Abstract
After activation, some invariant natural killer T (iNKT) cells are differentiated into Klrg1+ long-lived effector NKT1 cells. However, the regulation from the effector phase to the memory phase has not been elucidated. Zeb2 is a zinc finger E homeobox-binding transcription factor and is expressed in a variety of immune cells, but its function in iNKT cell differentiation remains also unknown. Here, we show that Zeb2 is dispensable for development of iNKT cells in the thymus and their maintenance in steady state peripheral tissues. After ligand stimulation, Zeb2 plays essential roles in the differentiation to and maintenance of Klrg1+ Cx3cr1+GzmA+ iNKT cell population derived from the NKT1 subset. Our results including single-cell-RNA-seq analysis indicate that Zeb2 regulates Klrg1+ long-lived iNKT cell differentiation by preventing apoptosis. Collectively, this study reveals the crucial transcriptional regulation by Zeb2 in establishment of the memory iNKT phase through driving differentiation of Klrg1+ Cx3cr1+GzmA+ iNKT population.
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Introduction
Invariant Natural killer T (iNKT) cells are a unique type of innate T lymphocytes that stimulate or regulate subsequent immune responses. iNKT cells recognize glycolipids presented by the MHC class I-like molecule CD1d1,2. The rapid response of iNKT cells to their cognate antigens is characteristic of the innate immune response and allows the release of polarizing cytokines (IFN-γ and/or IL-4)3. These characteristics are shared with other effector lymphocytes, such as conventional effector or memory CD4+ and CD8+T cells, NK cells, mucosal-associated invariant T cells, and γδT cells4,5. In commitment to the iNKT cell lineage in the thymus, iNKT cell precursors are positively selected by double-positive thymocytes, which express CD1d-glycolipid complexes6. Because iNKT cells have divergent epigenetic expressions, transcription profiles, and gene expression programs, iNKT cells were separated into three subsets, NKT1, NKT2, and NKT17 cells, in which the key transcription factors, T-bet, Gata3, and Rorγt respectively, contribute to NKT1, NKT2, and NKT17 development7,8,9. Numerous genes were differentially expressed and regulated in each iNKT cell subset.
After egress from the thymus, these iNKT-cell subsets undergo further differentiation in the periphery. In C57BL/6 mice, individual iNKT cell subsets have a unique expression pattern of homing molecules8, which may correlate with their difference in tissue distribution and function of the three NKT subsets10. In addition, several groups have analyzed the molecular details of iNKT cell differentiation by genome-wide analysis of gene expression, showing that most do not recirculate8,11,12. A recent study reported that the impact of tissue localization and homeostasis in peripheral organs rather than the thymus was determined by both iNKT subsets and activation status, which were proved by transcriptome and chromatin landscapes of iNKT cell studies13. Although iNKT cells express effector and/or memory T-cell markers, such as CD44, and CD69 in the steady state, how the phenotype and function of iNKT cells after activation can be controlled in peripheral tissues, remain to be determined.
Innate lymphocytes, which mount rapid effector responses, are thought to be short-lived. However, NK cells14 and γδT cells15 with memory type characteristics have previously been identified. We previously reported that Klrg1+ iNKT1 cells elicited after administration of α-GalCer-loaded DCs (DC/Gal) or -CD1d-expressing NIH3T3 cells are long-lived effector cells16. Klrg1+ iNKT cells, expressing the NKT1-like cytokine IFN-γ and cytotoxic molecules (FasL and granzyme A/B), persisted for several months in the lung and maintained long-term anti-tumor function. They displayed a robust secondary response to cognate antigen16. Analyses of CDR3β by RNA deep sequencing the iNKT cells in DC/Gal pre-injected mice demonstrated that some particular Klrg1+iNKT-cell clones accumulated, suggesting the selection of a certain TCR repertoire by an antigen. These findings demonstrated the effector memory-like Klrg1+ iNKT cells. These cells develop in a process dependent on the transient expression of Eomes peripherally17. We also demonstrated that Eomes plays an important role in the development of NKT1 cells in the thymus17; furthermore, other studies also characterized the Klrg1+ iNKT cells. Murray et al. also confirmed that α-GalCer treatment induced effector-like NKT cells in the spleen that expressed Klrg1 and Cx3cr1, which were derived from the NKT1 subset13. Plasit et al. also reported infiltration of Klrg1+ iNKT cells at the tumor site and draining lymph nodes elicited by intratumoral injection of CpG and α-GalCer, which enhanced the abscopal effect in the antitumor effect of CD8 T cells18. However, it remains unknown which specific molecules play a key role in the differentiation of Klrg1+ iNKT cells in the periphery without affecting the thymus.
Zinc finger E-box-binding homeobox 2 (Zeb2) is a transcription factor that acts as a transcriptional repressor by cooperating with activated SMAD proteins and by recruiting of either the corepressor C-terminal binding protein or histone deacetylase complexes, such as nucleosome remodeling and deacetylase19. Initially, Zeb2 mediates the epithelial-to-mesenchymal transition through repression of epithelial genes. Recently, the role of Zeb2 in the differentiation and function of NK and T cells, as well as myeloid cells, DCs, and macrophages, was revealed20,21,22,23,24,25,26,27. However, the importance of Zeb2 in iNKT cells has not yet been reported; but Zeb2 has been reported to cooperate with Klrg1 in T and NK cells. In this study, we examined whether Zeb2 is involved in the development of iNKT cells in the thymus or periphery using T-cell-specific Zeb2-conditional knockout mice (Zeb2-cKO).
Results
Klrg1+ iNKT cells elicited by DC/Gal express Zeb2
Klrg1+ iNKT cells increased after the administration of DC/Gal16. We analyzed the pattern of gene expression, especially transcription factors of lung iNKT cells from naïve and DC/Gal-immunized mice using RNAseq data previously reported17 (Supplementary Fig. 1a). Fold change (DC/Gal versus naïve) showed the highest upregulation of Zeb2 (Fig. 1a). After sorting Klrg1+ iNKT cells on day 7, we confirmed that Zeb2 transcripts were highly expressed in Klrg1+ iNKT cells (Fig. 1b). In T and NK cells, it was previously shown that Zeb2 expression is dependent on T-bet in both Th1 and NK cells20,21,22. To determine whether the T-bet also functions in iNKT cells for Zeb2 induction, when T-bet KO mice were administered with DC/Gal, a lower frequency of Klrg1+ iNKT cells was detected (Supplementary Fig. 1b–d). Furthermore, these Klrg1+ iNKT cells from T-bet KO mice immunized with DC/Gal did not express Zeb2 (Fig. 1bright), indicating that T-bet is essential for Zeb2 induction in activated iNKT cells. To investigate the potential role of Zeb2 in iNKT-cell activation, we generated CD4-Cre Zeb2f/f (Zeb2-cKO) mice that lack Zeb2 activity specifically in T cells. The frequency of conventional CD4+ and CD8+ T cells in the spleen of Zeb2-cKO mice was normal (Supplementary Fig. 2a). The frequency of iNKT cells and the expression intensity of invariant TCR expression in iNKT cells in spleen were similar among wild-type mice, CD4-Cre negative Zeb2f/f littermate mice, and Zeb2-cKO mice (Fig. 1c). In addition, we analyzed whether steady-state iNKT cells develop normally in the lungs of Zeb2-cKO mice. We failed to detect any significant differences in the number and cytokine expression of steady-state lung iNKT cells in both WT and Zeb2-cKO mice (Supplementary Fig. 2b, c). Next, we examined the generation of Klrg1+ iNKT cells. The frequency and cell number of Klrg1+ iNKT cells in the lung and spleen of WT mice was increased a week after administration of DC/Gal (Fig. 1d–f). In contrast, the frequency of Klrg1+ iNKT cells in Zeb2-cKO mice was significantly lower than in WT mice and in mice with a floxed Zeb2 allele lacking the Cre transgene (littermate controls) (Fig. 1d–f). We previously reported that in DC/Gal-immunized mice, the expression of Tbx21 in Klrg1+ iNKT cells is considerably higher than that in Klrg1− iNKT cells17. We subsequently compared the expression of Tbx21 in Klrg1+ or Klrg1− iNKT cells between DC/Gal-injected Zeb2-cKO and WT mice. (Fig. 1g). Our findings revealed that the expression of Tbx21 in the Klrg1+ iNKT cells of DC/Gal-injected WT mice was higher than that in DC/Gal-injected Zeb2-cKO mice. In contrast, the expression of Tbx21 in the Klrg1+ cells of DC/Gal-injected Zeb2-cKO mice was similar to that in the Klrg1− iNKT cells of DC/Gal -injected WT and Zeb2-cKO mice (Fig. 1g). In summary, the frequency of Klrg1+ iNKT cells was considerably lower in Zeb2-cKO mice after administration with DC/Gal (Fig. 1e). Additionally, the expression of T-bet in the Klrg1+ iNKT cells in DC/Gal-injected Zeb2-cKO mice was much lower than that of Klrg1+ iNKT cells in DC/Gal-injected WT mice (Fig. 1g).
Zeb2 is required for the differentiation of Klrg1+Cx3cr1+GzmA+ iNKT cells
Previously, we demonstrated that Klrg1+ iNKT cells express granzyme A (GzmA) exclusively16. Therefore, we initially confirmed high expression of Gzma transcripts in sorted Klrg1+ iNKT cells, but not Klrg1− iNKT cells using real-time PCR from the lungs after administration of DC/Gal (Fig. 2a). Murray et al. reported that Cx3cr1 is also a marker for the antigen-exposed effector, NKT1 type Klrg1+ iNKT cells13. Therefore, we assessed GzmA and Cx3cr1 expression levels in Klrg1+ iNKT cells by flow cytometry. Most Klrg1+ iNKT cells expressed GzmA and Cx3cr1 in the lung, but were also detected in the spleen at a lower frequency (Fig. 2b, c). While the frequency of Cx3cr1+ GzmA + in Klrg1+ iNKT cells in WT and littermate control mice on day7 after DC/Gal administration was detected at approximately 40–50%, these populations were mostly diminished in Zeb2-cKO mice (Fig. 2d).
In Zeb2-deficient conventional T cells, Klrg1loCD127hi memory precursor cells increased during viral infection20,21. However, expression pattern of Klrg1 and CD127 has not been analyzed in iNKT cells. Klrg1+ iNKT cells downregulated expression of CD127 in WT and Zeb2-cKO mice immunized with DC/Gal (Fig. 2e). We next examined whether Klrg1+ iNKT cells expressed markers of T-cell exhaustion. Klrg1+ iNKT cells did not exhibit higher expression of PD-1, CTLA4, TIM3, and Lag3 (Fig. 2f). Therefore, the data indicate that Klrg1+ iNKT cells are not likely to be exhausted cells.
It has been shown iNKTFH are generated after an administration with α-GalCer28,29. Consistent with previous reports, our experimental setting also generated PD-1+Cxcr5+ iNKTFH cells in WT as well as Zeb2-cKO mice (Fig. 2g). These results suggest Zeb2 plays a selective role in driving differentiation of Klrg1+ Cx3cr1+GzmA+ iNKT cells.
Normal development of iNKT-cell subsets in the thymus in Zeb2-deficient mice
As shown in Supplementary Fig. 2b, the absolute cell numbers of iNKT cells in the thymus of WT and Zeb2-cKO mice were comparable. Next, we carefully assessed whether Zeb2 plays a role in the development of iNKT cells in the thymus. Closer analysis of the thymus revealed that the frequency and absolute number of CD24+CD44−stage 0, CD24−CD44−NK1.1−stage 1, CD44+NK1.1−stage 2, and CD44+NK1.1+stage 3 were similar in WT mice, littermate control, and Zeb2-cKO mice (Fig. 3a, b). We analyzed the expression of transcription factors related to iNKT-cell differentiation, such as T-bet, Gata3, Rorγt, of NKT1, NKT2 and NKT17 subset respectively, and the master regulator (PLZF) of all iNKT cells. The development of iNKT-cell subsets was almost the same among WT, littermate control, and Zeb2-cKO mice (Fig. 3c–e). Therefore, these results suggest Zeb2 does not affect the development of iNKT-cell subsets in the thymus. In fact, when the expression of Zeb2 in each stage of iNKT cells in the thymus is examined, Tbx21 expression is upregulated as the stage progresses, whereas Zeb2 expression is barely observed (Fig. 3f).
Two recent papers separated NKT1 subset into three subsets of NKT1 in steady state of the thymus and peripheral tissues: C0 iNKT cell30 (iNKT1a31), C1 iNKT cell(2B4−)30 (iNKT1b31) and C2 iNKT cell(2B4+)30 (iNKT1c31). In the current study, we verified the NKT1 subsets in WT and Zeb2-cKO mice similarly (Supplementary Fig. 2d, e). Furthermore, having sorted C1 iNKT and C2 iNKT cells from the thymus of WT mice, each type was separately transferred to Rag−/− mice (Supplementary Fig. 2f), and on day 5 after the transfer, DC/Gal were administered. Subsequent analysis of Klrg1+ iNKT cells in the lungs 1 week later revealed the expression of Klrg1 in the C1 iNKT and C2 iNKT cells-transferred mice (Supplementary Fig. 2g), thereby indicating that both C1 and C2 iNKT cell subsets can undergo differentiation to Klrg1+ iNKT cells.
Zeb2 maintains long-lived Klrg1+ iNKT effector cells
Because Klrg1+ iNKT cells in the lung were maintained for a long period as effector memory several months after activation16, we next examined the role of Zeb2 in the longevity of effector iNKT cells 4 weeks after DC/Gal administration. The frequency of iNKT cells in the lungs in Zeb2-cKO mice were almost similar to those of WT mice (Supplementary Fig. 3a). Klrg1+ iNKT cells in the lungs of DC/Gal-immunized WT mice and littermate controls were maintained with relatively high frequency compared to the spleens of DC/Gal-immunized WT mice and littermate controls (Fig. 4a, b). One month after immunization the Klrg1+ iNKT cells exhibited a CD27loCD43hiCD62L−CD69−CD122+NK1.1+NKG2DhiLy6Chi CD49d+ phenotype, which are different from iNKT cells in the steady state (Supplementary Fig. 3b). A lower frequency of Klrg1+ iNKT cells was detected in Zeb2-cKO mice (Fig. 4a, b), and then Klrg1+ Cx3cr1+GzmA+ iNKT cells were greatly diminished (Fig. 4c, d). These findings thus indicate that Zeb2 plays a role in the differentiation of NKT1 cells from activation to the maintenance of long-term effector Klrg1+ Cx3cr1+ GzmA + iNKT cells in peripheral tissues.
The generation of Klrg1+ long-lived iNKT effector cells was independent of the thymus
We suspected whether newly recruited iNKT cells from the thymus were continuously activated or that iNKT effector cells were differentiated to a long-lived form in peripheral tissues independent of the thymus. Thymectomized mice or sham-treated mice were administered DC/Gal two weeks later. Four more weeks later, the frequency and number of iNKT cells were evaluated in the lungs and spleen (Fig. 5 and Supplementary Fig. 4). The frequency and cell number of total iNKT cells in the lung were decreased in DC/Gal-administered thymectomized mice compared with DC/Gal-injected sham mice (Fig. 5a and Supplementary Fig. 4a). However, the percentages and cell numbers of Klrg1+ iNKT cells, particularly Cx3cr1+ GzmA+ effector iNKT cells, were comparable. (Fig. 5b–e and Supplementary Fig. 4b). These findings suggest that Klrg1+ iNKT cells were not replenished from the thymus, but were maintained in the peripheral tissue.
Single-cell transcriptome analysis of Klrg1+ iNKT cells from WT and Zeb2-cKO mice
The question arose as to why Klrg1+ iNKT appeared transiently at a low frequency in Zeb2-cKO mice after activation, and disappeared later. In order to elucidate the heterogeneity and differentiation trajectory of lung Klrg1+ iNKT cell population, we sorted lung Klrg1+ iNKT cells one week after immunization with DC/Gal, and analyzed them using single-cell transcriptome analysis. After integrating iNKT cells from littermate control and Zeb2-cKO mice, the data were dimensionally reduced by UMAP with unbiased clustering (Supplementary Fig. 5). In fact, UMAP plot showed unbiased total of 12 clusters (Supplementary Fig. 5). To identify the cell state of each cluster, mean read counts and the ratio of mitochondrial genes were calculated. Αfter removing dead cells, doublets, or multiplets in addition to a non-NKT cluster, we found a total of eight clusters of iNKT cells (Fig. 6a). Next, the cluster frequency was analyzed (Fig. 6b). Clusters C1, 9, 10, and 11 were found only in littermate control, whereas C2 was found only in Zeb2-cKO; C3 and C4 had a higher ratio in KO than in littermate control; and C7 was comparable. This suggests that C2, 3, 4 and 7 were independent of Zeb2. We analyzed gene signatures in each cluster of Klrg1+ iNKT cells and represented themt as a heatmap (Fig. 6c). C4 is Gata3hi Tbx21lo IL17rbhi, typical of the NKT2 subset. C3 was unclassified because it expressed mixed gene signatures (Tbx21lo Lef1hi Plac8hi Gata3hi Fhl2hi CXCR6hi) of immature NKT1 and NKT2 subsets. We found that Klrg1+ iNKT cells on day 7 included some NKT2 (C4) and unclassified cells (C3) in a small population. Given that in both WT and Zeb2-cKO mice, NKT2 cells were clearly detected in C4, this indicates that the differentiation of NKT2 to Klrg1+ iNKT cells is not dependent upon Zeb2. Similarly, the unclassified Klrg1+ cells of C3 were identified as Zeb2-independent cells. By contrast, the main cluster C2 of Zeb2-cKO Klrg1+ iNKT expressed a low RNA content and low expression of mitochondrial-related genes, suggesting a dying population (Supplementary Fig. 5).
In contrast, C1, C7, C9, C10, and C11 were in the NKT1 lineage. We verified that many iNKT cells among NKT1 clusters expressed Zeb2 as well as Klrg1 (Supplementary Fig. 6). The trajectory analysis using psylingshot showed that the differentiation goes in the following order, i.e., C7 → C11 → C1 → C9 → C10 (Fig. 6d). Zeb2 expression was relatively low at C7 as the starting point, peaked at C9, and decreased at C10 (Fig. 6e). Patterns of differentially expressed genes between these clusters in NKT1 lineages supported this trajectory pattern. For instance, C7 exhibited CD5hi, which reflects TCR signal, and high expression of NF-κB pathway genes and C9 showed a high level of gene expression of cytotoxic molecules and chemokines like NK cells (Fig. 6f). In Klrg1+ iNKT cells from Zeb2-cKO mice, C11, C1, C9 and C10 were almost lost, suggesting the differentiation is inhibited from C7 to C11 in the absence of Zeb2. Instead, C7 skewed to C2 showing dying signatures. Long-lived Klrg1+ Cx3cr1+GzmA+ iNKT cells originated from C11, C1, and C9. Furthermore, besides GzmA, Cx3cr1, Ccl5, Klf2, Klf3, and GzmB also behaves similarly with Zeb2 (Fig. 6e). Therefore, we clarified the branch point of iNKT-cell differentiation where Zeb2 functions to drive differentiation from C7 to C11 and protect precursors from apoptotic pathway. Thus, we showed that whereas Zeb2 plays a role in the differentiation of NKT1 cell into the memory phase, it does not influence the differentiation of NKT2 subset cells.
Zeb2 deficient Klrg1+ NKT cells underwent apoptosis
We checked the protein level to determine whether Klrg1+ iNKT cells in DC/Gal-injected Zeb2-cKO mice induced apoptosis (Fig. 7). When the cell proliferation marker Ki-67 and apoptosis Annexin-V were examined in DC/Gal-injected WT and Zeb2-cKO mice 7 days after immunization, the expression of Ki-67 in the Klrg1+ iNKT cells was lower (Fig. 7a) and more Annexin-V-positive in the Klrg1+ iNKT cells were present in DC/Gal-injected Zeb2-cKO mice than those in the DC/Gal-immunized WT mice (Fig. 7b), indicating that Klrg1+ iNKT cells are apoptotic and cannot be maintained when Zeb2 is deficient. In contrast, however, we did not detect any significant differences in Ki-67+ T cells or Annexin-V-positive T cells in the TCRβ+tetramer− T cells between WT and Zeb2-cKO mice (Fig. 7a, b). Together, consistent with the single-cell transcriptome results, Klrg1+ iNKT cells were apoptotic and unmaintainable when Zeb2 was deficient (Fig. 7c).
Secondary boosting effect in Zeb2-cKO mice
Finally, we also sought to determine whether an antigen-specific secondary response could be maintained in DC/Gal-vaccinated Zeb2-cKO mice (Fig. 8). Mice were rechallenged with DC/Gal 7 months after the first vaccination and analyzed 1 week later. The total number of Klrg1+ iNKT cells in WT mice after boosting with DC/Gal was almost three times higher than in WT mice with DC/Gal immunization, consistent with a previous report16. The number of Klrg1+ iNKT cells in vaccinated Zeb2-cKO mice did not increase after the boost with DC/Gal, but was like the primary response. The recall response, which indicates the rapid response of pre-existing memory cells by re-exposure to the same antigen, was maintained in WT mice, but not in Zeb2-cKO mice. Therefore, Zeb2 may play an important role in the memory formation of Klrg1+ iNKT cells.
Discussion
In this study, we determined the role of Zeb2 in antigen-driven differentiation of iNKT cells in peripheral tissues. Zeb2 did not affect the differentiation of NKT1, NKT2, or NKT17 cells in the thymus, but significantly affected the generation of long-lived effector Klrg1+ iNKT cells from the NKT1 subset in the lungs after antigen stimulation. In our previous reports on mice immunized with DC/Gal, Klrg1+ iNKT cells were characterized by the expression of several markers, including the CD43hiCD49dhiNKG2Dhi GzmA + phenotype, Ccl3, Ccl4, IFN-γ, and Tbx2116. Using RNAseq and ATAC-seq, Murray et al. confirmed that iNKT effector cells showed Klrg1+ GzmA + Cx3cr1+IFN-γ+13. Both findings suggest that they are derived from the NKT1 subset. In this study, we also verified that Klrg1+ prolonged effector iNKT cells were obtained from the NKT1 subset in a Zeb2-dependent manner. Regarding the steady state of iNKT cells, two recent studies demonstrated that naïve NKT1 cells were separated into three subsets in the thymus and peripheral tissues: C0 (iNKT1a), C1 (iNKT1b), and C2 (iNKT1c)30,31. The C2 (iNKT1c) subset shows higher levels of cytotoxic molecules and IFN-γ expression. These three NKT1 subsets were maintained in the steady state in the presence of IL-15. In contrast to cytokine signaling, TCR stimulation with DC/Gal can differentiate naïve NKT1 cells to long-lived iNKT cells. We verified that both C1 iNKT1 and C2 iNKT1 subsets from thymus differentiated into Klrg1+ iNKT cells in the periphery, i.e., lung after administration with DC/Gal.
The persistent expression of Klrg1, which is enforced by T-bet, Zeb2, and Id2, has been associated with senescence and terminal differentiation20,21,32. During acute infection, effector CD8+ T cells are divided into two populations: the Klrg1−CD127hi (MPEC) population is supposed to form a long-lived memory pool, whereas the Klrg1+CD127low (SLEC) population comprises short-lived effector cells33,34. In contrast, some Klrg1+CD127low CD8+ T cell population persists in the memory phase and exhibits robust protective functions during rechallenge with bacteria or viruses35,36. These CD8+ T cells are termed long-lived effector cells (LLEC). LLEC, which is a circulating memory population, expresses Klrg1, GzmA and Cx3cr1; this profile resembles the Klrg1+ iNKT cells.
Although Zeb2 is involved in the regulation of NK cells and T cells, there are similarities and differences in their activation signals and the maintenance of memory function. First, Zeb2 is required for NK cell differentiation. Zeb2-deficient NK cells were found to have impaired differentiation into the CD27−CD11b+terminal maturation stage due to NK apoptosis22. By contrast, Zeb2 had no specific effect on T-cell differentiation in the steady state. However, in LCMV infection models, Zeb2-deficient CD8+ T cells show a marked decrease in antigen-specific CD8+ T cells in the effector phase20,21. The generation of Klrg1(hi) effector memory T-cell populations was significantly altered, and Zeb2-deficient memory CD8+ T cells were reduced in secondary reactions20. In the case of iNKT cells, like T cells, Zeb2-deficient iNKT cells can develop normally in the thymus, at a normal frequency. However, apoptosis of some of these cells was revealed during differentiation after activation, similar to that of NK cells.
Several studies suggest that T-bet, a transcription factor, may regulate Zeb2 expression in T cells and NK cells20,21,22; furthermore, Zeb2 expression is implied in the importance of c-Myb37. T-bet binds to the Zeb2 locus to induce Zeb2 expression and both transcription factors appear to be required for optimal binding to the T-bet-regulated cis-regulatory region. By contrast, c-Myb-mediated repression of Zeb2 may involve interaction with the coactivator p300 via the induction of repressive non-coding RNA37. Because we did not find an enhancement in Zeb2 expression in Klrg1+ iNKT in T-bet KO mice, Zeb2 expression must be regulated by T-bet in the NKT1 subset, as well as in T cells and NK cells. The antagonistic relationship between the actions of the transcription factors Zeb1 and Zeb2, and micro RNA (miR)-200 regulates and determines the memory capacity of effector T cells. In this circuit, Zeb1 with miR-200 family microRNAs repress Zeb2 expression38. Runx3 also suppresses the high expression of T-bet and Zeb2, which normally occurs in effector T cells39.
It is believed that iNKT cells express activation markers in the steady state and can immediately respond to foreign substances upon ligand stimulation. On the other hand, several recent reports have indicated that not all iNKT cells return to a steady state after activation13,16,17. However, the process has not been elucidated. In this study, we performed a single-cell transcriptome analysis of Klrg1+ cells from DC/Gal-immunized WT and Zeb2-cKO mice. We found that the NKT1 lineage in Klrg1+ iNKT cells comprised several clusters: C7, C11, C1, C9, and C10. The present results provide new evidence using a single-cell transcriptome analysis that Zeb2 is critical for the differentiation to C11 from C7 without undergoing apoptosis. In fact, when WT and Zeb2-deficient Klrg1+ iNKT cells were compared in terms of apoptosis (Annexin-V) and proliferation (Ki-67) at day 7, Zeb2-deficient Klrg1+ iNKT (i.e., Klrg1+ Cx3cr1−GzmA− iNKT) cells were more prone to apoptosis at the differentiation stage. Thus, Zeb2 is critical for the establishment of Klrg1+ long-lived effector iNKT cells. The role of Zeb2 in iNKT cells is similar in some respects to its role in CD8+ T cells and NK cells, and dissimilar in others. Furthermore, Klrg1+ iNKT cells were also negative for PD-1, CTLA4, and Lag3, and could be enhanced by secondary effects, indicating that Klrg1+ iNKT cells are not exhausted iNKT cells, but Zeb2-dependent long-lived effector iNKT cells. Among innate lymphocytes, an induction of long-term effects of effector memory like innate lymphocytes may lead to strengthened immune surveillance against tumor cells.
Methods
Study design
The main objective of this study was to elucidate the intrinsic mechanisms of long-lived effector iNKT cells, which express Klrg1 and have the capacity of memory response and anti-tumor effects, mediated via ligand-specific immunization. Our specific hypothesis in this regard was that these Klrg1+ iNKT cells can be memorized during the priming phase in peripheral organs via selective mechanisms associated with activation, which is regulated by a transcription factor.
On the basis of screening candidate transcription factors specifically expressed in Klrg1+ iNKT cells using RNA-seq, we identified Zeb2 as the putative transcription factor. To investigate the potential role of Zeb2 in iNKT cell development and activation, we generated CD4-Cre Zeb2f/f (Zeb2-cKO) mice that specifically lacked T cell Zeb2 activity. Using these Zeb2-cKO mice, following antigen priming, we performed multi-modal analysis of iNKT development in the thymus and the differentiation of NKT cells in the periphery, and compared the effects with those detected among littermates or WT mice. To assess the kinetics and memory function of long-lived NKT cells, we conducted thymectomy and single-cell RNAseq analysis. Thymectomy was performed to distinguish between iNKT cells newly recruited from the thymus and those differentiated in the periphery. To elucidate the heterogeneity and differentiation trajectory of the Klrg1+ iNKT cell population, we performed scRNAseq of Klrg1+ iNKT cells in the activated phase after immunization. The sample sizes in all experiments deemed sufficient for statistical analysis were determined based our previous studies and preliminary experiments.
Mice
Specific pathogen-free 6–8-week-old C57BL/6 female mice were purchased from Charles River, Japan. B6;129(Cg)-Zeb2 < tm1.1Yhi > (Zeb2 flox/flox) mice were purchased from RIKEN BRC with permission from Dr. Y. Higashi (Osaka University)40. CD4-Cre Tg mice were kindly provided by Dr. Nakayama (Chiba University, Japan). T-cell-specific Zeb2 KO mice were obtained by crossing Zeb2flox/flox mice with CD4-Cre Tg mice. Rag1 KO (B6.129S7-Rag1tm1Mom/J) mice were purchased from the Jackson Laboratory. All mouse strains were backcrossed with C57BL/6 mice for 12 generations. B6.129S6-Tbx21 <tm1Glm > /J (T-bet KO) mice were purchased from the Jackson Laboratory. Most of the mice used herein were 6–12 week-old female mice. All mice were maintained under specific pathogen-free conditions at the RIKEN animal facility, and all procedures were performed in compliance with protocols approved by the Institutional Animal Care Committee at RIKEN (AEY2022-020).
Thymectomy
Mice at 6–8 weeks of age were anesthetized and shaven around the throat. A small incision was made at the base of the manubrium for suction cannula insertion. Both thymic lobes were removed separately. For sham surgery, mice were operated upon to make a small incision, although the thymus was not removed After more than two weeks of post-operative recovery, the mice were immunized with DC/Gal.
Cell preparation
The thymus and spleen were homogenized through a 70 μm cell strainer, and the erythrocytes were lysed using ACK lysing buffer (GIBCO), followed by two washes in RPMI1640 (SIGMA). For the isolation of lung and liver mononuclear cells (MNCs), the minced tissues were digested with collagenase D (Roche) and then layered on Percoll gradients (40/70%) (Amersham Pharmacia Biotec), followed by centrifugation for 20 min at 1840 × g. Bone marrow-derived dendritic cells (BM-derived DCs) were generated from BM precursors in culture with GM-CSF and pulsed α-galactosylceramide (α-GalCer) (200 ng/mL) for 48 h from day 6 and matured by LPS as previously described3. One million α-GalCer-pulsed mature BM-DCs (DC/Gal) were intravenously injected into one mouse.
Flow cytometry
The following monoclonal antibodies (mAbs) were purchased from BD Biosciences, BioLegend, Thermo Fisher Scientific, abcam, and Santa Cruz Biotechnology: anti-mouse-CD4 (GK1.5), -CD8 (53.67), -CD11a (M17/4), -CD11b (M1/70), -CD16/32 (93), CD19 (6D5), -CD24 (M1/19), -CD27 (LG.3A10), -CD43 (S7), -CD44 (IM7), -CD45 (30-F11), -CD49d (R1-2), -CD62L (MEL-14), -CD69 (H1.2F3), -CD103 (2E7), -CD122 (TM-b1), -CD127 (A7R34), -CD244.2 (eBIO244F4) -CD279 (29F.1A12), -CCR6 (29-2L17), -CXCR5 (2G8), -CXCR6 (SA051D1), - Cx3cr1 (SA011F11), -NK1.1 (PK136), -NKG2D (CX5), -Ly-6C (AL-21), -Klrg1 (2F1), -TCRβ (H57-597), -Glut1 (EPR3915), -granzyme A (3G8.5), -T-bet (eBio4B10), -Gata3 (TWAJ), -RORγt (B2D), -PLZF (9E12), -Eomes (Dan11mag), -TCF-1 (S33-966), -Blimp-1 (5E), -Ki-67 (SolA15), and streptavidin-FITC, -BV605, -BV650, BUV395. PE- or APC-conjugated CD1d-tetramers were purchased from MBL. Biotin-conjugated Annexin-V was purchased from BioLegend. Fixable Aqua Dead Cell Stain Kit (Invitrogen) and Fixable Violet Dead Cell Stain Kit (Invitrogen) were used to eliminate dead cells. Intracellular staining for transcription factors was performed using a eBioscience Foxp3 Staining Buffer Kit. Intracellular staining for cytokines was performed using a BD Cytofix/Cytoperm Kit. The concentrations of the antibodies used are presented in Supplementary Table 1. The LSRFortessa X-20 instrument and FlowJo software were used for analysis.
Cell sorting
Lung iNKT cells (Klrg1+ or Klrg1− TCRβ+CD1d-tet+) from non-immunized or DC/Gal-immunized mice were sorted using a FACSAria sorter (BD Biosciences) and used for qPCR. C1 (2B4− CXCR6+ TCRβ+CD1d-tet+) and C2 (2B4+ CXCR6+ TCRβ+CD1d-tet+) iNKT cells from the thymus of naïve WT mice were sorted for transfer experiments. Lung Klrg1+ iNKT cells were sorted from DC/Gal-immunized CD4-Cre negative Zeb2f/f littermate mice or Zeb2-cKO mice on day7 for scRNA-seq.
Quantitative PCR assay
To evaluate gene expression by iNKT cells, we performed cDNA synthesis and pre-amplification using RNA extracted from FACS-sorted iNKT cells (Klrg1+ or Klrg1− iNKT cells) without purifying RNA, using a CellsDirect One-Step qRT-PCR Kit (Invitrogen) with a mixture of pooled gene-specific primers (0.2 μM each). After 18 cycles of pre-amplification (each cycle: 95 °C for 30 s, 60 °C for 4 min), an aliquot was a template for quantitative PCR using the FastStart Universal Probe Master (Roche), a gene-specific forward and reverse primer pair, and the corresponding FAM-labeled hydrolysis probe (Universal Probe Library Set, Roche). Primers as follows: Ifng forward: 5′-ATCTGGAGGAACTGGCAAAA-3′, Ifng reverse: 5′-TTCAAGACTTCAAAGAGTCTGAGGTA-3′, Zeb2 forward: 5′-CCAGAGGAAACAAGGATTTCAG-3′, Zeb2 reverse: 5′-AGGCCTGACATGTAGTCTTGTG-3′, Gzma forward: 5′-GGCCATCTCTTGCTACTCTCC-3′, Gzmareverse: 5′-CGTGTCTCCTCCAATGATTCT-3′, Klrg1 forward: 5′-GGCTTGAGGAACATTGATGG-3′, Klrg1 reverse: 5′-TCAAGCTGTTGGTAAGAATCCTC-3′, Hprt1forward: 5′-TCCTCCTCAGACCGCTTTT-3′, Hprt1 reverse: 5′-CCTGGTTCATCATCGCTAATC-3′; probes as follows: Ifng (Roche Diagnostics 04686942001), Zeb2 (Roche Diagnostics 04688015001), Gzma (Roche Diagnostics 04685105001), Klrg1 (Roche Diagnostics 04685091001), Hprt11 (Roche Diagnostics 04692128001). Quantitative PCR was performed using ABI PRISM 7000 (Applied Biosystems).
In some experiments, we conducted alternative qPCR analysis. Total RNA was extracted from sorted cells using Trizol (Invitrogen,). The 1st strand cDNA was synthesized with PrimeScript II 1st strand cDNA Synthesis Kit (Takara). RT-qPCR was performed using TB Green Premix Ex Taq II (Takara,) and following manufacturer’s standard protocols. Primers as follows: Ifng forward: 5′-ATCTGGAGGAACTGGCAAAA-3′, Ifng reverse: 5′-TTCAAGACTTCAAAGAGTCTGAGGTA-3′, Il4 forward: 5′-CACTTGAGAGAGATCATCGGCA-3′, Il4 reverse: 5′-CCGAAAGAGTCTCTGCAGCTC-3′, Il17a forward: 5′-CAGGGAGAGCTTCATCTGTGT-3′, Il17a reverse: 5′-GCTGAGCTTTGAGGGATGAT-3′, Tbx21 forward: 5′-GCCCACAAGCCATTACAGGA-3′, Tbx21 reverse: 5′-AAATGAAACTTCCTGGCGCATC-3′, Zeb2 forward: 5′-CCAGAGGAAACAAGGATTTCAG-3′, Zeb2 reverse: 5′-AGGCCTGACATGTAGTCTTGTG-3, Hprt1forward: 5′-CCTCCTCAGACCGCTTTTT-3′, Hprt1 reverse: 5′-AACCTGGTTCATCATCGCTAA-3′.
Gene expression was measured using the ΔΔCT method, in which HPRT1 expression was an internal control.
Next-generation sequencing (RNA-seq)
We have deposited the RNA-seq data of lung iNKT cells from naïve or DC/Gal immunized mice in the Gene Expression Omnibus (GEO) database with accession code GSE128069.
Following quality control and trimming using trimgalore software (https://github.com/FelixKrueger/TrimGalore, version 0.6.7), the sequence reads were mapped and counted using Kallisto (https://pachterlab.github.io/kallisto/, version 0.48.0) to mm10 cDNA library provided by NCBI RefSeq. Expression changes and statistically significance of the difference were calculated using DESeq2 algorithm (https://github.com/mikelove/DESeq2, version 1.38.2). Transcription factors were defined as having “DNA binding”, “nucleus” and “regulation of transcription” annotations provided by the Gene Ontology database.
10x genomics library preparation
The scRNA-seq was prepared using Chromium Next Gel Bead In-Emulsion (GEM) Single Cell 5′ Reagent Kits v2 (10x Genomics, San Francisco, CA, USA). Briefly, FACS-sorted cells were washed once with PBS containing 0.1% bovine serum albumin. Approximately 6000 cells per sample were loaded onto Next GEM chip K (10x Genomics) and partitioned into GEMs in a Chromium Controller (10x Genomics). Single-cell RNA libraries were prepared according to the Chromium Next GEM Single Cell 5′ Reagent Kits v2 User Guide.
Sequencing
scRNA libraries were sequenced using a HiSeq X sequencer (Illumina) at a minimum sequencing depth of 20,000 reads/cell using read lengths of 26 bp for read 1, 8 bp for the i7 index 8 bp for the i5 index, and 90 bp for read 2.
scRNA-seq data processing
Single cell transcriptome analysis was performed using the software 10 x Genomics cellranger (https://support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome, version 6.1.2) following a standard protocol. mRNA sequences were mapped to mm 10 reference genome and de-multiplexed read counts were normalized, decomposed, and clustered using the Seurat toolkit (https://satijalab.org/seurat/, version 4.0.3). mRNA classification (nuclear/mitochondrial), marker gene detection, and other feature extraction of clustered cells were done using our in-house programs (see Resources).
Trajectory analysis
After removing dead, duplet, and non-NKT1 cells defined by gene expression pattern, NKT1 cells were re-decomposed using UMAP implemented in Seurat. Then, trajectories among NKT1 clusters were analyzed using pyslingshot (version 0.0.2, https://github.com/mossjacob/pyslingshot), a Python implementation of Slingshot (version 2.7.0, https://github.com/kstreet13/slingshot).
Statistics and reproducibility
The data are shown as the means±SEM. The number used for each experiment is shown in figure legends. The raw data related to this study has been presented in Supplementary Data 1. Statistical analyses were performed using StatMate (Ver 5.01). Differences were analyzed using a two-tailed Student’s t test or ANOVA Tukey–Kramer test. A two-tailed value of P < 0.05 was considered statistically significant.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Data availability
RNA-seq data that support the findings of this study have been deposited in the Gene Expression Omnibus (GEO) database with accession codes GSE12806917. The scRNA-seq data of iNKT cells have been deposited with links to BioProject accession number PRJNA1021694 in the DDBJ BioProject database. All source data underlying the graphs presented in the main figures are available in Supplementary Data 1.
Code availability
The code for data cleaning and analysis is provided as part of the replication package. It is available at https://github.com/takaho/nktsc for review.
References
Bendelac, A., Savage, P. B. & Teyton, L. The Biology of NKT Cells. Annu. Rev. Immunol. 25, 297–336 (2007).
Godfrey, D. I., Stankovic, S. & Baxter, A. G. Raising the NKT cell family. Nat. Immunol. 11, 197–206 (2010).
Fujii, S., Shimizu, K., Kronenberg, M. & Steinman, R. M. Prolonged interferon-γ producing NKT response induced with α-galactosylceramide-loaded dendritic cells. Nat. Immunol. 3, 867–874 (2002).
Godfrey, D. I., Uldrich, A. P., McCluskey, J., Rossjohn, J. & Moody, D. B. The burgeoning family of unconventional T cells. Nat. Immunol. 16, 1114–1123 (2015).
Legoux, F., Salou, M. & Lantz, O. Unconventional or preset alphabeta T Cells: evolutionarily conserved tissue-resident T cells recognizing nonpeptidic ligands. Annu. Rev. Cell Dev. Biol. 33, 511–535 (2017).
Griewank, K. et al. Homotypic interactions mediated by Slamf1 and Slamf6 receptors control NKT cell lineage development. Immunity 27, 751–762 (2007).
Lee, Y. J., Holzapfel, K. L., Zhu, J., Jameson, S. C. & Hogquist, K. A. Steady-state production of IL-4 modulates immunity in mouse strains and is determined by lineage diversity of iNKT cells. Nat. Immunol. 14, 1146–1154 (2013).
Engel, I. et al. Innate-like functions of natural killer T cell subsets result from highly divergent gene programs. Nat. Immunol. 17, 728–739 (2016).
Baranek, T., de Amat Herbozo, C., Mallevaey, T. & Paget, C. Deconstructing iNKT cell development at single-cell resolution. Trends Immunol. 43, 503–512 (2022).
Lee, Y. J. et al. Tissue-specific distribution of iNKT cells impacts their cytokine response. Immunity 43, 566–578 (2015).
Georgiev, H., Ravens, I., Benarafa, C., Forster, R. & Bernhardt, G. Distinct gene expression patterns correlate with developmental and functional traits of iNKT subsets. Nat. Commun. 7, 13116 (2016).
Lee, Y. J. et al. Lineage-specific effector signatures of invariant NKT cells are shared amongst gammadelta T, innate Lymphoid, and Th Cells. J. Immunol. 197, 1460–1470 (2016).
Murray, M. P. et al. Transcriptome and chromatin landscape of iNKT cells are shaped by subset differentiation and antigen exposure. Nat. Commun. 12, 1446 (2021).
Sun, J. C., Beilke, J. N. & Lanier, L. L. Adaptive immune features of natural killer cells. Nature 457, 557–561 (2009).
Sheridan, B. S. et al. γδ T cells exhibit multifunctional and protective memory in intestinal tissues. Immunity 39, 184–195 (2013).
Shimizu, K. et al. KLRG+ invariant natural killer T cells are long-lived effectors. Proc. Natl. Acad. Sci. USA 111, 12474–12479 (2014).
Shimizu, K. et al. Eomes transcription factor is required for the development and differentiation of invariant NKT cells. Commun. Biol. 2, 150 (2019).
Prasit, K. K. et al. Intratumoural administration of an NKT cell agonist with CpG promotes NKT cell infiltration associated with an enhanced antitumour response and abscopal effect. Oncoimmunology 11, 2081009 (2022).
Scott, C. L. & Omilusik, K. D. ZEBs: novel players in immune cell development and function. Trends Immunol. 40, 431–446 (2019).
Omilusik, K. D. et al. Transcriptional repressor ZEB2 promotes terminal differentiation of CD8+ effector and memory T cell populations during infection. J. Exp. Med. 212, 2027–2039 (2015).
Dominguez, C. X. et al. The transcription factors ZEB2 and T-bet cooperate to program cytotoxic T cell terminal differentiation in response to LCMV viral infection. J. Exp. Med. 212, 2041–2056 (2015).
van Helden, M. J. et al. Terminal NK cell maturation is controlled by concerted actions of T-bet and Zeb2 and is essential for melanoma rejection. J. Exp. Med. 212, 2015–2025 (2015).
Schlitzer, A. et al. Identification of cDC1- and cDC2-committed DC progenitors reveals early lineage priming at the common DC progenitor stage in the bone marrow. Nat. Immunol. 16, 718–728 (2015).
Scott, C. L. et al. The transcription factor Zeb2 regulates development of conventional and plasmacytoid DCs by repressing Id2. J. Exp. Med. 213, 897–911 (2016).
Wu, X. et al. Transcription factor Zeb2 regulates commitment to plasmacytoid dendritic cell and monocyte fate. Proc. Natl. Acad. Sci. USA 113, 14775–14780 (2016).
Scott, C. L. et al. The transcription factor ZEB2 is required to maintain the tissue-specific identities of macrophages. Immunity 49, 312–325.e315 (2018).
Huang, X. et al. Differential usage of transcriptional repressor Zeb2 enhancers distinguishes adult and embryonic hematopoiesis. Immunity 54, 1417–1432.e1417 (2021).
Chang, P. P. et al. Identification of Bcl-6-dependent follicular helper NKT cells that provide cognate help for B cell responses. Nat. Immunol. 13, 35–43 (2011).
King, I. L. et al. Invariant natural killer T cells direct B cell responses to cognate lipid antigen in an IL-21-dependent manner. Nat. Immunol. 13, 44–50 (2012).
Cui, G. et al. A circulating subset of iNKT cells mediates antitumor and antiviral immunity. Sci. Immunol. 7, eabj8760 (2022).
Baranek, T. et al. High dimensional single-cell analysis reveals iNKT cell developmental trajectories and effector fate decision. Cell Rep. 32, 108116 (2020).
Kaech, S. M. & Cui, W. Transcriptional control of effector and memory CD8+ T cell differentiation. Nat. Rev. Immunol. 12, 749–761 (2012).
Joshi, N. S. et al. Inflammation directs memory precursor and short-lived effector CD8(+) T cell fates via the graded expression of T-bet transcription factor. Immunity 27, 281–295 (2007).
Sarkar, S. et al. Functional and genomic profiling of effector CD8 T cell subsets with distinct memory fates. J. Exp. Med. 205, 625–640 (2008).
Olson, J. A., McDonald-Hyman, C., Jameson, S. C. & Hamilton, S. E. Effector-like CD8(+) T cells in the memory population mediate potent protective immunity. Immunity 38, 1250–1260 (2013).
Renkema, K. R. et al. KLRG1(+) memory CD8 T cells combine properties of short-lived effectors and long-lived memory. J. Immunol. 205, 1059–1069 (2020).
Gautam, S. et al. The transcription factor c-Myb regulates CD8(+) T cell stemness and antitumor immunity. Nat. Immunol. 20, 337–349 (2019).
Guan, T. et al. ZEB1, ZEB2, and the miR-200 family form a counterregulatory network to regulate CD8(+) T cell fates. J. Exp. Med. 215, 1153–1168 (2018).
Wang, D. et al. The transcription factor Runx3 establishes chromatin accessibility of cis-regulatory landscapes that drive memory cytotoxic T lymphocyte formation. Immunity 48, 659–674.e656 (2018).
Higashi, Y. et al. Generation of the floxed allele of the SIP1 (Smad-interacting protein 1) gene for Cre-mediated conditional knockout in the mouse. Genesis 32, 82–84 (2002).
Acknowledgements
We thank M. Kronenberg (La Jolla Institute for Immunology) for the critical reading of the manuscript. We also thank Masami Kawamura, Yuko Kurokochi and Jun Shinga for technical assistance and support in this study. This study was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI grants 26460583 (S.F. and K.S.) and funding from the RIKEN (RIKEN President’s Discretionary Fund) (S.F.).
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Conceptualization: S.F. and K.S. Methodology: T.I., K.S., T.W., T.E. and S.F., Investigation: T.I., K.S., H.A., M.S. and H.N. Visualization: T.I., K.S., T.W., T.E. and S.Y., Funding acquisition: S.F. Project administration: S.F., Supervision: S.F., Writing original draft & editing: K.S. and S.F., Writing and review: I.T.
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Iyoda, T., Shimizu, K., Endo, T. et al. Zeb2 regulates differentiation of long-lived effector of invariant natural killer T cells. Commun Biol 6, 1070 (2023). https://doi.org/10.1038/s42003-023-05421-w
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DOI: https://doi.org/10.1038/s42003-023-05421-w
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