Abstract
The food-poisoning bacterium Clostridium perfringens produces an enterotoxin (~35 kDa) that specifically targets human claudin-4, among the 26 human claudin proteins, and causes diarrhea by fluid accumulation in the intestinal cavity. The C-terminal domain of the Clostridium perfringens enterotoxin (C-CPE, ~15 kDa) binds tightly to claudin-4, and disrupts the intestinal tight junction barriers. In this study, we determined the 3.5-Å resolution crystal structure of the cell-free synthesized human claudin-4•C-CPE complex, which is significantly different from the structure of the off-target complex of an engineered C-CPE with mouse claudin-19. The claudin-4•C-CPE complex structure demonstrated the mechanism underlying claudin assembly disruption. A comparison of the present C-CPE-bound structure of claudin-4 with the enterotoxin-free claudin-15 structure revealed sophisticated C-CPE-induced conformation changes of the extracellular segments, induced on the foundation of the rigid four-transmembrane-helix bundle structure. These conformation changes provide a mechanistic model for the disruption of the lateral assembly of claudin molecules. Furthermore, the present novel structural mechanism for selecting a specific member of the claudin family can be used as the foundation to develop novel medically important technologies to selectively regulate the tight junctions formed by claudin family members in different organs.
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Introduction
In vertebrate epithelial cell sheets that separate organs, cell-cell adhesion structures designated as tight junctions serve as almost impermeable fluid barriers. Claudins are ~23-kDa four-α-helical transmembrane proteins present in tight junctions, where they assemble into “tight-junction strands” to seal the intercellular space1,2. Mammals have 27 genes encoding claudin-family members, which are expressed tissue-specifically3. Claudin gene knockouts or mutations cause dysfunctions in the tight junction, and seriously affect tissue functions. In fact, the claudin-1 and claudin-5 knockout mice die postnatally4,5. Furthermore, deficiencies in claudin functions cause human diseases, such as neonatal sclerosing cholangitis associated with ichthyosis (claudin-1)6, deafness (claudin-11 and claudin-14)7,8, and familial hypomagnesemia with hypercalciuria and nephrocalcinosis (claudin-16 and claudin-19)9,10. The bacterium Clostridium perfringens causes food poisoning by producing the C. perfringens enterotoxin (CPE), which binds tightly to claudin-4 and less strongly to claudin-311. CPE consists of the N-terminal cytotoxic β-pore-forming domain and the C-terminal claudin-binding domain (C-CPE). The C-CPE domain disrupts the barrier and causes the endocytic internalization of tight junctions12. However, the mechanism by which the tight junction becomes disrupted has remained unknown.
Most of the claudin family members, including claudin-4, were quite difficult to prepare by cellular expression13. However, in 2014, a mutant of mouse claudin-15 was successfully prepared by cellular expression, and its crystal structure was determined13. On the basis of the crystal packing, a model of the cis interaction of claudin molecules in the tight-junction strand was proposed13. More recently, a mutant of mouse claudin-19 was prepared in a similar manner, and the crystal structure of its complex with the S313A mutant of C-CPE, C-CPE203-319S313A, which binds claudin-19 with higher affinity, was solved14. On the basis of the complex structure, a mechanism of tight-junction disruption was proposed14. However, claudin-19 is not the authentic binding target of C-CPE, and the artificial effects of the S313A mutation on the interaction with C-CPE could not be determined.
Recently, we succeeded in the preparation of human claudin-4 with a deletion of the intracellular C-terminal 26 residues (residues 1–183), by a novel cell-free protein synthesis method using an Escherichia coli cell extract15, and observed its C-CPE binding with the dissociation constant of 3.4 nM16. This success is ascribed to the use of the cell-free method, rather than cellular expression. The claudin-4•C-CPE complex was crystallized and diffracted to about 4 Å-resolution. We also prepared an N-terminal fusion of the human claudin-4 protein with T4 phage lysozyme (T4L, residues 2–162) through a glycine residue, by the cell-free method, and its co-crystal with C-CPE diffracted up to 3.5-Å resolution16.
In this study, we determined the 3.5-Å resolution crystal structure of the cell-free synthesized T4L-fused human claudin-4•C-CPE complex (designated hereafter as the human claudin-4•C-CPE complex). The crystal structure revealed the mechanism underlying the disruption of the claudin-4 assembly by C-CPE. The mode of the “on-target” interactions of C-CPE with claudin-4 is appreciably different from that of the “off-target” interactions of claudin-19 with the S313A mutant of C-CPE. This structural information will facilitate the development of novel therapeutics.
Results
The crystal structure of the complex was solved at 3.5-Å resolution (Fig. 1a, Supplementary Table S1, Supplementary Figs S1 and S2a–d). The asymmetric unit, with the space group P43, contains four non-crystallographic symmetry-related complexes (I–IV) (Supplementary Fig. S3a). These complexes essentially adopt the same overall structures (Supplementary Figs S3c and S4a–c), except for the presence of helix αX′ of C-CPE in complexes II and IV (Fig. 1b and Supplementary Figs S3a and S5a). Helix αX′ of C-CPE was not reported in the previous structure of the full-length CPE (PDB: 3AM2)17. Complexes II and IV are adjacent to each other in an upside-down manner (Supplementary Fig. S3a). In the upside-down arrangement of these complexes, the T4L tag of one complex interacts with the C-CPE of the other and probably thereby generates helix αX′, and vice versa (Supplementary Fig. S3a). The complex I structure is described hereafter, unless otherwise noted (Supplementary Fig. S3a–c).
The structure of C-CPE in complex with claudin-4 is nearly the same as that of the CPE protein itself17, indicating the absence of a conformational change in C-CPE upon complex formation (Supplementary Fig. S5a,b). The transmembrane parts of the four helices of claudin-4 superimposed well on those of mouse claudin-15 alone13 and mouse claudin-19 in complex with the S313A mutant C-CPE14 (Figs 2a–c and 3a,b). The two extracellular segments, ECS1 and ECS2, of claudin-4 both interact with C-CPE, and the complex resembles a left hand grasping the ellipsoidal C-CPE (Fig. 1c,d). The ECS1 and ECS2 regions of claudin-4 undergo conformation changes upon complex formation, as described below (Fig. 4).
The ECS1 region of claudin-4 is intimately involved in the association with C-CPE. This interaction mode of ECS1 is likely to be conserved in the S313A C-CPE complex of claudin-1914. Therefore, the hydrophobic residues in the protein-protein interface (Fig. 4a–c) were mutated (Fig. 4i). First, the F35D mutation of claudin-4 abolished the CPE-binding ability, and thus F35 is required for the association. F35 and L223′ form the closest intermolecular contact within this hydrophobic interface (Fig. 4c). Correspondingly, the L223′D mutation of C-CPE drastically impaired the claudin-4-binding ability. The effects of the F35D and L223′D mutations are much larger than those of the F35A and L223′A mutations, respectively, indicating the hydrophobic nature of the interaction of C-CPE with the ECS1 of claudin-4. In addition, ECS1 and C-CPE form extensive hydrophilic interactions neighboring the hydrophobic interface. The ECS1 β1 and β2 strands exhibit good shape complementarities to C-CPE, and interact with three hydrogen bonds, N39–R252′, N39–Y286′, and I40–Q317′ (Fig. 4d). N53 on the ‘ring finger’ (β3) of claudin-4 hydrogen bonds with S217′ (Fig. 4e). Actually, the N53A and N53D mutations decreased the CPE-binding ability of claudin-4 (Fig. 4j). In this context, the deletion of residues 184′–220′ of C-CPE reportedly decreased its affinity for claudin-412.
The claudin-4 ECS2 (the thumb in Fig. 1d) is also involved in the association with C-CPE. In contrast to the ECS1 interactions, this interaction mode is not conserved in the claudin-19 complex14. First of all, L151 from the claudin-4 ECS2 is accommodated in the “triple tyrosine pit”, consisting of Y306′, Y310′, and Y312′, in the present complex structure (Fig. 4f), which is consistent with the previous mutagenesis studies18. In addition, D146 from the claudin-4 ECS2 domain interacts with R227′ of C-CPE (Fig. 4b,g). A previous mutagenesis analysis of D146 revealed the importance of this residue in CPE binding19, and the present study has now identified its interaction partner as R227′. Furthermore, the C-CPE-binding interface of the claudin-4 ECS2 domain involves the hydrogen bond S154–D284′ (Fig. 4h). Notably, CPE binds claudin-4 much more strongly than the other claudins. Among the claudins, the ECS2 domain of claudin-4 exhibits much lower sequence homology than the ECS1 domain (Supplementary Fig. S6, V2 region), and has been discussed as the site that defines the CPE-binding specificity20.
We compared our crystal structure of the human claudin-4•C-CPE complex with that of the mouse claudin-19•C-CPE203-319S313A complex (PDB: 3 X 29)14 (Fig. 3a,b). Although claudin-19 is not a biological target of CPE, it still binds to C-CPE with about 70-fold weaker affinity than claudin-414. We detected several important differences between the two structures. First, the orientation of C-CPE relative to the transmembrane helices is different, by ~10°, between the claudin-4 and claudin-19 complexes (Fig. 3b). The extracellular part of the third TM helix of claudin-4 is bent by ~10° toward the outside, as compared to that of claudin-19, while in contrast, the transmembrane parts of the third TM helices are highly superimposable (Fig. 3b).
Second, twelve interactions between claudin-4 and C-CPE were observed (Fig. 4c–h), but five of them, N39–Y286′, Q44–N218′, Q44–N222′, N53–S217′, and L151–Y306′/Y310′/Y312′ (triple tyrosine pit), are missing in the claudin-19•C-CPE203-319S313A complex14. Conversely, six C-CPE203-319S313A-interacting residues of claudin-19 (D38–K283′, S53–N218′, T153–R227′, T153–S256′, N156–D284′, and Y159–D225′)14 are missing among the C-CPE-interacting residues of claudin-4.
The F35 residue in claudin-4 is replaced by Tyr in claudin-19. In addition, L151 in claudin-4, which interacts with the triple tyrosine pit, is replaced by Ser in claudin-19 (Fig. 3a). Therefore, the hydrophobic interaction of C-CPE with claudin-19 is much weaker than that with claudin-4. Furthermore, S38 in the ECS1 of claudin-4 is replaced by Asp in the ECS1 of claudin-19 (Fig. 3a), in which D38 forms a salt bridge with K283′. According to Saitoh et al.14, the D38A mutation of claudin-19 strengthens the interaction with C-CPE14. Therefore, the salt bridging D38–K283′ interaction prevents the claudin-19•C-CPE complex from forming the correct conformation around the ECS1 of claudin-19.
Why does C-CPE203-319S313A tightly bind to claudin-19? The structures of C-CPE and C-CPE203-319S313A are very similar to each other (Fig. 5a,b). In the present structure, the side chain of R227′, near S313′, of C-CPE and that of D146 of claudin-4 face each other (Fig. 4g). However, the side chain of E147 in claudin-19 is longer than that of D146 in claudin-4, by about 1.5 Å. Therefore, when the wild-type C-CPE binds to claudin-19, the E147 side chain is as close as 1.3 Å to the R227′ side chain of C-CPE (Fig. 6). Actually, in the claudin-19•C-CPE203-319S313A complex14, the side chain of R227′ of C-CPE203-319S313A is bent. The mutation of S313′ to alanine provides sufficient space for R227′ to avoid the steric hindrance with E147. Consequently, C-CPE203-319S313A can bind tightly to claudin-19.
We superimposed a homology model of the human claudin-4 apo form, constructed on the basis of the structure of the mouse claudin-15 apo form13, on the present claudin-4•C-CPE complex structure (Fig. 2b,c and Fig. 7a,b). The superimposition revealed that significant conformation changes occur upon CPE binding, in both the ECS1 and ECS2 domains. In the apo form, a short α helix, designated as the extracellular helix (ECH), exists between β4 and α2, whereas this region is unwound and extended in the C-CPE-bound form (Figs 2b and 7a). In the ECS2 region, the loop between the extracellular part of helix α3 and strand β5, designated as the V2 region, assumes different conformations between the two forms (Fig. 7b). In contrast, the other parts of the ECS1 region (β1 and β2) and the transmembrane helix bundle are highly superimposable (Fig. 2b). Consequently, the C-CPE can bind firmly to this rigid foundation (the transmembrane helices and the ECS1 β1 and β2) of claudin-4. The α3′ helix of C-CPE then pushes strand β4 outward by about 2 Å toward the ‘outside of the hand’ (Figs 1b and 7a). Concomitantly, the van der Waals interaction is disrupted between Y67 and L71 on the ECH and L77 on helix α2. Moreover, the region between β4 and α2 is significantly expanded, as strand β4 shifts with no movement of the transmembrane α2 helix. These conformation changes in this region must unwind the ECH (Fig. 7c). Concomitantly, the extracellular part of helix α3 is wound up by about 20°, and thus D146 rotates, together with F147 and Y148, and forms a salt-bridge with R227′ of C-CPE (Fig. 7b). At the same time, L151 shifts by about 4 Å, and is inserted into the triple tyrosine pit of C-CPE. These conformation changes, coupled with the association between ECS2 and C-CPE, result in the movement of the V2 region, which shifts by as much as 7 Å to avoid steric hindrance with D225′ and S313′ of C-CPE (Fig. 7b).
What is the mechanism of tight-junction disruption triggered by C-CPE binding? It was proposed that the crystal packing of mouse claudin-15 along the b axis reflects the cis assembly of claudin-15 molecules with the tight-junction strand on the cell surface. This putative cis assembly is mediated by the interactions between the ECH of one molecule and the ECS2 of the next, or the intermolecular hydrophobic interactions between M68 (L70 in claudin-4) of the ECH region and F146 (F147) of the ECS2 region (box I in Fig. 8a)13. Our homology model of the apo-form and the crystal structure of the C-CPE-bound form of claudin-4 along the b axis of the mouse claudin-15 crystal are shown in Fig. 8a. The model indicated that the cis assembly of claudin-4 can be formed in the same manner as claudin-15. Together, these observations suggested that the conformation changes in the region around the ECH and V2 regions that occur upon CPE binding (Fig. 7a–c) disrupt the putative cis assembly of claudin-4 (box I, Fig. 8a).
Furthermore, if two C-CPE molecules bind to a pair of neighboring claudin-4 molecules arrayed in cis, then they would cause unavoidable steric hindrance (Fig. 8a, box II). On the basis of the cis assembly of claudin-4 (Fig. 8a), we propose a model of the tight-junction pore structure, including the possible trans assembly with C-CPE molecules on the opposite cell, as shown in Fig. 4b. In the case of claudin-2, a cysteine residue substituting for D65 (K65 on the palm side of the little finger of claudin-4) could form a disulfide bond within the trans assembly21. Therefore, it is highly likely that the 65th position of one claudin-4 molecule is close to another claudin-4 molecule in the trans interaction (Fig. 8b). Although a pore (6.5–8 Å, ref. 22) exists between the palms of the trans-interacting claudin molecules, the present binding mode of C-CPE to the palm side of claudin-4 is not compatible with tight-junction formation, considering the size of C-CPE (approximately 40 × 25 × 25 Å3) and the full-length CPE (95 × 42 × 32 Å3)17 (Supplementary Fig. S7). This spatial competition for the claudin palm between the trans claudin molecule and the CPE might be one of the inhibition mechanisms (Fig. 8c).
The present novel findings on the mechanisms of tight-junction disruption might facilitate the development of anti-enterotoxin therapeutics. The previously reported C-CPE mutants that target other claudins were designed without any knowledge of the claudin structures23,24. This study now provides information about the claudin-4•C-CPE structure with the C-CPE-induced conformation changes of claudin-4. Therefore, the present complex structure is strongly expected to enable the rational design of novel C-CPE mutants that target other claudins, and innovative medically important technologies to regulate selectively, at will, the tight junctions formed by claudin family members in different organs.
Methods
Structure determination
The details of the cell-free synthesis of T4L-claudin-4 and C-CPE, the purification and crystallization of the T4L-claudin-4•C-CPE complex, and the X-ray diffraction data collection, data processing, and initial phasing have been described (Shinoda et al.16). The electron density maps corresponding to the α helices and the β strands of human claudin-4 were visualized by the molecular replacement (MR) – single-wavelength anomalous dispersion (SAD) method, using the program Phaser-EP25 in the PHENIX suite26, with the MR solution including four C-CPE molecules and two T4 lysozyme molecules. The Cα atoms of the α helices and the β strands of claudin-4 were traced, using the program Find Helices and Strands (PHENIX) with the density modified map. Using the MR-SAD method with the P43212 crystal dataset which was collected using BL32XU at SPring-827,28 and the preliminary model described above, the locations of 24 of the 30 selenium atoms were determined (Supplementary Fig. S8). On the basis of the positions of the identified selenium atoms, those of the other residues of claudin-4 were assigned. For the parts in which the residues could not be placed automatically, the amino acid residues were manually placed with the program COOT29. The atomic model was refined, using the restraints from the experimental phases, by repetitive model building and map calculations with the programs COOT and phenix.refine (PHENIX). NCS restraints were used in the refinement with the low resolution data, but not in the final refinement with the high resolution data. The quality of the model was examined with MolProbity30. The Ramachandran statistics of this model were 92.65% (favored), 7.30% (allowed), and 0.06% (outliers), and the clash score was 7.78. The Rwork/Rfree values were 0.29/0.31. Structural graphics were drawn using PyMol (www.pymol.org) and UCSF Chimera31. The electrostatic potential on the surface of our structural model was calculated using the Adaptive Poisson-Boltzmann Solver32.
Homology modeling
Homology modeling of the apo form of human claudin-4 was performed separately for the N-terminal part (M1–T34) and the C-terminal part (N42–S186) by SWISS-MODEL33, with the crystal structure model of mouse claudin-15 (ref. 13, PDB: 4P79) as the template. Helix α4 was not well modeled by SWISS-MODEL in the homology model of the claudin-4 apo form, possibly because its formation depends on the lipid bilayer environment. Therefore, the structure of α4 in mouse claudin-15 was retained, with manual replacements in the human claudin-4 sequence with COOT. The coordinate sets of the N- and C-terminal parts were merged, and refined by the program in CNS34 with no experimental energy term.
Pull-down assay
The human claudin-4 (full-length) and the glutathione-S-transferase-tagged C-CPE (residues 185′–319′) (GST-C-CPE) used in this assay were both prepared by the E. coli cell-free protein synthesis method (Supplementary Fig. S9a–d). Approximately 10 μg of claudin-4 were incubated with 10 μg of GST-C-CPE at 4 °C for 1 hr with shaking, and then the solution was mixed with 20 μl of glutathione Sepharose resin (GE) at 4 °C for 2 hr with shaking. The resin was washed with 40 column volumes of 50 mM Tris-HCl buffer (pH 7.0), containing 0.05% β-dodecyl-D-maltopyranoside (βDDM, Anatrace), 0.002% cholesterylhemisuccinate (CHS, Anatrace), and 400 mM NaCl, and eluted with 50 μl of 50 mM Tris-HCl buffer (pH 8.0), containing 20 mM reduced glutathione, 0.05% βDDM, 0.002% CHS, and 400 mM NaCl. The eluates were analyzed by SDS-PAGE and CBB-staining (Supplementary Fig. S10). Densitometry of the bands in the SDS-PAGE gel was performed using ImageJ (http://imagej.nih.gov/ij/). The normalized bound values were calculated from the ratio of the density of the claudin band to the density of the corresponding C-CPE band, and normalized to the bound amount of the control sample (claudin-4 wild type and C-CPE wild type). Statistical analysis was performed using Welch’s t-test in Microsoft EXCEL.
Additional Information
Accession codes: The Swiss-Prot accession codes for human claudin-4 and CPE are O14493 and Q0SVZ0, respectively. The coordinates and structure factors for the structure of the human claudin-4•C-CPE complex have been deposited in the Protein Data Bank, under the accession code 5B2G.
How to cite this article: Shinoda, T. et al. Structural basis for disruption of claudin assembly in tight junctions by an enterotoxin. Sci. Rep. 6, 33632; doi: 10.1038/srep33632 (2016).
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Acknowledgements
We thank K. Hasegawa and H. Okumura (the Japan Synchrotron Radiation Research Institute (JASRI), BL41XU of SPring-8) for X-ray data collection, Professor M. Furuse (Kobe University) and Professor Y. Horiguchi (Osaka University) for the kind gift of the gene encoding C-CPE, M. Aoki, M. Inoue, M. Goto, T. Yamaguchi, and K. Ishii for plasmid preparation, R. Akasaka for sample assessment, and Y. Nakamura for assistance in X-ray data analysis. The synchrotron radiation experiments were performed at the BL32XU and BL41XU of SPring-8 with the approval of RIKEN and JASRI (2014A1502, 2013B1502, 2013A1401, and 2012A1329). This work was supported by the Targeted Proteins Research Program (TPRP) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (S.Y.); the Platform Project for Supporting in Drug Discovery and Life Science Research (Platform for Drug Discovery, Informatics, and Structural Life Science) from MEXT and Japan Agency for Medical Research and development (AMED) (S.Y.); and a Grant-in-Aid for Young Scientists (B) (25840028) from MEXT (T.S.).
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T.S., T.K.-S., S.Y. and M.S. designed the research. N.O. prepared the claudin and C-CPE expression plasmid. T.S., N.S. and K.I. performed protein expression and purification. T.S. and T.T. assayed purified protein samples. T.S. performed crystallization, X-ray data collection and analysis, and structure determination. K.H., Y.K. and M.Y. assisted with X-ray data collection. T.S., T.K.-S., S.Y. and M.S. wrote the manuscript.
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Shinoda, T., Shinya, N., Ito, K. et al. Structural basis for disruption of claudin assembly in tight junctions by an enterotoxin. Sci Rep 6, 33632 (2016). https://doi.org/10.1038/srep33632
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DOI: https://doi.org/10.1038/srep33632
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An update on the human and animal enteric pathogen Clostridium perfringens
Emerging Microbes & Infections (2018)
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