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Know Your Model: A knockout does not always make a null

Creating a null mutation of a gene is a powerful way to examine gene function, but knocking out part of a gene does not always result in a null allele.

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References

  1. 1.

    Capecchi, M. R. Science 244, 1288–1292, https://doi.org/10.1126/science.2660260 (1989).

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Bronson, S. K. & Smithies, O. J. Biol. Chem. 269, 27155–27158 (1994).

    CAS  PubMed  Google Scholar 

  3. 3.

    Bush, S. J., Chen, L., Tovar-Corona, J. M. & Urrutia, A. O. Philos. Trans. R. Soc. B. Biol. Sci. 372, 1713, https://doi.org/10.1098/rstb.2015.0474 (2017).

    Article  Google Scholar 

  4. 4.

    Kochetov, A. V. BioEssays. 30, 683–691, https://doi.org/10.1002/bies.20771 (2008).

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Maekawa, T. et al. J. Biol. Chem. 274, 17813–17819, https://doi.org/10.1074/jbc.274.25.17813 (1999).

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Breitwieser, W. et al. Genes & Dev. 21, 2069–2082, https://doi.org/10.1101/gad.430207 (2007).

    CAS  Article  Google Scholar 

  7. 7.

    Bhoumik, A. et al. Proc. Natl Acad. Sci. USA. 105, 1674–1679, https://doi.org/10.1073/pnas.0706057105 (2008).

    Article  PubMed  Google Scholar 

  8. 8.

    Reimold, A. M., Kim, J., Finberg, R. & Glimcher, L. H. Int. Immunol. 13, 241–248, https://doi.org/10.1093/intimm/13.2.241 (2001).

    CAS  Article  PubMed  Google Scholar 

  9. 9.

    Reimold, A. M. et al. Nature 379, 262–265, https://doi.org/10.1038/379262a0 (1996).

    CAS  Article  PubMed  Google Scholar 

  10. 10.

    Barnier, J. V., Papin, C., Eychène, A., Lecoq, O. & Calothy, G. J. Biol. Chem. 270, 23381–23389, https://doi.org/10.1074/jbc.270.40.23381 (1995).

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Valluet, A. et al. PLoS One. 5, e15272, https://doi.org/10.1371/journal.pone.0015272 (2010).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  12. 12.

    Zhong, J., Li, X., McNamee, C., Chen, A. P., Baccarini, M. & Snider, W. D. Nat. Neurosci. 10, 598–607, https://doi.org/10.1038/nn1898 (2007).

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Wojnowski, L. et al. Nat. Genet. 16, 293–297, https://doi.org/10.1038/ng0797-293 (1997).

    CAS  Article  PubMed  Google Scholar 

  14. 14.

    Pfeiffer, V. et al. PLoS One 8, e58259, https://doi.org/10.1371/journal.pone.0058259 (2013).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  15. 15.

    Galabova-Kovacs, G. et al. Proc. Natl Acad. Sci. USA. 103, 1325–1330, https://doi.org/10.1073/pnas.0507399103 (2006).

    CAS  Article  PubMed  Google Scholar 

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Acknowledgements

MGD is supported by program project grant HG000330 from the National Human Genome Research Institute (NHGRI) of the National Institutes of Health (NIH).

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Correspondence to Susan M. Bello.

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Bello, S.M., Perry, M.N. & Smith, C.L. Know Your Model: A knockout does not always make a null. Lab Anim 49, 59–60 (2020). https://doi.org/10.1038/s41684-020-0486-4

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