Like the majority of benthic invertebrates, the blue mussel Mytilus edulis has a bentho-pelagic cycle with its larval settlement being a complex phenomenon involving numerous factors. Among these factors, underwater noise and pelagic trophic conditions have been weakly studied in previous researches. Under laboratory conditions, we tested the hypothesis that picoplankton assimilation by the pediveliger blue mussel larvae acts as a food cue that interacts with anthropic underwater sound to stimulate settlement. We used 13C-labeling microalgae to validate the assimilation of different picoplankton species in the tissues of pediveliger larvae. Our results clearly confirm our hypothesis with a significant synergic effect of these two factors. However, only the picoeukaryotes strains assimilated by larvae stimulated the settlement, whereas the non-ingested picocyanobacteria did not. Similar positive responses were observed with underwater sound characterized by low frequency vessel noises. The combination of both factors (trophic and vessel noise) drastically increased the mussel settlement by an order of 4 compared to the control (without picoplankton and noise). Settlement levels ranged from 16.5 to 67% in 67 h.
The blue mussel, Mytilus edulis, like the majority of temperate marine benthic mollusks, produces free swimming planktotrophic larval stages, which feed, grow and disperse via water currents until the competent pediveliger stage is physiologically capable of settling and metamorphosing onto a substrate1. However, pediveliger larva can extend the duration of its planktonic stages to significantly increase their size at metamorphosis2,3,4,5,6,7. Martel et al.4 observed that delayed metamorphosis can induce 47.8% of additional larval shell growth representing a 322% difference in larval body mass at settlement. Among the wide range of settlement cues interacting in the larval settlement behavior, the ambient underwater sound is the most probable for guiding onshore orientation by pelagic larvae of numerous common coastal species such as fish8,9, crustacean10,11 and coral larvae12. Moreover, ambient underwater sound from reefs has been shown to initiate settlement behavior and decrease metamorphosis initiation time on benthic dwelling larval stages in several crab species13,14. A recent study focusing on mytilid Perna canaliculus showed that larval settlement was significantly faster when exposed to the underwater noise produced by a passenger and freight ferry15. Over the last 50 years, vessels contributed to a 32-fold increase in the low frequency noise present in some parts of the ocean16. Vessel noise is generated by the operating high energy noisy propellers, gears and diesel generators17. The underwater sound produced by vessels is biologically important for settlement stage larvae, particularly species involved in fouling and bioinvasion such as mussels, ascidians and barnacles15,18,19.
In their perspective section, Wilkens et al.15 suggested that “the relative importance of underwater sound as a settlement cue or the potential for underwater sound to act in a synergistic manner with other settlement cues is unknown”. Field studies of Toupoint et al.6,20 suggested that picoplankton abundance acts as a trophic settlement trigger for mussel larvae and that these pelagic cues are dominant over other cues as substrate features including biofilms21. Pelagic picophytoplankton includes prokaryotes and eukaryotes that are classically defined by the 0.2–2 μm size range. Prokaryotic species belong mostly to cyanobacteria of the genus Prochlorococcus22 and Synechococcus23. The latter is a small unicellular cyanobacterium, 1 μm in diameter, abundant in temperate and tropical oceans22 and contributing to a substantial proportion of marine primary production24,25. Previous studies showed that when nanoplankton biomass is limited, invertebrates’ larvae may exert considerable grazing pressure on Synechococcus spp.25,26.
In the present study, we propose to analyze the synergistic or antagonistic effects of two interacting factors on larval settlement of the blue mussel (Mytilus edulis): i) the anthropic underwater sound and ii) the contribution of picoplankton (Nannochloropsis oculata and Synechococcus sp.). Experiment using 13C-labeled picoplankton was performed in parallel to test whether the picoeukaryotic species were ingested and assimilated by pediveliger larvae. Fatty acid trophic markers methods were used to validate the microalgal assimilation as mussels are not able to biosynthesizing highly polyunsaturated fatty acids27. We tested the hypothesis that picoplankton cells, inducing a nutritional cue, are assimilated by blue mussel pediveliger larvae and together with specific underwater sound, can stimulate settlement.
Underwater sound recordings
Replayed vessel noise in the different jars of Aquarium 1 was homogeneous and measured at 127 ± 3 dB re 1 μPa between 100 and 1,000 Hz corresponding to the in situ recorded source signal (Fig. 1). In the two others aquariums, corresponding to treatment without sound emission, the sounds levels differed from the Aquarium 1 (Fig. 1 and Table 1). The sound levels were slightly higher than the conditions before experiment (Table 1) but remained consistent with rough natural conditions defined by Wenz’ formula28 (Fig. 1).
Vessel noise had an interactive significant with N. oculata trophic treatment (F1, 16 = 6.682, p = 0.02). Thus, adding N. oculata induced a significant increase of 6.6% of larval settlement rate in the silent aquarium, vessel sounds alone increased settlement by 27% in aquarium without N. oculata and the most intense effect was observed with the combination of trophic and vessel noises (increase of 50.7% comparatively to control condition; Fig. 2). By contrast to N. oculata, the addition of Synechococcus sp., did not significantly affect the mussel settlement rate at a mean of 10.2 ± 1.8% similar to control (t4 = 0.556, p = 0.608).
Larval Size and Metamorphosis
Neither the occurrence of vessels sounds nor N. oculata differentiate swimming larvae size (Table 2, vessel: F1, 317 = 1.05, p = 0.307; trophic: F1, 317 = 0.003, p = 0.955; interaction: F1, 317 = 0.13, p = 0.723). However there was a significant interaction between sound and trophic treatments against settlers size (F1, 249 = 4.45, p = 0.033) resulting in sizes 5% smaller in condition with vessel noises than other treatments (Table 2, p < 0.03). Metamorphosis was determined by the presence of a prodissoconch shell (Fig. 3). In swimming larvae, the mean metamorphosis success was very low or null for all treatments (0.9 ± 0.7%) comparatively to settlers larvae (45.7 ± 5.3%), suggesting that the larvae settled before engaging in the metamorphosis process. In settlers, vessels sounds and trophic treatments had no significant effect in the observed percentage of metamorphosis (Table 2, vessel: F1, 19 = 0.90, p = 0.357; trophic: F1, 19 = 1.17, p = 0.295; interaction: F1, 19 = 0.23, p = 0.639).
Lipids of the two picoplankton species treatments (N. oculata and Synechococcus sp.) were strongly enriched in 13C by our culture method as the %13C of each FA analysed were over 87% (Table 3). Results of the Permanova analysis showed significant differences (Pseudo-F(3–9) = 1698, p = 0.0002) between the four treatment (N. oculata, Synechococcus sp. and larvae fed with each picoplankton species) in the 13C enrichment for dominant FA (Table 3). The pair-wise tests indicate that all comparisons were significantly different (p < 0.001), except for N. oculata and Synechococcus sp. showing similar levels of 13C enrichment (t = 3.961, p = 0.055). Larvae fed with 13C N. oculata showed FA enrichment in their polar lipids largely over the level observed in larvae fed with 13C Synechococcus sp. with enrichment levels exceeding 21%, except for the 22:2 NMI with 13.5%. For larvae fed with 13C Synechococcus sp., the labeling levels were close to the natural levels of 1.1%.
Our results clearly confirm our hypothesis that picoplankton assimilation by blue mussel pediveliger larvae acts as food cues that interacts, with some degree of synergy, with anthropic underwater sound to stimulate larval settlement. Toupoint et al.6 demonstrated in natural conditions that Mytilus edulis larval settlement was enhanced in the presence of picoplankton, but could not determine whether the picoplankton acted as a chemical cue or was ingested and assimilated by the larvae. Our experimental study clearly confirms that only picoplankton species involved in the diet of pediveliger larvae act as a trophic settlement trigger (TST). Moreover, in accordance with a Wilkens et al.15 study on Perna canaliculus, we also demonstrated the positive response of vessel noise on M. edulis settlement. Although previously hypothesized by Wilkens et al.15, we show here for the first time that anthropic underwater noise act in a synergistic manner to amplify the TST’s impact: the combine synergic effects of these two factors greatly increased the settlement success up to 70% in less than 67 h. Such high settlement levels are rare in laboratories studies on the blue mussel. For instance, the settlement success of competent M. edulis larvae (>50% pediveliger) in a flow-through system with the best suitable hydrodynamic treatment after 7 days, was less than 20%29. In laboratory static conditions without filamentous substrate (as in the present study), no settlement success of M. edulis larvae30. Larval settlement of M. edulis and bivalves in general, is complex and involves a wide range of interacting settlement cues like surface wettability and microtopography31,32, presence of conspecifics33, light regimes33,34, chemical cues32,35,36, biofilms20,37 and hydrodynamic conditions29,38. When tested in various combinations, some of these factors have shown greater success than others in supporting larval settlement, such as pelagic trophic condition compared to biofilm development20, while other combinations, such as filamentous substratum and turbulence30 have shown to be synergic. Our results show that the combination of anthropic vessel sound and trophic condition increased settlement success up to 51% than the control. In the present study, the anthropic and natural underwater sounds were recorded from one of the important suspension mussel culture areas of Eastern Canada, where about 38% of the St Peter’s Bay total surface area is dedicated to mussel farming. The natural underwater ambient sound was measured at levels inferior to the two aquariums corresponding to the conditions “silent” in our experiment. Ambient soundscape cultured suspension mussels differ clearly from surf-zones of rocky shores and biogenic reefs, which is the natural habitat of M. edulis39,40. Such habitats produce natural underwater sounds ranging from 88 to 145 dB re 1 μPa RMS including the sound level of vessel noise (130 dB re 1 μPa). Although the ambient underwater sound is now recognized as an important factor in guidance for the larvae, the possible sensory mechanism for the mussel larvae to detect sound is still unknown.
Anthropic underwater noise increases the settlement of mussel pediveliger larvae but decreases the size of the settler with potential cascading ecological impacts. Indeed, as the body mass follows the usual cubic progression in relation to shell length, a 5% smaller size could represent a much greater decrease of larval body mass. With marine benthic invertebrates, the size of the first post-larval stage is recognized as a key life history parameter, which constitutes a significant biological attribute in the ecology, ethology and evolutionary biology of the species41,42,43,44,45. High post-larval mortality could reach 90% and is mostly related to predation, stress tolerance sensitivity, hydrodynamics condition and competition46,47,48.
Considering the results of isotopic labeling experiments, we showed that the fatty acids (FA) of Synechococcus spp. were weakly assimilated by the larvae, contrasting with the species N. oculata, as demonstrated by the very low level of enriched FA in the lipid polar fraction. Polar lipids are composed mainly of different phospholipid classes that are part of the cell membrane of bivalve larvae49. Nonetheless, our results clearly show that N. oculata was ingested and assimilated by pediveliger larvae: the 20 % increase of 13C enrichment in the polar FA of larvae fed with labeled N. oculata indicates that such microalga provided carbon in the cell membrane of pediveliger larvae during 72 h in experimental conditions. Moreover, the presence of 13C enriched 22:2 non methylene-interrupted (NMI) in the larvae, absent from the microalgae, suggest a biosynthesis of new FA in complement to the direct transfers from specific FA from N. oculata to polar lipids This NMI FA biosynthesis by mollusk have already been demonstrated in several bivalve species, including M. edulis50,51,52,53. These NMI are biosynthesized by the use of 16:1ω-7 and 18:1ω-9 as precursors then elongated to 20:1ω-7 and 20:1ω-9, desaturated into 20:2 and finally elongated in 22:253. As mussels are able to synthesize de novo this NMI FA, such result gives additional evidence of both ingestion and assimilation of N. oculata by M. edulis larvae. With contrast to the N. oculata results, the fact that Synechococcus spp. did not contribute to the larval nutrition could be related to its smaller size (<1 μm) compared to N. oculata (around 2 μm). Hence, we suggest that the role of the trophic settlement trigger should be associated only to picoplankton species, most abundant in marine ecosystems54,55,56, that contribute to the nutrition of mussels’ larvae.
Picoplankton can reach very high concentrations and dominate the phytoplankton biomass in relatively nutrient rich coastal systems54,55,56. Moreover, since the last decade, the contribution of small-size cells to phytoplankton community has increased57,58. In the context of future predicted climate conditions, the biomass of dominant picoplankton species will more contribute to the primary production and their relative weight will change59. In parallel, future marine shipping and associated underwater noise will intensify, especially in low impacted systems as in the Arctic Ocean60. Synergetic effects between noise and picoplankton combined with global predicted increased levels should therefore deeply affect the mussel’s recruitment patterns in coastal areas.
Recordings of underwater sound were made at St Peter’s Bay on Prince Edward Island (Canada, 46°25.963 N; 62°39.914 W), a major site of suspension mussel aquaculture, using 100-m longlines, with a calibrated hydrophone (High Tech, Inc., Mississippi, USA, HTI-99-HF: sensitivity −169.7 dB re 1 V/μPa; frequency range 2 Hz to 125 kHz flat response) installed at the base of the mussel line near the anchor (25 cm from the bottom) in the middle of the farm. The output was captured with a calibrated underwater acoustic recorder (RTSYS-Marine Technologies, France, EA-SDA14, 156 kHz, 24-bit resolution). The recording lasted 24 hours and was collected on July 5 to 6, 2013. To obtain a sound sequence with anthropic noise, the farmer’s boat (D & H Boatbuilding hull of 11 meters long) equipped with a diesel motor (Cummins 300 hp C series) was passed three times at a slow speed just above the experimental longline. All recordings were conducted in near calm conditions (0.2 m wave height and 3.8 m s−1 wind speed, http://climat.meteo.gc.ca/). Digital recordings were transferred to a PC to enable spectral composition and source sound level determination using the MATLAB (The MathWorks, Inc.) software with customized written codes for post-recording sounds analysis. From the recordings, a sequence lasting 30 s was selected corresponding to “vessel noise” at maximum sound intensity. Moreover, a sequence of 30 s per hour was analyzed to evaluate the ambient underwater noise without anthropic noise.
Two years-old mature mussels collected from St Peter’s Bay were conditioned to spawn in the laboratory61 and the larvae were reared62. Introduction and transfer permits from Fisheries and Oceans Canada were obtained and our study did not involve endangered or protected species. Briefly, fertilized eggs (66.4 ± 7.7 μm) from 20 males and 20 females were reared at 20 °C and 28 PSU salinity in a final experimental concentration of 20 larvae ml−1 in 150 l of filtered seawater (1 μm pore-size). Two upwelling flow-through systems of 0.1 l min−1 with natural light period (16:8 h) were used. Larvae were fed continuously at 30 cell μl−1 with a 1:1:1 mixture of Pavlova lutheri (CCMP 1325), T-isochrysis lutea (CCMP 463) and Chaetoceros muelleri (CCMP 1316), named after PTC mixture. Microalgae were obtained from the National Center for Marine Algae and Microbiota, NCMA (Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, USA) and cultured in seawater enriched with f/2 medium at 20 °C63. Survival rate was 52.2 ± 2.5% until 90% of population was at the pediveliger stage and the daily growth was 10.2 ± 0.3 μm d−1.
Larval settlement experiment
For the experiment, 40-l aquariums were used, each one containing 30 l of water and ten 250-ml individual jars placed on a platform at 18.5 cm from the aquarium bottom to keep jars rims 1 cm above the surface. Aquariums were used to maintain constant water temperature at 20 °C (±1 °C) monitored by probes (Onset Hobo Water Temp Pro V2 Data logger U22-001), with a natural light period (16:8 h). Each aquarium corresponded to an acoustic treatment and jars to a trophic treatment with 5 replicates per condition randomly placed on the platform. At the start of the experiment, 1 pediveliger larvae ml−1 were introduced in each jar filled with 240 ml of 1 μm filtered and UV treated seawater.
The experiment consisted of two aquariums for sound treatments, i.e. presence and absence of ‘vessel noise’”, where presence or absence of Nannochloropsis oculata (CCMP 525) was included. A third similar aquarium, without sound treatment, has been added to evaluate the presence/absence effect of a second species of picoplankton Synechococcus sp. (CCMP 1333). The jars corresponding to control for trophic treatments contained PTC mixture (1:1:1) at a total concentration of 36 cells μl−1. Concentrations in jars with N. oculata and Synechococcus sp. were adjusted to maintain the similar algae biomass than control estimated by dry mass on GF/F filter rinsed with ammonium formate (6%). Consequently, concentrations were 75 cells μl−1 for N. oculata or 150 cells μl−1 for Synechococcus sp. Larvae were left undisturbed for 67 hours. Each jar was lightly rinsed twice over a 200 μm sieve to collect unfixed larvae and the remainder of the jar was gently brushed to collect settled larvae and preserved in 10% formaldehyde. Swimming and settled larvae were counted under binocular microscope using Dollfus counting cell and size was determined using ImageJ, free image processing software, obtained with an upright Olympus BX-41 associated to high resolution video camera (Evolution VF; Media Cybernetics).
Sound emissions were made using an underwater loud speaker (AQUA 30, DNH, 8 Ohms, 80–20,000 Hz) associated with an amplifier (Plug & Play 12 W) connected to a PC that continuously replayed vessel noise. Sound recording calibrations under experimental conditions were made using a digital recorder (Song Meter SM2+, Wildlife acoustics) connected to a calibrated hydrophone (HTI-96 MIN, High Tech, Inc.). The source was located in front of the platform. Consequently, the first row of jars was located 6 cm from the center of the source while the last was 32 cm away. The multiple reflections off the sides of the aquariums produced homogeneous sound conditions (SEM: ±1.5 dB) over the jars, which was confirmed by sound measurements performed in each jar prior the experiment. Correction function was calculated from 30 s recordings of a calibrated sound done in each jar and applied to the vessel noise to replicate as best as possible the shape of the in situ spectrum of the vessel noise. By varying the gain of the amplifier, the intensity was adjusted to match to natural conditions (Sound Level SL: 130 dB re 1 μPa between 100 and 10,000 Hz). Two recordings were also made in adjacent basins to check “silent” conditions (located at 15 cm and 60 cm away).
Picoplankton assimilation experiment
N. oculata and Synechococcus sp. cultures were supplemented with 1 mM sodium [13C] bicarbonate (NaH13CO3, 99%) (Cambridge Isotope Laboratories, MA, USA) then transferred into three sealed 2.8 l polyethylene Erlenmeyer (Nalgen, ThermoScientific, US) and purged with nitrogen gas to eliminate atmospheric CO2. O2 generated during culture growth was removed daily and cultures were maintained at 22.5 °C with continuous orbital agitation (120 rpm) and illumination (100 μE m−2 s−1, Multitron II incubator, Infors-HT, Switzerland). To test 13C enrichment, 15 ml of microalgae culture were stored at −80 °C. These enriched microalgae cultures were introduced in 2.8 l Erlenmeyer, three with a unique diet of N. oculata (75 cells μl−1) and three with Synechococcus sp. (150 cells μl−1) distributed at similar biomass. In each Erlenmeyer, 20,000 pediveliger mussel larvae were added. The six Erlenmeyer were placed under natural photoperiod for 67 h. In parallel, triplicate of Erlenmeyer containing 20,000 larvae were kept as control of non-labeled and not fed larvae. At the end of the experiment, the solution of each Erlenmeyer was then filtered on glass-fiber filters GF/C 47 mm and rinsed well with distilled water to eliminate food. Larvae were gently collected by scraping the filter with a scalpel and stored at −80 °C.
Fatty acids labeling analyses
Assimilation of microalgae was determined through the analyses of isotopic labeling levels of fatty acids (FA) in the food (picoplankton culture) and in the larvae fed with these species64,65. Samples were lyophilized, weighed and lipids extracted by a modified Folch procedure66 as described in Parrish et al.67 and separated into neutral (including triglycerides, free FA and sterols) and polar (mainly phospholipids) fractions68. Polar fractions were hydrolyzed and the extracted FA were analyzed with a modified (see supplementary material) liquid chromatography mass spectrometry method69 using Fourier transform detection (LC-FT-MS, Orbitrap, XL Discovery, Thermo Scientific) in negative modes. The calculation of the percentage of isotope label for a given fatty acid is based on the ratio of the peak area of each isotopomer relative to the sum of all possible isotopomer signals using similar calculation method developed by Leblanc et al. for amino acids65.
Hereafter, treatments will be identified as: S+/− for sound treatment differentiation as well as Nanno+/− and Syneco+/− for trophic treatment with N. oculata and Synechococcus sp., respectively. Data are given as mean± the standard error of the mean (SEM). The settlement data are expressed in percentage of the larvae attached to the total number of larvae in each jar. The metamorphosis data are expressed in percentage of the larvae in prodissoconch II stage to the total number of larvae in each jar. Differences in the larval density, settlement percentage, larvae size and percentage of metamorphosis among treatments were compared using one or two way ANOVA or with a Student t-test when only two mean were compared after verification of normality and equality of variance (by Shapiro-Wilk test). Post-hoc comparisons (Tukey’s pairwise multiple comparison procedure; α = 0.05) were performed to test for any differences among individual treatments. All analyses were performed using software Systat V12.02. Enrichment level of different the dominant FA of picoplankton cultures and larvae fed with these picoplankton have been analyzed by permutational multivariate analysis of variance (PERMANOVA) using PRIMER v6.1.1270 with PERMANOVA+v1.0.271. Homogeneity was evaluated using the permutation analysis of multivariate dispersion (PERMDISP) routine and pair-wise multiple comparison tests were used to identify differences among factors.
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We are especially thankful Mathieu Babin for the isotopic analyses and Stephen Fortune for access to his longlines and boat charter services. We would like to thank Carla Hicks for her manuscript review and to anonymous reviewer for their comments which helped improving the quality of our manuscript. Financial support was provided by the National Sciences and Engineering Research Council of Canada (NSERC-Discovery Grant to RT 299100), the French National Research Agency (MERCALME, ANR-12-ASTR-0021-03), the MNHN for a delegation of 3 years to F. Olivier at the ISMER-UQAR and BeBEST (International laboratory in Benthic Ecology, UQAR-UBO-CNRS).
The authors declare no competing financial interests.
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Jolivet, A., Tremblay, R., Olivier, F. et al. Validation of trophic and anthropic underwater noise as settlement trigger in blue mussels. Sci Rep 6, 33829 (2016). https://doi.org/10.1038/srep33829
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