Abstract
Sparganium longifolium was reported as a hybrid between S. emersum and S. gramineum based on its intermediate type or the common characteristics of its parent species. Its hybrid origin needs to be confirmed using molecular technology. We investigated the origin of S. longifolium based on 10 populations of S. emersum, S. gramineum and S. longifolium from five lakes in European Russia, using sequences of six nuclear loci and one chloroplast DNA fragment. Haplotype network, principal coordinate analysis and genetic clustering based on data of nuclear loci confirmed that S. longifolium is the hybrid between S. emersum and S. gramineum. We found that the natural hybridization between S. emersum and S. gramineum is bidirectional but asymmetrical, and the latter mainly acts as maternal species. We also found that all samples of S. longifolium were F1 generations, and thus hypothesized that S. emersum and S. gramineum could likely maintain their species boundary through the post-zygote reproductive isolation mechanism of F1 generation sterility.
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Introduction
Sparganium L. (Typhaceae) is an ecologically important group of aquatic plants, comprising about 14 species, widely distributed in the temperate and cold regions of the northern hemisphere1,2. Natural hybridization between Sparganium species is common. Cook and Nicolls reviewed previous studies and listed different interspecific hybrids in Sparganium. All these studies identified hybrids using morphological characteristics. Due to phenotypic plasticity and subtle morphological differences, however, hybrids are often difficult to distinguish from their parents3. Therefore, molecular identification is an essential method for the study of natural hybridization in Sparganium. Recently, the presence of hybrids, such as S. angustifolium Michx. × S. emersum Rehmann, S. fallax Graebn. × S. japonicum Rothert, S. acaule Rydb. × S. fluctuans B.L.Rob. and S. glomeratum Laest. ex Beurl. × S. gramineum Georgi was verified based on DNA sequences in two phylogenetic studies3,4.
Sparganium longifolium Turcz. was reported as a hybrid between S. emersum and S. gramineum based on their morphological characteristics1. This hybrid occurs commonly in regions where both parents grow together and often occupies habitats where S. gramineum is absent due to its high adaptability under eutrophic conditions1. The hybrid origin of S. longifolium was emphasized by5 based on their detailed biomorphological investigation. The hybrid was considered fertile1 and backcrossing with its parent species was used to explain the phenomenon that some populations of S. longifolium were rich in terate forms5. So far, only one case study at the molecular level has been conducted for S. longifolium. Belyakov et al. sequenced the internal transcribed spacer and found similar and identical ribotypes in S. emersum, S. longifolium, S. gramineum and S. hyperboreum, which did not provide directly molecular evidence to clarify the origin of S. longifolium6. Further molecular studies are still necessary to confirm the hybrid origin of S. longifolium. In addition, the direction of hybridization is unknown. If a bidirectional hybridization exists, two distinct life forms of S. longifolium, emergent and floating-leaved, which are similar to S. emersum and S. gramineum respectively, are likely correlated to the direction of hybridization. All these hypotheses need to be verified using molecular technology.
In this study, we collected samples of S. emersum, S. gramineum and S. longifolium from five lakes in European Russia, using sequences of six nuclear loci and one chloroplast DNA fragment to (1) test whether S. longifolium is the hybrid between S. emersum and S. gramineum, and (2) detect the direction of hybridization. The study will deepen our understanding of interspecific hybridization in Sparganium.
Results
Sequence variations
Sequences of the six nuclear loci were obtained from 85, 83, 62, 85, 84 and 86 individuals, respectively. Their aligned lengths were 579, 492, 501, 465, 326 and 413 bp with 4, 14, 4, 10, 7 and 6 variable sites, respectively (Supplementary Table S1). The numbers of haplotypes at the six nuclear loci were 3, 13, 2, 2, 7 and 3, respectively. The haplotype networks of the six nuclear loci showed the same pattern: haplotypes of S. emersum and S. gramineum separated well and formed two clades, while all individuals of S. longifolium were heterozygous and consisted of two alleles from different haplotype clades (Fig. 1). Of the six nuclear loci, only at the Tran57 locus S. longifolium had private haplotypes (Fig. 1).
Sequences of trnH-psbA were obtained from 82 individuals, including 15 of S. emersum, 20 of S. gramineum and 47 of S. longifolium. The aligned length was 670 bp with four substitutions and two 4-bp indels. Sequences of all samples collapsed into two haplotypes H1 and H2 corresponding to S. emersum and S. gramineum, respectively (Fig. 1g). In S. longifolium, nine individuals from VL and PYL populations shared H1 with S. emersum and 38 individuals from PL, PYL, ZL and SL populations shared H2 with S. gramineum (Fig. 1g).
Genetic grouping
The PCoA analysis revealed that all samples were divided into three groups on principal coordinate 1, which explained 38.73% of the total variation. The three groups corresponded to S. emersum, S. gramineum and S. longifolium, respectively, and the hybrid group was located between two parent groups (Fig. 2a). STRUCTURE analysis suggested K = 2 as the optimal number of clusters based on the value of ΔK (Supplementary Fig. S1) and inferred two genetic clusters that consisted of S. emersum and S. gramineum respectively and genetic admixture for all samples of S. longifolium with intermediate admixture coefficient (0.3863–0.5279, Fig. 2b).
Discussion
The haplotype networks, PCoA analysis and STRUCTURE analysis based on the six nuclear loci confirm that S. longifolium is a hybrid between S. emersum and S. gramineum, providing molecular support for previous morphological analyses5. Furthermore, all individuals with intermediate admixture coefficient (Fig. 2b) and private haplotypes only present in one out of six nuclear loci (Fig. 1) suggest that S. longifolium is most likely a F1 hybrid. We thus hypothesized that S. emersum and S. gramineum could likely maintain their species boundary through the post-zygote reproductive isolation mechanism of F1 generation sterility. This hypothesis is possible based on the observations from hybrids in European Russia. The pollen viability was checked in S. longifolium samples from Vysokovskoe Lake and Sabro Lake, and the vast majority of checked pollens were sterile5. In addition, flowering plants of S. longifolium often do not form seeds, or the seeds are puny and significantly inferior to normal seeds in size5. However, the hypothesis is only based on our limited sampling, which is contrary to the conclusion inferred from morphological characteristics that it is fertile and may backcross with parental species1. Further studies with extensive sampling are necessary to test our hypothesis.
The chloroplast DNA fragment trnH-psbA was used to infer the direction of hybridization between S. emersum and S. gramineum because chloroplast DNA is maternal inheritance in Sparganium3,4. The hybrid S. longifolium shared haplotypes with S. emersum and S. gramineum simultaneously (Fig. 1). This finding clearly indicates that bidirectional hybridization exists between S. emersum and S. gramineum. At the same time, the different frequency of these two haplotypes in the hybrid (H1, 19.1% vs. H2, 80.9%) means that the direction of hybridization is asymmetric. A variety of factors can lead to asymmetry in natural hybridization, such as flowering time, preference of pollinators, quality and quantity of pollen, cross incompatibility and the abundance of parent species7,8. Rare species usually act as maternal species relative to abundant species9,10. S. gramineum is confined to oligotrophic lakes and its abundance is obviously lower than that of S. emersum1,11. The relatively scarcity combined with the ecology of S. gramineum make it more often act as maternal species when hybridizing with S. emersum.
As described by5, the morphological diversification of S. longifolium was also observed in this study. For example, individuals of S. longifolium with emergent and floating-leaved life forms occur concurrently in Zaozer’ye Lake (Supplementary Fig. S2). However, all individuals had the same haplotype H2 as S. gramineum (Fig. 1), suggesting that the direction of hybridization do not determine life form of S. longifolium. In addition, all individuals of S. longifolium sampled here are likely F1 hybrid. Their variable phenotypes could not be associated with traits segregation due to F2 generation or backcross. Detailed ecological investigation combining with research at the genomic level are essential to find out the potential factors leading to morphological diversification of S. longifolium.
Here, using sequences of six nuclear loci and one chloroplast DNA fragment, we confirmed that S. longifolium is the hybrid between S. emersum and S. gramineum. The natural hybridization between S. emersum and S. gramineum is bidirectional but the latter mainly acts as maternal species. We also found that all samples of S. longifolium were F1 generations, indicating that S. emersum and S. gramineum could maintain their species boundary through the post-zygote reproductive isolation mechanism of F1 generation sterility.
Methods
Sample collection and DNA extraction
A total of 93 individuals from 10 populations of S. emersum, S. gramineum and S. longifolium were collected from five lakes in European Russia (Table 1). Individuals of each population were collected randomly at intervals of at least 10 m. The collection of plant materials was approved by Papanin Institute for Biology of Inland Water (IBIW), Russian Academy of Sciences. Voucher specimens were kept in the herbarium of IBIW and identified by Dr. Eugeny A. Belyakov (IBIW). Fresh leaves were sampled and dried in silica gel for subsequent DNA extraction. Total genomic DNA was extracted using the DNA Secure Plant Kit (Tiangen Biotech, Beijing, China) following the manufacturer’s protocol.
Amplification, sequencing and cloning
We sequenced one chloroplast DNA fragment trnH-psbA12 and six nuclear loci developed from transcriptome sequences of S. fallax (Supplementary Table S1). PCR reactions, sequencing and cloning were performed following13. All individuals of S. longifolium were heterozygous at the six nuclear loci with multi-point mutations or insertions/deletions, and their alleles were obtained by cloning. Sequence data were aligned using MAFFT v7.3.114.
Data analyses
Haplotypes of each locus were identified using DNASP v5.015. The obtained haplotypes were deposited in GenBank (see Supplementary Table S1 for accession numbers). A median-joining network16 to interpret relationships among haplotypes of each locus was generated using NETWORK v4.0 (http://www.fluxus-engineering.com). The following analyses were performed based on the dataset of six nuclear loci. The genetic lineage proportion of each individual was identified using a Bayesian clustering method implemented in STRUCTURE v2.417. We performed 10 replicate runs with a burn-in period of 20,000 iterations and 100,000 Markov Chain Monte Carlo (MCMC) iterations under the admixture model at the number of clusters from one to eight. Principal coordinate analysis (PCoA) implemented in GenALEx v6.518, was also used to examine the genetic clusters of all individuals.
All methods were performed in accordance with the relevant guidelines and regulations.
Data availability
The data that support the findings of this study are available in the GenBank with accession numbers ON015932-ON015962, [https://www.ncbi.nlm.nih.gov/].
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Acknowledgements
This research was funded by the “Fundamental Research Funds for the Central Universities (No. 2042020kf1025)” and Russian Foundation for Basic Research (RFBR) “Molecular phylogeny, adaptive potential and features of the morphological evolution of some vascular plants in the development of the aquatic environment (No. 18-34-00257)”. We thank Haihao Yu for his assistance in field collection.
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X.X., E.A.B. and A.G.L. designed the research; X.X., A.G.L. and E.A.B. carried out the field collections; Y.Y., F.L. and W.Y. carried out the experiments and performed the data analysis; X.X., E.A.B. and A.G.L. wrote and revised the manuscript.
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Yu, Y., Li, F., Belyakov, E.A. et al. Molecular confirmation of the hybrid origin of Sparganium longifolium (Typhaceae). Sci Rep 12, 7279 (2022). https://doi.org/10.1038/s41598-022-11222-8
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DOI: https://doi.org/10.1038/s41598-022-11222-8
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