Chimpanzees are traditionally described as ripe fruit specialists with large incisors but relatively small postcanine teeth, adhering to a somewhat narrow dietary niche. Field observations and isotopic analyses suggest that environmental conditions greatly affect habitat resource utilisation by chimpanzee populations. Here we combine measures of dietary mechanics with stable isotope signatures from eastern chimpanzees living in tropical forest (Ngogo, Uganda) and savannah woodland (Issa Valley, Tanzania). We show that foods at Issa can present a considerable mechanical challenge, most saliently in the external tissues of savannah woodland plants compared to their tropical forest equivalents. This pattern is concurrent with different isotopic signatures between sites. These findings demonstrate that chimpanzee foods in some habitats are mechanically more demanding than previously thought, elucidating the broader evolutionary constraints acting on chimpanzee dental morphology. Similarly, these data can help clarify the dietary mechanical landscape of extinct hominins often overlooked by broad C3/C4 isotopic categories.
Diet is integral to understanding the behaviours and adaptations of extant and extinct primate species alike. Nowhere is this more salient than in the evolution of the hominin tribe and the emergence of modern day humans, as the majority of dietary inferences must be constructed from a patchwork of fossilised craniodental remains. Food mechanics are likely a substantial driver in the adaptation of the dental complex and the constraints that these place on the efficiency of food processing. Understanding how the form of teeth relates to their function therefore requires a synthesis of knowledge over both tooth structure and the mechanical properties of the critical foods that resist being broken down1.
In chimpanzees (Pan troglodytes), direct behavioural observation and indirect methods such as isotopic and faecal analysis have allowed a rather in-depth knowledge of what their diets are composed of2,3,4,5,6,7,8,9,10, and thus allow for some comparison with the putative diets of the earliest hominins11. However, in such studies, foods are still largely categorised in very broad terms (e.g., fruits, leaves, bark) that do not faithfully track their mechanical properties12. In addition, accessing foods often includes the removal of external tissues with the teeth to access the nutrients within. The mechanical properties of such tissues can vary substantially and can instigate distinct oral feeding practices. Such processing is termed ingestion, which is often facilitated by the anterior dentition and is distinct from mastication, where food is cyclically processed by posterior dentition before being swallowed13. The mismatch between the mechanical characteristics of foods and how they are processed orally often makes it difficult to understand the physical conditions that foods exert on teeth and can lead to an oversimplification of this vital interface. Therefore, comparative studies of ingestive behaviours and food mechanical properties in large bodied apes, like chimpanzees, are essential to fully understand relationships between craniodental form and function in fossil hominins.
Chimpanzees allow for an interesting comparison of feeding in two evolutionarily relevant hominin habitats. The tropical forest is analogous to the original stem hominin habitat14, whilst in comparison the savannah woodland mirrors the ecological conditions that drove later hominin adaptation and the emergence of Homo15 (Fig. 1). Currently our understanding of chimpanzee dentition and its functional aspects are limited by a lack of data on the broader dietary mechanical challenges faced species-wide16,17. In fact, data on the mechanical properties effectively hail from one tropical forest18, and it is doubtful these values accurately reflect the dietary variance of the species. Unlike forest-dwelling chimpanzees, savannah chimpanzees tend to incorporate and rely upon many non-fruit items19. Isotopic studies conducted on chimpanzee populations have established the species firmly in the C3 feeding category, meaning that in all habitats chimpanzees primarily feed on tree products that utilise a C3 photosynthetic pathway20,21,22,23. Continued isotopic research has indicated that across chimpanzee habitats, from rainforest to savannah, the values of δ13C and δ15N vary significantly21,22. These patterns are thought to occur because savannah chimpanzees rely more on plant foods produced under drier environments with reduced canopy cover compared to those of their forest counterparts. However, it remains unclear if utilising foods from different environments affects food material properties in different chimpanzee populations and how this is related to isotopic signatures.
Such a relationship could prove invaluable for reconstructing the diets of extinct hominins. Early hominins, with the exception of Homo, show increasing craniodental robusticity over time11,24. Such morphological change is thought to represent, at least in part, adaptation to more mechanically challenging foods25,26,27,28,29. This seemingly correlates well with a broadening of hominin diets over evolutionary time, as demonstrated by the incorporation of a greater percentage of C4 resources11. However, the instigation of this adaptive morphology predates the incorporation of large amounts of C4 resources into the hominin diet11,24,30,31,32. This may indicate that the dietary mechanical pressures that predisposed early hominins to increased craniodental robusticity are in fact to be found in C3 as well as C4 food resources of the mosaic woodland environment.
To investigate the mechanical variance in chimpanzee diets, we measured the mechanical properties of commonly consumed plant foods of two communities inhabiting rather disparate environments. This dataset was paired with carbon and nitrogen stable isotope data from plants and hair to determine whether isotopic differences were related to mechanical variance. We hypothesised that even accounting for plant baseline, isotopic signatures will be distinct between the two chimpanzee populations and the utilisation of different biomes will promote the oral processing of more mechanically challenging foods by the savannah chimpanzees of Issa, Tanzania, compared to the rainforest population of Ngogo, Uganda.
Stable isotope data
We found that with a mean of 3.0‰, the δ15N plant values at Issa are lower than what is commonly found in chimpanzee habitats. For Ngogo plants, Carlson33 reported a mean of 4.5‰ (n = 246). A comparison between the δ15N values of the two plant datasets controlling for sample type (fruit or leaves) and plant species revealed these differences in δ15N are significant between Issa and Ngogo plant foods (χ2 = 7.36, df = 1, p = 0.006) (Fig. 2a, b). However, the same comparison between δ13C plant values from Issa and Ngogo33 revealed that on the broad scale the sites were indistinguishable in carbon (χ2 = 0.13, df = 1, p = 0.714) (Fig. 2a, b). Samples of the sedge family Cyperaceae from Ngogo had a high mean δ13C value of −11.6 ‰, whereas the single grass sample we measured from Issa had a more typical C4 plant value of −15‰ (Table 1).
We obtained novel δ13C and δ15N values for a total of 51 hair sections (obtained from 11 individuals) from the Issa chimpanzees and 85 hair sections (obtained from 13 individuals) for Ngogo. Means and standard deviation as well as fractionation factors between mean isotope values of plants and chimpanzee hair isotope values (Δplant–hair) are shown in Table 1.
Both data sets covered the different seasons of the year in an attempt to deliver an annual isotopic spectrum of adult chimpanzees at both sites. Average temporal isotopic variation within individual hair samples is moderate at Ngogo (0.32‰ in δ13C, 0.40‰ in δ15N) and also at the savannah site of Issa (0.38‰ in δ13C, 0.46‰ in δ15N); this difference in variation between sites is much smaller than the analytical error and thus not biologically meaningful. This conformity between sites was not expected given the substantial differences in annual rainfall patterns; as one would assume more striking effects of seasonality in the Issa population than in Ngogo. Our model results (see Methods for details) show that the differences between chimpanzees from Ngogo and Issa were highly significant in the δ13C values (χ2 = 61.45, df = 1, p < 0.0001) and the δ15N values (χ2 = 80.67, df = 1, p < 0.0001), with Issa chimpanzees being less depleted in 13C, and much lower in 15N (Fig. 2c, Table 1).
At both sites combined, we made 829 (Ngogo n = 488 and Issa n = 341) measurements of toughness (R) and 557 (Ngogo n = 321 and Issa n = 236) measurements of elastic modulus (E) on foods that were orally processed. These measurements included 17 plant species from Ngogo (Table 2) that comprised all species observed above 1% of the feeding time of chimpanzees during 36 h of dry season focal feeding observations. These species feeding times agreed well with long-term observations of the dry season at this site8. At Issa, 19 species were tested, including samples from Ficus, Saba, and Garcinia (Table 3) that are considered year-round staple foods10. In the dry season at Issa, chimpanzees are thought to rely more on the woodland plant genera, and our sample reflected this with the inclusion of eight mainly woodland species.
Values for R and E of orally processed foods overlapped between the two sites. However, there was a noticeable difference, particularly in the range of the values. At Ngogo, toughness ranged from 15 to 7694 J m−2, with 0.014–82 MPa for the elastic modulus, but at Issa, both toughness and elastic modulus could be much higher: 6.7–28,869.2 J m−2 toughness and 0.013–799 MPa for the elastic modulus. The data were then broken down into food tissue categories (Fig. 3) to help elucidate what may be driving the differences in food mechanics between sites. Values within comparable categories had similar ranges in each location that fell within the values previously published for primate food mechanical properties18,34,35. The higher toughness values at Issa were significant for fruit exocarp (Mann–Whitney U test, W = 2633.5, p < 0.001). Lower values recorded for mesocarp at Issa were also significantly so (W = 9934, p < 0.001). Similarly, values in leaf laminar tissues were significantly lower at Issa (W = 2265, p = 0.007), yet there was no significant difference for the toughness of leaf midrib (W = 2267, p = 0.1703). There were also differences in the recorded E of the comparable food tissues. The exocarps of fruits from Issa were significantly stiffer than those from Ngogo (W = 442.5, p < 0.001), whilst the fruit mesocarp from Issa was of a significantly lower stiffness than those of Ngogo (W = 97705, p < 0.001). A similar relationship was observed for leaf laminar tissue (W = 1157, p = 0.005). Recorded values of both R and E demonstrate the most extreme disparity in the exterior casings of fruits that must be breached to obtain nutrient rich mesocarp (see video S1). In Issa exterior tissues such as fruit exocarp demonstrate considerably higher values than are seen in other plant tissues.
Figure 4 provides a more in depth exploration of the external food casings. The Ngogo study area is mostly covered by moist evergreen and semi-deciduous forest from which all the foods in this study were sampled; therefore, all Ngogo exocarp data were pooled and labelled as forest species. However, the external casings from Issa, a mosaic habitat with multiple biomes, have been broken down into fruits from the gallery forest or fruits from the savannah woodland species. Here it is clear that the largest differences in both R and E were found in savannah woodland fruits. There was a significant difference between the three categories (forest fruits, gallery forest fruits and savannah woodland fruits R, Kruskal Wallis test: χ2 = 79.3, p < 0.001 and E, χ2 = 78.8, p < 0.001). A Dunn’s test of multiple comparisons showed that all categories were significantly different from each other in both toughness and stiffness.
Mechanical data from foods consumed by P. troglodytes schweinfurthii in Ngogo conformed well to those of Vogel et al.18 measured from chimpanzee populations at Kanyawara. All Ngogo data for toughness and stiffness remained at relatively low levels (Fig. 3a, b). Similarities between Kanyawara and Ngogo are not surprising, as both sites are within the Kibale National Park, with foods comprising of comparable plant species. Indeed, there is up to 73% overlap in feeding species between the two sites3,7. Ngogo and Kanyawara provide an example of tropical rainforest, and the chimpanzees at both sites have diets comprised largely of fleshy ripe fruits even in times of reduced production3,8. This pattern of high fruit consumption characterises chimpanzees inhabiting tropical and subtropical moist broadleaf forests21. In such biomes it is likely that lower seasonality and higher fruit availability compared to savannah woodland sites means that the majority of oral processing reduces the mechanical challenges to teeth. At Issa, the mesocarp of fruit, leaf laminar tissue, and leaf midrib also manifested within this rather narrow range (Fig. 3a, b). These are all tissues that are likely masticated by molars and then subsequently swallowed. Previous hypotheses surrounding the molar morphology of Pan have suggested that gracile molars with thin enamel are a derived trait adapted for comminuting large amounts of easy to process foods, along with some (possibly seasonal) fracture resistant foods, such as foliage18. The loss of thick enamel is likely due to a relaxation of selection pressures that necessitated strong durable crowns adapted to either hard and/or abrasive food tissues. Our data go some way to supporting this hypothesis, demonstrating a lack of variance in the mechanical properties of tissues likely masticated by chimpanzees across our study sites.
Despite the overlap in masticated tissues, data from Issa presents a divergence from this mechanical dietary uniformity. Substantial differences occur in both the toughness (Fig. 4a) and elastic modulus (Fig. 4b) in the external casing of savannah fruits at Issa. Such mechanically challenging tissues will necessitate ingestive processing to access consumable tissues (see for example Supplementary Movie 1). It is noteworthy that the mean values for these tissues, R = 1794.0 (sd 5435.2) J m−2 and E = 50.1 (sd 91.3) MPa, exceed those of Bornean orangutans (R = 1152.9 J m−2 and E of 3.08 MPa, respectively), which are generally considered to consume the most mechanically challenging diet of all the great apes36. At Issa, the highest values were generated primarily by the woody valves of Julbernardia sp., which are comparable values to other woody legume pods eaten by primates1,37 and the resilient outer exocarp of Strychnos sp. (Table 3). The exocarp of other savannah species within our data set also demonstrated generally higher toughness and stiffness estimates when compared to foods from rainforest, gallery forest, and values obtained from the literature1,18,36 (Fig. 3a, b). We know very little about the mechanical properties of savannah plants, but these are likely to demonstrate a greater variability, as such plant species must have different adaptations to water stress events. All this would indicate that when feeding is more concentrated in the savannah woodland habitats, as is the case at Issa during the dry season10, chimpanzees face external plant tissues that are substantially more demanding than those encountered by their conspecifics within Kibale National Park.
Whilst extractive and percussive foraging behaviours are reported in savannah chimpanzee populations38,39,40,41, the majority of plant foods are likely processed orally. In chimpanzees, oral ingestive processing is done primarily by the anterior teeth2,38,42 (an example of such action can be found in Supplementary Movie 1). These feeding behaviours coincide well with morphology, as high forces and unique loading regimes are likely to be necessary in the husking of more mechanically challenging foods, such as those demonstrated at Issa. Unlike basal Miocene apes and later members of the human ancestral clade, chimpanzees have quite derived anterior teeth, most obviously in the size and morphology of the incisors43. Chimpanzee incisors are characterised by their large broad spatulate shape16,42,43,44, presenting a more procumbent posture and sharp cutting edge, maintained through a thinning of the lingual enamel, which may also reduce stress on the crown during ingestion45. Uniquely amongst hominoids, the lower incisors have converged to the morphology of the uppers43. Both upper and lower incisors therefore offer a large optimally angled cutting tool, well adapted for initiating and propagating fracture in foods46. Furthermore, each anterior tooth is anchored by a markedly long and large tooth root47, making them well equipped to deal with high forces that are likely inflicted on these teeth during the ingestive processing of mechanically challenging foods. It therefore appears that the anterior teeth of chimpanzees form the workhorse of the chimpanzee dentition, and are well adapted to deal with mechanical challenges arising from foods. These teeth are likely utilised to overcome the higher mechanical challenge presented by the external casings of savannah plants within the Issa environment. Such external barriers must be breached in order to gain access to internal nutrient tissues.
Isotopic signatures measured from chimpanzee hair samples show a significant difference between sites in δ15N and δ13C values (δ13C values: χ2 = 61.45, df = 1, p < 0.0001 and the δ15N values χ2 = 80.67, df = 1, p < 0.0001). These differences become apparent in the substantial differences in Δplant–hair isotope values, which show the behavioural difference in chimpanzee habitat utilisation when controlling for isotopic baseline effects in potential plant foods. For δ15N, this discrepancy can be explained by the significant differences in the plant isotope baselines between sites (χ2 = 7.36, df = 1, p = 0.006), which are probably driven by the isotope values of non-fruit items such as leaves (see Table 1). This indicates that previous attempts20,21 to explain the relatively low δ15N values in the Issa chimpanzees in the absence of plant baseline data require revision. Low δ15N values in the Issa chimpanzees are best explained by generally depleted plant baseline values in this woodland mosaic habitat, and not necessarily by the chimpanzees’ heavy consumption of nodulating (soil nitrogen fixating) plants. Moreover, an overall depleted δ15N signature seems to be more common in savannah chimpanzee sites than previously assumed, as this low δ15N pattern has also been observed at several other savannah chimpanzee sites across Africa, including Kayan in Senegal22, as well as in several unpublished datasets from West Africa (Oelze personal communication).
In this study, however, we focussed on the site specific signatures in δ13C, as they are highly relevant for understanding paleodiets in the fossil record. Measurements of δ13C can be obtained from ancient dental enamel, whereas the analysis of δ15N is limited to well-preserved organic material containing substantial amounts of nitrogen. The plant δ13C values in our study indicate that on a general scale, the isotopic variance between the two habitats is minimal. However, chimpanzee hair isotope values significantly differ in δ13C. This evinces to two main outcomes. Firstly, chimpanzees do not always simply resemble the isotopic characteristic of the environment they inhabit, but they have feeding preferences and select microhabitats suitable to meet their dietary demands. Our δ13C data suggest that Issa chimpanzees do not feed solely on plant foods (mainly ripe fruits and smaller quantities of leaves) derived from dense gallery forest patches, but rely on 13C enriched plants in the open areas of the woodland savannah, which is concurrent with observational and faecal analysis at Issa4,10. This is in line with isotopic evidence reported from chimpanzees and their plant foods at the savannah site of Kayan in Senegal22 and with what can be assumed from work at other savannah sites like Fongoli, although respective δ13C plant data are not yet available23. Secondly, δ13C values from hair samples differed between sites, but this variance does not resemble the vast differences reported between C4 (savannah) and C3 (forest) dependent fossil hominin species in East Africa11,48, primarily because no known population of chimpanzees has been found to habitually consume C4 plant foods23. Yet it appears these smaller scale differences may have rather large implications in the acquisition of food and the mechanical challenges encountered in contrasting biomes. Such subtle differences could therefore be of interest to paleoanthropologists reconstructing diets of the past.
A somewhat restrictive diet dominated by C3 plants—as found in chimpanzees11,49—is often assumed to be somewhat mechanically narrow, i.e., associated with easy to process fruits and forest products. Our data indicate that this is not always the case. Plant tissues consumed by chimpanzees that utilise a C3 photosynthetic pathway can demonstrate pronounced mechanical variance and challenges. Broad and easily observable isotopic categorisations based on photosynthetic pathways are critical to our understanding of paleo-environments, but alone these proxies may offer little indication of the finer scale mechanical behaviour of plant foods; it is this which is likely to be driving the adaptations of the craniodental complex of African Plio-Pleistocene fossil hominins.
Although discussion is ongoing concerning the exact paleoenvironment that the australopiths of Pliocene East Africa inhabited, there is some consensus that this niche was either wooded shrubland or wooded grassland, similar to the mosaic savannah woodland environment of extant savannah chimpanzees15,50. Fossil findings have also indicated that members of Pan have long used these habitat types in sympatry with early Homo, a relationship with the human lineage that may have endured since the divergence of Pan and hominins51. Middle Pliocene australopiths such as Ardipithicus ramidus and Australopithecus anamensis possess remarkably comparable isotopic signatures with savannah chimpanzees, suggesting they relied on a C3 dominated diet49,52,53. Whilst perhaps savannah chimpanzees are an imperfect morphological analogy for these early hominins, there are some dental and gnathic similarities (e.g. increased procumbancy and larger incisors) that appear somewhat reduced in later occurring Pliocene hominins (such as Au. afarensis) and even more so in Pleistocene hominins (such as Homo and Paranthropus)32,54,55. The coupling of our mechanical and isotopic data suggests that savannah dwelling members of Pan that utilise similar habitats and eat mechanically similar foods to our earliest relatives could provide a reasonable extant analogue for exploring early hominin feeding ecology. Further to this, our results indicate that there may have been a shift towards more mechanically challenging foods associated with the hominin transition to exploiting more wooded environments that likely predates the general hominin trend for increased C4 consumption.
Our quantitative results of food mechanical properties indicate that many plant tissues masticated by chimpanzees do inhabit a rather narrow dietary range and could be considered rather easy to process. However, this does not comprehensively represent the extent of chimpanzee diets, as harder to process plant tissues can represent substantial contributions to the diets of some populations. We do not advocate that mechanically challenging food items in the chimpanzee diet are only found in savannah environments or that chimpanzees routinely process such foods at all savannah sites. Indeed, different chimpanzee populations have been shown to use seemingly similar environments quite differently with regard to foraging habits20. Rather, we show that the possibility exists that in the resource limited savannah woodland environment, chimpanzees choose different foods, some of which are more mechanically challenging than has been considered the dietary norms for this species36. Importantly, these tissues are produced by C3 plants, indicating that both C3 and C4 plants can manifest as mechanically challenging plant tissues and both may be responsible for driving dental adaptation. Mechanically challenging tissues, like the external casings of savannah plants, are probably processed to a large extent with the anterior dentition. These teeth are likely to incur larger and more variable forces than the postcanine teeth, as internal tissues that are masticated present only a limited mechanical challenge. Understanding if there is a functional driver behind morphological features of the teeth of chimpanzees and indeed fossil hominins will require a further expansion of the current knowledge of both food mechanical properties and ingestive behaviours on a pan-African scale to reduce our reliance of mechanical property data from singular sites.
Two sites chosen for this study were the Ngogo Chimpanzee Project and the Greater Mahale Ecosystem Research and Conservation Project (GMERC, formerly Ugalla Primate Project). Both sites were investigated during the dry season, which in both vicinities is associated with a decrease in fruit production and arguably presents a period of greater dietary stress for the chimpanzee communities4,7. Chimpanzee hair samples for isotope analysis were collected opportunistically during a 12+ month study period at Ngogo (2012–2013) and Issa (2013–2014) within the framework of the Pan African Programme (http://panafrican.eva.mpg.de/). They represent the annual spectrum of isotope values at each site. At both sites, the samples represent plants from both wet and dry seasons (as defined below).
Ngogo—The Ngogo study area is situated centrally in the Kibale National Park in south-western Uganda7,56. The park consists of an area of 795 km2, dominated by moist evergreen, with some seasonally deciduous, forest. Tree species are a transition between montane and lowland forest7,56 (Fig. 1a). The area receives high rainfall with the yearly average ranging from 1400 to 1600 mm. This is fairly evenly distributed throughout the year, but dry seasons can be defined as two low rainfall levels between June–July and December–February7,56. The study area is home to a chimpanzee population of close to 200 individuals that have been continuously observed since 1995. The chimpanzees are well-habituated allowing direct observation of food selection and feeding behaviours7.
Issa—The GMERC is located in the Issa valley that lies 100 km east of Lake Tanganyika. The site is a mosaic habitat dominated by savannah woodland (Brachystegia and Julbernardia) but punctuated by evergreen gallery forests, swamps and grassland (Fig. 1b). Seasonality is high at Issa with two discrete seasons: a wet (October–April) and dry (May–September). The annual rainfall is lower than at Ngogo, averaging 1220 mm per annum with levels dropping to <100 mm in the months of the dry season10,57,58. Research on chimpanzees was first conducted in this region in 2001–20034, with a permanent research presence initiated in 2008 by the GMERC that has since been maintained. The Issa community is considered semi-habituated; current research is focused on a 85 km2 study area where genetic analysis has identified 67 individuals57,58.
Sample collection for mechanical properties
Ngogo—As the population is well habituated at this site it was possible to make direct observations of what was consumed by individuals. This information was checked against the substantial literature on chimpanzee diet in the Ngogo study area7,8,59 to confirm that the items seen eaten were typical for the time of year and habitat. With such guidance, we determined the most important foods to test by conducting day-long follows of chimpanzees, employing the focal techniques used by Vogel et al.18. This entailed picking a focal animal from within the group and recording their behaviour continuously for 10 min. After this period elapsed, another individual was then selected and observed. This way one can garner observations across a large group of individuals18.
Knowing what is being eaten allowed the selection of foods for measurements of the mechanical properties of individual tissues either ingested or masticated by chimpanzees. Samples were obtained by two main methods. Foods were either dropped by focal animals, this may be because a plant tissue was not consumed, or it was dropped in the process of eating. However to increase the number of samples for testing, food items were also acquired directly from trees accessed using canopy access techniques60 that chimpanzees had been observed feeding in.
Issa—The semi habituated state of the population at Issa does not permit the kind of all-day follows of chimpanzees used at Ngogo. Often finding groups of individuals can take some time and the amount of time following is greatly reduced when compared to Ngogo. This means that direct observations of feeding can be reduced to a matter of minutes per day. Therefore, direct observations were used on an opportunistic basis and foods were collected following confirmation that a certain food item was eaten by the chimpanzees. However, due to the low levels of direct observations we also used information from over 4 years of dietary research conducted at Issa which has identified the major food sources from faecal sieving and direct observations alike10 this allowed us to target the most commonly consumed dry season foods. In both sites, whenever foods were selected by humans, efforts were taken to match overt cues of readiness of foods for consumption.
Mechanical properties testing
We measured two main mechanical properties that are particularly pertinent to the breakdown of food: toughness and elastic modulus. We defined toughness as the energy needed to propagate a crack through a material. An estimation of the energy needed to generate a new surface is made and then this is divided by the actual surface area of one side of the crack. The resulting value is termed R with the units of joules per metre squared (J m−2)1,61. This is integral to understanding how foods resist cracks being initiated and propagated by teeth: foods of higher toughness will be more resilient and harder to breakdown during ingestion and mastication. Toughness has been utilised as a dietary proxy in many studies of primate feeding ecology and has helped understand the interface between teeth and foods1. The elastic (Young’s) modulus (E) of a material is its resistance to reversible deformation, measured as the stress (force per unit area) that produces a strain (a proportional change in dimensions). This can be estimated from the slope of an initial linear region of a stress–strain curve and has units that are usually given in the megapascal (MPa) range for foods consumed by chimpanzees and other primates1.
Whenever possible, foods were separated into broad plant anatomical categories, such as exocarp and mesocarp for fruits, with leaves divided into laminar tissue vs. midrib/veins, concordant with Vogel et al.18,62. Samples of these tissues were tested individually. To deal with anisotropy, tests were performed in the direction relevant to feeding. This was determined from feeding remains or video evidence. If this was not possible, multiple orientations were tested. All tests in this study were performed on a portable universal testing machine designed for use in the field (Lucas Scientific FLS-1). This machine consists of a hand-cranked movable crosshead and was equipped with a force transducer to measure the resultant forces and a linear variable displacement transducer that measured accurately movements in the crosshead. The equipment is powered by and interfaces with a laptop computer upon which custom built software allows the calculations of the main material properties of foods. There are a multitude of tests available to measure mechanical properties and the tester houses a range of accessories and rigs that can be employed to measure R and E. Selection of a test depends partly on the size and shape of food items and components and on how chimpanzees process them. Below, we outline the tests that we used during this study.
Toughness: Measuring this required the generation of a fracture. We utilised the displacement-controlled action of blades for this purpose, measuring the force needed to propagate a crack through a given area of material. Use of a blade allowed a fracture to be directed through a heterogeneous specimen, such as a leaf for example, such that it accords with the types of fracture seen on samples eaten by chimpanzees. One of the major causes of error in recording toughness via this method is that the interface between blade and material will generate friction and may lead to an overestimate of toughness if not separated out from fracture. However, such friction can be estimated simply by running a second pass of the blade after a fracture has been formed. The blade needs to pass through an identical displacement, with the work recorded, being not that required to produce a new surface, but rather to overcome frictional interactions. This second pass can be subtracted from the originally recorded energy to give a more accurate figure of fracture toughness61.
Bulk food items, such as substantial pieces of fruit flesh, had their toughness estimated by employing the wedge test. A sharp wedge (circa 15°) would be driven into a food specimen of known dimensions for a known displacement, thus generating a crack within it. A second pass, as described above, compensates for the influence of friction. The energy actually used in crack formation, obtained by deducting the work done in the second pass from that in the first, was then divided by the area of the newly created surface to obtain an estimate of the toughness61. Sometimes the amount of testable material is too small to be wedged. Such tissues are sheet or rod-like structures. When these circumstances arise, a single blade, or two crossing blades as in a pair of scissors, was used to propagate a crack though a material of known dimensions. Again, a second pass is used to compensate for friction between the blade and food or between the two passing blades61,63.
Elastic Modulus: Measuring the elastic modulus of primate foods has become far easier in recent years with the onset of developments in indentation methods (for more detail, see Talebi et al.35 and van Casteren et al.64). Blunt indentation uses hemispherical indenters to measure the elastic modulus of a material quickly and with very little sample preparation. All blunt indent tests follow basic load relaxation conditions: a material is loaded slowly at a consistent rate for around 10 s and the resultant “force ramp” is recorded. After 10 s, the displacement is then held constant whilst measuring decay of the load for a further 90 s or until the load becomes constant. A curve is fitted to this relaxation behaviour allowing the calculation of an instantaneous (Ei) and infinite (E∞) elastic modulus. These terms effectively represent the upper and lower bounds of a material’s elastic resistance and the ratio of the two values (E∞/Ei) indicates the rate sensitivity of a material. Whilst neither of these values is an ideal representation of what happens in the mouth for this particular study, we consider Ei to be a more useful measure when considering ingestion and mastication and is used primarily in this investigation64.
We used two types of blunt indent test for this study. The first, a bulk indent test, used a large hemispherical probe (of 3.6 mm radius) for measuring the elastic modulus of bulk food items, like fruit flesh. A sample must be cut so that is stable and has a flat surface normal to the probe. Care must be taken that the sample is sufficiently thick (≥2 mm) and that the indent does not exceed 10% of the sample thickness to avoid influence of the substrate on which it rests64. The second test is a membrane test that can be used on sheet-like materials like leaves and, in some cases, a peel-like exocarp of a fruit. A test specimen was clamped between two transparent plates that have aligned circular holes, 2 mm in radius, in their centre. A hemispherical probe of 0.25 mm radius is then used to measure the elastic modulus of a specimen—laminar leaf tissue or some external fruit peels by pressing down on a specimen exactly in the centre of the exposed disc of tissues. In this test, the total deformation needed to be less than the total thickness of the specimen being tested to avoid error. After testing, the material was checked for visible damage to ascertain if there was damage due to cellular collapse; such test results were discarded35. Both these blunt indentation tests followed the basic load relaxation method described above.
Some foods cannot be indented because their shape and size does not allow for this, e.g., specimens in the form of rods. In these cases, we resorted to more traditional compression tests where possible. Cylinders of material of known dimension were compressed and the elastic modulus calculated as the slope of the initial region of the stress strain graph65. For woody material, or that arranged in a rod-like manner, 4-point bending tests were used to calculate the elastic modulus. This is where a beam of known dimensions is bent and the elastic modulus estimated from the elastic phase of this bending behaviour65.
Stable isotope sampling and analysis
For this study we analysed 11 hair samples from the chimpanzees at Issa, and 13 hair samples from the Ngogo chimpanzees in Kibale. Chimpanzee hair samples exported from Uganda and Tanzania were done so following the regulations set out in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). CITES Permit No. UG003042 (Uganda) and CITES Permit No. 28753 (Tanzania). Samples consist of at least 10–15 hairs each and were obtained non-invasively from fresh or recent nests (nest decay stage 1 or 2, see Kouakou et al.66) (Supplementary Table 1), which were associated to four distinct nests groups at Issa and to five nest groups in Ngogo. By focussing on nest groups we tried to ensure the sampling of different members of a chimpanzee party with the aim to minimise potential errors easily introduced by pseudoreplication67. Hair samples were prepared following the procedure outlined in detail by Oelze68, with an emphasis on removing potential infant hairs and lipid contaminants from the material used for isotope analysis. All hair used contained root bulbs in the telogen stage and was cut sequentially in 5 or 10 mm long sections as weight for analysis allowed (<3.5 mg). Each hair yielded multiple isotope measurements with hair section isotope values reflecting the previous 2 weeks (5 mm) or one month (10 mm) of diet if human hair growth rates are used as a proxy. As a result, each complete hair sample reaches several months back into time and covers on average six previous months of chimpanzee dietary behaviour68.
Plant carbon isotope data from Ngogo were available due to the extensive work of Bryce Carlson and could be extracted from the literature33. Although several peer-reviewed publications contain the carbon data from his work, we decided to refer to his PhD dissertation, as it contains both δ13C and δ15N data on Ngogo plants, reporting means for samples for which multiple samples had been collected. Ngogo plant samples were collected in the different seasons of 2009 and 2010 and represent the top 40 plant foods known to be preferred by the Ngogo chimpanzees33,69. To ease the comparison with the Issa plant data, we considered only the data obtained from fruits and leaves (n = 184, reported mean isotope values n = 34, see Supplementary Table 2), including fruits, seeds, pulp and grasses but excluding roots, bark, flowers, and piths. These plant samples were selected based on the chimpanzees’ feeding preferences and thus encompass the different levels of the canopy as exploited by the Ngogo chimpanzees, including ground and high canopy foods69,70. In Issa we collected a small selection of representative plant samples (n = 32) for stable isotope analysis in the wet and dry seasons of 2015 and 2016. We focussed on plant foods assumed to be essential for the Issa chimpanzees based on the literature10, feeding signs, and the presence of the tree species in the GMERC’s phenology inventory. Thus food plant samples were predominantly obtained from miombo woodland and gallery forest habitat types and much less so from open savannah areas. All Issa plant materials are represented by bulk fruits (exocarp, mesocarp, seeds) and leaves, but also by one sample of grass from the open savannah (Supplementary Table 3). As in Ngogo, plant sampling followed evidence of chimpanzees’ feeding selection and thus encompasses samples from the different layers of the canopy. Ripe fruit and leaves were predominantly collected after being dropped to the ground by various animals feeding in the canopy, whereas some mature leaves and terrestrial herbs such as Aframomum sp. and the unidentified grass were collected from the subcanopy level. Both datasets are slightly over representative of fruit over leaves, which we consider to resemble chimpanzee feeding preferences. Plant samples exported from Tanzania were done so with the permission of the Tanzanian Chamber of Commerce, Industry and Agriculture (Permit No. A025760) and adhered to Phytosanitary conditions for export (Phytosantary certificate No. 215903). All plant materials were thoroughly dried, homogenised to a fine powder in a pebble mill, and ~2 mg were weighed into tin capsules for isotopic measurement.
All stable isotope measurements were performed in a Flash 2000 – HAT elemental analyser (Thermo Fisher Scientific, Waltham, USA) coupled via ConFLo IV (Thermo Fisher Scientific, Waltham, USA) with a MAT 253 mass spectrometer (Thermo Fisher Scientific, Waltham, USA) at the commercial stable isotope laboratory IsoDetect in Leipzig, Germany. The stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) are expressed as the ratio of 13C/12C and 15N/14N ratios, respectively, using the delta (δ) notation in parts per thousand or permil (‰) relative to the international standard materials Vienna PeeDee Belemite (vPDB) and atmospheric N2. The analytical error calculated from repetitive measurements of international (USGS25, USGS40, and USGS41 for N; IAEA-CH6, IAEA-CH7 and IAEA-CH3 for C) and lab-internal standards (caffeine, methionine) included in each run is less than 0.2 ‰ (2σ) for δ13C and δ15N. To assure analytical quality we excluded all hair isotope data with atomic C:N ratios outside the acceptable 2.6–3.8 range71.
For statistical analysis we used R (version 3.4.1, R Development Core Team 2017. We tested the response variables δ13C and δ15N in plant samples by running two separate mixed models with Gaussian error structure containing the fixed effect of ‘site’, and the control predictor ‘plant sample’, as well as the random effect of ‘plant species’, accounting for multiple measurements per taxon in the datasets used. We excluded the C4 grass samples from both plant datasets in our analysis due to low sample size for this control variable. We calculated p-values for both models by comparing a full model against a null model excluding the fixed effect of ‘site’ with the function ANOVA. To compare the δ13C and δ15N values in chimpanzee hair between sites, we also tested each isotope value as a response in a linear model with Gaussian error structure. In both models we included the main effect of ‘site’ and the random effect of ‘hair sample’ to account for the fact that we conducted several measurements per hair sample and thus per individual. We obtained model results by running an ANOVA with the full model and a null model excluding the main effect. For all the four above models, various diagnostic plots of the residuals against fitted values confirmed normal distribution of residuals in the models. We tested variance inflation factors and found no issues with collinearity. Model stability was tested by running each model again by excluding single observations one at a time and comparing the respective model results. Stability tests showed no sign of influential cases.
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Lucas, P. W. Dental Functional Morphology: How Teeth Work (Cambridge University Press, New York, 2004).
Suzuki, A. An ecological study of chimpanzees in a savanna woodland. Primates 10, 103–148 (1969).
Wrangham, R. W., Chapman, C. A., Clark-Arcadi, A. P. & Isabirye-Basuta, G. in Great Ape Societies (eds McGrew, W. C., Marchant, L. F. & Nishida, T.) 45–57 (Cambridge University Press, Cambridge, 1996).
Hernandez-Aguilar, R. A. Ecology and Nesting Patterns of Chimpanzees (Pan Troglodytes) in Issa, Ugalla, Western Tanzania. PhD thesis, University of Southern California (2006).
Morgan, D. & Sanz, C. M. in Feeding Ecology in Apes and Other Primates (eds Hohmann, G., Robins, M. M. & Boesch, C.) (Cambridge University Press, Cambridge, 2006).
Pruetz, J. D. in Feeding Ecology in Apes and Other Primates (eds Hohmann, G., Robins, M. M. & Boesch, C.) (Cambridge University Press, Cambridge, 2006).
Watts, D. P., Potts, K. B., Lwanga, J. S. & Mitani, J. C. Diet of Chimpanzees (Pan troglodytes schweinfurthii) at Ngogo, Kibale National Park, Uganda, 1. Diet Composition and Diversity. Am. J. Primatol. 74, 114–129 (2012).
Watts, D. P., Potts, K. B., Lwanga, J. S. & Mitani, J. C. Diet of Chimpanzees (Pan troglodytes schweinfurthii) at Ngogo, Kibale National Park, Uganda, 2. Temporal Variation and Fallback Foods. Am. J. Primatol. 74, 130–144 (2012).
Lindshield, S., Danielson, B. J., Rothman, J. M. & Pruetz, J. D. Feeding in fear? How adult male western chimpanzees (Pan troglodytes verus) adjust to predation and savanna habitat pressures. Am. J. Phys. Anthropol. 163, 480–496 (2017).
Piel, A. K. et al. The diet of savanna-woodland chimpanzees (Pan troglodytes schweinfurthii) at Issa, Ugalla, western Tanzania. J. Hum. Evol. 112, 57–69 (2017).
Sponheimer, M. et al. Isotopic evidence of early hominin diets. Proc. Natl Acad. Sci. 110, 10513–10518 (2013).
Coiner-Collier, S. et al. Primate dietary ecology in the context of food mechanical properties. J. Hum. Evol. 98, 103–118 (2016).
Wright, B. W. Craniodental biomechanics and dietary toughness in the genus Cebus. J. Hum. Evol. 48, 473–492 (2005).
Potts, R. Paleoenvironmental basis of cognitive evolution in great apes. Am. J. Primatol. 62, 209–228 (2004).
Cerling, T. E. et al. Woody cover and hominin environments in the past 6 million years. Nature 476, 51–56 (2011).
Deane, A. First contact: understanding the relationship between hominoid incisor curvature and diet. J. Hum. Evol. 56, 263–274 (2009).
Klukkert, Z. S., Teaford, M. F. & Ungar, P. S. A dental topographic analysis of chimpanzees. Am. J. Phys. Anthropol. 148, 276–284 (2012).
Vogel, E. R. et al. Functional ecology and evolution of hominoid molar enamel thickness: Pan troglodytes schweinfurthii and Pongo pygmaeus wurmbii. J. Hum. Evol. 55, 60–74 (2008).
Lindshield, S. M. Multilevel Analysis of the Foraging Decisions of Western Chimpanzees (Pan troglodytes verus) and Resource Scarcity in a Savanna Environment at Fongoli, Senegal. PhD dissertation, Iowa State University (2014).
Schoeninger, M. J., Moore, J. & Sept, J. M. Subsistence strategies of two ‘savanna’ chimpanzee populations: the stable isotope evidence. Am. J. Primatol. 49, 297–314 (1999).
Schoeninger, M. J., Most, C. A., Moore, J. J. & Somerville, A. D. Environmental variables across Pan troglodytes study sites correspond with the carbon, but not the nitrogen, stable isotope ratios of chimpanzee hair. Am. J. Primatol. 78, 1055–1069 (2015).
Oelze, V. M. et al. Comparative isotope ecology of African great apes. J. Hum. Evol. 101, 1–16 (2016).
Sponheimer, M. et al. Do ‘savanna’ chimpanzees consume C4 resources? J. Hum. Evol. 51, 128–33 (2006).
White, T. D. et al. Asa Issie, Aramis and the origin of Australopithecus. Nature 440, 883–889 (2006).
Teaford, M. F. & Ungar, P. S. Diet and the evolution of the earliest human ancestors. Proc. Natl Acad. Sci. USA 97, 13506–13511 (2000).
Jolly, C. J. The seed-eaters: a new model of hominid differentiation based on a baboon analogy. Man 5, 5–26 (2009).
Strait, D. S. et al. The feeding biomechanics and dietary ecology of Australopithecus africanus. Proc. Natl Acad. Sci. USA 106, 2124–2129 (2009).
Strait, D. S. et al. Viewpoints: diet and dietary adaptations in early hominins: the hard food perspective. Am. J. Phys. Anthropol. 151, 339–355 (2013).
Lucas, P. W., Constantino, P. J., Wood, B. & Lawn, B. Dental enamel as a dietary indicator in mammals. Bioessays 30, 374–385 (2008).
Levin, N. E., Haile-Selassie, Y., Frost, S. R. & Saylor, B. Z. Dietary change among hominins and cercopithecids in Ethiopia during the early Pliocene. Proc. Natl Acad. Sci. USA 112, 12304–12309 (2015).
Ward, C. V., Leakey, M. G. & Walker, A. Morphology of Australopithecus anamensis from Kanapoi and Allia Bay, Kenya. J. Hum. Evol. 41, 255–368 (2001).
Ward, C., Leakey, M. & Walker, A. The new hominid species Australopithecus anamensis. Evol. Anthropol. 7, 197–205 (1999).
Carlson, B. Reconstructing Diet from the Ground Up: Isotopic Dietary Ecology of Chimpanzees at Ngogo, Kibale National Park, Uganda. PhD dissertation, Emory University (2011).
Constantino, P. J., Lucas, P. W., Lee, J. J.-W. & Lawn, B. R. The influence of fallback foods on great ape tooth enamel. Am. J. Phys. Anthropol. 140, 653–60 (2009).
Talebi, M. G. et al. Membrane–plate transition in leaves as an influence on dietary selectivity and tooth form. J. Hum. Evol. 98, 18–26 (2016).
Taylor, A. B., Vogel, E. R. & Dominy, N. J. Food material properties and mandibular load resistance abilities in large-bodied hominoids. J. Hum. Evol. 55, 604–616 (2008).
McGraw, W. S. et al. Feeding and oral processing behaviors of two colobine monkeys in Tai Forest, Ivory Coast. J. Hum. Evol. 98, 90–102 (2015).
McGrew, W. C. Manual laterality in anvil use: wild chimpanzees cracking Strychnos Fruits. Laterality 4, 79–87 (1999).
Pruetz, J. D. & Bertolani, P. Savanna chimpanzees, Pan troglodytes verus, hunt with tools. Curr. Biol. 17, 412–417 (2007).
Gaspersič, M. & Pruetz, J. D. Savanna chimpanzees (Pan troglodytes verus) and baobab fruits (Adansonia digitata): Investigation of percussive technology among three chimpanzee communities in southeastern Senegal. Folia Primatol. 79, 332–333 (2008).
Bogart, S. L. & Pruetz, J. D. Insectivory of savanna chimpanzees (Pan troglodytes verus) at Fongoli, Senegal. Am. J. Phys. Anthropol. 145, 11–20 (2011).
Hylander, W. L. Incisor size and diet in anthropoids with special reference to Cercopithecidae. Science 189, 1095–1098 (1975).
Pickford, M. Incisor-molar relationships in chimpanzees and other hominoids: Implications for diet and phylogeny. Primates 46, 21–32 (2005).
White, T. D., Lovejoy, C. O., Asfaw, B., Carlson, J. P. & Suwa, G. Neither chimpanzee nor human, Ardipithecus reveals the surprising ancestry of both. Proc. Natl Acad. Sci. USA 112, 4877–4884 (2015).
Kupczik, K. & Chattah, N. L. T. The adaptive significance of enamel loss in the mandibular incisors of cercopithecine primates (mammalia: Cercopithecidae): a finite element modelling study. PLoS ONE 9, e97677 (2014).
Dominy, N. J. et al. How chimpanzees integrate sensory information to select figs. J. R. Soc. Interface Focus 6, 20160001 (2016).
Kupczik, K. & Dean, M. C. Comparative observations on the tooth root morphology of Gigantopithecus blacki. J. Hum. Evol. 54, 196–204 (2008).
Ungar, P. S. & Sponheimer, M. The diets of early hominins. Science 334, 190–193 (2011).
Alemseged, Z. Stable isotopes serving as a checkpoint. Proc. Natl Acad. Sci. USA 112, 12232–12233 (2015).
White, T. D. et al. Macrovertebrate paleontology and the Pliocene habitat of Ardipithecus ramidus. Science 326, 87–93 (2009).
McBrearty, S. & Jablonski, N. G. First fossil chimpanzee. Nature 437, 105–108 (2005).
White, T. D. et al. Ardipithecus ramidus and the paleobiology of early hominids. Science 326, 75–86 (2009).
Henry, A. G. et al. The diet of Australopithecus sediba. Nature 487, 90–93 (2012).
Lucas, P. W., Constantino, P. J. & Wood, B. A. Inferences regarding the diet of extinct hominins: structural and functional trends in dental and mandibular morphology within the hominin clade. J. Anat. 212, 486–500 (2008).
Ungar, P. S. & Grine, F. E. Incisor size and wear in Australopithecus africanus and Paranthropus robustus. J. Hum. Evol. 20, 313–340 (1991).
Lwanga, J. S., Butynski, T. M. & Struhsaker, T. Tree population dynamics in Kibale National Park, Uganda1975-1998. Afr. J. Ecol. 38, 238–247 (2000).
Stewart, F. A. & Piel, A. K. Termite fishing by wild chimpanzees: new data from Ugalla, western Tanzania. Primates 55, 35–40 (2014).
Piel, A. K., Lenoel, A., Johnson, C. & Stewart, F. A. Deterring poaching in western Tanzania: the presence of wildlife researchers. Glob. Ecol. Conserv. 3, 188–199 (2015).
Carlson, B. A., Rothman, J. M. & Mitani, J. C. Diurnal variation in nutrients and chimpanzee foraging behavior. Am. J. Primatol. 75, 342–349 (2013).
Houle, A., Chapman, C. & Vickery, W. Tree climbing strategies for primate ecological studies. Int. J. Primatol. 25, 237–260 (2004).
Lucas, P. W. et al. Measuring the toughness of primate foods and its ecological value. Int. J. Primatol. 33, 598–610 (2011).
Vogel, E. R. et al. Food mechanical properties, feeding ecology, and the mandibular morphology of wild orangutans. J. Hum. Evol. 2014, 110–124 (2014).
Ang, K. Y., Lucas, P. W. & Tan, H. T. W. Novel way of measuring the fracture toughness of leaves and other thin films using a single inclined razor blade. New Phytol. 177, 830–837 (2008).
van Casteren, A., Venkataraman, V., Ennos, A. R. & Lucas, P. W. Novel developments in field mechanics. J. Hum. Evol. 98, 5–17 (2016).
Ennos, A. R. Solid Biomechanics (Princeton University Press, New Jersey, 2012).
Kouakou, C. Y., Boesch, C. & Kuehl, H. Estimating chimpanzee population size with nest counts: Validating methods in Taï National Park. Am. J. Primatol. 71, 447–457 (2009).
Mundry, R. & Oelze, V. M. Who is who matters—the effects of pseudoreplication in stable isotope analyses. Am. J. Primatol. 78, 1017–1030 (2016).
Oelze, V. M. Reconstructing temporal variation in great ape and other primate diets: a methodological framework for isotope analyses in hair. Am. J. Primatol. 78, 1004–1016 (2016).
Carlson, B. A. & Kingston, J. D. Chimpanzee isotopic ecology: a closed canopy C3 template for hominin dietary reconstruction. J. Hum. Evol. 76, 107–115 (2014).
Carlson, B. A. & Crowley, B. E. Variation in carbon isotope values among chimpanzee foods at Ngogo, Kibale National Park and Bwindi Impenetrable National Park, Uganda. Am. J. Primatol. 78, 1031–1040 (2016).
O’Connell, T. C., Hedges, R. E. M., Healey, M. A. & Simpson, A. H. R. W. Isotopic comparison of hair, nail and bone: modern analyses. J. Archaeol. Sci. 28, 1247–1255 (2001).
We thank the Uganda Wildlife Authority and the Uganda National Council for Science and Technology for permission to conduct research in Kibale National Park and the Makerere University Biological Field Station for permission to use the facilities at Ngogo. We also thank the Tanzanian Wildlife Research Institute (TAWIRI) and the Commission for Science and Technology (COSTECH) for permission to conduct research in the Issa valley, Tanzania. We extend thanks to UCSD/Salk Center for Academic Research and Training in Anthropogeny (CARTA) for supporting the GMERC and long-term research in the Issa Valley. We would like to convey our gratitude to the directors and staff at both sites that made the research possible and enjoyable. We thank the PanAf’s Paula Dieguez and Mimi Arandjelovic for their support as data manager and program manager respectively. Many thanks to Erin Wessling and Peter W. Lucas for comments on early drafts of this paper. This research was funded by the Max Planck Weizmann Center for Integrative Archaeology and Anthropology. The Pan African Programme: The Cultured Chimpanzee (PanAf) is generously funded by the Max Planck Society Innovation Fund and Heinz L. Krekeler Foundation.
The authors declare no competing interests.
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van Casteren, A., Oelze, V.M., Angedakin, S. et al. Food mechanical properties and isotopic signatures in forest versus savannah dwelling eastern chimpanzees. Commun Biol 1, 109 (2018). https://doi.org/10.1038/s42003-018-0115-6
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