Abstract
Bacteria are thought to avoid using the essential metal ion copper in their cytosol due to its toxicity. Herein we characterize Csp3, the cytosolic member of a new family of bacterial copper storage proteins from Methylosinus trichosporium OB3b and Bacillus subtilis. These tetrameric proteins possess a large number of Cys residues that point into the cores of their four-helix bundle monomers. The Csp3 tetramers can bind a maximum of approximately 80 Cu(I) ions, mainly via thiolate groups, with average affinities in the (1–2) × 1017 M−1 range. Cu(I) removal from these Csp3s by higher affinity potential physiological partners and small-molecule ligands is very slow, which is unexpected for a metal-storage protein. In vivo data demonstrate that Csp3s prevent toxicity caused by the presence of excess copper. Furthermore, bacteria expressing Csp3 accumulate copper and are able to safely maintain large quantities of this metal ion in their cytosol. This suggests a requirement for storing copper in this compartment of Csp3-producing bacteria.
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Introduction
Cells do not typically store transition metal ions. The most notable exceptions are iron that is stored by ferritins1 and zinc and copper sequestration by metallothioneins (MTs)2. Copper is essential for almost all organisms yet can also be harmful due to its redox activity and the ability to bind to sites for other metals, particularly iron-sulfur clusters3. This has resulted in the evolution of intricate homeostatic systems that facilitate copper’s use as the cofactor for many important enzymes in both eukaryotes and prokaryotes4,5,6. The response to excess copper regularly involves a copper-exporting P-type ATPase4,5,6,7. Also present are the cytosolic copper metallochaperone ATOX1 (HAH1) in humans, Atx1 in yeast and CopZ in many bacteria, which bind Cu(I) tightly4,5,6,8,9,10,11, and are thought to deliver the metal to ATPases4,5,6. In addition to efflux systems, eukaryotes possess the cytosolic Cys-rich MTs2,4,5, which can fold around multiple Cu(I) ions forming thiolate-coordinated clusters2,12,13. Related copper-binding bacterial MTs have only been found to date in certain pathogenic mycobacteria14, and most bacteria are currently not thought to maintain copper in the cytosol5,15. The interplay between copper homeostasis in bacteria and humans is of medical importance as copper appears to be exploited as a weapon by the mammalian immune system to fight bacterial pathogens, and copper homeostasis genes provide a fitness advantage during infection16.
Methane oxidizing bacteria (methanotrophs) require large amounts of copper for the active site of the membrane-bound (particulate) methane monooxygenase (pMMO)17. Some methanotrophs, including Methylosinus trichosporium OB3b, can use a soluble iron sMMO when copper levels are low, with switchover regulated by copper18. MMOs and methanotrophs have great potential for biotransformations giving a range of products, in bioremediation and for mitigating the release of methane, a potent greenhouse gas, to the atmosphere19,20,21. Understanding copper handling and the expression of either pMMO or sMMO is essential for all these biotechnological applications. Recently, a new family of bacterial copper storage proteins, the Csps, has been discovered in M. trichosporium OB3b22. Three Csps are present in this model methanotroph; two (MtCsp1 and MtCsp2) with predicted twin arginine translocase (Tat) targeting signal peptides are exported from the cytosol. MtCsp1 has been characterized and is a tetramer that can bind up to 52 Cu(I) ions mainly via Cys residues22. Deletion of the genes for MtCsp1 and MtCsp2 increases the rate of switchover from pMMO to sMMO in M. trichosporium OB3b, implicating these proteins in the storage of copper for methane oxidation22. M. trichosporium OB3b also possesses a Csp3 that lacks a signal peptide and must be cytosolic, with homologues of this protein present in a wide range of bacteria (Supplementary Fig. S1).
pMMO is housed on specialized intracytoplasmic membranes23 that are either contiguous with the periplasm or form discrete compartments and thus pMMO may be a rare example of a bacterial cytoplasmic copper enzyme. M. trichosporium OB3b possesses homologues of the copper-efflux ATPase CopA7 and the copper-dependent transcriptional activator CueR24, but lacks CopZ25. However, this methanotroph produces methanobactin (mbtin), a small modified peptide that is secreted and can sequester copper18,26,27,28,29. Copper-loaded mbtin is imported by MbnT, a TonB-dependent transporter30, but other copper uptake mechanisms are also present30,31. It has been suggested that CopD is an inner membrane copper importer in M. trichosporium OB3b, and may play a role in non-mbtin-bound copper uptake32. Although both mbtin and CopD are implicated in delivering copper to the cytosol in M. trichosporium OB3b, how this is safely handled is currently unknown.
A cytosolic Csp3 is also present in Bacillus subtilis, whose metal ion homeostasis has been studied in detail33,34,35. This model Gram positive bacterium possesses the copper-dependent transcriptional activator CsoR36,37,38, which regulates expression of both CopA and CopZ (BsCopZ)36. The deletion of copZ, and particularly copA, increases sensitivity to elevated copper levels39,40, with the ΔcopA strain exhibiting a 3-fold decrease in cellular copper content, whilst the absence of CopZ causes copper levels to increase 2.6 fold40. B. subtilis is known to possess two classes of copper-requiring enzymes; cytochrome oxidases41,42 and a multi-copper oxidase (CotA)43, located on the cytoplasmic membrane and spore surface respectively. A bioinformatics study also suggested the presence of the Cu, Zn superoxide dismutase SodC44. However, in vitro analysis of this protein, which lacks two of the copper ligands, demonstrates that it cannot bind copper and has no superoxide dismutase activity45. Regardless of the lack of a currently identified intracellular requirement for copper, a protein (YcnJ) with homology to both CopD and CopC, the latter a periplasmic copper metallochaperone found in Gram negative bacteria, at its C- and N-termini respectively has been suggested to be involved in copper uptake into the cytosol of B. subtilis46.
To understand the role of Csp3s in cytosolic copper-handling by Gram negative and Gram positive bacteria we have characterized the proteins from M. trichosporium OB3b (MtCsp3) and B. subtilis (BsCsp3). This has included investigating their Cu(I)-binding and release properties in vitro. Furthermore, the ability of BsCsp3 to buffer copper and prevent toxicity in vivo has been analysed. Overall, these studies highlight significant functional diversity of the cytosolic Csp3s relative to their exported counterparts, and demonstrate that as in eukaryotes, Csp3-containing bacteria possess a storage mechanism in addition to efflux, as a response to copper toxicity. Csp3 provides a safe cytosolic source of copper, a finding of fundamental importance for understanding how bacteria utilize and respond to this metal.
Results
Protein quantification
Precise protein quantification is essential for the in vitro studies described herein with the Csp3s. The large number of Cys residues (18 in MtCsp3 and 19 in BsCsp3) allows the use of the 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB, Ellman’s reagent) assay for precise quantification, but only for the unfolded proteins as there is little reactivity in the absence of denaturant (see Supplementary Methods). Unfolding is readily achieved for apo-MtCsp3 using urea (Supplementary Fig. S2) and for apo-BsCsp3 with guanidine hydrochloride (Supplementary Fig. S3), but not for either Cu(I)-protein (Supplementary Figs S2 and S3). Therefore, for Cu(I)-Csp3 samples the number of Cu(I) equivalents quoted is typically based on apo-protein concentrations determined by DTNB. Quantification of the Csp3s with the Bradford assay gives erroneous protein concentrations. Nevertheless, the values obtained with this assay are reproducible and the resulting Bradford:DTNB concentration ratio provides a method of detecting disulfide-bond formation in such Cys-rich proteins. A Bradford:DTNB ratio of 1.44 ± 0.13 (n = 77) was obtained for apo-MtCsp3 not treated with dithiothreitol (DTT), with a ratio of 1.40 ± 0.12 (n = 39) for protein that was incubated overnight with DTT. For apo-BsCsp3 the corresponding samples gave Bradford:DTNB ratios of 1.31 ± 0.11 (n = 49) and 1.25 ± 0.12 (n = 12) respectively. Treatment with DTT has no significant effect on thiol quantification demonstrating that the Cys residues of both apo-Csp3s are not readily susceptible to oxidation. This is confirmed by prolonged incubation in air after treatment with DTT (see Supplementary Methods) having almost no effect on the Bradford:DTNB ratio.
The crystal structures of apo-Csp3s
In the crystal structure, apo-MtCsp3 is a four-helix bundle consisting of ~73% α-helical secondary structure (Figs 1a and 2a), very similar to that calculated (76.0 ± 2.8%, n = 10) from far-UV circular dichroism (CD) spectra (Supplementary Fig. S5). Apo-MtCsp3 is a tetramer in the crystal (Fig. 1a), and in solution at higher protein concentrations (above ~80 μM) elutes from a gel-filtration column largely as a single peak with an apparent molecular weight (n = 31) of 45.2 ± 4.1 kDa (Supplementary Fig. S5). The discrepancy between this and the actual molecular weight of the MtCsp3 tetramer (58.1 kDa) is due to the N-terminal α-helix (αN), which does not significantly increase the volume of the tetramer (Fig. 1a). The 18 Cys residues, all originating from α-helices, point into the core of the four-helix bundle of an apo-MtCsp3 monomer and none are involved in disulfide bonds (Figs 1a and 2a), consistent with the Bradford:DTNB ratio data above. The crystal structure of apo-BsCsp3 has also been determined (Figs 1b and 2b) and the protein is a four-helix bundle like that of apo-MtCsp3 (the α-helical content in the structure is ~81%, very similar to the value of 83.1 ± 2.5% (n = 3) obtained from far-UV CD measurements, Supplementary Fig. S5). Apo-BsCsp3 also forms a tetramer, but αN is missing (Fig. 1b and Supplementary Fig. S4) and consequently the apparent molecular weight in solution (46.0 ± 1.8 kDa, n = 8, Supplementary Fig. S5) matches that expected (47.4 kDa). The 19 Cys residues, also all from α-helices, point into the core of the bundle of apo-BsCsp3, with evidence of partial disulfide bond formation only between Cys38 and Cys81 in the crystal (Fig. 2b and Supplementary Fig. S4). In both Csp3 structures three highly conserved His residues (Supplementary Fig. S1) are found at the more solvent exposed end of the four helix bundle (Figs 1 and 2a,b).
Cu(I)-binding stoichiometry of Csp3s
Cu(I) titrations, monitored using the formation of (S)Cys → Cu(I) ligand-to-metal charge transfer bands below 350 nm12,22,47, give approximate Cu(I)-binding stoichiometries for monomers of 15 to 20 (n = 13) and 17 to 21 (n = 8) for MtCsp3 (Supplementary Fig. S6) and BsCsp3 (Supplementary Fig. S7) respectively. More precise Cu(I)-binding stoichiometries are obtained in the presence of a relatively small excess (~40-fold) of the high-affinity chromophoric Cu(I) ligand bicinchoninic acid (BCA, logβ2 = 17.7 (ref. 48)), showing that under these conditions MtCsp3 (n = 8) and BsCsp3 (n = 10) bind all Cu(I) until 17.9 ± 1.0 (Fig. 3a) and 19.6 ± 0.8 (Fig. 3b) equivalents respectively have been added per monomer. Cu(I)-thiolate clusters, such as those found in MTs, typically give rise to luminescence in the 500 to 700 nm region12,14 due to solvent protected Cu(I)-Cu(I) interactions12,49,50,51. Such luminescence is observed and increases until ~9 to 11 (Supplementary Fig. S6) and ~8 to 12 (Supplementary Fig. S7) equivalents of Cu(I) per monomer are added to MtCsp3 (n = 8) and BsCsp3 (n = 3) respectively. Emission then decreases to almost zero upon addition of further Cu(I), either due to structural modification of the original sites or an increase in their solvent exposure49,50,51.
Crystal structure of Cu(I)-MtCsp3
The structure of MtCsp3 is hardly affected (rmsd 0.32 Å for 116 aligned Cα atoms) by fully loading the protein with Cu(I) (Fig. 4a and Supplementary Fig. S8), consistent with far-UV CD data (α-helical content of 74.8 ± 2.2%, n = 4, Supplementary Fig. S5). The same tetrameric arrangement is present for Cu(I)-MtCsp3 as for the apo-protein, in agreement with gel-filtration studies that give an apparent molecular weight (n = 12) of 44.6 ± 1.8 kDa (Supplementary Fig. S5). The anomalous difference density for data collected just below the copper-edge identifies 19 copper ions within the core of the four-helix bundle of Cu(I)-MtCsp3 bound predominantly by Cys residues (Fig. 4a), consistent with Cu(I)-binding data (Fig. 3a and Supplementary Fig. S6). BsCsp3 has a similar, if not slightly greater, Cu(I) capacity (see above), probably due to the presence of one more Cys residue. Like MtCsp3, Cu(I) binding to BsCsp3 does not affect either its secondary (76.0 ± 2.6% α-helical content, n = 3, Supplementary Fig. S5) or quaternary (43.3 ± 0.8 kDa, n = 8, Supplementary Fig. S5) structure.
The Cu(I) ions are located along the core of the four-helix bundle of MtCsp3 (Fig. 4a). The majority of the sites involve Cu(I) coordinated by the thiolates of two Cys residues in an arrangement (Fig. 4b,c) reminiscent of that found in Cu(I) metallochaperones15,47,52, Cu(I) sensors24,37, and also MtCsp122. In Cu(I)-MT most sites are coordinated by three Cys ligands13, but only Cu15 (Fig. 4c) is ligated by three thiolates in Cu(I)-MtCsp3. There are sites with unusual Cu(I) coordination such as Cu13 (Fig. 4c), which not only involves ligation by Cys101 and Cys114 (Cu(I)–S(Cys) bond lengths of 2.2 Å and a S(Cys)–Cu–S(Cys) angle of 148°) but also the Oδ1 of Asn58 at 2.4 Å (S(Cys)–Cu–O(Asn) angles of 103° to 109°). Furthermore, Cu18 and Cu19, located at the end of the four-helix bundle at which Cu(I) sites are more solvent exposed, both involve coordination by one of the His residues found in this region (Fig. 4c). Cu18 is bound by His110 (Nδ1) and Cys111 with Cu(I)–ligand distances of 2.1 Å and a S(Cys)–Cu–N(His) angle of 165°, whilst there are two Cys ligands (Cys54 and Cys111) as well as the coordinating His104 (Nδ1) at Cu19 (Cu(I)–ligand distances of 2.2 to 2.4 Å and bond angles of 105° to 135°). Numerous Cu(I)-Cu(I) interactions are present in the core of Cu(I)-MtCsp3, with 18 of the sites being 2.5 to 2.8 Å from an adjacent Cu(I) ion, and Cu5, Cu7, Cu9 and Cu13 are involved in three Cu(I)-Cu(I) interactions of <2.8 Å.
Average Cu(I) affinities of the Csp3s
To estimate average Cu(I) affinities of the Csp3s the chromophoric ligand bathocuproine disulfonate (BCS, logβ2 = 20.8)48 was mainly used to buffer free Cu(I). An association constant (n = 5) of (1.7 ± 0.5) × 1017 M−1 was obtained for MtCsp3 with a Hill coefficient of 1.0 ± 0.1 (Fig. 3c,d). The calculated occupancies of 16.2 to 16.9 per monomer for the MtCsp3 samples from Fig. 3c incubated with 36.6 to 39.0 equivalents of Cu(I) for 67 h (see Supplementary Fig. S9 for the stability of MtCsp3 over time) were checked. To do this the protein was separated from [Cu(BCS)2]3− and free BCS, and an MtCsp3 monomer was found to bind 20.9 equivalents of Cu(I). Using BCA as the Cu(I)-buffering ligand (n = 1), a Cu(I) affinity (association constant) of (2.0 ± 0.1) × 1017 M−1 (Hill coefficient of 1.3 ± 0.1) was obtained (86 h incubation), but the maximum calculated occupancy was low (~11), although an MtCsp3 monomer bound 16.0 equivalents of Cu(I) when separated from [Cu(BCA)2]3− and free BCA (see Supplementary Methods). For BsCsp3 (n = 3) the average Cu(I) affinity (association constant) is (1.5 ± 0.4) × 1017 M−1, with a Hill coefficient of 0.9 ± 0.1 (Fig. 3e,f). The calculated occupancies of 17.8 to 18.6 equivalents for samples from Fig. 3e to which 36.6 to 39.5 equivalents of Cu(I) had been added (after 138 h incubation, see Supplementary Fig. S9 for the stability of BsCsp3 over time), were found to bind 20.2 equivalents of Cu(I) per monomer after separation from [Cu(BCS)2]3− and free BCS.
Cu(I) removal from Csp3s
Mbtin from M. trichosporium OB3b has a very high Cu(I) affinity27,48 of (6–7) × 1021 M−1 at pH 7.5 and removes all Cu(I) from MtCsp3 loaded with 18.0 Cu(I) equivalents (Fig. 5a), but this takes approximately 15 days. Cu(I) removal from Cu(I)-BsCsp3 by apo-BsCopZ, which has a Cu(I) affinity8,9,48 of ~1018 M−1, has also been analyzed. After 64 h (Fig. 5b) ~35–45% of Cu(I) transfers from BsCsp3 to BsCopZ (see Supplementary Methods). Thus Cu(I) removal by possible physiological partners is very slow for both Csp3s. The ability of Csp3s to hold onto Cu(I) was further investigated using BCS, with a very large excess removing only ~20% of Cu(I) from MtCsp3 after 85 h (Fig. 5c), whilst ~85% removal from BsCsp3 is observed over the same timescale (Fig. 5d).
In vivo studies of BsCsp3
To test the ability of Csp3 to provide protection against copper toxicity, BsCsp3 was introduced into a ΔcopA E. coli strain, which exhibited diminished growth upon increasing copper concentration in the media compared to wild type (WT) (Fig. 6a)53. The expression of BsCsp3 reverses this effect allowing ΔcopA E. coli to grow for significantly longer than WT, particularly at 0.5 to 1.5 mM copper (Fig. 6b–f). Furthermore, cells grown under these conditions expressing BsCsp3 accumulate approximately two times more copper than ΔcopA control cells. An initial analysis of the Δcsp3 strain of B. subtilis has also been undertaken (see Supplementary Methods), which exhibits enhanced cell death compared to WT in the presence of 1.5 to 2.0 mM copper (Supplementary Fig. S11).
Discussion
The Csp3s are the cytosolic members of a new family of copper storage proteins22. Both the M. trichosporium OB3b and B. subtilis proteins form tetramers of four-helix bundles (Fig. 1) possessing many Cys residues pointing into their cores, and can accommodate up to approximately 80 Cu(I) ions per tetramer. Csp3s are present in many other bacteria (Supplementary Fig. S1) and presumably have similar structures and Cu(I)-binding capabilities to the proteins studied here. The Cu(I) capacity of the Csp3s is much greater than that of a MtCsp1 tetramer, which binds a maximum of 52 Cu(I) ions22. The number of Cu(I) ions a Csp can bind is dictated by how many Cys residues it possesses, with 18 and 19 respectively per monomer in MtCsp3 and BsCsp3 compared to 13 in MtCsp1. Copper coordination in MtCsp3 largely involves sites with two Cys ligands, with the most novel being Cu13, which as well as having two coordinating thiolates is bound by the side chain amide oxygen of Asn58 (Fig. 4c). This Asn is not highly conserved in Csp3s (Supplementary Fig. S1), and an Asp is more common, as found in BsCsp3 (Fig. 2b). The high density of Cys residues within the core of the Csp3s and the absence of facile disulfide bond formation is remarkable. The Cys residues all originate from α-helices and the four-helix bundle scaffold provides the rigidity required to hold these residues sufficiently far apart to prevent thiol groups coupling via oxidation (in a cell this will be assisted by the reducing environment of the cytosol). This is essential as disulfide formation would hinder, and perhaps even prevent, Cu(I) binding.
The lack of cooperativity of Cu(I) binding by the Csp3s highlights that their Cu(I)-uptake mechanism probably differs compared to that of MtCsp1 (the Hill coefficient is close to 1 for both Csp3s, whereas for MtCsp1 it is ~3, indicating positive cooperativity). This is consistent with luminescence at around 600 nm for Csp3s upon Cu(I) addition (Supplementary Figs S6 and S7), whereas very little emission is observed for MtCsp122. The average Cu(I) affinities of MtCsp3, BsCsp3 and MtCsp1 are all in the (1–2) × 1017 M−1 range. The slow removal of Cu(I) by a range of partners, all with tighter Cu(I) affinities, is a highly unusual feature of the Csp3s compared to the exported MtCsp122 and MT54 (the eukaryotic cytosolic Cu(I) storage protein), which can both be very quickly stripped of all Cu(I). Removal of iron from the mineral core of ferritin can also occur on a similarly short timescale1,55. A comparison of Cu(I)-removal rates for MtCsp3, BsCsp3 and MtCsp1 is obtained using BCS. Cu(I) removal by this high affinity small molecule ligand is slowest for MtCsp3, faster for BsCsp3 (Fig. 5c,d), but is orders of magnitude quicker for MtCsp1, with all Cu(I) removed in ~30 min22.
One end of a Csp four-helix bundle is hydrophobic whilst at the opposite end the Cu(I) sites are more solvent exposed (Fig. 2), which presumably is the route via which Cu(I) ions enter and leave. Structural variations between the Csp3s and MtCsp1 at this opening could be responsible for the dramatically different rates of Cu(I)-removal. His104, His108 and His110 (His104 and His110 are ligands, Fig. 4c) from the α3-loop-α4 region are found around the opening to the bundle in MtCsp3 (Figs 1a and 2a) and are highly conserved among Csp3s (Supplementary Fig. S1), corresponding to His84, His86 and His88 in BsCsp3 (Figs 1b and 2b). At the entrance of the MtCsp1 bundle (Fig. 2c)22 there is only a single His residue (His36 that coordinates Cu13) and three largely conserved methionines; Met40, Met43 and Met48 (the latter is a bridging ligand between Cu11 and Cu13), on α1 and the loop linking α1 with α2. Leu51 of MtCsp3 (Fig. 2a), which is conserved in BsCsp3 (Leu35, Fig. 2b), corresponds to Met48 in MtCsp1 (Fig. 2c) and its hydrophobic side chain appears to partially block the entrance (a Met is actually found in this position in a number of Csp3s, Supplementary Fig. S1). The residues at the opening of the bundle could influence Cu(I)-removal rates from the cores of these proteins.
Physiologically, MtCsp3 could acquire copper imported either via the mbtin-dependent uptake system or from CopD. BsCsp3 may receive copper via YcnJ, which has homology to CopD. It is possible that Cu(I) removal from MtCsp3 is carried out by apo-mbtin when copper availability decreases in M. trichosporium OB3b, although in vitro this process occurs very slowly. B. subtilis is not known to possess a high affinity Cu(I) ligand, but it does produce bacillithiol56. However, this small molecule antioxidant has been implicated in Zn(II) buffering57. Cu(I) acquisition from BsCsp3 by apo-BsCopZ, the cytosolic copper metallochaperone not present in M. trichosporium OB3b, is also slow in vitro. In B. subtilis and M. trichosporium OB3b Cu(I) could be passed from Csp3 to a metal-binding domain of CopA that has a very similar structure to CopZ47,52, or to the copper-dependent transcriptional activators, CueR and CsoR respectively. However, these all bind Cu(I) in a similar manner to CopZ15, and therefore transfer is also expected to be slow. Once bound by Csp3, Cu(I) ions appear to be trapped, which would be expected to help ensure non-specific removal in the cytosol preventing toxicity. This is consistent with the ability of BsCsp3 to allow the ΔcopA E. coli strain to grow for significantly longer than WT at copper concentrations above 0.5 mM (Fig. 6c–e) and to accumulate intracellular copper. Furthermore, Δcsp3 B. subtilis exhibits enhanced cell death above 1.0 mM copper compared to WT (Supplementary Fig. S11). The Csp3s can bind copper in the cytosol of bacteria conferring the ability to grow at elevated levels of this metal.
Slow removal of Cu(I) from Csp3s observed in vitro implies that either these proteins are involved in long-term copper storage or that mechanisms are present in cells to facilitate the delivery of their metal cargo to specific targets. MtCsp3 could act as a longer-term store of copper for pMMO in M. trichosporium OB3b, compared to more immediate supply22 by MtCsp1 and MtCsp2. Transcriptomic studies have shown that the csp3 gene is up-regulated in B. subtilis under various stress-inducing/spore-forming conditions58. One of many outer spore coat proteins is CotA, a laccase belonging to the multi-copper oxidase family43, and BsCsp3 may store copper for this enzyme. Furthermore, in both organisms Csp3-bound copper potentially supplies currently unknown copper enzymes. This is consistent with the number of high-abundance copper fractions that are resolved from soluble extracts of M. trichosporium OB3b22. Some Csp3-containing bacteria are pathogenic and Csp3 could be important in allowing these to respond to copper-related attack by a host’s immune system16. In conclusion, the presence of Csp3 will allow a bacterium to maintain a safe, and potentially large, cytosolic source of copper, and work is currently underway to identify physiological targets for Csp3-bound Cu(I).
Materials and Methods
Protein production and analysis
The methods used to clone, over-express, purify, quantify and analyze the Csp3s and BsCopZ are similar to those described previously22,59, and are given in detail in the Supplementary Methods. Protein concentrations were determined using the DTNB assay, with unfolded apo-proteins when quantifying Csp3s, under anaerobic conditions22,47. Csp3 concentrations were also determined with the Bradford assay. Proteins were analyzed using atomic absorption spectrometry (AAS), far-UV (180–250 nm) CD spectroscopy and analytical gel-filtration chromatography9,10,22,47,60,61.
Preparation of Cu(I) samples, Cu(I)-binding stoichiometry, estimated average Cu(I) affinities and Cu(I) removal
Most experiments were performed in 20 mM Hepes plus 200 mM NaCl at pH 7.5 and are described in detail in the Supplementary Methods. Work with Cu(I) and Cu(I)-proteins was performed under anaerobic conditions22,47. To prepare Cu(I)-Csp3 samples, the appropriate amount of a buffered Cu(I) solution (made from a Cu(I) stock in acetonitrile) was mixed with apo-protein quantified by DTNB in the presence of either urea or guanidine hydrochloride, with Cu(I) equivalents per monomer quoted in all cases. Direct Cu(I) titrations into apo-Csp3s were monitored by UV-Vis and fluorescence spectrophotometry. To determine the number of tightly bound equivalents per monomer, Cu(I) was added to apo-Csp3 in the presence of ~40 equivalents of BCA and the formation of [Cu(BCA)2]3− quantified by UV-Vis spectrophotometry22,60. Average Cu(I) affinities were measured mainly using BCS and an approach described previously22,54. For these measurements mixtures of Csp3 (~2.5 μM, 2.09 μM on one occasion for BsCsp3) and BCS (100–121 μM) plus increasing concentrations of Cu(I) were incubated anaerobically for up to 124 h for MtCsp3 and 138 h for BsCsp3, with reported affinities from data measured after 42–43 h and 90–96 h respectively (no significant change observed at the longer incubation times). The ability of M. trichosporium OB3b mbtin to remove Cu(I) from MtCsp3, and Cu(I) removal from MtCsp3 and BsCsp3 by BCS, were both monitored by UV-Vis spectrophotometry22. Cu(I) removal from Cu(I)-BsCsp3 by apo-BsCopZ was followed by analyzing an anaerobic mixture of the proteins using gel-filtration chromatography. Many of the above experiments required Csp3 samples to be incubated over many days and the stability of proteins over the same time period was tested by far-UV CD spectroscopy.
In vivo studies using the copA deletion strain of Escherichia coli
The published53 copper-dependent phenotype for a ΔcopA E. coli was confirmed as described in the Supplementary Methods. BsCsp3 was sub-cloned from pET29a into the XbaI and HindIII sites of pBAD33 giving pBAD33_BsCsp3. The growth of ΔcopA transformed with pBAD33_BsCsp3 was investigated at a range of copper concentrations (details in the Supplementary Methods). Copper levels in cells were measured by AAS as described in the Supplementary Methods.
Crystallization, data collection, structure solution and refinement
Diffraction-quality crystals were obtained as described in the Supplementary Methods. All crystallographic data were collected at Diamond Light Source Ltd, UK (beamline I02) and structures were solved by molecular replacement using Molrep implemented via the CCP4 suite62. Models underwent building cycles in Coot63 and refinement in REFMAC564. Five percent of observations were used to monitor refinement. All models were validated using Molprobity65 and data collection statistics and refinement details are reported in Supplementary Table S1. Atomic coordinates have been deposited in the Protein Data Bank under accession numbers 5ARM, 5ARN and 5FIG for apo-MtCsp3, Cu(I)-MtCsp3 and apo-BsCsp3 respectively.
Additional Information
How to cite this article: Vita, N. et al. Bacterial cytosolic proteins with a high capacity for Cu(I) that protect against copper toxicity. Sci. Rep. 6, 39065; doi: 10.1038/srep39065 (2016).
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Acknowledgements
We thank staff of the DLS synchrotron radiation source for help with diffraction data collection. This work was supported by Biotechnology and Biological Sciences Research Council (grant BB/K008439/1 to C.D. and K.J.W.), by Newcastle University who part-funded a PhD studentship (S.P.) and by a John William Luccock and Ernest Jeffcock studentship (G.L.). K.J.W. is supported by a Sir Henry Dale Fellowship funded by the Wellcome Trust and the Royal Society (098375/Z/12/Z).
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C.D. designed the research with help from K.J.W.; N.V., G.L. and S.P. performed in vitro characterization of the Csp3s and Cu(I) binding, S.P. and G.L. crystallized MtCsp3 and BsCsp3 respectively, A.B. solved the crystal structures and N.V. and J.L. performed in vivo studies. C.D. wrote the paper and all authors commented on the manuscript.
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Vita, N., Landolfi, G., Baslé, A. et al. Bacterial cytosolic proteins with a high capacity for Cu(I) that protect against copper toxicity. Sci Rep 6, 39065 (2016). https://doi.org/10.1038/srep39065
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DOI: https://doi.org/10.1038/srep39065
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