Acoustic noise and other environmental variables represent potential confounds for animal research. Of relevance to auditory research, sustained high levels of ambient noise may modify hearing sensitivity and decrease well-being among laboratory animals. The present study was conducted to assess environmental conditions in an animal facility that houses nonhuman primates used for auditory research at the Vanderbilt University Medical Center. Sound levels, vibration, temperature, humidity and luminance were recorded using an environmental monitoring device placed inside of an empty cage in a macaque housing room. Recordings lasted 1 week each, at three different locations within the room. Vibration, temperature, humidity and luminance all varied within recommended levels for nonhuman primates, with one exception of low luminance levels in the bottom cage location. Sound levels at each cage location were characterized by a low baseline of 58–62 dB sound pressure level, with transient peaks up to 109 dB sound pressure level. Sound levels differed significantly across locations, but only by about 1.5 dB. The transient peaks beyond recommended sound levels reflected a very low noise dose, but exceeded startle-inducing levels, which could elicit stress responses. Based on these findings, ambient noise levels in the housing rooms in this primate facility are within acceptable levels and unlikely to contribute to hearing deficits in the nonhuman primates. Our results establish normative values for environmental conditions in a primate facility, can be used to inform best practices for nonhuman primate research and care, and form a baseline for future studies of aging and chronic noise exposure.
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The datasets generated during the current study and that support the findings of this study are available from the corresponding author upon reasonable request.
Addressing The Rising Prevalence of Hearing Loss (World Health Organization, 2018); https://apps.who.int/iris/handle/10665/260336
Rabinowitz, P. M. Noise-Induced Hearing Loss. Am. Fam. Physician 61, 2759–2760 (2000).
Muzet, A. Environmental noise, sleep and health. Sleep Med. Rev. 11, 135–142 (2007).
van Kempen, E. & Babisch, W. The quantitative relationship between road traffic noise and hypertension. J. Hypertens. 30, 1075–1086 (2012).
Hagerman, I. et al. Influence of intensive coronary care acoustics on the quality of care and physiological state of patients. Int. J. Cardiol. 98, 267–270 (2005).
Basner, M. et al. Auditory and non-auditory effects of noise on health. Lancet 383, 1325–1332 (2014).
Nelson, D. I., Nelson, R. Y., Concha-Barrientos, M. & Fingerhut, M. The global burden of occupational noise-induced hearing loss. Am. J. Ind. Med. 48, 446–458 (2005).
National Research Council Guide for the Care and Use of Laboratory Animals 8th edn (National Academies Press, 2011).
Turner, J. G. Noise and vibration in the vivarium: recommendations for developing a measurement plan. J. Am. Assoc. Lab Anim. Sci. 59, 665–672 (2020).
Heffner, H. E. & Heffner, R. S. Hearing ranges of laboratory animals. J. Am. Assoc. Lab Anim. Sci. 46, 20–22 (2007).
Pfingst, B. E., Laycock, J., Flammino, F., Lonsbury-Martin, B. & Martin, G. Pure tone thresholds for the rhesus monkey. Hear. Res. 1, 43–47 (1978).
Dylla, M., Hrnicek, A., Rice, C. & Ramachandran, R. Detection of tones and their modification by noise in nonhuman primates. J. Assoc. Res. Otolaryngol. 14, 547–560 (2013).
Brown, A. M. & Pye, J. D. Auditory sensitivity at high frequencies in mammals. Adv. Comp. Physiol. Biochem. 6, 1–73 (1975).
Sales, G. D., Wilson, K. J., Spencer, K. E. & Milligan, S. R. Environmental ultrasound in laboratories and animal houses: a possible cause for concern in the welfare and use of laboratory animals. Lab Anim. 4, 369–375 (1988).
Reynolds, R. P., Kinard, W. L., Degraff, J. J., Leverage, N. & Norton, J. N. Noise in a laboratory animal facility from the human and mouse perspectives. J. Am. Assoc. Lab Anim. Sci. 49, 592–597 (2010).
Raff, H., Bruder, E. D., Cullinan, W. E., Ziegler, D. R. & Cohen, E. P. Effect of animal facility construction on basal hypothalamic-pituitary-adrenal and renin-aldosterone activity in the rat. Endocrinology 152, 1218–1221 (2011).
Castelhano-Carlos, M. J. & Baumans, V. The impact of light, noise, cage cleaning and in-house transport on welfare and stress of laboratory rats. Lab Anim. 43, 311–327 (2009).
Lauer, A. M., May, B. J., Hao, Z. J. & Watson, J. Analysis of environmental sound levels in modern rodent housing rooms. Lab Anim. 38, 154–160 (2009).
Valero, M. D. et al. Noise-induced cochlear synaptopathy in rhesus monkeys (Macaca mulatta). Hear. Res. 353, 213–223 (2017).
Burton, J. A., Valero, M. D., Hackett, T. A. & Ramachandran, R. The use of nonhuman primates in studies of noise injury and treatment. J. Acoust. Soc. Am. 146, 3770–3789 (2019).
Westlund, K. et al. Physiological and behavioural stress responses in cynomolgus macaques (Macaca fascicularis) to noise associated with construction work. Lab Anim. 46, 51–58 (2012).
Peterson, E. A., Augenstein, J. S., Tanis, D. C. & Augenstein, D. G. Noise raises blood pressure without impairing auditory sensitivity. Science 211, 1450–1452 (1981).
Bliss-Moreau, E. et al. Improving rigor and reproducibility in nonhuman primate research. Am. J. Primatol. 83, e23331 (2021).
Serafin, J. V., Moody, D. B. & Stebbins, W. C. Frequency selectivity of the monkey’s auditory system: psychophysical tuning curves. J. Acoust. Soc. Am. 71, 1513–1518 (1982).
Bohlen, P., Dylla, M., Timms, C. & Ramachandran, R. Detection of modulated tones in modulated noise by non-human primates. J. Assoc. Res. Otolaryngol. 15, 801–821 (2014).
Christison-Lagay, K. L., Bennur, S. & Cohen, Y. E. Contribution of spiking activity in the primary auditory cortex to detection in noise. J. Neurophysiol. 118, 3118–3131 (2017).
Stebbins, W. C., Hawkins, J. E. Jr, Johnson, L. G. & Moody, D. B. Hearing thresholds with outer and inner hair cell loss. Am. J. Otolaryngol. 1, 15–27 (1979).
Liberman, M. C. & Dodds, L. W. Single-neuron labeling and chronic cochlear pathology. III. Stereocilia damage and alterations of threshold tuning curves. Hear. Res. 16, 55–74 (1984).
Kujawa, S. G. & Liberman, M. C. Adding insult to injury: cochlear nerve degeneration after “temporary” noise-induced hearing loss. J. Neurosci. 29, 14077–14085 (2009).
Ryan, A. F., Kujawa, S. G., Hammill, T., Le Prell, C. & Kil, J. Temporary and permanent noise-induced threshold shifts: a review of basic and clinical observations. Otol. Neurotol. 37, 271–275 (2016).
Saunders, J. C., Dear, S. P. & Schneider, M. E. The anatomical consequences of acoustic injury: a review and tutorial. J. Acoust. Soc. Am. 78, 833–860 (1985).
Saunders, J. C., Cohen, Y. E. & Szymko, Y. M. The structural and functional consequences of acoustic injury in the cochlea and peripheral auditory system: a five year update. J. Acoust. Soc. Am. 90, 136–146 (1991).
Bohne, B. A. & Harding, G. W. Degeneration in the cochlea after noise damage: primary versus secondary events. Am. J. Otol. 21, 505–509 (2000).
Turner, J. G., Bauer, C. A. & Rybak, L. P. Noise in animal facilities: why it matters. J. Am. Assoc. Lab. Anim. Sci. 46, 10–13 (2007).
Youssef, P. N., Sheibani, N. & Albert, D. M. Retinal light toxicity. Eye 25, 1–14 (2011).
Drescher, D. G. Effect of temperature on cochlear responses during and after exposure to noise. J. Acoust. Soc. Am. 59, 401–407 (1976).
Yang, C. H. et al. Constant light dysregulates cochlear circadian clock and exacerbates noise-induced hearing loss. Int. J. Mol. Sci. 21, 7535 (2020).
Rocchi, F., Dylla, M. E., Bohlen, P. A. & Ramachandran, R. Spatial and temporal disparity in signals and maskers affects signal detection in non-human primates. Hear. Res. 344, 1–12 (2017).
Burton, J. A., Dylla, M. E. & Ramachandran, R. Frequency selectivity in macaque monkeys measured using a notched-noise method. Hear. Res. 357, 73–80 (2018).
Rocchi, F. & Ramachandran, R. Neuronal adaptation to sound statistics in the inferior colliculus of behaving macaques does not reduce the effectiveness of the masking noise. J. Neurophysiol. 120, 2819–2833 (2018).
Rocchi, F. & Ramachandran, R. Foreground stimuli and task engagement enhance neuronal adaptation to background noise in the inferior colliculus of macaques. J. Neurophysiol. 124, 1315–1326 (2020).
Peacock, J. et al. The binaural interaction component in rhesus macaques (Macaca mulatta). eNeuro. 8, 402–421 (2021).
Mackey, C. A. et al. Correlations between cochlear pathophysiology and behavioral measures of temporal and spatial processing in noise exposed macaques. Hear. Res. 401, 108156 (2021).
Hauser, S. N., Burton, J. A., Mercer, E. T. & Ramachandran, R. Effects of noise overexposure on tone detection in noise in nonhuman primates. Hear. Res. 357, 33–45 (2018).
Burton, J. A., Mackey, C. A., MacDonald, K. S., Hackett, T. A. & Ramachandran, R. Changes in audiometric threshold and frequency selectivity correlate with cochlear histopathology in macaque monkeys with permanent noise-induced hearing loss. Hear. Res. 398, 108082 (2020).
MacLean, E. L., Prior, S. R., Platt, M. L. & Brannon, E. M. Primate location preference in a double-tier cage: the effects of illumination and cage height. J. Appl. Anim. Welf. Sci. 12, 73–81 (2009).
Norton, J. N., Kinard, W. L. & Reynolds, R. P. Comparative vibration levels perceived among species in a laboratory animal facility. J. Am. Assoc. Lab. Anim. Sci. 50, 653–659 (2011).
Centers for Disease Control and Prevention (CDC) Criteria for a Recommended Standard: Occupational Noise Exposure, Revised Criteria 1998 (United States Department of Health and Human Services,1998); www.cdc.gov/niosh
Winslow, J. T., Parr, L. A. & Davis, M. Acoustic startle, prepulse inhibition, and fear-potentiated startle measured in rhesus monkeys. Biol. Psychiatry 51, 859–866 (2002).
Milligan, S. R., Sales, G. D. & Khirnykh, K. Sound levels in rooms housing laboratory animals: an uncontrolled daily variable. Physiol Behav. 53, 1067–1076 (1993).
Occupational Safety and Health Administration (OSHA). Occupational Noise Exposure: Proposed Requirements and Procedures. Federal Register, 39 (207: 37773–37784) (1974).
Environmental Protection Agency (EPA) Information on Levels of Environmental Noise Requisite to Protect Public Health and Welfare With an Adequate Margin of Safety (United States Environmental Protection Agency, 1974); https://www.nonoise.org/library/levels74/levels74.htm
Berglund, B., Lindvall, T. & Schwela, D. H. WHO Guidelines for Community Noise (World Health Organization, 1999); http://whqlibdoc.who.int/hq/1999/a68672.pdf
Furman, A. C., Kujawa, S. G. & Liberman, M. C. Noise-induced cochlear neuropathy is selective for fibers with low spontaneous rates. J. Neurophysiol. 110, 577–586 (2013).
Wu, P. Z., O’Malley, J. T., de Gruttola, V. & Liberman, M. C. Primary neural degeneration in noise-exposed human cochleas: Correlations with outer hair cell loss and word-discrimination scores. J. Neurosci. 41, 4439–4447 (2021).
Dobie, R. A. & Humes, L. E. Commentary on the regulatory implications of noise induced cochlear neuropathy. Int. J. Audiol. 56, 74–78 (2017).
Fernandez, K. A. et al. Noise-induced cochlear synaptopathy with and without sensory cell loss. Neuroscience 15, 43–57 (2020).
Loud Noise Can Cause Hearing Loss (Centers for Disease Control and Prevention, 2019); https://www.cdc.gov/nceh/hearing_loss/
Hubrecht, R. C. & Carter, E. The 3Rs and humane experimental technique: implementing change. Animals 9, 754 (2019).
Prescott, M. J., Clark, C., Dowling, W. E. & Shurtleff, A. C. Opportunities for refinement of non-human primate vaccine studies. Vaccines 9, 284 (2021).
Jennings, M. et al. Refinements in husbandry, care and common procedures for non-human primates: ninth report of the BVAAWF/FRAME/RSPCA/UFAW Joint Working Group on Refinement. Lab. Anim. 43, 1–47 (2009).
Luchins, K. R., Baker, K. C., Gilbert, M. H., Blanchard, J. L. & Bohm, R. P. Manzanita wood: a sanitizable enrichment option for nonhuman primates. J. Am. Assoc. Lab. Anim. Sci. 50, 884–887 (2011).
We thank J. Turner and the team at Turner Scientific for their loan of and assistance with the Sensory Sentinel device. We thank J. Parker and the Vanderbilt University Medical Center Division of Animal Care husbandry staff for their accommodation of this study. This study was supported by NIH R01 DC 015988. J.A.B. was supported by NIH F32 DC 019817, and C.A.M. was supported by F31 DC 019823-01A1.
The authors declare no competing interests.
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McLeod, A.R., Burton, J.A., Mackey, C.A. et al. An assessment of ambient noise and other environmental variables in a nonhuman primate housing facility. Lab Anim 51, 219–226 (2022). https://doi.org/10.1038/s41684-022-01017-9