Abstract
To evaluate the safety and effectiveness of epigallocatechin-3-gallate (EGCG) solution treating the acute severe dermatitis in patients receiving radiotherapy. This phase I research enrolled patients with thoracic cancer receiving radiotherapy at Shandong Cancer Hospital and Institute in Shandong, China. EGCG solution was sprayed to the radiation field when grade III radiation-induced dermatitis (RID) first appearance. EGCG concentration escalated from 660 to 2574 μmol/L using modified-Fibonacci dose-escalation. RID and related symptoms were followed up every day. Between March 2021 and November 2021, 19 patients were enrolled in this phase I research. The median dose of grade III RID first observation was 44 Gy (30.6–52 Gy). As the EGCG treatment was performed continuously, all these grade III RID reactions were significantly decreased to grade I or grade II RID at three days after use of EGCG (p < 0.001). Significant relief can be observed in burning sensation (p < 0.001), tractive sensation (p < 0.001), tenderness (p < 0.001), erythema (p < 0.001), itching (p < 0.001) and pain (p < 0.001) after 15 days of EGCG treatment. No radiation therapy delay or interruption for all 19 patients. No adverse events were observed and reported associated with EGCG. The highest dose of this Phase I trial (2574 μmol/L) was recommended for continuous Phase II trial for further evaluation. In this phase I clinical research, use of EGCG solution is safe and can significantly relief grade III RID in patients receiving radiotherapy. Thus, EGCG might be a new choice for acute sever RID.
Trial Registration: ClinicalTrials.gov Identifier: NCT02580279 (Full date of first registration: 12/2014).
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Introduction
Radiation therapy (RT) remains an important treatment of thoracic cancer, such as locally advanced non-small cell lung cancer1 (NSCLC), limited stage small cell lung cancer2 (SCLC), esophageal cancer3, breast cancer4 and mesothelioma. Most of these cancers require radiation therapy as adjuvant therapy, definitive treatment or as palliative intent. Within last 5 years, the technology of radiotherapy has developed rapidly: including Cyberknife5, Image-guided Intensity-modulated Radiotherapy6, Unity MR-Linac7, TomoThreapy8 and even Proton radiotherapy9. These precise radiotherapy technologies make radiation dose escalation possible.
The most common adverse event associated with thoracic cancer radiotherapy is radiation-induced dermatitis10 (RID), especially in supraclavicular region. RID might manifest as an initial transient erythema that can occur within the first 24 h after RT. With the cumulative radiation dose reaches 18–20 Gy, dry desquamation can develop10. While moist desquamation can be seen when RT dose higher than 40 Gy, which is more severe and painful10. What’s worse, infection and ulcer formation can stop RT till the area is able to re-epithelialize and heal11.
There is no standard effectual remedy to severe RID. Our research group previous carried out a phase 2 randomized clinical trial to evaluate the efficacy of epigallocatechin-3-gallate (EGCG) to prevent grade I to II RID of patients with breast cancer12. Results showed that prophylactic use of EGCG solution significantly reduced the incidence and severity of RID12. Thus, EGCG has the potential to be a new choice of treatment severe RID for patients receiving radiotherapy.
However, previous studies12,13 have mainly focused on patients with no skin ulcers below grade 2. Based on the good results of EGCG prevention of severe RID in our previous experiment, we conducted this phase I dose escalation study for patients with grade 3 RID. The safety and efficacy to treat sever RID of different EGCG concentrations were analyzed.
Methods
Epigallocatechin-3-gallate administration
EGCG (purity ≥ 95% by high performance liquid chromatography) was purchased from HEP Biotech Co., Ltd (Ningbo, Zhejiang, China) and freshly dissolved in 0.9% saline solution. The EGCG concentration escalated from 660 μmol/L, 1320 μmol/L, 1980 μmol/L, 2574 μmol/L. EGCG administration was initiated when Grade 3 RID occurred first time during radiation therapy. The solution was sprayed three times a day at 0.05 mL beyond the whole radiation field until 2 weeks after first day use of EGCG. Skin wrinkles in armpits and chest wall, need to be fully stretched and exposed before spraying. Other preventive and treatment agents such as: deodorants, lotions, creams, perfumes, external preparations are not allowed in the radiation field during radiation therapy.
Study design and participants
Our research team prospectively conducted this research at department of thoracic radiotherapy of Shandong Cancer Hospital and Institute in Shandong Province, China. The inclusion criteria included: (1) patients 18 years or older; (2) patients with histologically confirmed breast cancer, lung cancer or esophageal cancer. (3) Patients received definitive radiation therapy in which the target included supraclavicular area. (4) Eastern Cooperative Oncology Group performance status of 0–1. 5. Adequate hematologic, hepatic, and kidney function profile. (5) Chemotherapy, targeted therapy and endocrine therapy are allowed.
Exclusion criteria included: (1) patients with unhealed wounds in the radiation area. (2) Patient has severe uncontrolled systemic diseases, such as acute circulatory diseases; severe pneumonia, etc. (3) Women of childbearing age need to exclude pregnancy or lactation. (4) The patient is allergic to EGCG-related components.
This study has been approved by the Ethics Committee of our research center (Shandong First Medical University Affiliated Cancer Hospital). Informed consent was obtained from all patients and/or their legal guardian(s). All methods were performed in accordance with the relevant guidelines and regulations.
Radiotherapy
Patients were simulated by a big-bore computed tomography. Patient imaging data were transferred to the Eclipse treatment planning system (Eclipse 8.6, Varian Medical Systems) for target delineation and radiation planning. The plan was designed using 6-MV beams with IMRT plan for all patients. The prescribe radiation therapy dose were detailed in results Table 1.
Evaluation of RID and RID-related symptom
RID was evaluated and recorded every day by independent 2 observers according to the RID grade of Radiation Therapy Oncology Group (RTOG).
Radiation Therapy Oncology Group score: Grade Description: 0 No change over baseline;1 Follicular, faint or dull erythema/epilation/dry desquamation/decreased sweating; 2 Tender or bright erythema, patchy moist desquamation/moderate edema; 3 Confluent, moist desquamation other than skin folds, pitting edema 4 Ulceration, hemorrhage, necrosis. Grade 0 is no radiation-induced dermatitis. Grade 1 is considered a mild score. Grade 2 is considered a moderate score, and Grade 3–4 is considered a severe score. RID-related symptoms included erythema, burning feeling, itching, pulling, and pain were also evaluated according to Skin Toxicity Assessment Tool every day.
Safety assessments
Adverse events were evaluated and recorded every three days. All adverse events correlated with EGCG were recorded according to CTCAE, 5.0. These include but are not limited to drug-induced skin rash, nausea and vomiting and other gastrointestinal reactions, fever, palpitation, chest tightness, suffocation and other symptoms. At the same time, we should also distinguish whether the above symptoms are related to EGCG or radiotherapy and chemotherapy.
Statistical analysis
Statistical analysis was performed using SPSS. The correlation between variables was analyzed using the Spearman rank correlation test. Continuous variables were compared using the t-test. All tests were two-sided, and p values of less than 0.05 were considered statistically significant.
Ethical approval
IRB approved Identifier from the Ethics Committee of Shandong First Medical University Affiliated Cancer Hospital.
Results
Between March 2021 and November 2021, 21 patients were enrolled and 2 patients stopped radiation therapy. Data of 19 patients were analyzed in our research (Fig. 1). The clinical characteristics of all eligible patients were summarized in Table 2.
Effect of EGCG for RID
During the observation of all patients, grade I RID occurred in all patients. The earliest appearance time of grade I RID among our sample was two weeks after radiation therapy (20 Gy/10 fractions), with a median grade I RID dose of 34.58 Gy (range 20–41.4 Gy). The last time grade I RID was observed in an esophageal cancer patient at the sixth week with RT dose of 41.4 Gy/23 fractions. The median dose of grade III RID first observation was 44 Gy (range from 30.6 to 52 Gy). Radiation dose and fractions of patients’ first observations of grade III RID were detailed in Table 3. At the point of first observation grade III RID for each patient, EGCG was prescribed for each patient after carefully check by oncology radiologist.
As the EGCG treatment was performed continuously, all these grade III RID reactions were significantly decreased to grade I or grade II RID at 1 weeks after use of EGCG (p < 0.001). The RTOG RIDs were recorded every day from the first usage of EGCG. After 15 days follow up, one patient reported grade II RID and one patient reported grade I RID. Other patients all reported no RID after EGCG treatment (p < 0.001). The decreasing trend of RID was shown in Fig. 2.
Our research team also recorded other related symptoms. Significant relief can be observed in burning sensation (p < 0.001), tractive sensation (p < 0.001), tenderness (p < 0.001), erythema (p < 0.001), itching (p < 0.001) and pain (p < 0.001) at the last follow up of EGCG treatment. Details were shown in Fig. 2. The regression of patient-reported symptoms related to acute skin reactions did not seem to correlate with the onset time and the dose of EGCG. One representative case is shown in Fig. 3. No radiation therapy delay or interruption for all 19 patients.
Safety of EGCG for treatment of grade III RID
The dose of EGCG solution began from 660 to 2574 μmol/L, no adverse events were observed and reported associated with EGCG. And also, no EGCG dose reduction because of adverse events of skin. Even for the skin ulcer after RT, EGCG was well tolerated for all patients. Thus, the highest dose of this Phase I trial (2574 μmol/L) was recommended for continuous Phase II trial for further evaluation.
Discussion
Acute RID usually arise within 90 days of the initiation of therapy with the characteristics of skin dryness, hair loss, hyperpigmentation Dry desquamation, skin scaling and flaking moist desquamation14,15. When the cumulative radiation dose reaches 20–30 Gy, dry desquamation can be observed in patients14,15.
After the cumulative dose for the skin reach to 40 Gy or higher, a more severe RID can be observed in RT filed of skin. Such as edema, fibrinous exudates, and the potential for bullae formation. The damp and tender skin of RT field is painful, which is prone to infection. Radiotherapy may be interrupted due to grade II to III RID14.
No evidence-based standard care has been established for treat RID16. Our research team conducted phase I clinical trial for preventing acute radiation skin adverse events14,15. In this research, EGCG was proved to be safe for patients when the dose of EGCG escalation stopped at 660 mmol/L and no other reported acute toxicity was associated with EGCG. Patient reported RID related symptoms such as pain, burning, itching and tenderness were significantly decreased at 2 weeks after the end of radiotherapy14. Thus, our research concluded that EGCG might be effective in treating acute radiation adverse events. Based on previous researches, our research team conducted a double-blind, placebo-controlled, phase 2 randomized clinical trial to evaluate the efficacy of EGCG in preventing RID in patients with breast cancer receiving postoperative radiotherapy12. The results showed that prophylactic use of EGCG solution significantly reduced the incidence and severity of RID in patients receiving adjuvant radiotherapy for breast cancer.
However, previous studies only enrolled grade I to II RID, and did not included grade III or higher RID of patients during RT12,14,15. There is still no standard treatment for patients with ulcer and skin damage exudation during and after the RT period. Therefore, with the data support of our previous research, our research team further explored whether EGCG is safe and can have expected benefits in treating RID of more than grade III.
Patients received RT field including supraclavicular area or chest wall were observed and enrolled in our research when grade III was observed in those patients. No adverse events associated with EGCG was observed in those patients, even for the ulcer filed of skin. Results showed that symptoms of grade III RID were all reduced and improved significantly after usage of EGCG.
In preclinical trial conducted by professor Zhu found that pretreatment with EGCG significantly enhanced the viability of the human skin cells irradiated by X-rays, and decreased apoptosis induced by X-ray irradiation17. EGCG induced the expression of the cytoprotective molecule hemeoxygenase-1 in a dose-dependent manner via transcriptional activation. That might also be one of the mechanisms of EGCG in treating grade III RID. In our study, results showed that EGCG dose escalating to 2574 mm/L was a safe and effective dose in the treatment of grade III RID.
However, our study still had some limitations. The number of enrolled patients was small. What’s more, the safety and effectiveness need of EGCG still need further evaluation in prospective research.
References
Le Pechoux, C. et al. Postoperative radiotherapy versus no postoperative radiotherapy in patients with completely resected non-small-cell lung cancer and proven mediastinal N2 involvement (Lung ART): An open-label, randomised, phase 3 trial. Lancet Oncol. 23, 104–114 (2022).
Bogart, J. A., Waqar, S. N. & Mix, M. D. Radiation and systemic therapy for limited-stage small-cell lung cancer. J. Clin. Oncol. 40, 661–670 (2022).
Waters, J. K. & Reznik, S. I. Update on management of squamous cell esophageal cancer. Curr. Oncol. Rep. 24, 375–385 (2022).
Kerr, A. J. et al. Adjuvant and neoadjuvant breast cancer treatments: A systematic review of their effects on mortality. Cancer Treat. Rev. 105, 102375 (2022).
Ehret, F. et al. Applications of frameless image-guided robotic stereotactic radiotherapy and radiosurgery in pediatric neuro-oncology: A systematic review. Cancers (Basel) 14, 25 (2022).
Faye, M. D. & Alfieri, J. Advances in radiation oncology for the treatment of cervical cancer. Curr. Oncol. 29, 928–944 (2022).
Lewis, S. et al. Stereotactic body radiation therapy for hepatocellular carcinoma: From infancy to ongoing maturity. JHEP Rep. 4, 100498 (2022).
Forrest, L. et al. Tomotherapy applied total lymphoid irradiation and allogeneic hematopoietic cell transplantation generates mixed chimerism in the rhesus macaque model. Radiat. Res. 196, 623–632 (2021).
Falek, S. et al. Dental management in head and neck cancers: From intensity-modulated radiotherapy with photons to proton therapy. Support Care Cancer 30, 8377–8389 (2022).
Sekiguchi, K. et al. The effectiveness of moisturizer on acute radiation-induced dermatitis in breast cancer patients: A systematic review and meta-analysis. Breast Cancer 20, 22 (2022).
Zhu, W. et al. Epigallocatechin-3-gallate mouthwash protects mucosa from radiation-induced mucositis in head and neck cancer patients: A prospective, non-randomised, phase 1 trial. Invest. New Drugs 38, 1129–1136 (2020).
Zhao, H. et al. Efficacy of epigallocatechin-3-gallate in preventing dermatitis in patients with breast cancer receiving postoperative radiotherapy: A double-blind, placebo-controlled, phase 2 randomized clinical trial. JAMA Dermatol. 158, 779–786 (2022).
Zhu, W. et al. Relationships between the changes of skin temperature and radiation skin injury. Int. J. Hyperthermia 36, 1160–1167 (2019).
Zhao, H. et al. Phase I study of topical epigallocatechin-3-gallate (EGCG) in patients with breast cancer receiving adjuvant radiotherapy. Br. J. Radiol. 89, 20150665 (2016).
Zhu, W. et al. Epigallocatechin-3-gallate ameliorates radiation-induced acute skin damage in breast cancer patients undergoing adjuvant radiotherapy. Oncotarget 7, 48607–48613 (2016).
Miller, R. C. et al. Mometasone furoate effect on acute skin toxicity in breast cancer patients receiving radiotherapy: A phase III double-blind, randomized trial from the North Central Cancer Treatment Group N06C4. Int. J. Radiat. Oncol. Biol. Phys. 79, 1460–1466 (2011).
Zhu, W. et al. Epigallocatechin-3-gallate (EGCG) protects skin cells from ionizing radiation via heme oxygenase-1 (HO-1) overexpression. J. Radiat. Res. 55, 1056–1065 (2014).
Acknowledgements
Certificate of analysis: The ingredient and manufacturing process of EGCG.
Funding
This work was financially supported by the Grants from the National Natural Science Foundation of China (Nos. 82003233, 82172866); Jinan Science and Technology Plan Project (202019163); Science and Technology Project of traditional Chinese Medicine in Shandong Province (2021M013); Bethune Translational Research Foundation of Radiation Oncology (Grant Nos. flzh202125, flzh202124); China Postdoctoral Science Foundation (Grant No. 2022M721446), Shandong Provincial Natural Science Foundation (Grant No. ZR2022MH288), Doctoral foundation of Shandong Natural Science Foundation(No. ZR2019BH067), Shandong Medical Association Clinical Research Foundation (Grant No. YXH2022ZX02151), Shandong Natural Science Foundation Innovation and Development Joint Fund (Grant No. ZR202306010007).
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Study design: H.Z. and X.L. Patients enrollment and treatment: J.X., L.J., X.L., H.Z., P.X., W.Z., L.X., X.M., H.Z. Response evaluation: J.X., H.Z., X.W. Follow up: X.L. and L.J. Data statistical analysis: X.L. and X.Y.
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Xie, J., Jia, L., Xie, P. et al. Efficacy and safety of epigallocatechin-3-gallate in treatment acute severe dermatitis in patients with cancer receiving radiotherapy: a phase I clinical trial. Sci Rep 13, 13865 (2023). https://doi.org/10.1038/s41598-023-40881-4
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DOI: https://doi.org/10.1038/s41598-023-40881-4
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