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Reply to: Absence of evidence that Slc12a8 encodes a nicotinamide mononucleotide transporter

Nature Metabolism volume 1, pages 662–665 (2019)Cite this article

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The Original Article was published on 12 July 2019

replying to Mark S. Schmidt and Charles Brenner Nature Metabolism https://doi.org/10.1038/s42255-019-0085-0 (2019)

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Fig. 1: Analysis of NMN using HPLC and LC–MS/MS.
Fig. 2: Hypercarb HPLC chromatograms of NMN in extracts from control and FK866-treated NIH3T3 cells and mouse primary hepatocytes.

References

  1. Schmidt, M. S. & Brenner, C. Absence of evidence that Slc12a8 encodes a nicotinamide mononucleotide transporter. Nat. Metab. https://doi.org/10.1038/s42255-019-0085-0 (2019).

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Author information

Authors and Affiliations

  1. Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA

    Alessia Grozio, Kathryn Mills, Kyohei Tokizane, Hanyue Cecilia Lei & Shin-ichiro Imai

  2. Center for Human Nutrition, Division of Geriatrics and Nutritional Science, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA

    Jun Yoshino

  3. Department of Experimental Medicine, Section of Biochemistry, and Center of Excellence for Biomedical Research, University of Genova, Genova, Italy

    Santina Bruzzone & Giovanna Sociali

  4. Department of Genetics, Washington University School of Medicine, St. Louis, MO, USA

    Yo Sasaki

  5. Mitchell Cancer Institute, University of South Alabama, Mobile, AL, USA

    Marie Migaud

Authors
  1. Alessia Grozio
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  2. Kathryn Mills
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  3. Jun Yoshino
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  4. Santina Bruzzone
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  6. Kyohei Tokizane
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  7. Hanyue Cecilia Lei
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  8. Yo Sasaki
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  9. Marie Migaud
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  10. Shin-ichiro Imai
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Contributions

S.I. and A.G. mainly wrote the correspondence. J.Y. conducted the experiments and analyses discussed in Fig. 1. A.G. performed the experiments and analyses discussed in Fig. 2. K.M., S.B., G.S., K.T., H.C.L., M.M. and Y.S. contributed to the preparation of the correspondence.

Corresponding author

Correspondence to Shin-ichiro Imai.

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Competing interests

A.G. and S.I. are inventors on a patent (PCT/US18/46233) about the Slc12a8 NMN transporter, whose applicant is Washington University; it has been licensed by Teijin Limited. The other authors declare no competing interests.

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Grozio, A., Mills, K., Yoshino, J. et al. Reply to: Absence of evidence that Slc12a8 encodes a nicotinamide mononucleotide transporter. Nat Metab 1, 662–665 (2019). https://doi.org/10.1038/s42255-019-0086-z

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