Little is known about the properties and function of ion channels that affect synaptic terminal-resting properties. One particular subthreshold-active ion channel, the Kv7 potassium channel, is highly localized to axons, but its role in regulating synaptic terminal intrinsic excitability and release is largely unexplored. Using electrophysiological recordings together with computational modeling, we found that the KV7 current was active at rest in adult hippocampal mossy fiber synaptic terminals and enhanced their membrane conductance. The current also restrained action potential-induced Ca2+ influx via N- and P/Q-type Ca2+ channels in boutons. This was associated with a substantial reduction in the spike half-width and afterdepolarization following presynaptic spikes. Further, by constraining spike-induced Ca2+ influx, the presynaptic KV7 current decreased neurotransmission onto CA3 pyramidal neurons and short-term synaptic plasticity at the mossy fiber–CA3 synapse. This is a distinctive mechanism by which KV7 channels influence hippocampal neuronal excitability and synaptic plasticity.
Neurotransmitter release from synaptic terminals is the predominant mechanism for information transfer between neurons and has a fundamental role in processes such as synaptic plasticity1,2,3. Diverse synaptic terminals express the high voltage-activated KV1 and KV3 channels that are activated during the repolarization phase of the presynaptic action potential. These K+ channels regulate the action potential width and, consequently, presynaptic Ca2+ influx and neurotransmission4,5,6,7,8,9,10,11,12,13,14,15,16. There are, though, K+ channels that activate at rest (i.e., at subthreshold potentials)17. Much less is known about the properties and function of these channels in synaptic terminals.
The KV7 channels activate at voltages positive to − 70 mV in many neurons and form an inhibitory current at rest17,18,19. There are 5 KV7 subunits, of which KV7.2–KV7.5 subunits are neuronal. The KV7.2 and KV7.3 subunits contain an ankyrin G-binding motif, so they are expressed at the axon initial segment and nodes of Ranvier where they regulate the action potential threshold and propagation17,18,20,21,22,23. Interestingly, KV7.5 channels are present in the giant synaptic terminals of Calyx of Held, where they influence the resting membrane potential (RMP)24. Immunohistochemical evidence also suggests that KV7.5 subunits are situated in GABAergic, but not glutamatergic, synaptic terminals in the hippocampus25. In contrast, immunohistochemistry suggests that KV7.2 subunits are expressed throughout hippocampal mossy fibers26,27,28. However, it is not known if hippocampal mossy fiber boutons have a KV7 current or whether this affects their local intrinsic excitability and neurotransmitter release. It is important to investigate this as the mossy fiber giant bouton-CA3 pyramid synapse has a critical role in processes such as learning and memory and in pathophysiological disorders such as epilepsy29,30,31,32,33.
Here, we show that adult mossy fiber boutons possess a KV7 current that is active at rest. This had little effect on the bouton RMP. The current, though, altered the intrinsic excitability of boutons by decreasing the membrane resistance at voltages positive to rest. The current also limited action potential-induced Ca2+ influx via voltage-gated N- and P/Q-type Ca2+ channels. This was coupled with a reduction in the presynaptic spike half-width and an afterdepolarization that follows presynaptic spikes in these boutons. Further, by limiting action potential-induced Ca2+ influx, KV7 channels restricted neurotransmitter release and short-term synaptic plasticity onto CA3 pyramidal neurons. This is a unique mechanism by which presynaptic KV7 channels affect local excitability within adult hippocampal synaptic terminals and regulate neurotransmission. This might be an important means by which KV7 channels contribute towards influencing neural network rhythms and maintaining network excitability in the hippocampus34,35,36.
KV7 currents in mature mossy fiber boutons
As mossy fiber boutons that synapse onto CA3 proximal apical dendrites have large diameters (2–5 μm, Fig. 1a)10,31,32,37, we made electrophysiological recordings from these present in hippocampal slices obtained from mature rats. The tracer, neurobiotin, was included and post-hoc morphological analysis was performed to positively identify boutons (Fig. 1a). To record the KV7 current, the classical de-activation protocol38,39,40 was applied under whole-cell voltage-clamp condition in the absence and presence of the specific, irreversible, pharmacological KV7 channel inhibitor, XE991 (3 μm, a concentration that inhibits > 95% of the current41) (Fig. 1b). This revealed slow de-activating currents that reversed at ~ − 90 mV (i.e., near the K+ reversal potential, Fig. 1b). The currents were stable for at least 20 min, with minimal rundown and were maximally inhibited by 20 min bath application of XE991 (Fig. 1b), suggesting that the KV7 current was present in mossy fiber boutons.
To determine whether the KV7 currents recorded under whole-cell voltage-clamp conditions were generated, at least partly, in the bouton per se, we made outside-out patch recordings from the boutons (Fig. 1c). Slow de-activating currents with comparable properties to those obtained under whole-cell voltage-clamp experiments were present in outside-out patches too. These were fully inhibited by XE991 (Fig. 1c). The half voltage-activation (V1/2) and slopes of the activation curves from whole-cell and outside-out patch configurations were similar (whole-cell and outside-out patch V1/2 = −69.6 ± 1.6 mV (n = 6) and −73.9 ± 1.8 mV (n = 5), respectively; whole-cell and outside-out patch activation curve slopes = 5.5 ± 1.5 (n = 6) and 7.6 ± 1.7 (n = 5), respectively; Fig. 1d; see Methods). The current density and de-activation time constants measured at − 50 mV were comparable in both configurations (Fig. 1e, f). Given the remarkable similarities in KV7 current densities and biophysical properties between outside-out patch and whole-cell voltage-clamp configurations, our results strongly suggest that KV7 channels are located on mature mossy fiber boutons synapsing onto CA3 pyramidal neurons.
KV7 currents limit the membrane resistance and excitability
To ascertain whether KV7 channels affect mossy fiber bouton intrinsic excitability, we made whole-cell current-clamp recordings from boutons in the presence of glutamate and GABA receptor blockers (see Methods) in the absence and presence of XE991 (3 μM). The boutons had an average RMP of − 81.9 ± 1.2 mV (n = 8), which was unaffected by 20 min bath application of XE991 (average RMP with XE991 = −81.7 ± 1.3 mV (n = 8, p = 0.83, two-tailed paired t test); Fig. 2a, b). Similar findings were also obtained with 20 min application of a second KV7 channel inhibitor, linopirdine (10 μM)41 (Fig. 2b). Given that our data (Fig. 1d) suggests that ~ 20% of the KV7 current is active at −80 mV, this implies that other ion channels, such as the inward rectifier potassium channels42,43 and twin-pore potassium channels, have a larger influence on the RMP in mossy fiber boutons.
Depolarizing current pulses of varying magnitudes resulted in only single action potentials in boutons (Fig. 2a). The inability of more than one spike to be initiated with different magnitudes and sustained depolarization is probably owing to the specialized properties of the mossy fiber bouton Na+ and other K+ (e.g., KV1) channels14,44. In the presence of either XE991 or linopirdine, though, significantly less depolarizing current was required for action potential initiation (Fig. 2a–c). Thus, KV7 channels enhance the rheobase for spike generation in mossy fiber boutons.
If KV7 channels do not affect the RMP in mossy fiber boutons, how might they affect the ability for depolarizing pulses to result in spike generation here? In many other neurons, the inhibitory current formed by KV7 channels at subthreshold potentials limits the amount of depolarization with given current injections (i.e., decreases the input resistance (RN)), resulting in a larger rheobase required to elicit action potentials. Thus, we examined whether these channels modified RN in mossy fiber boutons too. To test this, we injected small hyperpolarizing and depolarizing subthreshold current pulses at a fixed potential of − 80 mV (i.e., near the bouton RMP) in the absence and presence of XE991 or linopirdine (Fig. 2d). RN measured at potentials above −80 mV, but not at hyperpolarizing potentials, was significantly greater in the presence of XE991 or linopirdine (Fig. 2d, e). This effect was time-dependent, peaking ~20 min after application of XE991 (Fig. 2f). As mossy fiber boutons are electronically compact14,45, the effect on RN is likely to be owing to local KV7 channels in the mossy fiber bouton. Indeed, in the granule cell somata where KV7 channels are not present, KV7 channel inhibitors have little effect on RN40. Hence, in mossy fiber boutons, KV7 channels generate an inhibitory current that restricts RN at positive potentials to rest and thereby, limits the number of presynaptic spikes elicited by depolarization.
KV7 currents reduce spike width and afterdepolarization
In most neurons, single or trains of spikes invade synaptic terminals, leading to neurotransmitter release1,2,3. Thus, we injected very short current pulses (0.1 ms) to evoke single or trains of action potentials at various frequencies (1, 5, 20, 50, and 100 Hz; Fig. 3). Each spike was followed by a small intrinsically generated afterdepolarization (ADP) (Fig. 3a). This ADP duration was ≥ 150 ms, such that with spike trains generated at ≥ 20 Hz, the spikes did not initiate during the ADP (Fig. 3a). Inhibition of KV7 currents by XE991 or linopirdine enhanced the ADP amplitude and decay time constant following a single action potential to a similar extent irrespective of the frequency of the spike train or the position of the action potential within the train (Fig. 3b; Supplementary Table 1). The spike width in the presence of either XE991 or linopirdine was also broader than under control conditions (Fig. 3c). Further, the spike amplitudes were smaller in the presence of KV7 channel inhibitors compared with controls (Fig. 3c; Supplementary Table 2).
At frequencies of ≤ 20 Hz, under control conditions, each subsequent spike in a train occurred during the ADP generated by the preceding action potential, resulting in a summating ADP (Fig. 3d, e). The area under the ADP was much greater after application of XE991 or linopirdine than under control conditions (Fig. 3d, e). As KV7 channel inhibition at the granule cell axon initial segment did not result in enhancement of an ADP40,46, it suggests that these channels differentially regulate intrinsic excitability in granule cell axon subcompartments.
The KV7 conductance limits spike-induced Ca2+ concentration
To further understand the cellular mechanisms by which KV7 channels might restrict the spike width and the ADP amplitude following spikes, we generated a single compartment model consisting predominantly of the KV7 conductance with our biophysical characteristics (Fig. 1), an inward rectifier type K+ conductance, the ‘A’-type (KV1) conductance, delayed rectified type K+ conductance, Na+ conductance and a Ca2+ conductance (see Supp. Table 3 and Methods). The RMP under these conditions was −79.1 and −78.6 mV upon removal of the KV7 conductance. This small (0.5 mV) depolarization caused by loss of the KV7 conductance is within experimental error and would not have been detected in experiments. Ablation of the KV7 conductance, though, enhanced the RN from 1.00 GΩ to 2.33 GΩ when measured using depolarizing subthreshold current pulses (Fig. 4a). These findings are consistent with experimental observations (Fig. 2).
Next, we simulated an action potential with and without the KV7 conductance (Fig. 4b). In agreement with our experimental observations (Fig. 3, Supplementary Table 2), the spike width broadened from 0.55 ms to 0.75 ms upon removal of the KV7 conductance. In the absence of the KV7 conductance (Fig. 4b), the spike amplitude was higher than controls. The model suggested that a reduction in spike amplitude, consistent with experiments, could be produced by reducing the Na+ conductance by 20% (Supplementary Fig. 1).
We also modeled the changes in Ca2+ concentration induced by a single spike (Fig. 4c). Consistent with previous studies47,48,49, the simulations predicted that with a KV7 conductance (i.e., under control conditions), an action potential caused the intracellular Ca2+ concentration to increase by 100 nM (Fig. 4c). Removal of the KV7 conductance induced a further rise in intracellular Ca2+ by 60 nM (Fig. 4c). Given that the Ca2+ conductance is activated during a spike50,51, the model suggests that the rise in intracellular Ca2+ during the spike would have contributed to the spike broadening in the absence of the KV7 conductance. Indeed, the spike width in our simulations without the KV7 and Ca2+ conductance was very similar to control conditions (i.e., when both conductances were active; Fig. 4b).
Removing the KV7 conductance also resulted in the generation of an ADP with a decay time constant of 22.9 ms (Fig. 4b). In agreement with our experimental observations, a train of 20 action potentials at a frequency of 50 Hz resulted in successive action potentials being initiated near the peak of the ADP generated by the previous spike (Fig. 4d). The enhanced ADP following spikes in the absence of the KV7 conductance was abolished when the Ca2+ conductance was removed (Fig. 4b, d). These findings imply that the KV7 current in boutons serves to suppress a rise in intracellular Ca2+ concentration during an action potential and regulates the spike width and generation of an ADP succeeding the presynaptic spike.
BAPTA prevents KV7 inhibitor effects on spike with and ADP
To test whether the effects of KV7 channel inhibition on the spike half-width and ADP is owing to a rise in intracellular Ca2+, we included the Ca2+ chelator, BAPTA (10 mM or 20 mM) in the intracellular patch pipette (see Methods). The findings with 10 mM and 20 mM BAPTA were no different and have been grouped together. Full dialysis of BAPTA into boutons occurred within 5 min. Stable recordings could be obtained for at least 30 min with BAPTA in the intracellular solution. Under these conditions, 20 min application of XE991 had little effect on the single action potential half-width or amplitude (Fig. 5a, Supplementary Table 1).
In addition, XE991 had little effect on the ADP amplitude or decay time constant elicited by single action potentials at either 1 or 5 Hz when BAPTA was included in the patch pipette (Fig. 5, Supplementary Table 1). This was independent of the spike frequency or the position of the spike within the train (Supplementary Table 1). Further, there was no increase in the ADP generated during a train of action potentials elicited at frequencies of 20, 50, or 100 Hz in the presence of XE991 when BAPTA was present in the patch pipette solution (Fig. 5). Thus, these findings further support the suggestion that KV7 channel inhibition in mossy fiber boutons induces a rise in intracellular Ca2+ that leads to spike broadening and an enhanced ADP following presynaptic spikes.
KV7 currents limit Ca2+ influx to affect spike width and ADP
Next, we asked what might be the source of the rise in intracellular Ca2+ in the absence of KV7 channels? Mossy fiber boutons express predominantly P/Q-type voltage-gated Ca2+ channels as well as N- and R-type Ca2+ channels51. The N- and P/Q-type channels are activated during presynaptic spikes in mossy fiber boutons51. We, therefore, investigated whether Ca2+ influx via these channels might contribute towards the spike broadening and increase in ADP amplitude and decay time constant caused by KV7 channel inhibition. For this, we tested the effects of co-application of 3 μm XE991 and the selective N- and P/Q-type Ca2+ channel inhibitors, 100 nm ω-conotoxin GVIA and 100 nM ω-agatoxin IVA. With these inhibitors, the RN measured by applying depolarizing, but not hyperpolarizing, subthreshold steps was still enhanced compared with that prior to application of the compounds (Fig. 6a).
We then elicited trains of action potentials at different frequencies in the absence and presence of XE991, ω-conotoxin GVIA and ω-agatoxin IVA. Whereas the spike amplitude was still significantly reduced by application of the inhibitors (Fig. 6b, c, Supplementary Table 2), there was little difference in spike width with and without the inhibitors (Fig. 6b, c, Supplementary Table 2). Further, we found that the ADP amplitude and decay time constant following spikes occurring at 1 Hz and 5 Hz was significantly reduced in the presence of XE991 and N- and P/Q-type Ca2+ channel inhibitors compared with that controls (Fig. 6b, c, Supplementary Table 1).
As previously found (Fig. 3d), action potential trains at ≤ 20 Hz resulted in spikes being generated on the ADP under control conditions (Fig. 6d). Co-application of XE991 and N- and P/Q-type Ca2+ channel inhibitors resulted in a reduction of the ADP area compared with control conditions such that spikes now occurred at the normal RMP (i.e., at the baseline; Fig. 6d, e). Altogether, these findings robustly support the notion that the KV7 current reduces Ca2+ influx via N- type and P/Q-type Ca2+ channels and, thereby, regulates the spike width and ADP generated following spikes in mossy fiber boutons.
KV7 channels reduce neurotransmission onto CA3 neurons
Given that our findings suggest that KV7 channels limit Ca2+ influx via N- and P/Q-type Ca2+ channels, which regulate synaptic release from mossy fiber boutons10,51,52,53, we hypothesized that KV7 channel inhibition should enhance neurotransmitter release from mossy fiber boutons onto CA3 pyramidal neurons. To investigate this, we obtained cell-attached recordings from a mossy fiber bouton and whole-cell voltage-clamp recordings from the CA3 pyramidal neuron whose proximal apical dendrite the bouton was next to in the absence of glutamate and GABA receptor blockers (see Fig. 7a45). As CA3 pyramidal neurons express KV7 channels postsynaptically54, we replaced intracellular K+ in the CA3 pyramidal neuron with Cs+ to inhibit these channels (see Methods). We also included QX314 bromide in the CA3 pyramidal recording solution to inhibit Na+ channels. As dentate–gyrus granule cells are most likely to fire action potentials phase-locked to theta or gamma rhythms in vivo55, we initially induced action currents at 5 Hz (i.e., theta frequency) in the bouton as in ref. 56 (Fig. 7a). This resulted in mono-synaptic excitatory post-synaptic currents (EPSCs) in the post-synaptic CA3 neuron with a very low failure rate (% failure = 8.33 ± 10.2%, n = 3, Fig. 7b). Subsequent application of XE991 (3 μm) enhanced the EPSC amplitude and decay time constant (Fig. 7a, b), strongly suggesting that presynaptic KV7 channels constrain neurotransmitter release from mossy fiber boutons.
As spike trains in granule cells at gamma frequency have a higher probability of initiating action potentials in CA3 pyramidal neurons during certain types of behavior45,57, we also investigated how presynaptic KV7 channels would affect neurotransmitter release when the spike frequency was 50 Hz. For this, we elicited 10 action currents at 50 Hz in boutons (Fig. 7c). As has been previously described45, this resulted in paired pulse facilitation of EPSCs (Fig. 7c, d). Bath application of XE991 significantly reduced the paired pulse ratio of evoked EPSCs (Fig. 7c, d), indicating that presynaptic KV7 channels restrict neurotransmitter release from mossy fiber boutons independently of spike frequency. Further, these findings robustly suggest that presynaptic KV7 channels affect short-term synaptic plasticity at this hippocampal synapse.
Here, we show that mossy fiber boutons possess a KV7 current that was active at rest in these structures (Fig. 1). Despite this, electrophysiological recordings and computational modeling indicated that the current had little effect on bouton RMP (Fig. 2, Fig. 4). Electrophysiological recordings and computational modeling, though, showed that the KV7 current acts as a shunt: restricting RN at depolarizing potentials to rest (Figs. 2 and 4). Consequently, KV7 currents influenced the probability of spikes elicited with a given depolarization (Fig. 2). Further, KV7 currents were found to restrict the spike width and ADP amplitude and decay following a spike (Fig. 3). Computational modeling suggested that this was due to KV7 currents opposing Ca2+ influx via N- and P/Q-type Ca2+ channels during an action potential (Fig. 4). Consistent with this, the presence of either the Ca2+ chelator, BAPTA, or the N- and P/Q-type Ca2+ channel inhibitors prevented the spike width broadening and the increase in ADP after KV7 current inhibition (Figs. 5 and 6). Moreover, in agreement with our premise that KV7 currents restrict spike-induced Ca2+ influx in mossy fiber boutons, this current significantly reduced action potential-induced neurotransmitter release from mossy fiber boutons and short-term synaptic plasticity at the mossy fiber bouton–CA3 synapse (Fig. 7). These findings indicate that KV7 channels play a substantial role in modulating intrinsic excitability and synaptic plasticity at mature synapses in the hippocampus. Our findings also imply that KV7 channels are likely to be involved in processes underpinning information storage in the hippocampus.
The mossy fiber bouton KV7 current half–maximal activation voltage was more negative (−70 mV, Fig. 1d) than that in hippocampal and neocortical pyramidal neuron somata and axon initial segments39,58 but consistent with that at granule cell somata40, in peripheral axons59 and in Calyx of Held terminals24. Hence, ~ 20% of the current was active at rest in mossy fiber bouton terminals. The lack of effect of the current on the bouton RMP is probably owing to the inward rectifier K+ current as this has been shown to exert a strong influence on RMP in granule cells42,43,60. Unlike in heterologous systems61, though, KV7 channel inhibitors exert their effects at negative potentials in native cells as they depolarized the RMP and enhanced RN in hippocampal and neocortical pyramidal neurons18,24,39,58,62,63. Further, axonal KV7 currents in peripheral nerve fibers have a XE991-sensitive component at ~ −70 mV59. In mossy fiber boutons, consistent with our computational model (Fig. 4a), XE991 increased RN measured using small subthreshold depolarizing pulses in a time-dependent manner too (Fig. 2f).
KV7 currents affect mossy fiber bouton terminal excitability distinctly from that of Calyx of Held terminals24. In particular, unlike in Calyx of Held terminals, KV7 currents regulate the spike width and ADP following spikes in mossy fiber boutons. These effects are unlikely to be due to non-selective effects of KV7 channel inhibitors as, at the concentrations utilized, XE991 and linopirdine were at least 50-fold and three fold more potent, respectively, for KV7 than other K+ channels64.
KV7 currents also restrict the generation of an intrinsic ADP succeeding spikes in CA1, CA3 and cortical pyramidal neurons58,62,65,66,67. In CA1 and cortical pyramidal neurons, KV7 currents counteract a persistent Na+ current to reduce the ADP following spikes58,62,65. In these neurons, R-type Ca2+ tail currents68 and Ca2+-activated cation channels such as TRPC channels activated via G-protein coupled receptors40,69,70,71 can contribute to the ADP following spikes too. However, whilst hippocampal granule cell somata and dendrites highly express TRPC subunits, immunohistochemistry suggested that TRPC subunits are present intracellularly within mossy fiber axons and boutons72. We cannot, though, exclude the possibility of Ca2+-activated conductances underlying the ADP generated by KV7 current inhibition. Since we do not know if these are present in mossy fiber terminals or their biophysical properties, we were unable to include them in our computational model (see Methods and Supplementary Table 3).
Our computational model included a Ca2+ conductance whose decay time constants were slowed down to mimic the Ca2+ transient generated in response to a single action potential in a mossy fiber terminal obtained using Ca2+ imaging47,48,49. The decay time constant of the Ca2+ conductance was considerably slower than that reported for either N-, P/Q-or R-type Ca2+ currents in mossy fiber terminals51 and may reflect processes such as Ca2+-induced Ca2+ release48 or the effects of endogenous buffers on intracellular Ca2+ 47. The presence of this Ca2+ conductance resulted in an ADP following KV7 conductance removal in our simulations (Fig. 4), albeit smaller and faster than that observed following single action potentials under our experimental conditions (Fig. 3). This suggests that, as in CA1 pyramidal neurons68, a Ca2+ tail current as well as processes such as Ca2+-induced Ca2+ release may contribute to the ADP generation following KV7 current suppression. However, as the simulated ADP amplitude and decay are clearly different from that observed experimentally (Fig. 3), other conductances, such as Ca2+-activated conductances, may also contribute to the KV7 current inhibition-induced ADP in terminals.
The main physiological role of presynaptic KV7 channels in mossy fiber boutons is to regulate synaptic transmission onto CA3 pyramidal neurons (Fig. 7). As spike broadening has been associated with enhanced neurotransmission from synaptic terminals5,7,14,73 and KV7 current inhibition results in wider action potentials in mossy fiber boutons (Fig. 3), this may have contributed to the greater action potential-induced neurotransmitter release in the absence of KV7 currents (Fig. 7). Further, the enhanced ADP resulting from KV7 current inhibition is probably due to augmented Ca2+ influx and may result in elevated asynchronous release from these terminals. This, though, needs to be further tested. Notwithstanding, the mechanisms by which a reduction in KV7 currents in mossy fiber boutons might lead to greater neurotransmitter release differs from that in Calyx of Held terminals, whereby RMP depolarization was largely attributed to the increase in neurotransmission following KV7 current block31. The effect of KV7 currents on intrinsic excitability and synaptic transmission may also vary in different conditions. Indeed, during elevated extracellular K+ conditions when axons are already depolarized, these currents in CA3 axons have been suggested to influence Na+ current inactivation and increase presynaptic action potential amplitude, Ca2+ influx and enhance synaptic release66. Thus, KV7 currents may have synapse-specific effects on intrinsic excitability which may depend on particular conditions.
Given that KV7 currents in mossy fiber boutons significantly augmented the membrane conductance (Figs. 2 and 4), it is probable that, like in Calyx of Held terminals24, the current will affect excitatory synaptic potential amplitudes and shapes (i.e., analog signaling) in these terminals. As analog signaling in mossy fiber boutons influences neurotransmission73, this might be another mechanism by which KV7 currents might regulate synaptic release. Hence, KV7 currents might affect neurotransmission by multiple mechanisms, including action potential-dependent release (Fig. 7).
What effect might modulation of neurotransmitter release by KV7 channels have on CA3 neural network excitability? Our findings suggest that KV7 channels restricts neurotransmitter release from mossy fiber boutons elicited by trains of action potentials occurring at theta and gamma frequencies and will thereby, contribute to maintaining CA3 pyramidal neuronal excitability (Fig. 6). Although the giant mossy fiber bouton–CA3 pyramidal neuron connectivity is sparse (with ~ 50 granule cells contacting one CA3 neuron), each bouton contains an average of 20 release sites33,74,75. Thus, changes in KV7 channel activity could have an impact on the overall CA3 pyramidal neuron excitability. Indeed, a loss of KV7.2 subunits, which are present on mossy fibers and, most likely, their boutons26,27,28, led to impaired hippocampal gamma rhythms and spatial learning as well as spontaneous seizures in rodents, implicating hyperexcitability of cortical neural networks35. Thus, the presence of these channels at the mossy fiber bouton, which is a conditional detonator synapse, is likely to be vital for preventing CA3 neuronal and neural circuit hyperactivity. Further, as the KV7 current significantly affects short-term synaptic plasticity at the mossy fiber–CA3 synapse (Fig. 6), these channels at this synapse may also affect long-term synaptic plasticity and thus, memory encoding.
Acute slice preparation
The UK Home Office approved all procedures. Hippocampal slices were prepared as described in76. In brief, 22–28 day-old male Sprague Dawley rat pups were decapitated, the brain removed and submerged in ice-cold solution (mM): 87 NaCl, 25 NaHCO3, 10 glucose, 75 sucrose, 2.5 KCl, 1.25 NaH2PO4, 0.5 CaCl2, 7 MgCl2, pH 7.3, 325 mOsm/L. The brain was hemi-sected and a cut parallel to the dorsal part of the brain made. The dorsal side brain halves were glued onto a slice holder and 300–400 μm slices made (Leica VT1200S, Leica, UK). Slices were incubated in the cutting solution for 30–40 min at 35 °C and then stored in the cutting solution at room temperature.
Slices were transferred to a submerged chamber containing external solution (mm): 125 NaCl, 25 NaHCO3, 25 glucose, 2.5 KCl, 1.25 NaH2PO4, 2 CaCl2, 1 MgCl2, 0.05 CNQX, 0.05 DL-AP5, 0.01 bicuculline, 0.001 CGP 55845, pH 7.3, 32–36 °C. For whole-cell current-clamp recordings, the internal pipette solution contained (mm): 120 KMeSO4, 15 KCl, 10 HEPES, 2 MgCl2, 0.2 EGTA, 2 Na2ATP, 0.3 Tris-GTP and 14 Tris-phosphocreatinine, pH 7.3 with KOH, 295–300 mOsm/L. In some experiments, 10 or 20 mM K4BAPTA was added to the pipette solution. In this case, the KMeSO4 was reduced accordingly to 50 or 60 mm, respectively and osmolarity adjusted by adding N-methyl-d-glucamine. Pipettes had resistances of 5–8 MΩ. In all experiments, Neurobiotin (0.2% w/v) was included in the intracellular pipette solution. Slices were fixed in 4% paraformaldehyde and stained with streptavidin Alexa Fluor 488 conjugate (0.04 mg/ml) 24 h later77.
Electrophysiological recordings were made using a Multiclamp 700B amplifier (Molecular Devices, UK). Current-clamp recordings were filtered at 10 kHz, and sampled at 50 kHz. Protocols (including that of RN (Fig. 2), action potentials initiated with depolarizing steps (Fig. 2) and trains of action potentials (Fig. 3) were applied every minute after application of KV7 current inhibitors to facilitate their effects during these recordings61. Data were acquired using pClamp 10.0 (Molecular Devices, UK). Series resistance was in the order of 10–30 MΩ. Recordings were discarded if the series resistance increased by > 20%.
KV7 current recordings: The external solution was supplemented with 0.001 mm tetrodotoxin and 0.1 mm 4-aminopyridine. The internal solution described above was present in the patch pipette. For outside-out recordings, the whole-cell configuration was first obtained and the patch pipette slowly withdrawn. Series resistance was between 10 and 20 MΩ and was ~ 70% compensated. A de-activation protocol (as described in ref. 39; Fig. 1b) was applied in the absence and presence of the KV7/M–channel blocker, XE991 (3 μm). Recordings were filtered at 1 kHz and sampled at 10 kHz.
Paired bouton–CA3 recordings: Cell-attached recordings from mossy fiber boutons were obtained. The internal solution was as described above. Action currents were elicited in the cell-attached mode by applying 800 mV, 0.1 ms pulses. The internal solution for CA3 neuron whole-cell recordings contained (mm): 135 CsCl, 5 QX314 bromide, 10 HEPES, 2 MgCl2, 0.2 EGTA, 2 Na2ATP, 0.3 Tris-GTP and 14 Tris-phosphocreatinine, pH 7.3 with CsOH, 295–300 mOsm/L. Glutamate and GABA receptor blockers were omitted from the external solution. Voltage-clamp recordings were obtained from CA3 pyramidal cells using a Multiclamp 700B amplifier (Molecular Devices, UK). Recordings were filtered at 1 kHz, and sampled at 10 kHz. Post-synaptic series resistance was in the order of 10–20 MΩ. Recordings were discarded if the series resistance increased by > 20%.
All reagents were purchased from Sigma-Aldrich UK apart from tetrodotoxin, bicuculline, CGP 55845, DL-AP5 and XE991, which were obtained from Abcam Ltd (UK). Neurobiotin was acquired from Vector Laboratories Ltd and streptavidin Alexa Fluor 488 was procured from Life Technologies.
Clampfit (v10.4 or v10.7) was used. To calculate RN, the difference in steady-state voltage in the last 25 ms elicited by 1 s hyperpolarizing step at − 80 mV was divided by the applied current. Action potentials elicited by 1 s depolarizing steps were counted. Action potential height was measured from threshold to the peak, whereas action potential width was the breadth at half the height. The amplitude and decay time constant of the ADP following single action potentials evoked at 1 or 5 Hz were also measured. To obtain the decay time constant, the decay phase of the ADP was fitted with a double exponential function:
where τ1 and τ2 represent time constants of the initial and falling phase of the ADP. τ2 values have been reported in Results and Figures. In addition, the area under the ADP generated during and following a train of action potentials at 20, 50, and 100 Hz was measured before and after application of XE991 (3 μm). There was some variability (albeit non-significant) in these parameters between control recordings from individual mossy fiber boutons (see Fig. 3, Fig. 5, Supplementary Table 1).
For paired bouton–CA3 recordings, the amplitude of the EPSCs generated in response to the action current in the bouton was measured. Both first and second EPSC amplitudes were measured from their directly preceding baselines, respectively. The 10–90% rise time of the EPSC was obtained in Clampfit 10.4 using the function:
Where A is the peak amplitude of the EPSC and t1 and t2 are the times at 10% and 90% of A, respectively. The decay phase of the EPSC was fitted with the above double exponential equation with τ1 and τ2 represent time constants of the initial and falling phase of the EPSC. Again only τ2 values are reported. Paired pulse ratios were calculated as the peak of the second EPSC divided by the peak of the first EPSC.
For KV7/M current voltage-clamp data, the traces obtained in the presence of the XE991 (3 μm) were subtracted from those in the absence. The subtracted traces were fitted with the above double exponential function with τ1 and τ2, representing the de-activation time constants of the initial and late phase of the KV7 current.
The KV7 conductance values were generated from the normalized amplitudes of the subtracted currents39. For whole-cell voltage-clamp experiments, the absolute voltage recorded was subtracted from the estimated reversal potential of K+ (EK). This together with the current amplitude recorded was used to calculate the conductance and were plotted against the absolute voltage. The curves were fitted using the Boltzmann equation:
where A1 and A2 are the initial and maximum values, x0 is the half-activation voltage and dx is the slope of the curve.
Group data are expressed as mean ± SEM. In all experiments, a minimum of three brain slice preparations made from three independent animals were used. For experiments involving pharmacological drug application (i.e., XE991, linopirdine or N- and P/Q-type voltage-gated Ca2+ channel inhibitors), paired t tests were used with statistical significance determined to be p < 0.05. Significant differences at p < 0.05 is indicated as asterisks (*) in all figures.
All simulations were carried out using the NEURON simulation environment (v7.5)78. All model and simulation files will be uploaded to the ModelDB database (https://senselab.med.yale.edu/modeldb/ accession no. 245417). The mossy fiber synaptic bouton was modeled as a single compartment (length = 3.5 μm, diameter = 2 μm, Cm = 1 μF/cm2, Rm = 30 kΩ/cm2, Ra = 150 Ω cm). Temperature was set at 34 °C. Active properties included a transient Na+ conductance, four types of K+ currents (delayed rectifier type K+ conductance, A-type K+ conductance, KV7 conductance, and inward rectifier type K+ conductance), a Ca2+ conductance (which is owing to all Ca2+ conductances in the bouton including N-, P/Q- and R-type Ca2+ conductances), and a simple Ca2+-extrusion mechanism with a 500 ms time constant, which is consistent with that reported by other studies47,48,49. Kinetics for the delayed rectifier type K+ conductance, A-type K+ conductance and Ca2+ conductance were taken from a previously published model39 (ModelDB accession no. 112546); the Na+ kinetics was implemented as in ref. 44; the inward rectifier K+ conductance was implemented as in ref. 60 (downloaded from ModelDB, accession no. 185355). The peak conductances used in all simulations and kinetic parameters modified with respect to their original values are reported in Supplementary Table 3. Increasing the peak conductance of the delayed rectifier conductance had little effect on the simulations. The Ca2+ conductance peak value and decay time constant were adjusted to reflect Ca2+ transient measured in response to an action potential in a mossy fiber bouton. The effects of XE991 application were modeled with a complete block of the KV7 conductance. A single action potential was elicited with a current pulse of 30 pA for 0.35 ms. To simulate a 50 Hz stimulation, the model was stimulated every 20 ms with 30 pA 0.3 ms long current pulses. Input resistance was measured from the voltage deflection caused by a 1 s long 0.3 pA current injection.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
All experimental data generated or analyzed during this study are included in this article, supplementary information files, and is available from the authors upon reasonable request.
The complete set of files for the computational model are available on ModelDB (https://senselab.med.yale.edu/modeldb/ accession no. 245417).
Schneggenburger, R. & Rosenmund, C. Molecular mechanisms governing Ca2+ regulation of evoked and spontaneous release. Nat. Neurosci. 18, 935–941 (2015).
Kaeser, P. S. & Regehr, W. G. Molecular mechanisms for synchronous, asynchronous, and spontaneous neurotransmitter release. Annu. Rev. Physiol. 76, 333–363 (2014).
Kavalali, E. T. The mechanisms and functions of spontaneous neurotransmitter release. Nat. Rev. Neurosci. 16, 5–16 (2015).
Trimmer, J. S. Subcellular localization of K+ channels in mammalian brain neurons: remarkable precision in the midst of extraordinary complexity. Neuron 85, 238–256 (2015).
Hoppa, M. B., Gouzer, G., Armbruster, M. & Ryan, T. A. Control and plasticity of the presynaptic action potential waveform at small CNS nerve terminals. Neuron 84, 778–789 (2014).
Foust, A. J., Yu, Y., Popovic, M., Zecevic, D. & McCormick, D. A. Somatic membrane potential and Kv1 channels control spike repolarization in cortical axon collaterals and presynaptic boutons. J. Neurosci. 31, 15490–15498 (2011).
Begum, R., Bakiri, Y., Volynski, K. E. & Kullmann, D. M. Action potential broadening in a presynaptic channelopathy. Nat. Commun. 7, 12102 (2016).
Nakamura, Y. & Takahashi, T. Developmental changes in potassium currents at the rat calyx of Held presynaptic terminal. J. Physiol. 581, 1101–1112 (2007).
Ishikawa, T. et al. Distinct roles of Kv1 and Kv3 potassium channels at the calyx of Held presynaptic terminal. J Neurosci. 23, 10445–10453 (2003).
Bischofberger, J., Engel, D., Frotscher, M. & Jonas, P. Timing and efficacy of transmitter release at mossy fiber synapses in the hippocampal network. Pflugers Arch. 453, 361–372 (2006).
Dodson, P. D. et al. Presynaptic rat Kv1.2 channels suppress synaptic terminal hyperexcitability following action potential invasion. J. Physiol. 550, 27–33 (2003).
Southan, A. P. & Robertson, B. Electrophysiological characterization of voltage-gated K(+) currents in cerebellar basket and purkinje cells: Kv1 and Kv3 channel subfamilies are present in basket cell nerve terminals. J. Neurosci. 20, 114–122 (2000).
Alle, H., Kubota, H. & Geiger, J. R. Sparse but highly efficient Kv3 outpace BKCa channels in action potential repolarization at hippocampal mossy fiber boutons. J. Neurosci. 31, 8001–8012 (2011).
Geiger, J. R. & Jonas, P. Dynamic control of presynaptic Ca2+ inflow by fast-inactivating K+ channels in hippocampal mossy fiber boutons. Neuron 28, 927–939 (2000).
Kawaguchi, S. Y. & Sakaba, T. Control of inhibitory synaptic outputs by low excitability of axon terminals revealed by direct recording. Neuron 85, 1273–1288 (2015).
Yang, Y. M. et al. Enhancing the fidelity of neurotransmission by activity-dependent facilitation of presynaptic potassium currents. Nat. Commun. 5, 4564 (2014).
Lai, H. C. & Jan, L. Y. The distribution and targeting of neuronal voltage-gated ion channels. Nat. Rev. 7, 548–562 (2006).
Brown, D. A. & Passmore, G. M. Neural KCNQ (Kv7) channels. Br. J. Pharmacol. 156, 1185–1195 (2009).
Jentsch, T. J. Neuronal KCNQ potassium channels: physiology and role in disease. Nat. Rev. Neuerosci 1, 21–30 (2000).
Debanne, D., Campanac, E., Bialowas, A., Carlier, E. & Alcaraz, G. Axon physiology. Physiol. Rev. 91, 555–602 (2011).
Kole, M. H. & Stuart, G. J. Signal processing in the axon initial segment. Neuron 73, 235–247 (2012).
Devaux, J. J., Kleopa, K. A., Cooper, E. C. & Scherer, S. S. KCNQ2 is a nodal K+ channel. J. Neurosci. 24, 1236–1244 (2004).
Pan, Z. et al. A common ankyrin-G-based mechanism retains KCNQ and NaV channels at electrically active domains of the axon. J. Neurosci. 26, 2599–2613 (2006).
Huang, H. & Trussell, L. O. KCNQ5 channels control resting properties and release probability of a synapse. Nat. Neurosci. 14, 840–847 (2011).
Fidzinski, P. et al. KCNQ5 K+ channels control hippocampal synaptic inhibition and fast network oscillations. Nat. Commun. 6, 6254 (2015).
Cooper, E. C., Harrington, E., Jan, Y. N. & Jan, L. Y. M channel KCNQ2 subunits are localized to key sites for control of neuronal network oscillations and synchronization in mouse brain. J. Neurosci. 21, 9529–9540 (2001).
Geiger, J., Weber, Y. G., Landwehrmeyer, B., Sommer, C. & Lerche, H. Immunohistochemical analysis of KCNQ3 potassium channels in mouse brain. Neurosci. Lett. 400, 101–104 (2006).
Weber, Y. G. et al. Immunohistochemical analysis of KCNQ2 potassium channels in adult and developing mouse brain. Brain Res. 1077, 1–6 (2006).
Acsady, L. & Kali, S. Models, structure, function: the transformation of cortical signals in the dentate gyrus. Prog. Brain Res. 163, 577–599 (2007).
Henze, D. A., Urban, N. N. & Barrionuevo, G. The multifarious hippocampal mossy fiber pathway: a review. Neuroscience 98, 407–427 (2000).
Jaffe, D. B. & Gutierrez, R. Mossy fiber synaptic transmission: communication from the dentate gyrus to area CA3. Prog. Brain Res. 163, 109–132 (2007).
Pelkey, K. A. & McBain, C. J. Target-cell-dependent plasticity within the mossy fibre-CA3 circuit reveals compartmentalized regulation of presynaptic function at divergent release sites. J. Physiol. 586, 1495–1502 (2008).
Rebola, N., Carta, M. & Mulle, C. Operation and plasticity of hippocampal CA3 circuits: implications for memory encoding. Nat. Rev. Neurosci. 18, 208–220 (2017).
Leao, R. N., Tan, H. M. & Fisahn, A. Kv7/KCNQ channels control action potential phasing of pyramidal neurons during hippocampal gamma oscillations in vitro. J. Neurosci. 29, 13353–13364 (2009).
Peters, H. C., Hu, H., Pongs, O., Storm, J. F. & Isbrandt, D. Conditional transgenic suppression of M channels in mouse brain reveals functions in neuronal excitability, resonance and behavior. Nat. Neurosci. 8, 51–60 (2005).
Singh, N. A. et al. Mouse models of human KCNQ2 and KCNQ3 mutations for benign familial neonatal convulsions show seizures and neuronal plasticity without synaptic reorganization. J. Physiol. 586, 3405–3423 (2008).
Delvendahl, I., Weyhersmuller, A., Ritzau-Jost, A. & Hallermann, S. Hippocampal and cerebellar mossy fibre boutons - same name, different function. J. Physiol. 591, 3179–3188 (2013).
Brown, D. A. & Adams, P. R. Muscarinic suppression of a novel voltage-sensitive K+ current in a vertebrate neurone. Nature 283, 673–676 (1980).
Shah, M. M., Migliore, M., Valencia, I., Cooper, E. C. & Brown, D. A. Functional significance of axonal Kv7 channels in hippocampal pyramidal neurons. Proc. Natl. Acad. Sci. USA 105, 7869–7874 (2008).
Martinello, K. et al. Cholinergic afferent stimulation induces axonal function plasticity in adult hippocampal granule cells. Neuron 85, 346–363 (2015).
Wang, H. S. et al. KCNQ2 and KCNQ3 potassium channel subunits: molecular correlates of the M-channel. Science 282, 1890–1893 (1998).
Young, C. C. et al. Upregulation of inward rectifier K+ (Kir2) channels in dentate gyrus granule cells in temporal lobe epilepsy. J. Physiol. 587, 4213–4233 (2009).
Gonzalez, J. C., Epps, S. A., Markwardt, S. J., Wadiche, J. I. & Overstreet-Wadiche, L. Constitutive and synaptic activation of GIRK channels differentiates mature and newborn dentate granule cells. J. Neurosci. 38, 6513–6526 (2018).
Engel, D. & Jonas, P. Presynaptic action potential amplification by voltage-gated Na+ channels in hippocampal mossy fiber boutons. Neuron 45, 405–417 (2005).
Vyleta, N. P. & Jonas, P. Loose coupling between Ca2+ channels and release sensors at a plastic hippocampal synapse. Science 343, 665–670 (2014).
Mateos-Aparicio, P., Murphy, R. & Storm, J. F. Complementary functions of SK and Kv7/M potassium channels in excitability control and synaptic integration in rat hippocampal dentate granule cells. J. Physiol. 592, 669–693 (2014).
Jackson, M. B. & Redman, S. J. Calcium dynamics, buffering, and buffer saturation in the boutons of dentate granule-cell axons in the hilus. J. Neurosci. 23, 1612–1621 (2003).
Scott, R. & Rusakov, D. A. Main determinants of presynaptic Ca2+ dynamics at individual mossy fiber-CA3 pyramidal cell synapses. J. Neurosci. 26, 7071–7081 (2006).
Chamberland, S., Timofeeva, Y., Evstratova, A., Volynski, K. & Toth, K. Action potential counting at giant mossy fiber terminals gates information transfer in the hippocampus. Proc. Natl. Acad. Sci. USA 115, 7434–7439 (2018).
Bischofberger, J., Geiger, J. R. & Jonas, P. Timing and efficacy of Ca2+ channel activation in hippocampal mossy fiber boutons. J. Neurosci. 22, 10593–10602 (2002).
Li, L., Bischofberger, J. & Jonas, P. Differential gating and recruitment of P/Q-, N-, and R-type Ca2+ channels in hippocampal mossy fiber boutons. J. Neurosci. 27, 13420–13429 (2007).
Holderith, N. et al. Release probability of hippocampal glutamatergic terminals scales with the size of the active zone. Nat. Neurosci. 15, 988–997 (2012).
Castillo, P. E., Weisskopf, M. G. & Nicoll, R. A. The role of Ca2+ channels in hippocampal mossy fiber synaptic transmission and long-term potentiation. Neuron 12, 261–269 (1994).
Jones, R. T., Faas, G. C. & Mody, I. Intracellular bicarbonate regulates action potential generation via KCNQ channel modulation. J. Neurosci. 34, 4409–4417 (2014).
Pernia-Andrade, A. J. & Jonas, P. Theta-gamma-modulated synaptic currents in hippocampal granule cells in vivo define a mechanism for network oscillations. Neuron 81, 140–152 (2014).
Vyleta, N. P., Borges-Merjane, C. & Jonas, P. Plasticity-dependent, full detonation at hippocampal mossy fiber-CA3 pyramidal neuron synapses. Elife 5, pii: e17977 (2016).
Henze, D. A., Wittner, L. & Buzsaki, G. Single granule cells reliably discharge targets in the hippocampal CA3 network in vivo. Nat. Neurosci. 5, 790–795 (2002).
Battefeld, A., Tran, B. T., Gavrilis, J., Cooper, E. C. & Kole, M. H. Heteromeric Kv7.2/7.3 channels differentially regulate action potential initiation and conduction in neocortical myelinated axons. J. Neurosci. 34, 3719–3732 (2014).
Schwarz, J. R. et al. KCNQ channels mediate IKs, a slow K+ current regulating excitability in the rat node of Ranvier. J. Physiol. 573, 17–34 (2006).
Stegen, M. et al. Adaptive intrinsic plasticity in human dentate gyrus granule cells during temporal lobe epilepsy. Cereb. Cortex 22, 2087–2101 (2012).
Greene, D. L., Kang, S. & Hoshi, N. XE991 and linopirdine are state-dependent inhibitors for Kv7/KCNQ channels that favor activated single subunits. J. Pharmacol Exp Ther 362, 177–185 (2017).
Yue, C. & Yaari, Y. Axo-somatic and apical dendritic Kv7/M channels differentially regulate the intrinsic excitability of adult rat CA1 pyramidal cells. J. Neurophysiol. 95, 3480–3495 (2006).
Hu, W. & Bean, B. P. Differential control of axonal and somatic resting potential by voltage-dependent conductances in cortical layer 5 pyramidal neurons. Neuron 97, 1315–1326 e1313 (2018).
Elmedyb, P. et al. Modulation of ERG channels by XE991. Basic Clin. Pharmacol. Toxicol. 100, 316–322 (2007).
Yue, C., Remy, S., Su, H., Beck, H. & Yaari, Y. Proximal persistent Na+ channels drive spike afterdepolarizations and associated bursting in adult CA1 pyramidal cells. J. Neurosci. 25, 9704–9720 (2005).
Vervaeke, K., Gu, N., Agdestein, C., Hu, H. & Storm, J. F. Kv7/KCNQ/M-channels in rat glutamatergic hippocampal axons and their role in regulation of excitability and transmitter release. J. Physiol. 576, 235–256 (2006).
Brown, J. T. & Randall, A. D. Activity-dependent depression of the spike after-depolarization generates long-lasting intrinsic plasticity in hippocampal CA3 pyramidal neurons. J. Physiol. 587, 1265–1281 (2009).
Metz, A. E., Jarsky, T., Martina, M. & Spruston, N. R-type calcium channels contribute to afterdepolarization and bursting in hippocampal CA1 pyramidal neurons. J. Neurosci. 25, 5763–5773 (2005).
Yan, H. D., Villalobos, C. & Andrade, R. TRPC channels mediate a muscarinic receptor-induced afterdepolarization in cerebral cortex. J. Neurosci. 29, 10038–10046 (2009).
Tai, C., Hines, D. J., Choi, H. B. & MacVicar, B. A. Plasma membrane insertion of TRPC5 channels contributes to the cholinergic plateau potential in hippocampal CA1 pyramidal neurons. Hippocampus 21, 958–967 (2011).
Park, J. Y. & Spruston, N. Synergistic actions of metabotropic acetylcholine and glutamate receptors on the excitability of hippocampal CA1 pyramidal neurons. J. Neurosci. 32, 6081–6091 (2012).
Nagy, G. A. et al. DAG-sensitive and Ca2+ permeable TRPC6 channels are expressed in dentate granule cells and interneurons in the hippocampal formation. Hippocampus 23, 221–232 (2013).
Alle, H. & Geiger, J. R. Combined analog and action potential coding in hippocampal mossy fibers. Science 311, 1290–1293 (2006).
Chicurel, M. E. & Harris, K. M. Three-dimensional analysis of the structure and composition of CA3 branched dendritic spines and their synaptic relationships with mossy fiber boutons in the rat hippocampus. J. Comp. Neurol. 325, 169–182 (1992).
Rollenhagen, A. et al. Structural determinants of transmission at large hippocampal mossy fiber synapses. J. Neurosci. 27, 10434–10444 (2007).
Bischofberger, J., Engel, D., Li, L., Geiger, J. R. & Jonas, P. Patch-clamp recording from mossy fiber terminals in hippocampal slices. Nat. Protoc. 1, 2075–2081 (2006).
Huang, Z. et al. TRIP8b-independent trafficking and plasticity of adult cortical presynaptic HCN1 channels. J. Neurosci. 32, 14835–14848 (2012).
Hines, M. L. & Carnevale, N. T. The NEURON simulation environment. Neural Comput. 9, 1179–1209 (1997).
This work was supported by the BBSRC (BB/L000679/1; M.M.S), Wellcome Trust (WT087363MA, M.M.S., D.A.B.), and the EU H2020 program (grant agreement No. 785907, HBP SGA2, M.M, EG). We thank Dr. Liyi Li (University of Freiburg, Germany; current address: University of Basel, Switzerland) for invaluable help and advice on mossy fiber bouton patching.
The authors declare no competing interests.
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Martinello, K., Giacalone, E., Migliore, M. et al. The subthreshold-active KV7 current regulates neurotransmission by limiting spike-induced Ca2+ influx in hippocampal mossy fiber synaptic terminals. Commun Biol 2, 145 (2019). https://doi.org/10.1038/s42003-019-0408-4
Frontiers in Physiology (2020)