Despite the pathological importance of fibrin clot formation, little is known about the structure of these clots because X-ray and nuclear magnetic resonance (NMR) analyses are not applicable to insoluble proteins. In contrast to previously reported anti-fibrin monoclonal antibodies (mAbs), our anti-fibrin clot mAb (clone 102–10) recognises an uncovered region that is exposed only when a fibrin clot forms. The epitope of the 102–10 mAb was mapped to a hydrophobic region on the Bβ chain that interacted closely with a counterpart region on the γ chain in a soluble state. New anti-Bβ and anti-γ mAbs specific to peptides lining the discovered region appeared to bind exclusively to fibrin clots. Furthermore, the radiolabelled 102–10 mAb selectively accumulated in mouse spontaneous tumours and immunohistochemistry using this mAb revealed greater fibrin deposition in World Health Organization (WHO) grade 4 glioma than in lower-grade gliomas. Because erosive tumours are apt to cause micro-haemorrhages, even early asymptomatic tumours detected with a radiolabelled 102-10 mAb may be aggressively malignant.
Fibrinogen is composed of two pairs of polypeptide chains, including the Aα, Bβ and γ chains, linked by disulfide bonds. Fibrinogen is involved in fibrin clot formation, which is the end result of blood coagulation1,2,3,4. Fibrin clot formation is the most dynamic and important event in haemostasis and thrombosis, which accompany injury5, heart6 or brain7 infarction, severe inflammation8 and cancer invasion9 or metastasis10.
Tumours that are erosive are also more destructive and thus result in fibrin clot formation. When cancer clusters erode adjacent normal or tumour vessels, micro-haemorrhage may occur and fibrin clots are immediately formed in situ to stop the bleeding. These fibrin clots are subsequently replaced by collagenous stroma, similar to the process of normal wound healing11. Therefore, the “malignant cycle of blood coagulation” has been postulated to generate versatile cancer stroma, leading to cancer invasion into vessels, haemorrhage, fibrin clot formation and replacement with collagenous tissue. Additionally, most human tumours possess abundant stroma12,13, in contrast to human haematologic malignancies and tumour xenografts in mice, which have less interstitial tissue. Generally, more invasive cancers possess more abundant cancer stroma, most likely due to frequent haemorrhages at many places within or adjacent to the tumour tissue. In an invasive cancer, fibrin clot formation persists asymptomatically as long as cancer cells survive and expand from a tiny tumour to the advanced stage12,13.
To develop our anti-human fibrin mAb, we crushed a human fibrin clot and injected the suspension in saline into mice13. Consequently, we obtained a monoclonal antibody (mAb) (clone 102–10) that could distinguish fibrin clots from fibrinogen, soluble fibrin (precursor of fibrin clot14) and D-dimer (degradation product of fibrin clot15), which are all soluble proteins (Fig. 1a and Supplementary Fig. 1a). Although some anti-fibrin mAbs have been developed, none react exclusively with fibrin clots; rather, they also react with fibrinogen, soluble fibrin, or D-dimer2,16,17,18,19,20,21. Thus, the generation of a mAb that can distinguish fibrin clots from fibrinogen, soluble fibrin and D-dimer represents a major breakthrough because these proteins share common amino acid sequences. The specificity of the 102–10 mAb differed from existing anti-fibrin mAbs (NYB-T2G122,23 and MH-120), as the 102–10 mAb reacted only with fibrin clots (Supplementary Fig. 1b). Enzyme-linked immunosorbent assay (ELISA) also demonstrated that the 102–10 mAb specifically reacted with the fibrin clot in a dose-dependent manner, whereas it did not react with fibrinogen or D-dimer (Supplementary Fig. 1c).
Epitope mapping for the 102–10 mAb revealed that it reacted with fibrinogen under reducing and heat-denatured conditions in ELISA and western blot assays (Supplementary Fig. 1d–e). This result indicated that the epitope was exposed under reducing conditions. Comparing Coomassie brilliant blue (CBB) staining with the western blot results revealed that only the Bβ chain of fibrinogen reacted with the 102–10 mAb (Fig. 1b). To confirm the location of the epitope of the 102–10 mAb, the fibrinogen Bβ chain was digested with lysyl endopeptidase and a Bβ chain derived peptide of approximately 10 kDa was obtained, which was recognised by the 102–10 mAb (Fig. 1c). According to the protein sequence result, this obtained peptide consisted of residues 149–234 of the Bβ chain. To confirm the epitope's sequence, five synthetic peptides were prepared with the following regions corresponding to synthetic peptides: No. 1 (Bβ149–178), No. 2 (Bβ179–208), No. 3 (Bβ209–234), No. 4 (Bβ171–186) and No. 5 (Bβ201–216). Competitive inhibition tests revealed that only one of these peptides (No. 5) inhibited the binding of the 102–10 mAb to fibrin clots, as measured by ELISA (Fig. 1d). The sequence of the No. 5 synthetic peptide was CNIPVVSGKECEEIIR, which constitutes a hydrophobic region of residues 201–216 of the Bβ chain. In view of the structure of fibrinogen, we could deduce that the epitope of the 102–10 mAb interacted with residues 206–220 of the γ chain of fibrinogen (Fig. 1e: Protein Data Bank (PDB) code 3GHG24). Interestingly, these 2 amino acid sequences are completely conserved in mammals, birds, amphibians and fish (Basic Local Alignment Search Tool (BLAST)), which suggests that these sites have major importance for blood coagulation across species.
Identifying the epitope on a fibrin clot is generally difficult because X-ray analysis and NMR are not applicable to insoluble proteins. However, we identified the precise peptide sequence of the epitope using the 102–10 mAb against the fibrin clot and discovered a unique region in which certain sequences on the Bβ chain (containing the epitope of the 102–10 mAb) and γ chains of fibrinogen were hidden. To confirm this finding, we prepared a peptide antigen corresponding to the epitope region of the 102–10 mAb and a peptide antigen corresponding to the counterpart region on the γ chain. We immunised mice with these 2 synthetic peptides and succeeded in establishing mAbs against these regions on the Bβ chain (anti-Bβ mAb) and the γ chain (anti-γ mAb). Both the anti-Bβ mAb and the anti-γ mAb specifically recognised fibrin clots (Fig. 2a–b). These findings imply that a conformational change occurs in the region that acts as an epitope for each mAb when fibrinogen develops into a fibrin clot, which produces the unique region (Fig. 2c). To characterise this unique region, a competition ELISA was conducted using the peroxidase-conjugated anti-Bβ mAb, peroxidase-conjugated anti-γ mAb and each corresponding cold mAb. The binding of peroxidase-conjugated anti-Bβ mAb to the fibrin clot was blocked by the cold anti-Bβ mAb but not by the cold anti-γ mAb. Moreover, the binding of peroxidase-conjugated anti-γ mAb to the fibrin clot was blocked by the cold anti-γ mAb but not by the cold anti-Bβ mAb (Supplementary Fig. 2). These findings indicate that the newly identified unique region within fibrin clots was large enough for both the anti-Bβ and anti-γ mAbs to bind simultaneously (Fig. 2d). Further structural analysis of this unique region revealed that hydrophobic interactions and ionic and hydrogen bonds are involved in the interaction between the Bβ chain and γ chain of this particular region (Fig. 2e–g). The dynamic force exerted upon the formation of a fibrin clot is expected to break these bonds, resulting in the appearance of the unique region within the clots.
Because this uncovered region is uncovered only when a fibrin clot forms and the amino acid sequence of the epitope of the 102–10 mAb is completely conserved in mammals, the 102–10 mAb and other mAbs developed against human fibrin clots cross-reacted with mouse or rat fibrin clots. Therefore, we investigated the fate of fibrin clots in mice or rats using disease models, such as models of cerebral infarction, incisional wounds and arthritis (Fig. 3a). These results indicated that fibrin clot formation occurred only in the acute phase of non-malignant diseases, including thrombosis, inflammation and trauma and these clots virtually disappeared within 2 to 3 weeks and were substituted by collagen in the late phase. We next synthesised a positron emission tomography (PET) probe of 89Zr-labelled 102–10 (Fig. 3b), which was injected into mice bearing chemically induced spontaneous cutaneous tumours generated using 7,12-dimethylbenz[a]anthracene (DMBA) as an initiator and phorbol 12-myristate 13-acetate (PMA) as a promoter25. This spontaneous tumour was selected as an appropriate experimental model to evaluate our immuno-probe because it exhibits remarkable fibrin deposition and abundant interstitial tissue, similar to clinical human cancers and unlike human tumour xenografts in mice26. The 102–10 mAb probe showed clear and more specific accumulation in tumours compared to cetuximab (control), as assessed by computerized tomography (CT) scan (Fig. 3c, Supplementary Fig. 3). Next, we histologically evaluated the specificity of the 102–10 mAb probe within the tumour tissue. Haematoxylin and eosin (HE) staining showed that the tumour consisted of areas with basophilic tumour cells and eosinophilic tumour stroma and the accumulation of the 102–10 mAb probe was more pronounced in fibrin clot-positive tumour stroma (Fig. 3d). These PET/CT data from mouse experiments can be reasonably extrapolated to humans because the 102–10 mAb can recognise both human and mouse fibrin clots and humans and mice show common features of fibrin deposition.
To evaluate the reactivity of the 102–10 mAb to intravital fibrin clots under various conditions in humans, normal tissue and tissues from non-malignant and malignant diseases were stained with the 102–10 mAb. Strong fibrin deposition was observed in almost all cancer tissues, including brain, lung, pancreatic and colorectal cancers, but not in normal tissues (Fig. 4a and Supplementary Table 1). In cases of non-malignant disease, fibrin clots were detected at the onset of cerebral infarction and cardiac infarction or in cases of serious inflammation, such as acute pancreatitis (Supplementary Table 1). However, no clear fibrin clots were detected in the late or chronic phases of non-malignant conditions following the digestion of fibrin clots by plasmin and replacement by collagenous tissue (Fig. 4b).
Erosive types of cancer are known to be more destructive, resulting in higher fibrin clot deposition. In this context, we closely investigated fibrin deposition in surgically resected samples of glioma, one of the most erosive tumour types. Immunohistochemistry was conducted for WHO-classified glioma tissue samples and the level of fibrin deposition was graded into three subgroups: (−) fibrin clot not detectable, (+) faint but clear deposition, or (++) strong heterogeneous or diffuse deposition (Fig. 4c). Fibrin deposition was observed in 100% (20/20) of grade 4 gliomas (glioblastoma multiforme (GBM)), with strong fibrin deposition observed in 65% of cases (13/20). In grade 3 gliomas, fibrin deposition was observed in 40% of cases (8/20), with strong fibrin deposition observed in 10% of cases (2/20). In grade 1/2 gliomas, fibrin deposition was observed in 60% of cases (12/20) and strong fibrin deposition was observed in 10% of cases (2/20) (Fig. 4d).
Combining previous lines of clinicopathological evidence with our present data, even tiny and asymptomatic tumours will likely become aggressively malignant if they are detected by PET/CT using the radiolabelled 102–10 mAb. The deposition of fibrin in non-malignant disease is inevitably accompanied by various symptoms related to the particular pathology, such as infarction or inflammation. Conversely, tumour-related fibrin deposition is not associated with any symptoms, even in patients with stage 4 disease. When a cancer diagnosis is accompanied by symptoms, such as pain or macroscopic bleeding, the cancer likely involves sensory nerves and the destruction of bone and large blood vessels, which is generally observed in patients with end-stage cancer. Therefore, earlier detection of cancers at the asymptomatic stage using mAbs is important to achieve a favourable clinical outcome27.
The biological significance of tumour fibrin clots has been gradually elucidated, as several growth factors that preserve the fibrin matrix have been shown to promote tumour cell growth, angiogenesis28 and metastasis27,29. Conversely, the role of fibrin clots in the tumour stroma has yet to be definitively characterised. Here, we discovered an uncovered region that develops in the fibrin clot during its formation and we generated mAbs that bound exclusively to fibrin clots. The specificity of the 102–10 mAb to fibrin clots was verified by the present ELISA and western blot (Figure 1a, supplementary Fig. 1c and 4). Therefore, the 102–10 mAb may not be neutralised by fibrinogen, soluble fibrin, or D-dimer in the bloodstream due to its unique properties.
Our immunohistochemical and PET/CT findings using these newly developed anti-insoluble-fibrin mAbs, including 102–10, further demonstrated that these mAbs could be used to detect high-grade, aggressive, malignant tumours. Further development of such a method to detect fibrin clots is therefore desirable from an oncological perspective.
Production of the 102–10 mAb
We developed our anti-human fibrin mAb by converting fibrinogen (Sigma, St Louis, MO) into a fibrin clot via thrombin (Sigma) cleavage. The fibrin was frozen in liquid nitrogen and crushed and the crushed fibrin was then suspended in saline and used to immunise mice. Lymph node cells from an immunised mouse were fused with myeloma cells (P3U1) and specific antibody-producing hybridoma clones were selected by ELISA as described previously13.
Enzyme-linked immunosorbent assay (ELISA)
The reference standards for soluble fibrin and D-dimer were obtained from Sekisui Medical (Tokyo, Japan). One microgram of antigen was immobilised onto a 96-well plate for 12 hours. The fibrinogen-immobilised plates were then treated with a thrombin solution at 37°C for 1 hour to prepare the fibrin clot plates, as described previously20. The wells were then blocked using N102 (Nichiyu, Tokyo, Japan) for 3 hours. Subsequently, the plates were incubated in 1 μg/mL peroxidase-conjugated mAb 1 hour and the wells were washed with Tris-buffered saline (TBS) containing 0.05% Tween 20 (TBS-T). Finally, the mAbs bound to the wells were visualised using a 1-Step Slow TMB-ELISA (Thermo, Waltham, MA, USA) as a substrate for 30 minutes.
The mAbs were first conjugated to peroxidase (Dojindo, Kumamoto, Japan). One microgram of fibrinogen or its proteolytic product sample was obtained by Sodium dodecyl sulphate polyaclylamide gel electrophoresis (SDS-PAGE) under reducing, heat-shocked, or untreated conditions. The proteins were then electrophoretically transferred to polyvinylidenedifluoride (PVDF) membranes (Bio-Rad, Hercules, CA, USA) using the Trans-Blot Turbo transfer machine (Bio-Rad). The membranes were then placed in a protein detection system (SNAP i.d.; Millipore, Billerica, MA, USA) and blocked with phosphate-buffered saline (PBS) containing 0.3% Difco skim milk (Becton Dickinson, Franklin Lakes, NJ, USA) and 0.1% Tween 20 (PBS-T, Sigma). The membranes were then incubated in 2 μg/mL peroxidase-conjugated mAbs with 0.3% skim milk in 0.1% PBS-T for 10 min at room temperature. Subsequently, the membranes were washed with TBS-T. Finally, the proteins on the membrane were visualised using ECL prime (GE Healthcare, Piscataway, NJ, USA) as a substrate. After analysis in a ChemiDoc imager (Bio-Rad), the membrane was stained with Quick CBB (Wako Pure Chemical Industries, Osaka, Japan).
Isolation of fibrinogen Bβ chain
Five micrograms of fibrinogen were separated by SDS-PAGE under reducing conditions. The polyacrylamide gel was then stained with EZ Stain reverse (ATTO, Tokyo, Japan). The fibrinogen Bβ chain fraction was cut out from the polyacrylamide gel, followed by extraction of the protein using an Atto prep MF column (ATTO).
Limited digestion with lysyl endopeptidase and protein sequencing
Lysyl endopeptidase (Wako Pure Chemical Industries) digestion of the fibrinogen Bβ chain protein was carried out in PBS (pH 7.4) with a protein:enzyme ratio (w/w) of 45:1 at 37°C for 6 hours and the digested peptide was separated by SDS-PAGE electrophoresis under reducing conditions. The fragments were detected by western blot and the minimum positive band (approximately 10 kDa) was obtained using the method described above. The amino acid sequence of the 10-kDa peptide was determined using a PPSQ-33A protein sequencer (Shimadzu, Kyoto, Japan).
Inhibition of 102–10 binding to fibrin clots using a series of synthetic peptides
Five synthetic peptides [No. 1 (Bβ149–178), No. 2 (Bβ179–208), No. 3 (Bβ209–234), No. 4 (Bβ170–186) and No. 5 (Bβ201–216)] were prepared using the amino acid sequence of the 10-kDa peptide (Sigma). A PBS solution containing 1 μg/mL peroxidase-conjugated 102–10 was then mixed with diluted synthetic peptides (0.01–100 μM) and the mixture was incubated for 1 hour at 37°C. After discarding the blocking reagent, 100 μL of the mixture was applied to a fibrin-coated 96-well plate17. Subsequently, the wells were washed with TBS-T and the antibodies in the wells were visualised using the 1-Step Slow TMB ELISA as a substrate for 5 minutes.
Producing the anti-Bβ chain and anti-γ chain mAbs
The crystal structure of fibrinogen (3GHG) was obtained from the RCSB Protein Data Bank (http://www.rcsb.org/pdb/home/home.do) and visualised using PyMOL ver1.3r1 edu (http://www.pymol.org/pymol). To produce new antibodies that recognise epitope regions of the fibrin clot Bβ chain (CNIPVVSGKECEEIIR) or γ chain (KNWIQYKEGFGHLSP), recombinant epitope protein was produced from pET21b (Novagen, Darmstadt, Germany) and fibrinogen Bβ chain DNA or fibrinogen γ chain DNA (Origene Technology, Rockville, MD). Six-week-old BALB/cAnNCrlCrlj mice (Charles River Japan, Yokohama, Japan) were immunised intraperitoneally (i.p.) with an emulsion of Freund's complete adjuvant (DIFCO, Franklin Lakes, NJ, USA) and a saline solution containing 50 μg of the Bβ chain of the epitope peptide with a 4M-tag (Bio Matrix Research, Inc., Chiba, Japan). Three to five successive booster injections were administered i.p. at 2-week intervals using the same amount of antigen in an adjuvant system (Sigma). A final boost was provided by administering the same amount of antigen intravenously. An antibody recognising the γ chain of the fibrin clot was produced by ITM Co. Ltd (Matsumoto, Japan). The specificity of the obtained mAbs against the epitope regions of the Bβ and γ chains was evaluated by ELISA.
Hydrophobic, ionic and hydrogen bonds between the Bβ chain and γ chain in the unique region of fibrin clots
Electrostatic surface representations (−5 kT/e, red, to +5 kT/e, blue) of the Bβ chain and γ chain were generated using the APBS software package (https://sites.google.com/a/poissonboltzmann.org/software/apbs) and the images of all molecular structures were displayed in PyMOL ver1.3r1 edu.
Non-malignant human tissues were obtained from Fukushima Medical University. Glioma samples were obtained from Kumamoto University. Immunohistochemical studies in human tissue samples were conducted on surgically resected tissue samples and tissue samples obtained from autopsy. All samples were collected according to an institutional review board-approved protocol. Tissue sections from paraffin-embedded tissue samples were prepared and heat-induced antigen retrieval was performed at 120°C for 10 minutes in 10 mM citrate (pH 6). After blocking with 5% skim milk in PBS, the sections were incubated with the 102–10 mAb for 1 to 2 hours at room temperature or overnight at 4°C. After washing with PBS, the sections were then incubated with peroxidase-conjugated anti-human IgG secondary antibody (MBL Co., Ltd., Nagoya, Japan) for 60 minutes. The reaction was visualised using DAB (Dako, Glostrup, Denmark) and the slides were counterstained with haematoxylin.
PET/CT imaging and autoradiography using 89Zr-labelled 102–10
The 102–10 mAb was conjugated to p-isothiocyanatobenzyl-desferrioxamine B (DF) as previously described30. The conjugation ratio of DF to IgG was estimated to be 1.0 to 1.3, as determined by size-exclusion chromatography using a PD10 column (GE Healthcare). The nonconjugated chelate was removed using a Sephadex G-50 spin column (GE Healthcare). 89Zr-oxalate was produced using a cyclotron at the National Institute of Radiological Sciences (Chiba, Japan) as previously described31. The DF-conjugated 102–10 mAb (100 μg in 20 μL of PBS) was incubated with 5.0 to 5.6 MBq of 89Zr-oxalate (3.7–5.6 GBq/mL, pH 7–9) for 1 hour at room temperature. The radiolabelled 102–10 mAb was purified using a Sephadex G-50 spin column. The radiochemical yield of 89Zr-labelled IgG was between 73 and 96%, the radiochemical purity was between 96 and 98% and the specific activity was between 37 and 44 kBq/μg as determined by thin-layer chromatography using 50 mM diethylene triamine pentaacetic acid (DTPA, pH 7) as the mobile phase.
Mice were injected with approximately 3.7 MBq of 89Zr-labelled 102–10 mAb into the tail vein. The injected protein dose was adjusted to 100 μg per mouse by the addition of unlabelled antibody. The PET data were acquired for 10 to 20 minutes using a small-animal PET system (Inveon, Munich, Germany) under isoflurane anaesthesia. Body temperature was maintained at 37°C using a lamp and a heating pad during the scan. The images were reconstructed using 3D maximum a posteriori (18 iterations with 16 subsets, β = 0.2 resolution) without attenuation correction. The tracer uptake was expressed as the percentage of the injected dose per gram of tissue (% ID/g). After PET scanning, the CT images were acquired with an X-ray source set at 90 kVp and 200 μA using a small-animal CT system (R_mCT2, Rigaku, Tokyo, Japan). After imaging, the mice were euthanised and the tumours were excised and quickly frozen in an optimal-cutting-temperature (OCT) compound (Sakura Finetek Japan, Tokyo, Japan). The dried sections (20 μm thick) were exposed to an imaging plate (Fuji Film, Tokyo, Japan) and then stained with HE.
Chemical skin carcinogenesis was induced as previously described26,32,33,34. Briefly, 7,12-dimethylbenz[a]anthracene (DMBA; 250 μg/mL in acetone; Sigma) was applied once to the shaved dorsal skin of the mice as an initiator. After 1 week, phorbol 12-myristate 13-acetate (PMA; 25 μg/mL in acetone; Sigma) was applied weekly (for a total of 32 times) as a promoter. All animal procedures and experiments were approved by the Committee for Animal Experimentation of the National Cancer Centre, Tokyo Japan.
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This work was supported by the National Cancer Centre Research and Development Fund (Y.M.); the Funding Program for World-Leading Innovative R&D in Science and Technology (FIRST Program) (Y.M.); the Third Term Comprehensive Control Research for Cancer from the Ministry of Health, Labour and Welfare of Japan (Y.M.); a Grant-in-Aid for Scientific Research or Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology; the Princess Takamatsu Cancer Research Fund (Y.M.); and the Japanese Foundation for Multidisciplinary Treatment of Cancer (Y.M.). We thank Dr. S. Iwanaga for his expertise. We also thank Ms. M. Takigahira for her assistance with the animal experiments, Mrs. H. Koike and Mrs. M. Araake-Mizoguchi for their assistance with producing the 102–10 mAb and Mrs. K. Shiina for secretarial support.
The authors declare no competing financial interests.
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Hisada, Y., Yasunaga, M., Hanaoka, S. et al. Discovery of an uncovered region in fibrin clots and its clinical significance. Sci Rep 3, 2604 (2013). https://doi.org/10.1038/srep02604
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