Antibody-based therapeutics (ABTs) are used to treat a range of diseases. Most ABTs are either full-length IgG1 antibodies or fusions between for instance antigen (Ag)-binding receptor domains and the IgG1 Fc fragment. Interestingly, their plasma half-life varies considerably, which may relate to how they engage the neonatal Fc receptor (FcRn). As such, there is a need for an in-depth understanding of how different features of ABTs affect FcRn-binding and transport behavior. Here, we report on how FcRn-engagement of the IgG1 Fc fragment compare to clinically relevant IgGs and receptor domain Fc fusions, binding to VEGF or TNF-α. The results reveal FcRn-dependent intracellular accumulation of the Fc, which is in line with shorter plasma half-life than that of full-length IgG1 in human FcRn-expressing mice. Receptor domain fusion to the Fc increases its half-life, but not to the extent of IgG1. This is mirrored by a reduced cellular recycling capacity of the Fc-fusions. In addition, binding of cognate Ag to ABTs show that complexes of similar size undergo cellular transport at different rates, which could be explained by the biophysical properties of each ABT. Thus, the study provides knowledge that should guide tailoring of ABTs regarding optimal cellular sorting and plasma half-life.
Most approved antibody-based therapeutics (ABTs) are of the immunoglobulin G (IgG) isotype, and in particular the IgG1 subclass1,2. In addition, the IgG1 fragment crystallizable (Fc) region is used as a fusion partner to prolong the circulatory half-life of therapeutic proteins3,4,5. Both these ABT modalities are used to treat a range of diseases, including ocular diseases, hemophilia, type 2 diabetes, autoimmunity and cancer. Fc-fusion partners include coagulation factors (eftrenonacog alfa, efmoroctocog alfa) and hormone analogs (romiplostim, dulaglutide) used for replacement therapy, as well as receptor domains (aflibercept, etanercept, abatacept, belatacept) used to block disease drivers, such as vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF)-α. Importantly, IgG1 and Fc-fusions may bind and neutralize the same soluble targets. Examples include the VEGF-binding aflibercept and bevacizumab6,7, and the TNF-α binding etanercept, infliximab and adalimumab8. Having multiple ABTs for a given indication is beneficial for life-long treatment, as the therapeutic effect of each may decrease over time due to immune responses raised against a specific ABT9,10. In such cases, efficacy may be restored by switching to an alternative ABT11,12,13,14.
Long plasma half-life prolongs dosing intervals, which increases treatment adherence15,16. However, while IgG1s have a half-life of 3 weeks on average, individual monoclonal IgG1s have strikingly different half-lives, ranging from 6 to 32 days2,17. In addition, most IgG1 Fc-fusions have considerably shorter half-lives than full-length IgG1s, exemplified by a mean half-life of below 5 days for etanercept18, eftrenonacog alfa19 and romiplostim20. While this may in part be explained by the Ag-sink effect, these large differences must be related to their biophysical properties, as the ABTs are administered in excess amounts.
In vivo plasma half-life of ABTs relates to engagement of the neonatal Fc receptor (FcRn), which acts as a homeostatic regulator21,22. FcRn is expressed throughout the body, where it rescues IgG and Fc-containing molecules from degradation in both hematopoietic and non-hematopoietic cells23,24,25,26,27. Specifically, the receptor predominantly resides in acidified endosomes, where it encounters IgG entering the cells by fluid-phase pinocytosis. FcRn binds IgG at pH 5.0–6.5, triggering recycling of the complex to the cell surface, where exposure to the neutral pH of the extracellular milieu results in ligand release28,29,30,31. As such, the FcRn-dependent rescue mechanism is pH-dependent. Strict pH-dependent binding also drives FcRn-mediated transcytosis across polarized epithelial cell layers32,33. In addition, FcRn may protect IgG-containing immune complexes34,35, or facilitate processing of immune complexes by various immune cells in concert with the classical Fcγ receptors36,37,38.
While the principle binding site for FcRn is located to the Fc region39,40,41, the Fab arms modulate binding, and potentially also interact directly with the receptor42,43,44,45,46. In addition, the biophysical properties of ABTs, such as isoelectric point (pI) and surface charge distribution, may modulate both target binding and plasma half-life45,47,48,49,50,51. This raises the need for thorough studies of how distinct biophysical properties of different ABT designs relate to their FcRn engagement, intracellular transport and plasma half-life. Hence, we here report on such a study, where we combine biochemical and cellular studies with structural analyses and in vivo studies in human FcRn (hFcRn)-expressing mice to gain insights into how different ABT designs with distinct biophysical properties engage FcRn, undergo intracellular transport and behave in vivo.
An IgG1-derived Fc fragment exhibits short plasma half-life
We first used surface plasmon resonance (SPR) to compare FcRn-binding kinetics of a recombinantly produced IgG1-derived Fc fragment (50 kDa) and an IgG1 molecule (150 kDa), consisting of a human heavy chain paired with a mouse lambda light chain, with specificity for the hapten 4-hydroxy-3-iodo-5-nitrophenylacetic acid (NIP)52,53. IgG1 and Fc proteins were immobilized (≈400 RU), followed by injection of titrated amounts of monomeric receptor at pH 5.5. The obtained sensorgrams (Fig. 1a, b) were fitted to a 1:1 Langmuir binding model and yielded similar dissociation constants (KD) of 330 and 230 nM for IgG1 and the Fc fragment, respectively (Fig. 1d). Next, the proteins were injected on an FcRn-coupled affinity chromatography column at pH 5.5 (Fig. 1c), followed by elution with a gradual increase of pH to 8.8 to trigger receptor release54. This was done to mimic the endosomal pH-gradient, where FcRn engages IgG1 at acidic pH and releases it as the pH approaches neutral. Overlapping elution profiles were observed, with peaks at pH 7.11 and 7.13 for IgG1 and the Fc fragment, respectively.
To address how presence of the Fab arms affects plasma half-life, equimolar amounts of the proteins were intravenously injected into two distinct transgenic mouse strains expressing hFcRn, but at different levels, while lacking the mouse counterpart, namely homozygous and hemizygous Tg32 mice (Fig. 1e, f, h). Blood samples were collected for up to 30 days and plasma levels quantified by a two-way Fc-based ELISA. This revealed a 6.3-fold difference in half-life in Tg32 homozygous mice, corresponding to 12.7 days for IgG1 and 2.0 days for the Fc fragment. As expected, the half-lives were shorter in Tg32 hemizygous mice (8.5 vs. 1.8 days), but still, a 4.6-fold difference was measured. To assess the role of FcRn, the experiment was repeated in mice lacking FcRn (FcRn KO). Here, the half-life was only 39.1 h for IgG1, 7.8-fold shorter than that in Tg32 homozygous mice. However, the half-life was still 1.3-fold longer for full-length IgG1 than for the Fc fragment (Fig. 1g). Comparison of plasma concentrations 1 day after injection revealed that the elimination rate of the Fc fragment was higher than that of the IgG1 (Fig. 1h).
For further pharmacokinetic assessment, the serum concentrations of Fc and IgG1 were fitted to a non-compartmental pharmacokinetic model in MatLab55. Resulting values are listed in Supplementary Table 1. Fc had a higher clearance rate and volume of distribution than IgG1 in all three mouse models, likely due to its lower molecular weight. In Tg32 homozygous mice, the clearance of Fc was ~15-fold higher than that of IgG1, while in FcRn KO mice, this difference was reduced to 3.6-fold. Importantly, the Fc clearance in FcRn KO mice was 2.2-fold higher than in Tg32 homozygous mice, demonstrating that the Fc is also protected by FcRn-dependent mechanisms. Of note, the half-lives calculated by this method indicate a larger difference between the two molecules than what we observed experimentally.
Thus, despite the fact that FcRn binding kinetics, as assessed by SPR, and pH-dependent dissociation from FcRn, as assessed by chromatography, is similar for the two molecules, their serum half-lives were very different. The difference is partly FcRn dependent and partly determined by characteristics such as size and volume of distribution.
IgG1 and the Fc fragment are handled differently by cells
To compare the two molecules in a cellular context, we used a human endothelial cell-based recycling assay (HERA)56 (Fig. 1i), which takes advantage of an adherent human endothelial cell line stably overexpressing hFcRn57. Equimolar amounts of IgG1 and the Fc fragment were added to the cells followed by incubation for 3 h to allow for uptake. Next, the cells were washed and either lysed or placed in IgG-depleted growth medium, to allow for detection of cellular uptake, recycling or retention after additional 3 h, when the medium was collected and the cells washed and lysed. The two-way anti-Fc ELISA used for quantification demonstrated that 3.4-fold more of the Fc fragment than the IgG1 entered the cells during the uptake step (Fig. 1j), 5.3-fold more of the Fc was detected in the recycling medium (Fig. 1k), and 5.4-fold more had also accumulated inside cells at the termination of the assay (Fig. 1l).
To gain further insight into the uptake mechanism, we compared the amounts taken up after incubation for 30 min and 2 h, which revealed that 2.2- and 7.1-fold more Fc was taken up relative to IgG1 after the respective incubation times (Fig. 1m, n). Next, we addressed the FcRn contribution by treating the cells with siRNA targeting the FcRn heavy chain, using an established protocol56. Almost equal intracellular amounts of the two were measured after 30 min, and after 2 h, only 2.4-fold more of the Fc was detected. Thus, while the cellular uptake of the Fc fragment was largely dependent on FcRn (Fig. 1n), the uptake of the IgG1 was less so (Fig. 1m). To further study FcRn-dependent uptake of IgG1, we included an anti-NIP IgG1 Fc-engineered variant with five amino acid substitutions (M252Y/S254T/T256E/H433K/N434F; MST/HN) that improve FcRn-binding at both acidic and neutral pH, thereby enabling FcRn engagement at the cell surface53,58. As expected, this variant displayed an increased uptake relative to non-engineered IgG1, at a magnitude of 15-fold after 2 h incubation, and siRNA-treatment reduced its uptake by 4.7-fold (Supplementary Fig. 1a).
As FcRn also mediates transcytosis, we compared transcellular transport of the two molecules using a transwell system seeded with a Madin-Darby Canine Kidney (MDCK) cell line stably transfected with hFcRn (Fig. 1o). The proteins were added to the apical side and samples collected at the basolateral side 4 h later. Quantification by the two-way ELISA revealed 5.5-fold more of the Fc than IgG1 in the basolateral chamber (Fig. 1p). An anti-NIP IgG1 harboring three Fc amino acid substitutions that abolish receptor binding (I253A/H310A/H435A; IHH30,34) hardly reached the basolateral side (Fig. 1p).
In summary, the Fc fragment is internalized, recycled and transcytosed efficiently by an FcRn-dependent mechanism. In comparison, these mechanisms are less efficient for IgG1. Importantly, despite the high uptake and recycling of the Fc fragment, there is still a substantial residual intracellular fraction, resulting in degradation that likely contributes to its shorter half-life.
The nature of the Fc fusion partner affects pharmacokinetics
To investigate the effect of adding fusion partners other than anti-NIP Fab to the Fc region, we studied two ABTs with Fc-fused Ag-binding receptor domains, namely etanercept and aflibercept. Specifically, etanercept (MW ≈ 103 kDa) consists of the extracellular domains of TNF receptor 2 (TNFR2) fused to the IgG1 Fc, while aflibercept (MW ≈ 97 kDa) consists of the extracellular domain 2 of VEGF receptor (VEGFR) 1 linked to domain 3 of VEGFR2, creating a joint receptor domain fused to the IgG1 Fc which can bind both VEGF and placental growth factor59. First, plasma half-life was determined in Tg32 homozygote mice (Fig. 2a), where etanercept (3.6 days) and aflibercept (4.3 days) showed 1.8- and 2.2-fold extended half-life, respectively, compared to the Fc fragment (2.0 days). Furthermore, comparing plasma concentrations after 1 day revealed 12-fold more of etanercept than the Fc, but only 1.7-fold more of aflibercept (Fig. 2b). Additional pharmacokinetic parameters are listed in Supplementary Table 2, and show a maximum serum concentration of etanercept 26.8-fold higher than that of the Fc (64.4 vs. 2.4 µg/ml, respectively). Notably, the approximate two-fold longer half-life of these Fc-fusions compared to the IgG1 Fc could be due to their higher MW.
To address whether size could explain the longer half-life, we performed a similar comparison of IgG1 Fc and an additional Fc-fusion ABT with MW in the same range as aflibercept and etanercept, namely eftrenonacog alfa (MW ≈ 98 kDa), which consists of human coagulation factor IX fused to the IgG1 Fc (Supplementary Fig. 2). HERA parameters (Supplementary Fig. 2a–c) revealed this Fc-fusion to have an uptake roughly 3-fold increased relative to the Fc (Supplementary Fig. 2a), but recycling in the same range as the anti-NIP IgG1 (Supplementary Fig. 2b). This was reflected by the Fc-fusion displaying residual amounts nearly 3-fold that of the IgG1 Fc (Supplementary Fig. 2c), suggesting that the fusion partner confer increased cellular retention. Conversely, eftrenonacog alfa displayed circulatory properties near identical to that of the IgG1 Fc in Tg32 homozygous mice (Supplementary Fig. 2d, e).
Next, we compared the Fc-fusions with three full-length IgG1 variants, namely the anti-TNF-α IgG1s infliximab and adalimumab, as well as the anti-VEGF IgG1 bevacizumab. The IgG1s have clinical half-lives distinct from that of the Fc-fusions (ranging from 2–4 days for etanercept18 to ~20 days for bevacizumab60; summarized in Table 1). A schematic overview of their structural elements is shown in Fig. 3a. Despite some minor variations in amino acid sequence, highlighted in Supplementary Fig. 3e, both the Fc-fusions and the IgG1s contain the same Fc. Thus, the main variable is their Ag-binding moieties (ABMs) and the way they bind their cognate Ags (visualized in Supplementary Fig. 3a). While TNF-α forms a trimer in solution, VEGF is a dimer61,62. Solved crystal structures of each ABM, defined as fragment variables (Fvs) for IgG1s and receptor domains for Fc-fusions, are available through the Protein Data Bank (PDB), and are shown in Supplementary Fig. 4a. Of note, as no X-ray crystallographic structure of the aflibercept ABM has been solved, we visualized it by retrieving the structures of both its receptor domains and modeling their likely conformation by the use of Rosetta.
First, SPR-derived FcRn-binding kinetics of the ABTs at pH 5.5 were measured, which revealed KD values for all five in the range of 310–434 nM (Fig. 3b–f and Table 1). All eluted from the FcRn affinity chromatography column with peaks ranging from pH 6.88 to 7.21 (Fig. 3g and Table 1). Specifically, the Fc-fusions eluted before the IgG1s, and etanercept (6.88) before aflibercept (7.01). The IgG1s eluted from pH 7.15 (infliximab) to 7.20–7.21 (adalimumab and bevacizumab). Thus, the large differences in plasma half-lives observed for the five ABTs could not be predicted by differences observed in FcRn binding detectable by SPR or release from the receptor-coupled column.
IgG1s and Fc-fusions have distinct charge characteristics
Differences in cellular handling may be due to charge characteristics. In general, net positive charge may cause unspecific interactions with negatively charged cell membranes and off-target binding, while positive surface charge in Ag-interacting residues (AIRs) may delay release from FcRn45. Importantly, such patches may be partially or completely masked upon Ag-binding, which may change the overall charge of the immune complex. Last, but not least, the net charge of ABMs and AIRs may change as a function of pH. Net charge characteristics of the five ABTs and their cognate Ags at pH 5.5 and 7.4 are summarized in Table 1, and surface charge distribution and net charge of their ABMs throughout the endosomal pH-gradient are visualized in Supplementary Fig. 4b–f. For IgG1s, we determined AIRs as complementarity determining regions (CDRs). For receptor domains, we determined AIRs as amino acid residues within 5 Å of bound Ag in solved receptor domain-Ag co-crystal structures. Net and surface charge of VEGF and TNF-α are shown in Supplementary Fig. 3b–d.
These analyses show that the ABMs of both anti-TNF-α IgG1s display a positive charge at pH 5.5, as does that of the TNF-α receptor domain-fusion. In the AIRs, this positive charge is somewhat pH-dependent, but only for the IgG1s. TNF-α in itself is negatively charged at both pH 5.5 (−6.1) and 7.4 (−15.4), at a magnitude that ensures large net negative charge of all three anti-TNF-α ABMs after Ag-binding, regardless of pH.
The ABMs of the anti-VEGF IgG1 and VEGF receptor domain-fusion are also positively charged throughout the pH-gradient, and the VEGF receptor domain is more so than the other ABMs. In the anti-VEGF IgG1, AIRs are negatively charged at both pH 5.5 (−0.3) and 7.4 (−2.7), while the VEGF receptor domain of aflibercept has positively charged AIRs at both pH 5.5 (5.9) and 7.4 (4.2). As the net charge of VEGF at pH 5.5 is −2.1, The VEGF receptor domain will be net positively charged also after Ag-binding, while the corresponding anti-VEGF IgG1 immune complex will have negative charge. At pH 7.4, the net VEGF charge is −10.0, and both anti-VEGF IgG1 and receptor domain-fusion immune complexes will have net negative charge.
Fusion partner characteristics and pH affect Ag-binding and complex formation
In a clinical setting, the ABTs will bind and neutralize the activity of soluble Ags. Due to differences in the pH-dependent charge in their AIRs, we suspected that pH could affect their Ag binding differently. To address this, all five ABTs were injected over immobilized TNF-α or VEGF in SPR at pH 5.5 and pH 7.4. Acidic pH increased the on-rate of both the anti-TNF-α IgG1s relative to that observed at pH 7.4 (Fig. 4a, b), but not of the anti-TNF-α receptor domain-fusion (Fig. 4c). For VEGF, acidic pH enhanced the on-rate more than 5-fold of both the anti-VEGF IgG1 and anti-VEGF receptor domain-fusion (Fig. 4d, e). Thus, all ABTs with pH-dependent reduction of positive charge in the AIRs (Table 1) exhibited increased Ag-binding at acidic pH.
The size of the immune complexes resulting from ABTs binding to their cognate Ags is determined by the monomeric or multimeric state for the Ag, the availability of Ag-binding sites, and the properties of the ABT. We next addressed how the five ABTs formed complexes with their cognate Ags in solution. All were incubated with increasing concentration of the Ags at pH 5.5 and at pH 7.4, prior to injection on a high-resolution analytical size exclusion chromatography (SEC) column. We first observed the ABTs eluting as monomeric peaks with slightly different retention times in the absence of Ag. Then, in the presence of Ags, the three IgG1s formed large immune complexess that eluted earlier than the monomers at both pH conditions (Fig. 4f, g, j–l, o). At a 4-fold molar excess of Ag, multimerization was observed for all IgG1s.
In stark contrast, the two Fc-fusions exhibited only minor elution shifts in the presence of cognate Ag (Fig. 4h, i, m, n). For the anti-TNF-α receptor domain-fusion, heterogenous complex formation was observed at four-fold excess of Ag at pH 7.4, suggesting a mix of 1:1 and 1:2 complexes (Fig. 4m). For the anti-VEGF receptor domain-fusion, two distinct peaks were observed at pH 5.5 (Fig. 4i), but not at pH 7.4 (Fig. 4n). Thus, the IgG1s and Fc-fusions engaged their cognate Ags very differently, in that only the IgG1s formed large, multimeric immune complexes.
Ag binding alters binding to FcRn
To address FcRn-binding of Ag-bound ABTs, ABTs were preincubated with increasing concentrations of TNF-α or VEGF and injected over immobilized FcRn at pH 5.5 in SPR (Fig. 5a, c, e, g, i). The results in all cases showed FcRn binding in the presence of Ag, but with reduced peak responses and slower dissociation rates than for naked ABTs. This was more pronounced for the large IgG1 immune complexes (Fig. 5a, c, i) than the smaller Fc-fusion equivalents (Fig. 5e, g).
When Ag-bound ABTs were analyzed by analytical FcRn affinity chromatography, all three TNF-α-bound immune complexes again showed prolonged retention times (Fig. 5b, d, f). In comparison, a similar shift could not be detected upon VEGF binding (Fig. 5h, j). Thus, whether or not Ag-binding affects the pH-dependent dissociation from FcRn, does not depend on the size of the immune complexes formed, but rather their content.
Distinct differences in FcRn-mediated recycling of ABTs and immune complexes
To assess cellular handling of the ABTs and their immune complexes, we performed HERA, followed by detection in the two-way Fc-based ELISA. We found the two anti-TNF-α IgG1s and the anti-TNF-α receptor domain-fusion to have similar uptake efficiency in the absence of Ag (Fig. 6a). However, recycling of the IgG1s was 4-5-fold more efficient than that of the receptor domain-fusion (Fig. 6b). Comparing the anti-VEGF IgG1 and receptor domain-fusion revealed a two-fold higher uptake and a 1.3-fold higher recycling of the receptor domain-fusion (Fig. 6a, b). However, the residual amount of the anti-VEGF receptor domain-fusion was also high and its subsequent intracellular degradation will likely contribute to the short plasma half-life (Fig. 6c). Very little residual bevacizumab was observed. Thus, neither of the two receptor domain-fusions were as efficiently recycled as the IgG1s with corresponding specificities.
Next, prior to studying transport of Ag-bound ABTs, we addressed if adding TNF-α and VEGF to HERA affected cellular uptake and recycling. We mixed the anti-VEGF IgG1 with TNF-α at a 2-fold molar excess, and also one of the anti-TNF-α IgG1s together with VEGF (Supplementary Fig. 1c, d). As the results revealed no major impact of free Ag, the effect of immune complex formation was studied, by adding each of the five ABTs after preincubation with a two-fold molar excess of specific Ag. A summary of the resulting findings is shown in Table 2.
For the anti-TNF-α IgG1s, Ag-binding increased uptake by five-fold (Fig. 6a) and recycling more than seven-fold (Fig. 6b). In contrast, Ag-binding increased neither uptake nor recycling of the anti-TNF-α receptor domain-fusion (Fig. 6a). Thus, binding to TNF-α enhanced uptake, and subsequent recycling, of ABTs displaying (1) large immune complex formation and (2) a pH-dependent charge in AIRs. For the anti-VEGF IgG1 bevacizumab, Ag-binding resulted in a 14-fold increase in uptake (Fig. 6a), and a 9-fold increase in recycling (Fig. 6b). In comparison, Ag-binding yielded 7-fold increase in uptake and a 4-fold increase in recycling for the anti-VEGF receptor domain-fusion. Importantly, at termination of the assay, 2.3-fold more of VEGF-bound receptor domain-fusion than VEGF-bound IgG1 had accumulated intracellularly (Fig. 6c).
To study how the biophysical properties of ABTs and Ags affect cellular uptake independently of FcRn, we measured uptake after 2 h incubation in cells treated with FcRn-targeting siRNA in HERA. We added ABTs to cells in the absence (Fig. 6d) and presence (Fig. 6e) of Ag. We found the effect of FcRn-knockdown to vary somewhat between the ABTs, but the intracellular levels of all ABTs tended to decrease in the absence of Ag (Fig. 6d). Then, in the presence of Ag, uptake of only one of three IgG1s significantly dropped after siRNA treatment (adalimumab; Fig. 6e). Thus, immune complex size was not the determining factor. For the receptor domain-fusions, the effect of FcRn-knockdown differed, as the intracellular level of Ag-bound anti-TNF-α receptor domain-fusion decreased, while that of the anti-VEGF receptor domain-fusion was unaffected (Fig. 6e).
Fusion partners affect FcRn-mediated transcytosis
We next studied the ABTs in the MDCK-FcRn transwell system, and found both anti-TNF-α IgG1s to be transcytosed at rates higher than that of the anti-TNF-α Fc-fusion (Fig. 6f). Notably, the anti-TNF-α IgG1s differed, as the amounts of transcytosed adalimumab were 3.5-fold higher than infliximab. The anti-VEGF IgG1 and the corresponding Fc-fusion were transcytosed well and at similar levels.
Next, as VEGF and TNF-α are potent permeability factors63,64, we confirmed that neither transport of IgG1 nor transepithelial electrical resistance were drastically affected by adding the Ags to the medium (Supplementary Fig. 1h, i). This allowed studies of how immune complex formation affected transcellular transport in the MDCK-FcRn transwell system, and we found 2- to 4-fold increased transcytosis for all ABTs in the presence of their cognate Ags. In stark contrast to HERA, the hierarchy between the ABTs was largely unaffected by Ag-binding. Thus, the effect of Ag-binding on transcytosis and cellular recycling were distinct.
Varying pH and competition for FcRn-binding reveal distinct recycling capacities of Fc-fusions
Injected ABTs will compete with endogenous IgG for binding to FcRn. However, due to the absence of immunogenic stimuli in pathogen-free housing and the poor interaction between mouse Fc domains and hFcRn, hFcRn transgenic mice have low IgG levels65,66,67,68. To study the effect of competing endogenous IgG on cellular recycling, we modified the HERA protocol such that the assay could be carried out in the presence of so-called intravenous Igs (IVIg) pooled from human donors (illustrated in Fig. 7a). The protocol is described in the Supplementary Note 1, and in brief includes preloading cells with IVIg for 1 h at pH 7.4 prior to conducting HERA. As an additional modification, we performed the uptake step at pH 6.0, which enhances intracellular accumulation by allowing FcRn engagement at the cell surface and preventing receptor release during uptake56. Acidic pH also increases protein charge, which enhances uptake via electrostatic interaction with the negatively charged cell membrane. ABT amounts were quantified by Ag-specific ELISA, to distinguish the ABTs from the added IVIg.
We first addressed how changing the pH affected uptake and recycling. Briefly, comparing IgG1s to receptor domain-fusions demonstrated that acidic pH significantly increased the uptake of IgG1s, but not the receptor domain-fusions (Fig. 7b, c), at a magnitude of 2–3-fold. This was the case both in the absence (Fig. 7b) and presence of competition (Fig. 7c), and was mirrored by a 3–5-fold increase in recycled amounts of specifically the IgG1s (Fig. 7d, e). Thus, despite the fact that all ABMs display a pH-dependent charge, the pH modulated the cellular transport of ABTs with Fabs only. Interestingly, introducing competition did not drastically alter the hierarchy between the different ABTs in HERA (Fig. 7a–d).
Even so, the cumulative data suggested an impaired cellular recycling of the receptor domain-fusions. For the anti-TNF-α receptor domain-fusion, this was evident by its overall low amounts (Fig. 7a–d). For the anti-VEGF receptor domain-fusion, this was clear by its high relative uptake compared to the anti-VEGF IgG1 (Fig. 7a, b) not being mirrored by increased recycled amounts (Fig. 7c, d), as this signifies an increased amount of anti-VEGF receptor domain-fusion being degraded rather than recycled.
Therefore, we speculated that the receptor domain-fusions could be less potent competitors for cellular recycling than the IgG1s. To study the competitive ability of each ABT, we measured their capacity to reduce recycling of the anti-NIP IgG1. We did so by adding a constant amount of the anti-NIP IgG1 in the presence of titrated amounts of the ABTs at pH 6.0 to preloaded cells. After uptake, we added medium with pH 7.4 and incubated for 3 h to allow for cellular recycling. The amounts of anti-NIP IgG1 present in the collected medium were quantified by Ag-specific ELISA, which revealed that higher amounts of the receptor domain-fusions than IgG1s were required to reduce recycling of anti-NIP IgG1. Specifically, the IC50 value of both receptor domain-fusions were at least 2-fold that of the IgG1s with corresponding specificities (Fig. 7f, g), demonstrating a reduced capacity of receptor domain-fusions to undergo cellular recycling.
Strikingly, the Fc fragment demonstrated an IC50 of 32.9 nM, and thus reduced rescue of anti-NIP IgG1 more potently than the ABTs. Thus, the cumulative data demonstrates that the absence of Fc-fused moieties enhances not only intracellular accumulation and recycling, but also ability to compete for recycling.
FcRn is a key regulator of the circulatory half-life of IgG and all Fc-containing ABTs, due to its ability to bind to the Fc fragment in a pH-dependent fashion2,21,22. Even though the Fc harbors the principal binding site for FcRn, ABTs with identical Fc sequences, but distinct biophysical differences in their Fab arms or Fc-fusion partners, exhibit different plasma half-lives. This is a result of both FcRn-dependent and -independent factors2,4,45,69,70. While Fc-fusions in general have shorter plasma half-life than full-length IgG1s, little is known about their ability to engage FcRn and their cellular transport properties. Therefore, we conducted a comprehensive study of FcRn engagement and cellular transport of IgG1 Fc-based molecules.
When comparing plasma half-life of an IgG1 and its Fc fragment in hFcRn transgenic mice, we found it to be considerably shorter for Fc than for IgG1. Importantly, this does not stem from different in vitro binding kinetics to the receptor, as they bound equally well in SPR. Rather, the presence of the Fabs extends plasma half-life by a mechanism that involves FcRn expression on cells. Then, by the use of mouse strains with different hFcRn expression levels, we showed that the amount of FcRn present affects the extent of Fab-mediated half-life extension. We chose a hapten-specific chimeric mouse-human anti-NIP IgG1 as a model Ab, which had an 8.5-day plasma half-life in hemizygote Tg32 mice, in line with a previous report56. While the half-life of distinct IgGs varies in this model, most exhibit plasma half-lives in the range of 4–8 days71, which is at least 2-fold longer than the 1.8 days we measured for the Fc fragment. Interestingly, in a recent study of a human IgG4 Fc fragments engineered to lack FcRn-binding, a 5.6-fold reduction in plasma half-life in non-human primates was seen72. This study supports our finding that the Fc fragment (50 kDa), regardless of some renal elimination, is indeed rescued from degradation in an FcRn-dependent manner in vivo.
We found that intracellular uptake of the Fc fragment is more efficient than that of IgG1, and that the uptake is FcRn dependent. Recycling is also enhanced. However, as uptake increased more than recycling, we found the Fc fragment to accumulate within cells, and more so than IgG1. Intracellular degradation of this fraction would partly explain the short serum half-life of the Fc fragment.
Furthermore, as the Fc fragment is taken up more efficiently than IgG1, it potently outcompeted the IgG1 for cellular recycling. Last, but not least, the Fc fragment was transcytosed at an efficiency beyond that of IgG1 in polarized kidney cells. We speculate that this may be a direct effect of greater uptake of Fc on the apical side.
Recent reports highlight that pI and surface charge distribution of Abs may influence both FcRn-dependent and -independent parameters, and that high positive charge in particular may delay release from FcRn45,48,49,50. However, as we found our model IgG1 to have a more positive net charge than the Fc throughout the pH-gradient, positive charge cannot explain the increased uptake and subsequent transport of the Fc fragment. We postulate that it could rather be associated with sterical hindrance caused by Fc-fused structures upon binding to FcRn in the curved membrane of the endosomes. The Fc binds FcRn in a 1:2 stoichiometry in an orientation that brings the Fabs, and conversely, Fc-fused structures in general, into close proximity with the membrane39,73. Accounting for this, a two-pronged binding mechanism has been proposed, where the Fabs bend away from the membrane to interact with FcRn51. The ease at which Fc-fused structures can undergo such a reorientation upon binding to FcRn may affect the spatial orientation and stability of the Fc:FcRn complex in the endosome. As such, unfavorable biophysical characteristics of Fc-fused structures may impose on FcRn engagement. A sole Fc will have no such restrictions, which could explain its specific FcRn-related properties observed in our findings.
Consequently, we went on to compare the naked Fc fragment with Fc-fusions containing Ag-binding receptor domains. This revealed that neither receptor domain-fusion impair in vitro binding to FcRn, and that their presence rather increases plasma half-life 2-fold relative to that of the Fc. In comparison, in the same mouse strain, the model IgG1 had a half-life more than 6-fold longer than the Fc. This demonstrates that the presence of the Fab arms contributes to FcRn-dependent pharmacokinetics, in a manner distinct from that of Fc-fused receptor domain domains.
Notably, the anti-TNF-α receptor domain Fc-fusion etanercept displayed a near 12-fold higher concentration in plasma than both the Fc fragment and the anti-VEGF receptor domain Fc-fusion aflibercept 24 h after injection. This could be explained by high intracellular accumulation of the Fc fragment and aflibercept relative to etanercept. Both aflibercept and the Fc-fragment were internalized in an FcRn dependent manner, while etanercept chiefly stayed in solution. Interestingly, the high plasma levels of etanercept did not translate into a longer plasma half-life, as this was approximately the same as for aflibercept. Thus, distribution to predominantly plasma, rather than cells and tissues, does not necessarily prolong plasma half-life.
To learn more about how differences in the Fc-fused moiety affect FcRn-mediated processes, we included both the two receptor domain-fusions and three IgG1s targeting either VEGF or TNF-α in further studies. All had similar FcRn-binding properties at acidic pH in SPR and affinity chromatography, that is, in the absence of a membrane. Moving on to cellular studies, however, where the receptor is embedded in the membrane, we observed that while the anti-VEGF receptor domain Fc-fusion aflibercept, similar to the Fc fragment, was taken in and recycled quite efficiently, it also accumulated intracellularly. This allows for subsequent degradation. All three IgG1s were internalized and recycled efficiently. However, much less anti-VEGF IgG1 bevacizumab accumulated intracellularly. It was thus rescued efficiently, in line with it having a particularly long plasma half-life, twice that of the two other IgG1s.
Upon injection into patients, IgG1 Fc-based ABTs will compete for binding to FcRn in the presence of endogenous IgG, which has an average concentration in blood of 12 mg/ml. The fact that the half-life of IgG molecules is controlled by a concentration-catabolism relationship74, became evident by studies of IgG in myeloma patients and mice preloaded with IgG75,76. Lack of such competition prolongs half-life of IgG67,77, and while recent progress has been made to account for endogenous competition in transgenic mouse models76,78, in vivo studies of the impact of competition on different ABT designs are limited by ethical challenges, high cost and animal availability. Therefore, we established a HERA protocol where endogenous competition for the IgG-binding site of the receptor is included, which confirmed that receptor domain-fusions are less efficient than IgG1s in cellular recycling.
Notably, efficient cellular uptake of Abs correlates with high net positive charge, facilitating interaction with the negatively charged cell membrane. This is exemplified by the anti-p40 IgG1 briakinumab, which has large positive charge patches in the variable domains of its Fab arms that delay its release from FcRn at neutral pH45 and enhances its unspecific cellular uptake in endothelial cells79. This gives briakinumab a plasma half-life of only 8–9 days in humans80,81. In line with this, briakinumab displayed a high uptake in HERA, which was not significantly reduced upon siRNA treatment (Supplementary Fig. 1b). For our chosen ABTs, charge differences largely correlated with their cellular uptake. For example, the ABT with the highest uptake in HERA was the anti-VEGF receptor domain-fusion aflibercept, the ABM of which has a higher positive charge than any of the other Fc-fused structures. Furthermore, comparing the anti-TNF-α IgG1s revealed a higher uptake of adalimumab, which exhibits a higher Fv net charge than infliximab. Interestingly, FcRn knockdown by siRNA reduced the intracellular levels of adalimumab to a similar range as that of infliximab, suggesting that differences in cellular transport between these two IgG1s could be due to FcRn-related processes.
All findings described so far concern ABTs in the absence of their cognate Ags. When used clinically, the cognate Ag will be present and bind to the ABT in question. This masks the AIRs, adds charge intrinsic to the Abs, and also increases the avidity of the Fc:FcRn interaction after immune complex formation, thus altering two important biophysical properties; (1) overall size and (2) charge. How ABTs interact with their cognate Ags dictate their complex formation properties, which again affect their signaling properties through Fcγ receptors, biodistribution and plasma half-life82,83,84,85. For example, the anti-VEGF IgG1 bevacizumab, but not the anti-VEGF receptor domain Fc-fusion aflibercept form large, multimeric immune complexes with the VEGF dimer86. Similarly, both anti-TNF-α IgG1s form large immune complexes upon binding to Ag, while the receptor domain-Fc-fusion etanercept rather forms unstable, heterogenous 1:1 and 1:2 immune complexes with the TNF-α trimer, where the trimer may dissociate into monomers61,87,88. Our findings support this, as we found IgG1s, but not receptor domain-fusions to form large immune complexes in the presence of cognate Ag.
The size of immune complexes has been shown to predict recycling via FcRn in HMEC-1 cells, with larger immune complexes being sorted to lysosomal degradation rather than recycling tubules57. However, FcRn can also prolong the half-life of Ags by recycling and transcytosing Ag-bound IgG31,89,90, and FcRn blockade reduces the amounts of immune complexes in circulation35. Elaborating on this, we found that immune complexes of similar size displayed distinct differences in their pH-dependent binding to FcRn and cellular sorting in HERA. In general, however, Fc-multimerization increased the association and decreased the dissociation from FcRn in SPR. Furthermore, it invariably increased both intracellular uptake and recycling in HERA, but whether the increased uptake was FcRn dependent varied independently of immune complex size.
Thus, other factors must be affecting the cellular transport of Ag-bound ABTs. We found that pH modulates Ag-binding for all ABTs with a pH-dependent charge in their AIRs. Of note, our charge analyses revealed an inherent negative charge of both TNF-α and VEGF of different magnitudes. The difference in Ag charge will be amplified in solution, due to the respective trimerization and dimerization of TNF-α and VEGF, yielding a substantial negative charge of TNF-α bound ABTs compared to VEGF-bound ABTs. On the FcRn-column, we observed that these Ag-associated differences affected dissociation from the receptor for specifically TNF-α bound ABTs. As this was the case for both the large immune complexes formed by IgG1s and the smaller, less stable receptor domain-fusion complex, this must be due to charge, rather than immune complex size. VEGF-binding affected dissociation for neither anti-VEGF molecule.
In the cellular context, however, we again observed that these biochemical observations did not predict FcRn-mediated transport. Despite the fact that all anti-TNF-α ABTs were affected by Ag-binding on the column, only the IgG1s were affected in HERA. Furthermore, while Ag-binding increase the uptake of both IgG1s, the increase was FcRn-dependent for adalimumab only. In contrast, VEGF-binding drastically increased the uptake of both the IgG1 and receptor domain-fusion, but in an FcRn independent manner. However, subsequent recycling was much more efficient for the large IgG1 immune complex than the small VEGF-bound receptor domain-fusion complex, which was rather retained within cells. As the anti-VEGF IgG1 was the only ABT with negatively charged AIRs, this suggests that the combination of negative charge and Fc-multimerization had a distinct impact on cellular transport.
Interestingly, whether Ag-binding enhanced cellular accumulation of ABTs correlated with the presence of pH-dependent charge in their AIRs, as the receptor domains of the anti-TNF-α Fc-fusion etanercept was the only Fc-fused moiety without such charge. Consequently, etanercept was the only ABT to not exhibit increased uptake when bound to its Ag. This was the only noticeable charge anomaly for the receptor domains of etanercept, as they lack pH-dependent surface charge patches and possess an overall net charge similar to the Fv of the anti-TNF-α IgG1 infliximab. Thus, for this particular Fc-fusion, receptor domain-specific traits, other than large charge variations, must be the cause of reduced capacity to undergo FcRn-mediated processes. This could be due to structural flexibility of the receptor domain-domains, which could affect their orientation relative to the membrane upon binding to FcRn. Another possibility is differences in N-linked glycosylation. Both the aflibercept and the etanercept receptor domains harbor tentative N-glycosylation sites (4 and 2, respectively, predicting from the amino acid sequence N-X-S/T91), and the only such site in the IgG1s included in our studies is found in the infliximab Fv. N-glycans may affect antibody binding to both effector molecules and FcRn92. Whether such effects could explain the characteristic cellular transport of Fc-fusions, should be accounted for in future studies.
While Ag-binding affected the uptake and recycling in HERA differently for the various ABTs, the effect of Ag-binding on transcytosis was rather similar for all. Thus, how the distinct biophysical properties of both ABTs and Ags affect cellular transport clearly vary between transport pathways.
From a methodological perspective, the present findings reveal that studies of FcRn-mediated processes must carefully consider (1) the embedment the receptor in the cell membrane, evident by the lack of correlation between binding kinetics and cellular transport, and (2) the competitive pressure on the IgG-binding site on FcRn, evident by the impaired recycling efficiency of receptor domain-fusions yielding a poor competitive ability in HERA. We speculate that accounting for both these factors may allow for prediction of how the biophysical properties of IgG1 Fc-based ABTs dictate the nature and outcome of their interaction with FcRn.
While the Fab arms have evolved to grasp specific Ags, they may also modulate cellular handing and the ability to engage FcRn. Consequently, this results in a distribution of antibodies between the extracellular milieu and intracellular endosomal space, as well as between the blood, lymphatic system and mucosal tissue and surfaces. Importantly, complex formation and the biological fate of Ag-bound ABTs varies across molecular designs82,86, and others have found receptor domain-containing Fc-fusions to exhibit reduced binding to FcRn compared to IgG1s93. Our study supports this, and demonstrates that Fc-fused modalities have very distinct effects on immune complex formation and cellular handing. We show that these effects do not relate directly to their FcRn-binding capacity at acidic pH in the absence of their Ag, but rather to the biophysical differences of both ABTs and Ags, which affect the ability of ABTs to interact with FcRn in cellular membranes and be transported. In conclusion, our findings inform on the outcome of fusing different moieties to the IgG1 Fc. Such knowledge improves our understanding of antibody biology, as well as guide the design and preclinical testing of ABT candidates.
cDNA sequences encoding the HC of IgG1 Fc were designed based on its amino acid sequence as defined by the Uniprot entry IGHG1_HUMA. and synthesized in a pFuse vector with zeocin resistance by Genscript Biotech Corporation. Generation of the anti-NIP IgG1 has been previously described52,53. Both the IgG1 Fc and anti-NIP IgG1 were produced in human embryonic kidney (HEK) 293E cells, essentially as described53,56. Vectors were transiently transfected into HEK293E cells using Lipofectamine 2000 (Life Technologies) followed by harvesting and replacement of growth medium every second day for 2 weeks. Both proteins were purified on CaptureSelect (Thermo Fisher) affinity columns (anti-CH3 for IgG1 Fc and anti-CH1 for anti-NIP IgG1, respectively) prior to SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare) coupled to an Äkta FPLC instrument (GE Healthcare). Finally, monomeric fractions were upconcentrated on Amicon Ultra-15 ml columns (Millipore) with respective cut-offs at 10 K and 50 K for IgG1 Fc and anti-NIP IgG1. Purified fractions were tested by non-reduced and reduced SDS-PAGE and a two-way anti-Fc ELISA as described below. Truncated monomeric His-tagged FcRn was produced in a Baculovirus expression system as described56,94. Controls (briakinumab and anti-NIP IgG1s MST/HN and IHH) were produced as described56 (Grevys et al., manuscript in preparation). ABTs, specifically etanercept (Enbrel™), aflibercept (Eylea™), infliximab (Remicade™), adalimumab (Humira™), bevacizumab (Avastin™), eftrenonacog alfa (Alprolix™) and IVIg (Panzyga™), were acquired from the pharmacy. Etanercept, aflibercept, infliximab, adalimumab, bevacizumab and eftrenonacog alfa were buffer-exchanged into sterile phosphate-buffered saline (PBS) and run on SEC as described above prior to starting experiments.
The HEK293E cell line (ATCC) was cultured in RPMI-1640 GlutaMAX-containing medium (Sigma-Aldrich) supplemented with 10% fetal calf serum (FCS) 25 U/ml penicillin (Sigma-Aldrich) and 25 μg/ml streptomycin (Sigma-Aldrich). The MDCK cell line stably expressing hFcRn (MDCK-hFcRn) was generated by Drs. Jens Fischer and Alex Haas (Roche Pharma Research and Early Development), and cultured in cultured in DMEM with 15% FCS, 25 U/ml penicillin and 25 μg/ml streptomycin, as well as 100 μg/ml G418 (Sigma-Aldrich) to maintain expression of FcRn. HMEC-1 cells stably expressing HA-FcRn-EGFP (HMEC-1-FcRn57) were cultured in MCDB131 medium (Gibco) supplemented with 2 mM L-glutamine, 25 μg/ml streptomycin, 25 U/ml penicillin, 10% FCS, 10 ng/ml mouse epidermal growth factor (BD Biosciences) and 1 μg/ml hydrocortisone (Sigma-Aldrich), and 100 μg/ml G418 and 50 μg/ml blasticidine (InvivoGen) to maintain FcRn expression. Percentage of HMEC-1 cells expressing HA-FcRn-EGFP in culture was above 85%, which was verified by visualizing EGFP-positive live cells, stained with the amine-reactive dye ViViD (Invitrogen), in combination with a biotinylated anti-FcRn mAb (ADM3195) and allophycocyanin-conjugated streptavidin (PhycoLink) using a BD LSRFortessa.
ELISA was performed to quantify protein levels in plasma samples, cell lysates and medium samples from cellular experiments. All quantification experiments were performed with the molecule to be analyzed as its own standard. Briefly, in a two-way anti-Fc ELISA, polyclonal anti-Fc (Sigma-Aldrich) from goat was diluted 1:5000 in PBS and coated by adding a volume of 100 μl in ELISA wells and incubating at 4 °C overnight (ON). Next, all wells were blocked by adding 250 μl of PBS supplemented with 4% skimmed milk (M) and incubating on a tabletop shaker for 1 h at room-temperature (RT). Plates were washed four times with 200 μl PBS containing 0.05% Tween20 (T; PBST) between all subsequent steps. Plasma and cellular samples were then added in 100 μl at a final dilution ranging from 1:50 to 1:400 (plasma) and 1 to 16 (cellular samples) in PBSTM. Following a 1-h incubation at RT with shaking, a polyclonal anti-human Fc from goat conjugated to alkaline phosphatase (Sigma-Aldrich) was diluted 1:5000 in PBSTM and added at a final volume of 100 μl per well. After a final 1-h incubation at RT with shaking, 100 μl of 10 μg/ml p-nitrophenyl-phosphate substrate (Sigma-Aldrich) dissolved in diethanolamine solution was added to all wells to develop the ELISA. A Sunrise spectrophotometer (Tecan) was used to measure absorbance at 405 nm. For capture of ABTs on cognate Ag, 500 ng/ml of cognate Ags (VEGF-165 (Sino Biologics), TNF-α (Peprotech) or NIP-conjugated bovine serum albumin (BSA-NIP25)) were coated in a final volume of 100 μl. Subsequent steps in Ag-binding ELISA were performed as described for the two-way anti-Fc ELISA.
FcRn KO mice (B6.129 × 1-Fcgrt tm1Dcr/Dcr), Tg32 hemizygous hFcRn transgenic mice (B6.Cg-Fcgrt tm1Dcr Tg(FCGRT)32Dcr/DcrJ) and Tg32 homozygous hFcRn transgenic albumin knock-out mice (B6.Cg-Albem12Mvw Fcgrttm1Dcr Tg(FCGRT)32DcR/MvwJ) received 0.8 mg/kg IgG1 Fc or 2.6 mg/kg anti-NIP IgG1. In a separate experiment, homozygous Tg32 mice also received 1.7 mg/kg aflibercept, 0.8 mg/kg IgG1 Fc,1.8 mg/kg etanercept and 1.7 mg/kg eftrenonacog alfa. All experiments were done with a mix of male and female aged 8–10 weeks, with a weight of 20–30 g/mouse and five mice per group. For experiments with IgG1 Fc and anti-NIP IgG1, 25 μl blood samples were obtained from the retro-orbital sinus vein at either 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, 6 and 7 days after injection (FcRn KO mice), or 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, 7, 10, 12, 16, 19, 23 and 30 days after injection (hemizygote Tg32 mice), or 1, 2, 3, 5, 7, 10, 12, 12, 16, 19, 23, 30 and 37 days after injection (homozygote Tg32 mice). In the separate experiment where homozygous Tg32 mice were dosed with IgG1 Fc, aflibercept or etanercept, blood sampling was performed as previously described at 1, 2, 3, 4, 5, 7, 10, 12, 16, 19 and 23 days after injection. All blood samples were mixed with 1 μl % K3-EDTA and kept on ice up until centrifugation at 4 °C for 5 min at 17,000 × g. Isolated plasma was diluted 1:10 in 50% glycerol/PBS and stored at −20 °C until analysis. All animal studies were performed at The Jackson Laboratory (JAX Service, Bar Harbor, ME), in accordance with guidelines and regulations approved by the Animal Care and Use Committee at The Jackson Laboratory. Protein plasma concentrations were quantified by the two-way anti-Fc ELISA as described above.
Plasma levels of the Fc-containing molecules were determined by nonlinear regression in GraphPad Prism (version 8.3.1, GraphPad Software LLC) using a log(agonist) vs. response equation for variable slopes. Levels at the end of the presumed the α-phase (i.e., after 24 h) were set to 100% and plasma concentrations calculated as a percentage relative to that level. A nonlinear fit of the resulting values to a semilogarithmic model was done to visualize compound elimination. β-phase half-life was calculated in Microsoft Excel version 16.16.19 (200210) by the equation t1/2 = log 0.5(logAx/A0)*t, where A0 is the amount entering the β-phase, and Ax is the amount calculated at the given time (t). For further pharmacokinetic parameters, the molecular concentrations determined from ELISA were fitted to a non-compartmental pharmacokinetic model using the gPKDsim add-on for MatLab55.
Preincubation of ABTs with cognate Ags
For all experiments involving preincubation of ABT with cognate Ags, preincubation was done by adding the specified ratios of Ag and ABT in a volume of 20 μl of the appropriate buffers, mixing thoroughly and incubating at RT for 20 min prior to experiment initiation. Where injection of such samples is specified, the injection, preincubation and running buffers were all the same.
Analytical size-exclusion chromatography
Analytical SEC was performed using a Superdex 75 10/300 column coupled to an Äkta FPLC instrument (Cytvia). Injections were performed in either sterile PBS (Gibco) for pH 7.4 studies or PBS adjusted to pH 5.5 with 1 M MES for cell culture (Sigma). In total, 1 μg/ml of the ABTs was injected in a total volume of 20 μl. For studies of complex formation, 1 μg/ml of ABTs were preincubated with the specified molar ratios of their cognate Ags in an end volume of 20 μl of either PBS pH 7.4 or 5.5 for 20 min at RT prior to injection.
Analytical FcRn chromatography
Analytical FcRn affinity chromatography was performed using an Äkta Avant25 instrument (Cytvia), as described56. In total, 77 µl of a 1 mg/ml protein solution was injected on a column with FcRn-coupled resin in a pH 5.5 buffer (20 mM MES, 140 mM NaCl), and eluted through a lineal pH gradient obtained by gradual increase of a pH 8.8 buffer (20 mM Tris-HCl, 140 mM NaCl) throughout 110 min. Accurate pH elution values were determined by use of a pH monitor (Cytvia). For IC studies, 1 mg/ml of the ABTs were preincubated with their cognate Ags as described above, here in the 20 mM MES, 140 mM NaCl buffer, prior to dilution into the specified injection volume and column application.
Surface plasmon resonance
SPR experiments were performed using a Biacore T200 instrument (Cytvia). Binding of ABTs to cognate Ags was studied by immobilizing ~300 resonance units (RU) of either VEGF-165 or TNF-α on CM5 sensor chips using the manufacturer’s amine coupling kit. In total, 100 nM of each ABT was diluted in a running buffer of either pH 5.5 (67 mM phosphate, 0.15 M NaCl, 0.005% Tween20) or adjusted to pH 7.4 with 1 M NaOH, and injected at a flow rate of 20 μl/min. Also, ABTs were preincubated for 20 min at RT in a volume of 20 μl with 200 nM monomeric, His-tagged FcRn in the pH 5.5 running buffer, followed by injection over the respective cognate Ags. Ten mM NaOH at 20 μl/min was used for regeneration. All individual injections were normalized to baseline, all sensorgrams zero-adjusted in the BIAevaluation software version 4.1 (GE Healthcare), and all shown data have been subjected to blank subtraction of the response on a flow cell from the same chip.
Binding kinetics of the monomeric ABTs to FcRn were determined by immobilizing the ABTs at ~400 RU on CM5 chips with the manufacturer’s amine coupling kit. Serial dilutions of monomeric His-tagged FcRn (2000-1.95 nM) were prepared and injected in the pH 5.5 running buffer at 50 μl/min. Regeneration was done using pH 7.4 HBS-P + (0.15 M NaCl, 0.1 M HEPES, 0.005% surfactant P20) at 30 μl/min with contact and stabilization periods of 30 s.
Binding of Ag-bound ABTs to FcRn was determined by immobilizing site-specifically biotinylated FcRn (Immunitrack) on SA-chips at ~100 RU. ABTs (15.62 nM) were injected either alone or following preincubation with cognate Ags as previously specified in the pH 5.5 running buffer at a flowrate of 50 μl/min. Regeneration was done with HBS-P + pH 7.4.
For HERA experiments, 3.75 × 104 HMEC-1-FcRn cells were seeded in a volume of 250 μl in Costar 48-well plates in the culturing medium 20–24 h prior to experiment initiation. Three wells were seeded per experimental parameter. Following visual inspection to confirm characteristic morphology and sufficient confluency, the medium was removed from all wells and the cells washed thrice in 250 μl RT Hank’s balanced salt solution (HBSS, Gibco). After washing, the cells were starved for 1 h in 250 μl RT HBSS.
For studies of the ABTs in the presence of cognate Ag, ABTs were next prepared at a final concentration of 800 nM in RT HBSS either alone or following preincubation in 25 μl for 20 min at RT with two-fold molar excess of their cognate Ags, then added to cells at a final volume of 125 μl in technical triplicates. For studies of anti-NIP IgG1 and the Fc fragment, both molecules were prepared as 800 nM samples and added to cells as specified for ABTs. After a 3-h incubation, the samples were removed and the cells washed three times in 250 μl ice-cold HBSS. At this time, uptake plates were frozen at −80 °C following aspiration of washing medium, while recycling plates received 220 μl RT serum-free growth medium supplemented with MEM non-essential amino acids. After another 3-h incubation, recycling samples were harvested and washing with ice-cold HBSS repeated. Resulting residual plates were frozen and stored at −80 °C to the day of analysis.
For competition studies, cells were incubated for 1 h in 125 μl HBSS supplemented with 0.5% IgG-depleted FCS (Gibco) either with or without 7.5 mg/ml (unless elsewise specified) IVIg (Octapharma), depending on the specified competition setting (i.e., preloading of cells). Following preloading, cells were washed twice in 250 μl ice-cold HBSS, pH 7.4, before adding samples at a concentration of 400 nM in a final volume of 125 μl. Where specified, samples were prepared in HBSS adjusted to pH 6.0 by adding 7 mM MES buffer (Sigma-Aldrich). For studies of the capacity of Fc-containing molecules to reduce recycling of anti-NIP IgG1, this step was performed with a titration series with final concentrations 6400-1 nM of each ABT were prepared with 400 nM anti-NIP IgG1 in pH 6.0 HBSS. Storage of cell plates, incubation, washing, harvesting of recycling samples was done as described above.
At the day of analysis, recycling samples were thawed at RT, and frozen cells were lysed by adding 220 μl RIPA buffer (Thermo Fisher) supplemented with cOmplete protease inhibitor cocktail (Roche) and incubating on ice on a shaker for 10 min. Cellular debris was removed by 5 min centrifugation at 10,000 × g and proteins quantified by ELISA as described above. Samples were analyzed by ELISA as described above. Specifically, samples for quantification of the IgG1 Fc fragment and ABTs preincubated with cognate Ags were analyzed by the two-way anti-Fc ELISA. Samples from experiments involving preloading cells with IVIg were analyzed by Ag-binding ELISAs, with the exception of quantification of total amount of IgG in cells, which was done by the two-way anti-Fc ELISA.
siRNA knockdown of FcRn expression in HERA
HERA in siRNA-treated cells was performed as described56. Briefly, 3.75 × 104 HMEC-1-FcRn cells were seeded in a total volume of 500 μl in 24-well Costar plates in the culturing medium 20–24 h prior to siRNA transfection. Transfection with mixtures of FcRn-HC-specific siRNA (sc-45643, Santa Cruz Biotechnology Inc) and control siRNAs (sc-37007) was performed as described by the manufacturer. After transfection, cells were incubated for 48 h before performing the uptake phase of HERA as described above, with 400 nM end concentration of the specified analytes in a volume of 250 μl being added to cells followed by an incubation of either 0.5 or 2 h.
In total, 1.12 cm2 diameter transwell filters with a 400 nm pore size and collagen-coated polytetrafluoroethylene membranes (Corning Costar) were incubated ON in the cell incubator with complete growth medium prior to seeding of 1–1.25 × 106 MDCK-FcRn cells/well. Cells were incubated for 18–24 h to reach confluence and a transepithelial electrical resistance of 7–800 Ω cm2. Next, both chambers were washed two times with 500 μl RT HBSS prior to starving cells for 1 h in 500 μl RT HBSS. Next, 200 nM analyte was added to the top filter in an end volume of 200 μl. Cells were then incubated for 4 h in the cell incubator before harvesting samples from the bottom chamber. Protein levels were determined by the two-way anti-Fc ELISA as described above. For ABT:Ag studies, ABTs were preincubated with their cognate Ags as described above before being diluted to an end concentration of 200 nM and added to cells.
Protein sequence alignment and sequence numbering
Amino acid sequences were aligned using ClustalOmega and Jalview 2.10.5. Where specified, amino acid residue positions refer to standard EU numbering as defined by IGHG1_HUMA, Uniprot accession #P01857.
Visualization of crystal structures and surface charge
Structural and surface charge analyses were done based on solved X-ray crystallographic structures retrieved from the PDB of bevacizumab (PDB ID: 1BJ1), adalimumab (PDB ID: 3WD5), infliximab (PDB ID: 4G3Y), and etanercept (PDB ID: 3ALQ). For aflibercept, a homology model was generated by using the structures of VEGFR1 domain 2 (PDB ID: 1QTY) and VEGFR2 domain 3 (PDB ID: 3V2A). The connecting linker and missing loops in aflibercept were modeled using Rosetta Remodel96. Ag structures were isolated from 3ALQ (chain A + B + C) and 1BJ1 (chain V + W).
To visualize surface charge, pqr files were generated using the pdb2pqr web server version97 with default settings. Electrostatic potentials at pH 5.5 and 7.4 were calculated as indicated, by use of PARSE force field and APBS version 3.098. The isopotential surfaces were visualized at ±3 kT/e (ABMs) or ±5kT/e (Ags) using pymol version 2.4.0. AIRs were determined as CDR loops by Chothia for Fvs and as residues with at least one atom within a 5 Å radius of an Ag atom using pymol for receptor domains.
Calculation of net protein charge
Sequence-dependent net charge at different pH values were calculated using the Emboss iep calculator (http://www.bioinformatics.nl/cgi-bin/emboss/iep?_pref_hide_optional=0). All cysteines were assumed to form disulfide bridges. Whole ABMs were defined as the combined HC and LC sequence for Fvs and as the whole receptor domain sequence for Fc-fusions, and were assumed to have one N-terminal residue per polypeptide chain. Remaining residues were defined as the whole ABMs after removing AIRs, and were assumed to have one N-terminal residue. AIRs were not assumed to have any terminal residues.
Statistics and reproducibility
The data is presented as mean ± SD. Unpaired Student’s t test was used to compare pairs of experimental parallels where specified. t-Tests were performed using Prism 8 (GraphPad, San Diego, CA). Two-tailed p values ≤0.05 were considered as statistically significant. Sample sizes were pre-determined in line with previous work with related methods, and are in all cases stated in figure Legends. For cellular experiments where statistics were applied, the sample size was generally three biological replicates, with three technical triplicates within each experiment. For animal experiments, sample size was set to 5 individual animals.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Raw data underlying data shown in main figures is found in the Supplementary Data, and are available from the corresponding author upon reasonable request.
The following software and repositories were used for data collection: Microsoft Excel version 16.16.19 (200210), GraphPad Prism (version 8.3.1, GraphPad Software LLC), BIAevaluation software version 4.1, MatLab R2021a, PyMOL version 2.4.2, Emboss iep calculator (https://www.bioinformatics.nl/cgi-bin/emboss/iep?_pref_hide_optional=0), www.drugbank.com, Clustal Omega version 1.2.4, Rosetta Remodel (doi: 10.1371/journal.pone.0024109), PDB2PQR (doi: 10.1093/nar/gkh381), The following software was used for data analysis and graphical presentations: Microsoft Excel version 16.16.19 (200210), GraphPad Prism (version 8.3.1, GraphPad Software LLC), BIAevaluation software version 4.1, MatLab R2021a, PyMOL version 2.4.2, Jalview 2.10.5, www.biorender.com. Code for generation of the aflibercept model by the use of Rosetta is available in a Github repository, as described in the accompanying Code and Software Submission Checklist, and may be accessed at the following link upon reasonable request: https://github.com/rahelf/aflibercept_model.git.
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We thank Rituparna Samanta, PhD, at the Department of Chemical Engineering at Johns Hopkins University, Baltimore, MD, USA, for helpful discussions regarding particle complex formation, and Dr. Wayne I. Lencer at the Boston Children’s Hospital, Harvard Medical School and Harvard Digestive Diseases Center, USA, for the HMEC-1-HA-FcRn-EGFP cell line. T.T.G. was supported by internal funding from the Division of Head, Neck and Reconstructive Surgery at Oslo University Hospital, and in part by the Dr. Jon S Larsens Foundation, the Futura Fund for Scientific Medical Research, the Aase Bye and Trygve J.B. Hoff Ophthalmological Fund as well as the Norwegian Association of Blind and Partially Sighted. J.T.A. was supported by the South-Eastern Norway Regional Health Authority (Grant nrs. 2018052; 2019084), the Research Council of Norway (Grant nrs. 274993; 287927; 314909).
M.C.M. has been a member of advisory boards at Bayer, Novartis, Roche and Allergan and has received lecture fees from Bayer and Roche. Ø.K.J. has been member of Bayer, Allergan and Roche advisory boards and has received lecture fees from Bayer and Allergan. The remaining authors declare no competing interests.
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Gjølberg, T.T., Frick, R., Mester, S. et al. Biophysical differences in IgG1 Fc-based therapeutics relate to their cellular handling, interaction with FcRn and plasma half-life. Commun Biol 5, 832 (2022). https://doi.org/10.1038/s42003-022-03787-x