Genetic damage in the germline induced by P-element mobilization causes a syndrome known as P-M hybrid dysgenesis (HD), which manifests as elevated mutation frequency and loss of germline cells. In this study, we found that Myc plays an important role in eliminating germline cells in the context of HD. P-element mobilization resulted in downregulation of Myc expression in the germline. Myc knockdown caused germline elimination; conversely, Myc overexpression rescued the germline loss caused by P-element mobilization. Moreover, restoration of fertility by Myc resulted in the production of gametes with elevated mutation frequency and reduced ability to undergo development. Our results demonstrate that Myc downregulation mediates elimination of germline cells with accumulated genetic damage, and that failure to remove these cells results in increased production of aberrant gametes. Therefore, we propose that elimination of germline cells mediated by Myc downregulation is a quality control mechanism that maintains the genomic integrity of the germline.
The germline is the only cell lineage that transmits genetic material to the next generation. Genetic damage in the germline causes developmental defects and genetic diseases in progeny. Therefore, suppression of genetic damage and elimination of aberrant germline cells are thought to play a critical role in maintaining the germline integrity of a species1,2,3,4. Mobilization of transposable elements, a leading cause of genetic damage in the germline, is suppressed by the Piwi-interacting RNA pathway at the transcriptional and posttranscriptional levels5,6,7,8,9,10,11,12. However, little evidence exists to support the germline elimination caused by genetic damage. To address this issue, we induced the elimination of germline cells by mobilizing P-elements and identified genes rescuing the elimination. We predicted that failure to remove germline cells harboring genetic damage by altering the functions of these genes would increase production of aberrant gametes.
In Drosophila, P-M hybrid dysgenesis (HD) is a sterility syndrome attributed to P-element mobilization13,14. When females lacking P-elements (referred to as the M strain) are crossed to males carrying P-elements (referred to as the P strain), mobilization of P-elements occurs in the germline of their progeny, which in turn exhibit a germline-loss phenotype13,14,15,16. This germline-loss phenotype is thought to be caused by DNA damage associated with P-element mobilization17. In ovarian germline cells, DNA damage induces cell-cycle arrest and apoptosis via the functions of two DNA damage response (DDR) genes, loki (also known as mnk) encoding checkpoint kinase 2 (Chk2) and p5318,19,20,21. In early oogenesis, loki/Chk2 is required for cell-cycle arrest of the germline stem cells (GSCs) and their daughters in response to DNA damage18,20. By contrast, p53 is required for cell-cycle re-entry of GSCs and their maintenance19,20. Later, in meiotic cells, p53 acts as a downstream effector of loki/Chk2 to induce apoptosis18,21. Consistent with their roles in the DNA damage response, loki/Chk2 and p53 also act as modifiers of HD-caused germline loss during oogenesis22. Although there is no known link between DNA damage response and Bruno, a regulator of oogenesis23,24,25, Bruno activity is also required for the GSC loss phenotype in adult HD females26. By contrast, at the preadult stage, the roles of loki/Chk2 and p53 in the germline-loss phenotype caused by HD remain elusive; nevertheless, germline loss is observed from embryonic stage 16 onward16,27,28. Thus, the mechanisms underlying germline elimination in HD are not fully understood.
Over the course of our experiments to screen transcription factors for germline development and its proliferation, we came across the phenotype caused by knockdown of Myc, a basic helix–loop–helix transcription factor29,30,31, that was similar to the germline-loss phenotype observed in HD progeny. In this study, we show that P-element mobilization caused downregulation of Myc in the germline. Reduction of Myc expression in the germline caused the germline-loss phenotype; conversely, overexpression of Myc restored fertility impaired by HD, resulting in production of gametes with elevated mutation frequency and reduced ability to develop into offspring. These observations strongly suggest that elimination of germline cells mediated by Myc downregulation plays an important role in maintaining the genomic integrity of the germline.
Phenotypes caused by HD and Myc knockdown
M-strain females mated with P-strain males produced HD progeny that exhibited a germline-loss phenotype14,15 (Fig. 1a–d, g). To determine the developmental stages when germline cells were eliminated, we counted the numbers of germline cells in the gonads of HD progeny. In light of the sex difference in germline cell number within a gonad, we examined males and females separately. In males, the average number of germline cells in each gonad of HD progeny embryos was almost identical to that in control embryos (Fig. 1h). However, the number began to decrease in HD progeny at the first instar, and remained low at the second instar (Fig. 1h). In females, the number of germline cells in HD progeny was equivalent to that in control animals until the first instar (Fig. 1i). The number was severely reduced in HD progeny at the third instar, although a subtle reduction of the number in HD progeny was evident at the second instar (Fig. 1i). Thus, severe reduction in the number of germline cells occurred in HD progeny at the first and third instar stages in males and females, respectively, under the conditions we used.
Over the course of our examination of the knockdown phenotypes of transcription factors, we observed that Myc knockdown caused a reduction in the number of germline cells similar to that observed in HD progeny. Myc, a transcription factor that regulates several cellular processes, including cell proliferation, growth, and death29,30,31, was knocked down by expressing a specific shRNA in the germline under the control of nos-Gal4-VP16 (nos-Gal4) driver from embryonic stage 9 onward32. When Myc was knocked down in the germline, the number of germline cells began to be reduced at the first and third instar stages in males and females, respectively (Fig. 2a, b). Similar results were obtained using another shRNA targeting a distinct region of the Myc mRNA (Supplementary Fig. 1a, b). Furthermore, Myc knockdown resulted in the germline-loss phenotype in adult ovaries and testes (Fig. 2c–g). Thus, both HD and Myc knockdown decreased germline cell number at the similar stage of development.
These observations led us to speculate that HD decreases Myc expression in the germline. To monitor Myc expression, we used green fluorescent protein (GFP)-tagged Myc protein (Myc-GFP) expressed under the control of Myc promoter, which mimics endogenous Myc expression33,34,35. In male germline, Myc-GFP was observed at embryonic stage 15, and remained detectable until spermatogenesis (Supplementary Fig. 2a, c, e, g, i, k). By contrast, in female germline, Myc-GFP was detected at embryonic stage 15, but was indiscernible at the first and early-second instar (Supplementary Fig. 2b, d, f). The signal reappeared in female germline of mid-second instar larvae, and remained detectable until oogenesis (Supplementary Fig. 2h, j, l). In HD progeny, Myc-GFP expression was significantly reduced in the nuclei of germline cells at embryonic stage 17 and the mid-second instar in males and females, respectively (Fig. 2h–m). Furthermore, the levels of Myc-GFP and endogenous Myc mRNAs were significantly reduced in the germline cells of HD males at embryonic stage 17 (Supplementary Fig. 3a–e, g), although we could not detect these mRNAs in the female germline cells at the mid-second instar, presumably because it was difficult for the probes to permeate the gonads. These results suggest that Myc expression was reduced in germline cells of HD progeny before germline number severely decreased.
Myc overexpression rescues the germline-loss phenotype in HD
The observations described above suggest that HD causes Myc downregulation in the germline, which in turn leads to the germline-loss phenotype. To address this possibility, we overexpressed Myc in the germline under the control of nos-Gal4. Although UASt is not active in the germline during oogenesis36,37, UASt-EGFP was activated by nos-Gal4 to produce EGFP protein in the germline of testes at the embryonic stage 17, and in ovaries at the mid-second instar, when Myc expression was reduced by HD (Supplementary Fig. 4). Using UASt-Myc and nos-Gal4, we overexpressed Myc in the germline cells (Supplementary Fig. 3f, g). When Myc was overexpressed in the germline cells of HD progeny (HD germline), the percentages of adult testes and ovaries without germline cells were significantly reduced relative to those in HD progeny (Fig. 1g). Conversely, the percentages of normally developed gonads were significantly higher than that in HD progeny (Fig. 1e, f and Supplementary Fig. 5). These observations indicate that Myc overexpression in germline cells rescues the germline-loss phenotype caused by HD.
Moreover, we found that Myc overexpression rescued the reduction in the number of germline cells at the first and third instar stages in HD males and females, respectively (Fig. 1h, i). Considering that both HD and Myc knockdown caused a decrease in the number of germline cells at these stages, and that Myc expression was reduced in HD progeny prior to these stages, it is reasonable to conclude that overexpression of Myc overrides Myc downregulation in the HD germline prior to these stages, which would otherwise decrease the number of germline cells and consequently the germline-loss phenotype.
Myc overexpression in HD germline produces aberrant gametes
Based on the hypothesis that Myc-downregulation-dependent reduction in the number of germline cells (MDRG) is the mechanism responsible for eliminating germline cells whose genomes have been damaged by P-element mobilization, we predicted that inhibition of MDRG by Myc overexpression in the HD germline would result in production of aberrant gametes with a high mutation frequency and a low capacity to produce progeny.
To monitor mutation frequency, we used the singed-weak (snw) allele, which exhibits a weak sn phenotype due to the insertion of two P-elements into the sn promoter locus38,39. When P-element mobilization occurs in the HD germline, the snw allele becomes highly mutable, producing either a wild-type (sn+) or extreme singed (sne) phenotype in its offspring38 (Supplementary Fig. 6). Myc overexpression in the HD male germline caused a significant increase in the percentage of the offspring with the sn+ and sne phenotypes, relative to offspring from HD males not overexpressing Myc in the germline (Fig. 3a and Supplementary Table 1). Furthermore, Myc overexpression in HD germline significantly decreased the percentage of the offspring that developed to adulthood, relative to control HD offspring not overexpressing Myc (Fig. 3b, c and Supplementary Table 2). By contrast, under non-HD conditions, neither mutation frequency nor the percentage of offspring developing to adulthood was affected by Myc overexpression (Supplementary Fig. 7 and Supplementary Tables 3 and 4). These observations indicate that inhibition of MDRG by overexpression of Myc in HD germline promotes the production of aberrant gametes that carry highly mutable genes and are incapable of developing properly to adulthood. It is unlikely that Myc overexpression merely represses mobilization of P-elements in the HD germline, because Myc overexpression in the HD germline increased mutation frequency or lethality in the offspring (Fig. 3a–c). Therefore, it is reasonable to consider that MDRG eliminates germline cells in which the genetic material has been injured by P-element mobilization.
Roles of apoptosis, p53, and loki in MDRG
Although DNA damage associated with HD is thought to cause the germline-loss phenotype, it remains unclear how DNA damage causes germline elimination17,40. DNA damage induces cellular responses, including cell-cycle arrest and apoptosis, mediated by the DDR41,42. Thus, it is possible that cell-cycle arrest and apoptosis induced by DDR are major causes of MDRG.
To address this hypothesis, we first investigated whether cell-cycle arrest was induced in HD germline at the larval stages, during which the number of germline cells is reduced by HD and Myc knockdown. Because cell-cycle arrest is inferred from reduction in the number of mitotic cells, staining for a mitotic marker, phospho-histone H3 (pH3), was performed18. The percentages of HD germline cells expressing pH3 were statistically equivalent to those in controls (Supplementary Fig. 8a, b). Furthermore, Myc overexpression did not affect the proportion of HD germline cells that expressed pH3 (Supplementary Fig. 3a, b). Together, these observations suggest that cell-cycle arrest is not a major cause of MDRG at the larval stages. Consistent with this, in males, Myc knockdown did not affect the percentage of germline cells with a pH3 signal at the first instar stage (Supplementary Fig. 8c). However, Myc knockdown decreased pH3 expression in female germline cells at the third instar. (Supplementary Fig. 8d). These results suggest that Myc downregulation did not cause cell-cycle arrest in male germline cells, but in female ones. Therefore, we cannot completely exclude the possibility that cell-cycle arrest is responsible for MDRG.
Second, we found that apoptosis was not required for elimination of germline cells caused by MDRG. Apoptosis is inhibited by overexpression of Drosophila inhibitor of apoptosis (Diap1)43. Hence, in these experiments, we used the nos-Gal4 driver to induce UASt-Diap1 in the germline prior to gametogenesis36,37. The germline-loss phenotype caused by HD and Myc knockdown was not rescued by expression of Diap1 in either sex (Supplementary Figs. 9 and 10). Therefore, it is unlikely that apoptosis is the cause of MDRG.
Finally, we sought to determine whether p53 and loki/Chk2, which are key regulators that induce cell-cycle arrest and apoptosis in the DDR44,45,46, are required for MDRG. To this end, we used p535A-1–4 and lokiP6, viable and fertile loss-of-function mutations. Supplementary Figs. 10 and 11 show that the germline-loss phenotype caused by Myc knockdown was not rescued by introduction of p535A-1–4 and lokiP6 in either sex. These observations suggest that p53 and loki/Chk2 are not essential for MDRG, although we cannot rule out the possibility that Myc is downstream of p53 and loki/Chk2. Together, these findings suggest that cell-cycle arrest and apoptosis induced by DDR through activity of loki/Chk2 and p53 are not major causes of MDRG.
In this report, we demonstrate that HD causes downregulation of Myc expression in the germline, and the Myc knockdown results in elimination of germline cells. Conversely, overexpression of Myc partially rescues germline elimination in HD larvae and adults. Moreover, the gametes produced from Myc-expressing HD germline exhibit a higher mutation frequency and a lower capacity to develop into adulthood than HD germline in the absence of Myc overexpression. These observations strongly suggest that MDRG serves to eliminate aberrant germline cells harboring DNA damage due to P-element mobilization. However, it is possible that other genes might also be involved in HD-induced germline elimination. Future studies comparing transcriptomes between non-HD and HD germline help us to identify such critical genes.
It remains unclear how MDRG is induced by HD and how MDRG leads to germline cell elimination. Because it is thought that DNA damage associated with HD causes the germline-loss phenotype17,40, one possible mechanism underlying MDRG is that Myc mediates DDR, which induces cell-cycle arrest and apoptosis in the germline41,42. In Drosophila oogenesis, when female GSCs and their daughter cells are exposed to DNA damage, loki/Chk2 is required for cell-cycle arrest of GSCs and the loss of these cells20,22. Moreover, in meiotic cells, p53 acts downstream of loki/Chk2 to induce apoptosis in response to DNA damage18,21. Thus, germline loss during oogenesis in HD females results from GSC loss and meiotic cell apoptosis, which are caused by DNA damage associated with P-element mobilization22,26. However, MDRG occurs in HD females at the early third-instar stage (Figs. 1i and 2b, k, m), when GSCs and meiotic cells have not yet been established47. Furthermore, mutations in neither loki/Chk2 nor p53 modulate MDRG (Supplementary Figs. 10 and 11). These observations suggest that MDRG is not mediated by DDR. Thus, we propose that germline elimination is regulated in females by at least two different mechanisms, one of which depends on DDR during oogenesis, and the other (MDRG) is independent of DDR at the early third-instar stage. Recent studies reported that malformation of mitochondria is induced in the germline by HD28, and mitochondrial dysfunction causes downregulation of Myc in human cell lines48. Hence, future studies focused on the role of mitochondria in MDRG might be an important step toward elucidating how Myc mediates elimination of the HD germline.
We found that Myc overexpression suppressed the germline-loss phenotype caused by HD, but the suppression was incompletely penetrant, such that approximately half of adult gonads still exhibited the germline-loss phenotype (Fig. 1g). The incomplete penetrance suggests that a pathway active in the HD germline, other than MDRG, is partly responsible for the germline-loss phenotype. Compatible with this, mutation in loki/Chk2 partially suppresses the germline-loss phenotype caused by HD22. Therefore, it is reasonable to speculate that at least two germline-elimination mechanisms, MDRG and a Chk2-dependent pathway, are responsible for the HD-induced germline-loss phenotype in females. We propose that MDRG is initiated by Myc downregulation in the germline at the larval stages, whereas DDR pathway causes GSC loss and apoptosis in early meiotic cells in response to DNA damage, as reported previously18. The reason why overexpression of Diap1 did not rescue the germline-loss phenotype in HD is that we used UASt to drive expression of Diap1, which is activated by nos-Gal4 in the germline at the embryonic and larval stages (Supplementary Fig. 4), but not during gametogenesis36,37. Thus, our UASt-Diap1 construct was unable to express Diap1 in meiotic cells, where apoptosis was evident36,37. We hypothesized that the germline cells with DNA damage are eliminated by a double-check mechanism; at the larval stages by MDRG, and later at gametogenesis by Chk2 activity.
Our observations strongly suggest that elimination of germline cells mediated by Myc downregulation maintains the germline integrity. Recent study demonstrates that higher inheritance of germ plasm correlates well with higher survival probability of primordial germ cell (PGC) in embryos49. This mechanism ensures selection of the PGCs with higher quantity of germ plasm. However, it is unlikely that this PGC selection depends on MDRG, because MDRG occurs in larvae, but not in embryos (Figs. 1g and 2a, b). We propose that MDRG acts as a quality-control mechanism in the germline, which preserves the genomic integrity of the gametes.
nos-Gal4-VP16 (nos-Gal4)32 was used to induce expression of the following UAS constructs in the germline: UASt-Myc50 (gift from Dr Orian, Dr Eisenman, and Dr Parkhurst); UASt-MycRNAi1 (36123), UASt-MycRNAi2 (51454), UASt-EGFP (6658), and UASt-Diap1 (6657) from the Bloomington Drosophila Stock Center (BDSC). To examine Myc protein expression, we used the Myc-GFP BAC transgenic strain (BDSC, 81274), which underwent recombination to generate a GFP-tagged Myc transgene within the genomic DNA surrounding the Myc locus33,34,35. To induce HD, males of the P strain Harwich (BDSC, 4264) were used. p535A-1-4 (BDSC, 6815) and lokP6 (gift from Dr Ting Xie), loss-of-function alleles of p53 and loki (Chk2), respectively, were used in the rescue experiment. To examine the mutation frequency in the germline, singed-weak (snw; 101251) and attached-X chromosome (105722) strains from the Drosophila Genomics and Genetic Resources (Kyoto stock center) were used.
Because the temperature sensitivity of HD differs between the sexes51, male and female progeny derived from HD crosses were cultured at 29 and 25 °C, respectively. Myc-knockdown flies were cultured at 30 °C. lokiP6 progeny were cultured at 25 °C, because when they were cultured at 30 °C, most of the animals died during larval and pupal development.
Immunofluorescence staining of embryos and gonads was performed as described52,53. Embryos at stage 15–17 [10 ± 2 h after egg laying (AEL) (at 29 °C) or 15 ± 3 h AEL (at at 25 °C)] were collected and dechorionated in a sodium hypochlorite solution. Gonads were dissected from early-first [23 ± 2 h AEL (at 29 or 30 °C) or 30 ± 2 h AEL (at 25 °C)], early-second [47 ± 2 h AEL (at 29 or 30 °C) or 54 ± 2 h AEL (at 25 °C)], mid-second [58 ± 2 h AEL (at 25 °C)], and early-third [71 ± 2 h AEL (at 29 or 30 °C) or 78 ± 2 h AEL (at 25 °C)] instar larvae, and adult flies 3–5 days after eclosion. The dechorionated embryos and dissected gonads were fixed in 1:1 heptane:fixative [4% paraformaldehyde in PBS (130 mM NaCl, 7 mM Na2HPO4, and 3 mM NaH2PO4)] for 30 and 15 min, respectively. Vitelline membrane of fixed embryos was removed in 1:1 methanol:heptane by vigorous shaking. The embryos and gonads were washed with PBST (0.1% Triton-X100 in PBS) three times for 15 min each, and then incubated in blocking solution (2% BSA, 0.1% Tween 20, and 0.1% Triton-X100 in PBS) for 1 h. After blocking, the embryos were incubated in the blocking solution containing the following primary antibodies overnight at 4 °C with the indicated dilutions: chick anti-Vasa54 (1:500), mouse anti-Hts [1:10; Developmental Studies Hybridoma Bank (DSHB), AB_528070], mouse anti-Fasciclin III (1:10; DSHB, AB_528238), rabbit anti-GFP (1:500; Thermo Fisher Scientific, A11122), and rabbit anti-phospho-Histone H3 (Ser10) (1:500; Merck Millipore, 06-570). Then, the embryos and gonads were washed with PBST three times for 15 min each. For detection of primary antibodies, the following secondary antibodies were used: goat anti-chick conjugated with Alexa Fluor 488 (1:500; Thermo Fisher Scientific, A11039) or Alexa Fluor 633 (1:500; Thermo Fisher Scientific, A21103), goat anti-rabbit conjugated with Alexa Fluor 488 (1:500; Thermo Fisher Scientific, A11034), and goat anti-mouse conjugated with Alexa Fluor 546 (1:500; Thermo Fisher Scientific, A11030). To enhance the Myc-GFP signal, Can Get Signal Immunostain Solution B (Toyobo, NKB-601) was used to dilute anti-GFP antibody.
To examine the signal intensity of Myc-GFP, GFP fluorescence images of the germline cells within a 20-μm range of the embryonic or ovarian upper surface were captured on confocal microscopy SP5 (Leica Microsystems) under the same laser power and gain conditions. Signal intensity of Myc-GFP in the germline nuclei was measured using the FIJI software55.
In situ hybridization
Whole-mount in situ hybridization of embryos was performed as described52,53. To synthesize RNA probes for Myc-GFP and Myc mRNA, the EGFP-coding region and Myc cDNA were amplified from pEGFP-N1 (Clontech) and an embryonic cDNA library56, respectively, using the following primer pairs: EGFP-Fw (5′-TATAGGGCGAATTGGGTACCATGGTGAGCAAGGGCGAG-3′)/EGFP-Rv (5′-TCGAGGGGGGGCCCGGTACCTTACTTGTACAGCTCGTCCATG-3′) and Myc-Fw (5′-TATAGGGCGAATTGGGTACCACCAGACACACCTCACAGTCTTAC-3′)/Myc-Rv (5′-TCGAGGGGGGGCCCGGTACCCTGCATACTAAGCTCCTTCTCCTC-3′). Amplified cDNAs were cloned into the KpnI site of pBlueScript II SK+ vector using the In-Fusion HD Cloning Kit (Clontech, 639648). Templates for RNA probes were amplified from these plasmids using the T7 and T3 primers. Digoxigenin (DIG)-labeled RNA probes were synthesized from the fragments using T7 or T3 RNA polymerase (Roche, 10881767001 and 11031163001, respectively).
Embryos at stage 17 [13 ± 1 h AEL (at 29 °C)] were collected and dechorionated in a sodium hypochlorite solution. The dechorionated embryos were fixed and devitellinized as described above. The embryos were then rinsed with ME [50 mM EGTA (pH 8.0) in 90% methanol] and incubated in 7:3, 5:5, and 3:7 ME:fixative for 5 min each. They were then incubated in fixative for 20 min and washed three times with PBSTw (0.1% Tween 20 in PBS). The washed embryos were incubated for 3 min in 50 µg/ml Proteinase K in PBSTw, and the digestion was stopped by incubation for 20 min in fixative, followed by washes three times in PBSTw for 10 min each. The re-fixed embryos were incubated in Pre-HS [50% formamide, 5 × SSC (1 × SSC is 150 mM NaCl and 15 mM sodium-citrate), 100 µg/ml heparin, 100 µg/ml yeast tRNA, 10 mM DTT, and 0.1% Tween 20] for 1 h at 60 °C, and then hybridized with 2 ng/µl of RNA probe in HS (Pre-HS containing 10% dextran sulfate) overnight at 60 °C. After hybridization, the embryos were washed six times at 60 °C for 30 min each in a solution containing 50% formamide, 5 × SSC, and 0.1% Tween 20. Signals were detected using a horseradish peroxidase-conjugated anti-DIG antibody (Roche, 11207733910), and amplified using the TSA Biotin System (PerkinElmer, NEL700001KT) and streptavidin conjugated with Alexa Fluor 546 (Thermo Fisher Scientific, S11225). In situ hybridization combined with immunostaining was performed as described53. The embryos were stained with chick anti-Vasa antibody54 (1:100). Goat anti-chick conjugated with Alexa Fluor 633 (1:500, Thermo Fisher Scientific, A21103) was used as the secondary antibody.
To examine the signal intensities of Myc-GFP and Myc mRNAs, their fluorescence images of the germline cells within a 20-μm range of the embryonic upper surface were captured on an SP5 confocal microscope (Leica Microsystems) under the same laser power and gain conditions. Signal intensities of Myc-GFP and Myc mRNAs in the germline were measured using the FIJI software46.
Examination of mutation frequency using the sn w allele
Male progeny with the snw, non-Ser, and non-Sb phenotype, derived from snw/+; nos-GAL4/TM3, Ser Sb females mated with y w males (control-snw), snw/+; nos-GAL4/TM3, Ser Sb females mated with Harwich males (HD-snw), and snw/+; nos-GAL4, UASt-Myc/TM3, Ser Sb females mated with Harwich males (HD-snw + Myc o/e), were cultured at 29 °C (Supplementary Table 1) and mated with attached-X (XX/Y) females. The offspring were allowed to develop at 25 °C. Then, adult males exhibiting singed-weak (snw), singed-extreme (sne), and wild-type (sn+) phenotypes were counted. To examine snw mutation frequency under non-HD conditions, male progeny with the snw, non-Ser, and non-Sb phenotype, derived from snw/+; nos-GAL4/TM3, Ser Sb females mated with y w males (non-HD_snw) and snw/+; nos-GAL4, UASt-Myc/TM3, Ser Sb females mated with y w males (non-HD_snw + Myc o/e), were cultured at 29 °C (Supplementary Table 3) and mated with attached-X (XX/Y) females. The offspring were allowed to develop at 25 °C, and adult males exhibiting snw, sne, and sn+ phenotypes were counted.
Determination of the rate of development to adulthood
Male and female progeny derived from nos-Gal4/nos-Gal4 females mated with y w males (control) and nos-Gal4/nos-Gal4 females mated with Harwich males (HD) were cultured at 29 and 25 °C, respectively (Supplementary Table 2). Males and females with the non-Ser and non-Sb phenotype derived from nos-GAL4, UASt-Myc/TM3, Ser Sb females mated with Harwich males (HD + Myc o/e) were cultured at 29 and 25 °C, respectively (Supplementary Table 2). These male and female progeny were mated with y w females and males, respectively. Flies were allowed to lay eggs for 16 h on grape juice plates supplemented with yeast paste at 25 °C. Eggs were collected and transferred to vials filled with a standard Drosophila medium (100 eggs/vial) and cultured at 25 °C.
The rate at which progeny developed to adulthood under non-HD conditions was examined as follows. Male and female progeny derived from nos-Gal4/nos-Gal4 females mated with y w males (control) were cultured at 29 and 25 °C, respectively (Supplementary Table 4). Males and females with the non-Ser and non-Sb phenotype derived from nos-GAL4, UASt-Myc/TM3, Ser Sb females mated with y w males (Myc o/e) were cultured at 29 and 25 °C, respectively (Supplementary Table 4). These male and female progeny were mated with y w females and males, respectively. Flies were allowed to lay eggs for 16 h on grape juice plates supplemented with yeast paste at 25 °C. Eggs were collected and transferred to vials filled with a standard Drosophila medium (100 eggs/vial) and cultured at 25 °C. The frequency of eclosion was calculated.
Statistics and reproducibility
Statistical tests conducted in each experiment are indicated in the figure legends. For all experiments, P values < 0.05 is considered to be statistically significant. Statistical significance is marked with * (P < 0.05) and ns (not significant). All attempts at replication were successful, except for Supplementary Fig. 8c. The percentage of pH3-positive germline cells was slightly downregulated (Experiment 1) and upregurated (Experiment 2) by Myc knockdown. The data combining these two results and statistical significance are shown in Supplementary Fig. 8c. The data for Myc KDRNAi1 + p53- in Supplementary Fig. 10 was obtained from a single experiment, due to a failure in maintaining Myc KDRNAi1 + p53- line. Sample sizes were determined based on published studies in this field or our published data.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
All source data underlying the bar graphs and box plot presented in the figures are reported in Supplementary Data 1. All data and materials produced by this study are available from the corresponding author upon request.
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We thank Dr Amir Orian, Dr Robert Eisenman, and Dr Susan Parkhurst for the UASt-Myc flies and Dr Ting Xie for the lokiP6 stock. We also thank the Bloomington Drosophila Stock Center and the Drosophila Genomics and Genetic Resources for providing us with fly stocks, and the Developmental Studies Hybridoma Bank for antibodies. This work was supported in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) (KAKENHI Grant Numbers: 25114002, 24247011, and 18H05552).
The authors declare no competing interests.
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Ota, R., Kobayashi, S. Myc plays an important role in Drosophila P-M hybrid dysgenesis to eliminate germline cells with genetic damage. Commun Biol 3, 185 (2020). https://doi.org/10.1038/s42003-020-0923-3
Scientific Reports (2021)