Polymers of ribonucleotides (RNAs) are considered to store genetic information and promote biocatalytic reactions for the proto life on chemical evolution. Abiotic synthesis of ribonucleotide was successful in past experiments; nucleoside synthesis occurred first, followed by phosphorylation. These abiotic syntheses are far from biotic reactions and have difficulties as a prebiotic reaction in reacting chemicals in a specific order and purifying intermediates from other molecules in multi-steps of reactions. Another reaction, ribose phosphorylation followed by nucleobase synthesis or nucleobase addition, is close to the biotic reactions of nucleotide synthesis. However, the synthesis of ribose 5′-phosphate under prebiotically plausible conditions remains unclear. Here, we report a high-yield regioselective one-pot synthesis of ribose 5′-phosphate from an aqueous solution containing ribose, phosphate, urea, and borate by simple thermal evaporation. Of note, phosphorylation of ribose before the nucleoside formation differs from the traditional prebiotic nucleotide syntheses and is also consistent with biological nucleotide synthesis. Phosphorylation occurred to the greatest extent in ribose compared to other aldopentoses, only in the presence of borate. Borate is known to improve the stability of ribose preferentially. Geological evidence suggests the presence of borate-rich settings on the early Earth. Therefore, borate-rich evaporitic environments could have facilitated preferential synthesis of ribonucleotide coupled with enhanced stability of ribose on the early Earth.
Abiotic formation of functional polymers with catalytic capabilities and information-carrying capacities would have been essential for the emergence of primitive life. The discovery of the catalytic RNAs supports that RNA might have served these functions in the primordial life, as proposed by the RNA world hypothesis1,2. Abiotic formation of RNA monomers (i.e., ribonucleotides) on the prebiotic Earth is considered as an essential step in the prebiotic RNA synthesis. However, the process of the abiotic formation of ribonucleotides on the Hadean Earth needs further elucidation.
Nucleotides have been chemically synthesized via the formation of nucleosides and subsequent phosphorylation of these molecules3,4,5,6,7,8,9,10,11,12. In abiotic nucleoside syntheses, condensation between ribose 1 and nucleobase results in the formation of purine nucleosides in low yields and pyrimidine nucleosides in no detectable yields3. A high yielding nucleoside synthesis needs step-by-step reactions of small reactive molecules with separation and purification of the intermediate chemical compounds4,5,6; the geological settings/events that would have permitted such step-wise synthesis remain unclear. On the other hand, phosphorylation of nucleosides occurs by simple drying of nucleoside and phosphate 2 with catalysts such as urea 37,8,9,10,11,12. Ribose 1 can form via a simple condensation of formaldehyde, which can, in turn, be formed by a photochemical reaction between CO2 and H2O13,14. These hint at a possibility of phosphorylation of ribose 1 before the nucleoside formation on Hadean Earth. Further, phosphorylation of sugar before the nucleoside formation is seen in nucleotide biosynthesis in extant life. Phosphorylation of sugar is also an essential reaction related to metabolism in all living cells. Given this, we investigated the phosphorylation of ribose 1 under prebiotically plausible condition.
The sugar phosphate backbone of RNA consists of phosphate 2 and ribose 1 with phosphodiester linkages at 5′- and 3′-hydroxyl groups on ribose 1. Simple heating of ribose 1 and phosphate 2 with cyanogen 4 selectively yields ribose 1′-phosphate 515. Further, the reaction of ribose 1 with amidotriphosphate selectively yields ribose 1′,2′-cyclic phosphate16. The synthesis of ribose 1′-phosphate 5 was also demonstrated by artificial electrospray-ionized microdroplets using ribose 1 and phosphate 217. The synthesis of ribose 5′-phosphate 6 has only been achieved by similar artificial electrospray-ionized microdroplets using ribose 1 and pyrophosphate18. Nonetheless, the availability of electrospray-ionized microdroplets on the Hadean Earth is questionable. Thus, the synthesis of biologically relevant ribose-phosphates (i.e., ribose 5′- and 3′-phosphate) under plausible prebiotic conditions remains elusive.
Urea 3, a simple amide molecule, has been used as an efficient abiotic catalyst for the phosphorylation of nucleosides8,19. In addition, previous research reported that urea 3 could be a nucleobase precursor on the prebiotic Earth20,21. Urea 3 is formed via hydrolysis of cyanamide that can form from ammonia and cyanide19. Both ammonia and cyanide can be formed by impact-induced reactions22. These reactions would have been common on the Hadean Earth when the impacts of large meteorites and asteroids were more frequent than today22,23. Thus, it is reasonable to assume urea as a prebiotically available molecule on the early Earth. However, the effects of urea 3 on the phosphorylation of sugars have not been investigated. Therefore, we investigated the phosphorylation reaction of aldopentoses in the presence of urea 3.
Sugars are readily decomposed by heating. In particular, ribose is the least stable aldopentose24. Previous studies have found that several oxyanions can improve the stability of sugars13,25,26. In particular, borate 7 can strongly stabilize ribose 1 compared to other aldopentoses27,28. This indicates that borate-rich environments could have been advantageous for nucleotide formation as ribose 1 could have accumulated in such environments. Furthermore, borate 7 is known to control the phosphorylation site of nucleosides29. The effects of borate 7 on other steps involved in prebiotically plausible nucleotide synthesis have been reported in many previous studies6,30,31. Therefore, we investigated the effects of borate 7 on the phosphorylation of ribose 1 and other aldopentoses under evaporation.
Here, we show the regioselective synthesis of ribose 5′-phosphate 6 from ribose 1 and dissolved phosphate 2 in the presence of urea 3 and borate 7 under simple drying conditions (Fig. 1). A neutral aqueous solution containing d-ribose, disodium monophosphate, urea, and boric acid (pH 8) was dried by heating at 80 °C for 24 h. The precipitates were hydrolyzed in acidic water (pH ~ 1) at 90 °C for 1 h and analyzed using high-performance liquid chromatography-tandem mass spectrometry (LC–MS/MS). The formation of significant amount of ribose 5′-phosphate 6 (i.e., 22 mol% yield on average; n = 3; 1σ = ± 2.5) in the reactions containing boric acid was confirmed by its LC retention time, MS/MS fragmentation pattern, and 31P-NMR (Fig. 2c–e, Figs. S1, S2, and S3). In the reaction with no boric acid, a small amount of ribose 5′-phosphate 6 (i.e., 4 mol% yields) was detected (Fig. 2c). A small amount of ribose 5′-phosphate 6 (i.e., 7 mol% yields) was also detected in the product formed with boric acid before acid hydrolysis. Any ribose phosphate was not formed in the acid condition from ribose and phosphate (Fig. S4). The yield of ribose 5′-phosphate 6 in the reaction without boric acid before acid hydrolysis was < 0.1 mol% (Fig. 2a). In the mass chromatogram corresponding to the molecular mass of ribosylurea phosphate 8, two significant peaks, indicating the presence of ribosylurea phosphate 8, were detected only in the reaction containing boric acid before acid hydrolysis (Figs. 2b and S5). Amines tend to react with aldehydes to form imines. Thus, we envisage that 1′-aldehyde of ribose 1 reacted with urea 3 to form ribosylurea 932.
These results indicate that ribose 1 was regioselectively phosphorylated at the 5′-hydroxyl group in the presence of borate 7. The residual fraction of ribose 1 was found to be 19 mol% in the presence of borate 7 and 3 mol% in the absence of borate 7. This shows that borate improved the stability of ribose 1 in the reaction (Fig. S6). Borate 7 forms a complex with a diol facing the same direction. In the case of ribose 1, complex formation with its 1′- and 2′-hydroxyl groups improves the stability of ribose 1 by fixing its form in furanose structure33. In the present reaction, urea 3 reacted with 1′-hydroxyl of ribose 132. Thus, borate 7 might have formed a complex with ribose 1 at its 2′, 3′-diol. The complex formation fixed ribose 1 in the furanose form and improved its stability. This led to the high yielding regioselective phosphorylation of ribose 1 at its 5′-hydroxyl (Fig. 3). Phosphorylation at 5′-hydroxyl limits the form of ribose exclusively in furanose, whereas the form of ribose in 2′-phosphate, 3′-phosphate, and 2′,3′-cyclic phosphate can be both furanose and pyranose.
This one-pot synthesis of ribose 5′-phosphate 6 is a simpler reaction than multistep nucleoside syntheses and indicates that ribose phosphorylation could have occurred before the nucleoside formation on the Hadean Earth, in particular, in borate-rich environments. A previous study showed the formation of α-cytidine nucleotide from ribose 5′-phosphate using cyanamide and cyanoacetylene34. Another previous study reported that a photochemical reaction can convert the α-nucleotide to canonical β-nucleotide4. Thus, nucleotide formation from ribose 5′-phosphate is possible using small reactive molecules via a photochemical reaction, although the net yield is unclear4,34. Thus, the ribose phosphate synthesis shown in this study opens a new route for prebiotic ribonucleotide synthesis. This route is geochemically more plausible than traditional prebiotic nucleotide syntheses and consistent with extant biological nucleotide synthesis.
We further evaluated the phosphorylation of other aldopentoses (i.e., arabinose 10, xylose 11, and lyxose 12; Fig. S7) in the presence of borate 7. The phosphorylated products of aldopentoses other than ribose 1 showed multiple peaks in LC–MS/MS chromatograms, indicating that the phosphorylation occurred at different hydroxyls on each aldopentose (Fig. 4). The extent of phosphorylation was evaluated based on the area under the peak of phosphorylated aldopentoses, assuming that the ionization efficiency of these compounds is similar to that of ribose 5′-phosphate 6 (Fig. S8). Ribose 1 was selectively phosphorylated at 5′-hydroxyl group with the highest yield of 22 mol%, whereas other pentoses were phosphorylated at varied hydroxyl positions with total yields of 11, 8, and 19 mol% for phosphorylated arabinose 10, xylose 11, and lyxose 12, respectively (Fig. 4). Selectivity of phosphorylation was not apparent in the reactions carried out without borate 7 (Fig. S9). Therefore, the borate-guided phosphorylation of pentoses shows the preferential synthesis of ribose 5′-phosphate 6.
The residual fraction of each aldopentose was 19, 41, 22, and 16 mol% for ribose 1, arabinose 10, xylose 11, and lyxose 12, respectively, in the presence of borate 7 (Fig S7). Compared to other aldopentoses, the rate of phosphorylation based on the residual amounts of pentoses and their phosphorylated products was found to be higher for ribose 1 and lyxose 12 (Figs. 4, S7, S9, and S10). Ribosylurea 9 and lyxosylurea 13 tend to react with borate 7 at their 2′, 3′-diol and get fixed in their respective furanose forms33 because borate 7 forms a more stable complex with diols than with single hydroxyl groups33. The remaining 5′-hydroxyl group can then react with phosphate 2 (Fig. S11). On the other hand, arabinosylurea 14 and xylosylurea 15 might remain in their chain forms when these molecules are combined with borate 7 due to different directions of hydroxyls on these molecules, as compared to those on ribose 1 and lyxose 12 (Fig. S11). Because the chain form of ureido-pentoses can also combine with borate 7 at their 3′, 4′-diol and 4′, 5′-diol positions in addition to 2′, 3′-diol position, the phosphorylation can get partially inhibited by these borate-diol complexes. These differences in the forms of complexes with borate 7 might underly the different phosphorylation efficiencies observed for varied pentoses as well as the highest phosphorylation observed for ribose 1 (Fig. S11).
Tourmaline, a boron-rich mineral, has been found in > 3.7-billion-year-old metasediments in Isua, Greenland35,36. Further, the likely presence of early evaporitic environments has been reported37. These early Archean geological conditions presumably extended back to the Hadean Earth. Borate 7 has been thought to have been present in the Hadean oceans as well; this might have led to borate 7 accumulation in evaporitic environments on the Hadean Earth38. Ribose 1 might have preferentially accumulated over other aldopentoses in such environments as borate 7 is known to contribute to the stabilization of ribose 113,38. A higher concentration of carbonate than present is expected in the Hadean ocean, covered by a CO2-rich atmosphere. In such an ocean, higher phosphate 2 concentrations than today are expected due to the consumption of Ca2+, a cation to form Ca2+-phosphate, as Ca2+-carbonate39,40,41,42. The evaporation processes could have also accumulated dissolved phosphate 2 and urea 3 in the same place as borate 7 and ribose 1 and further induced dehydration reaction leading to the phosphorylation of ribose 1. The present results indicate that the borate-rich evaporitic environment on prebiotic Earth could have enabled the preferential synthesis of ribose 5′-phosphate 6 before the formation of nucleosides.
Previous research reported nucleotide formation from ribose 5′-phosphate 6 using small reactive molecules or amino acids35,43, although the nucleotide synthesis through ribose 5′-phosphate 6 needs further investigation. Furthermore, many previous papers reported nucleoside formation from ribose under prebiotically plausible conditions3,6,30,44,45. The literature indicates that nucleotide formation using ribose 5′-phosphate 6 could be possible on the prebiotic Earth. Therefore, the one-pot synthesis of ribose 5′-phosphate 6 opens the renounced abiotic route of ribonucleotide formation. This route is more geochemically plausible and analogous to its biosynthesis (Fig. 1). This may provide a geochemical explanation regarding how ribose 1, the least stable aldopentose24, became the selected sugar in RNA.
Reagent grade d-ribose, urea, boric acid, sulfuric acid, sodium hydroxide, disodium phosphate, and dipotassium phosphate were obtained from Wako Pure Chemical Industries (Osaka, Japan). Regent grade d-ribose 5′-phosphate disodium salt dihydrate, adenosine 2′-monophosphate, and adenosine 3′-monophosphate were obtained from Sigma-Aldrich and used as standards. All chemicals were used without further purification. LC–MS grade ammonium formate (Sigma-Aldrich) and acetonitrile (Merck) were used for LC–MS/MS analyses. Water was purified using Milli-Q Integral (18.2 MΩ·cm and < 3 ppb TOC).
Preparative synthesis of ribose-phosphate and ribosylurea
LC–MS/MS standards for ribose 2′-phosphate and ribose 3′-phosphate were prepared by heating 20 mM of 2′-AMP and 3′-AMP, respectively, at 90 °C for 1 h in 2 wt% sulfuric acid solution to hydrolyze N-glycosidic bond between ribose and urea. LC–MS/MS standard of ribosylurea phosphate was prepared by heating 20 mM of ribose 5′-phosphate and 80 mM urea at 60 °C for 24 h.
The phosphorylation experiments were conducted in Eppendorf tubes. The 20 µL reaction mixture contained 20 mM ribose, 800 mM urea, 40 mM boric acid, and 160 mM disodium phosphate in water. The starting materials were heated at 80 °C for 24 h with the lids of the tubes kept open. After heating, the residues were resuspended in 200 µL of water. Borate was removed from the product by adding 4 µL sulfuric acid (95%) to the resuspended sample solution before LC–MS/MS analysis. For investigating the acid hydrolyzed product, a sulfuric acid solution containing the experimental residue was heated at 90 °C for 1 h with the lid closed. The hydrolysis would have progressed slowly in the prebiotic ocean, where pH was close to neutral, and the temperature was lower than the acid treatment, although the rate of reaction should be lower.
The control experiment in acidic conditions was conducted in Eppendorf tubes. 20 µL of reaction mixture containing 20 mM ribose, 800 mM urea, 40 mM boric acid, and 160 mM disodium phosphate in acidic water adjusted pH ~ 1 with sulfuric acid was dried down at 80 °C. The experimental and analytical procedures were the same as other experiments. For investigating the effect of acid treatment on phosphorylation, the starting material before the experiment was diluted to tenfold with pure water, adjusted at pH ~ 1 with sulfuric acid, and heated at 90˚C for 1 h.
The separation and detection of the products were conducted using Shimadzu LCMS 8040 (Kyoto; Japan) with the hydrophilic interaction chromatography mode using HILICpak VT-50 2D column (5 μm, 2.0 mm ID, 150 mm length; Shodex). The sample was eluted with isocratic elution with an aqueous solution containing 80% 25 mM ammonium formate and 20% acetonitrile at a total flow rate of 0.2 mL/min at 60 °C. Mass spectrometry was conducted in negative mode with desolvation, source, and heat block temperatures set at 250 °C, 120 °C, and 400 °C, respectively.
31P-NMR spectra were acquired with Bruker AVANCE III 500 spectrometer. High concentration samples necessary for the NMR analysis were obtained by the following method. The 20 µL aqueous solution containing 200 mM ribose, 4 M urea, 400 mM boric acid, and 1.6 M dipotassium phosphate was heated at 80 °C for 24 h with the lids of the tube kept open. Dipotassium phosphate was used instead of disodium phosphate due to its better solubility. After heating, the residues were resuspended in 200 µL of water, added by 4 µL sulfuric acid (95%), and heated at 90 °C for 1 h. Before the 31P-NMR analysis, pH was adjusted to about 8 with sodium hydroxide solution.
All data are available in the main text and supplementary information.
Walter, G. The RNA world. Nature 319, 618 (1986).
Joyce, G. F. RNA evolution and the origins of life. Nature 338, 217–224 (1989).
Fuller, W. D., Sanchez, R. A. & Orgel, L. E. Studies in prebiotic synthesis. VII. J. Mol. Evol. 1, 249–257 (1972).
Powner, M. W., Gerland, B. & Sutherland, J. D. Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible conditions. Nature 459, 239–242 (2009).
Stairs, S. et al. Divergent prebiotic synthesis of pyrimidine and 8-oxo-purine ribonucleotides. Nat. Commun. 8, 15270 (2017).
Becker, S. et al. Unified prebiotically plausible synthesis of pyrimidine and purine RNA ribonucleotides. Science 366, 76–82 (2019).
Lohrmann, R. & Orgel, L. E. Prebiotic synthesis: Phosphorylation in aqueous solution. Science 161, 64–66 (1968).
Lohrmann, R. & Orgel, L. E. Urea-inorganic phosphate mixtures as prebiotic phosphorylating agents. Science 171, 1–5 (1968).
Shoffstall, A. M. Prebiotic phosphorylation in formamide of nucleosides. Orig. Life Evol. Biosph. 7, 399–412 (1976).
Schwartz, A. & Ponnamperuma, C. Phosphorylation of adenosine with liner polyphosphate salts in aqueous solution. Nature 218, 443 (1968).
Gull, M. et al. Nucleoside phosphorylation by the mineral schreibersite. Sci. Rep. 5, 2–7 (2015).
Burcar, B. et al. Darwin’s warm little pond: A one-pot reaction for prebiotic phosphorylation and the mobilization of phosphate from minerals in a urea-based solvent. Angew. Chem. Int. 55, 13249–13253 (2016).
Ricardo, A., Carrigan, M. A., Olcott, A. N. & Benner, S. A. Borate minerals stabilize ribose. Science 303, 196 (2004).
Pinto, J. P., Gladstone, G. R. & Yung, Y. L. Photochemical production of formaldehyde in Earth’s primitive atmosphere. Science 210, 183–185 (1980).
Halmann, M., Sanchez, R. A. & Orgel, L. E. Phosphorylation of d-ribose in aqueous solution. J. Org. Chem. 34, 3702–3703 (1969).
Krishnamurthy, R., Guntha, S. & Eschenmoser, A. Regioselective α-phosphorylation of aldoses in aqueous solution. Angew. Chem. Int. 39, 2281–2285 (2000).
Nam, I., Lee, J. K., Nam, H. G. & Zare, R. N. Abiotic production of sugar phosphates and uridine ribonucleoside in aqueous microdroplets. Proc. Natl. Acad. Sci. U.S.A. 114, 12396–12400 (2017).
Hu, J., Lei, W., Wang, J., Chen, H. Y. & Xu, J. J. Regioselective 5′-position phosphorylation of ribose and ribonucleosides: phosphate transfer in the activated pyrophosphate complex in the gas phase. Chem. Comm. 55, 310–313 (2019).
Hud, N. V. Searching for lost nucleotides of the pre-RNA World with a self-refining model of early Earth. Nat. Commun. 9, 1–4 (2018).
Robertoson, M. P. & Miller, S. L. An efficient prebiotic synthesis of cytosine and uracil. Nature 375, 772–774 (1995).
Shapiro, R. Prebiotic cytosine synthesis: A critical analysis and implications for the origin of life. Proc. Natl. Acad. Sci. USA 96, 4396–4401 (1999).
Furukawa, Y., Samejima, T., Nakazawa, H. & Kakegawa, T. Experimental investigation of reduced volatile formation by high-temperature interactions among meteorite constituent materials, water, and nitrogen. Icarus 231, 77–82 (2014).
Benner, S. A., Bell, E. A., Biondi, E., Brasser, R., Carell, T., Kim, H. J., Mojzsis, S. J., Omran, A., Pasek, M. A. & Trail, D. When did life likely emerge on earth in an RNA-first process? ChemSystemsChem (2019).
Larralde, R., Robertscn, M. P. & Miller, S. L. Rates of decomposition of ribose and other sugars: Implications for chemical evolution. Proc. Natl. Acad. Sci. USA 92, 8158–8160 (1995).
Lambert, J. B., Gurusamy-Thangavelu, S. A. & Ma, K. The silicate-mediated formose reaction: Bottom-up synthesis of sugar silicates. Science 327, 984–986 (2010).
Kim, H. J. et al. Synthesis of carbohydrates in mineral-guided prebiotic cycles. J. Am. Chem. Soc. 133, 9457–9468 (2011).
Šponer, J. E., Sumpter, B. G., Leszczynski, J., Šponer, J. & Fuentes-Cabrera, M. Theoretical study on the factors controlling the stability of the borate complexes of ribose, arabinose, lyxose, and xylose. Chem. Eur. J. 14, 9990–9998 (2008).
Furukawa, Y., Horiuchi, M. & Kakegawa, T. Selective stabilization of ribose by borate. Orig. Life Evol. Biosph. 43, 353–361 (2013).
Furukawa, Y., Kim, H. J., Hutter, D. & Benner, S. A. Abiotic regioselective phosphorylation of adenosine with borate in formamide. Astrobiology 15, 259–267 (2015).
Becker, S. et al. A high-yielding, strictly regioselective prebiotic purine nucleoside formation pathway. Science 352, 833–836 (2016).
Kim, H. J. et al. Evaporite borate-containing mineral ensembles make phosphate available and regiospecifically phosphorylate ribonucleosides: Borate as a multifaceted problem solver in prebiotic chemistry. Angew. Chem. Int. 55, 15816–15820 (2016).
Okamura, H. et al. Proto-Urea-RNA (Wöhler RNA) containing unusually stable urea nucleosides. Angew. Chem. Int. 58, 18691–18696 (2019).
Chapelle, S. & Verchere, J.-F. A 11B and 13C NMR determination of the structures of borate complexes of pentoses and related sugars. Tetrahedron 44, 4469–4482 (1988).
Sanchez, R. A. & Orgel, L. E. Studies in prebiotic synthesis. V. Synthesis and photoanomerization of pyrimidine nucleosides. J. Mol. Biol. 47, 531–543 (1970).
Appel, P. W. U. Tourmalinites in the 3800 Ma old Isua supracrustal belt, West Greenland. Precambrian Res. 72, 227–234 (1995).
Grew, E. S. et al. Boron isotopes in tourmaline from the ca. 3.7–3.8 Ga Isua supracrustal belt, Greenland: Sources for boron in Eoarchean continental crust and seawater. Geochim. Cosmochim. Acta 163, 156–177 (2015).
Nabhan, S., Luber, T., Scheffler, F. & Heubeck, C. Climatic and geochemical implications of Archean pedogenic gypsum in the Moodies Group (~3.2Ga), Barberton Greenstone Belt, South Africa. Precam. Res. 275, 119–134 (2016).
Furukawa, Y. & Kakegawa, T. Borate and the origin of RNA: A model for the precursors to life. Elements 13, 261–265 (2017).
Kakegawa, T., Noda, M. & Nannri, H. Geochemical cycles of bio-essential elements on the early earth and their relationships to origin of life. Resour. Geol. 52, 83–89 (2002).
Toner, J. D. & Catling, D. C. A carbonate-rich lake solution to the phosphate problem of the origin of life. Proc. Natl. Acad. Sci. USA 117, 883–888 (2020).
Kasting, J. F. Earth’s early atmosphere. Science 259, 920–926 (1993).
Zahnle, K., Schaefer, L. & Fegley, B. Earth’s earliest atmospheres. Cold Spring Harb. Perspect. Biol. 2, 1–17 (2010).
Suárez-Marina, I. et al. Integrated synthesis of nucleotide and nucleosides influenced by amino acids. Commun. Chem. 2, 1–8 (2019).
Kim, H. J. & Benner, S. A. Prebiotic stereoselective synthesis of purine and noncanonical pyrimidine nucleotide from nucleobases and phosphorylated carbohydrates. Proc. Natl. Acad. Sci. USA 114, 11315–11320 (2017).
Kim, H. J. & Kim, J. A prebiotic synthesis of canonical pyrimidine and purine ribonucleotides. Astrobiology 19, 669–674 (2019).
This work has been supported by the Japan Society for the Promotion of Science to Y. H. (21J22596), Y. F. (22H00165), and T. K. (15H02144 and 18H03729), the Tohoku University FRIS research program for T. K. and Y. F., and WISE Program for Sustainability in the Dynamic Earth to Y. H. for financial support. We thank Daisuke Unabara for 31P-NMR data acquisition.
The authors declare no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Hirakawa, Y., Kakegawa, T. & Furukawa, Y. Borate-guided ribose phosphorylation for prebiotic nucleotide synthesis. Sci Rep 12, 11828 (2022). https://doi.org/10.1038/s41598-022-15753-y