Abstract
Hematopoietic stem cells (HSC) demonstrate natural variation in number and function. The genetic factors responsible for the variations (or quantitative traits) are largely unknown. We previously identified a gene whose differential expression underlies the natural variation of HSC numbers in C57BL/6 (B6) and DBA/2 (D2) mice. We now report the finding of another gene, Slit2, on chromosome 5 that also accounts for variation in HSC number. In reciprocal chromosome 5 congenic mice, introgressed D2 alleles increased HSC numbers, whereas B6 alleles had the opposite effect. Using gene array and quantitative polymerase chain reaction, we identified Slit2 as a quantitative trait gene whose expression was positively correlated with the number of HSCs. Ectopic expression of Slit2 not only increased the number of the long-term colony forming HSCs, but also enhanced their repopulation capacity upon transplantation. Therefore, Slit2 is a novel quantitative trait gene and a positive regulator of the number and function of murine HSCs. This finding suggests that Slit2 may be a potential therapeutic target for the effective in vitro and in vivo expansion of HSCs without compromising normal hematopoiesis.
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Introduction
Stem cells are key to homeostatic maintenance of mature, functional cells in a variety of tissues and organs. They self-renew and produce progeny to replenish dying or damaged cells throughout an organism’s lifetime. Thus, functional failure of tissue-specific stem cells may limit tissue repair and renewal, deteriorate organismal health and contribute to disease development1,2. The stem cells responsible for production of all blood cells are hematopoietic stem cells (HSCs), a rare cell population located in adult bone marrow. Because of the unprecedented experimental model systems that are available for exploration of HSCs, stem cell research in the field of hematology has been the subject of extensive studies3. It is likely that the same broad concepts defining blood-forming stem cells will apply to stem cell populations in other tissues and organs.
Stem cell regulation is a complicated and dynamic process. Identification of the collection of genes contributing to critical stem cell functions, self-renewal and multi-lineage differentiation, is far from complete. Thus, complementary strategies are needed to unravel this complex regulatory network4. The most widely used approach for functional analysis of specific genes is based on artificial manipulations through knockdown, overexpression or mutation in animal models. Alternatively, natural diversity and complexity of cellular traits can be linked to particular genetic variations, thus providing a powerful yet underutilized tool for the discovery of gene function5,6,7,8. This approach, proceeding from phenotype to genotype, successfully revealed genes involved in the regulation of a variety of complex traits including obesity, blood pressure, arthritis and fatty acid metabolism9,10. Very few such genes, however, have been found in stem cells. In this study, we identified a stem cell regulatory gene accounting for the natural variation in HSC number in two mouse strains, C57/BL6 (B6) and DBA/2 (D2).
B6 and D2 mice, two commonly used inbred strains, are beneficial models for genetic mapping of phenotypic variations. We previously revealed variations in a number of HSC traits between these strains in which B6 mice have fewer HSC numbers whereas D2 mice have more. We further identified responsible quantitative trait loci (QTL) with genome-wide scans of linked genetic makers11,12,13,14,15,16,17,18,19. Using congenic mouse strains in which the QTL region is exchanged between two parental strains and subsequent oligonucleotide arrays, we successfully discovered the first quantitative trait gene (QTG), latexin (Lxn) and its regulatory role in HSC number and function20. Lxn expression is negatively correlated with the natural variation of HSC numbers: high Lxn level is associated with low HSC numbers in B6 mouse, whereas low Lxn expression is linked to high stem cell numbers in D2 mice. Lxn regulates the HSC population via a concerted mechanism of increasing stem cell self-renewal proliferation and decreasing apoptosis20. In an extension of this phenotypic genomic approach, several studies employed a panel of genes differentially expressed between B6 and D2 cells as a trait to map QTL that modulate gene expression (i.e., expression QTL, or eQTL)21. Distinct groups of eQTL, acting as either cis- or trans- controlling elements, were identified to define gene expression profiles that are specific to a single cell type and its functions, or to cellular differentiation state in a group of developmentally related cells22,23,24.
In this study, we employed the classic phenotypic genomic approach and we report the finding of an additional, novel QTG, Slit2, which also modifies HSC number variation in B6 and D2 strains via a unique mechanism unlike that of Lxn. Slit2 expression is positively correlated with HSC numbers and is affected by the genetic background. Increased expression of Slit2 led to a three-fold in vitro and in vivo expansion of the HSC compartment and conferred a competitive advantage to HSCs upon transplantation. These findings may prove to be of clinical value in strategies for the effective expansion of HSCs in bone marrow transplantation for treatment of hematologic malignancies.
Results
Generation of chromosome 5 congenic strains
We previously identified three QTLs that contribute to natural variation in HSC number between B6 and D2 mice. We produced congenic mouse strains for each QTL and successfully discovered a QTG (Lxn) on chromosome 3 (Chr3) and confirmed its function in HSC regulation13,20. In the current study, we focus on a QTL on chromosome 5 (Chr5; Fig. 1A) and aim to unravel novel HSC regulatory mechanisms. Mouse strains congenic for the Chr5 QTL were generated by crossing the genomic interval harboring the QTL using B6 as the donor strain and D2 as the recipient strain (symbolized as D.B Chr5) and vice versa (B.D Chr5). The two reciprocal congenic strains were derived and genotyped as described previously25. Both congenic lines were homozygous within their respective congenic intervals. The congenic interval in Chr5 that is mutually inclusive in reciprocal strains spans from 20.8 Megabases (Mb) (9.8 centiMorgan (cM ± 1cM)) to 53 Mb (28.5cM ± 1cM) and the location of the marker (D5Mit352) most tightly linked to the trait is in 35.9 Mb (18.4cM ± 1cM) (Fig. 1B).
Confirmation of the functional effect of Chr5 QTL in congenic strains
To validate QTL linkage analysis, we performed a long-term in vitro culture assay, the cobblestone area-forming cell (CAFC) assay, on bone marrow cells derived from Chr5 congenic mice and determined cobblestone areas at day 35 of culture, which is a proxy for the number of long-term HSCs that were present in the seeded bone marrow cells. Introgression of D2 alleles onto a B6 genetic background in the B.D Chr5 congenic line led to a three-fold increase in HSC number as compared to B6 background (Fig. 2A), whereas transfer of B6 alleles onto the D2 genetic background decreased HSC number by 50% in the D.B Chr5 strain. Moreover, cell counts for peripheral blood leukocytes, erythrocytes and platelets and numbers for CAFC days 7 and 21, which represent hematopoietic progenitor cells at different stages, showed no difference between Chr5 congenic and their respective parental strains, except for CAFC day 21 between D2 and D.B Chr5 mice (Suppl. Table 1), suggesting that the non-consensus congenic region in D.B Chr5 (53 –65 Mb, see Fig. 1B) mouse may affect hematopoietic progenitor cell (HPC) number. To further confirm the CAFC result, we quantified the immunophenotypic HSCs, which are defined as cells negative to lineage markers (Lin-), positive for Sca-1 and c-kit (Sca-1+ and c-Kit+) and negative for CD34 (CD34-) (Fig. 2B). Consistently, the result showed that B.D Chr5 mice had more HSC/HPC-enriched Lin-Sca-1 + c-Kit+ (LSK) cells (Fig. 2C) and HSC-enriched LSKCD34- cells (Fig. 2D) than B6 mice, whereas reciprocal D.B Chr5 mice had fewer cells in these two populations compared to D2 mice. Altogether, these results suggest that Chr5 QTL specifically regulates the size of the HSC population and that the D2 allele is associated with an increased stem cell number whereas the B6 allele is associated with decreased stem cell number.
Chr5 QTL enhances HSC repopulation capacity in a cell-intrinsic manner
To determine whether Chr5 QTL can intrinsically regulate HSC number and function, we performed the competitive repopulation assay, in which B6 or B.D Chr5 bone marrow cells were transplanted into the same recipients (or environment) and repopulated their blood system (Fig. 3A). B.D Chr5-derived cells made a greater contribution to reconstitution of peripheral blood leukocytes, especially T lymphocytes, total bone marrow cells and HSC/HPC-enriched LSK cells (Fig. 3B–D), suggesting that D2 QTL enhances HSC repopulation capacity. Moreover, The increased level of LSK cells in B.D Chr5 cells reconstituted recipients was similar to that in B.D Chr5 congenic mice (Fig. 2C), indicating the cell-intrinsic role of this congenic interval. It should be noted that the recipient mice carrying CD45.1 markers are not commercially available on D2 genetic background, which precluded us from performing competitive repopulation assay on D2 and D.B Chr5 bone marrow cells. We further determined the cell cycle and apoptotic status of congenic and background HSCs because both cellular mechanisms contribute to maintenance of the HSC pool. No differences in percentages of cycling and apoptotic cells were detected in congenic strains compared to their respective background strains (B.DChr5 vs B6 and D.BChr5 vs D2) (Supp. Fig. 1A,B). However, a significantly higher percentage of LSKCD34- HSCs underwent apoptosis in mice with B6 background than those with D2 background (Supp. Fig. 1B), suggesting the effect of genetic background on the function of HSCs. Altogether, these results imply that Chr5 QTL and underlying QTGs are involved in the regulation of repopulation and multi-lineage differentiation of HSCs in a cell-intrinsic manner.
Identification of candidate QTGs by microarray and quantitative polymerase chain reaction (PCR)
In order to investigate QTG underlying Chr5 QTL, we obtained gene expression profiles on HPC/HSC-enriched LSK cells of B6, B.DChr5, D2 and D.BChr5 strains using the Illumina microarray platform. We revealed 1,791 differentially expressed genes between B6 and B.DChr5 HSCs and 470 differentially expressed genes between D2 and D.BChr5 cells (GEO Accession # GSE21896), in which 153 genes overlapped between the two comparison groups (Fig. 4A). We screened these genes on the basis of genomic location within the 95% confidence interval (20.8–53 Mb) surrounding the Chr5 QTL and identified three candidate genes: Slit2, QDPR and Gpr125. Quantitative real-time PCR confirmed that only Slit2 showed reproducible patterns of differential expression in LSK cells in both congenic-parental strain comparisons (Fig. 4B). The presence of D2 alleles in the congenic interval resulted in a high level of Slit2 expression relative to almost undetectable levels in mice bearing the B6 genotype. A roughly 30-fold increase in Slit2 expression was observed in B.D Chr5 LSK cells relative to B6 and a similar fold decrease in D.BChr5 LSK cells relative to D2. These data suggest that Slit2 expression is positively correlated with HSC numbers and its expression pattern in HSCs is dramatically affected by genetic background.
eQTL is associated with the differential expression of Slit2 between B6 and D2 mice
Differential expression of Slit2 between both congenic and parental strains suggests that Slit2 expression is governed by a polymorphic regulatory region within the congenic interval. Consistent with this observation, 3 consecutive, highly significant eQTL were identified for the Slit2 transcript in HSCs using the “genetical genomics” approach (http://genenetwork.org/webqtl/main.py). The peak eQTL (Likelihood Ratio Statistic = 37.952) corresponds to 13 consecutive markers spanning 45.6–53.1 Mb on Chr5 (Fig. 4C) and encompasses the entire Slit2 transcript (48.376032–48.696750 Mb). In addition, the overlapping green line in the eQTL peak suggests that the D2 allele is associated with high Slit2 expression, which corroborates our microarray and real-time PCR results. Our discovery of Slit2 differential expression in B6 and D2 LSK cells and the search for responsible eQTL are consistent with results by Gerrits et al., which also identified distinct specificity of Slit2 eQTL in a stem cell population23. These results suggest that the Slit2 gene is cis-regulated and the single nucleotide polymorphisms flanking the Slit2 gene most likely contribute to its differential expression in B6 and D2 HSCs.
Ectopic Slit2 expression results in expansion of functional HSCs
Having established a positive correlation between Slit2 expression and HSC numbers under physiologic conditions, we enforced Slit2 expression in HSC/HPCs and determined its direct regulatory role in HSC number and function. Using the same overexpression strategy as previously published20, we cloned the Slit2 cDNA into a retroviral vector containing the green fluorescent protein (GFP) reporter and overexpressed Slit2 in HSC/HPCs from B6 mice, which normally express little or no Slit2. HSC number was measured by CAFC assay on GFP positive bone marrow cells (GFP control or Slit2-GFP) (Fig. 5A). The increased expression of Slit2 at mRNA and protein levels was confirmed by real-time PCR and Western blot, respectively, in Slit2-GFP cells compared to control GFP cells (Fig. 5B,C). Overexpression of Slit2 resulted in a 3-fold increase in the number of HSCs (CAFC day 35) (Fig. 5D), strongly suggesting Slit2 as a positive regulator of HSC number. In order to determine in vivo function of Slit2 in hematopoiesis, we transplanted 2 × 105 Slit2-GFP or control GFP cells along with the same number of competitor cells (a standard approach in murine bone marrow transplantation studies to ensure recipient survival) into lethally irradiated recipient mice and measured reconstitution of GFP+ cell in peripheral blood at different time-points post-transplant. Slit2-overexpressing cells (Slit2-GFP) made a greater contribution to blood reconstitution at both short-term (4 weeks and 8 weeks) and long-term (16 weeks) post-transplant (Fig. 5E), demonstrating that Slit2 can increase the repopulating capacity of HSCs. This result is consistent with that seen in B.D Chr5 congenic HSCs, which have naturally high levels of Slit2 expression (Fig. 3B). To further confirm the positive regulatory role of Slit2 in HSC numbers, we performed a more stringent assay (i.e., long-term limiting-dilution analysis in competitively repopulated hosts) to functionally identify and quantify HSCs in vivo. We found that overexpression of Slit2 caused a 3-fold increase in the number of long-term repopulating HSCs (Fig. 5F). Thus these results robustly suggest Slit2 as a positive regulator of HSC number and function.
Discussion
In this study, we identified a new quantitative trait gene, Slit2, as the positive regulator of HSC number and function. We used the QTL mapping method, which is a powerful approach for discovering genetic determinants of complex traits8. We began with the observation of natural HSC number variation between two genetically distinct mouse strains (Fig. 6): the B6 strain has lower stem cell numbers and the D2 strain has higher numbers. Genetic mapping analysis identified a locus on chromosome 5 that is associated with this natural variation. We then generated reciprocal congenic mouse strains in which the genomic locus harboring the QTL was exchanged between the B6 and D2 strains. Quantification of HSC number in these congenic strains confirms that the chromosome 5 QTL and the candidate gene(s) must not only be located within the congenic interval but, if functioning at the level of the transcript, the regulatory element governing expression must also be present in the congenic interval. The congenic mice were also particularly valuable in microarray analyses to identify candidate genes. Among the 2,261 unique differentially expressed transcripts identified in the Illumina microarray experiment, only 153 were common between the two congenic/background strain comparisons, representing a 94% reduction in the number of genes differentially expressed between the B6 and D2 parental strains alone. This reduction greatly facilitated our selection of Slit2 as a candidate gene because it is the only differentially expressed gene that is present in the consensus congenic interval. Therefore, given the novel role for Slit2 in the hematopoietic system, we benefited greatly from the ability of the QTL mapping approach to eliminate bias introduced by selecting genes on the basis of a priori knowledge about function. Elimination of Slit2 in knock-out mice results in embryonic lethality26. Unlike mutagenesis techniques, lethality or nonspecific effects are not hampered by QTL-based gene discovery, thus preventing detection of a phenotypic effect on adult tissue.
The mechanisms by which Slit2 regulate hematopoiesis have not been identified. However, several recent publications have reported the function of its binding partner, Robo4, in HSC regulation27,28. Smith-Berdan and colleagues unraveled the important role of Robo4 in anchoring HSCs to bone marrow niche. Loss of Robo4 specifically decreased the number of HSC in bone marrow and impaired bone marrow engraftment capability. Interestingly, absence of Robo4 was compensated with up-regulation of CXCR4, which led to less efficient mobilization of HSCs from bone marrow to the periphery. The authors also investigated the role of Slit2 in Robo4 function and found that Slit2 didn’t affect HSC proliferation and migration in vitro. The study by Shibata et al. showed that Slit2 was specifically expressed in bone marrow stromal cells of the B6 strain, but not HSCs and overexpression of Slit2 led to the differentiation of primitive HSCs to less-primitive marrow cells. In addition to HSCs, a recent study has shown that Slit2 transgenic mice have increased intestinal stem cell numbers29. Our findings are in agreement, at least partly, with these literature reports. We found that Slit2 and the Slit2-containing Chr5 QTL do not influence HSC proliferation and apoptosis (Supp. Fig. 1). Like intestinal stem cells, overexpression of Slit2 led to the increased number of functional clonogenic and repopulating HSCs (Fig. 5). More importantly, our study demonstrates for the first time the strain-specific difference in Slit2 expression in physiological conditions. Indeed, the function of Slit2 in HSCs seems to be overlooked due to the fact that very little or no detectable Slit2 transcript was present in HSCs of the B6 mouse strain compared to the D2 strain (Fig. 4B). This observation not only explains the reason that Slit2 was not detected in HSCs in Shibata’s study, but also reveals the importance of genetic background in modification of critical stem cell regulatory genes. It would be intriguing to employ the D2 mouse strain as a model to investigate Slit2 function in the regulation of HSCs and stromal cells in future studies.
The role of Slit2 as a secreted protein in governing cellular migration, spatial orientation, organogenesis and development in other tissues is well known30,31,32,33,34. Here, we provide strong evidence to support the cell-intrinsic role of Slit2 in increasing HSC numbers. In competitive repopulation assays in which B6 or B.D Chr5 cells were injected into the same environment (recipients), B.D Chr5 showed an advantage in repopulating blood and bone marrow stem/progenitor cells (Fig. 3). Moreover, Slit2 overexpressing cells showed a similar increase in their repopulating capacity and in the absolute number of repopulating cells upon transplantation (Fig. 5E,F). Such changes are similar to what we observed in the B.D Chr5 congenic mice in situ, strongly supporting that Slit2 plays an intrinsic role in regulating HSC function. However, because Slit2 is also highly expressed in the bone marrow niche cells, such as osteoblast and mesenchymal stem cells35, we cannot exclude the possibility that Slit2 regulates HSC numbers by influencing stem cell-niche interaction. It is possible that high Slit2 expression may result in preferential localization in niches that favor stem cell self-renewal and low Slit2 expression results in occupation of niches that favor differentiation36,37,38. In addition, compensatory alterations in other signaling pathways, such as CXCR4, may account for important differences in HSC number and functions between B6 and D2 mice. Further experiments, such as reciprocal transplantation with B6 or B.D Chr5 mice as recipients, will be interesting to determine the cell-intrinsic and/or cell-extrinsic role of Slit2 in regulating HSC function.
Our previous studies identified Lxn on the Chr3 QTL as a negative regulator of HSC number and Chr3 and Chr5 QTLs as contributing loci for the natural variation of HSC number in B6 and D2 mouse strains20. In combination with discovery of Slit2 as a positive regulator herein, it will be of great interest to investigate synergetic and/or additive roles of these genes in homeostatic maintenance of the HSC population size. It may unravel a novel signaling pathway for HSC regulation. Although no evidence functionally links Lxn and Slit2, a recent study showed that Lxn is co-localized with Robo4 in hematopoietic stem/progenitor cells and its ablation reduces the abundance of Robo4 protein39. We speculate that loss of Lxn expands the HSC population and HSCs reflexively down-regulate Robo4 expression and decease HSC numbers. Thus, Lxn and Slit2/Robo4 signaling pathways may counteract each other and cooperatively control HSC population size in normal physiological conditions. We are currently investigating this hypothesis. If it can be experimentally supported, it highly underscores the importance and power of the QTL approach in uncovering a genetic regulatory network in stem cell function. Uncovering the genetic networks that govern natural variation in complex traits such as stem cell numbers make it possible to identify a specific group of regulatory genes as well as their cooperative roles in determining stem cell functions. It would be difficult to appreciate the complexity of such networks, governing a homeostatic mechanism like the maintenance of hematopoiesis, without the QTL to QTG strategy.
In summary, we report compelling evidence for the identification of Slit2 as a QTG on mouse Chr5 that influences HSC numbers in mice. Gene expression analysis of primary hematopoietic cells, overexpression and functional studies reveal a novel role for Slit2 as a positive regulator of HSC numbers. It is likely that polymorphisms between B6 and D2 in the Slit2 or a nearby cis regulatory element are responsible for strain-dependent differential expression. These findings may lead to effective strategies for in vitro and in vivo expansion of the HSC population, enhancement of HSC mobilization and engraftment and improved strategies for HSC transplantation therapy.
Methods
Animals
Six to 16 week old female C57BL/6J(CD45.2), B6.SJL/J(CD45.1) and DBA/2J mice were purchased from The Jackson Laboratory (Bar Harbor, ME) and congenic mice generated by our laboratory were used in all experiments. Mice were given food and acidified water ad libitum and housed in pathogen free conditions in the animal facility of the University of Kentucky and were maintained according to NIH guidelines for animal welfare. All animal and experimental procedures were approved by IACUC and IBC offices of the University of Kentucky with protocol numbers 2012-0999 (IACUC) and B13-2133 (IBC).
Generation of Chr5 congenic strains
Congenic strains were generated by selective, marker-assisted breeding as previously described25. The genomic interval carrying the QTL from B6 was transferred onto a D2 genetic background to generate the D.B Chr5 congenic strain. The reciprocal congenic, designated B.DChr5 was generated by transfer of the D2 QTL region onto the B6 background. Both strains were bred to homozygosity at all loci, as verified by genotype analysis with 100 microsatellite markers distributed across the mouse genome. The consensus congenic interval ranges from 20.8–53 mega bases on Chr5, encompassing the entire 95% confidence interval identified by linkage analysis.
Peripheral blood cell counts
Anesthetized mice were bled from the retro-orbital venous plexus. Circulating leukocyte, erythrocyte and platelet counts were measured by analysis of 40 ul blood using a System 9118+ Hematology Series Cell Counter (Biochem Immunosystems, Allentown, PA).
Cobblestone area-forming cell assay
CAFC assay was performed as previously described to quantify hematopoietic stem and progenitor cells20. We performed simultaneous analysis of CAFCs in B6, D2 and congenic strains as well as in Slit2-GFP vs GFP control cells. In brief, a confluent monolayer of FBMD-1 stromal cells was established in 96-well tissue culture-treated plates (Costar, Cambridge, MA). After 7 to 10 days, wells were seeded either with unfractionated marrow or GFP+ cells at a dose of 81,000, 27,000, 9,000, 3,000, 1,000, or 333 cells per well. Twenty replicate wells per cell number were evaluated. The cells were cultured in Iscove’s Modified Dulbecco Medium, containing 20% horse serum, 80 U/mL penicillin, 80 mg/mL streptomycin (all from Life Technologies), 10−4 ß-mecaptoethanol and 10−5 M hydrocortisone (both from Sigma, St. Louis, MO). Individual wells were screened at days 7, 14, 21, 28 and 35 for the presence of a cobblestone area, defined as a colony of at least 5 small, non-refractile cells growing underneath the stroma. The longer the latency before cobblestones appear, the more primitive the cells forming that cobblestone. Thus, the most primitive HSCs show cobblestones at day 35, whereas colonies that appear earlier are derived from more committed progenitor cells. Frequencies of CAFCs were calculated by using maximum likelihood analysis and equals 1 divided by the number of cells yielding 37% negative wells. Statistical comparisons were performed using the L-Calc software package from Stem Cell Technologies (Vancouver, Canada). Statistically significant difference between populations being compared was determined using a p-value threshold of 0.05.
Immunofluorescent staining and sorting of hematopoietic stem cells
Bone marrow cells suspended in Hank’s balanced salt solution (HBSS, Gibco, Grand Island, NY) containing 2% fetal calf serum (FCS) (Life Technologies) were blocked with anti-CD16/32 (clone 2.4G2, Fc Block) to prevent non-specific binding. Cells were stained with the lineage markers, including CD5 (clone 53-7.3), CD8a (clone 53-6.7), CD45R/B220 (clone RA3-6B2), CD11b/Mac-1 (clone M1/70), Ly-6G/Gr-1 (clone RB6-8C5), TER119/Ly-76 (clone TER-119) and stem cell-specific markers, Ly-6A/E (Sca-1; clone E13-161.7), CD117 (c-kit; clone2B8) and CD34 (clone RAM34). The viable cells were distinguished by their ability to exclude propidium iodide (5 ug/mL). All monoclonal antibodies were purchased from Pharmingen, San Diego, CA. Flow cytometric analysis and sorting was performed on a FACSAriaII (Becton Dickinson Immunocytometry Systems, San Jose, CA) to select propidium iodide negative, lineage negative, Sca-1 and c-kit positive and CD34 negative cells that are highly enriched in viable HSCs.
Cell cycling and apoptosis
Bone marrow cells suspended in HBSS (Gibco, Grand Island, NY) containing 2% FCS (Life Technologies) were blocked with anti-CD16/32 (clone 2.4G2, Fc Block) to prevent non-specific binding. Cells were labeled as described in the immunofluorescence staining section. Cells were fixed and permeabilized in BD Cytoperm/CytoFix buffer (BD Bioscience). Subsequent co-labeling with Ki-67 and DAPI enabled the determination of cell cycle stage and DNA content. Cells negative for Ki-67 and DAPI are in G0 phase, cells positive for Ki-67 but negative to DAPI are in G1 phase and cells positive for both markers are in S/G2/M phases of cell cycle. Apoptotic cells were stained with Annexin V and 7-AAD as previously described20. Briefly, bone marrow cells were prepared and immunofluorescently stained as described above. Fluorescein isothiocyanate-conjugated Annexin V and 7-AAD were used to identify apoptotic cells, which are Annexin V positive and 7-AAD negative. Thymocytes were used as the control to distinguish Annexin V-positive and -negative cells (Pharmingen, San Diego, CA). Three independent experiments were performed in each strain and at least 3 mice were analyzed in each experiment. All experiments were carried out on ice and completed within 1.5 hours. Flow cytometric analysis was performed on a triple-laser FACSAriaII (Becton Dickinson Immunocytometry Systems, San Jose, CA). All antibodies used in this assay were purchased from BD Pharmingen (NJ).
Competitive repopulation assay
1 × 106 donor cells were mixed with an equal number of competitor bone marrow cells (B6.SJL/BoyJ) and retro-orbitally injected into lethally irradiated (9 Gy) recipient mice. Donor cells were either derived from B.D Chr5 congenic or B6 mice (CD45.2), or derived from B6 bone marrow cells with or without Slit2 overexpression (GFP-Slit2 or GFP+) cells. Recipients were bled from the retro-orbital sinus at 4, 8 and 16 weeks post-transplantation. Erythrocytes were depleted by hypotonic lysis using NH4Cl. The leukocytes were stained in triplicate with fluorescein isothiocyanate-conjugated anti-CD45.2 monoclonal antibody (clone ALI4A2) and phycoerythrin-conjugated monoclonal antibody (Becton-Dickinson-PharMingen, San Diego, CA) specific for either B (anti-CD45R/B220; clone RA3-6B2) or T lymphocytes (anti-Thy-1.2; clone 30H12), or granulocytes (anti-Ly6G/Gr-1; clone RB6-8C5) and macrophages (anti-CD11b/Mac-1; clone M1/70). Percentages of donor (CD45.2, or GFP+)-derived PB were analyzed using a FACScan instrument (Becton-Dickinson Immunocytometry Systems, San Jose, CA). At 16 weeks post-transplant, bone marrow cells were harvested, stained for LSK cells as described above and then analyzed for donor (CD45.2 or GFP+)-derived cells in FACSAriaII.
RNA isolation
Total RNA was extracted from hematopoietic cells using the Quiagen RNeasy Mini Kit (Qiagen, Valencia, CA) according to manufacturer’s instructions. This isolation method selects total RNA using silica-membrane RNeasy spin columns. Isolated RNA was eluted in water and either used directly for microarray analysis or reversed transcribed to cDNA for quantitative real-time PCR.
Microarray analysis
Triplicate RNA samples were isolated from 80,000–137,000 LSK cells of B6, D2, B.DChr5 and D.BChr5 mice. Each of the 12 samples was reverse transcribed, purified, labeled and hybridized to 12 individual arrays on two Illumina Sentrix-Mouse 6 Whole Genome Expression Beadchips (Illumina Inc., San Diego, CA). Illumina expression arrays were processed on a fee-for-service basis at the Cincinnati Children’s Hospital Medical Center Microarray Core Facility in Cincinnati, OH. Expression was quantified with an Ilumina Bead Scanner. Quality control and gene expression analysis were performed using Illumina BeadStudio v1.5.0.34. Illumina Sentrix-Mouse 6 Whole Genome Expression Beadchip. Signal intensities corresponding to each probeset were evaluated for similarity in mean expression values. Mean and variance of normalized expression values were similar for the two chips (12 individual microarrays) with the exception of a single microarray used to analyze one of the D2 samples. This sample was excluded from further analysis. The signal intensities were then normalized using the Rank Invariant normalization method, which is recommended for evaluating gene expression across similar chips. Differences in gene expression were identified using the Illumina Custom differential expression algorithm, which accounts for: 1) sequence specific biological variation; 2) nonspecific biological variation; and 3) technical error. The data were filtered by removing probesets that were absent across all chips and considered differentially expressed if the Illumina Custom differential expression algorithm generated a mean p-value of less that 0.05 (Illumina diffscore ≤13 or >13).
Quantitative real-time PCR
Identical numbers 200,000 of cells from each of the four strains, as well as Slit2-GFP and GFP control cells, were sorted via flow cytometry. Total RNA from these cells was extracted and reverse transcribed into cDNA using Taqman Reverse Transcription Reagents (Applied Biosystems, Foster City, CA) and stored at −20 °C. Real-time PCR reactions were performed in TaqMan Universal PCR MasterMix with pre-made primer and probe mixes for mouse genes Slit2, QDPR, GPR125, Robo4 and endogenous GAPDH control (Applied Biosystems, CA) according to manufacturer’s instructions. PCR reactions were set up according to manufacturer’s instructions using TaqMan® universal PCR master mix (PN 4304437). Analyses of gene expression were performed in single reporter assays in an ABI PRISM 7700 sequence detection system (PE Biosystems, Foster City, CA).
Western blots
Cell samples were lysed at a concentration of 2 × 107 cells/ml in protein lysis buffer containing: 10 mM Tris pH7.5, 50 mM NaCl, 30 mM sodium pyrophosphate, 50 mM NaF, 5 μM ZnCl2 and 1% Triton X-100, 2.8 ug/ml aprotinin (Sigma), 1 mM phenylmethylsulfonyl fluoride (Sigma), 1 mM sodium vanadate (Na3VO4) 1 ug/ml pepstatin and 1 μg/ml leupeptin (Oncogene Research, MA). Lysate was incubated on ice for 30 min and then centrifuged at 15,000 × g for 10 min to remove debris. The resulting supernatant was then aliquoted and stored at −80 °C. For Western blot, protein lysates were thawed and mixed with running buffer and a reducing agent (Novex, San Diego, CA, per manufacturer’s instructions) and heated at 95 °C for 5 min. Samples were then analyzed by denaturing PAGE (Novex, 10% bis-Tris gel) using the equivalent of 4 × 105 cells per lane. Following electrophoresis, samples were electro-transferred onto immunobilon-P membranes (Millipore, Bedford, MA), which were subsequently blocked and probed with polyclonal rabbit anti-Slit2 Ig-G antibody at a 1:3000 dilution. Primary antibodies were detected using alkaline phosphatase-conjugated secondary antibodies (Santa Cruz Biotechnology) and electro-chemifluorescent reagent (Pharmacia Biotech) according to the manufacturer’s instructions. Blots were visualized using a Molecular Dynamics STORM 860 system and Imagequant software. Following the detection and quantification of anti-Slit2 antibody, immunobilon-P membrane was sequentially stripped in 40% methanol and the buffer containing 100 mm ß-mercaptoethanol, 2% sodium dodecyl sulfate and 62.4 mM Tris-HCl to remove electro-chemifluorescent reaction product and antibodies, respectively. The stripped membrane was re-probed with anti-actin antibody (Sigma) at 1:500,000 dilution and detected as described previously20.
Retroviral transduction of primary mouse bone marrow cells
The retroviral vector, Sfbeta 91, served as a control and the backbone for cloning of Slit2 cDNA. It contained the 5′-long terminal repeat derived from myeloproliferative sarcoma virus (MPSV) and a 3′-long terminal repeat derived from spleen focus forming virus (SFFV). The internal ribosomal entry site sequence derived from the encephalomyocarditis virus was used for simultaneous translation of gene insert and the gene for enhanced GFP. The Slit2 cDNA sequence was cloned upstream of the IRES of the Sfbeta91 vector (MPSV-IRES-GFP-SFFV) to create a recombinant Slit2-carrying vector (MPSV-Lxn-IRES-GFP-SFFV). The Slit2 cDNA was amplified from hematopoietic cells using Slit2 forward primer: 5′ATTTGCGGCCGCAAGATGAGTGGCATTGGC3′ and Slit2 reverse primer: 5′GGCCTCGAGTTAGGAGGCACATCTCGCGCA3′. Production of high-titer helper-free retrovirus was carried out by standard procedure in ectotropic Phoenix packaging cells as described previously20.
Infection of primary murine bone marrow cells
Primary mouse bone marrow cells were transduced as previously described20. Briefly, bone marrow cells were harvested from mice treated 4 days previously with 150 mg/kg body weight 5-fluorouracil (5-FU; Sigma, St. Louis, MO) and cultured for 24 h in Dulbecco’s modified eagle’s medium supplemented with 10% FBS, 1% penicillin/streptomycin (Gibco Technologies, Carlsbad, CA), 50 ng/mL recombinant mouse stem cell factor, 10 ng/mL mouse interleukin 6 (mIL-6) and 10 ng/mL mouse interleukin 3 (mIL-3; R&D Systems, Minneapolis, MN). The cells were then harvested and slowly spread on the top surface of the membrane of a Transwell insert (Corning Incorporated Life Sciences, Acton, MA) at a density of 2 × 106 cells per well. The viral supernatants were added to the Transwell inserts along with 4 μg/ml of polybrene and were cultured with cells for a further 48 h. The viral supernatant was changed 3 times during this period of time. Retrovirally-transduced, i.e. GFP positive, bone marrow cells were flow cytometrically sorted using a FACSAriaII (Becton-Dickinson) and used for CAFC assay and transplantation assays.
Functional analysis of transduced cells
GFP marker was used to select transduced hematopoietic cells. GFP positive cells infected with the empty Sfbeta vector and the Slit2-containing vector were plated in limiting dilutions on confluent layers of FBMD-1 stromal cells for CAFC analysis. CAFC analysis was performed as previously20 described to measure the effects of Slit2 expression on HSC and HPC numbers. Competitive repopulation assays with GFP positive cells were performed by co-transplantation of 2 × 105 GFP+ cells with equal numbers of CD45.1 helper cells to two groups of lethally irradiated mouse recipients (10 recipients of GFP cells and 10 recipients of Slit2-GFP cells). Relative engraftment was determined by measuring the level of GFP positive cells in the peripheral blood of each recipient mouse 4–16 weeks post-transplant. In the limiting-dilution competitive repopulation assay as described previously20, graded numbers (6,000; 20,000; 60,000) of B6 GFP+ or B6 Slit2 GFP+ cells (CD45.2) were admixed with a radio-protective dose (2 × 105) of competitor cells (CD45.1) and injected intravenously into lethally irradiated (900 Gy) CD45.1 recipient mice. Relative engraftment was determined by measuring the level of GFP positive cells in the peripheral blood of each recipient mouse 12 weeks post-transplant as described above. The frequencies of long-term HSCs (CRU) were calculated from the proportions of negative recipients in each cell dose group, in which <1% of the circulating B, T and myeloid cells were regenerated by CD45.2 “test” stem cells, by using L-Calc software (StemCell Technologies Inc., Vancouver, BC).
Mapping of eQTL for Slit2 in BXD mice
We queried gene expression data in hematopoietic stem cells of 23 BXD recombinant inbred strains using the UMCG Hematopoietic Stem Cells Illumina database in Genenetwork (http://genenetwork.org/webqtl/main.py) to map regulatory elements governing the expression of Slit2 in these strains. Data for eight Illumina probes was available through in this database. We selected Illumina probe ILM1940037 which corresponded to the probe identified as differentially expressed in our microarray analysis and performed Interval mapping across the entire genome to identify regulators of Slit2 expression or eQTL.
Statistical analysis
Data were analyzed by either Student t-test with P < 0.05 (two-tail), or one-way ANOVA. All experimental methods were run in accordance with relevant guidelines.
Additional Information
Accession code: GEO Accession# GSE21896.
How to cite this article: Waterstrat, A. et al. Quantitative trait gene Slit2 positively regulates murine hematopoietic stem cell numbers. Sci. Rep. 6, 31412; doi: 10.1038/srep31412 (2016).
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Acknowledgements
The authors acknowledge significant contributions made by Hua Li, Na Ren and Arnold Stromberg in statistical analysis of microarray data and helpful comments from James Geddes, Rodney Guttmann, Mike Reid and Rob Williams. We also acknowledge the editorial assistance of the University of Kentucky Markey Cancer Center Research Communications Office. The work is supported by the National Center for Advancing Translational Sciences, National Institutes of Health, through grant number KL2TR000116 (YL), the National Heart, Lung and Blood Institute of the National Institutes of Health under Award Number RO1HL124015 (YL), National Institutes of Health R36 AG029669 (AW), National Cancer Institute, grant number P30 CA177558, National Institute of Health and Start-up funding from Markey Cancer Center at the University of Kentucky (YL).
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A.W. performed the majority of experiments. K.R., H.G. and Y.L. performed some experiments. A.W. and Y.L. wrote the main manuscript text. A.W., Y.L. and H.G. reviewed the manuscript.
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Waterstrat, A., Rector, K., Geiger, H. et al. Quantitative trait gene Slit2 positively regulates murine hematopoietic stem cell numbers. Sci Rep 6, 31412 (2016). https://doi.org/10.1038/srep31412
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DOI: https://doi.org/10.1038/srep31412
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