Intestinal epithelial cells (IECs) play an indispensable role in maintaining body fluid balance partly through their ability to regulate anion/fluid secretion. Yet in various inflammatory gastrointestinal diseases, over-secretion of anions results in symptoms such as severe diarrhoea. Endogenous mediators, such as vasoactive intestinal peptide or prostaglandin E2 (PGE2), regulate intestinal anion/fluid secretion, but their direct effect on purified human IECs has never been described in detail. Based on a previously described intestinal organoid swelling model, we established a 3D-scanner-assisted quantification method to evaluate the anion/fluid secretory response of cultured human IECs. Among various endogenous secretagogues, we found that PGE2 had the lowest EC50 value with regard to the induction of swelling of the jejunal and colonic organoids. This PGE2-mediated swelling response was dependent on environmental Cl− concentrations as well as on several channels and transporters as shown by a series of chemical inhibitor studies. The concomitant presence of various inflammatory cytokines with PGE2 failed to modulate the PGE2-mediated organoid swelling response. Therefore, the present study features PGE2 as a direct and robust mediator of anion/fluid secretion by IECs in the human intestine.
The intestinal mucosa plays an indispensable role in the homeostasis of anion and body fluid maintenance. The balance between fluid absorption and fluid secretion is finely regulated by various endogenous factors to maintain a proper inner-body fluid volume1. Studies have suggested that a gradient of these factors exists along the cephalocaudal axis and along the crypt-villus axis2,3.
Intestinal epithelial cells (IECs) directly regulate the absorption and release of fluids through the transport of anions in a basal-to-apical movement as well as an apical-to-basal movement. These functions of IECs are executed at the molecular level by the coordinated ion transport via membrane-bound transporters and channels at both the apical and basolateral membranes.
Fluid secretion by IECs is mainly regulated through the secretion of the Cl− ion. The major transporter for Cl− secretion on the apical surface of IECs is the cystic fibrosis transmembrane conductance regulator (CFTR)4. CFTR is activated through an increase of intracellular cAMP and thereby functions as a Cl− channel as well as an HCO3− channel5,6. Ca2+-dependent Cl− channels (CaCCs) are another group of channels on the apical surface that can secrete Cl− 7. On the basolateral membrane, Na-K-2Cl cotransporter 1 (NKCC1) contributes to the uptake of Cl−, and the Na+/K+ ATPase and K+ channels, such as potassium voltage-gated channel subfamily Q member 1 (KCNQ1) and potassium calcium-activated channel subfamily N member 4 (KCNN4), support the function of NKCC1 by producing an electrochemical gradient across the luminal membrane8. Forced activation of this Cl− secretion system by external stimuli, such as by the cholera toxin, leads to the excess secretion of fluids and manifests as a secretory-type diarrhoea9.
Various endogenous secretagogues are implicated in the regulation of Cl− and fluid secretion by IECs. Neurotransmitters, such as acetylcholine (ACh) and vasoactive intestinal peptide (VIP), induce Cl− secretion through muscarinic receptors and VIP receptors, respectively10,11. Serotonin and histamine are secreted by mast cells and promote Cl− secretion by IECs through either a direct or indirect effect12,13. Additionally, the production of bradykinin is upregulated in the inflammatory environment and thereby promotes Cl− secretion through B2 receptors expressed in IECs14.
Prostaglandin E2 (PGE2) is another endogenous secretagogue that may directly promote Cl− secretion from IECs15. PGE2 is generated through the prostaglandin cascade and is mainly produced by mesenchymal cells in the intestinal mucosa16. The function of PGE2 is highly pleiotropic, as it can regulate the secretion of anions17 and mucus18, GI motility19, and the survival and proliferation of IECs20,21. PGE2 may also contribute to the maintenance of the stem-like properties of IECs through the enhancement of Wnt signalling22,23. In addition, PGE2 is one of the inflammatory mediators upregulated in inflammatory bowel disease (IBD)24,25. In the inflammatory environment, PGE2 may directly exert its effect on IECs and contribute to symptoms such as diarrhoea in IBD patients26. However, it is unclear to what extent PGE2 affects Cl− secretion by human IECs. As most of the previously related studies were based on either carcinoma-derived cell lines, such as T84 or HT29, or on biopsies, there is a lack of data based on truly benign purified human IECs.
A recent advance in culture techniques has made it possible to maintain benign IECs in vitro for a desired period27,28,29. Crypt cells can be maintained in a 3D-structure referred to as organoids, and those organoids have been shown to serve as a suitable model to evaluate various IEC-specific functions30,31. A recent study suggested that the addition of forskolin (FSK) can induce forced fluid influx towards the inner space of the organoids and result in forskolin-induced swelling (FIS)32. Such a response is mediated by the function of CFTR and thus has been suggested as a useful index to measure its function in cystic fibrosis patients33. This study also showed that the FIS response might be extended to analyse the anion/fluid secretion of IECs that are triggered by other external stimuli.
In this study, we used the FIS technique and modified it to efficiently quantify the response of a large number of samples. Using our analysis system, we tested the response of human intestinal organoids based on cell samples from IBD patients to various endogenous secretagogues, including PGE2. Among the tested candidates, PGE2 showed the lowest EC50 value both in the small intestinal and the colonic organoids of inflammatory bowel disease patients. The observed swelling response to PGE2 appeared to be dependent on the environmental Cl− concentration as well as on the presence of membrane-bound transporters or channels, such as CFTR, NKCC1, KCNQ1 and KCNN4. A panel of inflammatory cytokines completely failed to modify such a PGE2-mediated response, thus collectively indicating that PGE2 functions as a direct and robust mediator of Cl− secretion by human IECs in various mucosal environments.
Establishment of a quantitative screening method to evaluate the swelling response of intestinal organoids
To establish a method by which we can efficiently quantify the swelling of subject organoids in response to a large variety of conditions, we employed a newly developed 3D-scanning system to measure the cross-section area of the organoids (Supplementary Fig. S1). Our 3D-scanning system consists of a light emitting diode (LED)-type white light device positioned above the culture dish and a charge-coupled device (CCD) camera positioned underneath the dish; this camera can acquire a full-scan image of a single plate at a resolution of 4800 dpi within 1 min. First, to test the swelling response of our human intestine-derived organoids, FIS was examined. Throughout the present study, we mainly used intestinal organoids that were established from the uninflamed mucosa of patients with Crohn’s disease (CD). These organoids were maintained under stem/progenitor cell-enriched culture conditions as an in vitro model of crypt cells. The epithelial cell identity and stem/progenitor cell properties of the collected samples from the patients were confirmed by immunostaining for E-cadherin, pan-cytokeratin, Musashi-134 and Ki-67 (Supplementary Fig. S2).
The shape of the organoids was either multilobular or spheroid depending on when the analysis was performed after the last passage; organoids analysed within 2 days post-passage predominantly presented with a spheroidal shape, while those analysed over 7 days post-passage predominantly showed a multilobular shape. These types of shape changes during the routine culture time course were repetitive and do not represent the variability among independent organoids at the same time points. Spheroidal organoids were consistently used for the following analyses using the 3D-scanner system. However, multilobular organoids were used in some of the pulse-chase experiments, as the dynamic change in shape was more demonstrative and clearly confirmed.
Consistent with the former reports32,35, upon the addition of FSK, small intestinal organoids showed a rapid swelling response that continued for at least 60 min (Fig. 1a and Supplementary Movie S1). Further examination for up to 240 min revealed that organoids invariably show a continuous linear response up to 30 min after FSK stimulation (Supplementary Fig. S3). However, at time points longer than 30 min, the response curve showed a highly heterogeneous pattern among the organoids. Some of the organoids presented with a plateau pattern, suggesting that the response reached equilibrium. Other organoids showed a decrease in the response curve, indicating a collapse of the organoids (Supplementary Movie S2). From these preliminary data, we determined that measurements up to 30 min would be appropriate to examine the anion/fluid transport response of the organoids.
Using FIS as a positive control, we next tested if such a swelling response could be quantified by our 3D-scanning system. To optimize the quantification efficiency and accuracy, organoids were subjected to analysis one day after the last passage, as those organoids generally held their cystic shape at this time point. From here, the threshold to recognize the cross-section border of each organoid was optimized (Supplementary Table S1). Then, we confirmed that the optimized threshold setting accurately aligned with the cross-section border of each organoid (Fig. 1b), which would thereby precisely measure the cross-section area. By scanning the organoids before and after FSK addition, we calculated the increase in cross-section area of each organoid (% change in cross-section area as defined in the Material and Methods section) and reported this value as an index of the swelling response. Using the present system, we confirmed that the time-dependent swelling response can be quantitatively monitored at the single organoid level (Fig. 1c). Following the methods used by Dekkers et al.32, we also compared the response of individual wells to evaluate the inter-well difference of FIS (Fig. 1d). The results showed that the % change in cross-section area derived from the total cross-section area of organoids in a single well is highly conserved among the individual wells that were examined under the same conditions.
By running the present system, we could quantify the swelling response at a maximum output of 384 wells per single scan (Supplementary Fig. S1). This makes it possible for us to use the present system for drug response screening as well as determine the dose-response curve of a limited number of test reagents. Thus we tested whether the system could determine the dose-response curve of FSK. Using a human jejunum-derived organoid, we found that the dose-response curve for FIS appears as a standard sigmoid shaped curve, and the log EC50 value was calculated as −7.58 (Fig. 1e).
Series of candidate endogenous mediators of anion/fluid secretion promotes the swelling of human jejunal organoids
To examine the direct effect of various endogenous mediators that may induce anion/fluid secretion from IECs, we tested 6 endogenous mediators to determine if they have the potential to induce the swelling response of organoids equivalent to that of FIS. Our time-lapse imaging showed marked differences among the mediators (Supplementary Movies S3–S8). The two mediators that function through Gs-coupled receptors, PGE2 and VIP, showed a rapid and continuous response that ended as a large overall increase in the cross-section area of the organoids at 60 min after induction (Fig. 2). In contrast, the mediators that function through Gq-coupled receptors, ACh and histamine, showed a slow and limited response, and the increase in the cross-section area of the organoids appeared to be smaller compared to that of either PGE2 or VIP. The addition of bradykinin or serotonin had no clear effect on the induction of organoid swelling.
To confirm the data acquired by time-lapse imaging, we next tried to examine the difference in the dose-response curves of mediator-induced organoids. First, we used a human jejunal organoid to evaluate the direct response of these mediators. The results showed a clear sigmoid-shaped response curve for PGE2 and VIP, while a much lower response profile was observed for ACh and histamine (Fig. 3a). This type of response pattern was also generally conserved in the human colonic organoids derived from the uninflamed mucosa of a patient with ulcerative colitis (UC) (Fig. 3b). Thus, these results showed that PGE2 could induce the swelling of jejunal and colonic organoids at the lowest log EC50 value of the mediators tested. In addition, the dose-response curves and log EC50 values of PGE2 were reproduced and confirmed in another line of jejunal and colonic organoids established from a single CD patient (Supplementary Fig. S4) as well as in organoids established from the intact ileal mucosa of a UC patient (Supplementary Fig. S5), suggesting that our present data may be common among the uninflamed mucosa of IBD patients. Importantly, organoids that were established from the healthy mucosa of non-IBD patients also showed an equivalent response to PGE2 (Fig. 3c). Of note, the EC50 value identified for PGE2 in the jejunal organoids appeared to be 100-fold lower than that of FSK (Fig. 1e). In both the jejunum and the colon, the response to PGE2 also showed the highest values (Fig. 3d,e). Therefore, among the various endogenous mediators of anion/fluid secretion, PGE2 functions as one of the key inducers of anion/fluid secretion due to its direct effect on IECs.
Our organoids consistently and clearly expressed the PGE2 receptor subtypes EP1, EP2 and EP4 (Supplementary Fig. S6). We further examined if other types of prostaglandins, such as PGD2, may have the potential to induce the swelling of human small intestinal organoids (Supplementary Fig. S6). The results showed that PGA2 and PGE1 may have an equivalent potential to induce the swelling of human organoids. However, previous reports have indicated that PGE2 has the highest potency of intestinal fluid secretion among eicosanoids36. Additionally, the local production of PGE2 but neither the production of PGA2 nor PGE1 is clearly upregulated in the inflamed mucosa of IBD patients24,36,37, suggesting that PGE2 may be the dominant eicosanoid mediator of anion/fluid secretion in these patients.
PGE2-induced organoid swelling is dependent on Cl−
Studies have shown that fluid secretion by IECs is mediated by the secretion of anions such as Cl− 4. To identify if the previously observed response induced by PGE2 is mediated by the secretion of Cl−, we examined the response in Cl−-free Ringer’s solution. In Ringer’s solution supplemented with 126.8 mEq of Cl−, the response of jejunal organoids to PGE2 was clearly conserved (Fig. 4a). However, when those organoids were placed in Cl−-free Ringer’s solution, the PGE2-induced swelling response was completely eliminated (Fig. 4b). The abolishment was not due to the loss of cell viability under the Cl−-free environment, as the response was completely restored by adding buffer supplemented with Cl− (Fig. 4b). A time-course experiment lasting up to 90 min using jejunal organoids established from either the uninflamed mucosa of a CD patient (Fig. 4c) or the healthy mucosa of a non-IBD patient (Fig. 4d) further showed that a clear swelling response could be neither induced nor maintained in organoids that were placed in a Cl−-free environment, but the organoids regained their swelling response once they were placed back into Cl−-supplemented Ringer’s solution. Additionally, the dependence on the Cl− concentration was further confirmed by quantification of the response at different levels of Cl− using organoids of both origins (Fig. 4e,f). Thus, these results support the idea that the PGE2-induced organoid swelling is dependent on the induction of Cl− secretion from organoid-based IECs.
Previous reports have indicated that HCO3− may also contribute to the secretory process of IECs35. Thus, we further tested whether HCO3− may play a role in the present swelling response by adding HCO3− to Cl−- and HCO3−-free Ringer’s solution (Supplementary Fig. S7). Consistent with the previous experiment, depletion of both Cl− and HCO3− completely abrogated the swelling response. Supplementation of Cl− almost completely restored the swelling response, whereas supplementation of HCO3− partly restored the swelling response to a minimal extent. Thus we conclude again that the secretory response is predominantly dependent on Cl− secretion, accompanied by a minor contribution of HCO3− secretion.
PGE2-induced organoid swelling is sensitive to inhibitors targeted to CFTR, NKCC1, KCNQ1 and KCNN4
Various transporters and channels that are expressed on the apical and basolateral membranes of IECs constitute the molecular mechanism that mediates anion/fluid secretion by the intestinal epithelium. Therefore, we examined if these molecules are expressed and functional in our organoids. RT-PCR analysis showed that mRNA expression of CFTR, NKCC1, KCNQ1 and KCNN4 are clearly detected in the small intestinal organoids (Fig. 5a). Within these organoids, the expression of NKCC1 and KCNQ1 was confirmed at the protein level, as the immunostaining of those molecules showed clear localization at the basolateral membrane (Fig. 5b).
We next examined whether these membrane-bound channels or transporters contributed to PGE2-induced organoid swelling. A series of inhibitors targeted to CFTR showed a clear inhibitory effect on the PGE2-induced swelling of jejunal organoids (Fig. 5c). Although the inhibitory effect varied among the compounds, addition of glyburide (500 μM) showed a marked abolishment of this response, suggesting that the PGE2-induced Cl− secretion on the apical side might be highly dependent on CFTR. In addition, we found that inhibitors specific for NKCC1, KCNQ1 or KCNN4 can also partially reduce PGE2-induced swelling (Fig. 5c), indicating that Cl− uptake on the basolateral side requires cooperative function of these channels and transporters. A similar effect of the inhibitors targeted to CFTR, the K+ channel or NKCC1 was confirmed not only in the jejunal organoids that were established from the uninflamed mucosa of an IBD patient but also in organoids that were established from the healthy mucosa of a non-IBD patient (Fig. 5d).
Studies have suggested that growth factors such as epidermal growth factor (EGF) may confound the properties of the IECs by dysregulating the expression of anion channels or transporters38. However, depletion of EGF for up to 24 hours did not change the response of PGE2-induced swelling (Supplementary Fig. S8). Thus, our results so far indicate a limited influence of growth factors such as EGF on PGE2-induced swelling.
PGE2-induced organoid swelling cannot be modulated by inflammatory signals
PGE2 is a mediator whose expression is upregulated in different IBDs, such as UC or CD24,39,40. In such an environment, the response of IECs could be modified by the concomitant presence of various pro-inflammatory cytokines41. Therefore, we examined whether the present PGE2-induced organoid swelling could be modified by the presence of inflammatory cytokines. Short-term pretreatment (30 min) of the jejunal organoids with cytokines such as TNF-α, IL-1β, IL-6, IL-22 or IFN-γ alone failed to induce a swelling response (Fig. 6a). When PGE2-induced swelling was tested following the cytokine pretreatment, the response level of both low-dose PGE2 (10−11 M) and high-dose PGE2 (10−9 M) remained completely unchanged (Fig. 6b,c). We further examined the effect of up to 13 major cytokines that are relevant to IBD and found that all the tested cytokines exerted absolutely no effect on PGE2-induced swelling. These results indicate that PGE2 may act as a robust and direct mediator of anion/fluid secretion from IECs, which cannot be affected by other pro-inflammatory cytokines. Additionally, the results raise the possibility that the local levels of PGE2 may constitute one of the components in the pathophysiology of IBD-associated diarrhoea42,43.
In the present study, we established a modified method to quantitatively evaluate the swelling of human intestinal organoids. Our method employed a 3D-scanner that can quantify the swelling response of a large number of samples simultaneously in a 96-well plate format. The current method did not use fluorescent staining to detect the inner rim of the organoids but instead used an auto-focused phase-contrast image of a single well. Using the ratio of cross-section area as an index of swelling, we acquired experimental results of FIS treatment that are consistent with previous reports32,35,44, using human intestinal organoids. In the experiments analysing FIS, all the organoids responded without exception and resulted in a complete response of the subject organoids (Fig. 1c). However, if we included other replicates of the experiment, 1 out of 63 subject organoids failed to respond to forskolin (1.53% of total organoids). Such a low rate of nonresponsive organoids compared to that from a previous report32, may be due to differences in the culture conditions, passage methods or background characteristics of the source from which the organoids were derived.
The present method may be further applied to test the swelling response of a wide variety of compounds or natural ligands and to efficiently evaluate their potential to induce or otherwise inhibit the anion/fluid secretion of IECs. Among the examined endogenous secretagogues, VIP and PGE2 showed a clear and extended response, whereas ACh, histamine, bradykinin and serotonin showed a somewhat weak and unsustained response. This profound swelling response after PGE2 addition was generally conserved between organoids established from the non-inflamed region of IBD patients and organoids established from the healthy mucosa of non-IBD patients. The differential response among the examined secretagogues may arise from the difference in the intracellular signalling of the corresponding receptors. The receptors for VIP and PGE2 are Gs-coupled receptors both mediate cAMP intracellular signalling, while the receptors for ACh, histamine, bradykinin or serotonin are Gq-coupled receptors, which mediate Ca2+-based intracellular signalling1. Gq-coupled receptors undergo tachyphylaxis and thus cannot sustain their response under a continuous ligand presence in vitro. Another possibility is the difference in the expression levels of the corresponding receptors. Regarding the response to PGE2, EP2 and EP4 may be the dominant receptors in IECs45,46. Clear expression of both EP2 and EP4 was consistently found in our organoids (Supplementary Fig. S6). However, further study using a set of EP agonists and antagonists may reveal the receptor that is responsible for the observed swelling response. Additionally, our finding that PGE2-induced swelling can be completely nullified by removing all Cl− from the buffer indicates the great dependency of this activity on Cl− uptake and secretion in the basal-to-apical direction.
The attempts of our study to identify the transporters and channels that are responsible for the PGE2-induced swelling showed that CFTR might be the dominant transporter on the apical membrane. Among the CFTR inhibitors, glyburide showed the most significant inhibitory effect on PGE2-induced swelling (Fig. 5c,d). Glyburide is a compound classified as a second-generation sulfonylurea antidiabetic drug. It can act as an inhibitor of ATP-sensitive potassium channels (Kir6.1 and Kir6.2, KATP) in pancreatic β-cells in addition to its activity on CFTR according to the IUPHAR database. Although the expression of neither Kir6.1 nor Kir6.2 has been identified in human IECs, it remains possible that glyburide inhibits swelling of the organoids through the inhibition of ATP-sensitive potassium channels such as Kir6.1 or Kit6.1 in addition to its activity on CFTR.
In addition, the inhibitor study showed that NKCC1, KCNQ1 and KCNN4 might contribute to the response on the basolateral membrane. Both NKCC1 and KCNQ1 are exclusively expressed in crypt-resident cells but not in those cells residing at the villi35,47. Thus our results suggest that cooperation of these transporters is induced specifically in crypt IECs upon local secretion of PGE2. The possible involvement of other channels, such as ClC-2 or CaCCs48 remains to be elucidated. However, the existence and the role of CaCCs in the intestinal epithelia still remains controversial49, and these channels may have a more dominant role in intestinal motility50. Additionally, the residual secretion observed in each inhibitor study suggests the possibility that the partial compensation of off-target transporters/channels or the reciprocal activation of an as of yet unknown transporter/channel system is induced in IECs. Although the inhibitors were tested at a concentration far above their IC50, a possibility remains that the residual secretion is due to their partial effect on the target channel or transporter.
Surprisingly, the response to PGE2 was robust and minimally influenced by short-term treatments with inflammatory cytokines. With regard to the pathophysiology of inflammatory diarrhoea, transport of Cl− may represent a partial component, as other factors such as disruption of the mucosal integrity or an increase of exudates arising from the damaged mucosa may also constitute the major components of inflammatory diarrhoea. However, expression of PGE2 is upregulated in the inflammatory lesions of UC and CD patients, where other inflammatory cytokines are also abundant51. Thus our present results suggest the possibility that the local PGE2 concentration may determine the local secretory fluid response of IECs without the involvement of other inflammatory cytokines and thereby partially contribute to the pathophysiology of inflammatory diarrhoea. Consistently, none of the cytokines tested in the present study was able to induce organoid swelling. Further studies using organoids established from the diseased mucosa of IBD patients may provide insights to the role of PGE2 in the pathophysiology of IBD.
The current organoid system may have some superior points compared to other existing systems used for transport measurement52. For example, biopsies mounted in Ussing chambers could be used to perform experiments that mimic physiological conditions. Such a system may be better suited for electrophysiological studies to discriminate the contribution of each anion and to evaluate the overall effect of the mucosa including cells other than IECs. Additionally, these chambers can be easily applied to analyse the possible differences in responses at the apical or basolateral lumen. However, it may be better to use the organoid system when one wants to examine the direct effect of the mediators to epithelial cells, and wish to employ the volume of water secretion as a readout of the response. Additionally, the use of organoids has the following advantages: (1) the cultures are viable for a longer period with proper maintenance, (2) they can be analysed under differentiated or undifferentiated culture conditions35, and (3) they can undergo semi-high throughput screening (as shown in the present study). Currently it is difficult to access the inner lumen of organoids, which makes manipulating the IECs from the apical lumen one of the major disadvantages of using the organoids.
Finally, we would like to emphasize the caveat of using the current organoid swelling model to evaluate fluid secretion by IECs. First, our present condition mainly reflects the response of crypt cells and minimally accounts for the contribution of villus cells. Second, the quantitative evaluation was carefully restricted to regularly shaped organoids, as the current assay estimates the influx fluid volume from the representative cross-section area. Third, the possibility of fluid leakage through a weakened or fragile section of the epithelial monolayer should be carefully considered, especially when an inhibitory effect is observed.
Material and Methods
Establishment and culture of human intestinal organoids
Human intestinal biopsy specimens were obtained from patients who underwent enteroscopic examination for the evaluation of diseases such as occult bleeding, irritable bowel syndrome, Crohn’s disease and ulcerative colitis. Two or three biopsies were taken from an endoscopically normal region. Surgical specimens of ulcerative colitis patients were also collected to establish organoids. The study was approved by the Ethics Committee of Tokyo Medical and Dental University and the Yokohama Municipal Citizens Hospital, and written informed consent was obtained from each patient. A total of 38 lines of small intestinal (non-IBD origin, n = 3; IBD origin, n = 20) or colonic organoids (non-IBD origin, n = 4; IBD origin, n = 11) were established from 27 patients. All the experiments were conducted in accordance with the approved guidelines. Throughout the present study, unless otherwise indicated, we mainly used intestinal organoids that were established from the uninflamed mucosa of patients with CD. Additionally, we never used organoids established from endoscopically active diseased mucosa of IBD patients.
Isolation of the crypts and the subsequent establishment of intestinal organoids were performed as previously described28,53. Briefly, crypts were collected by rigorously shaking biopsy specimens in 2.5 mM EDTA. Isolated crypts were embedded in 15 μl of Matrigel at a density of 20–30 crypts per well and placed in either a 24-well or 48-well culture dish. Those crypts were maintained in DMEM-based culture medium (Advanced-DMEM, Invitrogen, California, USA) supplemented with recombinant human R-spondin-1 (1 μg/ml, R&D Systems, Minneapolis, USA), recombinant human Wnt-3a (300 ng/ml, R&D Systems, Minneapolis, USA), recombinant human Noggin (100 ng/ml, R&D Systems, Minneapolis, USA), recombinant human EGF (50 ng/ml, PeproTech, USA), Y-27632 (10 μM, Sigma-Aldrich Japan, Tokyo, Japan), A83-01 (500 nM, Sigma-Aldrich Japan, Tokyo, Japan) and SB202190 (10 μM, Sigma-Aldrich Japan, Tokyo, Japan). These culture conditions maintained the organoids in an undifferentiated state, and thus allowed us to define the secretory function of human IECs using those organoids.
Cl−-free Ringer’s solution and HCO3−-supplemented Ringer’s solution were prepared as previously described54,55. The composition of each solution is shown in detail in Supplementary Table S2. The chemicals and reagents that were used in the present study were as follows: PGA2, PGD2, PGE1, PGE2, PGF2α, PGI2 and bumetanide (Cayman Chemical, Michigan, USA); VIP, ACh, histamine, bradykinin and serotonin hydrochloride (Sigma-Aldrich Japan, Tokyo, Japan); glyburide, GlyH-101, CFTRinh172 and PPQ-102 (Santa Cruz, Texas, USA); XE 991 dihydrochloride, 4-AP, HMR 1556 and TRAM 34 (Tocris Bioscience, Bristol, UK); recombinant human IL-10, IL-12, IL-17, IL-33 and TL1A/TNFSF15 (R&D Systems, Minneapolis, USA); and recombinant human TNF-α, IFN-α, IFN-γ, IL-1β, IL-4, IL-6, IL-22 and IL-24 (PeproTech, Rocky Hill, USA). The phase-contrast images of cultured cells were collected by using a microscope (BZ-X700, KEYENCE, Tokyo, Japan). The microscope is an inverted type microscope system dedicated to bright-field and fluorescence imaging and is equipped with a 3.7 W LED and 80 W metal halide lamp. Objective lenses from the Nikon CFI60 series were used for the present study (CFI Plan Apo λ 4x, NA 0.20; CFI Plan Apo λ 10x, NA 0.45; CFI Plan Apo λ 20x, NA 0.75; CFI Plan Apo λ 40x, NA 0.95).
Quantification of organoid swelling
The quantification of organoid swelling was performed using a 3D-Scanner (Cell3 iMager, Screen Holdings, Kyoto, Japan). Organoids were seeded into a 96-well plate for the 3D-scanning with 2 μl of Matrigel and 100 μl of complete culture medium. On the next passage day, scanning was performed both before and 30 min after the addition of the reagent that was subjected for analysis. Upon scanning, an image was acquired by the auto-focus (AF) mode. The total cross-section area of the recognized organoids was automatically determined by setting the analysis parameters as summarized in Supplementary Table S1. Briefly, both the “Edge detection” mode and the “Include high light area” mode were the most critical settings for the present analysis (Supplementary Fig. S1). The index of organoid swelling acquired at the specified time in minutes (% change in cross-section area) was calculated by the following formula:
Time-lapse imaging of the organoids was performed by using the EVOS-FL cell imaging system (Thermo Fisher Scientific, Waltham, MA, USA). Cells were maintained in a stage top incubator (Tokai Hit, Shizuoka, Japan) at 5% CO2 and 37 °C. Phase-contrast images were acquired at 1-min intervals for up to 60 min and were processed into a video file using the EVOS-FL system software (Thermo Fisher Scientific, Waltham, MA, USA).
Semi-quantitative RT-PCR analysis was done as previously described56. The PCR reaction was run by using a Veriti thermal cycler system (Applied Biosystems, Waltham, MA, USA). The primer sequences for human β-actin have been previously described56. Primer sequences for other genes are as follows: EP1, 5-CTTCGGCCTCCACCTTCTTT-3 (sense) and 5-GCCACCAACACCAGCATTG-3 (antisense); EP2, 5-GCTCCTTGCCTTTCACGATTT-3 (sense) and 5-AGGATGGCAAAGACCCAAGG-3 (antisense); EP3, 5-CTTCGCATAACTGGGGCAAC-3 (sense) and 5-TCTCCGTGTGTGTCTTGCAG-3 (antisense); EP4, 5-CGCTCGTGGTGCGAGTATT-3 (sense) and 5-CCCGCCAATGCGGCAG-3 (antisense); CFTR, 5-CGCCCGAGAGACCATGC-3 (sense) and 5-CAGCTCTCTATCCCATTCTCTTT-3 (antisense); NKCC1, 5-ACACACAAAGTTGAGGAAGAGGA-3 (sense) and 5-GGCACAATAGGGCCTTTGGA-3 (antisense); KCNQ1, 5-CGTCTCCATCTACAGCACGC-3 (sense) and 5-CAGGACGATGAGGAAGACGG-3 (antisense); and KCNN4, 5-TGCACGATCAGCATTTCCAC-3 (sense) and 5-GTCGGTCATGAACAGCTGGA-3 (antisense). Semi-quantitative analysis was performed by the following numbers of PCR cycles: 34 cycles for EP1, 32 cycles for EP2, 34 cycles for EP3, 32 cycles for EP4, 32 cycles for CFTR, 28 cycles for NKCC-1, 31 cycles for KCNQ-1, 28 cycles for KCNN-4 and 20 cycles for β-actin. The amplified products were separated by electrophoresis in a 3% agarose gel and visualized by ethidium bromide staining. Stained images were then acquired by using the ChemiDoc imaging system (Bio-Rad Laboratories, Hercules, CA, USA).
Immunostaining of the organoids was performed as previously described29. The following primary antibodies were used in the present study: rabbit anti-human KCNQ1 (H-130) antibody (1:300, Santa Cruz, Texas, USA), mouse anti-human SLC12A2/NKCC1 (5H7) antibody (1:100, LSBio, Washington, USA), mouse anti-human E-cadherin (HECD-1) antibody (1:100, Takara, Tokyo, Japan), mouse anti-human pan-cytokeratin antibody (1:50, clone AE1/AE3, DAKO, Glostrup, Denmark), rat anti-human Musashi-1 antibody (1:1000, 14H1, eBioscience) and mouse anti-human Ki-67 antibody (1:50, DAKO, Glostrup, Denmark). The primary antibodies were visualized with either Alexa Fluor® 488-conjugated secondary antibodies (Thermo Fisher Scientific, California, USA) or FITC-conjugated tyramides (Perkin Elmer, Waltham, USA). Signal amplification was required to detect NKCC1, KCNQ1 and Musashi-1. Images were collected by using an epifluorescent microscope (BZ-X700, KEYENCE, Tokyo, Japan).
Unless otherwise indicated, the data were confirmed by at least three independent experiments, each consisting of three replicates acquired from three independent culture wells. Data are shown as the mean ± SEM of three independent wells. The significance of these data were analysed by using an unpaired two-sided Student’s t-test. The significance was defined as P < 0.05 (*). The EC50 values were calculated using GraphPad Prism (GraphPad Software, Inc., California, USA).
How to cite this article: Fujii, S. et al. PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells. Sci. Rep. 6, 36795; doi: 10.1038/srep36795 (2016).
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This study was supported by MEXT/JSPS KAKENHI grant number 25293170 (to RO, KT and TN), grant number 23102003 (to RO and TN), grant number 15K15286 (to RO), and grant number 226221307 (to KT, TN and MW); the Research Center Network Program for Realization of Regenerative Medicine from the Japan Science and Technology Agency (JST) and Japan Agency for Medical Research and Development, AMED (to RO, TN, KT and MW); the Practical Research Project for Rare/Intractable Diseases from Japan Agency for Medical Research and Development, AMED (to KT and MW). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.