1.21 × 10−2) (Fig. 5d and Supplementary Table 3). These results suggest that JNK activity is necessary for the organisms to control the advert effects triggered by the stress-induced ROS burst.

Discussion

While the physiological response of corals to UVR or thermal stress has been well studied27,28, the intracellular signalling that allows corals to rapidly respond to these stresses is still unknown. Our results strongly suggest the presence of a unique JNK protein in corals. This is in agreement with what was found in other cnidarians such as Nematostela vectensis or Hydra vulgaris49,50. For the first time, we showed a 100% conservation of the TPY motif in the catalytic domain of Spi and Hs-JNK. Phosphorylation of this domain by upstream kinases is essential for JNK activation. Hence, specific antibodies directed against the phosphorylated forms of Hs-JNK, which is an established player in the mammalian stress response15, have been generated to monitor Hs-JNK activation. The specificity of these antibodies was further demonstrated for corals, since, as in human cells, Spi-JNK was activated and/or inhibited by the same mammalian activator/inhibitor. In addition, a significant increase in JNK phosphorylation was observed under exposure to high temperature and/or UVR, suggesting that activation of this pathway is a common response of S. pistillata to various environmental disturbances. Although previous studies suggested that mRNA encoding members of the JNK signalling pathway could be up-regulated in the corals’ response to chronic stress conditions34,36,37, JNK protein activity, which is classically considered the most appropriate readout, especially in an acute response setting, has never been monitored in corals. Our study thus demonstrates that JNK activity in corals is stimulated by environmental conditions in a way comparable to mammalian cells4,51.

In S. pistillata, the phosphorylation of JNK was higher under thermal stress than UVR, and even seemed to be higher under the combination of both stressors, suggesting a cumulative effect of these two variables. This cellular response is also in agreement with the corals’ physiological response to the same conditions25,52,53 (Courtial et al. in press). Corals are usually well protected from UVR by a panel of natural sunscreens, such as mycosporine like amino acids54 whereas they are more sensitive to thermal stress alone or in combination with other stressors55. Phosphorylation of JNK under the same stresses was previously observed in two kelp algae, but their response was mostly driven by UVR11. Nonetheless, a cumulative effect of temperature and UVR on JNK activation was observed in algae, suggesting that JNK is a good proxy for stress in marine organisms. Its activation is also proportional to the intensity of the stress. Similar to corals and algae, increased phosphorylation of JNK was observed in human fibroblasts under thermal stress. Instead, the UV stress inflicted to human cells was below the threshold value necessary to enhance the basal JNK activity (Fig. 4 and Supplementary Fig. S3). Nonetheless, the combination of UVR and JNK inhibition significantly decreased fibroblast viability, suggesting that the basal JNK activity is necessary and sufficient to overcome a moderate UV stress. Moreover, we observed a decrease in total JNK amount in fibroblasts by combining UVR and JNK inhibition. Such rapid disappearance of JNK has never been described and was not associated to cell membrane disruption, to caspase cleavage, to autophagy processes or to proteasomal degradation. Although we don’t have a definitive explanation for such disappearance, the caspase-independent cleavage of PARP suggests that other proteases are activated in these conditions and may also cleave JNK56. In both corals and fibroblasts, JNK inhibition by SP600125 indeed resulted in ROS accumulation. This “binary result” strongly suggests that JNK plays a pivotal role in protecting the cells against ROS damages; however, based on our results, it is difficult to conclude whether JNK activation results from ROS production or from another mechanism. In fibroblasts, the inactivation of JNK by SP600125 during exposure to fairly low dose of UVR57,58 (3 W.m−2 UVB and 30 W.m−2 UVA for 10 minutes) led to the death of human cells. This observation supports the concept that, in the absence of active JNK, the fibroblasts cannot tolerate and neutralized the excessive production of UVR-induced ROS. This result contrasts with some studies showing that JNK activity is necessary for UVR-induced apoptosis14,59,60. However, our results are consistent with studies suggesting that JNK is also a key modulator of oxidative stress-mediated cell survival14,60,61,62. Discrepancies between studies may be explained by the use of different UV sources: whereas a UVA/B source that reproduces the UVR solar spectrum was used in our study, cells were exposed to UVC in the aforementioned studies14,59,60. In corals, the inactivation of the JNK pathway led to a slight bleaching (loss of symbiont cells) under the combined UVR/thermal stress, supporting the antioxidant function of the JNK signal transduction pathway. Consistent with our results, a protective role of JNK towards oxidative stress has also been reported in C. elegans and D. melanogaster63,64,65,66, suggesting that this function is one of the primary functions of JNK throughout the animal kingdom. In humans, JNK activation has been linked to the upregulation of Manganese dependent-Super Oxide Dismutase (Mn-SOD) and catalases depending on the deacetylase SIRT1 and the FOXO transcription factor13,64. In a more recent study, the early JNK response has been proposed to inhibit apoptosis67. Collectively, the results obtained in this study and other studies on various organisms suggest that the primary role of JNK signalling in thermal and solar spectrum UVR-induced oxidative stress consists of triggering a pro-survival anti-oxidant response. However, in case of a longer or more potent stress, e.g. exposure to UVC radiation59, JNK might play a role in the induction of apoptosis.

Overall, our results suggest that JNK activation may play an evolutionarily-conserved role from cnidarians to mammals in protecting cells from oxidative stress. As the intensity of JNK phosphorylation is directly related to the intensity of oxidative stress in corals, JNK activation may represent a good and rapid proxy and an easily detectable marker in field studies to predict the effects of environmental perturbations on corals. In a time of rapid climate change, the knowledge of cellular pathways involved in the response of corals to stress is particularly important. Our findings bring new insight into the mechanisms that corals employ to face environmental pressures and raise several questions about the functions of JNK in both coral and human cells.

Methods

Maintenance of coral nubbins and human fibroblasts

The scleractinian coral Stylophora pistillata (Pocilloporidae family) was chosen as a model because it has a broad geographic range (from the Red Sea to the western central Pacific68 and is widely used in laboratory experiments involving environmental stress effects, including short term thermal stress and UV exposure25. Colonies collected in the Gulf of Aqaba (Red Sea, Jordan) were cultured as previously described in Ferrier pages et al.25.

Skin fibroblasts (BJ-EHLT cells) were kindly provided by E. Gilson’s lab (IRCAN) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Invitrogen, Villebon-sur-Yvette, France) supplemented with 10% heat-inactivated fetal calf serum (FCS, Dutscher, Brumath, France) at 37 °C in an atmosphere of 5% CO2 as previously described69.

Antibody validation

The antibody directed against the activated JNK (pTPpY) was from Promega, Madison, Wisconsin (V7931); the pan-JNK antibody was from R&D Systems, Minneapolis, Minnesota (AF1387).

Coral nubbins were incubated in individual beakers filled with 40 mL of 0.45 μm filtered seawater and placed during one hour under the following conditions: 1) 0.005% DMSO (drug solvent), 2) 100 μmol.L−1 anisomycin, which is a JNK activator70 (Euromedex, Souffelweyersheim, France), 3) 40 μmol.L−1 of SP600125, which is a JNK inhibitor48 (Euromedex); 4) a condition in which nubbins were pre-incubated with SP600125 during one hour, then stimulated with anisomycin while kept in the presence of SP600125 for another hour. The incubation medium was continuously stirred using magnetic stirrers at a constant temperature of 25 °C and a photosynthetically active radiation of 200 μmoles photons m−2 s−1. At the end of the incubation, nubbins were frozen and kept at −80 °C prior to western blot analysis.

Skin fibroblasts (BJ-EHLT cells) were seeded at a density of 2 × 106 cells in 60 mm diameter dishes, and were subjected to the same treatments as the coral nubbins 24 hours later.

A Symbiodinium sp. culture (clade A1 isolated from S. pistillata,) in ASP8 medium71 at 25 °C and 150 μmoles photons m−2 s−1 was also used to check for the presence of the JNK protein. Samples were exposed to the same 4 conditions as the coral nubbins, pelleted by centrifugation at 14 g for 10 minutes, frozen and kept at −80 °C prior to western blot analysis.

Assessment of JNK activity in response to combined thermal and UV stress

High temperature and UVR stresses were applied to corals and human cells. For corals, the thermal stress corresponded to an increase in temperature from the normal culture condition of 25 °C to 30 °C. The UVR stress corresponded to an increase in UVR from 0 (HQI lamps in the culture conditions) to a radiation intensity of about 3 W.m−2 UVB and 30 W.m−2 UVA (Q-Panel UVA 340 lamps). These intensities correspond to those reaching the sea surface in tropical areas25. Similar dose were used in previous work on corals72,73,74 and in dermatological studies57,58. The UVR intensity was measured using ILT1400 portable radiometer and detectors (SEL240/UVB-1/TD and SEL033/UVA/TD) according to Shick et al.75. For human fibroblasts, thermal stress was induced by an increase in temperature from 37 °C to 42 °C, and UVR stress was the same as for coral nubbins.

JNK induction kinetics were evaluated on twelve coral nubbins, which were collected from the culture tanks and incubated during 4 h with constant agitation at 30 °C under UVR in beakers containing 40 mL of 0.45 μm filtered seawater. Three nubbins were frozen for each time point (0, 0.5, 1 and 4 hours). Fibroblasts were lyzed in Laemmli buffer prior to stress and after 5, 10 and 30 minutes incubations in the culture medium at 42 °C under UVR.

To assess JNK activity in response to individual and combined stress, coral nubbins were incubated in individual beakers, in the dark at 25 °C for 30 minutes in 40 mL of 0.45 μm filtered seawater containing 0.004% DMSO or 40 μmol.L−1 of JNK inhibitor (SP600125) prior to a 30 minutes exposure to the following conditions: 25 °C without UVR, 25 °C with UVR, 30 °C without UVR and 30 °C with UVR. Nubbins were flash frozen in liquid nitrogen and conserved at −80 °C prior to western blot analysis.

Similarly, BJ-EHLT fibroblasts were seeded at a density of 2 × 106 cells in 60 mm diameter dishes 24 h before the experiment. The cells were then incubated with 0.001% DMSO or 10 μmol.L−1 of SP600125 for 30 minutes at 37 °C prior to a 10 minutes exposure to the following conditions: 37 °C without UVR, 37 °C with UVR, 42 °C without UVR and 42 °C with UVR.

Western blot analysis

For coral samples, the tissue of each nubbin was removed from the skeleton using an air-pick in 1 mL of Laemmli buffer76. Samples were then sonicated for 30 seconds and centrifuged three times (5 minutes at 15 000 g) to remove the debris. Isolated Symbiodinium extracts were prepared from pellets re-suspended in 50 μL Laemmli buffer and then treated as the coral samples. Fibroblasts were washed twice in phosphate buffered saline (PBS), lyzed in Laemmli buffer directly in the dishes and sonicated for 30 seconds. For all samples, the total protein concentration was determined using a BCA protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s recommendation. 1,4 Dithiothreitol (1 mM)-bromophenol blue solution (0.1% bromophenol blue) was added to all samples and they were then heated for 5 minutes at 95 °C and 60 μg of total protein were separated on 10% polyacrylamide gels and transferred onto a PVDF membrane at an intensity of 200 mA at 4 °C overnight in DUNN buffer. Membranes were stained in a 20% 2-propanol - 10% acetic acid - 0.1% amido black solution, destained in 2 washes with a 20% 2-propanol - 10% acetic acid solution, rinsed in distilled water, blocked for 30 minutes in PBS - 3% low fat milk, rinsed in PBS and incubated with primary antibodies (anti-activated JNK: Promega, V7931; anti-pan-JNK: R&D Systems, AF1387; anti-beta Actin: Pierce MA5- 15739; anti-GAPDH: Pierce, MA5-15738; anti-SQSTM1/p62 Antibody: Cell Signaling Technology 5114) diluted in PBS - 1% low fat milk at 4 °C overnight. After extensive washing with PBS, membranes were incubated for 2 hours in the presence of the HRP-conjugated secondary antibody, rinsed in PBS and HRP activity was detected using Millipore ECL according to the manufacturer recommendations. An Odyssey device (LI-COR Biosciences, Lincoln, Nebraska) was used to image the membranes.

Densitometric analysis of the western blots was performed using the Image Studio software (Li-COR Biosciences). Intensity of the pJNK signal was normalised to the intensity of the loading controls.

ROS quantification

ROS detection was performed using the carboxy-H2DCFDA probe (Image-iT TM LIVE Green Reactive Oxygen Species Detection Kit (I36007), Molecular Probes Invitrogen. The apical tips of five coral branches were incubated in 750 μL of 0.45 μm filtered seawater containing 25 μmol.L−1 of carboxy-H2DCFDA probe in the presence of 0.001% DMSO or 10 μmol.L−1 SP600125 per condition. The apical tips were incubated for 30 minutes in the dark at 25 °C prior to exposure to the following conditions: 25 °C without UVR, 25 °C with UVR, 30 °C without UVR and 30 °C with UVR. Samples were then rinsed in filtered seawater and fluorescence was directly measured in the coral tissue with a Xenius SOF spectrofluorometer (SAFAS, Monaco) equipped with an optical fiber. The emission spectrum was measured between 515 and 550 nm with an excitation wavelength of 495 nm.

Human fibroblasts (0.5 × 106 in 35 mm dishes) were incubated for 30 minutes in culture medium containing 25 μmol.L−1 of carboxy-H2DCFDA probe in the presence of 0.001% DMSO or 10 μmol.L−1 SP600125 prior to exposure to the following conditions25 °C without UVR, 25 °C with UVR, 30 °C without UVR and 30 °C with UVR for 10 minutes. Cells were imaged using a LEICA DM4000 microscope with a 40x objective and a Zyla5.5 sCMOS camera (Andor Technology Ltd, Belfast, Northern Ireland). The software ImageJ77 was used to quantify the fluorescence of at least five fields from three independent experiments per condition.

Measurement of bleaching and cellular viability

Coral nubbins were treated for 1 hour with 5 μmol.L−1 SP600125 or 0.005% DMSO in standard culture conditions prior to exposure to UVR at 30 °C for 4 hours. Nubbins were then, rinsed, and placed back into control culture conditions for 24 hours. The symbiont content was measured as previously described78.

BJ-EHLT cells were seeded at a density of 106 cells in 60 mm diameter dishes the day before the experiment. The cells were then treated for 30 minutes with 1 μmol.L−0f SP600125 or with 0.001% DMSO in standard culture conditions, exposed to UVR for 10 minutes and washed 3 times with culture medium before being placed back in normal culture conditions. The cell viability was assessed 24 hours after the initial treatment using an ADAM automatic fluorescence cell counter (AlphaMetrix Biotech, Rödermark, Germany) following the manufacturer’s recommendations.

Statistical analysis

For ROS and western blot quantifications, conditions were compared using a three-way Analysis of Variance (ANOVA) with drugs (±SP 6000125), UVR (±UVR) and temperature (high or low temperature) as factors. The normality of the residuals and the homoscedasticity of the variances were tested using the Shapiro test and the Bartlett test, respectively. A Tukey’s Honest Significant Difference post hoc test was performed when significant differences were found. Fibroblast viability and the relative amounts of symbionts left at the end of the experiment were compared using a t-test. Normality of data was checked prior to running tests. For each test, we used 0.05 as alpha level. For western blot quantifications, the ANOVAs were ran on n = 3 replicates; and on n = 15 and n = 5 replicates per condition for ROS concentration in fibroblast and coral respectively. T-tests were performed on n = 3 and n = 4 replicates respectively.

Additional Information

How to cite this article: Courtial, L. et al. The c-Jun N-terminal kinase prevents oxidative stress induced by UV and thermal stresses in corals and human cells. Sci. Rep. 7, 45713; doi: 10.1038/srep45713 (2017).

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Acknowledgements

The authors thank D. Desgré for technical help during the experimental setup, Dr. Jeroen van de Water and Didier Zoccola for editorial assistance, Mrs Natacha Caminiti-Segonds for the first coral extracts and assays as well as Pr. Denis Allemand, Director of the Centre Scientifique de Monaco for scientific support. Financial support to L.C. was provided by the Centre Scientifique de Monaco and Paris VI University, GP was supported by the Fondation de France, the Association for Cancer Research, the National Institute of Cancer (InCA) and “Cordon de vie, Monaco”.

Author information

Author notes

    • Lucile Courtial
    •  & Vincent Picco

    These authors contributed equally to this work.

Affiliations

  1. Centre Scientifique de Monaco, Marine Department, 8 Quai Antoine Ier, MC-98000 Monaco, Principality of Monaco

    • Lucile Courtial
    • , Renaud Grover
    • , Cécile Rottier
    • , Antoine Labbe
    •  & Christine Ferrier-Pagès
  2. Sorbonne Universités, UPMC Univ Paris 06 IFD-ED 129, 4 Place Jussieu, 75252 PARIS cedex 05, France

    • Lucile Courtial
  3. UMR ENTROPIE (IRD, Université de La Réunion, CNRS), Laboratoire d’Excellence « CORAIL», BP A5, 98848, Nouméa cedex, New Caledonia

    • Lucile Courtial
  4. Centre Scientifique de Monaco, Biomedical Department, 8 Quai Antoine Ier, MC-98000 Monaco, Principality of Monaco

    • Vincent Picco
    • , Yann Cormerais
    •  & Gilles Pagès
  5. University Nice Sophia-Antipolis, Institute for Research on Cancer and Aging of Nice (IRCAN), CNRS UMR7284/INSERM U1081, Centre Antoine Lacassagne, Nice 06189, France

    • Gilles Pagès

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Contributions

Conceived and designed the experiments: C.F.P., G.P., L.C., V.P., Y.C. Performed the experiments: L.A., L.C., R.C., R.G., V.P., Y.C. Analysed the data: C.F.P., G.P., L.C., V.P. Contributed reagents/materials/analysis tools: C.F.P., G.P. Wrote the paper: C.F.P., G.P., L.C., V.P.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Gilles Pagès or Christine Ferrier-Pagès.

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