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Crispants take the spotlight

A new CRISPR/Cas9 method that can generate F0 mutant zebrafish has the potential to cut costs, spare time, and reduce animal use for researchers interested in screening loss-of-function alleles in vivo.

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Fig. 1: Kroll et al.’s F0 knockout strategy involves three synthetic guide RNAs, increasing the odds of generating fully penetrant mutants that can be used to study gene function.


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Correspondence to Wouter Masselink.

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Masselink, W. Crispants take the spotlight. Lab Anim 50, 95–96 (2021).

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