Abstract
The emergence of proteins and their interactions with RNAs were a key step in the origin and early evolution of life. The abiotic synthesis of peptides has been limited in short amino acid length and is favored in highly alkaline evaporitic conditions in which RNAs are unstable. This environment is also inconsistent with estimated Hadean Earth. Prebiotic environments rich in boron are reportedly ideal for abiotic RNA synthesis. However, the effects of boron on amino acid polymerization are unclear. We report that boric acid enables the polymerization of amino acids at acidic and near-neutral pH levels based on simple heating experiments of amino acid solutions containing borate/boric acid at various pH levels. Our study provides evidence for the boron-assisted synthesis of polypeptides in prebiotically plausible environments, where the same conditions would allow for the formation of RNAs and interactions of primordial proteins and RNAs that could be inherited by RNA-dependent protein synthesis during the evolution of life.
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Introduction
RNAs are biopolymers that can carry genetic information and catalyze biological reactions1. Interactions between proteins and RNAs support essential biological processes, including transcription, translation, and regulation of gene expression2. The origin of these interactions was a key step in the origin of life and is thought to have occurred at the beginning of the RNA world or the transition from the RNA world to DNA–protein systems2,3,4,5,6,7,8.
Various proteinogenic amino acids would have been generated by terrestrial synthesis and extraterrestrial delivery to prebiotic Earth9,10. Prebiotic peptide synthesis has been investigated for decades in different geological settings, including volcanic geothermal fields, hydrothermal fields, sea-floor sediments, and tidal flats11,12,13,14,15,16. The effects of minerals, salts, ions, and pH have also been investigated11,12,13,14. Under highly alkaline conditions, peptide synthesis was favored, but the synthesized peptides were limited in terms of 20-mer oligopeptides (Gly20), unless the amino acids were chemically activated8. Highly alkaline conditions are not compatible with RNA synthesis due to its low stability. Thus, the origins of these essential biopolymers have been regarded separately. Another approach of the prebiotic peptide synthesis is the multistep coupling of an aminonitrile with thioacetic acid, although the presence of thioacetic acid on prebiotic Earth has not been clear, and the products are limited in Gly617.
Previous studies found that borate can bind ribose and selectively stabilize it among aldopentoses, e.g., 18,19,20. Boric acid is important prebiotic reagent for regioselective phosphorylation of ribose and nucleosides to form ribonucleoteds21,22,23,24,25. Furthermore, boric acid can fix ribose in furanose in ribonucleoside synthesis23. Therefore, environments rich in borate and boric acid were potentially ideal for the formation of RNA on prebiotic Earth26. A potential effect of borate on prebiotic peptide formation was previously discussed27, but it remains unclear. In this study, we report the effect of boric acid in the polymerization of Gly. This effect was substantial under acidic and neutral evaporative conditions, and thus boric acid could promote prebiotic peptide synthesis in the same environment where RNAs are stable.
Results and discussion
Formation of polypeptides and the effects of boron
Here, we report the abiotic polymerization of amino acids (up to Gly39) by simple thermal evaporation of a near-neutral amino acid solution (pH 6 and 8) containing boric acid at 130 °C for 200 h (Fig. 1, Supplementary Figs. 1–3 and Supplementary Tables 1 and 2). The yields of short Gly peptides and the lengths of detectable long peptides were dependent on the amount of boric acid in the starting material (Fig. 2). In the absence of boric acid, the longest product peptides were Gly9 and Gly13 under the same pH and temperature. The total yield of short oligomers (i.e., Gly2–5 and diketopiperazine [DKP]) was limited to 0.00025 times that of the thermal evaporation experiment with boric acid at pH 6 (Fig. 3). The yields of short oligomers were comparable under different pH conditions in the presence of boron species, but the formation of long oligomers was the most substantial under near-neutral conditions (pH 6–8) at 130 °C (Fig. 3a–e). In contrast, in the absence of boron species, both the yields and lengths of oligomers were higher under acidic and alkaline conditions than under neutral conditions (Fig. 3f–j). The catalytic effects of boron species on peptide synthesis were also shown under an acidic condition at 90 °C (Supplementary Fig. 4), whereas this effect was not shown under the acidic condition at 130 °C. Substantial conversion of Gly to a black by-product under the high-temperature acidic condition would have overwhelmed the boron-assisted peptide synthesis at 130 °C, while this by-product synthesis was negligible at 90 °C (Supplementary Figs. 5 and 6). Wet-dry cycles did not provide significant effects on the yields and the length of product Gly peptides (Supplementary Fig. 7). The enhancement of peptide synthesis by boric acid was also confirmed for alanine, although longer reaction durations will be required for the formation of polyalanine (Supplementary Fig. 8).
Organic boron species and boric acid form borate esters with carboxylic acids and catalyze the formation of amide bonds with amines by refluxing their water-free organic solvents28,29,30. We investigated the formation of esters between Gly and borate (Gly-B) and Gly and boric acid (Gly-BA) in starting solutions of different pH. It was found that Gly and boron hydroxides formed Gly-BA in the near-neutral solution and Gly-B in the acidic, near-neutral, and alkaline solutions (Fig. 4a, b). 11B-nuclear magnetic resonance (NMR) of the starting solutions showed that the speciation of bulk boron hydroxides was typical in that borate and boric acid dominated at pH >9 and <9, respectively (Fig. 4c). However, the speciation of boron that formed esters with Gly differed from that of typical boron hydroxide solutions, which is consistent with the findings of a previous study31. It remains unclear whether Gly-B or Gly-BA is a more efficient reaction species for peptide synthesis. We also conducted FT-IR analysis of dried starting materials (Supplementary Fig. 7). A typical peak reported as an asymmetric νasym(BO)/BO4 stretch of boric acid appearing in the 1190 cm−1 region was shifted to 1227 cm−1 region in the analysis of a precipitate of a solution containing Gly and boric acid, supporting the formation of an ester compound as investigated in a previous study32.
The reactions under highly acidic and alkaline conditions without boron converted 70% and consumed 90% of the initial Gly, generating brown to black by-products (Fig. 3j and Supplementary Figs. 7 and 8), while the reactions under neutral conditions with boron converted 50% and consumed 50% of the initial Gly (Fig. 3c). Thus, boron-assisted peptide synthesis is a highly effective reaction with limited formation of by-products. When the boron esters react with Gly to form a peptide bond, borate and boric acid are released as the same form of boron species (Fig. 4a). Thus, small amounts of boron species can continuously catalyze peptide synthesis.
Implication to Hadean Earth
Amino acid oligomerization is favored under highly alkaline and acidic conditions12,14,15. Alkaline and acidic environments are common around submarine hydrothermal areas33,34,35. However, dehydration during the formation of a peptide bond does not effectively occur in water-rich environments, and RNAs are not stable in highly acidic and alkaline conditions. A recent finding suggested that neutral environments favored the polymerization of ribonucleotide to form primordial RNA36. The pH of the Hadean ocean is unclear, but the pH of the early Archean ocean is estimated to have been near-neutral (pH ~6.5–7)37. This pH is compatible with amino acid polymerization in the presence of boric acid, as shown in this study. Given the presence of small land environments on the Hadean Earth38, evaporitic basins would have been common. Boron occurs as a borosilicate mineral, tourmaline, in 3.8-Ga old metasediments of Isua Greenland39. Boron isotope compositions in the tourmaline suggest that dissolved borate and boric acid were enriched in isolated basins on early Archean Earth, and may date back to Hadean Earth26,39. These isolated basins would also have accumulated amino acids and other organic compounds compared with the open ocean. Thus, evaporitic environments enriched in amino acids and boric acid would have been ideal for the formation of peptides on Hadean Earth. The temperatures of these environments would have been lower than those in our experiments, but the catalytic effects of boron species to promote Gly oligomerization were evident even at a lower temperature, i.e., 90 °C (Supplementary Fig. 4). Small proteins—composed of a limited amount of amino acids (e.g., <100)—have various biological functions40,41. The length of the peptides formed in this study (i.e., 39 amino acid residues) is typical of small proteins. The catalytic effect of boric acid is evident for Gly and Ala, and thus it may catalyze the polymerization of multiple amino acids, although this is a subject of future investigation.
The near-neutral pH at which boron-assisted polypeptide synthesis occurs also favors the stability of RNA, unlike the highly alkaline and acidic experimental conditions of previous studies12,13,14,15. Given that boron species can promote abiotic RNA synthesis, it is likely that the same environments of prebiotic Earth allowed for the interaction of abiotic polypeptides and RNAs, which would have facilitated the formation of these complex polymers with various functions. Some riboswitches are amino acids, and they can be associated with translation. This suggests that RNA could have interacted under prebiotic environments with amino acids or small peptides42. Proto-peptides composed of several amino acids and hydroxy acids are known to increase the stability of RNAs7. The core domain of ancient RNA polymerase might have been composed of ∼40 amino acids43. Furthermore, some peptides can activate the functions of RNA polymerase ribozyme6,44. Therefore, evaporitic basins on Hadean Earth might have provided primordial functional polymers composed of peptides and RNAs that could be inherited by RNA-dependent protein synthesis during the evolution of life.
Materials and methods
All experiments were conducted using glass vials placed in an aluminum block in an electric furnace. The size of the glass vials was 1.5 mL, 32 mm in height, 6 mm upper interior diameter, and 9.5 mm bottom interior diameter. The glass vials were baked at 500 °C for 6 h before use. Most of the reagents were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Ultrapure water was prepared using a Milli-Q Integral (18.2 MΩ·cm, <5 ppb TOC). The starting solution was prepared with 600 μL of Gly solution (0.5 mol L−1) added to 0.5 mol boric acid, and the pH was adjusted with NaOH or HCl as shown in Supplementary Table 3. When the pH was not adjusted, the pH of the starting solutions was 6.5 and 5.3 in the absence and presence of boric acid, respectively. In we-dry cycle experiments, 600 μL of water was added to the vial and vortexed at 100 and 200 h.
The product was dissolved in 300 μL water and the resulting peptides were quantified using an ultra-performance liquid chromatography–tandem mass spectrometer (LC–MS/MS; Shimadzu LCMS-8040) using a VC-50 2D column (2.0 × 150 mm, 5 μm; Shodex) set to 50 °C. Eluent composition was changed linearly from 70% to 37% acetonitrile over 17 min at a flow rate of 0.25 mL min−1. Peptides were analyzed in the positive mode of electrospray ionization (ESI) with the nebulizer gas flow set to 2.5 L min−1, drying gas flow to 10 L min−1, desolvation temperature to 250 °C, and heat block temperature to 400 °C. Commercially available standard peptides of Gly2–5 and DKP were used for quantification. For samples in which long oligomers and polymers were formed, residues were present after dissolution with the first extract in 300 μL water. These residues were extracted into a second solution with 10–100 μL water or water/methanol, and the solutions were analyzed with a matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance mass spectrometer (FTICR-MS; solariX 9.4T; Bruker Daltonics, Billerica, MA, USA) or MALDI time of flight mass spectrometer (REFLEX III; Bruker Daltonics) with 2,5-dihydroxybenzoic acid as the matrix. Gly-B and Gly-BA formation was monitored with the negative mode of ESI with direct infusion of the diluted starting materials. The settings of the mass spectrometer were the same as for the LC–MS/MS analysis.
11B-NMR experiments were conducted using an 800 MHz NMR spectrometer (JNM-ECA800). Spectra were collected with 8–32 scans with a 2-s relaxation delay. The chemical shift values were reported based on a reference standard material (boric acid, pH 7, at 16.067 ppm). Samples were dissolved in D2O. FT-IR analysis of glycine and the mixture of glycine and boric acid was conducted with JASCO FT/IR-6300 with spectrum resolution of 4 cm−1 and 100 cumulative times.
Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Gilbert, W. Origin of life: the RNA world. Nature 319, 618 (1986).
Dreyfuss, G., Kim, V. N. & Kataoka, N. Messenger-RNA-binding proteins and the messages they carry. Nat. Rev. Mol. Cell Biol. 3, 195–205 (2002).
White, H. B. Coenzymes as fossils of an earlier metabolic state. J. Mol. Evol. 7, 101–104 (1976).
Poole, A. M., Jeffares, D. C. & Penny, D. The path from the RNA world. J. Mol. Evol. 46, 1–17 (1998).
Cech, T. R. Crawling out of the RNA world. Cell 136, 599–602 (2009).
Tagami, S., Attwater, J. & Holliger, P. Simple peptides derived from the ribosomal core potentiate RNA polymerase ribozyme function. Nat. Chem. 9, 325–332 (2017).
Frenkel-Pinter, M. et al. Mutually stabilizing interactions between proto-peptides and RNA. Nat. Commun. 11, 3137 (2020).
Müller, F. et al. A prebiotically plausible scenario of an RNA–peptide world. Nature 605, 279–284 (2022).
Glavin, D. P., Callahan, M. P., Dworkin, J. P. & Elsila, J. E. The effects of parent body processes on amino acids in carbonaceous chondrites. Meteorit. Planet. Sci. 45, 1948–1972 (2010).
Takeuchi, Y. et al. Impact-induced amino acid formation on Hadean Earth and Noachian Mars. Sci. Rep. 10, 1–7 (2020).
Lahav, N., White, D. & Chang, S. Peptide formation in the prebiotic era: thermal condensation of glycine in fluctuating clay environments. Science 201, 67–69 (1978).
Rodriguez-Garcia, M. et al. Formation of oligopeptides in high yield under simple programmable conditions. Nat. Commun. 6, 8385 (2015).
Campbell, T. D. et al. Prebiotic condensation through wet–dry cycling regulated by deliquescence. Nat. Commun. 10, 4508 (2019).
Sakata, K., Kitadai, N. & Yokoyama, T. Effects of pH and temperature on dimerization rate of glycine: evaluation of favorable environmental conditions for chemical evolution of life. Geochim. Cosmochim. Acta 74, 6841–6851 (2010).
Imai, E., Honda, H., Hatori, K., Brack, A. & Matsuno, K. Elongation of oligopeptides in a simulated submarine hydrothermal system. Science 283, 831–833 (1999).
Ohara, S., Kakegawa, T. & Nakazawa, H. Pressure effects on the abiotic polymerization of glycine. Orig. Life Evol. Biosph. 37, 215–223 (2007).
Canavelli, P., Islam, S. & Powner, M. W. Peptide ligation by chemoselective aminonitrile coupling in water. Nature 571, 546–549 (2019).
Ricardo, A., Carrigan, M. A., Olcott, A. N. & Benner, S. A. Borate minerals stabilize ribose. Science 303, 196 (2004).
Scorei, R. & Cimpoiasu, V. M. Boron enhances the thermostability of carbohydrates. Orig. Life Evol. Biosph. 36, 1–11 (2006).
Furukawa, Y., Horiuchi, M. & Kakegawa, T. Selective stabilization of ribose by borate. Orig. Life Evol. Biosph. 43, 353–361 (2013).
Furukawa, Y., Kim, H. J., Hutter, D. & Benner, S. A. Abiotic regioselective phosphorylation of adenosine with borate in formamide. Astrobiology 15, 259–267 (2015).
Kim, H. J. et al. Evaporite borate-containing mineral ensembles make phosphate available and regiospecifically phosphorylate ribonucleosides: borate as a multifaceted problem solver in prebiotic chemistry. Ang. Chem. Int. Ed. 55, 15816–15820 (2016).
Becker, S. et al. Unified prebiotically plausible synthesis of pyrimidine and purine RNA ribonucleotides. Science 366, 76–82 (2019).
Franco, A., Ascenso, J. R., Ilharco, L. & Silva, J. A. L. D. Synthesis of ribonucleotides from the corresponding ribonucleosides under plausible prebiotic conditions within self-assembled supramolecular structures. N. J. Chem. 44, 2206–2209 (2020).
Hirakawa, Y., Kakegawa, T. & Furukawa, Y. Borate-guided phosphorylation for prebiotic nucleotide synthesis. Sci. Rep. 12, 11828 (2022).
Furukawa, Y. & Kakegawa, T. Borate and the origin of RNA: a model for the precursors to life. Elements 13, 261–265 (2017).
Benoit, P. Is boric acid the missing link in prebiotic chemistry? in First Steps in the Origin of Life in the Universe: Proceedings of the Sixth Trieste Conference on Chemical Evolution Trieste, 103–106 (2001).
Ishihara, K., Ohara, S. & Yamamoto, H. 3,4,5-Trifluorobenzeneboronic acid as an extremely active amidation catalyst. J. Org. Chem. 61, 4196–4197 (1996).
Sabatini, M. T., Boulton, L. T. & Sheppard, T. D. Borate esters: Simple catalysts for the sustainable synthesis of complex amides. Sci. Adv. 3, e170102 (2017).
Tang, P. Boric acid catalyzed amide formation from carboxylic acids and amines: N-Benzyl-4-phenylbutyramide. Org. Synth. 81, 262–272 (2005).
Van Duin, M., Peters, J. A., Kieboom, A. P. G. & Van Bekkum, H. Studies on borate esters 1: the pH dependence of the stability of esters of boric acid and borate in aqueous medium as studied by 11B NMR. Tetrahedron 40, 2901–2911 (1984).
Gündüz, M. K. et al. Therapeutic effects of newly synthesized boron compounds (BGM and BGD) on hepatocellular carcinoma. Biol. Trace Elem. Res. 200, 134–146 (2022).
Kelley, D. et al. An off-axis hydrothermal vent field near the Mid-Atlantic Ridge at 30° N. Nature 412, 145–149 (2001).
Von Damm, K. L. Seafloor hydrothermal activity: black smoker chemistry and chimneys. Annu. Rev. Earth Planet. Sci. 18, 173–204 (1990).
Shibuya, T. et al. Hydrogen-rich hydrothermal environments in the Hadean ocean inferred from serpentinization of komatiites at 300 °C and 500 bar. Prog. Earth Planet. Sci. 2, 46 (2015).
Jerome, C. A., Kim, H. J., Mojzsis, S. J., Benner, S. A. & Biondi, E. Catalytic synthesis of polyribonucleic acid on prebiotic rock glasses. Astrobiology 22, 629–636 (2022).
Halevy, I. & Bachan, A. The geologic history of seawater pH. Science 355, 1069–1071 (2017).
Wilde, S. A., Valley, J. W., Peck, W. H. & Graham, C. M. Evidence from detrital zircons for the existence of continental crust. Nature 409, 175–178 (2001).
Grew, E. S. et al. Boron isotopes in tourmaline from the ca. 3.7-3.8Ga Isua supracrustal belt, Greenland: sources for boron in Eoarchean continental crust and seawater. Geochim. Cosmochim. Acta 163, 156–177 (2015).
Wang, F. et al. A systematic survey of mini-proteins in bacteria and archaea. PLoS One 3, e4027 (2008).
Su, M., Ling, Y., Yu, J., Wu, J. & Xiao, J. Small proteins: untapped area of potential biological importance. Front. Genet. 4, 286 (2013).
Serganov, A., Huang, L. & Patel, D. Structural insights into amino acid binding and gene control by a lysine riboswitch. Nature 455, 1263–1267 (2008).
Yagi, S. et al. Seven amino acid types suffice to create the core fold of RNA polymerase. J. Am. Chem. Soc. 143, 15998–16006 (2021).
Li, P., Holliger, P. & Tagami, S. Hydrophobic-cationic peptides modulate RNA polymerase ribozyme activity by accretion. Nat. Commun. 13, 3050 (2022).
Acknowledgements
The authors acknowledge Hiroyuki Momma, Shinichiro Yoshida, and Daisuke Unabara for the acquisition of FTICR-MS, 11B-NMR, and TOF-MS data. Y.F. and T.K. acknowledge The Japan Society for the Promotion of Science KAKENHI 18H03729 for T.K. and 22H00165 for Y.F.
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Y.F. conceived the study and managed the research. Y.F. and K.S. developed experimental and analytical methods. Y.S. and Y.F. performed the experiments and prepared the low data. T.K. and Y.F. contributed funding acquisition. Y.F. and Y.S. write the initial manuscript. All authors revised and improved the manuscript.
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Sumie, Y., Sato, K., Kakegawa, T. et al. Boron-assisted abiotic polypeptide synthesis. Commun Chem 6, 89 (2023). https://doi.org/10.1038/s42004-023-00885-7
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DOI: https://doi.org/10.1038/s42004-023-00885-7
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