Declining insect populations emphasize the importance of understanding the drivers underlying reductions in insect fitness. Here, we investigated viruses as a threat to social insect reproduction, using honey bees as a model species. We report that in two independent surveys (N = 93 and N = 54, respectively) of honey bee (Apis mellifera) queens taken from a total of ten beekeeping operations across British Columbia, high levels of natural viral infection are associated with decreased ovary mass. Failed (poor quality) queens displayed higher levels of viral infection, reduced sperm viability, smaller ovaries, and altered ovary protein composition compared to healthy queens. We experimentally infected queens with Israeli acute paralysis virus (IAPV) and found that the ovary masses of IAPV-injected queens were significantly smaller than control queens, demonstrating a causal relationship between viral infection and ovary size. Queens injected with IAPV also had significantly lower expression of vitellogenin, the main source of nutrition deposited into developing oocytes, and higher levels of heat-shock proteins, which are part of the honey bee’s antiviral response. This work together shows that viral infections occurring naturally in the field are compromising queen reproductive success.
Amidst a backdrop of widespread insect declines1,2,3,4,5,6 and fluctuating populations7,8, it is vitally important to better understand the impacts of interacting biotic and abiotic stressors on insect physiology9. Pesticide exposure and climate change are often cited as drivers of insect decline9,10,11,12,13, but biotic drivers, such as viruses, are comparatively understudied, despite some viruses exhibiting broad host ranges14 with the potential for widespread impacts across species. Stressors that reduce fertility are particularly worrisome, especially for social insects, as damage to a single individual (the queen) can weaken the fitness of the entire colony. Additionally, extreme temperatures can have immunomodulatory effects in some insects15,16; therefore, the stressors of climate change and viral infections may actually interact to have synergistic effects, further underscoring the need to better understand the effects of both stressors17.
Viruses that affect managed species like honey bees (Apis mellifera) can infect a broad range of insect host species, creating the potential for pathogen spillover to native species pollinating in the same regions18,19,20,21,22,23,24,25. Infection with Israeli acute paralysis virus (IAPV) and Kashmir bee virus (KBV), which infect both Apis and non-Apis pollinators, results in slower colony start-up and a reduction in egg laying in Bombus terrestris26. Furthermore, high titres of deformed-wing virus (DWV) are associated with extreme cases of ovarian degeneration in honey bee queens27, but there is a need for more research in this area.
Beyond the symptomatic impacts on insect health, virus infections also have the potential to indirectly reduce fecundity, even in the absence of overt symptoms, through a trade-off between reproductive ability and immune activation28. Reproduction and immune processes, whether induced by a pathogen or constitutively maintained, are both energetically demanding. Given a finite resource supply, an individual may not be able to sustain both fully at the same time28. Across multiple insect orders, mated female insects tend to have reduced immunity compared to their unmated counterparts28. Correspondingly, immune-challenged individuals frequently show a reduction in reproductive output measured as reduced overall fecundity29, reduced protein in both ovaries and eggs30,31, reduced oviposition rate31,32, and reduced viability of stored sperm33,34. We have previously shown that lysozyme, an immune effector, is negatively correlated with stored sperm viability in honey bee queens and that failing queens, even without symptoms of viral infection, have significantly lower sperm viability and higher titers of black queen cell virus (BQCV) and sacbrood virus (SBV)35. This suggests that reproduction and immune activation, as a result of pathogenic challenge, are negatively associated in honey bee queens, at least in terms of sperm maintenance. Furthermore, a potential immune-reproduction compromise is particularly relevant for a honey bee queen, whose physiology is tailored for laying eggs and little else; their ovaries make up about one third of their body mass, and they can lay > 1000 eggs a day, which is roughly equivalent to their own body weight36,37. This is a massive investment of resources in a singular reproductive process, contrary to sperm maintenance, which is achieved mainly by enzymatic suppression of reactive oxygen species in the spermatheca and creating the conditions that sustain sperm in a metabolically quiescent state38,39. Indeed, if reproduction-immunity trade-offs apply to sperm storage, as has been suggested in honey bees and other insects28,33,34,35,40, they would most likely be due to collateral damage of immune effectors, such as reactive oxygen species, rather than resource-allocation. Additionally, a queen’s resource investment in ovary size is highly plastic and responds dramatically to external stressors like nutrient availability37. Thus, viral infections could negatively impact fertility either through direct effects of infecting reproductive tissue or compromised allocation of resources associated with systemic infections.
Here, we investigated the relationship between viral infection and reproductive physiology in honey bees, a model social insect. We measured the ovary masses of queens rated as ‘failed’ and ‘healthy’ by beekeepers which had natural variations in viral infection. In two independent populations, we identified and validated a negative relationship between viral infection and ovary size. We measured ovarian protein investment associated with viral infection using quantitative proteomics and confirmed the upregulation of antiviral proteins in virus infected queens. Finally, we have confirmed the causal, rather than correlative, effect of virus infection on ovaries by experimentally infecting queens with IAPV. This work suggests that pathogenic infections negatively impact reproductive quality and fitness in a model insect, supporting the idea that infection may be a significant driver of declining insect health.
Results and discussion
Failed queens have smaller ovaries and reduced sperm viability
In order to investigate if small ovary size is a general feature of queens with poor reproductive output (described by beekeepers as “failing”), we compared the ovary masses of failing and healthy queens collected from beekeeping operations in three independent queen surveys (denoted as field surveys 1–3) conducted in multiple geographical locations (described in Table 1). We found a highly significant reduction in ovary mass (linear mixed model; t = − 4.37, df = 104, p < 0.0001) in failed queens across surveys (Supplementary Table S1 and Fig. 1a), indicating that small ovaries are a widespread phenotype associated with poor fecundity, as described by beekeepers. Whether this is a causal factor or merely a symptom remains unclear, but underscores the relevance of ovary mass as an important queen quality metric.
In our previous queen survey (Survey 1), we also found that queen failure was associated with reduced stored sperm viability and sperm counts (not recorded for Survey 2)35. However, the ages of failed queens were unknown, leading to questions over whether differences in queen quality metrics were linked to queen failure or old age. We therefore collected an additional n = 54 queens (19 failed and 35 healthy), comprising Survey 3, with known ages, the oldest of which were 2 years old, all from our local region in British Columbia to avoid extraneous effects of shipping. We found that sperm viability (generalized linear mixed model, family = binomial; z = − 2.65, p = 0.008) and relative sperm counts (linear mixed model; t = − 2.32, df = 44, p = 0.025) were again significantly lower in failed queens, even when accounting for age as a fixed factor and queen source as a random effect (Fig. 1b–f), corroborating our previous studies35,42. See Table 2 for complete statistical reporting and Supplementary Table S2 for the underlying data. In this survey, failed queens also had significantly smaller ovaries (linear mixed model, t = − 2.33, df = 43, p < 0.023) (Fig. 1g).
Ovary size is inversely linked to viral abundance in naturally infected queens
Queen honey bees are readily infected by many pathogenic agents27,43,44,45, and most commonly infected with sacbrood virus (SBV), black queen cell virus (BQCV), and deformed-wing virus (DWV)35,43. We previously found that the failed queens from Survey 1 have higher copy numbers of SBV and BQCV RNA35.We therefore hypothesized that ovary size is linked to viral load, with the rationale that viral infection could either directly impact ovary function by infecting the tissue, or indirectly impact ovaries by shunting resources into immune activation while depleting resources available to invest in ovarioles.
Using the previously published viral analysis of queens from Survey 1 (n = 93)35, which was acquired by absolute quantification using qPCR and reported as copies per ng of total RNA, we tested if viral load was a predictor of ovary mass. Viral abundance was not significantly associated with the other fertility metrics of sperm viability and sperm counts that we collected (Table 2). A subset of these queens was previously analyzed for 7 of the most common honey bee viruses but only DWV-A (DWV-B was not specifically included), SBV, and BQCV were detected, so the remaining queens were analyzed for just these three. We used two models for analysis: the first with the copies of each individual virus as fixed effects, and the second using a “total viral load” in which the counts of each virus were summed together. We were interested in the effects of an additive total viral load because we hypothesized that the changes in ovary mass are caused by viral infection generally, and not driven by the effects of one particular virus. For both, we used a linear mixed effect model (lme package in R) with queen source (breeder) as a random effect, and queen health status (failing or healthy) and virus counts (separately or added) as fixed effects. See Table 2 for complete statistical reporting and Supplementary Table S3 for the underlying data.
When the counts of each of the three viruses were used as predictors individually (Fig. 2a), we found that only DWV was statistically significant (estimate = − 1.6111, df = 88, t = − 2.146, p = 0.03), although BQCV had a similar effect size (estimate = − 1.2779, df = 88, t = − 1.698, p = 0.09). In the combined model (Fig. 2b) total viral load was significant with a greater effect (estimate = − 2.2598, df = 90.0000, t = − 3.530, p = 0.0007). Figure 2c illustrates the relative proportion of each virus in each queen, which reveals no obviously discernable pattern between each virus and ovary size. Additionally, when comparing the two models using Akaike Information Criterion scores, the total load model is a better fit, indicating that combined viral load is a better predictor of ovary mass than the counts of any one virus. Combined, these illustrate that increased viral load is significantly associated decreased ovary mass, and that this is not obviously due to the effects of one specific virus, but more likely due to viral infection in general.
We then investigated this relationship with the n = 54 queens from Survey 3, which we sampled independently from different apiaries, and whose viral abundances were analyzed by a different laboratory and reported as copies per ng total RNA (data in Supplementary Table S4; Fig. 2d,e). Despite a much smaller sample size, identifying an overall lower range of viral abundance, and not identifying two of the three most common viruses present in queens (BQCV and SBV) (which may be due to different laboratory methods), we observed the same trends as for Survey 1. Though none of these relationships were significant, observing the same correlation in an independent set of samples, with different viruses quantified from a different tissue, provides further evidence that this is a biologically relevant phenomenon. Future studies with larger sample sizes and uniform viral quantitation methods could serve to better illuminate this relationship between natural infections.
To confirm that viral infection is causally associated with the reduced ovary size of failed queens and not merely a result of failed queens potentially being more susceptible to infection, we injected age-matched, mated queens with IAPV. We chose IAPV because it naturally infects honey bee queens, but has a low frequency of infection43, so experimental infection is unlikely to confound with natural abundance. After just 65 h, we found that queens with higher levels of IAPV had significantly smaller ovaries (Fig. 3a) (simple linear model: estimate = − 1.36, t = − 2.51, df = 15, p = 0.024), echoing our results from the field. There appeared to be contamination of the mock-injected queens with IAPV so we have analyzed the data as a continuous relationship, rather than as a comparison between groups for transparency. The number of IAPV copies was confirmed by absolute quantification using qPCR, and the mock-injection queens were additionally checked for DWV, SBV, and BQCV, of which there was no detectable infection in any of the queens.
Two caveats to this experiment should be noted, though. The first is that infection by injection has previously been shown to increase mortality, viral titre, and expression of immune effectors relative to the oral infection route in worker honey bees with BQCV46. This means that the responses we observed here are likely to represent a more extreme response than from other the other routes of transmission (oral, vertical, or sexual), though at this time, evidence for which of these represents the most common “natural” route of infection for adult queens is lacking. Secondly, assessing the mock-injected queens for DWV, SBV, and BQCV allowed us to broadly conclude that the levels of these viruses in this population of queens is low. However, we cannot rule out the possibility that these (or other) viruses may have been present in one or more queens in the IAPV-injection group, which could potentially lead to interactive effects between viruses47.
In a separate experiment, we investigated vitellogenin gene expression in the abdomens of IAPV-injected queens relative to mock-injected queens at three different ages: two days before emergence (pupae), two days after emergence, and two weeks after emergence (with CO2 treatment to stimulate ovary activation and egg laying48). The success of the infections was confirmed by measuring IAPV copies using qPCR (Supplementary File 1). In pupal queens and queens two weeks after emergence (after CO2 ovarian stimulation), we found that expression of vitellogenin was significantly reduced by IAPV infection (Fig. 3b). Vitellogenin, the major egg yolk protein precursor, is one of the most significant sources of nutrition for developing oocytes, where it is deposited in massive quantities. Much of the mass of a queen’s ovary is made up of developing eggs, therefore a decrease in vitellogenin expression is consistent with a reduction in ovary mass and together represents a reduction in the resources being invested into producing or provisioning eggs. Vitellogenin is also responsible for the binding and transport of immune elicitors (i.e., bacterial cell wall components) during transgenerational immune priming49,50. So, this reduction in vitellogenin could have knock-on effects for the success of the queen’s progeny by reducing her capacity to provide them with immune priming.
Ovaries of failed queens have altered protein composition
To determine if the changes we observed in the ovaries of failing queens were associated with altered patterns of protein expression consistent with a disruption in egg production, we performed quantitative proteomics on the ovaries of n = 88 queens collected in British Columbia from Survey 1. We found that 415 proteins, around 20% of the total number of quantified proteins, were differentially expressed between failing and healthy queens at a false discovery rate of 5% (Benjamini Hochberg method; Fig. 4a). Many of the proteins that were downregulated in failing queens are associated with metabolic processes, but no gene ontology (GO) terms were significantly enriched among the differentially expressed proteins. This could be due to the poor GO characterization of many honey bee proteins, as we have previously described35. However, among these differentially expressed proteins are several indicators of an increase in immune activity. One of the top proteins decreased in failed queens was prohibitin (XP_624330.3), a highly multifunctional protein associated with cellular proliferation51, the regulation of follicular development in mammalian ovaries52, and a mediator of viral entry into insect cells53. Furthermore, heat-shock proteins have antiviral activity in insects54,55, and our data are consistent with that role in queens. We found that two small heat shock proteins (protein lethal(2) essential for life), XP_001119884.1 and XP_001120194.1, as well as dnaJ homolog shv (XP_006569897.2) were upregulated in failed queens (Fig. 4b–d). Despite sharing the same name (protein lethal(2) essential for life), these proteins do not share tryptic peptides > 6 residues long; therefore, their quantification was not influenced by shared peptide sequences. DnaJ homolog shv was also previously reported to be associated with the antiviral heat-shock response in workers55.
Studies in Lepidopterans and Orthopterans have shown that the protein apolipophorin III (apoLp-III) is involved in both the immune system and metabolism56. When in its monomeric form, it acts as an immune surveillance pattern-recognition receptor with the ability to activate the immune response. We previously found that apoLp-III is significantly co-expressed with a cluster of predominantly innate immune proteins in the spermathecal fluid35, lending confidence to its involvement in immune processes in queens. But, when combined with high-density apolipophorin (apoLp-I/II), it acts as a lipid transporter, shuttling lipids liberated from the fat body and from the digestive system around the body57. One of the most significant destinations of these lipids in reproductively active female insects are the developing oocytes, where they will make up 30–40% of each egg’s dry weight58. These lipophorins could be a molecular switch governing both the movement of resources and immune function, and we predicted that if increased viral loads are a feature of failed queens, then they should have increased levels of apoLp-III to promote immune function. Additionally, we predicted that apoLp-I/II, which is constitutively involved in lipid transport and juvenile hormone signalling, would be reduced in the smaller ovaries of failing queens which are likely producing fewer eggs. We found that apoLp-III was indeed upregulated in failed queens, and apoLp-I/II was downregulated (Fig. 4e,f), suggesting that lipid transport to developing eggs is reduced in failing queens.
Heat-shock proteins are upregulated in virus-infected queens
To gauge whether the levels of viral infection in the field were sufficient for the queens to launch an antiviral response, we investigated expression of putative antiviral proteins. Virus-infected workers express elevated levels of antimicrobial peptides (e.g., defensin, hymenoptaecin); however, these peptides’ molecular properties are not favourable for quantitation by shot-gun proteomics without specialized enrichment strategies. Additionally, we have not been able to identify or quantify several of the other proteins classically involved in the antiviral response, including Dicer and Argonaute-2, in these or similar samples. Previous work has demonstrated that in fruit flies and worker honey bees, heat-shock proteins, which are more easily quantified by proteomics, are an important part of the antiviral defense54,55,59. Virus-infected workers also upregulate heat-shock protein mRNA (pl(2)el, hsp70-3, hsp70-4, hsp83-like, and hsp90), and workers whose heat-shock proteins were experimentally induced by temperature stress shortly after infection had 74–90% lower viral titers compared to non-induced controls55. We therefore hypothesize that antiviral heat-shock protein expression is also correlated with viral load in queens.
We first analyzed previously published quantitative proteomics data obtained from spermathecal fluid samples of the queens from Survey 1 with varying degrees of viral infection35,41. Honey bee viruses can be transmitted sexually, so spermathecal fluid is a reasonable tissue in which to look for an antiviral response60. Our statistical model included sperm counts, health status, and log-transformed total viral RNA copies as fixed effects. We found that six proteins were non-significantly correlated with log-transformed total viral RNA copy numbers (adjusted p-values between 0.054 and 0.082) (Table 3), half of which are heat-shock proteins: heat-shock protein 70 cognate 4 (HSP70-4) and two other small heat-shock proteins (sHSPs) XP_001120006.2 and XP_001119884.1. As we predicted, all three HSPs positively correlate with viral abundance (HSP70-4 and sHSP XP_001120006.2, which have been previously associated with the worker bee antiviral response55, are depicted in Fig. 5a,b). The other three proteins—a transmembrane protease, zinc carboxypeptidase, and arylsulfatase-B—have not been previously linked to viral infection in honey bees. Notably, the two sHSPs were previously proposed as candidate diagnostic markers for heat-stress and were significantly upregulated in failed queens relative to healthy queens41; here, we show that they are linked to viral abundance even when health status is included as a fixed effect. This observation unfortunately negates their utility as heat stress biomarkers due to confounding virus infection.
To validate that these HSPs are part of the antiviral immune defense in queens we performed experimental infections with IAPV. We selected the two heat shock proteins that we identified as upregulated in naturally virus infected queens that overlapped with the previously reported list of HSPs associated with the antiviral response in workers: protein lethal(2) essential for life (XP_001120006.2, Pl(2)el) and heat-shock protein 70 cognate 4 (Hsp70-4)55 (all primer sequences are available in Supplementary Table S5). Pl(2)el and HSP70-4 were significantly upregulated after viral infection in queens of all three ages, with the exception of two-week-old queens, in which HSP70-4 was not differentially expressed (Fig. 5c,d, Supplementary Table S6). The fact that HSP70-4 was not upregulated in queens whose ovaries had been stimulated to develop may be because insects which have mated or begun to reproduce often demonstrate a reduced ability to mount an immune response relative to their unmated counterparts28, and trade-offs involving the immune response often involve a complicated reconfiguration in which different components of the response might become up or downregulated61.
These data show that viral infection is impacting the fertility, and therefore success, of honey bee queens. Queens collected from the field showed a clear negative relationship between viral counts and ovary mass—a trend which was observed in a second, independent set of queens. Furthermore, experimental infections resulted in a reduction in ovary mass and reduced vitellogenin expression, which suggests a causal, rather than merely correlative role of virus infection. Queens deemed to be failing by beekeepers have increased viral loads and smaller ovaries relative to queens perceived to be healthy. This finding combined with protein expression patterns indicating a decrease in oocyte development and increased antiviral response suggests that this is an economically relevant phenomenon.
Exactly what is responsible for this association between reduced ovary size and viral infections is yet unclear. It could be due to direct impacts of the viral pathology, or some indirect effect such as causing a queen to split resources between reproduction and launching an immune defense. The facts that each virus individually showed a similar effect size on ovary mass from the first survey queens, that an additive total viral count model better predicted ovary mass than each virus separately, and that experimental infections done with a different virus from those which we identified in the field provide evidence that this upregulation of the antiviral response and reduction in reproductive activity are general responses to viral immune activation, and not a specific response to a particular virus. Additionally, ovary mass is a highly plastic parameter—for example, it tends to decline when queens are confined to small cages with only a few attendants during shipping, but it rebounds again after being placed in a queen bank colony for several days or when she reinitiates oviposition41. Therefore, the effects that we observe on ovary size may be temporary and could conceivably be reversed if, for instance, the virus infection was cleared.
The increase in expression of antiviral HSPs we observed in naturally infected queens was recapitulated in queens of different ages experimentally infected with a different virus. Since some of the same antiviral proteins are upregulated with both virus infection and heat-shock, these data also highlight a potential mechanism by which extreme temperatures associated with climate change could have immunomodulatory effects. Whether such an interaction would have positive or negative impacts on reproduction remains to be tested. It is possible that pathogen spillover events, as has been suggested to occur between honey bees and other species, could negatively impact insects not only through direct fitness costs of infection, but through indirect effects on the population via reduced reproductive output.
Materials and methods
For all surveys, beekeepers considered the queens to have ‘failed’ if they either displayed spotty brood patterns, were drone layers, or if the adult population rapidly dwindled, including the presence of chalkbrood infections that the colony could not resolve on their own. Beekeepers considered the queens to be ‘healthy’ if they produced robust brood patterns, the colony did not have signs of appreciable brood infections, and the adult population was strong. Additionally, queens were collected during the summer and beekeepers determined that the colonies were not in the process of gearing up to swarm.
For Survey 1, we utilized previously published data35, which included N = 105 queens (excluding imported queens, which were biased to smaller ovary sizes due to a longer caging duration). N = 93 of these queens had associated viral data. For the ovary mass comparison across surveys, we retained only queens from producers that met the following criteria: the producer collected both failed and healthy queens, queens were delivered to the laboratory in one shipment, and the producer provided n > 2 failed and healthy queens each. These criteria were applied because in the 2019 survey, producers from many different locations shipped queens to the laboratory by different methods and with collection schedules; therefore, queens spent different lengths of time in transit and in cages prior to transit, which we know from previous work impacts ovary mass. Applying these criteria reduced our sample size for the ovary mass comparison but removed these extraneous effects.
For Survey 2, N = 22 queens were collected from colonies in 2018 from three different apiaries in southeastern Pennsylvania. Healthy and failed queens were age- and apiary-matched in order to eliminate sampling bias. The queens were frozen on dry ice immediately in the field and shipped frozen to the University of British Columbia, at which time the queens were thawed on ice and dissected.
For Survey 3, queens were collected from local collaborating beekeepers in British Columbia as they became available during the summer of 2020. Apiary location, colony symptoms (if failed), genetic background (i.e., local or imported stock), and age were recorded. Failed and healthy queens were caged with candy and attendants then kept in a queen bank with plentiful nurse bees if they could not be immediately analyzed in the laboratory. The queens were transported to the University of British Columbia, where they were anesthetized with carbon dioxide, decapitated, and their spermathecae were removed for sperm viability analysis. The remaining queen bodies were stored at − 70 °C until further dissection to determine ovary mass.
Sperm viability, sperm counts, and ovary mass
Sperm viability measurements for Survey 3 queens were obtained essentially as previously described35, following the methods of Collins and Donaghue62. Briefly, we burst the spermatheca with tweezers in 100 µl of Buffer D (17 mM d-glucose, 54 mM KCl, 25 mM NaHCO3, 83 mM Na3C6H5O7) and gently agitated the solution until sperm cells were homogeneously dispersed. We then stained the sperm using SYBR-14/propidium iodide dual fluorescent staining following the manufacturer’s protocol (Live/Dead sperm viability kit, Thermo). We dispensed 5 µl of the stained sperm into a well of a 24-well plate, over which we placed a round glass cover slip. We imaged the sperm (three fields of view per queen, average of ~ 100 sperm cells per image) using a Cellomics ArrayScan XTI (Thermo) and automatically counted live (green) and dead (red) channels using ImageJ v1.52a. Relative sperm abundances were obtained by counting the cumulative number of sperm across three fields of view. Every sample was analyzed in the same style of plate and with the same sized cover slip, so measuring the total sperm in a given area is proportional to total sperm in the sample. We later dissected the ovaries from the queen remains and weighed them using an analytical balance. Data for Survey 1 is already published and we direct the reader to McAfee et al.35 for the methods regarding the collection and processing of those queens.
Viral quantification for queen surveys
Viral data (SBV, DWV-A, and BQCV) for Survey 1 were obtained by RT-qPCR exactly as previously described and are previously published data35. Briefly, samples were submitted to the National Bee Diagnostics Center, where total RNA was extracted from the head using a guanidinium isothiocyanate extraction buffer, cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, USA), and virus RNA was quantified by qPCR with previously published primers35. Standard curves for each virus amplicon were generated using serially diluted plasmids containing the target sequence. RP49 was used as the honey bee reference gene, enabling the viral RNA copy number per 30 ng of total RNA to be calculated. Copy numbers per 30 ng of RNA of all three viruses were added together to generate the ‘Total counts’ variable. All viral count data was then rescaled as counts per µg of extracted RNA in order to have data from both surveys depicted on the same scale, and log10 transformed. Queens from Survey 2 were not analyzed for viral infections because the sample size (22 queens) is expected to be too small to yield sufficient statistical power linking viral abundance to ovary size.
Queens from Survey 3 were processed by the Honey Bee Queen and Disease Clinic at North Carolina State University using methods as previously described63 with minor adjustments. For this sample set, we analyzed thoraxes because we have observed that other components of the head can sometimes interfere with PCR. We also tested for a wider range of viruses, including DWV-A, DWV-B, ABPV, SBV, IAPV, LSV, BQCV, and CBPV. Thoraxes were pulverized by bead beating and total RNA was extracted via the Zymo Research DirectZol RNA miniprep kit and Trizol reagent. RNA was quantified and verified for quality by NanoDrop Spectrophotometry and diluted to 200 ng/µL in RNAse/DNAse free water. cDNA was synthesized using the BioBasic All-In-One RT MasterMix. qPCR was performed on a QuantStudio 5 thermocycler using PowerUp Sybr Green chemistry. Standard curves were generated using serial dilutions of known quantities of custom plasmids containing the target sequences. Actin, 28 s ribosomal subunit, and GapDH were used as reference genes. Final copy numbers were normalized to reference gene values using GeNorm software64 and calculated per 3.33 ng of total RNA, then rescaled to counts per µg total RNA and log10 transformed.
Statistical analyses of phenotypic data
All statistical analyses were performed in R v3.5.1. For Survey 3 data, we first checked if ovary mass, sperm count, and sperm viability were normally distributed and had equal variance between failed and healthy queens using the Shapiro and Levene test, respectively. The ovary mass and sperm viability passed these tests (p > 0.05); therefore, a linear mixed model (lme) was used for both, including health status (failed vs. healthy) and age (0, 1, or 2 years) as fixed effects and location (9 levels) as a random effect. The sperm viability data, however, was not normally distributed, so we analyzed these data with a generalized linear mixed model with a logit link function (glmer, family = binomial) as previously described34, constructed using the same fixed and random factors, but where sperm viability was represented as counts of ‘successes’ (live) and ‘failures’ (dead). Pooled ovary mass data from the three independent queen surveys were analyzed using a linear mixed model (lme) with health status as a fixed effect and queen source location as a random effect.
To determine the relationship between ovary mass and virus infection, we used a linear mixed effect model (R package lme4) fit by restricted maximum likelihood (REML) with queen source (apiary) as a random effect and viral counts (either individually, or as summed total viral load) and health status (healthy vs. failing) as fixed effects. We used the ‘DHARMa’ package for a simulation-based approach to assess the goodness of fit of these models by evaluating the scaled (quantile) residuals and tested the model for typical misspecification problems, such as over/underdispersion and residual spatial and temporal autocorrelation. To compare the individual virus vs. total viral load models we refit them using maximum likelihood (ML) in order to compare Akaike Information Criterion scores. Including source (breeder) as a random effect in the models for Survey 1 produced a singular fit with the breeder variance estimated as zero, but we chose to retain this part of the model because it is still a relevant, structural variable in the data and the differences between ovaries masses from each breeder are nontrivial.
Ovary proteomic sample preparation and statistical analysis
Ovary samples from Survey 1 (N = 88, 5 samples from the original 93 queens were lost during processing) were prepared for mass spectrometry essentially as previously described42. One ovary from each queen was homogenized in a separate 2-ml screw cap tube containing 300 µl of lysis buffer (6 M guanidinium chloride, 100 mM Tris, pH 8.5) and four ceramic beads. The homogenizer (Precellys 24, Bertin Instruments) was set to 6500 s−1 for 30 s: samples were homogenized three times and placed on ice for 1 min between each. Then samples were centrifuged (16,000 relative centrifugal force, 10 min, 4 °C) to remove debris. 100 µL of supernatant was transferred to a new tube and diluted 1:1 with distilled H2O. Protein was precipitated by adding four volumes of ice-cold acetone and incubating overnight at − 20 °C. The precipitated protein was pelleted by centrifugation (6,000 relative centrifugal force, 15 min, 4 °C) and the supernatant was discarded. The protein pellet was washed twice with 500 µl of 80% acetone, then the pellet was allowed to air dry (~ 5 min) before solubilization in 100 µl of digestion buffer (6 M urea, 2 M thiourea). Approximately 20 µg of protein was reduced (0.4 µg dithiothreitol, 20 min), alkylated (2 µg iodoacetamide, 30 min, dark), and digested (0.4 µg Lys-C for 2.5 h, then 0.5 µg trypsin overnight). Digested peptides were acidified with one volume of 1% trifluoroacetic acid and desalted with high-capacity STAGE tips as previously described65. Eluted samples were dried (SpeedVac, Eppendorf, 45 min) and resuspended in Buffer A (0.1% formic acid, 2% acetonitrile), then peptide concentrations were determined using a NanoDrop (Thermo, 280 nm). LC–MS/MS data acquisition was performed as previously described42.
MS data was searched using MaxQuant (v18.104.22.168) with the parameters and database as previously described42. In Perseus (v 22.214.171.124), the resulting protein groups were filtered to remove reverse hits, contaminants, proteins identified only by site, and proteins identified in fewer than 10 samples. Normalized LFQ intensity was then log2 transformed. Subsequent analyses were performed in R (v3.6.3). Differential expression analysis was performed using the limma package in R66 with ovary mass, status (failed vs. healthy), and total viral counts (log10 transformed of combined RT-qPCR counts, + 1 to avoid undefined terms) included as fixed effects. Empirical Bayes moderation of the standard errors was then performed on the resulting linear model, and finally the false discovery rate was controlled to 5% using the Benjamini–Hochberg correction. GO term enrichment analysis was performed on the proteins differentially expressed among status groups using Ermine J as previously described41, though no terms were significant. We used a linear model with ovary mass, status, and total viral counts as fixed effects to analyze the expression of ApoLp-I/II and ApoLp-III.
Statistical analysis of spermathecal proteomic data
We analyzed previously published proteomics data35 using the limma R package essentially as previously described for ovaries. Proteomics data were first groomed to remove samples that did not have associated viral abundance data. We included log10 transformed total viral load (+ 1 to avoid undefined terms), health status (failed or healthy), and sperm counts as fixed effects in the statistical model. We used a Benjamini–Hochberg correction to control the false discovery rate 5%. We direct the reader to McAfee et al.35 for details on proteomics sample preparation and upstream data processing.
IAPV experimental infections for measuring ovary mass
Mated queens of approximately the same age (< 1 year old, roughly ~ 1–2 months) were obtained in a single shipment from California. Queens were anesthetized with CO2 for 6 min before being injected between the abdominal tergites with approximately 4nL of IAPV-inoculum containing ~ 1400 copies of IAPV (determined by absolute quantification using qPCR) (n = 10) or phosphate buffer saline (PBS) as mock (n = 10). Injections were performed using the FemoJet Microinjector (Eppendorf) with hand-pulled glass needles. The queens were then kept in wooden cages with 5 attendant workers and provided with sugar candy in the incubator at 34 °C with a water pan for humidity. After 65 h the queens were sacrificed, and the wet ovary masses measured on an analytical balance. IAPV levels were quantified in the heads of all queens by the National Bee Diagnostic Center using the same methods as described above for the viral analysis of the queen surveys. The mock-injected queens were additionally analyzed for DWV, SBV, and BQCV.
IAPV experimental infections for expression analysis
Three large groups of sister queens were produced from a healthy mother queen to study the direct effect of virus infection on queens of three different ages following honey bee queen-rearing technique67. Briefly, young larvae (12–24 h old) were grafted into artificial queen cups and placed in a populous but queenless nurse colony to develop. Prior to grafting, the donor colony was visually determined to be free of symptomatic diseases (Nosemosis, Varroosis, European foulbrood, American foulbrood, and Chalkbrood). After 6 days, sealed queen cells were transferred to an incubator (35 °C, 65% humidity) and treated as follow:
The first group was used for pupal infections. Two days before emergence, queen pupae were gently removed from their capped cells. These pupae were either injected with 2 µL of IAPV-inoculum containing 5.24 × 103 virus particles (n = 34), or phosphate buffered saline (PBS) as a mock control (n = 31). Injections were performed using a Nanojet™ syringe pump (Chemix, USA) with an infusion flow rate of 0.1 µL/s, where the needle was inserted into the first abdominal segment located immediately behind the metathorax. Each queen then was placed individually in a 15 mm well in the 24 well glass bottom cell culture plate and kept in the incubator (35 °C, 65% humidity). While in the incubator, development of the virus injected queens was compared with the PBS injected queens. Our observations confirmed a complete cessation of development in IAPV injected queens 20 h post injection, which was not observed in the control group. Therefore, the experiment was terminated, and the abdomen of each queen was then separated, placed in an Eppendorf tube, and stored in a freezer at − 80 °C until RNA extraction.
The second group of queens was used for infecting newly emerged queen in the pre-reproductive stage. Two days after emergence, queens were either injected with the IAPV-inoculum (n = 30), or PBS as mock control (n = 30) as explained above. Each queen was accompanied with 10–15 worker honey bees from the donor colony in a single-use plastic cup, provided with water and sugar candy. Cups of IAPV-inoculated and PBS-injected groups were maintained separately in the incubator (35 °C, 65% humidity). The IAPV-inoculated queens started showing paralysis symptoms around 20–24 h post injection. The experiment was terminated at the onset of symptoms, and after anesthetization using CO2 the queens’ abdomens were sampled and stored as described above.
For infection of the last group of reproductive queens, right after emergence, queens were caged and banked in a donor colony for one week. Then, they were taken out and treated with CO2 two times a week later and return to the donor colony for one more week to stimulate ovary development without mating48. Thereafter, queens were either injected with the IAPV-inoculum (n = 34), or PBS as mock control (n = 31) and maintained with attendant workers in the incubator (35 °C, 65% humidity), as described above. Paralysis symptoms started in the IAPV-inoculated queens 40–44 h post injection, when the experiment was terminated. Abdomens were sampled as described above.
RNA isolation, cDNA Synthesis, and Quantitative PCR (qPCR) on experimentally infected queens
Each abdomen was transferred into a 2 mL Ruptor homogenizing tube containing 5 ceramic beads. The samples were then homogenized using the automated Bead Ruptor Elite (Omni International, Kennesaw, GA, USA) at a speed of 5 m per second (5 m/s) for 20 s. Followed by homogenization, total RNA was extracted using an established TRIzol™ (Invitrogen, Carlsbad, CA, USA) protocol. The concentration and purity of the extracted RNA samples were measured using a Nanodrop OneC Microvolume UV–Vis Spectrophotometer (Thermo Fisher Scientific, MA, USA). The total RNA concentration was adjusted to 20 ng/µL in molecular grade water (Fisher Scientific, Fair Lawn, NJ, USA). Then, a two-step quantitative qPCR assay was carried out to quantify the target genes. For each sample, cDNA was synthesized using the High-Capacity cDNA Reverse-Transcription Kit (Applied Biosystems, Foster City, CA, USA). Ten microliters of the RNA template (total, 200 ng) were added to 10 µL of the provided cDNA master mix, followed by an incubation period as recommended by the manufacturer; 10 min at 25 °C, 120 min at 37 °C, and 5 min at 85 °C. The resulting cDNA solution then diluted tenfold in molecular grade water to serve as template in subsequent qPCR. The qPCR was conducted in duplicate using 384-well plates on a QuantStudio™ 6 cycler (Thermo Fisher Scientific). The reactions were performed using unlabeled primers and SYBR Green DNA binding dye (Applied Biosystems) with a volume of 12 µL with the final primer concentrations of 0.4 µM. We added RNase-free water as template for a No Target Control (NTC) and a No Reverse Transcriptase (NRT) control as an additional negative control68. The thermal cycling conditions were 10 min at 95 °C, followed by 40 cycles consisting of a denaturing stage at 95 °C for 15 s and an annealing/extension stage at 60 °C for 1 min. This procedure was followed by a final melt-curve dissociation analysis to confirm the specificity of the products. For IAPV, the Cq values were determined at the same fluorescence threshold (0.05) for all plates, and a Cq value of 35 or lower was recorded as positive amplification. For three genes of interest and three housekeeping genes we collected Cq values based on the default threshold as determined by the QuantStudio™ 6 for each target gene. Fluoresence measurements were taken at the end of each cycle.
We quantified the IAPV intensity and the expression level of three genes of interest including vitellogenin, protein lethal(2)essential for life-like, heat shock protein 70 cognate 4 (see Supplementary Table S5 for primer sequences). Three housekeeping genes including actin, glyceraldehyde 3-phosphate dehydrogenase (GapDH) and ribosomal protein S5 (RPS5) were also amplified as internal control and for relative quantification. The primers used in this experiment have previously been validated to detect the intended targets and are commonly used in honey bees69,70. We quantified immune-gene expression for each sample using relative quantification71. We calculated geometric mean of the Cq value from three reference genes (RPS5, actin, and GapDH) to confirm amplification. We subtracted the Cq value of geometric mean from target gene for each sample (∆Cq = Cq(gene of interest) − Cq(GMean of reference genes)). To calculate the ∆∆Cq for each target gene, we subtracted the average of the ∆Cq values across the control samples at each age from the ∆Cq for each target gene. The fold-change in gene expression was then calculated using the 2−(∆∆Cq) method. Pairwise comparisons of gene expression were evaluated using the wilcox.test function in the ggpubr package (v0.4.0). Data are plotted in the form of log10(fold change in gene expression) for clarity.
All data underlying the figures in this manuscript are supplied as supplementary information. The previously published (spermathecal fluid) raw data are publicly available on the MassIVE archive (www.massive.ucsd.edu, accession MSV000085428). The ovary mass spectrometry data are available on the MassIVE archive (www.massive.ucsd.edu, accession MSV000087150). Source code underlying figures and data analysis are available freely upon request.
McDermott, A. News feature: To understand the plight of insects, entomologists look to the past. Proc. Natl. Acad. Sci. U. S. A. https://doi.org/10.1073/pnas.2018499117 (2021).
Hallmann, C. A. et al. More than 75 percent decline over 27 years in total flying insect biomass in protected areas. PLoS ONE 12, e0185809. https://doi.org/10.1371/journal.pone.0185809 (2017).
Møller, A. P. Parallel declines in abundance of insects and insectivorous birds in Denmark over 22 years. Ecol. Evol. 9, 6581–6587. https://doi.org/10.1002/ece3.5236 (2019).
Bell, J. R., Blumgart, D. & Shortall, C. R. Are insects declining and at what rate? An analysis of standardised, systematic catches of aphid and moth abundances across Great Britain. Insect. Conserv. Divers. 13, 115–126. https://doi.org/10.1111/icad.12412 (2020).
Wagner, D. L. Insect declines in the anthropocene. Annu. Rev. Entomol. 65, 457–480. https://doi.org/10.1146/annurev-ento-011019-025151 (2020).
Goulson, D. The insect apocalypse, and why it matters. Curr. Biol. 29, R967–R971. https://doi.org/10.1016/j.cub.2019.06.069 (2019).
Crossley, M. S. et al. No net insect abundance and diversity declines across US Long Term Ecological Research sites. Nat. Ecol. Evol. 4, 1368–1376. https://doi.org/10.1038/s41559-020-1269-4 (2020).
van Klink, R. et al. Meta-analysis reveals declines in terrestrial but increases in freshwater insect abundances. Science 368, 417–420. https://doi.org/10.1126/science.aax9931 (2020).
Potts, S. G. et al. Global pollinator declines: Trends, impacts and drivers. Trends Ecol. Evol. 25, 345–353. https://doi.org/10.1016/j.tree.2010.01.007 (2010).
Harris, J. E., Rodenhouse, N. L. & Holmes, R. T. Decline in beetle abundance and diversity in an intact temperate forest linked to climate warming. Biol. Conserv. 240, 108219 (2019).
Halsch, C. A. et al. Insects and recent climate change. Proc. Natl. Acad. Sci. U. S. A. https://doi.org/10.1073/pnas.2002543117 (2021).
Soroye, P., Newbold, T. & Kerr, J. Climate change contributes to widespread declines among bumble bees across continents. Science 367, 685–688 (2020).
Sales, K. et al. Experimental heatwaves compromise sperm function and cause transgenerational damage in a model insect. Nat. Commun. 9, 4771. https://doi.org/10.1038/s41467-018-07273-z (2018).
Nanetti, A., Bortolotti, L. & Cilia, G. Pathogens spillover from honey bees to other arthropods. Pathogens https://doi.org/10.3390/pathogens10081044 (2021).
Salehipour-Shirazi, G., Ferguson, L. V. & Sinclair, B. J. Does cold activate the Drosophila melanogaster immune system?. J. Insect Physiol. 96, 29–34 (2017).
Ferguson, L. V. Thermal biology of insect immunity and host–microbe interactions. (2017).
Rowland, B. W., Rushton, S. P., Shirley, M. D. F., Brown, M. A. & Budge, G. E. Identifying the climatic drivers of honey bee disease in England and Wales. Sci. Rep. 11, 21953. https://doi.org/10.1038/s41598-021-01495-w (2021).
Murray, E. A. et al. Viral transmission in honey bees and native bees, supported by a global black queen cell virus phylogeny. Environ. Microbiol. 21, 972–983. https://doi.org/10.1111/1462-2920.14501 (2019).
McMahon, D. P. et al. A sting in the spit: widespread cross-infection of multiple RNA viruses across wild and managed bees. J. Anim. Ecol. 84, 615–624. https://doi.org/10.1111/1365-2656.12345 (2015).
Fürst, M. A., McMahon, D. P., Osborne, J. L., Paxton, R. J. & Brown, M. J. Disease associations between honeybees and bumblebees as a threat to wild pollinators. Nature 506, 364–366. https://doi.org/10.1038/nature12977 (2014).
Alger, S. A., Burnham, P. A., Boncristiani, H. F. & Brody, A. K. RNA virus spillover from managed honeybees (Apis mellifera) to wild bumblebees (Bombus spp.). PLoS ONE 14, e0217822. https://doi.org/10.1371/journal.pone.0217822 (2019).
Purkiss, T. & Lach, L. Pathogen spillover from Apis mellifera to a stingless bee. Proc. Biol. Sci. 286, 20191071. https://doi.org/10.1098/rspb.2019.1071 (2019).
Colla, S. R., Otterstatter, M. C., Gegear, R. J. & Thomson, J. D. Plight of the bumble bee: Pathogen spillover from commercial to wild populations. Biol. Cons. 129, 461–467. https://doi.org/10.1016/j.biocon.2005.11.013 (2006).
Pritchard, Z. A. et al. Do viruses from managed honey bees (Hymenoptera: Apidae) endanger wild bees in native prairies?. Environ. Entomol. https://doi.org/10.1093/ee/nvaa181 (2021).
Burnham, P. A. et al. Flowers as dirty doorknobs: Deformed wing virus transmitted between Apis mellifera and Bombus impatiens through shared flowers. J. Appl. Ecol. 58, 2065–2074 (2021).
Meeus, I., de Miranda, J. R., de Graaf, D. C., Wäckers, F. & Smagghe, G. Effect of oral infection with Kashmir bee virus and Israeli acute paralysis virus on bumblebee (Bombus terrestris) reproductive success. J. Invertebr. Pathol. 121, 64–69 (2014).
Gauthier, L. et al. Viruses associated with ovarian degeneration in Apis mellifera L. queens. PLoS ONE 6, e16217. https://doi.org/10.1371/journal.pone.0016217 (2011).
Schwenke, R. A., Lazzaro, B. P. & Wolfner, M. F. Reproduction–immunity trade-offs in insects. Annu. Rev. Entomol. 61, 239–256 (2016).
Nystrand, M. & Dowling, D. K. Dose-dependent effects of an immune challenge at both ultimate and proximate levels in Drosophila melanogaster. J. Evol. Biol. 27, 876–888. https://doi.org/10.1111/jeb.12364 (2014).
Ahmed, A., Baggott, S., Maingon, R. & Hurd, H. The costs of mounting an immune response are reflected in the reproductive fitness of the mosquito Anopheles gambiae. Oikos 97, 371–377 (2002).
Stahlschmidt, Z. R., Rollinson, N., Acker, M. & Adamo, S. A. Are all eggs created equal? Food availability and the fitness trade-off between reproduction and immunity. Funct. Ecol. 27, 800–806 (2013).
Myers, J. H. & Cory, J. S. Ecology and evolution of pathogens in natural populations of Lepidoptera. Evol. Appl. 9, 231–247. https://doi.org/10.1111/eva.12328 (2016).
McNamara, K. B., van Lieshout, E. & Simmons, L. W. Females suffer a reduction in the viability of stored sperm following an immune challenge. J. Evol. Biol. 27, 133–140. https://doi.org/10.1111/jeb.12278 (2014).
Chérasse, S. & Aron, S. Impact of immune activation on stored sperm viability in ant queens. Proc. Biol. Sci. 285, 20182248. https://doi.org/10.1098/rspb.2018.2248 (2018).
McAfee, A., Chapman, A., Pettis, J. S., Foster, L. J. & Tarpy, D. R. Trade-offs between sperm viability and immune protein expression in honey bee queens (Apis mellifera). Commun. Biol. 4, 48. https://doi.org/10.1038/s42003-020-01586-w (2021).
Rueppell, O., Aumer, D. & Moritz, R. F. Ties between ageing plasticity and reproductive physiology in honey bees (Apis mellifera) reveal a positive relation between fecundity and longevity as consequence of advanced social evolution. Curr. Opin. Insect Sci. 16, 64–68. https://doi.org/10.1016/j.cois.2016.05.009 (2016).
Winston, M. L. The Biology of the Honey Bee (Harvard University Press, 1991).
Baer, B., Eubel, H., Taylor, N. L., O’Toole, N. & Millar, A. H. Insights into female sperm storage from the spermathecal fluid proteome of the honeybee Apis mellifera. Genome Biol. 10, R67. https://doi.org/10.1186/gb-2009-10-6-r67 (2009).
Paynter, E. et al. Insights into the molecular basis of long-term storage and survival of sperm in the honeybee (Apis mellifera). Sci. Rep. 7, 40236. https://doi.org/10.1038/srep40236 (2017).
Radhakrishnan, P. & Fedorka, K. M. Immune activation decreases sperm viability in both sexes and influences female sperm storage. Proc. Biol. Sci. 279, 3577–3583. https://doi.org/10.1098/rspb.2012.0654 (2012).
McAfee, A. et al. Candidate stress biomarkers for queen failure diagnostics. BMC Genom. 21, 571. https://doi.org/10.1186/s12864-020-06992-2 (2020).
McAfee, A. et al. Vulnerability of honey bee queens to heat-induced loss of fertility. Nat. Sustain. 3, 1–10 (2020).
Amiri, E., Strand, M. K., Tarpy, D. R. & Rueppell, O. Honey bee queens and virus infections. Viruses 12, 322 (2020).
Amiri, E., Strand, M. K., Rueppell, O. & Tarpy, D. R. Queen quality and the impact of honey bee diseases on queen health: Potential for interactions between two major threats to colony health. Insects 8, 48 (2017).
Simeunovic, P. et al. Nosema ceranae and queen age influence the reproduction and productivity of the honey bee colony. J. Apic. Res. 53, 545–554 (2014).
Al Naggar, Y. & Paxton, R. J. Mode of transmission determines the virulence of black queen cell virus in adult honey bees, posing a future threat to bees and apiculture. Viruses https://doi.org/10.3390/v12050535 (2020).
Amiri, E., Herman, J. J., Strand, M. K., Tarpy, D. R. & Rueppell, O. Egg transcriptome profile responds to maternal virus infection in honey bees, Apis mellifera. Infect. Genet. Evol. 85, 104558. https://doi.org/10.1016/j.meegid.2020.104558 (2020).
Cobey, S. W., Tarpy, D. R. & Woyke, J. Standard methods for instrumental insemination of Apis mellifera queens. J. Apic. Res. 52, 1–18 (2013).
Salmela, H., Amdam, G. V. & Freitak, D. Transfer of immunity from mother to offspring is mediated via egg-yolk protein vitellogenin. PLoS Pathog. 11, e1005015. https://doi.org/10.1371/journal.ppat.1005015 (2015).
Harwood, G., Amdam, G. & Freitak, D. The role of Vitellogenin in the transfer of immune elicitors from gut to hypopharyngeal glands in honey bees (Apis mellifera). J. Insect Physiol. 112, 90–100. https://doi.org/10.1016/j.jinsphys.2018.12.006 (2019).
Thuaud, F., Ribeiro, N., Nebigil, C. G. & Désaubry, L. Prohibitin ligands in cell death and survival: Mode of action and therapeutic potential. Chem. Biol. 20, 316–331. https://doi.org/10.1016/j.chembiol.2013.02.006 (2013).
Thompson, W. E. et al. Regulation of prohibitin expression during follicular development and atresia in the mammalian ovary. Biol. Reprod. 71, 282–290. https://doi.org/10.1095/biolreprod.103.024125 (2004).
Kuadkitkan, A., Wikan, N., Fongsaran, C. & Smith, D. R. Identification and characterization of prohibitin as a receptor protein mediating DENV-2 entry into insect cells. Virology 406, 149–161. https://doi.org/10.1016/j.virol.2010.07.015 (2010).
Merkling, S. H. et al. The heat shock response restricts virus infection in Drosophila. Sci. Rep. 5, 12758. https://doi.org/10.1038/srep12758 (2015).
McMenamin, A. J., Daughenbaugh, K. F. & Flenniken, M. L. The heat shock response in the western honey bee (Apis mellifera) is antiviral. Viruses https://doi.org/10.3390/v12020245 (2020).
Adamo, S. A., Roberts, J. L., Easy, R. H. & Ross, N. W. Competition between immune function and lipid transport for the protein apolipophorin III leads to stress-induced immunosuppression in crickets. J. Exp. Biol. 211, 531–538. https://doi.org/10.1242/jeb.013136 (2008).
Weers, P. M. & Ryan, R. O. Apolipophorin III: Role model apolipoprotein. Insect Biochem. Mol. Biol. 36, 231–240. https://doi.org/10.1016/j.ibmb.2006.01.001 (2006).
Arrese, E. L. & Soulages, J. L. Insect fat body: Energy, metabolism, and regulation. Annu. Rev. Entomol. 55, 207–225 (2010).
Brutscher, L. M., Daughenbaugh, K. F. & Flenniken, M. L. Virus and dsRNA-triggered transcriptional responses reveal key components of honey bee antiviral defense. Sci. Rep. 7, 6448. https://doi.org/10.1038/s41598-017-06623-z (2017).
Amiri, E., Meixner, M. D. & Kryger, P. Deformed wing virus can be transmitted during natural mating in honey bees and infect the queens. Sci. Rep. 6, 33065. https://doi.org/10.1038/srep33065 (2016).
Adamo, S. A. Animals have a Plan B: How insects deal with the dual challenge of predators and pathogens. J. Comp. Physiol. B 190, 381–390. https://doi.org/10.1007/s00360-020-01282-5 (2020).
Collins, A. & Donoghue, A. Viability assessment of honey bee, Apis mellifera, sperm using dual fluorescent staining. Theriogenology 51, 1513–1523 (1999).
Lee, K. V., Goblirsch, M., McDermott, E., Tarpy, D. R. & Spivak, M. Is the brood pattern within a honey bee colony a reliable indicator of queen quality?. Insects 10, 12 (2019).
Vandesompele, J. et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3, RESEARCH0034. https://doi.org/10.1186/gb-2002-3-7-research0034 (2002).
Rappsilber, J., Ishihama, Y. & Mann, M. Stop and go extraction tips for matrix-assisted laser desorption/ionization, nanoelectrospray, and LC/MS sample pretreatment in proteomics. Anal. Chem. 75, 663–670 (2003).
Ritchie, M. E. et al. limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 43, e47–e47 (2015).
Büchler, R. et al. Standard methods for rearing and selection of Apis mellifera queens. J. Apic. Res. 52, 1–30 (2013).
Bustin, S. A. et al. The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 55, 611–622. https://doi.org/10.1373/clinchem.2008.112797 (2009).
Jeon, J. H., Moon, K., Kim, Y. & Kim, Y. H. Reference gene selection for qRT-PCR analysis of season- and tissue-specific gene expression profiles in the honey bee Apis mellifera. Sci. Rep. 10, 13935. https://doi.org/10.1038/s41598-020-70965-4 (2020).
Deng, Y., Zhao, H., Yang, S., Zhang, L. & Hou, C. Screening and validation of reference genes for RT-qPCR under different honey bee viral infections and dsRNA treatment. Front. Microbiol. 11, 1715. https://doi.org/10.3389/fmicb.2020.01715 (2020).
Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402–408. https://doi.org/10.1006/meth.2001.1262 (2001).
Honey bee research in LJF’s group is supported by an NSERC Discovery Grant. Mass spectrometry infrastructure is supported by Genome Canada and Genome BC (264PRO) and computational infrastructure is supported by a Compute Canada Resource Allocation to LJF. AM was supported by an NSERC Postdoctoral Fellowship and a L’Oreal-UNESCO for Women in Science Research Excellence fellowship. A Project Apis m. Grant to AM and LJF helped fund this research, as well as a Boone–Hodgeson–Wilkinson Trust Fund Grant to AM and LJF. AC was supported by a Christi Heintz Memorial Award. A part of this research was performed while EA held an NRC Research Associateship award and supported by the US Army Research Office (Grant # W911NF1520045) and US Department of Agriculture-APHIS (AP20PPQS&T00C014). We would like to acknowledge Hives for Humanity, Robyn Underwood, and numerous beekeepers in the BC Bee Breeders’ Association for their contribution to our queen surveys.
The authors declare no competing interests.
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Chapman, A., Amiri, E., Han, B. et al. Fertility costs of cryptic viral infections in a model social insect. Sci Rep 12, 15857 (2022). https://doi.org/10.1038/s41598-022-20330-4