A bright fluorescent protein for neuronal imaging

Campbell, B.C. et al. PNAS (2020)

By introducing ‘folding mutations’ into a green florescent protein (GFP) template, a team led by Gregory A. Petsko from Weill Cornell Medicine has developed mGreenLantern (mGL), a fluorescent protein with greater in vivo brightness than enhanced green and yellow fluorescent proteins (eGFP and eYFP).

When virally expressed into mouse brains, mGL improved the visualization of neuronal morphology compared with eYFP. In addition, fluorescence developed four times faster with mGL than with eYFP, thereby considerably reducing the lag time between viral transduction and imaging.

mGL could be useful to replace traditionally used fluorescent proteins in neuroscience studies that require greater in vivo brightness and faster expression.

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Correspondence to Alexandra Le Bras.

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Le Bras, A. A bright fluorescent protein for neuronal imaging. Lab Anim 50, 14 (2021).

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