Abstract
SRY, a sex-determining gene, induces testis development in chromosomally female (XX) individuals. However, mouse XX Sertoli cells carrying Sry (XX/Sry Sertoli cells) are incapable of fully supporting germ cell development, even when the karyotype of the germ cells is XY. While it has therefore been assumed that XX/Sry Sertoli cells are not functionally equivalent to XY Sertoli cells, it has remained unclear which specific functions are affected. To elucidate the functional difference, we compared the gene expression of XY and XX/Sry Sertoli cells. Lactate and cholesterol metabolisms, essential for nursing the developing germ cells, were down-regulated in XX/Sry cells, which appears to be caused at least in part by the differential expression of histone modification enzymes SMCX/SMCY (H3K4me3 demethylase) and UTX/UTY (H3K27me3 demethylase) encoded by the sex chromosomes. We suggest that down-regulation of lactate and cholesterol metabolism that may be due to altered epigenetic modification affects the nursing functions of XX/Sry Sertoli cells.
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Introduction
In mammals, the SRY gene (the sex determining region on the Y chromosome) has generally been thought to be sufficient for differentiation of the testes1,2,3. Indeed, an Sry transgene successfully induced testis development in XX fetuses; testicular cords were organized, Sertoli cells were differentiated within the cords, and Leydig cells were present in the interstitial space4. However, XX mice carrying an Sry transgene (XX/Sry) were found to be infertile5,6. Phenotypically, spermatogonial cells disappear from the testes soon after birth, and the presence of double X chromosomes has been suggested as a cause7. Moreover, since genes essential for spermatogenesis are localized on the Y chromosome8, XX germ cells are incapable of differentiating into matured male germ cells. The infertility of XX/Sry males has therefore been discussed from the viewpoint of a functional deficit of germ cells. It has, however, remained largely unclear whether XX/Sry Sertoli cells exhibit functions equivalent to XY Sertoli cells. Ishii et al.6 reported the interesting experimental observation that XY germ cells implanted into XX/Sry testes differentiated into round spermatids but rarely elongated spermatids. The authors concluded that the milieu established by XX/Sry Sertoli cells is insufficient for differentiation into elongated spermatids. However, the specific functions that have been affected in XX/Sry Sertoli cells still await clarification.
Since blood vessels are localized in the interstitial space outside the seminiferous tubules and Sertoli cells create a tight blood-testis barrier, nutrients and fuels for energy production cannot be supplied to germ cells via the blood. The Sertoli cells, often referred to as nursing cells, are responsible for the supply of energy and nutrients to the germ cells, with which they remain in close contact throughout the entire differentiation process9. Similar to nutrients, oxygen supply is restricted in the seminiferous tubule, and the testis has therefore been described as an oxygen-deprived organ10. In this unusual milieu, spermatocytes and mature sperms prefer lactate as fuel to produce ATP11. Sertoli cells produce lactate via glycolysis and then supply it to developing germ cells12,13.
Another fundamental material supplied to germ cells by Sertoli cells is cholesterol14. Sertoli cells are capable of synthesizing cholesterol by themselves, as well as absorbing it from high density lipoprotein (HDL)15,16. They also continuously phagocytose developing germ cells as another source of cholesterol17. Consequently, the quantity of intracellular cholesterol/cholesterol ester is regulated by the balance of synthesis, influx via the two above-mentioned routes, and efflux. It has been suggested that ATP-binding cassette transporter 1 (ABCA1) mediates cholesterol efflux from Sertoli cells, since disruption of Abca1 gene led to defects in spermatogenesis together with unusual accumulation of lipids in the Sertoli cells18. In addition, gene knockout of retinoid X receptor β (Rxrb, Nr2b2)19 and double knockout of liver X receptor α/β (Lxrα, Nr1h3 and Lxrβ, Nr1h2)20 resulted in defects similar to Abca1 gene knockout, possibly through down-regulation of Abca1 gene transcription.
Sex chromosomes carry genes encoding histone modification enzymes such as SMCX (KDM5C)/SMXY (KDM5D) and UTX (KDM6A)/UTY. Both SMCX and SMCY mediate the demethylation of histone H3 trimethylated Lysine 4 (H3K4me3)21. UTX mediates the demethylation of histone 3 trimethylated Lysine 27 (H3K27me3), whereas such activity has not been found for UTY22,23. Evidence from multiple sources indicates that H3K4me3 accumulates predominantly around the transcription start sites of active genes, while H3K27me3 is distributed throughout gene bodies with inactive transcription24,25,26. The physiological function of Utx has been investigated using gene knockout mice27,28,29. Interestingly, in addition to affecting morphology, Utx was found to be required for sexually dimorphic deposits of H3K27me329.
In the present study, we investigated the functional differences between XY and XX/Sry Sertoli cells by focusing on their role as nursing cells.
Results
Preparation of XY and XX/Sry Sertoli cells
To examine the contribution of sex chromosomes to gene expression in Sertoli cells, we used XY wild type and XX transgenic mice carrying the Sry transgene (XX/Sry). Sertoli cells from these mice were labeled with EGFP as described in ‘Materials and Methods’. As expected, all SOX9-positive (SRY-box containing gene 9) Sertoli cells were positive for EGFP in the testes of XX/Sry as well as XY wild type mice on postnatal days 1 and 21 (Fig. 1a). As reported previously5, germ cells had disappeared from the seminiferous tubules of the XX/Sry testes by P21. Whole testicular cells prepared from P1 and P21 testes were subjected to FACS. EGFP-positive and -negative cell fractions were recovered (Fig. 1b). Fluorescence microscopy indicated that more than 92% of the cells were EGFP-positive in all preparations (Fig. 1c).
Total RNAs were prepared from P1 and P21 EGFP-positive and -negative cells at and used for qRT-PCR analysis (Fig. 1d). As expected, Sox9 mRNA was enriched in the EGFP-positive cell fractions, whereas a germ cell marker (homologue of a DEAD (Asp-Glu-Ala-Asp) family gene (Ddx4, VASA)), and a Leydig cell marker (Hsd3b1 (Hydroxysteroid dehydrogenase Type 3b1)) were enriched in the EGFP-negative cell fractions. Consistent with the disappearance of germ cells from XX/Sry testes, expression of Ddx4 was much reduced in the EGFP-negative cell fraction of XX/Sry testes. Up-regulation of Hsd3b1 in these cells might have resulted from an increased proportion of Leydig cells following the disappearance of the germ cells. Taken together, these marker gene expressions indicate that the EGFP-positive cell fractions prepared from the XY and XX/Sry testes comprised predominantly Sertoli cells.
Hormones necessary for reproductive activities were measured in XY and XX/Sry mice at P21. As shown in Fig. 1e, plasma testosterone was decreased in XX/Sry mice as compared to XY mice. Such significant alteration was not observed in the amounts of follicle stimulating hormone (FSH) or luteinizing hormone (LH). Consistent with the decreased testosterone concentration, the testicular size of XX/Sry mice was smaller than that of XY mice (Fig. 1f). Estradiol is a potent estrogen in females, but the concentration is too low to determine precisely in males. Therefore, we determined the expression level of the Cyp19 gene, which is essential for the synthesis of estradiol. As shown in Fig. 1g, the expression of Cyp19 in the XX ovary was higher than that in the XY testis. Cyp19 gene expression was not observed in the XX/Sry testes, suggesting that estradiol could not be synthesized in the XX/Sry testis.
Gene expression in XY and XX/Sry Sertoli cells
The RNAs prepared from XY and XX/Sry Sertoli cells at P1 and P21 were sequenced. As summarized in Supplemental Table S1, approximately 30 million reads were obtained from every sample. More than 97% of reads were mapped to the reference genome, suggesting that the sequence data sets were of sufficient quality for further analyses. Gene expressions were compared between the two types of Sertoli cells at P1 and P21. Correlation coefficients between the cell types were 0.997 at P1 and 0.971 at P21, indicating that the gene expressions of the two types of Sertoli cells were very similar at P1 and differed slightly more at P21 (Fig. 2a). Consistent with this, 38 genes were up-regulated more than 1.5-fold and 86 genes down-regulated less than 1.5-fold in the P1 XX/Sry Sertoli cells, and 422 and 834 genes were respectively up- and down-regulated by the same margins in the P21 XX/Sry Sertoli cells (Fig. 2b).
Genes displaying differential expression are listed in Supplemental Tables S2–S5. Sry and Xist (inactive X-specific transcript) were treated as up-regulated genes in the P1 XX/Sry Sertoli cells (Supplemental Table S2). In the case of Sry, this was because the expression of the exogenous Sry gene is driven by Hsp70.3 basal promoter. The increase of Xist suggests that X chromosome inactivation occurs even though the fate of cells carrying two X chromosomes is changed to male supporting Sertoli cells. Four Y-linked genes (Smcy (Kdm5d), Ddx3y, Eif2s3y, and Uba1y) were recorded as down-regulated in P1 XX/Sry Sertoli cells (Supplemental Table S3). This is consistent with the fact that the Y chromosome is absent from XX/Sry transgenic mice. Interestingly, the expression of genes for ribosomal protein (Rpl36, Rps29, Rplp1, and Rps21) and mitochondrial ribosomal protein (Mrps12) was decreased in the XX/Sry Sertoli cells.
Genes that were up- or down-regulated in P21 XX/Sry Sertoli cells are summarized in Supplemental Tables S4 and S5. We attempted to extract the biological events/pathways related to the listed genes by conducting GO and KEGG pathway analyses. Results are summarized in Supplemental Tables S6 and S7.
Genes up-regulated in P21 XX/Sry Sertoli cells were not distinguished by high fold enrichment and P-value (Supplemental Table S6), whereas this was not the case for down-regulated genes (Supplemental Table S7). Differences in the expression of genes with functions related to lactate metabolism and sterol/terpenoid metabolism were particularly noticeable and are discussed in the following sections.
Lactate production decreased in XX/Sry Sertoli cells
It has been established that lactate supplied by Sertoli cells is utilized as an energy source by developing germ cells such as spermatocytes, spermatids, and spermatozoa13. Interestingly, genes related to ‘lactate dehydrogenase activity’ were among those down-regulated in P21 XX/Sry Sertoli cells. Lactate dehydrogenase (LDH) is a tetrameric enzyme of lactate dehydrogenase A (LDHA) and B (LDHB) subunits encoded by Ldha and Ldhb, respectively. Sequencing indicated that expression of Ldha was decreased in P21 XX/Sry Sertoli cells, while that of Ldhb was slightly increased (Fig. 3a). qRT-PCR analysis confirmed the sequence data (Fig. 3b). As expected, LDHA protein was decreased in P21 XX/Sry Sertoli cells (Fig. 3c).
LDH with four subunits of LDHA is known to preferentially mediate conversion from pyruvate to lactate. By contrast, LDH with four subunits of LDHB mediates conversion from lactate to pyruvate30,31. In addition to these, three distinct isoenzymes (one A/three B, two A/two B, and three A/one B) can be formed with LDHA and LDHB, and are thought to exhibit an intermediate level of activity32. Considering the decreased expression of Ldha and increased expression of Ldhb in the XX/Sry Sertoli cells, lactate production may be lower in these cells.
In addition to synthesis, the lactate transport activity of Sertoli cells should be considered. Monocarboxylate transporters encoded by Mct1 (Slc16a1), Mct2 (Slc16a7), Mct3 (Slc16a8), and Mct4 (Slc16a3) have been identified as lactate transporters. Mct1 and Mct4 were found to be expressed in the Sertoli cells, whereas Mct2 and Mct3 were mostly absent (Fig. 3a). Sequencing and qRT-PCR analysis indicated that expression of Mct1 and Mct4 was decreased slightly and strongly, respectively, in XX/Sry Sertoli cells (Fig. 3a,b).
MCT1 and MCT4 are responsible for the import and export of lactate, respectively33,34,35. Considering the down-regulated expression of both Mct4 and lactate-synthesizing Ldha, the quantity of lactate efflux from XX/Sry Sertoli cells was expected to be lower. To investigate this, Sertoli cells from XY and XX/Sry testes at P21 were cultured and the quantities of lactate in the culture media determined. Lactate in the media of both types of Sertoli cells was found to increase over time (Fig. 3c). As expected, amounts were significantly higher in XY than in XX/Sry Sertoli cells, possibly indicating that the activity of lactate supply to germ cells is strongly impacted in XX/Sry Sertoli cells.
Cholesterol production decreased in XX/Sry Sertoli cells
Sertoli cells supply cholesterol to germ cells. The metabolism, influx, and efflux of cholesterol in or from Sertoli cells are therefore critical for germ cell development. Biological functions related to cholesterol and sterol metabolism were found to be associated with genes that were down-regulated in P21 XX/Sry Sertoli cells. In fact, sequence data indicated that the expression of many cholesterogenic genes was down-regulated (Fig. 4a). Similarly, qRT-PCR analysis showed that the expression of 13 out of 20 cholesterogenic genes was significantly decreased in XX/Sry Sertoli cells (Fig. 4b). Consistent with the results above, immunoblot studies revealed the amount of CYP51 protein decreased in XX/Sry Sertoli cells. Unexpectedly, however, the amount of HMGCR was unchanged (Fig. 4c). Since the amount of HMGCR is regulated post-translationally36, HMGCR might be stabilized in XX/Sry Sertoli cells.
SREBP2 (sterol regulatory element binding protein 2), also known as SREBF2, has been established as the master regulator of cholesterogenic gene transcription37,38. Our results obtained by sequencing (Fig. 4d) and qRT-PCR analysis (Fig. 4e) showed a significantly decreased expression of Srebf2 but unaffected expressions of Sox9, Ad4BP/SF-1, Dmrt1, Amh, and Dhh in XX/Sry Sertoli cells. We consequently investigated whether the decreased expression of Srebf2 led to a decrease in cholesterogenic gene expression in XY Sertoli cells. siRNA treatment successfully decreased the expression of Srebf2 (Fig. 4f). qRT-PCR of cholesterogenic genes revealed that eight genes were suppressed by the treatment (Fig. 4g). Seven of these (Hmgcr, Idi1, Sqle, Cyp51, Msmo1, Hsd17b7, and Dhcr24) were among the genes down-regulated in XX/Sry Sertoli cells, suggesting that the down-regulation of the cholesterogenic genes was primarily the result of the decreased expression of Srebf2.
Influx and efflux as well as synthesis should be considered in cholesterol homeostasis. Influx of cholesterol into Sertoli cells is predominantly mediated by HDL receptor/SRB1 encoded by Scarb1, while efflux is mediated by ABCA1 encoded by Abca116,39. Sequencing and qRT-PCR analysis indicated that the expression of Scarb1 was not affected and that Abca1 expression was unlikely to be down-regulated in XX/Sry Sertoli cells (Fig. 4d,e).
Since these results strongly suggested that cholesterol synthesis is affected in XX/Sry Sertoli cells, we investigated cholesterogenic activity. The amount of 14C-labeled free cholesterol in cultured XX/Sry Sertoli cells was 60% of that in XY Sertoli cells (Fig. 5a). We also determined the quantities of cholesterol together with lanosterol, lathosterol, and desmosterol, all of which are intermediate molecules in the cholesterogenic pathway (Fig. 5b). As expected, the amounts of lathosterol and desmosterol were substantially smaller in XX/Sry Sertoli cells (Fig. 5c). Unexpectedly, this was not the case for cholesterol. This may be because the germ cells had mostly disappeared from the XX/Sry testes by P21 and the Sertoli cells had thus lost the cells to which they would have transferred their cholesterol.
Differential epigenetic regulation in XY and XX/Sry Sertoli cells
Since Uty and Smcy are localized on the Y chromosome, transcripts of these genes were undetectable in XX/Sry Sertoli cells by either sequencing or qRT-PCR (Fig. 6a,b). The expression of Utx and Smcx was roughly consistent with the gene dosage (a single copy in XY and two copies in XX/Sry Sertoli cells). This dosage-dependent expression is consistent with the observation that these genes escape from X chromosome inactivation40,41. Interestingly, the FPKM value of Smcx was 10-fold higher than that of Smcy, suggesting that the demethylation activity of H3K4me3 was stronger in XX/Sry than in XY Sertoli cells, assuming that its protein products could mediate demethylation with similar enzyme specific activity.
These differential expressions of histone modification enzymes raise the possibility that the methylation status of H3K4 and H3K27 was different in the two types of Sertoli cells. To examine this, we performed genome-wide ChIP-sequencing of both types of Sertoli cells at P21 using the antibodies for H3K4me3 and H3K27me3. H3K4me3 was found to be accumulated around the TSS of genes (Fig. 6c). As expected, the accumulation was substantially lower in XX/Sry Sertoli cells, possibly due to higher expression of Smcx. The accumulation of H3K27me3 distributed along the gene body was slightly higher in XX/Sry than XY Sertoli cells.
We then examined whether the differential status of active H3K4me3 and suppressive H3K27me3 was relevant to the differential gene expression in XY and XX/Sry Sertoli cells. As expected, the accumulation of H3K4me3 was greater at the TSS of genes that were up-regulated in XX/Sry Sertoli cells (p < 0.01), while accumulation was lower around down-regulated genes (p < 0.001; Fig. 6d). Accumulation of H3K27me3 was greater around genes that were down-regulated in XX/Sry Sertoli cells (p < 0.01), while there was no significant accumulation around up-regulated genes (p = 0.268).
The expression of Ldha and Mct4, implicated in the supply of lactate to germ cells, was down-regulated in XX/Sry Sertoli cells as described above (Fig. 3b). Consistent with this finding, the accumulation of active H3K4me3 and suppressive H3K27me3 was smaller and greater, respectively, at both Ldha and Mct4 gene loci (Fig. 6e). As noted above, the expression of 13 genes involved in cholesterogenesis was down-regulated in XX/Sry Sertoli cells (Fig. 4b). H3K4me3 was decreased to varying degrees around most cholesterogenic genes except Acta2, Mvd, and Fdft1. An increased tendency for H3K27me3 accumulation was observed in more than half of the cholesterogenic genes.
These histone modifications (decreased H3K4me3 and increased H3K27me3) probably lead to the down-regulation of gene expression. In fact, among the 13 cholesterogenic genes down-regulated in XX/Sry Sertoli cells, such changes were observable in Hmgcr, Mvk, Fdps, Sqle, Lss, Nsdhl, Sc5d, and Dhcr24. As described above, we examined which genes were affected by the down-regulation of Srebf2 in XX/Sry Sertoli cells, and to our surprise found that the expression of Mvk, Mvd, Fdps, Lss, Nsdhl, and Scd5d was not subject to down-regulation as a result of Srebf2 knockdown. The chromatin state of all these gene loci (with the exception of Mvd) was changed from active to inactive as a result of the decrease in H3K4me3 and increase in H3K27me3. These epigenetic changes might induce the down-regulation of gene expression independently of SREBF2 function in XX/Sry Sertoli cells. It should also be noted that the accumulation of H3K4me3 was lower in Srebf2, possibly causing the down-regulation of cholesterogenic genes.
Discussion
To achieve a better understanding of the functional differences between XY and XX/Sry Sertoli cells, we compared the mRNA expression profiles of the two cell types and determined which gene expressions were up- or down-regulated in XX/Sry Sertoli cells. GO and KEGG pathway analyses suggested that lactate and cholesterol metabolisms are impaired in XX/Sry Sertoli cells. Considering that the function of Sertoli cells is the nursing of developing germ cells, these effects on metabolic pathways are intriguing.
The energy metabolic pathway functioning in male germ cells is known to change during the course of differentiation from spermatogonia to spermatozoa11. Spermatogonia use glucose as fuel for ATP production, spermatocytes begin to utilize lactate, and later-stage germ cells such as spermatids and spermatozoa are highly depend on lactate as an energy source12,13. The carbohydrate metabolism of Sertoli cells is unusual; only 25% of the pyruvate produced by glycolysis is oxidized by the TCA cycle42, and cultured Sertoli cells mediate reactions from glucose to lactate via pyruvate43. This lactate is thought to be supplied to the developing germ cells.
The present study demonstrates that the expression of genes related to lactate metabolism differs between XY and XX/Sry Sertoli cells. Quantitative studies have found that Ldha is down-regulated in XX/Sry Sertoli cells, suggesting the possibility that lactate supply to developing germ cells is reduced in XX/Sry testes. This might be supported by the transplantation study of XY germ cells into XX/Sry testes performed by Ishii et al.6. Although the transplanted XY germ cells were capable of completing meiosis, their differentiation into elongated spermatids was impaired in the milieu established by the XX/Sry Sertoli cells. Considering that the predominant energy fuel shifts from glucose to lactate at the spermatocyte or spermatozoon stage, it can be assumed that a lower supply of lactate impedes the differentiation of the spermatocyte into an elongated spermatid.
Another crucial nursing function of Sertoli cells is thought to be the supply of cholesterol to germ cells14. Cholesterol homeostasis in Sertoli cells is preserved in several ways, such as de novo synthesis, influx via the HDL receptor, and efflux via the ABCA1 transporter. Perturbation of cholesterol homeostasis by disruption of Abca1 resulted in significantly affected spermatogenesis and fertility, in addition to abnormal lipid accumulation in the Sertoli cells18.
Our study demonstrated that expression of 13 of the 20 cholesterogenic genes was significantly down-regulated in XX/Sry Sertoli cells. Probably because of this suppressed gene expression, de novo cholesterol synthetic activity was lower in XX/Sry than in XY Sertoli cells. Expression of Abca1 and Scarb1 (involved in influx and efflux of cholesterol) was not markedly affected, suggesting that cholesterol transfer remains normal in XX/Sry Sertoli cells and that they consequently should feature lower quantities of intracellular cholesterol. However, no difference in the amount of free and esterified cholesterol in the two Sertoli cell types was found. While we are unable to suggest a plausible explanation for this apparent contradiction, the disappearance of germ cells from XX/Sry testes might affect the functioning of the XX/Sry Sertoli cells by removing the targets of their cholesterol transfer, thus preventing a decrease in their cholesterol content.
Determining the causes of the differential expression of metabolic genes in XX/Sry Sertoli cells is an important aim of this research. The cholesterogenic gene Srebf2, encoding an already-known key factor for cholesterogenic gene regulation37,38, was found to be down-regulated in XX/Sry Sertoli cells, suggesting a knockdown experiment to determine whether down-regulated Srebf2 results in reduced expression of cholesterogenic genes. This experiment confirmed the decreased expression of seven of the 13 genes whose expression was down-regulated in XX/Sry Sertoli cells. It was therefore assumed that the remaining six genes were regulated by a mechanism independent of SREBF2.
A candidate for the regulation mechanism was suggested by the fact that the genes encoding histone modification enzymes are localized on the sex chromosomes. SMCX on the X and SMCY on the Y chromosome mediate the demethylation of an active histone mark, H3K4me321. UTX mediates the demethylation of a suppressive histone mark, H3K27me3, whereas UTY does not exhibit this activity22,23. Because of the differential expression of these genes in XY and XX/Sry Sertoli cells, it was assumed that the methylation statuses of H3K4 and H3K27 were affected accordingly. Importantly, these epigenetic changes occurred in many, if not all, of the cholesterogenic genes, and we therefore suspect that they cause the down-regulation of cholesterogenic gene expression in XX/Sry Sertoli cells.
These observations may support the idea that Smcx/Smcy and Utx/Uty, which are localized on the sex chromosomes, regulate sexually dimorphic gene expression. Indeed, a Utx gene knockout study demonstrated that UTX regulates the level of H3K27me3, suggesting that the difference in Utx gene dosage between the two sexes leads to sex-dependent deposition of H3K27me329. Sex-dependent differences in deposition of H3K27me3 were also identified in hepatocytes and primordial germ cells44,45.
Gene expression in Sertoli cells is regulated by the testosterone and androgen receptor, AR (NR3C4)46. Upon ligand binding, AR forms protein complexes with histone modification enzymes, thereby changing chromatin structure to regulate target gene expression47,48. Plasma testosterone in XX/Sry mice was found to be lower than that in XY males. Although testosterone did not completely disappear from XX/Sry mice, this decrease may lead to a suppression of AR target gene expression. Because it is unclear whether the genes involved in lactate and cholesterol metabolism are targets of AR, we cannot exclude the possibility that the decreased expression of those genes is due to the decreased testosterone. Identification of AR target genes in Sertoli cells would be needed to resolve this issue.
In summary, we examined the functional differences between XY and XX/Sry Sertoli cells and demonstrated that lactate and cholesterol metabolism, both of which play a crucial role in the nursing of developing germ cells, are down-regulated in XX/Sry Sertoli cells. Moreover, our results suggest that these differential functions are at least in part the result of differential expression of histone modification enzymes encoded by sex chromosomes. Although it is well known that XX testes cannot support the differentiation of germ cells even if they carry XY chromosomes, the reason for this has remained unclear. This study suggests that this phenomenon may be caused by the down-regulation of lactate and cholesterol metabolism resulting from altered epigenetic modification.
Methods
Mice
A line of XX sex-reversed mice was established using an Sry transgene driven by the basal promoter of the Hsp70.3 gene49. The presence of the transgene in mice was confirmed by PCR with primers for Sry (Supplemental Table S8). Genetic sex (XY or XX) was determined by PCR using the primers for Ube1. Sox9-EGFP knock-in mice have been reported previously50. To label XX/Sry Sertoli cells with EGFP, XX/Sry mice were mated with Sox9-EGFP mice. All protocols for the animal experiments were approved by the Animal Care and Use Committee of Kyushu University. All experiments were performed in accordance with the guidelines.
Immunofluorescence microscopy
Frozen sections prepared from testes were used for immunostaining51. Anti-EGFP rat monoclonal antibody (1:1000; Nacalai Tesque, Kyoto, Japan) and anti-SOX9 rabbit antiserum52 (1:2000) were used as the primary antibodies, and ALEXA Fluor 488 goat anti-rat IgG and ALEXA Fluor 555 goat anti-rabbit IgG (1:500; Thermo Fisher Scientific, Waltham, MA, USA) were used as the secondary antibodies. DAPI (4′6′-diamidino-2-phenylindole) was used for nuclear staining. The specimens were observed using a BZ-9000 microscope (Keyence, Osaka, Japan).
Preparation and culture of Sertoli cells
Testes at postnatal days 1 and 21 (P1 and P21) were incubated in Earle’s balanced salt solution (Sigma, St. Louis, MO, USA) containing 1 mg/ml collagenase (Thermo Fisher Scientific), 0.5 mg/ml dispase (Thermo Fisher Scientific) and 2.5 mg/ml trypsin (Sigma) at 34 °C for 30 min, and following the addition of 0.3 mg/ml deoxyribonuclease I (Roche, Basel, Switzerland), were incubated for another 30 min at 34 °C. After centrifugation, the cells were suspended in PBS with 7-amino-actinomycin D (7AAD; BD Bioscience, Franklin Lakes, NJ, USA). The cells were washed with PBS containing 0.3 mg/ml deoxyribonuclease I, and filtered using a 70 μm cell strainer (BD Bioscience). EGFP-positive Sertoli cells were isolated by fluorescence-activated cell sorting (FACS) using a JSAN cell sorter (Bay bioscience, Kobe, Japan). For siRNA treatment, Sertoli cells at P21 were cultured at 34 °C on 24-well culture plates (Asahi Glass, Tokyo, Japan) pre-coated with collagen type I (Cell Matrix I-C; Nitta Gelatin, Osaka, Japan) in DMEM/Ham’s F-12 (1:1; Nacalai Tesque) supplemented with 10% FBS and Penicillin-streptomycin-glutamine (Thermo Fisher Scientific).
siRNA treatment
Using an RNeasy Mini or Micro kit (Qiagen, Hilden, Germany), total RNA was prepared from XY and XX/Sry Sertoli cells, and XY Sertoli cells were treated with siRNA duplex (stealth RNAi™; Srebf2-MSS277288 or Negative Control Medium GC Duplex; Thermo Fisher Scientific) using Lipofectamine RNAiMAX reagent (Thermo Fisher Scientific) for 48 h.
Quantitative RT-PCR (qRT-PCR)
Total RNAs were prepared from Sertoli cells at P1 and P21, and from testes and ovaries at P21. They were subjected to cDNA synthesis using M-MLV reverse transcriptase (Thermo Fisher Scientific), and then to qRT-PCR using SYBR Select Master Mix (Thermo Fisher Scientific) and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA). The primers used for qRT-PCR are listed in Supplemental Table S8. Listed values were standardized using β-actin (Actb) or 18 s ribosomal RNA (Rn18s). RT-PCR was performed in biological triplicate. Data are presented as means ± 1 standard deviation (SD). Differences between experimental groups were tested for significance using a two-tailed Student’s t-test.
mRNA sequencing and data analyses
Poly(A)+ RNAs were prepared from XY and XX/Sry Sertoli cells using oligo (dT) magnetic beads. Preparation of mRNA-seq library and subsequent sequencing was carried out as described previously53. Mapping and quantification of gene expression was performed by Tophat54, version 2.0.8, and RSEM55, version 1.2.11, respectively. Expression levels of genes were represented using the number of fragments per kilobase of transcript per million fragments mapped56 (FPKM). Fold change in FPKM values in XX/Sry Sertoli cells relative to XY Sertoli cells was calculated. Gene sets were subjected to gene ontology57 (GO) and KEGG pathway analyses using DAVID58. mRNA-seq data have been deposited in DDBJ/EMBL/GeneBank under accession code DRA004090.
Immunoblot analysis
Whole cell lysates were prepared from XY and XX/Sry Sertoli cells using lysis buffer (50 mM Tris-HCl pH 8.0, 50 mM NaCl, 1 mM EDTA, and 1% Sodium Dodecyl Sulfate (SDS)). After the protein concentration was determined using a BCA™ Protein Assay Kit (Pierce Biotechnology, Rockford, IL), 10 μg of the whole cell lysates were subjected to SDS-polyacrylamide gel electrophoresis, followed by immunoblotting. Anti-LDHA (Cell Signaling Technology, Danvers, MA, 1:1000), anti-HMGCR (Abcam, Cambridge, MA, 1:1000), anti-CYP51 (1:1000)59, or anti-α tubulin antibody (T-9026, Sigma-Aldrich, St. Louis, MO, 1:1000) was used as the primary antibodies. Anti-rabbit donkey IgG (1:1000) and anti-mouse donkey IgG (GE Healthcare, Piscataway, NJ, 1:1000) were used as the secondary antibodies. Bound antibodies were detected using the Chemi-Lumi One L Western Blotting Detection System (Nacalai Tesque, Kyoto, Japan).
Chromatin immunoprecipitation-sequence (ChIP-seq)
A total of 106 Sertoli cells fixed by formaldehyde (0.5%, 5 min at room temperature) were lysed with 600 μl Lysis Buffer (5 mM HEPES (pH 8.0), 200 mM KCl, 1 mM CaCl2, 1.5 mM MgCl2, 5% Sucrose, 0.5% Triton X-100), and then sonicated (5 × 15-s pulses with 59-s break intervals) using the Bioruptor plus sonication device (Diagenode, Denville, NJ, USA). Samples were then digested with 100 U/ml micrococcal nuclease (MNase; Takara Bio, Shiga, Japan) at 37 °C for 1 h to shear the chromatin. MNase digestion was terminated by the addition of 5 mM EDTA (pH 8.0). The sheared chromatin fraction was centrifuged at 15,000 × g for 10 min to remove insoluble materials. Supernatant was then incubated overnight at 4 °C with magnetic beads (Dynabeads Protein A; Veritas, Tokyo, Japan) pre-bound with a mouse monoclonal antibody against H3K4me3 or H3K27me3. The beads were washed with Lysis Buffer, Wash Buffer 2 (5 mM HEPES (pH 8.0), 500 mM KCl, 1 mM CaCl2, 5% Sucrose, 0.5% NP-40), and Wash Buffer 3 (10 mM Tris-HCl (pH 8.0), 1 mM EDTA (pH 8.0)). Finally, chromatin fractions (ChIP fractions) were eluted from the beads with 50 mM Tris-HCl (pH 8.0), 10 mM EDTA (pH 8.0), and 1% SDS. After crosslinking was reverted by heating at 65 °C for 16 h, DNA fragments were purified using QIAquick PCR Purification kit (Qiagen), and used to prepare a ChIP-seq library with TruSeq ChIP Sample Preparation Kit (Illumina, San Diego, CA, USA). Adaptor-ligated DNA fragments 250 bp in length were recovered. The ChIP-seq library was subjected to sequencing with a HiSeq 2000 (Illumina). Total DNA fragments prepared from the shared chromatin fraction (input fraction) were sequenced as the control. ChIP-seq data have been deposited in DDBJ/EMBL/GeneBank under the accession code DRA004110.
Analysis of ChIP-seq data sets
ChIP-seq reads were aligned to the reference mouse genome (mm10) using Bowtie60, version 1.0.0. The multiple-hit reads were excluded, and only the uniquely mapped reads to the reference mouse genome were kept for further analysis. The number of ChIP-seq reads for H3K4me3 mapped around the transcription start site (TSS; 2 kb upstream to 2 kb downstream) was counted using BEDTools61, version 2.17.0. For the analysis of H3K27me3, the number of reads mapped from 2 kb upstream of the TSS to the transcription termination site (TTS) was counted. The number of reads was normalized by the total number of mapped reads to obtain RPM (reads per million). Enrichment values for H3K4me3 and H3K27me3 were defined for each gene as the ratio of RPM in the ChIP fraction to that in the input fraction. Read density profiles of H3K4me3 and H3K27me3 were generated as described previously62.
Measurement of metabolites
Sertoli cells (5 × 105) at P21 were cultured for 3 days. The lactate concentration of 30 μl of the medium was determined at 6 and 24 h after medium change using the Lactate Assay Kit (BioVision, Milpitas, CA, USA). To determine cholesterol synthetic activity, Sertoli cells (1 × 106) were incubated in a serum-free medium containing [1,2–14C]-acetate (PerkinElmer, Inc., Boston, MA USA), 50 μM aminoglutethimide (Sigma) and 2 μg/ml 58–035 (ACAT2; acyl-CoA cholesterol acyltransferase inhibitor; Sigma) for 2.5 or 3 h at 34 °C. All lipids, including cholesterol, were extracted using chloroform/methanol (2:1, v/v), then separated by thin-layer chromatography on silica gel with benzene-ethylacetate (2:3, v/v) as a solvent. The radioactivity of a spot containing free and esterified cholesterol visualized with iodine vapor was determined by liquid scintillation counting. The extent of [1,2-14C]-acetate incorporation into the cholesterol was expressed as cpm/μmol acetate/h.
Measurement of quantities of cholesterol and cholesterol precursors
Sertoli cells (3 × 104) were suspended in 0.2 ml methanol and sonicated (2 × 20-s pulses with 30-s break intervals) using Bioruptor Plus (Diagenode), then centrifuged for 5 min at 15,000 rpm to exclude methanol-insoluble cellular components. The supernatant (methanol-soluble fraction) was recovered and evaporated to remove the methanol. Gas chromatography-mass spectrometry analysis (GC-MS) was performed using an Agilent 6890 Plus gas chromatograph interfaced with a single-quadrupole Agilent 5975 C MSD (Agilent Technologies, Palo Alto, CA, USA) as previously described63.
Determination of testosterone, FSH, and LH concentrations
Blood plasma samples collected individually from 6 XY and 6 XX/Sry mice at P21 were subjected to LC-MS/MS analysis to determine testosterone concentration. The measurement was performed according to a previous report64. Briefly, plasma samples were spiked with 13C3-testosterone and extracted with 1 mL of 90% hexane/10% ethyl acetate (v/v). After evaporation, the samples were reconstituted in 90% methanol/10% H2O (v/v) for LC-MS/ MS analysis. The samples were analyzed on a QTRAP 5500 LC-MS/MS system (AB SCIEX, Framingham, MA) connected to a Shimadzu LC 20 A HPLC system. For determination of FSH and LH concentration, blood plasma samples were collected individually from 7 XY and 6 XX/Sry mice at P21. Plasma FSH and LH concentrations were determined using Rodent FSH ELISA Test Kits and Rodent LH ELISA Test Kits (Endocrine technologies, Inc., Newark, CA), respectively, according to the manufacturer’s instructions.
Statistical analysis
All experiments were performed with at least three biologically independent samples. Data are presented as the mean and standard deviation. The number of the sample is indicated with ‘n’ in figure legends. The statistical significance was examined using a two-tailed Student’s t-test.
Additional Information
How to cite this article: Shishido, Y. et al. Differential lactate and cholesterol synthetic activities in XY and XX Sertoli cells. Sci. Rep. 7, 41912; doi: 10.1038/srep41912 (2017).
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References
Koopman, P., Munsterberg, A., Capel, B., Vivian, N. & Lovell-Badge, R. Expression of a candidate sex-determining gene during mouse testis differentiation. Nature 348, 450–452, doi: 10.1038/348450a0 (1990).
Kashimada, K. & Koopman, P. Sry: the master switch in mammalian sex determination. Development 137, 3921–3930, doi: 10.1242/dev.048983 (2010).
Larney, C., Bailey, T. L. & Koopman, P. Switching on sex: transcriptional regulation of the testis-determining gene Sry. Development 141, 2195–2205, doi: 10.1242/dev.107052 (2014).
Ross, A. J. & Capel, B. Signaling at the crossroads of gonad development. Trends Endocrinol Metab 16, 19–25, doi: 10.1016/j.tem.2004.11.004 (2005).
Koopman, P., Gubbay, J., Vivian, N., Goodfellow, P. & Lovell-Badge, R. Male development of chromosomally female mice transgenic for Sry. Nature 351, 117–121, doi: 10.1038/351117a0 (1991).
Ishii, M. et al. Potency of testicular somatic environment to support spermatogenesis in XX/Sry transgenic male mice. Development 134, 449–454, doi: 10.1242/dev.02751 (2007).
Cattanach, B. M. Sex-reversed mice and sex determination. Ann N Y Acad Sci 513, 27–39 (1987).
Burgoyne, P. S. The role of the mammalian Y chromosome in spermatogenesis. Development 101 Suppl, 133–141 (1987).
Cheng, C. Y., Wong, E. W., Yan, H. H. & Mruk, D. D. Regulation of spermatogenesis in the microenvironment of the seminiferous epithelium: new insights and advances. Mol Cell Endocrinol 315, 49–56, doi: 10.1016/j.mce.2009.08.004 (2010).
Wenger, R. H. & Katschinski, D. M. The hypoxic testis and post-meiotic expression of PAS domain proteins. Seminars in cell & developmental biology 16, 547–553, doi: 10.1016/j.semcdb.2005.03.008 (2005).
Bajpai, M., Gupta, G. & Setty, B. S. Changes in carbohydrate metabolism of testicular germ cells during meiosis in the rat. Eur J Endocrinol 138, 322–327 (1998).
Boussouar, F. & Benahmed, M. Lactate and energy metabolism in male germ cells. Trends Endocrinol Metab 15, 345–350, doi: 10.1016/j.tem.2004.07.003 (2004).
Rato, L. et al. Metabolic regulation is important for spermatogenesis. Nat Rev Urol 9, 330–338, doi: 10.1038/nrurol.2012.77 (2012).
Keber, R., Rozman, D. & Horvat, S. Sterols in spermatogenesis and sperm maturation. J Lipid Res 54, 20–33, doi: 10.1194/jlr.R032326 (2013).
Wiebe, J. P. & Tilbe, K. S. De novo synthesis of steroids (from acetate) by isolated rat Sertoli cells. Biochem Biophys Res Commun 89, 1107–1113 (1979).
Fofana, M., Travert, C., Carreau, S. & Le Goff, D. Evaluation of cholesteryl ester transfer in the seminiferous tubule cells of immature rats in vivo and in vitro . J Reprod Fertil 118, 79–83 (2000).
Gillot, I. et al. Germ cells and fatty acids induce translocation of CD36 scavenger receptor to the plasma membrane of Sertoli cells. J Cell Sci 118, 3027–3035, doi: 10.1242/jcs.02430 (2005).
Selva, D. M. et al. The ATP-binding cassette transporter 1 mediates lipid efflux from Sertoli cells and influences male fertility. J Lipid Res 45, 1040–1050, doi: 10.1194/jlr.M400007-JLR200 (2004).
Vernet, N. et al. Retinoid X receptor beta (RXRB) expression in Sertoli cells controls cholesterol homeostasis and spermiation. Reproduction 136, 619–626, doi: 10.1530/rep-08-0235 (2008).
Volle, D. H. et al. Multiple roles of the nuclear receptors for oxysterols liver X receptor to maintain male fertility. Molecular endocrinology 21, 1014–1027, doi: 10.1210/me.2006-0277 (2007).
Iwase, S. et al. The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases. Cell 128, 1077–1088, doi: 10.1016/j.cell.2007.02.017 (2007).
Hong, S. et al. Identification of JmjC domain-containing UTX and JMJD3 as histone H3 lysine 27 demethylases. Proc Natl Acad Sci U S A 104, 18439–18444, doi: 10.1073/pnas.0707292104 (2007).
Lan, F. et al. A histone H3 lysine 27 demethylase regulates animal posterior development. Nature 449, 689–694, doi: 10.1038/nature06192 (2007).
Santos-Rosa, H. et al. Active genes are tri-methylated at K4 of histone H3. Nature 419, 407–411, doi: 10.1038/nature01080 (2002).
Kirmizis, A. et al. Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27. Genes Dev 18, 1592–1605, doi: 10.1101/gad.1200204 (2004).
Justin, N., De Marco, V., Aasland, R. & Gamblin, S. J. Reading, writing and editing methylated lysines on histone tails: new insights from recent structural studies. Curr Opin Struct Biol 20, 730–738, doi: 10.1016/j.sbi.2010.09.012 (2010).
Lee, S., Lee, J. W. & Lee, S. K. UTX, a histone H3-lysine 27 demethylase, acts as a critical switch to activate the cardiac developmental program. Dev Cell 22, 25–37, doi: 10.1016/j.devcel.2011.11.009 (2012).
Wang, C. et al. UTX regulates mesoderm differentiation of embryonic stem cells independent of H3K27 demethylase activity. Proc Natl Acad Sci USA 109, 15324–15329, doi: 10.1073/pnas.1204166109 (2012).
Welstead, G. G. et al. X-linked H3K27me3 demethylase Utx is required for embryonic development in a sex-specific manner. Proc Natl Acad Sci USA 109, 13004–13009, doi: 10.1073/pnas.1210787109 (2012).
Cahn, R. D., Zwilling, E., Kaplan, N. O. & Levine, L. Nature and Development of Lactic Dehydrogenases: The two major types of this enzyme form molecular hybrids which change in makeup during development. Science 136, 962–969, doi: 10.1126/science.136.3520.962 (1962).
Dawson, D. M., Goodfriend, T. L. & Kaplan, N. O. Lactic Dehydrogenases: Functions of the two Types Rates of Synthesis of the Two Major Forms Can Be Correlated with Metabolic Differentiation. Science 143, 929–933 (1964).
Krieg, A. F., Rosenblum, L. J. & Henry, J. B. Lactate dehydrogenase isoenzymes a comparison of pyruvate-to-lactate and lactate-to-pyruvate assays. Clin Chem 13, 196–203 (1967).
McCullagh, K. J., Poole, R. C., Halestrap, A. P., O’Brien, M. & Bonen, A. Role of the lactate transporter (MCT1) in skeletal muscles. Am J Physiol 271, E143–150 (1996).
Dimmer, K. S., Friedrich, B., Lang, F., Deitmer, J. W. & Broer, S. The low-affinity monocarboxylate transporter MCT4 is adapted to the export of lactate in highly glycolytic cells. Biochem J 350 Pt 1, 219–227 (2000).
Galardo, M. N., Riera, M. F., Pellizzari, E. H., Cigorraga, S. B. & Meroni, S. B. The AMP-activated protein kinase activator, 5-aminoimidazole-4-carboxamide-1-b-D-ribonucleoside, regulates lactate production in rat Sertoli cells. J Mol Endocrinol 39, 279–288, doi: 10.1677/jme-07-0054 (2007).
Goldstein, J. L., DeBose-Boyd, R. A. & Brown, M. S. Protein sensors for membrane sterols. Cell 124, 35–46, doi: 10.1016/j.cell.2005.12.022 (2006).
Pai, J. T., Guryev, O., Brown, M. S. & Goldstein, J. L. Differential stimulation of cholesterol and unsaturated fatty acid biosynthesis in cells expressing individual nuclear sterol regulatory element-binding proteins. J Biol Chem 273, 26138–26148 (1998).
Horton, J. D. et al. Activation of cholesterol synthesis in preference to fatty acid synthesis in liver and adipose tissue of transgenic mice overproducing sterol regulatory element-binding protein-2. J Clin Invest 101, 2331–2339, doi: 10.1172/jci2961 (1998).
Akpovi, C. D., Yoon, S. R., Vitale, M. L. & Pelletier, R. M. The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis. J Lipid Res 47, 2233–2247, doi: 10.1194/jlr.M600162-JLR200 (2006).
Agulnik, A. I. et al. A novel X gene with a widely transcribed Y-linked homologue escapes X-inactivation in mouse and human. Hum Mol Genet 3, 879–884 (1994).
Greenfield, A. et al. The UTX gene escapes X inactivation in mice and humans. Hum Mol Genet 7, 737–742 (1998).
Grootegoed, J. A., Oonk, R. B., Jansen, R. & van der Molen, H. J. Metabolism of radiolabelled energy-yielding substrates by rat Sertoli cells. J Reprod Fertil 77, 109–118 (1986).
Robinson, R. & Fritz, I. B. Metabolism of glucose by Sertoli cells in culture. Biol Reprod 24, 1032–1041 (1981).
Sugathan, A. & Waxman, D. J. Genome-wide analysis of chromatin states reveals distinct mechanisms of sex-dependent gene regulation in male and female mouse liver. Mol Cell Biol 33, 3594–3610, doi: 10.1128/mcb.00280-13 (2013).
Brind’Amour, J. et al. An ultra-low-input native ChIP-seq protocol for genome-wide profiling of rare cell populations. Nat Commun 6, 6033, doi: 10.1038/ncomms7033 (2015).
Smith, L. B. & Walker, W. H. The regulation of spermatogenesis by androgens. Seminars in cell & developmental biology 30, 2–13, doi: 10.1016/j.semcdb.2014.02.012 (2014).
Leader, J. E., Wang, C., Fu, M. & Pestell, R. G. Epigenetic regulation of nuclear steroid receptors. Biochemical pharmacology 72, 1589–1596, doi: 10.1016/j.bcp.2006.05.024 (2006).
Kim, J. Y., Yu, J., Abdulkadir, S. A. & Chakravarti, D. KAT8 Regulates Androgen Signaling in Prostate Cancer Cells. Molecular endocrinology 30, 925–936, doi: 10.1210/me.2016-1024 (2016).
Kidokoro, T. et al. Influence on spatiotemporal patterns of a male-specific Sox9 activation by ectopic Sry expression during early phases of testis differentiation in mice. Dev Biol 278, 511–525, doi: 10.1016/j.ydbio.2004.11.006 (2005).
Nakamura, Y. et al. Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and mediator subunit 25. Nat Commun 2, 251, doi: 10.1038/ncomms1242 (2011).
Shima, Y. et al. Contribution of Leydig and Sertoli cells to testosterone production in mouse fetal testes. Molecular endocrinology 27, 63–73, doi: 10.1210/me.2012-1256 (2013).
Shima, Y. et al. Identification of an enhancer in the Ad4BP/SF-1 gene specific for fetal Leydig cells. Endocrinology 153, 417–425, doi: 10.1210/en.2011-1407 (2012).
Baba, T. et al. Glycolytic genes are targets of the nuclear receptor Ad4BP/SF-1. Nat Commun 5, 3634, doi: 10.1038/ncomms4634 (2014).
Trapnell, C., Pachter, L. & Salzberg, S. L. TopHat: discovering splice junctions with RNA-Seq. Bioinformatics 25, 1105–1111, doi: 10.1093/bioinformatics/btp120 (2009).
Li, B. & Dewey, C. N. RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinformatics 12, 323, doi: 10.1186/1471-2105-12-323 (2011).
Trapnell, C. et al. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol 28, 511–515, doi: 10.1038/nbt.1621 (2010).
Ashburner, M. et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 25, 25–29, doi: 10.1038/75556 (2000).
Huang da, W. et al. Extracting biological meaning from large gene lists with DAVID. Curr Protoc Bioinformatics Chapter 13, Unit 13.11, doi: 10.1002/0471250953.bi1311s27 (2009).
Lorbek, G. et al. Lessons from hepatocyte-specific Cyp51 knockout mice: impaired cholesterol synthesis leads to oval cell-driven liver injury. Scientific reports 5, 8777, doi: 10.1038/srep08777 (2015).
Langmead, B., Trapnell, C., Pop, M. & Salzberg, S. L. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 10, R25, doi: 10.1186/gb-2009-10-3-r25 (2009).
Quinlan, A. R. & Hall, I. M. BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics 26, 841–842, doi: 10.1093/bioinformatics/btq033 (2010).
Gafni, O. et al. Derivation of novel human ground state naive pluripotent stem cells. Nature 504, 282–286, doi: 10.1038/nature12745 (2013).
Son, H. H. et al. High-temperature GC-MS-based serum cholesterol signatures may reveal sex differences in vasospastic angina. J Lipid Res 55, 155–162, doi: 10.1194/jlr.D040790 (2014).
Star-Weinstock, M., Williamson, B. L., Dey, S., Pillai, S. & Purkayastha, S. LC-ESI-MS/MS analysis of testosterone at sub-picogram levels using a novel derivatization reagent. Analytical chemistry 84, 9310–9317, doi: 10.1021/ac302036r (2012).
Acknowledgements
This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 21249018 and 16H05142 (K. Mo.), Ministry of Education, Culture, Sports, Science, and Technology, Japan (MEXT) KAKENHI Grant Number 22132002 (K. Mo.), the Uehara Memorial Foundation, and Takeda Science Foundation (T.B.).
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Y. Shishido, T.B., M.I. and K. Mo. conceived and designed the experimental approach and performed the experiments. T.S. and M.S. contributed to the computational analyses for mRNA-seq and ChIP-seq. H.A. and Y.K. provided the mice. H.K. produced anti-H3K4me3 and anti-H3K27me3 antibodies, and R.D produced anti-CYP51 antibody. Y.I. and M-H.C. measured cholesterol metabolites. S.H. and A.M. determined testosterone concentration. Y.O. performed deep sequencing of the mRNA-seq and ChIP-seq libraries. Y. Shima and K. Mi. provided technical support and conceptual advice. Y. Shishido, T.B., and K. Mo. analyzed the data and prepared the manuscript.
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Shishido, Y., Baba, T., Sato, T. et al. Differential lactate and cholesterol synthetic activities in XY and XX Sertoli cells. Sci Rep 7, 41912 (2017). https://doi.org/10.1038/srep41912
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DOI: https://doi.org/10.1038/srep41912
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