Abstract
Glucosylceramide (GlcCer) belongs to sphingolipids and is found naturally in plant foods and other sources that humans consume daily. Our previous studies demonstrated that GlcCer prevents inflammatory bowel disease both in vitro and in vivo, whose patients are increasing alarmingly. Although some lipids are vulnerable to oxidation which changes their structure and activities, it is unknown whether oxidative modification of GlcCer affects its activity. In this research, we oxidized GlcCer in the presence of a photosensitizer, analyzed the oxide by mass spectrometric techniques, and examined its anti-inflammatory activity in lipopolysaccharide (LPS)-treated differentiated Caco-2 cells as in vitro model of intestinal inflammation. The results showed that GlcCer is indeed oxidized, producing GlcCer hydroperoxide (GlcCerOOH) as a primary oxidation product. We also found that oxidized GlcCer preserves beneficial functions of GlcCer, suppressing inflammatory-related gene expressions. These findings suggested that GlcCerOOH may perform as an LPS recognition antagonist to discourage inflammation rather than induce inflammation.
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Introduction
Glucosylceramide (GlcCer) is composed of a long-chain base (LCB) with an amide-link fatty acid (i.e., ceramide (Cer)) and a polar head group (i.e., glucose (Glc)), one class of sphingolipids and is found naturally in plant-derived foods. The LCB composition of GlcCer contained in edible plants is diverse and depends on plant species; for instance, the predominant bases of GlcCer are trans-4, cis-8-sphingadienine (d18:24E,8Z) in rice and maize, d18:24E,8E in soybeans, and d18:18Z in wheat and rye1. Several cell culture and animal studies have reported that GlcCer has a certain ability to ameliorate abnormal lipid metabolism and atopic dermatitis2,3,4. Since we consume a certain amount of GlcCer from plant foods such as rice, maize, and other sources, for example, a daily Japanese diet contains approximately 50 mg of GlcCer5,6, it is believed that GlcCer is consumed in the diet can help prevent these diseases7.
Besides the above, to these beneficial functions of GlcCer, our research group speculated if GlcCer might also have additional functions and thus conducted cell culture experiments to explore them. As a model of intestinal inflammation, we treated differentiated human intestinal Caco-2 cells with lipopolysaccharide (LPS) and then with plant GlcCer prepared from maize and found that GlcCer significantly inhibited LPS-induced inflammation8. Together with the results of animal studies9,10, it is suggested that GlcCer ingestion prevents LPS-induced inflammation in the intestine, thereby preventing inflammatory bowel disease (IBD) and the formation of abnormal crypt foci in the colon, a cause of colon cancer, which is a worldwide problem, potentially.
It is well known that lipids are susceptible to oxidization due to their double bond, which might alter their functions. Thus, like other lipids11, GlcCer might undergo oxidation and alter its activities by modifying its structure. Previous studies have reported that GlcCer-related compounds (i.e., galactosylceramide (GalCer) and lactosylceramide (LacCer)) undergo structural modifications due to oxidation12,13. For example, Santinha et al.12 reported that photooxidation of GalCer and LacCer can produce some oxides with hydroperoxyl group. Furthermore, Couto et al.13 showed that radical oxidation of GalCer and LacCer forms oxides with groups such as keto, hydroxy, and hydroperoxyl. We hypothesized that GlcCer, as well as GalCer and LacCer, could undergo oxidative modification to become oxidized GlcCer and thereby lose GlcCer activity (e.g., alleviating inflammatory effects). Alternatively, the exact opposite possibility might be considered. Since certain oxidants are known to have beneficial effects14,15,16 (e.g., oxidized phospholipids have been reported as antagonists of LPS rather than inducers of inflammation17,18,19), oxidation of GlcCer may promote its activity. To the best of our knowledge, no research from such a perspective has been done in the past.
To prove the above hypothesis, in this study, we first attempted to oxidize GlcCer by photooxidation in the presence of a photosensitizer. We then analyzed the oxide by quadrupole time-of-flight mass spectrometry (qTOF-MS) and liquid chromatography-tandem mass spectrometry (LC–MS/MS). The primary oxidation product of GlcCer was identified and collected. Their anti-inflammatory activity in LPS-treated differentiated Caco-2 cells was examined compared to the original GlcCer to determine whether the structure of GlcCer affects its action. This research demonstrated that GlcCer is indeed oxidized, but the modification does not diminish its anti-inflammatory properties. These findings may provide useful information for further utilization of GlcCer as a functional food element in the future.
Experimental section
Chemicals and materials
Rice-derived GlcCer, ≥ 99%(TLC) was obtained from Nippon Flour Mills Co., Ltd., (Atsugi, Japan). Rose bengal (RB) was purchased from Fujifilm Wako Pure Chemical Co. (Osaka, Japan). Human intestinal epithelial (Caco-2) cell models derived from colon carcinoma were purchased from ATCC (Manassas, USA). LPS from E. coli 055, Dulbecco’s modified Eagle medium (DMEM), non-essential amino acid solution (NEAA), penicillin–streptomycin solution (P/S), trypsin, and fatty acid-free bovine serum albumin (BSA) were obtained from Fujifilm Wako Pure Chemical Co. Fetal bovine serum (FBS) was purchased from Gibco (Tokyo, Japan). All other reagents were of the highest grade available.
GlcCer oxidation and qTOF-MS analysis
GlcCer (25 mg) was dissolved in 5 mL ethanol and placed in a glass vial tube to which a photosensitizer (0.05 mg RB) was added. After the glass vial tube was completely closed, the sample solution was incubated at 4 °C for up to 120 h under an 18 W LED light of 50 Klux (10 cm vertically above the glass vial tube). The resulting solution was loaded onto a SepPak Plus QMA cartridge (Waters, Milford, USA), equilibrated with ethanol, unoxidized and oxidized GlcCer were eluted with 5 mL of ethanol; RB was retained on the cartridge. The eluate was evaporated and dissolved in 5 mL methanol. A portion of the solution (10 μL) was then dissolved in 1 mL methanol and infused directly into qTOF-MS (Bruker Daltonics GmbH, Bremen, Germany) at a flow rate of 10 μL/min (Table S1) to ensure the formation of oxidized GlcCer.
LC–MS/MS analysis of oxidized GlcCer samples
One μL from the above 5 mL methanol sample was diluted with 1 mL methanol, and 20 μL of it was analyzed by LC–MS/MS (4000QTRAP (SCIEX, Tokyo, Japan)). Product ion scanning was performed at m/z 824.8 to assess whether oxidized GlcCer (i.e., GlcCer hydroperoxide (GlcCerOOH)) was present in the sample. An ODS column (COSMOSIL 5C18-MS-II, 2.0 × 250 mm, Nacalai Tesque, Kyoto, Japan) was used with methanol/water (95:5, v/v) containing 0.1% formic acid as the mobile phase at a flow rate of 0.2 mL/min. The column temperature was maintained at 40 °C. Other parameters are shown in Table S2. After confirming the presence of GlcCerOOH, each GlcCerOOH isomer was specifically analyzed by LC–MS/MS using multiple reaction monitoring (MRM). The MRM pair for each GlcCerOOH isomer (Table S3) was determined based on the product ion scan data. Analysis conditions were the same as above.
Preparation of the mixture of GlcCerOOH isomers
GlcCer (25 mg) was oxidized for 96 h as described above. This sample was subjected to high-performance liquid chromatography with a UV detector (HPLC–UV) (210 nm), and the GlcCerOOH isomer fraction was collected. ODS column (COSMOSIL 5C18-MS-II, 4.6 × 250 mm, Nacalai Tesque) was used with methanol/water (95:5, v/v) as the mobile phase at a flow rate of 1 mL/min. The column temperature was maintained at 40 °C. The composition of the fractionated GlcCerOOH isomer mixture was examined by LC–MS/MS. The solvent of the GlcCerOOH isomer mixture was evaporated and dissolved in an appropriate amount of ethanol. The total amount of GlcCerOOH isomers in ethanol was measured by ferrous oxidation-xylenol orange (FOX) 2 assay20. The prepared GlcCerOOH isomer mixture was stored in the dark at – 80 °C until used for cell culture experiments.
Cell culture
Human intestinal Caco-2 cells derived from colon carcinoma (ATCC, Manassas, USA) were cultured in DMEM supplemented with 10% heat-inactivated FBS (v/v), 1% P/S (v/v), and 1% NEAA (v/v) in an incubator at 37 °C containing 5% CO2 under humid conditions. Caco-2 cells (1.5 × 106 cells/mL) were continuously passaged every 3–4 days, and the medium was changed every 2 days. Differentiated Caco-2 cells were obtained by seeding Caco-2 cells; when > 90% confluence was reached, the cells were designated as day 0 and cultured for 21 days to induce differentiation. Then, the cells were treated with 50 μg/mL LPS to induce inflammatory responses.
Cell viability
Human intestinal Caco-2 cells were cultured in 96-well plates containing 100 μL of culture medium with 10% FBS until differentiation. Then, the medium of differentiated Caco-2 cells (5.0 × 104 cells/mL) was changed to DMEM containing 0.1% BSA with/without LPS (50 μg/mL) and GlcCer or GlcCerOOH isomers (5, 10, 20, 50 μM). After 48 h of incubation, 10 μL of cell counting kit-8 (CCK-8) solution (Dojindo, Kumamoto, Japan) was added and incubated for another 2 h, and absorbance was measured at 450 nm using a microplate reader21.
RT-qPCR
Differentiated Caco-2 cells (2.5 × 106 cells) were treated with GlcCer or GlcCerOOH isomers (20, 50 μM) either with or without LPS (50 μg/mL) for 24 h. Total mRNA was extracted using a NucleoSpin® RNA (Takara Bio Inc., Shiga, Japan). PrimeScript Master Mix (Takara Bio Inc.) was used to synthesize cDNA from total RNA (500 ng) following the manufacturer’s instructions. PCR amplification was performed with a CFX96 Ex Taq II (Takara Bio Inc.) using gene-specific primers (Sigma-Aldrich, Tokyo, Japan). PCR conditions were 95 °C for 30 s, followed by 40 cycles of 95 °C for 5 s and 60 °C for 30 s. The relative expression of each targeted gene was determined using the 2−ΔΔCT method with the housekeeping gene (glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) as the reference gene. The primers used in the real-time quantitative PCR (RT-qPCR) are listed in Table 1.
Apoptosis detection
Caco-2 cells were cultured in eight-well Lab-Tek chamber slides (Thermo Scientific, Waltham, USA) containing 0.5 mL of culture medium with 10% FBS until differentiation. Then, the differentiated Caco-2 cells (1.0 × 106 cells/mL) medium was changed to DMEM with 0.1% BSA containing 20 μM of GlcCer or GlcCerOOH isomers with LPS (50 μg/mL). After 24 h of incubation, apoptotic cells were visualized under a fluorescence microscope using TACS 2 TdT-Flour in situ apoptosis detection kit (TUNEL assay, Trevigen, Gaithersburg, USA), followed by 4′,6-diamidino-2-phenylindole (DAPI) staining22,23. The percentage of apoptotic cells was calculated by dividing the number of apoptotic cells by the total number of cells and multiplying the value by 100. Apoptosis-related genes were evaluated by RT-qPCR as mentioned above.
Cytokine secretion analysis
The cytokine levels of IL-1β, IL-6, and TNF-α in the cell culture supernatant were quantified using an ELISA Max™ Deluxe Set Human IL-1β (Cat No. 437004), IL-6 (Cat No. 430504), and TNF-α (Cat No. 430204) (Biolegend, Way San Diego, USA). Briefly, differentiated Caco-2 cells (2.5 × 106 cells) were incubated with DMEM containing 0.1% BSA with/without LPS (50 μg/mL) and GlcCer or GlcCerOOH isomers (20, 50 μM) for 24 h. Then, the cell media was collected and added to 96-well microplates with the standards, and the assay was performed according to the manufacturer’s protocol. Absorbance was measured at 450 nm and 570 nm using a 96-well microplate reader (Tecan Group Ltd., Switzerland).
Statistical analysis
Data are shown as mean ± standard error (SE). All data were subjected to analyses performed with IBM-SPSS statistic version 26 (IBM SPSS Inc, Chicago, USA). Differences between two groups (control vs. LPS group) determined using Student’s t-test. Differences between among more than three groups were determined using a one-way analysis of variance (ANOVA) with Tukey’s post hoc test. p-Value less than 0.05 was considered statistically significant.
Results and discussion
qTOF-MS and LC–MS/MS analysis of GlcCer oxidation products
GlcCer, a main sphingolipid class contained in plant-based foods24, is shown to impact lipid metabolism positively and exhibits potential benefits in inflammation-related conditions such as inflammatory skin disease2,3,4,8. Previously, oxidation of GlcCer-related compounds (GalCer and LacCer) has been reported12,13. Thus, we hypothesize that GlcCer might undergo oxidation, affecting its functions. Regarding rice-derived GlcCer, which accounts for a significant proportion of the GlcCer humans consume daily, the predominant LCB is d18:24E,8Z. These double bonds would be the target for oxidation. To prove this hypothesis, we subjected rice-derived GlcCer to photooxidation in the presence of photosensitizer to determine whether the ene-reaction of GlcCer with singlet oxygen yields oxides such as hydroperoxide. In general, this ene-reaction has the advantage over radical oxidation in that it does not require higher temperatures and avoids reactions that would decompose the generated oxides.
The samples before and after the photooxidation were measured by qTOF-MS (positive electrospray ionization (ESI)). In the Q1 mass spectrum of unoxidized GlcCer, we observed mainly m/z 792.6 [M + Na]+, which is considered the molecular species of d18:24E,8Z/20h:0. The m/z 820.6, 838.6, and 866.6 were considered to be molecular species of d18:24E,8Z/22h:0, t18:18Z/22h:0, and t18:18Z/24h:0, respectively (Fig. 1A). These observations were in agreement with the previous reports analyzing rice-derived GlcCer25. As we expected, the photooxidation of GlcCer yielded new mass shift ions of + 32 Da at m/z 824.5, 852.6, 870.6, and 898.6 (Fig. 1B), suggesting the formation of hydroperoxides12. However, perhaps these are not hydroperoxides but oxides such as epoxy-hydroxides, dihydroxides, and keto derivatives13,26,27 (especially the ions at m/z 852.6 and the other m/z 880.6 may have keto groups). Therefore, to confirm the formation of GlcCerOOH, we focused on the m/z 824.5 ion that was observed abundantly in the Q1 mass spectrum (Fig. 1B) and performed its product ion analysis using LC–MS/MS.
With regard to product ion analysis, our previous LC–MS/MS research demonstrated that collision-induced dissociation (CID) of sodium adduct of lipid hydroperoxide provided the selective fragmentation of hydroperoxyl group using phospholipid28,29,30 and triacylglycerol31. Accordingly, the LC–MS/MS method using sodium was considered to be useful in the structural analysis of GlcCerOOH in terms of investigating the presence or absence of hydroperoxyl groups and binding positions with high precision. When the sample of photooxidized GlcCer was analyzed by LC–MS/MS, two peaks were observed at 14.04 and 16.08 min in the product ion chromatogram of m/z 824.8 [M + Na]+ (Fig. 2A). The mass spectra of the first peak showed that main fragment ions were based on neutral loss (NL) of 156 Da and 185 Da (Fig. 2B). Considering our previous studies, which demonstrated sodium-induced α-cleavage to hydroperoxyl group32, the first peak would be GlcCerOOH bearing hydroperoxyl group at the C8 position (i.e., 8-OOH-GlcCer). On the other hand, the second peak at 16.08 min was predominantly composed of NL of 144 Da (Fig. 2C). Therefore, the second peak was considered to be isomer bearing at the C9 position (i.e., 9-OOH-GlcCer) (Fig. 2A), which agreed with the fact that the fragmentation between C9-C10 is more preferred rather than that of C8-C9. In addition, both GlcCerOOH isomers (8-OOH-GlcCer and 9-OOH-GlcCer) showed an NL of the Glc residue (− 180 Da) in the cleavage of a glycosidic bond (m/z 460.7 and 488.8 for 8-OOH-GlcCer, and m/z 500.5 for 9-OOH-GlcCer) (Fig. 2B, C)33,34. By using LC–MS/MS with MRM analysis, we could selectively detect both 8-OOH-GlcCer (m/z 824.8/638.4) and 9-OOH-GlcCer (m/z 824.8/680.7) (Fig. 3A, B) and found that 8-OOH-GlcCer and 9-OOH-GlcCer were increased depending on oxidation time and reached the plateau point after 96 h (Fig. 3C, D). To our knowledge, this is the first study to show such a formation profile of GlcCerOOH. Finally, a mixture of GlcCerOOH isomers (i.e., 8-OOH-GlcCer and 9-OOH-GlcCer) was collected using HPLC–UV for the subsequent cell culture study (its purity was confirmed by LC–MS/MS (Fig. S1)).
On the other hand, for reference, despite the fact that electrophilic 1O2 directly reacts with double bond by the ene-reaction and forms hydroperoxides35, we did not detect hydroperoxyl derivatives of GlcCer bearing at 4,5-double bond in LCB. To further validate this observation, we analyzed d-erythro-sphingosine (d18:14E) photooxidized under the same conditions as GlcCer. As well as GlcCer oxidation, the hydroperoxyl derivatives of D-erythro-sphingosine bearing 4,5-double bond were not observed (Fig. S2). Given these results and the results of the previous study36, it is suggested that the presence of the hydroxyl group (C3) in LCB prevents the 1O2 oxidation of its adjacent 4,5-double bond. This result also appears to be the same for radical oxidation13,27.
Comparison of anti-inflammatory activity of GlcCerOOH with unoxidized GlcCer
LPS is known to modify various signal transductions (e.g., inflammatory cytokine expression) via toll-like receptor 4 (TLR4) in intestinal Caco-2 cells, leading to intestinal inflammation37,38. Intestinal inflammation is also known to activate the production of apoptotic proteins and induce apoptosis, which is this phenomenon has been observed in patients with IBD39,40. As mentioned in the introduction, in our previous cell culture studies8,41, we found that treatment of differentiated Caco-2 cells with unoxidized GlcCer along with LPS significantly suppressed LPS-induced inflammation and even apoptosis. As some lipids are vulnerable to oxidation which changes their structure and activities, it was suggested that GlcCer might undergo oxidation and alter its functions by modifying its structure. Generally, oxidation results in the loss of these beneficial properties. Conversely, an increase in activity has been reported in some cases (certain oxidants, such as oxidized phospholipids, prevent LPS-induced inflammation by inhibiting TLR4 activation42). These facts made us interested in whether the anti-inflammatory effects of GlcCer are altered by oxidation. In this study, as described above, we oxidized GlcCer in the presence of a photosensitizer and demonstrated that GlcCer was indeed oxidized by using qTOF-MS and LC–MS/MS. As a result, we obtained the mixture of GlcCerOOH isomers in which hydroperoxyl groups were attached to the double in the LCB of GlcCer (i.e., 8-OOH-GlcCer and 9-OOH-GlcCer). Then, we subsequently compared the anti-inflammatory activity of GlcCerOOH isomers with that of unoxidized GlcCer in differentiated Caco-2 cells treated with LPS to determine whether oxidation alters its actions.
First, we confirmed that treatment of differentiated Caco-2 cells with 0–50 μM GlcCer for 48 h had no effect on cell viability (Fig. 4A). The GlcCerOOH isomers were also found to have no effect on viability (Fig. 4B). Since cytotoxicity has been reported at similar concentrations for other lipid hydroperoxides (e.g., linoleic acid hydroperoxide)43, it is possible that the cytotoxicity of GlcCerOOH is relatively low. Based on these results, we decided to perform the following experiments at concentrations of 0–50 µM GlcCer or GlcCerOOH isomers, where no cytotoxicity was observed. As a result, treatment of differentiated Caco-2 cells with LPS for 48 h significantly reduced cell viability (Fig. 4C, D). We found that not only GlcCer but also GlcCerOOH isomers significantly inhibited the LPS-induced cell injuries, in particularly at 20 μM treatment (Fig. 4C, D). These results clearly indicated that GlcCer does not lose its activity due to oxidation and highlight that oxidized GlcCer is not toxic to cells. Then, the expression of inflammatory genes (i.e., TNF-α, NF-kβ, IL-1β, IL-4, and IL-6), which are known to control the inflammatory response44, were evaluated by RT-qPCR. After LPS treatment for 48 h, a reduction in cell viability was detected. Thus, to identify the pathways of the phenomenon. In this study, we investigated gene expressions at 24 h after treatment. The RT-qPCR data revealed that LPS significantly increased the expression of TNF-α, NF-kβ, IL-1β, IL-4, and IL-6 (Fig. 4E–I), and co-treatment of LPS with GlcCer or GlcCerOOH isomers was able to restore the expression of these genes to near-normal levels (Fig. 4E–I). GlcCerOOH at 20, 50 μM showed the suppression of the inflammatory cytokines in the relative gene expression levels. However, GlcCerOOH at 20 μM demonstrated the most effective suppression of inflammatory cytokines. Meanwhile, GlcCerOOH at 50 μM inhibited some inflammatory cytokines slightly but still established the potential to suppress the inflammation. Therefore, it is essential to highlight that the concentration of GlcCerOOH isomers plays a pivotal role in its impact on inflammatory responses. Furthermore, to confirm its anti-inflammatory activities, cytokine secretion levels in the cell culture supernatant were investigated. The results demonstrated that LPS treatment significantly increased TNF-α, IL-1β, and IL-6 compared to the control group. Meanwhile, co-treatment of LPS with GlcCer and GlcCerOOH isomers significantly decreased the cytokine levels (Fig. 5). These findings suggested that not only GlcCer but also GlcCerOOH isomers suppressed LPS-induced inflammatory stress via downregulation of inflammatory cytokines.
To further understand the anti-inflammatory effects of GlcCer and GlcCerOOH isomers, apoptosis (e.g., formation of apoptotic bodies and typical chromatin) was evaluated by TUNEL assay and DAPI staining. Treatment of differentiated Caco-2 with LPS for 24 h induced apoptosis, as evidenced by a significant increase in the ratio of apoptotic DNA degradation and blebbing of the membrane compared to the control cells (Fig. 6A). As in the experiments on cell viability and inflammatory gene expression, co-treatment of LPS with 20 μM GlcCer or GlcCerOOH isomers significantly decreased the number of apoptotic cells (Fig. 6B). Additionally, apoptosis-related gene expression (i.e., COX-2, p21, and p53) were evaluated and results showed that GlcCer and GlcCerOOH isomers also suppressed LPS-induced COX-2, p21, and p53 expression (Fig. 6C–E).
Generally, oxidized lipids are considered to be harmful and induce proinflammatory, whereas some lipids exhibit protective effects; cells and tissues respond to some oxidized lipids with activation of anti-inflammatory processes17,18,19. In this study, we investigated the direct effect of GlcCer and GlcCerOOH on intestinal epithelium and provided evidence that GlcCerOOH isomers suppress inflammatory gene expressions to exhibit protective properties in LPS-stimulated inflammation. An important anti-LPS mechanism is the mutually exclusive binding (antagonism) with TLR4 and its accessory proteins (e.g., MD-2, CD14, and LBP). Past studies indicated that lipid-A-like LPS45 and oxidized phospholipids17,18,19,46 could act as antagonists of TLR4 and suppress the subsequent occurrence of inflammatory stress. Gangliosides, which are glycosphingolipids, were reported to act as antagonists of TLR447. Hence, GlcCer and GlcCerOOH isomers, which are one type of glycosphingolipids, may act as antagonists of TLR4 and suppress LPS-induced inflammation. Moreover, Bochkov et al.17 reported that the LPS inhibition was selective for oxidized phospholipids but not for unoxidized phospholipids. Also, aldehydes (i.e., 4-hydroxynonenal), which are one of the end lipid peroxidation products, could prevent activation of the NF-kB pathway and IL-1β by LPS48. Furthermore, our previous study revealed that higher polarity of sphingolipids showed a more potent anti-inflammatory effect41; the oxidation attaches a hydroperoxyl group into GlcCer to increase the polarity and may even enhance anti-inflammatory properties. Additionally, a previous study demonstrated that inflammatory stress stimulates sphingolipid metabolism and protects intestinal cells through the use of these metabolites23. GlcCerOOH, which has a strange structure for intestinal cells, may be strongly and quickly metabolized compared to GlcCer. Thus, it is thought that the anti-inflammatory mechanism of GlcCerOOH in intestinal epithelium is by action of GlcCerOOH itself and/or its metabolites.
Overall, these suggested the advantages of GlcCerOOH isomers to mitigate inflammatory responses, and the chemical structure modification of lipids, including GlcCer by oxidation, is possible to control their functions. However, actual intestinal inflammation is related to tight junctions, immune cells, and secretion of mucin and IgA, and GlcCer was reportedly associated with tight junctions and immune cells49,50. Therefore, more detailed in vitro and in vivo mechanism analysis will be necessary. Further experiments would be required to confirm this exact mechanism, including epidemiological studies in the future.
Conclusions
Our study demonstrates the oxidation of GlcCer in the presence of a photosensitizer, producing the GlcCerOOH isomers as the major primary oxidation products. Specifically, GlcCerOOH isomers exhibited inhibitory effects on LPS-induced inflammation, suggesting their potential role as antagonists in impeding LPS recognition and thereby attenuating inflammation. This emphasizes the significance of structure–function relationships in oxidized lipids, particularly in modulating their biological activities. These findings are valuable guidance for utilizing GlcCer as a functional food component with potential implications in developing strategies to mitigate inflammatory responses. The elucidation of these mechanisms opens avenues for leveraging GlcCer to control inflammation, thus contributing to the advancement of therapeutic approaches in inflammatory conditions.
Data availability
Raw data were generated at Tohoku University. Derived data supporting the findings of this study are available from the corresponding author on request.
Abbreviations
- BSA:
-
Bovine serum albumin
- CCK-8:
-
Cell counting kit-8
- Cer:
-
Ceramide
- CID:
-
Collision-induced dissociation
- DAPI:
-
4′,6-Diamidino-2-phenylindole
- DMEM:
-
Dulbecco’s modified eagle medium
- ESI:
-
Electrospray ionization
- FBS:
-
Fetal bovine serum
- FOX:
-
Ferrous oxidation-xylenol orange
- GalCer:
-
Galactosylceramide
- GAPDH:
-
Glyceraldehyde-3-phosphate dehydrogenase
- Glc:
-
Glucose
- GlcCer:
-
Glucosylceramide
- GlcCerOOH:
-
Glucosylceramide hydroperoxide
- HPLC:
-
High performance liquid chromatography
- IBD:
-
Inflammatory bowel disease
- IL-1β:
-
Interleukin 1β
- IL-6:
-
Interleukin 6
- LacCer:
-
Lactosylceramide
- LCB:
-
Long-chain base
- LC–MS/MS:
-
Liquid chromatography-tandem mass spectrometry
- LPS:
-
Lipopolysaccharide
- MRM:
-
Multiple reaction monitoring
- NEAA:
-
Non-essential amino acid solution
- NL:
-
Neutral loss
- p21:
-
Cyclin-dependent kinase inhibitor
- P/S:
-
Penicillin–streptomycin solution
- p53:
-
Tumor protein p53
- qTOF-MS:
-
Quadrupole time-of-flight mass spectrometry
- RB:
-
Rose Bengal
- RT-qPCR:
-
Real-time quantitative PCR
- TLR4:
-
Toll-like receptor 4
- TNF-α:
-
Tumor necrosis factor α
- TUNEL:
-
Terminal deoxynucleotidyl transferase dUTP nick end labeling
References
Aida, K. et al. Properties and physiological effects of plant cerebroside species as functional lipids. Adv. Res. Plant Lipids 2015, 233–236 (2015).
Yunoki, K. et al. Dietary sphingolipids ameliorate disorders of lipid metabolism in zucker fatty rats. J. Agric. Food Chem. 58(11), 7030–7035 (2010).
Kohyama-Koganeya, A., Nabetani, T., Miura, M. & Hirabayashi, Y. Glucosylceramide synthase in the fat body controls energy metabolism in Drosophila. J. Lipid Res. 52(7), 1392–1399 (2011).
Duan, J. et al. Dietary sphingolipids improve skin barrier functions via the upregulation of ceramide synthases in the epidermis. Exp. Dermatol. 21(6), 448–452 (2012).
Sugawara, T. & Miyazawa, T. Separation and determination of glycolipids from edible plant sources by high-performance liquid chromatography and evaporative light-scattering detection. Lipids 34(11), 1231–1237 (1999).
Yunoki, K. et al. Analysis of sphingolipid classes and their contents in meals. Biosci. Biotechnol. Biochem. 72(1), 222–225 (2008).
Vesper, H. et al. Sphingolipids in food and the emerging importance of sphingolipids to nutrition. J. Nutr. 129(7), 1239–1250 (1999).
Yamashita, S., Seino, T., Aida, K. & Kinoshita, M. Effects of plant sphingolipids on inflammatory stress in differentiated Caco-2 Cells. J. Oleo Sci. 66(12), 1337–1342 (2017).
Arai, K. et al. Effects of dietary plant-origin glucosylceramide on bowel inflammation in DSS-treated mice. J. Oleo Sci. 64(7), 737–742 (2015).
Yamashita, S., Sakurai, R., Hishiki, K., Aida, K. & Kinoshita, M. Effects of dietary plant-origin glucosylceramide on colon cytokine contents in DMH-treated mice. J. Oleo Sci. 66(2), 157–160 (2017).
Angeli, J. P. et al. Lipid hydroperoxide-induced and hemoglobin-enhanced oxidative damage to colon cancer cells. Free Radic. Biol. Med. 51(2), 503–515 (2011).
Santinha, D. et al. Evaluation of the photooxidation of galactosyl- and lactosylceramide by electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom. 28(21), 2275–2284 (2014).
Couto, D. et al. Glycosphingolipids and oxidative stress: Evaluation of hydroxyl radical oxidation of galactosyl and lactosylceramides using mass spectrometry. Chem. Phys. Lipids 191, 106–114 (2015).
Podrez, E. A. et al. Identification of a novel family of oxidized phospholipids that serve as ligands for the macrophage scavenger receptor CD36. J. Biol. Chem. 277(41), 38503–38516 (2002).
Podrez, E. A. et al. A novel family of atherogenic oxidized phospholipids promotes macrophage foam cell formation via the scavenger receptor CD36 and is enriched in atherosclerotic lesions. J. Biol. Chem. 277(41), 38517–38523 (2002).
Serbulea, V., DeWeese, D. & Leitinger, N. The effect of oxidized phospholipids on phenotypic polarization and function of macrophages. Free Radic. Biol. Med. 111, 156–168 (2017).
Bochkov, V. N. et al. Protective role of phospholipid oxidation products in endotoxin-induced tissue damage. Nature 419(6902), 77–81 (2002).
Oskolkova, O. V. et al. Oxidized phospholipids are more potent antagonists of lipopolysaccharide than inducers of inflammation. J. Immunol. 185(2), 7706–7712 (2010).
Erridge, C., Kennedy, S., Spickett, C. M. & Webb, D. J. Oxidized phospholipid inhibition of toll-like receptor (TLR) signaling is restricted to TLR2 and TLR4: Roles for CD14, LPS-binding protein, and MD2 as targets for specificity of inhibition. J. Biol. Chem. 283(36), 24748–24759 (2008).
Nourooz-Zadeh, J. Ferrous ion oxidation in presence of xylenol orange for detection of lipid hydroperoxides in plasma. Methods Enzymol. 300, 58–62 (1999).
Gan, Z. S. et al. Iron reduces M1 macrophage polarization in RAW264.7 macrophages associated with inhibition of STAT1. Mediat. Inflamm. 2017, 8570818 (2017).
Nguma, E. et al. Ethanolamine plasmalogen suppresses apoptosis in human intestinal tract cells in vitro by attenuating induced inflammatory stress. ACS Omega 6(4), 3140–3148 (2021).
Yamashita, S., Soga, M., Nguma, E., Kinoshita, M. & Miyazawa, T. Protective mechanism of rice-derived lipids and glucosylceramide in an in vitro intestinal tract model. J. Agric. Food Chem. 69(35), 10206–10214 (2021).
Yamashita, S., Kinoshita, M. & Miyazawa, T. Dietary sphingolipids contribute to health via intestinal maintenance. Int. J. Mol. Sci. 22(13), 7052 (2021).
Sugawara, T. et al. Analysis of chemical structures of glucosylceramides from rice and other foodstuffs. J. Nutr. Sci. Vitaminol. 65, 228–230 (2019).
Giuffrida, F., Destaillats, F., Skibsted, L. H. & Dionisi, F. Structural analysis of hydroperoxy- and epoxy-triacylglycerols by liquid chromatography mass spectrometry. Chem. Phys. Lipids 131(1), 41–49 (2004).
Melo, T., Maciel, E., Oliveira, M. M., Domingues, P. & Domingues, M. R. M. Study of sphingolipids oxidation by ESI tandem MS. Eur. J. Lipid Sci. Technol. 114, 726–732 (2012).
Kato, S. et al. Liquid chromatography-tandem mass spectrometry determination of human plasma 1-palmitoyl-2-hydroperoxyoctadecadienoyl-phosphatidylcholine isomers via promotion of sodium adduct formation. Anal. Biochem. 471, 51–60 (2015).
Nakagawa, K., Kato, S. & Miyazawa, T. Determination of phosphatidylcholine hydroperoxide (PCOOH) as a marker of membrane lipid peroxidation. J. Nutr. Sci. Vitaminol. 61, 78–80 (2015).
Kato, S. et al. Evaluation of the mechanisms of mayonnaise phospholipid oxidation. J. Oleo Sci. 66(4), 369–374 (2017).
Kato, S. et al. Determination of triacylglycerol oxidation mechanisms in canola oil using liquid chromatography–tandem mass spectrometry. NPJ Sci. Food 2(1), 1–11 (2018).
Kato, S. et al. Structural analysis of lipid hydroperoxides using mass spectrometry with alkali metals. J. Am. Soc. Mass Spectrom. 32(9), 2399–2409 (2021).
Tudella, J. et al. Oxidation of mannosyl oligosaccharides by hydroxyl radicals as assessed by electrospray mass spectrometry. Carbohydr. Res. 346(16), 2603–2611 (2011).
Moreira, A. S. et al. Neutral and acidic products derived from hydroxyl radical-induced oxidation of arabinotriose assessed by electrospray ionisation mass spectrometry. J. Mass Spectrom. 49(4), 280–290 (2014).
Choe, E. & Min, D. B. Mechanisms and factors for edible oil oxidation. Compr. Rev. Food Sci. Food Saf. 5(4), 169–186 (2006).
Zhou, Y., Park, H., Kim, P., Jiang, Y. & Costello, C. E. Surface oxidation under ambient air-not only a fast and economical method to identify double bond positions in unsaturated lipids but also a reminder of proper lipid processing. Anal. Chem. 86(12), 5697–5705 (2014).
Guo, S., Al-Sadi, R., Said, H. M. & Ma, T. Y. Lipopolysaccharide causes an increase in intestinal tight junction permeability in vitro and in vivo by inducing enterocyte membrane expression and localization of TLR-4 and CD14. Am. J. Pathol. 182(2), 375–387 (2013).
Nighot, M. et al. Lipopolysaccharide-induced increase in intestinal epithelial tight permeability is mediated by Toll-Like Receptor 4/Myeloid differentiation primary response 88 (MyD88) activation of myosin light chain kinase expression. Am. J. Pathol. 187(12), 2698–2710 (2017).
Fulda, S., Gorman, A. M., Hori, O. & Samali, A. Cellular stress responses: Cell survival and cell death. Int. J. Cell Biol. 2010, 214074 (2010).
Subramanian, S., Geng, H. & Tan, X. Cell death of intestinal epithelial cells in intestinal diseases. HHS Public Access 72(3), 308–324 (2020).
Jutanom, M. et al. Effects of sphingolipid fractions from golden oyster mushroom (Pleurotus citrinopileatus) on apoptosis induced by inflammatory stress in an intestinal tract in vitro model. J. Oleo Sci. 69(9), 1087–1093 (2020).
Von Schlieffen, E. et al. Multi-hit inhibition of circulating and cell-associated components of the Toll-like receptor 4 pathway by oxidized phospholipids. Arterioscler. Thromb. Vasc. Biol. 29(3), 356–362 (2009).
Pacifici, E. H. K., Mcleod, L. L., Peterson, H. & Sevanien, A. Linoleic acid hydroperoxide-induced peroxidation of endothelial cell phospholipids and cytotoxicity. Free Radic. Biol. Med. 17(4), 285–295 (1994).
Vitkus, S. J. D., Hanifin, S. A. & McCee, D. W. Factors affecting Caco-2 intestinal epithelial cell interleukin-6 secretion. Vitr. Cell. Dev. Biol. 34(8), 660–664 (1998).
Christ, W. J. et al. E5531, a pure endotoxin antagonist of high potency. Science 268(5207), 80–83 (1995).
Chu, L. H. et al. The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages. Nat. Commun. 9(996), 1–16 (2018).
Kanoh, H. et al. Homeostatic and pathogenic roles of GM3 ganglioside molecular species in TLR4 signaling in obesity. EMBO J. 39(12), e101732 (2020).
Page, S. et al. 4-Hydroxynonenal prevents NF-kappaB activation and tumor necrosis factor expression by inhibiting IkappaB phosphorylation and subsequent proteolysis. J. Biol. Chem. 274(17), 11611–11618 (1999).
Patterson, L. et al. Glucosylceramide production maintains colon integrity in response to Bacteroides fragilis toxin-induced colon epithelial cell signaling. FASEB J. 34(12), 15922–15945 (2020).
Komuro, M. et al. Glucosylceramide in T cells regulates the pathology of inflammatory bowel disease. Biochem. Biophys. Res. Commun. 9(599), 24–30 (2022).
Acknowledgements
We thank Dr. Kazuhiko Aida (Nippon Flour Mills Co., Ltd., Atsugi, Japan) for providing the GlcCer sample and Dr. Yurika Otoki and Dr. Junya Ito (Tohoku University, Sendai, Japan) for their valuable suggestions.
Funding
This work was supported in part by KAKENHI (Grant Number 22H02278 to K.N. and 22K05521 to S.K.) of Japan Society for the Promotion of Science, Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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M.J., S.K., and K.N. conceived the experiments, M.J. conducted the experiments, M.J., M.T., and S.K. analyzed the results, M.J., S.K., S.Y., M.K., and K.N. wrote the manuscript. All authors reviewed the manuscript.
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Jutanom, M., Kato, S., Yamashita, S. et al. Analysis of oxidized glucosylceramide and its effects on altering gene expressions of inflammation induced by LPS in intestinal tract cell models. Sci Rep 13, 22537 (2023). https://doi.org/10.1038/s41598-023-49521-3
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DOI: https://doi.org/10.1038/s41598-023-49521-3
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