One of the most common responses of marine ectotherms to rapid warming is a reduction in body size, but the underlying reasons are unclear. Body size reductions have been documented alongside rapid warming events in the fossil record, such as across the Pliensbachian-Toarcian boundary (PToB) event (~ 183 Mya). As individuals grow, parallel changes in morphology can indicate details of their ecological response to environmental crises, such as changes in resource acquisition, which may anticipate future climate impacts. Here we show that the morphological growth of a marine predator belemnite species (extinct coleoid cephalopods) changed significantly over the PToB warming event. Increasing robustness at different ontogenetic stages likely results from indirect consequences of warming, like resource scarcity or hypercalcification, pointing toward varying ecological tolerances among species. The results of this study stress the importance of taking life history into account as well as phylogeny when studying impacts of environmental stressors on marine organisms.
The most common responses of recent and fossil marine invertebrates to global warming are extinctions1, changes in geographical distribution2, phenology3,4, and decreases in body size5,6,7,8,9,10. However, many biological mechanisms may underlie body size reductions8,10,11,12,13. Generally, individuals in colder environments grow more slowly but become larger as adults14. This widespread pattern is embodied by two well-established hypotheses: Bergmann's rule, which describes the negative association between temperature and body size in natural environments15, and the temperature-size rule (TSR)16, which describes physiological reaction norms relating temperature to body size in laboratory experiments8,11,17,18. These hypotheses predict that organisms should grow to be larger in colder environments, because growth efficiency decreases with increasing environmental temperature14. Whether through metabolic processes19,20 or reduced resource availability, exposure to higher temperatures and accompanying factors like increased pCO2 during early development have been shown to lead to altered growth processes21,22,23. Possible outcomes include abnormal growth during the juvenile life stage, or precocious maturity13,24, which might lead to paedomorphosis, a heterochronic change marked by retention of juvenile features in adult organisms5. While a decrease in body size has been reported from various past hyperthermal events25,26,27,28,29,30,31,32,33, morphological changes of different life stages are not commonly studied34 although they are crucial for understanding long-term impacts on life history and their ecological implications.
Here we tackle this issue by focusing on one of the most severe extinction crises of the Jurassic: the Pliensbachian-Toarcian crisis. This event was a global, multi-phased, hyperthermal event triggered by volcanic episodes in the Karoo-Ferrar Large Igneous Province35,36,37,38, which led to the extinction of around 15–20% of marine species39. This disturbance was characterized by an early abrupt warming of at least 5° C at the Pliensbachian-Toarcian boundary event (PToB)40,41,42,43, which is considered a precursor crisis to the early Toarcian warming and oceanic anoxic event (T-OAE)44,45.
The Peniche GSSP section (Portugal) offers an excellent geochemical and fossil record of the PToB crisis (Fig. 1). Trace fossils are present throughout the section, indicating consistently oxygenated bottom waters46. Sedimentological and geochemical proxies show the rapid warming event40,41,42,43 and sea-level transgression at the PToB45 with evidence for ocean acidification42, increased weathering41, collapsed productivity and resource scarcity40,47, which was followed in the early Toarcian by a second, more severe environmental crisis (the T-OAE) (Fig. 1). The temporary disappearance of macroinvertebrates like belemnites (Fig. 1), additionally indicates harsh conditions during the T-OAE48. Belemnites are among the organisms that suffered considerably during this crisis48,49,50,51,52. Their abundance in the fossil record48,53,54,55, and rapid response to environmental changes, makes these predators suitable for studying responses to past climate changes.
A significant size decrease in adults of the dominant belemnite species was evidenced across the PToB in Peniche48, while the extinction of forms with slender belemnite rostra but consistent diversity of robust forms is interpreted as a selective extinction against deep-water dwelling slender forms which were sensitive to decreased oxygen concentrations51.
Here we use 3D landmark analysis56 to quantify morphological changes of belemnites across the PToB crisis in Peniche. Basing our hypotheses on prior studies, we investigate, whether the size reduction across the boundary was accompanied by allometric changes and thus affected certain life stages disproportionally. Firstly, we expect the largest shift in morphospace (see results and methods) across the PToB as an increase in robustness48,51. If this was driven by accelerated growth, we expect a within-species morphological change driven by paedomorphosis of the best represented species48. We test for morphological changes on the (1) assemblage and (2) species level and (3) by life stage. Furthermore, we expect these changes to be correlated with increased temperature, rather than with severe shifts in depositional environments.
Belemnite morphospace structuration
The consensus shape produced through the Generalized Procrustes Analysis (GPA) is a ventral to dorsal profile through an almost cylindrical belemnite rostrum of medium robustness. The alveolar walls at the reference point on transversal sections are of average thickness and the protoconch is located close to the centre of the rostrum with almost equal distance to the dorsal and ventral sides57. The first axis of the Principal Components Analysis (PCA) represents the ratio of width to length (robustness) of the cross-section and accounts for 82% of morphological variation in the data. PC2 explains 7% of the variance and the morphological variation is only nuanced, contrasting thin from thick alveolar walls in the transversal section representing another aspect of rostrum robustness. Based on the percentage of explained variance, we use PC1 as our main morphology proxy, and use ‘PC1’ and ‘robustness’ interchangeably.
The studied species occupy different regions of the morphospace (Fig. 2, supplementary table S1). More slender species include Bairstowius amaliae, Catateuthis longiforma, Acrocoelites sp. and indeterminable Hastitidae specimens. Out of these, the robustness of C. longiforma and B. amaliae differs significantly (p = 0.014). Passaloteuthis milleri and Passaloteuthis bisulcata show a wide range in terms of robustness from moderately slender to very robust shapes. Significant differences between both of these more robust species and B. amaliae are found (P. milleri, p < 0.001, P. bisulcata, p < 0.001). Additionally, the robustness of C. longiforma is significantly lower than both, P. milleri and P. bisulcata, too (P. milleri, p = 0.003, P. bisulcata, p < 0.001). Parapassaloteuthis aff. zieteni is, on average, the most robust species, differing significantly from all other species (P. bisulcata, p = 0.006, P. milleri, p < 0.001, B. amaliae, p < 0.001, C. longiforma, p < 0.001) (supplementary table S1).
Morphology through time
The full assemblage shows both a small but significant size decrease across the Pliensbachian-Toarcian boundary (Hedges’ g = − 0.022, p = 0.018), as well as a large, significant size increase from the Dactylioceras semicelatum to the Elegantuliceras elegantulum subzone after the T-OAE (Hedges’ g = − 6.302, p = 0.006), in which only two specimens of the species Acrocoelites sp. appear after a comprehensive loss of belemnite abundance and diversity48,58. The body size pattern of the full assemblage is not accompanied by any significant changes in robustness (Fig. 1, supplementary table S3).
The bootstrapping procedure (see methods) shows that the change in species abundances through the subzones is within the realm of random sampling, with the only exception being the Elegantulum subzone (supplementary Fig. S2). Because this sample is from after the T-OAE and the changes in community composition across the boundary appear to be random, we reject the hypothesis that community composition markedly influenced the morphological pattern across the PToB.
For species-level analyses, we focus on P. bisulcata and C. longiforma, which dominate the assemblages across the boundary and are reasonably represented in our morphological subset. Catateuthis longiforma, and to a lesser extent P. bisulcata have been shown to decrease in body size in response to the PToB event48. While the sample of P. bisulcata in our dataset does not change in size (supplementary table S2), C. longiforma shrinks significantly across the boundary (p < 0.001) and increases in body size again in the following subzone (p = 0.015) (Fig. 1, supplementary table S2). As assumed (see methods), both species become more robust across the boundary (P. bisulcata: Hedge’s g = − 1.054, p = 0.043, C. longiforma: Hedge’s g = − 0.844, p = 0.02) (Fig. 1, supplementary table S3). After this initial increase in robustness, C. longiforma returns to a slenderer shape in the Dactylioceras semicelatum subzone (Hedges’ g = 0.229), while P. bisulcata becomes even more robust (Hedges’ g = − 1.165) (Fig. 1, supplementary table S3). However, these changes from the Dactylioceras mirabile subzone to the Semicelatum subzone are not significant (supplementary table S3).
In both species, smaller specimens are generally more robust (Fig. 3). The slope of robustness at size becomes steeper in higher temperatures in both species (P. bisulcata – warm: slope = − 0.003, adj. R2 = 0.778, p < 0.001, P. bisulcata—cold: slope =− 0.001, adj. R2 = − 0.047, p = 0.954, C. longiforma – warm: slope = − 0.004, adj. R2 = 0.309, p = 0.004, C. longiforma—cold: slope = − 0.002, adj. R2 = 0.458, p < 0.001), meaning that the differences in robustness among life stages are less noticeable in lower temperature settings, while they become more pronounced in high temperature habitats (Fig. 3). The difference in growth trajectories between warm and cold settings is insignificant in C. longiforma (p = 0.138), but significant for P. bisulcata (p < 0.001).
The linear regression model produced significant evidence for developmental stage being a good predictor of morphological change in C. longiforma (p = 0.024) (supplementary table S4). Catateuthis longiforma shows a negative allometric growth during both Late Pliensbachian subzones, defined as background times, with older life stages being more slender than juveniles (Fig. 4). The older life stages (neanics and adults) become more robust across the boundary (Hedges’ g = − 0.904, p = 0.054) (supplementary table S5), making it look like the growth pattern switches to isometric growth during the hyperthermal event (Fig. 4). In P. bisulcata adults basically disappear after the boundary and juveniles become significantly more robust over time (p = 0.005) (Fig. 4). However, this seems to be happening gradually, as differences between consecutive subzones are insignificant (supplementary table S5).
The independent effects of temperature reflected by oxygen isotopes (p = 0.002), seawater pH, reflected by boron isotopes (p = 0.028), and lithology (p = 0.001) on overall morphological variation are significant, out of which lithology plays the biggest role with 1.4% (supplementary Fig. S4, supplementary table S6). However, this cannot explain any changes across the boundary as the lithology variable in our samples stays constant there. When correcting for the influence of lithology to identify correlations between robustness and environmental variables, the null model is the most parsimonious for the full assemblage (supplementary table S7). Therefore, no environmental factor seems to consistently influence the overall robustness pattern. For P. bisulcata the model including boron isotopes as proxy for acidification is the most parsimonious (supplementary table S7) and performs significantly better than the null model (p = 0.014) (supplementary table S8). In C. longiforma all variables together, δ18O, δ13C and δ11B, best explain the robustness pattern through time (supplementary table S7). In this model, all environmental variables have a significant correlation with robustness (δ18O: p = 0.001, δ13C: p = 0.01, δ11B: p = 0.002) (supplementary Table S8). These results do not change drastically, when using palaeoenvironmental proxies (δ18O, δ13C, and Hg/TOC) from different publications38,43,59, except for the significant correlation of the morphological pattern of P. bisulcata with the acidification proxy (δ11B), which is not present in the older geochemical publications (supplementary tables S9 & S10).
Belemnites were likely dominant in epipelagic habitats (0–150 m)55 and fast-swimming nektonic predators60, and consequently must have had a demanding metabolism61. Even though we find no significant change in robustness of the full assemblage across the PToB, the two dominant species (C. longiforma and P. bisulcata) both show a significant increase in robustness at the event, which can be attributed to direct and indirect consequences of the warming of the environment, like changes in continental run-off, ocean acidification and resource sparsity40,41,42,46,62. At the onset of the T-OAE, a shift towards more robustly shaped taxa (often interpreted as more nektobenthic, shallow water inhabitants) was interpreted as an effect of anoxia in deeper-water habitats, inhabited by more slender, pelagic belemnite taxa51,55,61,63,64. However, in our material, the random change in relative species abundances across the boundary would be unlikely to impact the increase in assemblage-scale robustness, which leads us to discard the possibility of bathymetric changes or taphonomic processes having a large impact on the morphological signal. If these processes had been substantial, they would have resulted in preferential preservation of more robust species65,66, or an environmentally triggered shift of assemblage composition, which is not the case in our data across the PToB.
We interpret the change in morphology of adult specimens that occurs parallel to the decrease in size in C. longiforma as an indication for a paedomorphosis by progenesis, i.e. the retention of juvenile morphology in stunted adult specimens5,34,67,68,69,70. A body size decrease in adults of C. longiforma has been observed in the full data set of belemnites in Peniche before48. This conclusion is further supported by a correlation of the morphological pattern with environmental changes in palaeotemperature, seawater pH and carbon cycle and the fast recovery after the crisis. In this species juveniles show a lower sensitivity to climate warming than adults. Differential sensitivities of life stages to climate-related stressors (CRS) have been found in many recent metazoan groups71,72,73,74. It has been argued that particularly very early life stages and spawning adults of marine ectotherms might be particularly susceptible to CRS8,71,75, while larger juvenile and young adults should be more resistant. If juvenile growth and reproduction is hindered, this likely will impact their population success24, which, if happening over larger parts of their geographic range, augments their extinction risk76,77. Catateuthis longiforma appeared to respond to the warming event by developmental changes resulting in smaller and more robust adults, and then recovered in morphology and population structure following the initial warming event. This suggests its changes in life history strategy allowed this species to more successfully cope with the initial warming event, which is reminiscent of observations on modern ectotherms11,13.
Although the sample sizes of the different P. bisulcata ontogenetic stages are too small for sound ecological inferences, juveniles of this species sampled after the PToB appeared to show abnormal robustness patterns. If future morphological studies validated this pattern, this could be related to a change in growth in the early development. Many experimental studies on modern squid show that several climate-related stressors can change the morphology of squid’s internal skeletons24,78,79,80,81. In particular, decreased pH in seawater is associated with abnormal early development of statoliths81 and cuttlebones in decabrachians as well as hypercalcification23,78,79,80,82. All of these factors would affect buoyancy and locomotion, affecting the ability of young decabrachians to thrive as normal23,24,81. Furthermore, experiments showed that sepiids reared under low feeding conditions develop wider and shorter (more robust) internal shells83, and the PToB event shows evidence for both ocean acidification42 and low productivity62. Because the robustness variation of P. bisulcata correlates significantly with seawater acidification, we suggest that hypercalcification and consequently underfeeding are plausible mechanisms for the morphological changes in P. bisulcata, that should be investigated further.
Given that both of the species that exhibit a significant increase in robustness are affected with arguably differing severity, we propose that P. bisulcata and C. longiforma may have had different thermal and/or bathymetric niches. The thermal tolerance of C. longiforma is unknown, but we argue that, because the higher relative abundance of this species at lower latitude localities84, and because its robustness recovered when temperature at the site decreased after the boundary event, C. longiforma appears better to be able to adapt to warmer habitats than P. bisulcata. Generally, southern range margins of high latitudinal species are believed to be set by interspecific competition, while northern limits are set by physical tolerance85,86. We interpret the responses of P. bisulcata as indicating that this species was adapted to relative cold to temperate environments, which is consistent with their oxygen isotope signals50,55. However, some studies interpreted the family Passaloteuthididae (including both Catateuthis and Passaloteuthis) as ‘all-rounders’ that could easily migrate vertically or spatially, because of the mix of specialised ecomorphological features they have51. Geochemical studies on Passaloteuthis specimens concluded that they preferentially dwelled in deep water without seasonal influence50,55. The epipelagic habitat in Peniche87 and the high abundance of this species during the latest Pliensbachian sea-level regressive phase makes this seem unlikely48. Additionally, the sea-level rise after the Pliensbachian-Toarcian boundary87 likely created accommodation space that should have favoured P. bisulcata, aiding their recovery.
Our study found promising evidence for differential susceptibilities to CRS across the life stages of an extinct epipelagic predator, and possibly varying CRS susceptibility between species modulated by their thermal preferences. Further research on thermal niches and varying sensitivities among life stages in the fossil record can help anticipate CRS vulnerability in modern organisms88 and inform conservation efforts.
Material and methods
930 belemnites were collected during multiple field trips to Peniche (Portugal) in 2016, 2017 and 2018. Specimens were collected from ten consecutive beds in three ammonite zones (Emaciatum, Polymorphum and Levisoni) covering five subzones (Solare, Elisa, Mirabile, Semicelatum and Elegantulum). Two different sampling techniques were employed to reduce the impact of sampling bias48. First, specimens were quantitatively sampled by throwing a 1 m2 frame three times per bed and collecting all belemnites and fragments that were found within that frame. Afterwards 30 complete specimens were sampled from each bed to facilitate taxonomic identification. Taxonomy and ontogenetic stages were identified based on traditionally used morphological traits48,57,84,89,90. Out of the 930 belemnites48,84, all sufficiently preserved specimens were chosen for this study and represent the full samples’ taxonomic composition and ratio of ontogenetic stages well48. The resulting dataset consists of 144 belemnites from seven species in three subfamilies (Table 1, supplementary Fig. S1).
These belemnites were scanned in the micro-CT scanner (v|Tome|X) of the GeoZentrum Nordbayern. After reconstructing the CT scans using VG Studio max, the open source software 3D Slicer91 was used to virtually clean the belemnite rostra from encrusting organisms, adhering sediment and the sediment filling the alveolus. The rostrum cavum was unevenly fractured at different lengths in most specimens. To correct for this taphonomic bias, we cut the rostrum cavum of all 3D models to 1/6th of the length of the rostrum solidum, as this was a length that was preserved in the available specimens. The specimens are stored at the Museu da Ciência da Universidade de Coimbra (Portugal) and the tomographic data can be accessed on MorphoBank (see Data Availability).
To attain proxies for palaeoenvironmental changes across the PToB, we used WebPlotDigitizer-492 to extract δ18O (temperature proxy), δ13C (carbon cycle proxy) and δ11B (seawater pH proxy) values from a geochemical study of the same outcrop42 and assigned them to the corresponding beds in our data48 and consequently all belemnite rostra found within each bed.
The ‘geomorph' R package56 was used to manually place 22 3D-landmarks (Fig. 5) on the belemnite rostra. The landmarks that were placed on the dorsal and ventral sides of the rostra were defined as curve sliders. This means they would orientate themselves equidistantly along the flanks using minimum bending energy and can therefore counteract potential inaccuracies in placing them. A 3D-array, produced with the ‘abind’93 package, of the landmarks of all specimens is used for the General Procrustes Analysis (GPA)56,94. This method rotates and scales the landmarks of all specimens in 3D space to the point of highest similarity. It then calculates the consensus shape (= mean) of all specimens95. The ’Procrustes distances’ between the specimens and the ’consensus shape’ are used to perform a Principal Component Analysis (PCA).
First, we run a linear model on the relationship between the geometric mean and centroid size (supplementary Fig. S3). The former has been used as a body size proxy successfully before48, and centroid size is the size component of a specimen that gets removed during the Procrustes transformation in geometric morphometrics56. This is done to test, whether we could use centroid size as a reliable estimate of body size in this study.
To test for changes in morphology on different levels of assemblage organisation, we use Hedge’s g from the ‘effsize’96 package to quantify changes over time, and assess statistical significance with Mann–Whitney U tests between consecutive samples.
To test, whether a change in species composition of the assemblages has an impact on the morphological pattern, we first use an ANOVA to test for differences in robustness among the species. We subsequently use a bootstrapping method97 to test whether changes in species composition across the subzones are random or not. A vector of the species names was drawn from 29 times (mean sample size on subzone level) with replacement, with the relative abundance of each species in the overall sample defined as the probability for it to be selected. This was repeated 500 times. For each of the 500 randomly calculated samples, the simulated relative abundances for the species were calculated. The range of relative abundances of the individual species is defined as the frame, within which changes in abundance can be considered random.
We then test for a within-species change in robustness, specifically across the boundary. The only two species that cross the boundary are P. bisulcata and C. longiforma. As both these species are known from the full dataset to have a significantly reduced body size after the boundary, we use Mann–Whitney U tests on those species’ centroid sizes between consecutive subzone samples to test whether this works in our subset of specimens. We repeat this to test for changes in robustness (PC1) of these species through time.
To test, whether life stages within the species were affected differently we first employed a linear regression model, using developmental stage as an ordinal predictor variable. The ordinal data structure was used, because it shows trends in smaller data sets more reliably than categorical data structures. We then calculated AICc for both, the null-model and the model containing developmental stage to assess parsimony. Additionally, we run a mood’s median test98 on the robustness of the life stages of both dominant species among subzones. Because of lower sample sizes in the adults of both species, we group adult and neanic specimens together and compare them to juveniles. A post-hoc pairwise median test99 is used to show significance of changes in morphology between consecutive subzones. For all statistical tests we presume that sample size is sufficient, when p-values return at least marginally significant results (< 0.1), as insufficient sampling usually leads to type II errors and does not suggest false positive results. We include marginal significance, as lower p-values are less likely to be produced with smaller data sets.
The hypothesis, that accelerated growth at higher temperatures causes the change in morphology in adult specimens, is tested by examining the relationship between size and robustness in both species. We fit two linear regressions for each species. One for specimens found in beds during times of lower temperature (δ18O > − 0.9) and one for belemnites that lived in a higher temperature environment (δ18O < − 0.9) after the boundary. Low temperature and high temperature is defined based on which stratigraphic beds coincide with hyperthermal pulses of the Pliensbachian-Toarcian crisis. The “low temperature” and “high temperature” models are then compared through an ANOVA.
We use variation partitioning from the ‘vegan’ package100 to calculate the independent effects that environmental changes and lithology have on the morphospace,. For this we use four variables: δ18O, δ13C, δ11B as environmental variables, and lithology (categorical: marl, limestone, marly limestone).
To assess the impact of δ18O, δ13C and δ11B on the robustness on the assemblage scale as well as for P. bisulcata and C. longiforma individually, we use generalised least squares (GLS) modelling from the ‘nlme’ package in R101, correcting for the impact of lithology. This approach was decided on, because it corrects for auto-correlated residuals in the linear model. The chosen environmental variables were proxies for seawater temperature (δ18O), carbon cycle perturbations (δ13C) and seawater acidification (δ11B). The correlation between the variables is < 0.7102 (supplementary Fig. S5). We determine the most parsimonious model using AICc from the ‘nlme’ library in R101, which corrects for uneven sample sizes.
Uncompiled image stacks and mesh files of all belemnites in this study, including specimen identification numbers, have been deposited in a Morphobank repository (http://morphobank.org/permalink/?P3873). The data and R code used for data analysis have been deposited in a GitHub repository and will be made publicly available upon publication (https://github.com/PauSofN/Belemnite_Morphology_modulated_by_life_stages).
Reddin, C. J., Kocsis, Á. T., Aberhan, M. & Kiessling, W. Victims of ancient hyperthermal events herald the fates of marine clades and traits under global warming. Glob. Chang. Biol. 27, 868–878 (2021).
Reddin, C. J., Kocsis, Á. T. & Kiessling, W. Marine invertebrate migrations trace climate change over 450 million years. Glob. Ecol. Biogeogr. 27, 704–713 (2018).
Kordas, R. L., Harley, C. D. G. & O’Connor, M. I. Community ecology in a warming world: The influence of temperature on interspecific interactions in marine systems. J. Exp. Mar. Bio. Ecol. 400, 218–226 (2011).
Poloczanska, E. S. et al. Responses of marine organisms to climate change across oceans. Front. Mar. Sci. 3, 1–21 (2016).
Hanken, J. & Wake, D. B. Miniaturization of body size: Organismal consequences and evolutionary significance. Annu. Rev. Ecol. Syst. 24, 501–519 (1993).
Sheridan, J. A. & Bickford, D. Shrinking body size as an ecological response to climate change. Nat. Clim. Chang. 1, 401–406 (2011).
Forster, J., Hirst, A. G. & Atkinson, D. Warming-induced reductions in body size are greater in aquatic than terrestrial species. Proc. Natl. Acad. Sci. U. S. A. 109, 19310–19314 (2012).
Ohlberger, J. Climate warming and ectotherm body size - from individual physiology to community ecology. Funct. Ecol. 27, 991–1001 (2013).
Garilli, V. et al. Physiological advantages of dwarfing in surviving extinctions in high-CO 2 oceans. Nat. Clim. Chang. 5, 678–682 (2015).
Daufresne, M., Lengfellner, K. & Sommer, U. Global warming benefits the small in aquatic ecosystems. Proc. Natl. Acad. Sci. U. S. A. https://doi.org/10.1073/pnas.0902080106 (2009).
Verberk, W. C. E. P. et al. Shrinking body sizes in response to warming: explanations for the temperature–size rule with special emphasis on the role of oxygen. Biol. Rev. 96, 247–268 (2021).
Angilletta, M. J., Steury, T. D. & Sears, M. W. Temperature, growth rate, and body size in ectotherms: Fitting pieces of a life-history puzzle. Integr. Comp. Biol. 44, 498–509 (2004).
Hoving, H. J. T. et al. Extreme plasticity in life-history strategy allows a migratory predator (jumbo squid) to cope with a changing climate. Glob. Chang. Biol. 19, 2089–2103 (2013).
Angilletta, M. J. & Dunham, A. E. The temperature-size rule in ectotherms: simple evolutionary explanations may not be general. Am. Nat. 162, 332–342 (2003).
Vinarski, M. V. On the applicability of Bergmann’s rule to ectotherms: the state of the art. Biol. Bull. Rev. 4, 232–242 (2014).
Atkinson, D. Temperature and organism size: a biological law for organisms?. Adv. Ecol. Res. 25, 1–58 (1994).
Atkinson, D. Effects of temperature on the size of aquatic ectotherms: Exceptions to the general rule. J. Therm. Biol. 20, 61–74 (1995).
Forster, J. & Hirst, A. G. The temperature-size rule emerges from ontogenetic differences between growth and development rates. Funct. Ecol. 26, 483–492 (2012).
Gillooly, J. F., Brown, J. H., West, G. B., Savage, V. M. & Charnov, E. L. Effects of size and temperature on metabolic rate. Science 293, 2248–2251 (2001).
Schulte, P. M. The effects of temperature on aerobic metabolism: Towards a mechanistic understanding of the responses of ectotherms to a changing environment. J. Exp. Biol. 218, 1856–1866 (2015).
Gillooly, J. F., Charnov, E. L., West, G. B., Savage, V. M. & Brown, J. H. Effects of size and temperature on developmental time. Nature 417, 70–73 (2002).
Riemer, K., Anderson-Teixeira, K. J., Smith, F. A., Harris, D. J. & Ernest, S. K. M. Body size shifts influence effects of increasing temperatures on ectotherm metabolism. Glob. Ecol. Biogeogr. 27, 958–967 (2018).
Rosa, R. et al. Ocean warming enhances malformations, premature hatching, metabolic suppression and oxidative stress in the early life stages of a keystone squid. PLoS ONE 7, e38282 (2012).
Pecl, G. T. & Jackson, G. D. The potential impacts of climate change on inshore squid: Biology, ecology and fisheries. Rev. Fish Biol. Fish. 18, 373–385 (2008).
Twitchett, R. J. The Lilliput effect in the aftermath of the end-Permian extinction event. Palaeogeogr. Palaeoclimatol. Palaeoecol. 252, 132–144 (2007).
Harries, P. J. & Knorr, P. O. What does the ‘Lilliput Effect’ mean?. Palaeogeogr. Palaeoclimatol. Palaeoecol. 284, 4–10 (2009).
Metcalfe, B., Twitchett, R. J. & Price-Lloyd, N. Changes in size and growth rate of ‘Lilliput’ animals in the earliest Triassic. Palaeogeogr. Palaeoclimatol. Palaeoecol. 308, 171–180 (2011).
Chu, D. et al. Lilliput effect in freshwater ostracods during the Permian-Triassic extinction. Palaeogeogr. Palaeoclimatol. Palaeoecol. 435, 38–52 (2015).
Urbanek, A. Biotic crises in the history of upper silurian graptoloids: a palaeobiological model. Hist. Biol. https://doi.org/10.1080/10292389309380442 (1993).
Urlichs, M. Stunting in invertebrates from the type area of the Cassian Formation (Early Carnian) of the dolomites (Italy). GeoAlp 8, 164–169 (2011).
Morten, S. D. & Twitchett, R. J. Fluctuations in the body size of marine invertebrates through the Pliensbachian-Toarcian extinction event. Palaeogeogr. Palaeoclimatol. Palaeoecol. https://doi.org/10.1016/j.palaeo.2009.08.023 (2009).
Piazza, V., Ullmann, C. V. & Aberhan, M. Temperature-related body size change of marine benthic macroinvertebrates across the Early Toarcian Anoxic Event. Sci. Rep. 10, 1–13 (2020).
Calosi, P., Putnam, H. M., Twitchett, R. J. & Vermandele, F. Marine metazoan modern mass extinction: improving predictions by integrating fossil, modern, and physiological data. Ann. Rev. Mar. Sci. 11, 369–390 (2019).
Gerber, S. Comparing the differential filling of morphospace and allometric space through time: the morphological and developmental dynamics of Early Jurassic ammonoids. Paleobiology 37, 369–382 (2011).
Pálfy, J. & Smith, P. L. Synchrony between Early Jurassic extinction, oceanic anoxic event, and the Karoo-Ferrar flood basalt volcanism. Geology 28, 747–750 (2000).
Caruthers, A. H., Smith, P. L. & Gröcke, D. R. The Pliensbachian-Toarcian (Early Jurassic) extinction, a global multi-phased event. Palaeogeogr. Palaeoclimatol. Palaeoecol. 386, 104–118 (2013).
Wignall, P. B. Large igneous provinces and mass extinctions. Earth Sci. Rev. 53, 1–33 (2001).
Percival, L. M. E. et al. Globally enhanced mercury deposition during the end-Pliensbachian extinction and Toarcian OAE: A link to the Karoo-Ferrar Large Igneous Province. Earth Planet. Sci. Lett. 428, 267–280 (2015).
Foster, G. L., Hull, P., Lunt, D. J. & Zachos, J. C. Placing our current ‘hyperthermal’ in the context of rapid climate change in our geological past. Phil. Trans. R. Soc. A 376, 20170086 (2018).
Suan, G. et al. Secular environmental precursors to Early Toarcian (Jurassic) extreme climate changes. Earth Planet. Sci. Lett. 290, 448–458 (2010).
Fantasia, A. et al. Global versus local processes during the Pliensbachian-Toarcian transition at the Peniche GSSP, Portugal: A multi-proxy record. Earth Sci. Rev. 198, 102932 (2019).
Müller, T. et al. Ocean acidification during the early Toarcian extinction event: Evidence from Boron isotopes in brachiopods. Geology 48, 1184–1188 (2020).
Suan, G., Mattioli, E., Pittet, B., Mailliot, S. & Lécuyer, C. Evidence for major environmental perturbation prior to and during the Toarcian (Early Jurassic) oceanic anoxic event from the Lusitanian Basin Portugal. Paleoceanography 23, A1202 (2008).
Dera, G. et al. High-resolution dynamics of early Jurassic marine extinctions: The case of Pliensbachian-Toarcian ammonites (Cephalopoda). J. Geol. Soc. London 167, 21–33 (2010).
Dera, G. et al. Climatic ups and downs in a disturbed Jurassic world. Geology 39, 215–218 (2011).
Miguez-Salas, O., Rodríguez-Tovar, F. J. & Duarte, L. V. Selective incidence of the Toarcian oceanic anoxic event on macroinvertebrate marine communities: a case from the Lusitanian basin Portugal. Lethaia 50, 548–560 (2017).
Correia, V. F., Riding, J. B., Duarte, L. V., Fernandes, P. & Pereira, Z. The palynological response to the Toarcian Oceanic Anoxic Event (Early Jurassic) at Peniche, Lusitanian Basin, western Portugal. Mar. Micropaleontol. 137, 46–63. https://doi.org/10.1016/j.marmicro.2017.10.004 (2017).
Rita, P., Nätscher, P., Duarte, L. V., Weis, R. & De Baets, K. Mechanisms and drivers of belemnite body-size dynamics across the Pliensbachian-Toarcian crisis. R. Soc. Open Sci. 6, 190494 (2019).
Caswell, B. A. & Coe, A. L. The impact of anoxia on pelagic macrofauna during the Toarcian Oceanic Anoxic Event (Early Jurassic). Proc. Geol. Assoc. 125(4), 383–391. https://doi.org/10.1016/j.pgeola.2014.06.001 (2014).
Ullmann, C. V., Thibault, N., Ruhl, M., Hesselbo, S. P. & Korte, C. Effect of a Jurassic oceanic anoxic event on belemnite ecology and evolution. Proc. Natl. Acad. Sci. U. S. A. 111, 10073–10076 (2014).
Dera, G., Toumoulin, A. & de Baets, K. Diversity and morphological evolution of Jurassic belemnites from South Germany. Palaeogeogr. Palaeoclimatol. Palaeoecol. 457, 80–97 (2016).
Neige, P., Weis, R. & Fara, E. Ups and downs of belemnite diversity in the Early Jurassic of Western Tethys. Palaeontology 64, 263–283 (2021).
Rita, P., De Baets, K. & Schlott, M. Rostrum size differences between Toarcian belemnite battlefields. Foss. Rec. 21, 171–182 (2018).
Rita, P. et al. Biogeographic patterns of belemnite body size responses to episodes of environmental crisis. PeerJ Prepr. (2019).
Hoffmann, R. & Stevens, K. The palaeobiology of belemnites – foundation for the interpretation of rostrum geochemistry. Biol. Rev. 95, 94–123 (2020).
Adams, D. C. & Otárola-Castillo, E. Geomorph: An r package for the collection and analysis of geometric morphometric shape data. Methods Ecol. Evol. 4, 393–399 (2013).
Schlegelmilch, R. Die Belemniten des süddeutschen Jura. Die Belemniten des süddeutschen Jura https://doi.org/10.1007/978-3-8274-3083-0 (1998).
McArthur, J. M. et al. Sr-isotope stratigraphy (87Sr/86Sr) of the lowermost Toarcian of Peniche, Portugal, and its relation to ammonite zonations. Newsletters Stratigr. 53, 297–312 (2020).
Hesselbo, S. P., Jenkyns, H. C., Duarte, L. V. & Oliveira, L. C. V. Carbon-isotope record of the Early Jurassic (Toarcian) Oceanic Anoxic Event from fossil wood and marine carbonate (Lusitanian Basin, Portugal). Earth Planet. Sci. Lett. 253, 455–470 (2007).
Klug, C., Schweigert, G., Fuchs, D., Kruta, I. & Tischlinger, H. Adaptations to squid-style high-speed swimming in Jurassic belemnitids. Biol. Lett. 12, 20150877 (2016).
Seibel, B. A., Thuesen, E. V., Childress, J. J. & Gorodezky, L. A. Decline in pelagic cephalopod metabolism with habitat depth reflects differences in locomotory efficiency. Biol. Bull. 192, 262–278 (1997).
Mattioli, E., Pittet, B., Petitpierre, L. & Mailliot, S. Dramatic decrease of pelagic carbonate production by nannoplankton across the Early Toarcian anoxic event (T-OAE). Glob. Planet. Change 65, 134–145 (2009).
Chamberlain, J. A. Locomotion in ancient seas: Constraint and opportunity in Cephalopod adaptive design. Geobios 15, 49–61 (1993).
Rexfort, A. & Mutterlose, J. The role of biogeography and ecology on the isotope signature of cuttlefishes (Cephalopoda, Sepiidae) and the impact on belemnite studies. Palaeogeogr. Palaeoclimatol. Palaeoecol. 284, 153–163 (2009).
Holland, S. M. The quality of the fossil record: a sequence stratigraphic perspective. Paleobiology 26, 148–168 (2000).
Holland, S. M. The non-uniformity of fossil preservation. Philos. Trans. R. Soc. B Biol. Sci. 371, 2 (2016).
Korn, D. Impact of enviornmental perturbations o heterochronic develpments in Palaeozoic ammonoids. Evol. Chang. Heterochrony 245–260 (1995).
Yacobucci, M. M. Plasticity of developmental timing as the underlying cause of high speciation rates in ammonoids. in Advancing research on living and fossil cephalopods 59–76 (Springer, Boston, MA, 1999).
Landman, N. H. & Gyssant, J. R. Heterochrony and ecology in Jurassic and Cretaceous ammonites. Geobios 26, 247–255 (1993).
McNamara, K. J. Heterochrony: the evolution of development. Evol. Educ. Outreach 5, 203–218 (2012).
Dahlke, F. T., Wohlrab, S., Butzin, M. & Pörtner, H. O. Thermal bottlenecks in the life cycle define climate vulnerability of fish. Science 369, 65–70 (2020).
Pörtner, H. O. & Farrell, A. P. Ecology: Physiology and climate change. Science https://doi.org/10.1126/science.1163156 (2008).
Pimentel, M. S. et al. Impact of ocean warming on the early ontogeny of cephalopods: A metabolic approach. Mar. Biol. 159, 2051–2059 (2012).
Komoroske, L. M. et al. Ontogeny influences sensitivity to climate change stressors in an endangered fish. Conserv. Physiol. 2, 1–13 (2014).
Pörtner, H. O., Bock, C. & Mark, F. C. Oxygen- & capacity-limited thermal tolerance: Bridging ecology & physiology. J. Exp. Biol. 220, 2685–2696 (2017).
Harnik, P. G., Simpson, C. & Payne, J. L. Long-term differences in extinction risk among the seven forms of rarity. Proc. R. Soc. B Biol. Sci. 279, 4969–4976 (2012).
Reddin, C. J., Kocsis, Á. T. & Kiessling, W. Climate change and the latitudinal selectivity of ancient marine extinctions. Paleobiology 45, 70–84 (2019).
Dorey, N. et al. Ocean acidification and temperature rise: Effects on calcification during early development of the cuttlefish Sepia officinalis. Mar. Biol. 160, 2007–2022 (2013).
Sigwart, J. D. et al. Elevated pCO2 drives lower growth and yet increased calcification in the early life history of the cuttlefish Sepia officinalis (Mollusca: Cephalopoda) Julia. ICES J. Mar. Sci. 73, 970–980 (2016).
Gutowska, M. A., Melzner, F., Pörtner, H. O. & Meier, S. Cuttlebone calcification increases during exposure to elevated seawater pCO2 in the cephalopod Sepia officinalis. Mar. Biol. 157, 1653–1663 (2010).
Kaplan, M. B., Mooney, T. A., McCorkle, D. C. & Cohen, A. L. Adverse effects of ocean acidification on early development of squid (Doryteuthis pealeii). PLoS ONE 8, e63714 (2013).
Otjacques, E. et al. Cuttlefish buoyancy in response to food availability and ocean acidification. Biology (Basel). https://doi.org/10.3390/biology9070147 (2020).
Neige, P. & Boletzky, S. Morphometrics of the shell of three Sepia species (Mollusca: Cephalopoda): Intra- and interspecific variation. Zool. Beitraege. 38, 137–156 (1997).
Rita, P., Weis, R., Duarte, L. V. & De Baets, K. Taxonomical diversity and palaeobiogeographical affinity of belemnites from the Pliensbachian-Toarcian GSSP (Lusitanian Basin, Portugal). Pap. Palaeontol. https://doi.org/10.1002/spp2.1343 (2020).
MacArthur, R. H. Geographical ecology: patterns in the distribution of species. (Princeton University Press, 1972).
Gaston, K. J. The structure and dynamics of geographic ranges. (Oxford University Press on Demand, 2003).
Duarte, L. Sequence stratigraphy and depositional setting of the Pliensbachian and Toarcian marly limestones in the Lusitanian Basin Portugal. Ciências da Terra 16, 17–23 (2007).
Reddin, C. J., Nätscher, P. S., Kocsis, Á. T., Pörtner, H.-O. & Kiessling, W. Marine clade sensitivities to climate change conform across timescales. Nat. Clim. Chang. 10, 249–253 (2020).
Doyle, P. & Kelly, R. A. The Jurassic and Cretaceous belemnites of Kong Karls Land, Svalbard. (Norsk Polarinstitutt Oslo, 1988).
Doyle, P. New records of dimitobelid belemnites from the cretaceous of james ross island Antarctica. Alcheringa 14, 159–175 (1990).
Fedorov, A. et al. 3D Slicer as an image computing platform for the Quantitative Imaging Network. Magn. Reson. Imaging https://doi.org/10.1016/j.mri.2012.05.001 (2012).
Rohatgi, A., Rehberg, S. & Stanojevic, Z. Webplotdigitizer: Version 4.1 of Webplotdigitizer. (2018).https://doi.org/10.5281/zenodo.1137880.
Plate, T. & Heiberger, R. Package ‘ abind ’. (2016).
Collyer, M. L. & Adams, D. C. RRPP: An r package for fitting linear models to high-dimensional data using residual randomization. Methods Ecol. Evol. 9, 1772–1779 (2018).
Sherratt, E. Quick Guide to Geomorph v. 2.0. public.iastate.edu (2014).
Torchiano, M. Package ‘ effsize ’. (2020).
Gotelli, N. J., Dorazio, R. M., Ellison, A. M. & Grossman, G. D. Detecting temporal trends in species assemblages with bootstrapping procedures and hierarchical models. Philos. Trans. 365, 3621–3631 (2010).
Hervé, M. Package ‘ RVAideMemoire ’. (2021).
Mangiafico, S. Package ‘ rcompanion ’. (2021).
Oksanen, J. et al. Package ‘vegan’ title community ecology package. Commun. Ecol. Packag. 2, 1–297 (2019).
Pinheiro, J. et al. Package ‘nlme’. (2021).
Dormann, C. F. et al. Collinearity: A review of methods to deal with it and a simulation study evaluating their performance. Ecography (Cop.) 36, 27–46 (2013).
R Core Team. R: A Language and Environment for Statistical Computing. Vienna, Austria (2019).
Garnier, S., Ross, N., Rudis, B. & Sciaini, M. Package ‘viridis’. (2021).
This research was performed in the framework of the DFG Research Unit TERSANE (FOR 2332; grant numbers BA 5148/1‐1, BA 5148/1‐2 to PD Dr. Kenneth De Baets, and AB 109/11-1 to PD Dr. Martin Aberhan) and IGCP 655 (IUGS–UNESCO). We want to thank Alejandra Gomez and Christian Schulbert (FAU) for CT-scanning belemnites and Dr. Emilia Jarochowska for insightful discussions. We are greatly indebted to Luís Vítor Duarte who guided us through the nice outcrops during the expeditions to collect the belemnite specimens, Birgit Leipner-Mata for helping to prepare our specimens and the Museu da Ciência da Universidade de Coimbra (Portugal) for safeguarding the specimens.
Open Access funding enabled and organized by Projekt DEAL.
The authors declare no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Nätscher, P.S., Dera, G., Reddin, C.J. et al. Morphological response accompanying size reduction of belemnites during an Early Jurassic hyperthermal event modulated by life history. Sci Rep 11, 14480 (2021). https://doi.org/10.1038/s41598-021-93850-0
This article is cited by
Swiss Journal of Palaeontology (2021)