Plants respond to herbivory by perceiving herbivore danger signal(s) (HDS(s)), including “elicitors”, that are present in herbivores’ oral secretions (OS) and act to induce defense responses. However, little is known about HDS-specific molecules and intracellular signaling. Here we explored soybean receptor-like kinases (RLKs) as candidates that might mediate HDS-associated RLKs’ (HAKs’) actions in leaves in response to OS extracted from larvae of a generalist herbivore, Spodoptera litura. Fractionation of OS yielded Frα, which consisted of polysaccharides. The GmHAKs composed of their respective homomultimers scarcely interacted with Frα. Moreover, Arabidopsis HAK1 homomultimers interacted with cytoplasmic signaling molecule PBL27, resulting in herbivory resistance, in an ethylene-dependent manner. Altogether, our findings suggest that HAKs are herbivore-specific RLKs mediating HDS-transmitting, intracellular signaling through interaction with PBL27 and the subsequent ethylene signaling for plant defense responses in host plants.
To defend themselves against pest attack, plants are equipped with sophisticated sensory systems to perceive herbivore-derived danger signals (HDSs), including “elicitors”, that induce defense responses1. Herbivore oral secretions (OS) of several lepidopteran larvae and other arthropods contain an array of elicitors, including hydroxy fatty acid-amino acid conjugates (FACs; e.g., volicitin [N-(17-hydroxylinolenoyl)-l-glutamine])2,3,4. Although it has been shown that FAC-type elicitors induce depolarization of the plant cell plasma-membrane potential by activating voltage-dependent Ca2+ channels5, specific receptors for FACs have not been characterized6. Other distinct types of HDSs have been characterized from several herbivore species, such as β-glucosidase from cabbage white butterfly (Pieris brassicae)7, disulfooxy fatty acids (caeliferins) from the American bird grasshopper (Schistocerca americana)8, the peptide inceptins from fall armyworm (Spodoptera frugiperda)9, the peptide tetranins from spider mites (Tetranychus urticae)10, a porin-like protein from Egyptian cotton leafworm (Spodoptera littoralis)11, and a putative β-galactofuranose polysaccharide from S. littoralis12.
There is very little knowledge about the HDS recognition system and its downstream intracellular signaling in plants. One notable report showed that rice leucine-rich repeat (LRR) receptor-like kinase 1 (OsLRR-RLK1) is involved in defense responses of rice against striped stem borer (SSB)13. In other cases, rice and tobacco lectin receptor kinases (LecRKs) were shown to serve positively and negatively for defense responses of the host plants during damage by the herbivores Nilaparvata lugens and Manduca sexta, respectively14,15. Moreover, it has been shown that the Arabidopsis LRR-RLK, brassinosteroid insensitive1-associated kinase 1 (BAK1), is required for the defense response to aphid attack16. However, it remains to be clarified, for all of these membrane proteins, whether these proteins are specific to elicitors from the respective insects, and if so, what type of elicitor molecule(s) act as the HDS. More recently, a cowpea LRR-receptor-like protein (RLP) was shown to serve as inceptin receptor (INR), which is responsible for elicitor-induced responses and enhanced defense against armyworms (Spodoptera exigua) (https://www.biorxiv.org/content/10.1101/679803v1).
In contrast to the scant knowledge about HDSs, microbe-associated molecular patterns (MAMPs), including saccharide elicitors (e.g., chitin) and proteinaceous elicitors (e.g., flagellin and EF-Tu) have been well characterized, and found to be recognized by pattern recognition receptors (PRRs) located at the plasma membrane, including RLKs and RLPs17,18. For instance, chitin elicitor receptor kinase 1 (CERK1) is a member of an RLK family whose members are broadly involved in recognizing pathogenic saccharide elicitors (e.g., chitin, peptidoglycan, lipopolysaccharide, and β-glucan)19. In turn, BAK1, one of the LRR-RLKs that is bound to other LRR-RLK/RLP members, plays a crucial role in the recognition of proteinaceous elicitors (e.g., flagellin, EF-Tu, SCFE1, NLP, and endogenous Atpep)18. Following the perception of these MAMPs, CERK1-LYK5, a chitin receptor complex, transmits phosphorylation signals to receptor-like cytoplasmic kinase AtPBL27 and RLCK VII-4 members to activate the MAPK cascade for defense response in Arabidopsis20,21,22,23. Likewise, in addition to these aboveground systems, in rhizobial, symbiotic and immune signaling of soybeans, lipo-chitooligosaccharide Nod factors from rhizobial symbionts appear to be recognized via GmNFR1/GmNFR5 (lysine-motif (LysM)-RLK)24. Thus, PRRs (RLKs/RLPs) might be involved in a broad range of interactions between host plants and their partners or competitors, but to date, only a few HDS-specific RLKs have been characterized.
In the current study, we focused on soybean RLKs that act for recognition of elicitor(s) that are present in the OS of the generalist herbivore Spodoptera litura, and for the subsequent intracellular immune signaling. Here we show that soybean plasma membrane-localized HDS-associated receptor-like kinases (HAKs) play a significant role in eliciting defense responses of host plants mediated through an array of intracellular signaling activations.
Mining of the soybean HAKs
First, we focused on soybean genes that are homologs to Arabidopsis CERK1 (AtCERK1), which is involved in recognizing an array of pathogen-derived oligosaccharide elicitors, as described above19. Given that high molecular weight substances consisting of unknown oligosaccarides in OS of Spodoptera spp. function as putative elicitors in Arabidopsis host plants12, we focused on the RLK superfamily based on our hypothesis that some soybean HAKs (GmHAKs) are structurally similar to AtCERK1 (LysM-RLK), which may recognize various unknown elicitors. To identify and obtain the full-length open reading frame (ORF) sequence of putative soybean RLK cDNA clones based on in silico analyses of sequence similarity to AtCERK1, we then performed a BLASTX search using the ORF sequence of AtCERK1 using a Legume Base NBRP (https://www.legumebase.brc.miyazaki-u.ac.jp/) supported by the NBRP project (Lotus japonicus and Glycine max) office, which provides resources of full-length cDNA clones publically. Since the BLASTX search hit only a single clone of LysM-RLK (GMFL01-15-B07), we also expanded the scope of our interest in potential candidates to other structural variants of RLKs (LRR-RLKs and proline-rich extensin-like receptor kinases [PERKs]; Supplementary Table 1). As a result, we mined 19 soybean RLKs showing amino acid sequence similarity to AtCERK1 (~44% identities). Of these 19 putative soybean RLKs thus far mined as GmHAK candidates, the corresponding cDNAs of 15 genes were successfully amplified (Supplementary Fig. 1a), and we then generated the respective 15 transgenic Arabidopsis lines independently expressing RLK genes under the control of the constitutive cauliflower mosaic virus (CaMV) 35S promoter (35SP) (R1-R15) (Supplementary Table 1).
The defense ability of the transgenic lines was evaluated by monitoring the transcript levels of the jasmonate (JA)- and ethylene-inducible defensin gene PDF1.225 in leaves subjected to mechanical damage (MD) alone, or to MD with exogenous application of OS collected from S. litura larvae. Both MD alone and MD + OS enhanced PDF1.2 transcript levels in leaves of the R3 line in comparison to the levels in wild-type (WT) leaves (Fig. 1a). Note that the R3 protein has a GUB_WAK_bind domain, which is conserved in wall-associated kinases (WAKs), the specific receptors for oligogalacturonides released from the plant cell wall26, implying that R3-RLK has the same or similar function as Arabidopsis WAKs. In contrast, the R5 and R14 lines showed elevated PDF1.2 transcript levels only with MD + OS, relative to the levels in WT. These responses were confirmed using other genotypes of the identical foreign genes (R5-2 and R14-2), but not MD-inducible trypsin inhibitor (TI) expression (Supplementary Fig. 2), which further confirmed that R5 (H1)- and R14 (H2)-corresponding genes were responsible for defense responses in an OS-specific manner. We therefore redesignated the putative receptors expressed in transgenic lines R5 and R14 as GmHAK1 and GmHAK2, respectively, and the corresponding transgenic lines were renamed H1 and H2, and are so referred to hereafter.
GmHAKs are specific RLK candidates for Spodoptera OS
H1 and H2 leaves responded dramatically to not only S. litura OS but also to damage by S. litura by expressing PDF1.2, in comparison to those of the vector control (VC) plants (Figs. 1a, b). However, H1 and H2 leaves did not respond to MD plus other types of elicitors, including chitin oligosaccharides [(GlcNAc)7] and N-linolenoyl-l-Gln (18:3-Gln, a FAC elicitor from several lepidopteran larvae4), as indicated by their expression of PDF1.2 at similar levels to those in the vector control (VC) plants (Fig. 1c). Moreover, PDF1.2 transcript levels were not induced in Arabidopsis leaves by application of OS collected from the larvae of other lepidopteran species (Mythimna loreyi and Pieris rapae) (Supplementary Fig. 3a). Altogether, these findings imply that GmHAK1 and GmHAK2 are not likely to be responsive to the classical elicitors chitin and FAC (another elicitor that is present at very high levels in S. litura OS).
Structure and plasma-membrane localization of GmHAKs
Plant RLKs have a typical structure including a ligand-binding domain in the extracellular region, a single transmembrane region and a kinase domain in the intracellular region, and are located at the plasma membrane, where they function in perception of external stimuli such as elicitors18. Notable characteristics of GmHAK proteins were that they each possess a single predicted transmembrane region and a kinase domain (Fig. 1d), and that a leucine-rich repeat (LRR) and a proline-rich sequence (PR01217 [PRICHEXTENSN]) are present in the extracellular region of GmHAK1 and GmHAK2, respectively. Examining the subcellular localization of the GmHAKs by transient expression of GmHAK-GFP (green fluorescent protein) fusion protein in the epidermal cells of onion showed GFP signals of GmHAK1 and GmHAK2 along the plasma membrane (Fig. 1e).
Fractionation and characterization of OS components
Based on the experimental strategy employed in a previous study in which the OS from S. littoralis (closely related to S. litura) was fractionated12, we fractionated S. litura OS by size exclusion chromatography (SEC). Through the first round of SEC to separate components with molecular sizes of 100–1800 Da, three distinct groups consisting of major fractions (FrA, FrB, and FrC) were obtained (Fig. 2a). Of these, FrA was shown to induce elevated transcript levels of PDF1.2 in mechanically damaged VC leaves and to induce more drastically increased levels in H1 and H2 leaves (Fig. 2b). Through the second round of SEC to separate FrA into fractions with molecular sizes of 1500–20,000 Da, 14 major peaks were detected (Fig. 2a). Of the three major fractions (Frα [which contained peak 1], Frβ [which consisted of peaks 2–7], and Frγ [which consisted of peaks 8–14]), Frα, containing the highest molecular weight components, was able to increase the transcript levels of PDF1.2 in mechanically damaged leaves of the H1 and H2 lines to a higher level than those in VC leaves (Fig. 2b). Both FrA and Frα also induced the transcript levels of a pathogenesis-related (PR) gene in mechanically damaged soybean leaves (Supplementary Fig. 4).
Frα was not subjected to further fractionation by SEC because there was not an sufficient total quantity of material in it. We instead shifted to an alternative strategy to fractionate FrA, using normal-phase chromatography and reverse phase chromatography. Fractionation through reverse phase chromatography was not able to separate FrA into distinct components (Supplementary Fig. 5a). However, fractionation through normal-phase chromatography produced a major component peak, eluted with 40% water (FrII), which induced a higher level of the PDF1.2 transcript in mechanically damaged leaves of the H1 and H2 lines with respect to VC plants (Supplementary Figs. 5a, b). Moreover, when FrA was heated at 95 °C for 20 min or incubated with a 1% (w/w) protease cocktail at 37 °C overnight, its ability to induce an increase of the PDF1.2 transcript level was not impaired (Supplementary Fig. 3b), suggesting that the elicitor molecule(s) in the FrA subfractions Frα or FrII contained a single or multiple macromolecule(s) but did not mainly consist of protein(s) or peptide(s), in agreement with previous reports12.
1H-NMR analysis was performed to make a rough survey of the molecular species present in Frα and FrII (Supplementary Figs. 5c, d). We observed overlapping broad signals in the region of 3.2–4.5 ppm, which were typical of chemical shifts caused by sugar ring protons. These results were in accord with data from gas chromatography–mass spectrometry (GC-MS) analysis of acetylated methyl glycosides27, which showed fragmentation of the Frα components into hexoses (e.g., glucose, galactose and mannose) and a minor group of pentoses (e.g., arabinose, ribose and xylose) (Fig. 2c, Supplementary Fig. 6a). Furthermore, analysis of monosaccharide branching points28 of Frα showed the presence of pentofuranoside, hexopyranoside, 3-substituted hexopyranoside, and 3,6-di-substituted hexopyranoside. We therefore predicted that the FrA-fractionated Frα or FrII (predicted size >15 kDa) is a polysaccharide(s) containing deoxy sugar, or a complex macromolecule composed of sugar (Fig. 2d, Supplementary Fig. 6b).
Finally, the possibility that the artificial diet consumed by larvae might contribute to the metabolites and elicitor activity of OS was considered. To examine this possibility, we assessed the OS fractions collected from S. litura larvae that had been reared on Arabidopsis or soybean host plants. The OS fractionation resulted in distinct chromatographs with components ranging in size from 100 to 1800 Da (Supplementary Fig. 7a), but FrA (containing the highest molecular weight components) was most active in inducing the transcript level of PDF1.2 in mechanically damaged leaves of WT in both cases (Supplementary Fig. 7b). FrA induced stronger responses in the H1 and H2 lines than in the VC line (Supplementary Fig. 7c).
Homomultimers of GmHAKs do not bind Frα
To investigate the molecular interaction between HAKs and elicitors, we performed isothermal titration calorimetry (ITC) analysis using Frα and the extracellular domain of GmHAKs. No change of heat capacity was observed by the titration of Frα to cells containing GmHAKs or GFP control protein (Supplementary Fig. 8). Interestingly, GmHAK1 and GmHAK2 were each able to form homodimers, and these interactions were not dependent on the presence of Frα, according to the results of assays using the AlphaScreen system (Fig. 3a). These results were in accord with the observation of the interactions in vivo using a bimolecular fluorescence complementation (BiFC) assay. For that assay, proteins consisting of GmHAK1 or GmHAK2 fused to the N-terminal portion of Venus (GmHAK1-nVenus or GmHAK2-nVenus) and the C-terminal portion of Venus (GmHAK1-cVenus or GmHAK2-cVenus) were transiently expressed in the epidermal cells of onions (Fig. 3b). The fluorescent signals of these recombinant Venus proteins were detected along the plasma membrane upon expression of GmHAK1-nVenus with GmHAK1-cVenus, or GmHAK2-nVenus with GmHAK2-cVenus. However, the signals were not detected upon expression of GmHAK1-nVenus with GmHAK2-cVenus, or GmHAK2-nVenus with GmHAK1-cVenus (Fig. 3b). All of these findings indicate that homomultimers of GmHAKs may serve as Frα-non-interacting or low-affinity Frα-binding RLKs.
Function of GmHAKs in soybean defense responses
In order to assess the intrinsic function of GmHAKs in soybean defense responses, we obtained GmHAK knockdown transgenic soybean lines by employing the virus-induced gene silencing system using Apple latent spherical virus (ALSV) vectors29. In the soybean plants that had been inoculated with ALSV (lines RiH1 and RiH2), the levels of accumulation of GmHAK1 and GmHAK2 transcripts in leaves decreased to 6.6% and 27.9% in comparison to those in WT leaves, respectively (Fig. 4a). Instead, GmHAK1 homolog (GLYMA11G200300, 57% amino acid sequence identity to GmHAK1; Supplementary Fig. 1b) or GmHAK2 homolog (GLYMA07G004700, 57% amino acid sequence identity to GmHAK2; Supplementary Fig. 1b) did not show decreased transcript levels in leaves of RiH1 and RiH2, respectively, supporting the idea that knockdown of genes occurred specifically on the corresponding genes (Supplementary Fig. 9).
The knockdown plants exhibited more leaf damage by S. litura larvae compared with the damage exhibited by WT and VC plants (Fig. 4b). These results were in accord with the fact that the transcript levels of a PR gene in these knockdown plant leaves subjected to MD + Frα treatment were lower than those in WT and VC leaves (25.2% and 28.1%, respectively, of those in WT) (Fig. 4c). The transcript level of the gene for TI, a wound-responsive defense gene, was strongly induced by MD in all of the plant lines assessed, but MD + Frα treatment did not have significant effects on the transcript levels compared with MD alone (Fig. 4c).
Arabidopsis HAK and early signaling components in Arabidopsis
In order to deepen our understanding of plant intracellular signaling after OS perception, we next explored Arabidopsis genes homologous to GmHAKs. Based on BLASTP searches using the extracellular region sequence of both GmHAKs, we mined two AtHAK candidate genes showing amino acid sequence similarity to GmHAK1 (at1g06840 [57% identity] and at5g01950 [59% identity]). Of them, one T-DNA insertion mutant line (athak1, corresponding to at1g06840; Fig. 5a) showed a lower expression level of PDF1.2 in leaves, in comparison to that in WT leaves, after MD + FrA treatment (Fig. 5b, Supplementary Fig. 1b). This was confirmed by the similar response of another T-DNA insertion mutant line (athak1-2) to MD + FrA, as well as the response of athak1 to MD + Frα (Figs. 5b, 6a). On the other hand, when response abilities of the other T-DNA insertion mutant lines, corresponding to AtPBL27, PUB4, BIK1/PBL1, and XLG1/2/3 (well characterized as MAMP-associated kinases, E3 ubiqutin ligase or G proteins that act via a recognition complex and intracellular signaling), to MD + FrA treatment were assessed, a weaker response of PDF1.2 expression in AtPBL27 mutant (atpbl27) leaves was observed, in comparison to that in WT leaves (Fig. 5b). Again, as atpbl27 showed a weaker response to MD + Frα (Fig. 6a), we confirmed that AtHAK1 (at1g06840) and AtPBL27 (at5g18610) significantly contributed to FrA/Frα responses promoting Arabidopsis defense properties. These conclusions were in agreement with the observation that athak1 and atpbl27 exhibited enhanced development of S. litura larvae on the potted plants during 4 days (Fig. 6b). On the other hand, in response to either MD alone or MD + Frα, the endogenous levels of jasmonates (jasmonic acid [JA] and jasmonoyl-l-isoleucine [JA-Ile]) did not differ among WT and mutant leaves (Fig. 6c). In contrast, both athak1 and atpbl27 mutant leaves showed lower emission of ethylene in response to MD + Frα, compared with that from WT leaves (Fig. 6c). Our present findings accorded with the much lower expression level of PDF1.2 in leaves of ein2-1 (ethylene insensitive mutant30) in response to MD + Frα, compared with that in WT leaves (Fig. 6d). However, the PDF1.2 expression was also attenuated in the leaves of coi1-1 (jasmonate insensitive mutant31) (Fig. 6d), indicating altogether that Frα committed Arabidopsis to both biosynthesis and signaling of ethylene, whereas it committed this plant to jasmonate signaling but not jasmonate biosynthesis.
Note that neither the athak1 nor atpbl27 mutant showed any marked differences in plant growth, development or morphology (Supplementary Fig. 10a). Moreover, those mutants did not show impaired responses to jasmonates or ethylene, as root and hypocotyl development of athak1 or atpbl27 seedlings were observed to occur similarly to those in WT seedlings in the presence of methyl jasmonate (MeJA) and an ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), in contrast to the impairment of such development in coi1-1 and ein2-1 in the presence of MeJA and ACC, respectively (Supplementary Fig. 10b).
Next we assessed the interaction abilities of AtHAK1 and AtPBL27 molecules, using the AlphaScreen system (Fig. 7a). We found that (i) AtHAK1 formed a homodimer and (ii) similarly to AtCERK1, AtHAK1 was able to interact strongly with AtPBL27. Interactions of those molecules assessed using BiFC assays confirmed interactions between AtHAK1 molecules themselves or AtHAK1 and AtPBL27 along the plasma membrane of Nicotiana benthamiana leaves in BiFC assays using the Agrobacterium tumefaciens-mediated transient expression system (Fig. 7b). Moreover, immunoprecipitation assays also confirmed in vivo interactions between AtHAK1 molecules themselves or AtHAK1 and AtPBL27 in N. benthamiana leaves following their transient expression (Fig. 7c).
Finally, AlphaScreen system and BiFC assays preliminarily resulted in the observation of interactions between GmHAKs and AtPBL27 (Supplementary Fig. 11), implying that AtPBL27 might transmit the signal from the heterozygously expressed soybean HAKs for the defense response in the transgenic Arabidopsis leaves in response to S. litura OS (FrA or Frα) (see above).
HDS, including elicitor(s) present in herbivores’ OS, induce host plants’ defense responses mediated by early cellular responses (e.g., Ca2+ influx)32, hormone signaling (e.g., JA-signaling)33, and the activation of defense genes and biosynthesis of defense products34. HAKs were characterized here as RLKs that specifically respond to herbivore elicitors but not MD alone (Figs. 1a, 6a, Supplementary Fig. 2). HAKs are predicted to be Spodoptera spp-elicitor-specific RLKs (Fig. 1c). Of the HAKs found here, GmHAK1 and AtHAK1 were predicted to be LRR-RLKs (Fig. 1d). Other members of this group, BAK1, FLS2, and EFR, have been clearly shown to respond to pathogenesis peptide elicitors18, so it initially seemed possible that GmHAK1 and AtHAK1 might likewise perceive peptides present in or produced by degradation of proteins from S. litura OS. Although the 1H-NMR analysis of Frα or FrII, which showed minor signals in the aliphatic region (1–2.5 ppm) that were presumed to arise from protein/peptide molecules (Supplementary Figs. 5c, d), it seemed unlikely that GmHAK1 responded to peptides, because heat-denaturation or protease treatment of FrA scarcely suppressed the elicitor activity (Supplementary Fig. 3b). More importantly, given the assumption that GmHAK1 does not interact directly with the elicitor ligand (Supplementary Fig. 8), it is possible that GmHAK1 may be involved in amplification of molecular signals transmitted from the cognate FrA/Frα receptors.
On the other hand, GmHAK2 has a proline-rich sequence in its extracellular domain region (Fig. 1d), and thus this HAK is predicted to be a member of proline-rich extensin-like receptor kinases (PERKs). Fifteen PERK genes are predicted to be present in the Arabidopsis genome, and they may be involved in cell expansion, growth and floral development35. To our knowledge, GmHAK2 is annotated as a novel member of the PERK family that is involved in the plant defense system and that may also function as an HDS-transmitting and/or amplifying RLK.
It should be noted that we cannot exclude the possibilility that HAKs may act as partner membrane proteins (i.e., co-receptors) of receptor proteins that interact with Frα directly, as shown for rice OsCERK1 co-receptor, which cooperates with rice CEBiP and LYP4/6 to respond to chitin and peptidoglycan elicitors, as well as for Arabidopsis AtCERK1, which cooperates with LYM1/3, which are peptidoglycan receptors18,19. The extracellular domain of OsCERK1 has been shown to promote formation of OsCERK1 homodimer, which interacts with two CEBiP moleules, resulting in a sandwich-like complex, when chitin oligosaccharide elicitor binds to OsCERK119.
Given the fact that the HAKs found here may be composed of the respective homodimers (GmHAK1/GmHAK1, GmHAK2/GmHAK2, or AtHAK1/AtHAK1) (Figs. 3a, 7), we propose a possible model in which HAKs work as co-receptors (like OsCERK1) to transmit the phosphorylation signal to intracellular molecule(s). This accords with the structural characteristic of HAKs, which contain the arginine-aspartate (RD) type of kinase domain. The RD type kinases (e.g., BAK1 and CERK1) are thought to generate the initial phosphorylation signal by interacting with non-RD type receptor kinases (e.g., FLS2, EFR and LYK5) which lack kinase activity but can bind the ligand molecule and then activate the partner co-receptor(s)19,36. An alternative possibility is that the ITC system might not be appropriate for evaluating the HAK-Frα interactions or that Frα might be structurally modulated or degraded in planta prior to perception by HAKs (see below).
Regarding the partner protein for the intracellular signaling of HAKs, receptor-like cytoplasmic kinases (RLCKs) act as a major class of signal transmitting proteins after elicitor perception in Arabidopsis37. AtPBL27 (a member of the RLCKs) contributes to MAPK activation, defense gene activation, and callose deposition, but not to reactive oxygen species (ROS) generation, in chitin signaling in Arabidopsis19,21. A BLAST search for AtPBL27 showed about 80% identity of amino acid residues with a suite of soybean PBL27s (e.g., GLYMA15G096800 and GLYMA13G216100), suggesting that the FrA/Frα perception system consists of HAK1 and PBL27 in a variety of plant taxa, including Brassicaceae and Leguminosae.
In Arabidopsis, the involvement of jasmonate signaling, the primary signaling pathway for HDS-inducible defense response38, in OS signaling after herbivore attack should also be considered here. The HAK/PBL27 system is not likely to serve as an upstream signal for de novo jasmonate synthesis (Fig. 6c). Instead, the HAK/PBL27 system is more likely to be involved in the activation of ethylene synthesis and signaling, in agreement with our findings that an Arabidopsis mutant line that is ethylene insensitive (ein2-1) completely lacks expression of PDF1.2 in response MD + Frα (Fig. 6d). The same held true for JA-Ile receptor COI1 (coi1-1), indicating the involvement of the HAK/PBL27 system in jasmonate signaling but not jasmonate synthesis (Fig. 6d).
Polysaccharides are thought to be major components of Frα (or FrA and FrII) (Supplementary Figs. 2, 5). They are large molecules (>15 kDa; Fig. 2b), and classically characterized low-molecular-weight elicitors, including FAC-type elicitors6, peptide-type elicitors such as inceptin9, and fatty acid-type elicitors (e.g., caeliferins)8 do not appear likely to contribute to the elicitor activity of Frα. However, the possibility that Frα components are degraded by host plant enzymes such as polysaccharide-degrading enzymes, eventually resulting in the generation of low-molecular weight oligosaccharide elicitors that are recognized by PRRs in the host plants, has not been excluded. This possibility might accord with the observation that the ITC system showed no affinities of HAKs with the original molecular components of Frα (Supplementary Fig. 8). It is also likely that polysaccharide-degrading enzymes, including glucosidase and arabinofuranosidase localized at the cell wall/plasmadesmata or herbivory-associated cell component derivatives, may contribute to Frα degradation. Further comprehensive and unified studies on an array of trafficking, perception, amplifying, and transmitting systems for S. litura-derived HDS in the extracellular space as well as intracellular signaling for defense responses of plants, should be performed to deepen our understanding of the nature of the HAK-mediated signaling network.
Plants and insects
WT and T-DNA insertion mutant (athak1 [SALK_134409C], athak1-2 [GK_958D06], at5g01950 [SALK_009439], cerk1 [GABI_096F09]39, atpbl27 [GABI_001C07]21, pub4 [SALK_054373]40, bik1/pbl141, xlg1/2/342, bak1-4 [SALK_116202]43), ein2-1 and transgenic lines of Arabidopsis thaliana (Col-0) plants and soybean (Glycine max, cv. Enrei) plants were, respectively, grown in soil in climate-controlled rooms at 22 ± 1 °C with a photoperiod of 12 h (80 µE/m/s) and at 24 ± 1 °C with a photoperiod of 16 h (80 µE/m/s). The individual plants were grown in single plastic pots. The potted, 4-week-old Arabidopsis plants and 2-week-old soybean plants were used for subsequent analyses. The coi1-1 seeds were germinated on 1/2 Murashige and Skoog medium supplemented with 2% sucrose, 0.8% agar, and 50 µM MeJA (Wako Pure Chemical Industrials, Ltd., Osaka, Japan) to screen for the individuals showing normal root growth for 2 weeks. The screened plants were transferred and grown in plastic pots for an additional 3 weeks for the assay44 shown in Fig. 6d.
Eggs of S. litura (Fabricius) were obtained from Sumika Technoservice Co. Ltd. (Takarazuka, Japan). They were incubated in a climate-controlled room at 24 ± 1 °C with a photoperiod of 16 h. The hatched larvae were reared on artificial diet (Insecta LFS, Nihon Nosan Kogyo Ltd., Tokyo, Japan) in a plastic container (0.9 L) with a mesh-covered lid. Feces in the plastic case were removed and a piece of artificial diet was added three times a week. When larvae reached the final instar, they were transferred to a larger container (1.6 L) with a mesh-covered lid. Finally, about 30 pupae of S. litura were transferred to a lidded plastic cylinder (ø 12 cm × 33 cm) and reared until their adult stage. Meanwhile, their oviposition was allowed on a piece of paper that completely covered the bottom of the cylinder. The eggs and larvae were used for assays or for continuous rearing on artificial diet (see above), except for the larvae reared after hatching for 1 week on fresh Arabidopsis or soybean plants in the laboratory at 24 ± 1 °C and used for analyses shown in Supplementary Fig. 7.
Adults of P. rapae were collected in a field in Katsushika-ku, Tokyo. The larvae were hatched and reared on Brassica rapa var. perviridis.
Primers used for all the polymerase chain reactions (PCRs) in this study are listed in Supplementary Table 2.
Transgenic Arabidopsis plants of soybean RLKs
The full-length ORFs of soybean PRR homologs were obtained from Legume Base NBRP (https://www.legumebase.brc.miyazaki-u.ac.jp/). The ORF of each clone was amplified using KOD Plus Neo (Toyobo, Osaka, Japan) and a pair of specific primers (Supplementary Table 2) and inserted into binary vector pMDC32 (2x 35SP::Gateway (GW) region::nopaline synthase terminator [NOST]) using the Gateway cloning system (Thermo Fisher Scientific, Waltham, MA, USA). The resulting vector pMDC32-GmHAK was transformed into A. tumefaciens strain EHA105 by electroporation. Col-0 WT Arabidopsis plants that had been grown for 6–7 weeks were transformed via the floral-dip transformation method and selected45. T3 homozygous plant lines were used for further analyses.
SEC of OS
Larvae were reared on artificial diet or fresh Arabidopsis plants (about 4 weeks old) or soybean plants (see above). OS was collected from third or fourth instar larvae of S. litura using a glass capillary tube (Hirschmann Laborgeräte GmbH & Co. KG, Eberstadt, Germany). M. loreyi OS was previously obtained in Okayama University46. The collected OS was stored at −20 °C until use.
To carry out SEC, crude OS was lyophilized using an FDU-1200 freeze dryer (EYELA, Tokyo, Japan). About 150 mg of lyophilized OS was dissolved with 3 mL of 50 mM ammonium bicarbonate (AMBIC) buffer (pH 8.0). For the first-round fractionation, OS was passed through a column (1.5 cm × 98.5 cm) packed with Bio-Gel P-2 resin (Bio-Rad, Hercules, USA), and 1.5 mL fractions were collected (yielding FrA to C). All of the fractions were lyophilized and then dissolved with 150 µL AMBIC buffer for the measurement of refractive index (10-fold concentrated) or with 750 µL of 10 mM MES buffer (pH 6.0) for assays (2-fold concentrated). FrA (about 70 mg) was purified through a Bio-Gel P-10 resin (Bio-Rad) column (2.0 cm × 45 cm). Fractions (1.5 mL) were collected during the course of elution (yielding Frα to γ). Again, all the fractions were lyophilized and then dissolved with 150 µl of AMBIC buffer for the measurement of refractive index (10-fold concentrated) or with 750 µl of 10 mM MES buffer (pH 6.0) for assays (2-fold concentrated). The temperature-corrected refractive index of fractions was monitored using a Brix/RI-Chek Refractometer (Reichert, NY, USA).
Characterization of FrA/Frα
For protease treatment of FrA, 3 mg of the lyophilized fraction were dissolved in 50 mM AMBIC buffer (pH 8.0) and digested with 1% (w/w) protease XIV (Sigma-Aldrich, St. Louis, MO, USA) for 24 h at 37 °C to degrade proteins, and boiled at 80 °C for 20 min to deactivate proteases.
The purification trials of FrA were performed using the CombiFlash Rf purification system (Teledyne ISCO, Lincoln, NE, USA). Pre-packed cartridges, RediSep Rf Gold C18Aq (Teledyne ISCO) and RediSep Rf Gold Diol (Teledyne ISCO) were used, respectively, for reversed- and normal-phase chromatography. The reversed-phase column (5.5 g media) was previously conditioned with phase A (pure water) and eluted with a 5.7-min linear gradient from 0 to 100% phase B (acetonitrile). For the normal-phase chromatography, the column (5.5 g media) was previously conditioned with phase A (acetonitrile) and eluted with a 5.7-min linear gradient from 0 to 100% phase B (pure water).
For proton nuclear magnetic resonance (1H-NMR) analysis, a lyophilized Frα or FrII sample (5 mg) was dissolved in 0.7 mL of D2O, and the spectrum was recorded using a Bruker Avance DRX-600 spectrometer (Bruker, Billerica, MA, USA) at 298 K.
Sugar components and the linkage pattern of monosaccharides in Frα were determined by the acetylated methyl glycosides method27 and the partially methylated alditol acetates method28, respectively. Monosaccharide derivatives were detected using the GCMS-QP2010 Plus GC-MS system (Shimadzu, Kyoto, Japan) with an HP-5MS capillary column (Agilent Technologies, Santa Clara, CA, USA). The carrier gas was He, with a 10 mL/min flow, injection volume 1 µL in acetone, injection port 220 °C with 1:2.5 split ratio. GC-oven temperature was programmed to rise from 40 °C (2 min hold) to 280 °C (10 min hold) at 10 °C/min. Electron ionization spectrometer range was 45–500 m/z.
OS and herbivore treatment
All of the crude OS and the fractionated OS samples were diluted 3- and 5-fold with 10 mM MES buffer (pH 6.0), respectively. Chitin oligosaccharide [(GlcNAc)7] was diluted to 10 µM with 10 mM MES buffer (pH 6.0). 18:3-Gln was diluted to 100 µM in 10 mM MES buffer (pH 6.0) with 0.01% Tween20. Mechanical damage (MD) was performed with stainless steel needles on 4 leaves of an individual Arabidopsis plant and one of the secondary leaves of individual soybean plants. Approximately 30 MD spots were made per leaf. The crude OS, the fractionated OS, chitin oligosaccharide or 18:3-Gln solution was immediately applied onto an MD spot (~1 µL per spot). Treatment of MD leaves with MES buffer served as a control.
RNA extraction, cDNA synthesis, and quantitative PCR
Approximately 100 mg of leaf tissues were homogenized in liquid nitrogen, and total RNA was isolated and purified using Sepasol®-RNA I Super G (Nacalai Tesque, Kyoto, Japan) following the manufacturer’s protocol. Single-stranded cDNA was synthesized using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo), and 0.5 µg of the total RNA was incubated, first at 37 °C for 5 min for the DNase reaction and then at 37 °C for 15 min for the RT reaction. Real-time PCR was performed using a CFX Connect real-time PCR detection system (Bio-Rad) with THUNDERBIRD SYBR qPCR Mix (Toyobo) and gene-specific primers (Supplementary Table 2). The following protocol was used: an initial polymerase activation of 60 s at 95 °C, followed by 45 cycles of 15 s at 95 °C and then 30 s at 60 °C. Then, a melting curve analysis preset by the instrument was performed10. Relative transcript abundances were determined after normalization of raw signals with the abundance of the housekeeping transcript of the Arabidopsis ACT8 gene (at1g49240) or soybean ACT gene (GQ339774.1). We did not use samples or data when sufficient amounts or quality of RNA (> 83 ng/µL) were not obtained from leaves or when abnormal quantification cycle (Cq) values for the actin gene were obtained.
Subcellular localization of recombinant GmHAK proteins
The full-length ORFs of GmHAK1 and GmHAK2 (without stop codon) were cloned in Gateway (GW) vector pGWB451 or pGWB405 (35SP::GW::GFP::NOST)47, resulting in the construction of vectors pGWB405-GmHAK1-GFP and pGWB451-GmHAK2-GFP. These vectors, plus pGWB452 (35SP::GFP::GW), which served as a control, were transiently transformed into onion epidermal cells. The cells were bombarded with 15 µg of vector coated onto 3 mg of tungsten particles (Rare Metallic Co., LTD, Tokyo, Japan) using the PDS-1000/He particle delivery system (Bio-Rad). Onion cells were incubated in the dark at 28 °C for 24 h after bombardment, and GFP fluorescence was observed under a fluorescence microscope BZ-X700 (Keyence Co., Osaka, Japan). For plasmolysis, the sample was treated with 0.6M mannitol for 15 min before observation.
Preparation of recombinant GmHAK proteins and ITC analysis
The extracellular domain of GmHAK1 (amino acid residues 24–556) and the full-length ORF of GFP were subcloned into pET23a. Similarly, the extracellular domain of GmHAK2 (amino acid residues 1–159) was subcloned into pDEST17. The recombinant vectors were transformed into Escherichia coli BL21-CodonPlus(DE3) to prepare the recombinant proteins. The proteins were extracted and purified using Ni Sepharose High Perfomance (GE Healthcare, Buckinghamshire, UK) following the manufacturer’s protocol. The protein was further purified with a gel filtration chromatography column, HiLoad 26/60 Superdex 200 (GE Healthcare), equilibrated with buffer (20 mM HEPES buffer (pH 8.0), 300 mM NaCl, 10% glycerol). Fractionated protein was concentrated with Amicon Ultra-15 (Merck Millipore Ltd, Darmstadt, Germany). Each of the boiled proteins in the sample buffer was subjected to 14% sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE).
Molecular interaction analysis between recombinant GmHAK proteins and Frα was performed using the MicroCal iTC200 system (Malvern Panalytical, Malvern, UK). Frα (2.5 mg/mL) was injected through the computer-controlled microsyringe (40 μL) at an interval of 2 min into the sample cells (cell volume = 200 μL) filled with recombinant GmHAK1 (25 µM) or GmHAK2 (50 µM) or GFP reference protein (50 µM) while stirring at 1000 rpm. The entire experiment was conducted at 30 °C. All ITC data were controlled by subtraction of the reference experimental data that were obtained for the titration of Frα against protein-free buffer. The data obtained were analyzed and the figure was prepared using the Origin 7.0 software package provided by Malvern Panalytical.
Cell-free protein synthesis, immunoblotting, and AlphaScreen system
The full-length ORFs of GmHAK1, GmHAK2, AtHAK1, CERK1, and AtPBL27 were inserted into the GW vector pEU-GW-AGIA, pEU-GW-bls (bls; biotin ligation site), or pEU-GW-FLAG for cell-free protein synthesis, resulting in the construction of vectors pEU-GmHAK1-AGIA, pEU-GmHAK2-AGIA, pEU-GmHAK1-bls, pEU-GmHAK2-bls, pEU-AtHAK1-bls, pEU-AtPBL27-bls, pEU-CERK1-FLAG, pEU-AtHAK1-FLAG, pEU-GmHAK1-FLAG, and pEU-GmHAK2-FLAG. Cell-free protein synthesis, AlphaScreen-based protein–protein interaction assays, and immunoblotting were carried out according to the methods described previously48. Frα was added at 0, 0.4, 4, or 40 µg/mL. For quality checking of the proteins used, total proteins were subjected to 10% SDS-PAGE and immunoblotted with AGIA HRP-linked antibody48, anti-Biotin HRP-linked antibody (Cell Signaling Technology, Beverly, MA, USA), or monoclonal anti-FLAG M2-peroxidase antibody produced in mouse clone M2 (Sigma-Aldrich).
The full-length ORFs of GmHAK1, GmHAK2, AtHAK1, and AtPBL27 (without stop codon) were inserted into the GW vector pDEST-GW-nVenus, along with the N-terminal fragment of Venus, and pDEST-GW-cVenus, along with the C-terminal fragment of Venus, respectively, resulting in the construction of vectors 35SP-GmHAK1-nVenus, 35SP-GmHAK2-nVenus, 35SP-GmHAK1-cVenus, and 35SP-GmHAK2-cVenus. Each pairwise combination of vectors (15 µg of each vector) was transiently co-transformed into onion epidermal cells using the PDS-1000/He particle delivery system (see above).
For the A. tumefaciens-mediated transient expression system using N. benthamiana grown for 3 weeks, a pair of A. tumefaciens EHA105 carrying the indicated vectors 35SP-AtHAK1-nVenus, 35SP-GmHAK1-nVenus, or 35SP-GmHAK2-nVenus, together with 35SP-AtHAK1-cVenus or 35SP-AtPBL27-cVenus, were pressure-infiltrated into the leaves of N. benthamiana as reported40.
Virus-induced gene silencing
cDNA fragments of GmHAK1 (162 bp) and GmHAK2 (180 bp), were amplified by PCR using primer pairs containing XhoI and BamHI sites (Supplementary Table 2) and ligated to pEALSR2L5R529, resulting in the construction of pEALSR2L5R5-GmHAK1 and pEALSR2L5R5-GmHAK2, respectively. Viral inoculation of soybean plants was carried out according to the method described previously29. Briefly, Chenopodium quinoa was inoculated with 20 µg of vector mixture (pEALSR1, pEALSR2L5R5-GmHAK1, or pEALSR2L5R5-GmHAK2) by mechanical wounding using carborundum. RNA was extracted from virus-infected C. quinoa leaves, and 400 µg of total RNA was coated onto 16 mg of tungsten particles. Soybean cotyledons were bombarded 10 times at 1100 psi using a PDS-1000/He particle delivery system (see above). The plants were grown in soil for 3 weeks and used for analyses.
A third-instar larva of S. litura, starved overnight, was released onto one of the secondary leaves of a potted soybean plant. The leaf was covered with a mesh bag and kept for 24 h. The leaves were then scanned, and the total leaf area and the consumed leaf area were determined using ImageJ. Replicate analyses were conducted with 9–11 independent samples.
We also performed assays to assess the growth of S. litura larvae on Arabidopsis plants. Third-instar larvae were initially weighed (1.8–2.0 mg), and each larva was released onto a potted plant. The net body weight that S. litura larvae gained at 22 ± 1 °C (14 h photoperiod at 80 µE/m/s) during 2 and 4 days after they had been released onto the plants was determined. When a larva was dead or lost during assays, we excluded that sample, and final replicate analyses were conducted with 12 independent samples44.
Phytohormone levels of leaves subjected to MD alone or MD + Frα treatment or of untreated leaves were determined. The jasmonate levels of leaves (60–100 mg fresh weight) were determined according to the method described previously49, with slight modifications.
Ethylene was measured in the headspace (45 mL) of 15 cut mature leaflets prepared from 3 plants. Ethylene was allowed to accumulate for 24 h at normal photoperiod, after which 1 mL of headspace air was removed by syringe with needle inserted through a silicon plug. Air samples were introduced to gas chromatograph GC-2014 (Shimadzu) equipped with Shincarbon ST stainless steel column (length 2 m; ID 3.0 mm; SHINWA Chemical Industries, Ltd., Kyoto, Japan) via manual injection port kept at 200 °C. Detector was flame ionization (FID) held at 210 °C. Column was kept at constant temperature 200 °C and helium flow 50 mL/min. Peak area was compared with ethylene concentration obtained from external 0–1 ppm calibration curve of ethylene standard (GL Sciences Inc., Tokyo, Japan).
The full-length ORFs of AtHAK1 and AtPBL27 (without stop codon) were inserted into the GW vector pGWB11 (35SP::GW::FLAG) or pGWB14 (35SP::GW::3xHA). As in BiFC assays, A. tumefacience EHA105 carrying each vector was pressure-infiltrated into the leaves of N. benthamiana40. After 2 days, total proteins were extracted with extraction buffer (50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 10% glycerol, 5 mM DTT, 2 mM EDTA, 1 mM NaF, 1 mM Na2MoO4–2H2O, 0.5% polyvinylpyrolidone, 1% NP-40, and Complete Protease Inhibitor Cocktail tablets (Roche Applied Science, Indianapolis, IN, USA)). Extracted proteins were incubated overnight with anti-DYKDDDDK-tag antibody magnetic beads (#017-25151, Wako Pure Chemical Industrials, Ltd.) at 4 °C, and the beads were washed four times with TBS containing 0.5% NP-40. Immunoprecipitates were eluted with Laemmli SDS sample buffer and used for 10% SDS-PAGE. Anti-FLAG antibody (#F3165, Sigma-Aldrich) and anti-HA antibody (#11867423001, Roche Applied Science) were sued as the primary antibodies. Horseradish peroxidase-linked anti-mouse antibody (#7076, Cell Signaling Technology) or anti-rat antibody (#7077, Cell Signaling Technology) was used as the secondary antibody. The membranes were soaked with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore) and the signals were detected with an ImageQuant LAS-4000 imaging system (GE Healthcare).
Statistics and reproducibility
We performed one-way ANOVA with Holm’s sequential Bonferroni post hoc test and post hoc Tukey’s HSD using the program (http://astatsa.com/OneWay_Anova_with_TukeyHSD/) for comparing multiple samples. The sample sizes and number of replicates for all the sets of assays and analyses are indicated in the legends of the corresponding Figures.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Mithöfer, A. & Boland, W. Recognition of herbivory-associated molecular patterns. Plant Physiol. 146, 825–831 (2008).
Maffei, M. E., Arimura, G. & Mithöfer, A. Natural elicitors, effectors and modulators of plant responses. Nat. Prod. Rep. 29, 1269–1368 (2012).
Alborn, H. T. et al. An elicitor of plant volatiles from beet armyworm oral secretion. Science 276, 945–949 (1997).
Spiteller, D. & Boland, W. N-(17-Acyloxy-acyl)-glutamines: novel surfactants from oral secretions of lepidopteran larvae. J. Org. Chem. 68, 8743–8749 (2003).
Maffei, M. E., Mithöfer, A. & Boland, W. Before gene expression: early events in plant-insect interaction. Trends Plant Sci. 12, 310–316 (2007).
Bonaventure, G., VanDoorn, A. & Baldwin, I. T. Herbivore-associated elicitors: FAC signaling and metabolism. Trends Plant Sci. 16, 294–299 (2011).
Mattiacci, L., Dicke, M. & Posthumus, M. A. β-Glucosidase: an elicitor of herbivore-induced plant odor that attracts host-searching parasitic wasps. Proc. Natl Acad. Sci. USA 92, 2036–2040 (1995).
Alborn, H. T. et al. Disulfooxy fatty acids from the American bird grasshopper Schistocerca americana, elicitors of plant volatiles. Proc. Natl Acad. Sci. USA 104, 12976–12981 (2007).
Schmelz, E. A. et al. Fragments of ATP synthase mediate plant perception of insect attack. Proc. Natl Acad. Sci. USA 103, 8894–8899 (2006).
Iida, J. et al. Tetranins: new putative spider mite elicitors of host plant defense. N. Phytol. 224, 875–885 (2019).
Guo, H. et al. A porin-like protein from oral secretions of Spodoptera littoralis larvae induces defense-related early events in plant leaves. Insect Biochem. Mol. Biol. 43, 849–858 (2013).
Bricchi, I. et al. Separation of early and late responses to herbivory in Arabidopsis by changing plasmodesmal function. Plant J. 73, 14–25 (2012).
Hu, L. et al. OsLRR-RLK1, an early responsive leucine-rich repeat receptor-like kinase, initiates rice defense responses against a chewing herbivore. N. Phytol. 219, 1097–1111 (2018).
Gilardoni, P. A., Hettenhausen, C., Baldwin, I. T. & Bonaventure, G. Nicotiana attenuata LECTIN RECEPTOR KINASE1 suppresses the insect-mediated inhibition of induced defense responses during Manduca sexta herbivory. Plant Cell 23, 3512–3532 (2011).
Liu, Y. et al. A gene cluster encoding lectin receptor kinases confers broad-spectrum and durable insect resistance in rice. Nat. Biotechnol. 33, 301–305 (2015).
Prince, D. C., Drurey, C., Zipfel, C. & Hogenhout, S. A. The leucine-rich repeat receptor-like kinase BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 and the cytochrome P450 PHYTOALEXIN DEFICIENT3 contribute to innate immunity to aphids in Arabidopsis. Plant Physiol. 164, 2207–2219 (2014).
Boller, T. & Felix, G. A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annu. Rev. Plant Biol. 60, 379–406 (2009).
Couto, D. & Zipfel, C. Regulation of pattern recognition receptor signalling in plants. Nat. Rev. Immunol. 16, 537–552 (2016).
Desaki, Y., Kohari, M., Shibuya, N. & Kaku, H. MAMP-triggered plant immunity mediated by the LysM-receptor kinase CERK1. J. Gen. Plant Pathol. 85, 1–11 (2019).
Yamada, K. et al. The Arabidopsis CERK1-associated kinase PBL27 connects chitin perception to MAPK activation. EMBO J. 35, 2468–2483 (2016).
Shinya, T. et al. Selective regulation of the chitin-induced defense response by the Arabidopsis receptor-like cytoplasmic kinase PBL27. Plant J. 79, 56–66 (2014).
Bi, G. et al. Receptor-like cytoplasmic kinases directly link diverse pattern recognition receptors to the activation of mitogen-activated protein kinase cascades in Arabidopsis. Plant Cell 30, 1543–1561 (2018).
Rao, S. et al. Roles of receptor-like cytoplasmic kinase VII members in pattern-triggered immune signaling. Plant Physiol. 177, 1679–1690 (2018).
Tóth, K. & Stacey, G. Does plant immunity play a critical role during initiation of the legume-rhizobium symbiosis? Front. Plant Sci. 6, 401 (2015).
Brown, R. L., Kazan, K., McGrath, K. C., Maclean, D. J. & Manners, J. M. A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis. Plant Physiol. 132, 1020–1032 (2003).
Brutus, A., Sicilia, F., Macone, A., Cervone, F. & De Lorenzo, G. A domain swap approach reveals a role of the plant wall-associated kinase 1 (WAK1) as a receptor of oligogalacturonides. Proc. Natl Acad. Sci. USA 107, 9452–9457 (2010).
De Castro, C., Parrilli, M., Holst, O. & Molinaro, A. in Methods in Enzymology, Vol 480 (ed. Fukuda, M.) 89–115 (Elsevier Academic Press Inc, 2010).
Björndal, B., Lindberg, B. & Svensson, S. Mass spectrometry of partially methylated alditol acetates. Carbohyd. Res. 5, 433–440 (1967).
Yamagishi, N. & Yoshikawa, N. Virus-induced gene silencing in soybean seeds and the emergence stage of soybean plants with Apple latent spherical virus vectors. Plant Mol. Biol. 71, 15–24 (2009).
Guzman, P. & Ecker, J. R. Exploiting the triple response of Arabidopsis to identify ethylene-related mutants. Plant Cell 2, 513–523 (1990).
Xie, D. X., Feys, B. F., James, S., Nieto-Rostro, M. & Turner, J. G. COI1: an Arabidopsis gene required for jasmonate-regulated defense and fertility. Science 280, 1091–1094 (1998).
Zebelo, S. A. & Maffei, M. E. Role of early signalling events in plant-insect interactions. J. Exp. Bot. 66, 435–448 (2015).
Okada, K., Abe, H. & Arimura, G. Jasmonates induce both defense responses and communication in monocotyledonous and dicotyledonous plants. Plant Cell Physiol. 56, 16–27 (2015).
Shinya, T. et al. Integration of danger peptide signals with herbivore-associated molecular pattern signaling amplifies anti-herbivore defense responses in rice. Plant J. 94, 626–637 (2018).
Humphrey, T. V. et al. PERK-KIPK-KCBP signalling negatively regulates root growth in Arabidopsis thaliana. J. Exp. Bot. 66, 71–83 (2015).
Macho, A. P. & Zipfel, C. Plant PRRs and the activation of innate immune signaling. Mol. Cell 54, 263–272 (2014).
Liang, X. & Zhou, J. M. Receptor-like cytoplasmic kinases: central players in plant receptor kinase-mediated signaling. Annu. Rev. Plant Biol. 69, 267–299 (2018).
Schmelz, E. A., Engelberth, J., Alborn, H. T., Tumlinson, J. H. 3rd & Teal, P. E. A. Phytohormone-based activity mapping of insect herbivore-produced elicitors. Proc. Natl Acad. Sci. USA 106, 653–657 (2009).
Miya, A. et al. CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis. Proc. Natl Acad. Sci. USA 104, 19613–19618 (2007).
Desaki, Y. et al. PUB4, a CERK1-interacting ubiquitin ligase, positively regulates MAMP-triggered immunity in Arabidopsis. Plant Cell Physiol. 60, 2573–2583 (2019).
Zhang, J. et al. Receptor-like cytoplasmic kinases integrate signaling from multiple plant immune receptors and are targeted by a Pseudomonas syringae effector. Cell Host Microbe 7, 290–301 (2010).
Ding, L., Pandey, S. & Assmann, S. M. Arabidopsis extra-large G proteins (XLGs) regulate root morphogenesis. Plant J. 53, 248–263 (2008).
Chinchilla, D. et al. A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. Nature 448, 497–500 (2007).
Miyamoto, T. et al. Tyrosine kinase-dependent defense responses against herbivory in Arabidopsis. Front. Plant Sci. 10, 776 (2019).
Ali, M. R. M. et al. The ring-type E3 ubiquitin ligase JUL1 targets the VQ-motif protein JAV1 to coordinate jasmonate signaling. Plant Physiol. 179, 1273–1284 (2019).
Shinya, T. et al. Modulation of plant defense responses to herbivores by simultaneous recognition of different herbivore-associated elicitors in rice. Sci. Rep. 6, 32537 (2016).
Nakagawa, T. et al. Improved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants. Biosci. Biotechnol. Biochem. 71, 2095–2100 (2007).
Yano, T. et al. AGIA tag system based on a high affinity rabbit monoclonal antibody against human dopamine receptor D1 for protein analysis. PLoS ONE 11, e0156716 (2016).
Tzin, V. et al. Rapid defense responses in maize leaves induced by Spodoptera exigua caterpillar feeding. J. Exp. Bot. 68, 4709–4723 (2017).
We would like to thank Dr. Hanae Kaku (Meiji University) for kindly providing cerk1 seeds and chitin oligosaccharides [(GlcNAc)7]. 18:3-Gln, M. loreyi OS, atpbl27 seeds were provided by Dr. Tomonori Shinya (Okayama University). The seeds of bik1/pbl1 were provided by Dr. Jian-Min Zhou (Chinese Academy of Sciences). The seeds of xlg1/2/3 were provided by Dr. Shinichiro Sawa (Kumamoto University). The vectors pGWB405, pGWB451, and pGWB452 were provided by Dr. Tsuyoshi Nakagawa (Shimane University). The virus vectors (pEALSR1 and pEALSR2L5R5) and BiFC vectors were provided by Dr. Nobuyuki Yoshikawa (Iwate University) and Dr. Jörg Kudla (University of Münster), respectively. Phytohormone analyses were assisted by Ms. Yuko Hojo (Okayama University). This work was financially supported in part by MEXT Grants-in-Aid for Scientific Research on Innovative Areas to G.A. (18H04786 and 20H04786), JSPS KAKENHI to T.U. (20K15878), Y.D. (20K06058), and G.A. (20H02951), and by the Joint Usage/Research Center, Institute of Plant Science and Resources, Okayama University.
The authors declare no competing interests.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Uemura, T., Hachisu, M., Desaki, Y. et al. Soy and Arabidopsis receptor-like kinases respond to polysaccharide signals from Spodoptera species and mediate herbivore resistance. Commun Biol 3, 224 (2020). https://doi.org/10.1038/s42003-020-0959-4
BMC Plant Biology (2021)
Trends in Plant Science (2020)
Proceedings of the National Academy of Sciences (2020)
Frontiers in Plant Science (2020)