Abstract
Red blood cells (RBCs) capability to deliver oxygen (O2) has been routinely measured by P50. Although this defines the ability of RBCs to carry O2 under equilibrium states, it cannot determine the efficacy of O2 delivery in dynamic blood flow. Here, we developed a microfluidic analytical platform (MAP) that isolates single RBCs for assessing transient changes in their O2 release rate. We found that in vivo (biological) and in vitro (blood storage) aging of RBC could lead to an increase in the O2 release rate, despite a decrease in P50. Rejuvenation of stored RBCs (Day 42), though increased the P50, failed to restore the O2 release rate to basal level (Day 0). The temporal dimension provided at the single-cell level by MAP could shed new insights into the dynamics of O2 delivery in both physiological and pathological conditions.
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Introduction
Oxygen (O2) is required for maintaining various vital cellular functions and its supply to tissues is maintained by the cardiovascular system that ramifies throughout every organ in the body. Deprivation of O2 leads to tissue hypoxia, ultimately inducing cell dysfunction and apoptosis. Delivery of O2 is facilitated by red blood cells (RBCs) which constitute the majority of blood cells. Owing to its densely packed hemoglobin (Hb), RBCs can bind with O2 and release it on demand under deoxygenated conditions. Yet, the ability of RBCs to deliver O2 has been routinely characterized solely by their HbO2 affinity (P50), defined as the partial pressure of O2 (PO2) required to saturate Hb to 50% in a thermodynamic equilibrium process1. This metric, however, neglects the dynamic release rate of O2 from RBCs, and hence may be insufficient to assess the efficacy of RBC O2 delivery in the microcirculation. In contrast, assessing the temporal aspect of O2 delivery using the dynamic O2 release rate of RBCs would afford a more direct measure of the O2 transport by RBCs2.
O2 transport in the microvasculature typically occurs within a time frame of 4–7 s3. During the unloading process, O2 diffuses across the RBC membrane after dissociating from Hb. It has been shown that the internal cytoplasmic resistance of the RBC to O2 transport is not negligible4,5. Consequently, the time required for an RBC to unload O2 is dependent on both this internal resistance and the external milieu. Moreover, O2 uptake and release in RBCs are known to depend on their morphological and physiological properties6. Since RBCs exist as a non-homogenous entity of various biological ages with different biophysical and biochemical properties7,8,9, how these variations ultimately affect the O2 release rate remains unclear. Furthermore, a single-cell measurement is particularly important for RBC studies since there is vast cellular heterogeneity in the RBC population, which could result in the masking of functionally important subpopulations. Hence, profiling individual cells is essential to provide a representation of cellular level events in lieu of stochastic averages from bulk measurements10. Our motivation for this study is echoed by a recent study that investigated alterations in the O2 release rate of RBCs with varying cytoplasmic diffusivity11. Specifically, they have shown that an increase in pathlength or tortuosity of RBCs under engineered and diseased conditions can lead to a decrease in O2 transport. However, their measurements failed to isolate single cells from intercellular diffusion, unlike the current study.
Here, we use a diffusion parameter, D50, to better explain how fast each RBC can diffuse O2 to the surrounding tissues. For the isolation of a single RBC, we developed a microfluidic analytical platform (MAP) to examine the O2 diffusion from each single RBC, from which we derived the population variability of the O2 release dynamics. Using this MAP, we examined the effect of in vivo aging on the O2 release rate of RBCs and found that biologically older RBCs exhibited higher O2 release rates. We also demonstrated the effect of RBC storage lesion on the O2 release rate, which revealed a faster O2 release rate in stored RBCs than fresh cells. Moreover, we confirmed that the P50 value alone does not substantiate how fast RBCs can diffuse O2 even at the bulk level.
Results
MAP design and validation
The MAP was designed with an array of microwells for isolating single RBCs in the main microchannel. The microwells were then deoxygenated by nitrogen (N2) flowing in the parallel gas microchannel, which is separated from the microwells by 25-µm polydimethylsiloxane (PDMS) layer (Fig. 1a). Using fluorescence microscopy (Fig. 1b), we determined the rate of RBC deoxygenation from the temporal variations of fluorescence intensity in the microwells (Fig. 1c). As the presence of RBCs in the microwells provided an additional source of O2, higher rates of O2 release by the RBCs would thus reduce the rate of fluorescence decay in the microwells. This fluorescence quenching approach allows for the direct measurement of local O2 concentration. To validate the MAP, we established the decay rate of the O2 depletion in the microwells without RBCs, which corroborated the decay profile obtained from our computational simulation (R2 = 0.97) (Supplementary Fig. 2). To quantitatively assess the O2 release rate from single RBCs, we first fitted the experimental data using nonlinear regression, \({\rm{PO}}_2 = Ae^{ - Kt}\), where A is the initial PO2 in the microwell (155.3 mmHg), K denotes the decay constant, and t represents time. The obtained decay constant was then used to compute the time taken for the O2 level in the microwell to decrease by 50% from the initial equilibrium concentration (\({\rm{D}}50 = {\rm{ln}}(2){\rm{K}}^{ - 1}\)). Therefore, a higher D50 value indicates a faster O2 release rate from the RBC. Our results revealed that microwells with single RBCs showed a significantly larger D50 (1.17 ± 0.52 s) than those without RBCs (0.80 ± 0.29 s) (Fig. 1d).
In vivo aging enhances the O2 release rate of RBCs
To investigate the effects of in vivo aging on the O2 release rate of RBCs (Fig. 2), freshly drawn RBCs from healthy donors (n = 5) were density-fractionated into two groups: younger (top 20%) and older (bottom 20%). Older RBCs (41.3 ± 4.3 g/dL) had a significantly higher mean corpuscular hemoglobin concentration (MCHC) than younger RBCs (35.9 ± 1.7 g/dL). Absolute 2,3-DPG was also found to be substantially lower in older RBCs (1.76 ± 0.26 mM) than that in younger RBCs (2.80 ± 0.81 mM). As a result, normalized 2,3-DPG of older RBCs (4.32 ± 0.9 µmol/g Hb) was correspondingly lower than younger RBCs (7.78 ± 2.24 µmol/g Hb). Consequentially, the P50 of older RBCs (29.9 ± 1.0 mmHg) was lower than younger RBCs (32.0 ± 1.7 mmHg). The process of deoxygenation induces a conformational change in Hb from the relaxed (R) to the tense (T) structure, where Hb in the R state has a higher affinity for O2 than that in the T state. The 2,3-DPG binds to the central cavity of the deoxygenated Hb and anchors the molecular configuration of Hb in the T state. Hence, a lower 2,3-DPG level, as observed in the older RBCs, increases the Hb affinity for O2.
Interestingly, despite the increase in O2 affinity as determined by the P50 level (Fig. 2d), the diffusion rate (D50) of older RBCs (1.17 ± 0.32 s) was significantly higher than younger RBCs (1.03 ± 0.27 s) (Fig. 2e), implying a faster O2 release rate. D50 is governed by O2 diffusion through the cytoplasm and across the cell membrane. MCHC increases with RBC age through the reduction in cell volume via microvesiculation12. This results in a substantial tortuosity to the movement of solutes in the cytoplasm due to molecular crowding13. However, this is countered by a reduction in the diffusion path-length2,14. We observed an overall increase in D50 with RBC age, which implies the dominance of the reduced path-length over cytoplasmic diffusivity in regulating O2 diffusion. In probing the O2 release rates at the single-cell level, we were able to obtain and compare the distributions of D50 between older and younger RBCs (Fig. 2f), which showed that the full width at half maximum of the D50 distribution in older RBCs (0.75) was also larger than younger RBCs (0.64). In addition, the skewness for the D50 distributions of younger and older RBCs was determined to be 0.17 and 0.48, respectively, indicating a stronger deviation from normality toward higher D50 values in older RBCs.
Blood storage enhances the O2 release rate of RBCs
To examine the effect of in vitro aging on the O2 release rate of RBCs, fresh RBCs were stored for 42 days following the standard blood banking protocol. Figure 3 shows changes in the O2 affinity-related factors of the stored RBCs. No significant alteration in the MCHC was observed during the storage duration. In contrast, absolute (Day 0: 2.77 ± 0.52 mM, Day 7: 1.10 ± 0.32 mM) and normalized 2,3-DPG concentrations (Day 0: 8.61 ± 1.51 µmol/g Hb, Day 7: 3.49 ± 0.99 µmol/g Hb) dropped drastically by Day 7 and came close to depletion on Days 28 and 42. Consequently, P50 of stored RBCs decreased significantly with storage duration, indicating an increase in O2 affinity (Day 0: 33.2 ± 0.87 mmHg, Day 42: 24.9 ± 0.16 mmHg) (Fig. 3d). Detailed experimental results can be found in Supplementary Table 1.
As opposed to the decrease in the P50, the D50 of stored RBCs (Fig. 4) was significantly higher than that of the fresh RBCs (Day 0: 0.87 ± 0.30 s, Day 42: 1.08 ± 0.26 s). The D50 distribution at Day 0 assumed a Gaussian profile. However, the distribution deviated from Gaussian at Day 1 and tended toward a bimodal distribution by Day 14; this bi-modal distribution seemingly reverted to normality again by Day 42. Concomitantly, the distribution was initially right-shifted toward higher D50 but became left-shifted from Day 14. The full width half maximum (FWHM) for each of the storage durations was also calculated (Supplementary Fig. 3). We found that Day 14 (1.32) had an ∼86% increase in FWHM as compared to Day 0 (0.71). The FWHM then decreased after Day 14 and by Day 42, the FWHM reduced to ∼13% less than Day 0 (Day 28: 1.14, Day 42: 0.62). Considering these observations, we hypothesized that different RBC subpopulations undergo differential rates of hemolysis during storage, which would account for the emergence of bimodal distributions and gradual left-shift with longer storage durations. To confirm this, we measured the hemolysis level of density-fractionated stored RBCs (for 42 days) in a separate experiment. Notably, older (denser) RBCs (1.81 ± 0.36%) showed a ~35% higher hemolysis than younger RBCs (1.34 ± 0.39%) after 42 days.
Rejuvenation of 2,3-DPG does not improve the O2 release rate
To determine the effect of 2,3-DPG rejuvenation on the O2 release rate of stored RBCs, Day 42 RBCs were incubated in 10 mM inosine and pyruvate solutions. The rejuvenation process increased the MCHC of the stored RBCs by ~5% (Day 42: 30.8 ± 1.1 g/dL, Rejuvenated RBCs: 32.4 ± 1.1 g/dL) (Fig. 5a). As expected, absolute and normalized 2,3-DPG concentrations of Day 42 RBCs were restored to the levels similar to those of fresh RBCs (Day 0: 2.77 ± 0.52 mM, Day 42: 0.06 ± 0.06 mM, Rejuvenated RBCs: 2.96 ± 0.51 mM for absolute values; Day 0: 8.61 ± 1.51 µmol/g Hb, Day 42: 0.20 ± 0.18 µmol/g Hb, Rejuvenated RBCs: 9.16 ± 1.63 µmol/g Hb for normalized values) (Fig. 5b, c). Rejuvenation of 2,3-DPG led to a significant increase (~20%) in the P50 of stored RBCs (Day 42: 24.9 ± 0.16 mmHg, Rejuvenated RBCs: 29.2 ± 0.99 mmHg) (Fig. 5d), although it was still significantly lower than the fresh RBCs (Day 0: 33.2 ± 0.87 mmHg). On the other hand, the measured D50 values (Fig. 5e) before and after rejuvenation were not significantly different (Day 42: 1.08 ± 0.26 s, Rejuvenated RBCs: 0.98 ± 0.28 s).
Discussion
The fundamental role of RBCs is to deliver O2 to the tissues to sustain key metabolic processes that are required to maintain life. Hence, determining the efficacy of RBC O2 delivery is crucial in explaining a wide range of cellular phenomena in both physiological and pathological conditions. Many previous studies have emphasized the significance of the temporal aspect of O2 delivery15,16. Specifically, a decrease in RBC residence time or higher RBC flow velocity in the microcirculation could lead to tissue hypoxia due to reduced O2 release from the RBCs17.
The cellular heterogeneity that exists within the RBCs population demands single-cell analyses, which can provide new insights into RBCs O2 delivery functionally18,19. Di Caprio et al.18 developed a microfluidic platform to measure single-cell O2 saturation under continuous flow and demonstrated single-cell variability in the measured population. While the previous study provided steady-state measurements in terms of P50, this study focused on the measurement of dynamic temporal changes in isolated single RBCs. Recently, Richardson et al.11 also determined the O2 release rate by loading O2-sensitive fluorescent dyes into RBCs and discussed the relative contributions of various cellular properties to the overall release dynamics. In contrast, the present study localized the O2-sensitive fluorescent dye in the suspending medium to avoid disrupting the native state of the RBCs. Compared with the previous studies, a distinctive feature of our MAP is the array of microwells, which was designed to limit the diffusion to only between the suspending medium and the isolated single RBCs. Thus, the microwells effectively allow us to obtain the diffusion kinetics of the O2 unloading without interference from intercellular diffusion, along with the diffusion of the ambient gases into the suspending medium.
O2 diffusion in RBCs
O2 uptake and release by RBCs involve complex molecular processes that occur over a span of length and time scales. RBCs uptake O2 in the alveoli in the lungs, within which O2 diffuses through blood plasma and the RBC cell membrane to reach the intracellular Hb. Within the RBCs, Hb-facilitated diffusion of O2 exhibits a biphasic trend with Hb concentration20. Moreover, increasing the amount of Hb molecules is likely to impose a substantial tortuosity to the movement of solutes in the cytoplasm2. This is supported by a previous study11 that showed a decrease in O2 transport rate across the cytoplasm due to an increase in MCHC. Nonetheless, our experimental results showed that older RBCs exhibited significantly higher O2 release rates (higher D50) than their younger counterparts. This suggests that within the physiological range of MCHC (i.e., younger: 35.9 ± 1.7 g/dl; older: 41.3 ± 4.3 g/dL), the concentration of Hb does not have a dominant effect on the O2 diffusion rate from RBCs.
During the aging process, whether in vivo or in vitro, RBCs experience a decline in the activities of vital metabolic enzymes and altered redox metabolism, leading to reduced ATP production21, oxidative damage to Band 322 and membrane vesiculation23,24. Consequently, older RBCs experience a decrease in mean cell volume (MCV)9,25, which increases the MCHC, while reducing the path-length and the time required for O2 to diffuse across the cytoplasm to the inner side of cell membrane2, thus enhancing the rate of O2 release from RBCs11. The reductions in MCV and total surface area of the membrane in older RBCs could also lead to a decrease in the amount of membrane cholesterol26,27,28, which was previously shown to modulate O2 permeability in RBC membrane29,30,31,32,33. Therefore, these factors could cumulatively contribute to the elevation of D50 observed in older RBCs. While Richardson et al.11 demonstrated the effects of isolated changes in RBCs, our study suggests that there is a myriad of factors within the scheme of O2 diffusion of single RBCs. Concomitantly, diffusion of NO and CO2 could also be modulated through altered deoxygenation conditions34,35 and compromised Band 336,37 as a consequence of the aging process. However, the effect of the two gases on our O2 measurements would be negligible since the RBCs are fully saturated with O2 and the NO produced by the NOS within the RBCs would be insignificant.
2,3-DPG
2,3-DPG is an intermediary metabolite in the Embden–Meyerhof glycolytic pathway that binds specifically to the central cavity of deoxyhemoglobin. This stabilizes the tense state of Hb, which reduces its O2 affinity as confirmed in our results (Figs. 2d and 3d)38. RBCs have a typical lifespan of 100–120 days during which they are constantly exposed to various insults from the external environment, leading to cumulative changes in their physical and chemical properties39. RBC senescence is associated with the exponential decline in glycolytic enzymes with RBC age, leading to reduced glycolysis and thus intracellular 2,3-DPG40. During in vitro blood storage, a similar decrease in glycolysis is attributed to the accumulation of lactic acid that lowers the pH of the storage medium41. While our results confirm that both in vivo (biological) and in vitro (storage), aging processes decrease the 2,3-DPG concentration and P50 of RBCs, we found no decline in their actual O2 diffusion rate. Interestingly, in both older and stored RBCs, D50 was significantly larger (O2 diffusion was faster) than in the younger (Fig. 2e) and fresh RBCs (Fig. 4), respectively. This suggests that 2,3-DPG does not play an important role in the regulation of O2 release from RBCs. While the decrease in 2,3-DPG concentration in aged RBCs enhances the Hb affinity for O2 (or reduces the P50 level), the release of O2 from Hb represents a subset of the whole O2 diffusion process. Hence, there might be other dominant factors that could lead to an increase in the O2 diffusion rate of aged RBC as determined by D50, which better reflects the totality of the O2 diffusion process. Moreover, the importance of 2,3-DPG on the whole scheme of O2 delivery remains unclear; a previous in vivo study demonstrated that the depletion of 2,3-DPG only had minor effects on the O2 reserve and O2 extraction42. Therefore, the use of both D50 (dynamic factor) and P50 (static factor) would provide a more comprehensive assessment of the O2 delivery capability of RBCs.
Higher vs. lower D50
Oxygenated RBCs are constantly exposed to a large O2 gradient across the tissues before returning to the pulmonary circulation. Thus, it is likely that the intrinsic variability and distribution of D50 in RBCs are favorable in contributing to uniform O2 supply in the highly heterogeneous vascular networks. There exist various compensatory mechanisms in the cardiovascular system for regulating O2 delivery. For instance, a hypoxic environment could elicit a considerable increase in the cardiac output43. However, these mechanisms may be undermined under pathological conditions. Hence, RBCs with lower D50 could be better adapted to transport O2 to distal regions. We speculate that excessive variability in the distribution of D50, as shown with our blood storage study, may reduce the overall population O2 transport efficacy due to the detrimental contributions of the poorer functioning cells and may be explored as a potential indicator of hematological disorders. RBC distribution width (RDW), which measures the coefficient of variation of RBC size, has been associated with various deleterious clinical conditions such as higher mortality in older adults44, critical illnesses45, and increased odds of prevalent dementia46. Drawing a parallel between D50 and RDW, any major deviation from homeostatic distribution could have undesirable physiological consequences.
Implication on the efficacy of blood transfusion
Transfusion of RBCs has been a stalwart therapeutic intervention for the treatment of acute and chronic anemia47,48. Its ultimate goal is to restore the tissue oxygenation level to maintain aerobic metabolism49. Currently, there is no consensus on the efficacy of stored RBC transfusion in restoring tissue oxygenation50,51,52. In this study, we found that the O2 diffusion rate of RBCs (D50) was enhanced with blood storage. RBCs undergo a myriad of biophysical and biochemical changes during storage, collectively defined as the storage lesion. Our P50 results show an increase in O2 affinity with storage duration that has mostly been interpreted as a potentially negative outcome. In contrast, the increase of D50 in stored RBCs suggests the apparent benefit of faster O2 supply to the tissues. RBC storage has been shown to increase the MCV of RBCs in some studies53,54,55,56,57,58, albeit marginally (<5%) especially when stored at 4 °C. Yet other studies showed no appreciable changes with storage59,60,61. These suggest that MCV changes with RBC storage have minimal impact on the O2 diffusion dynamics unlike in biological aging. Instead, we surmise that increased membrane permeability caused by oxidation and precipitation of membrane proteins and cellular dehydration (resulting from calcium influx and potassium efflux)62,63 may have contributed to the enhanced diffusion of solutes across the RBCs. However, it should be noted that this short-term, immediate increase in microvascular oxygenation might be offset by the more deleterious increase in hemolysis arising from the increased cellular mechanical fragility and impaired deformability64.
Various research groups have investigated the potential of prolonging the duration of stored blood beyond the 42 days period through rejuvenating techniques65,66. In this study, RBCs were rejuvenated after 42 days of storage with pyruvate and inosine to restore the depleted intracellular 2,3-DPG so as to investigate the potential effect on the altered O2 release rate. Our results show that the restoration of 2,3-DPG to basal levels (Day 0) has no significant effect on the O2 release rate. This is supported by a previous clinical study67 which showed no improvement in tissue oxygenation in hypoxic patients with increased 2,3-DPG concentrations in RBCs. Apart from the assessment of stored blood, we envision our proposed D50 metric to be used clinically to complement the existing measure of P50 in disease diagnosis, monitoring, or even intervention1. This may be especially pertinent in diseases associated with altered hemoglobin (such as sickle cell anemia68 and beta thalassemia69) or impaired perfusion (such as diabetic microangiopathies70 and neurodegeneration71). It is of note that our proposed D50 metric, similar to the conventional P50, does not consider the effects of RBC perfusion on the overarching tissue oxygenation efficacy. Aging of RBCs has been known to be associated with the reduction of cellular deformability8. The impaired RBC deformability may lead to reduced perfusion at the tissue level and this is well documented in diseases such as malaria72, sickle cells anemia73, and hereditary RBC membrane disorders74. This reduction in perfusion, corresponding to longer capillary transit time, could complement the augmented D50 in enhancing tissue O2 delivery, possibly as a compensatory mechanism to maintain PO2 homeostasis.
In summary, this paper presents a MAP for determining the O2 release rate of RBCs (D50) at the single-cell level. In addition to P50 to assess the RBC’s capability of delivering O2, we use D50 to complement our assessment of the dynamic process of O2 delivery. Interestingly, based on our D50 results, biologically older RBCs exhibited a faster O2 release rate than younger RBCs. We further demonstrated that blood storage enhanced the RBC O2 release and confirmed that 2,3-DPG rejuvenation had no significant impact on restoring the O2 release rate. The single-cell level data acquired with the MAP provides additional information (D50) that fills the knowledge gap in the O2 delivery of RBCs.
Methods
Blood collection and sample preparation
This study was approved by the National University of Singapore Institutional Review Board (H-18-007) and all procedures were performed in accordance with the approved guidelines. Healthy male volunteers aged between 21 and 50 years old, without any known pathological conditions that could affect the properties of the collected blood, were recruited. The number of samples in each experiment was derived by equally distributing the total number of participants recruited. Informed consent was obtained from all recruited participants. No data from research participants were excluded.
All blood collection and storage processes were performed under sterile conditions. Whole blood (20 mL) was collected from the donors into citrate-phosphate-dextrose (CPD) (Terumo) in the ratio of 7:1. The blood was then centrifuged at 2500g for 10 min at 4 °C (Sigma 2-6, Goettingen, Germany) to remove the plasma and buffy coat. The top 2-mm layer of the packed RBCs was also removed to minimize the presence of leukocytes. To examine the effect of in vivo aging, packed RBCs were further density fractionated at 4600g for 30 min at 4 °C to obtain younger (top 20% fraction) and older (bottom 20%) RBCs75. To examine the effect of storage duration, the packed RBCs were resuspended in saline–adenine–glucose–mannitol (SAGM) additive solution (Terumo) to a hematocrit of 50–70% and stored under standard blood bank conditions (2–6 °C). RBC samples were aliquoted aseptically for measurements on Days 0, 1, 7, 14, 28, and 42. To restore intracellular 2,3-DPG, RBCs were incubated in a 10 mM solution of pyruvate and inosine for 1 h at 37 °C76.
Biochemical assay
MCHC and Hb were quantified using the direct cyanmethemoglobin77 with Drabkin’s reagent (D5941, Sigma), and the absorbance was recorded at 540 nm (Spark®, Tecan). Absolute 2,3-DPG was measured with a commercial kit (2,3-DPG, Roche). Normalized 2,3-DPG was obtained by normalizing the measured 2,3-DPG level by the corresponding MCHC from the same sample. Blood gases and pH were measured with a commercial blood gas analyzer (Epoc, Alere) and subsequently, the P50 values were determined78. Measurements for each sample were repeated successfully with similar results.
Microfluidic analytical platform
The MAP was fabricated from PDMS (Dow Corning, MI) by standard soft lithography and replica molding techniques. The MAP consists of an array of microwells for trapping single RBCs, which are separated from two flanking gas microchannels by a thin PDMS wall (Fig. 1a). The detailed information of the channel design has been provided in Supplementary Fig. 1. On-chip O2 concentrations in the microwells were determined by an O2 sensitive probe, tris(2,2′-bipyridyl)dichlororuthenium(II) hexahydrate (RTDP) (544981, Sigma), dissolved in 1× phosphate-buffered saline (PBS) at 1 mg/mL (pH 7.2). The fluorescence intensity (I) was monitored using our microscope system (IX71, Olympus) under epi-fluorescence (U-MWB2, Olympus). The collisional quenching of this probe by O2 is described by the first-order Stern–Volmer equation: \(I_0/I = 1 + K_q\left[ {{\rm{O}_{2}}} \right]\), where I0 is the intensity at 0% O2, Kq is the Stern–Volmer constant, and [O2] is the O2 concentration. The experimental Kq in our setup was determined as 2.2 × 10−3 µM−1, corresponding to previously reported values79,80.
RBC samples were washed with PBS twice before resuspending in RTDP at a hematocrit of 5%. The RBC suspension was then flowed into the MAP at 2 μL/min using a syringe pump (70-4504, Harvard Apparatus). After filling the microwells with RBCs, heavy mineral oil (330760, Sigma) was used to flush the residual RBCs and to isolate single RBCs in the microwell array. We introduced air flows in the two gas channels to initialize the O2 concentrations in the microwells to 100% saturation before starting the deoxygenation process. N2 gas was subsequently pumped through the gas channels under constant pressure (400 mbar) using a precision pressure controller (MCFS-EZ, Fluigent) to deoxygenate the microwells. Concurrently, the temporal variations of fluorescence intensity in the microwell were obtained from the fluorescent images (640 × 540 pixels, 4 × 4 binning) recorded with an sCMOS camera (Pco.edge, PCO AG, Kelheim, Germany) at 100 frame/s and a 40× objective (LCPlanFl, NA = 0.60, Olympus).
Statistics and reproducibility
Statistical analyses were performed using a commercially available software package (Graphpad Prism 4). Results are presented as means ± SD. The Mann–Whitney test (two-tailed) was used to assess the statistical significance between microwells with (n = 82 of biologically independent RBCs) and without RBCs (n = 127 of biologically independent RBCs). Unpaired Student’s t tests (two-tailed) were used to assess the statistical difference between younger and older RBCs for MCHC, absolute 2,3-DPG, normalized 2,3-DPG, and P50 (n = 5 of biologically independent samples). Mann–Whitney tests (two-tailed) was used to assess the statistical significance between younger (n = 93 of biologically independent RBCs) and older (n = 89 of biologically independent RBCs) RBCs for D50. One-way ANOVA was used to assess the statistical significance across the storage duration for MCHC, absolute 2,3-DPG, normalized 2,3-DPG, and P50 (n = 5 of biologically independent samples). Kruskal–Wallis test was used to assess the statistical significance across the storage duration for D50 (Day 0: n = 72, Day 1: n = 71, Day 7: n = 60, Day 14: n = 55, Day 28: n = 75 and Day 42: n = 65 of biologically independent RBCs). When appropriate, post hoc analyses were performed with Tukey’s honestly significant difference test. Paired Student’s t test (two-tailed) was used to analyze the data before and after RBC rejuvenation for MCHC, absolute 2,3-DPG, normalized 2,3-DPG, and P50 (n = 5 of biologically independent samples). Wilcoxon test was used to analyze the data before (n = 65 of biologically independent RBCs) and after (n = 93 of biologically independent RBCs) RBC rejuvenation for D50. Single-cell distribution profiles were created using the kernel probability distribution. To describe the distribution profiles, full width at half maximum (FWHM = 2.355×σ) and skewness \((\frac{n}{{(n - 1)(n - 2)}}{\sum} {\left( {\frac{{x_j - \mu }}{\sigma }} \right)^3} )\) were used, where µ and σ represent the mean and SD, respectively, n is the total number of observations and xj is the observation of j. The sample size for each experiment was reported in the figure caption. Data presentations were performed using MATLAB 2019a (MathWorks, USA).
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
All data are available in the main and supplementary files. Source data for the main figures can be found in Supplementary Data 1.
Code availability
The in-house codes used for the analyses in this study are available from the corresponding author upon reasonable request.
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Acknowledgements
This research was supported by the Singapore National Medical Research Council (NMRC)/Cooperative Basic Research Grant (CBRG)/0078/2014 and Institute for Health Innovation & Technology, NUS.
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K.Z.C. and Y.C.N. participated in the design of the MAP, design, and performance of all experiments and analyzed the data, alongside S.K. B.N. and J.K.S.T helped to design and perform the experiments. S.P. helped to perform the experiments and analyzed the data. S.L.T. and H.L.L. provided scientific inputs in the design and analysis of the experiment data. K.Z.C., Y.C.N., J.K.S.T., and S.K. prepared the paper. S.K. supervised all the work.
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Chng, K.Z., Ng, Y.C., Namgung, B. et al. Assessment of transient changes in oxygen diffusion of single red blood cells using a microfluidic analytical platform. Commun Biol 4, 271 (2021). https://doi.org/10.1038/s42003-021-01793-z
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DOI: https://doi.org/10.1038/s42003-021-01793-z
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