Abstract
Activin, a member of the transforming growth factor-β (TGF-β) superfamily of proteins, induces various tissues from the amphibian presumptive ectoderm, called animal cap explants (ACs) in vitro. However, it remains unclear how and to what extent the resulting cells recapitulate in vivo development. To comprehensively understand whether the molecular dynamics during activin-induced ACs differentiation reflect the normal development, we performed time-course transcriptome profiling of Xenopus ACs treated with 50 ng/mL of activin A, which predominantly induced dorsal mesoderm. The number of differentially expressed genes (DEGs) in response to activin A increased over time, and totally 9857 upregulated and 6663 downregulated DEGs were detected. 1861 common upregulated DEGs among all Post_activin samples included several Spemann’s organizer genes. In addition, the temporal transcriptomes were clearly classified into four distinct groups in correspondence with specific features, reflecting stepwise differentiation into mesoderm derivatives, and a decline in the regulation of nuclear envelop and golgi. From the set of early responsive genes, we also identified the suppressor of cytokine signaling 3 (socs3) as a novel activin A-inducible gene. Our transcriptome data provide a framework to elucidate the transcriptional dynamics of activin-driven AC differentiation, reflecting the molecular characteristics of early normal embryogenesis.
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Introduction
Embryogenesis is precisely regulated by various inductive interactions leading to tissue- or organ-specific gene expression programs and generation of cell fate diversity. In amphibian development, mesoderm induction is one of the primary embryonic interactions. During this process, a signal released from vegetal cells (the presumptive endoderm) converts the ectoderm into the mesodermal fate, establishing the three germ layers. Since the discovery of mesoderm formation depending on inductive events, the identification of mesoderm-inducing substances and their functions have been investigated1,2.
Activin has been identified as a potential mesoderm inducing factor based on its capacity to induce into the mesodermal and endodermal lineage in vitro. Following treatment with activin in combination with other morphogenic proteins such as bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs), embryonic stem cells are differentiated into mesoderm or definitive endoderm3,4,5,6. In amphibian experiments, animal cap explants (ACs) isolated from the animal pole region of blastulae differentiate autonomously into atypical epidermis, but they can differentiate into various endodermal and mesodermal tissues upon activin treatment7,8,9,10. As for mesoderm induction, activin at higher concentrations induces dorsal types such as muscle and notochord, and at lower concentrations induces ventral type in ACs10. This concentration-dependent tissue induction is due to the behavior of activin as a morphogen in dorsoventral patterning of mesoderm11,12,13. One of the fascinating features of activin-treated ACs is their ability to mimic the activity of the signaling center, Spemann’s organizer. After the transplantation of activin-treated ACs into the blastocoel of another embryo, a secondary axis is induced, similar to transplantation experiments of the organizer dissected from the embryo14. Thus, activin-treated ACs can mimic the organizer’s activity, but it remains unclear how and to what extent ACs differentiation in vitro recapitulates the organizer formation in vivo.
The recently developed deep RNA sequencing (RNA-seq) provides transcriptional information with a high resolution and sensitivity. This technique has been widely applied to cultured cells, model organisms, as well as to gastruloids and organoids of developmental models15,16,17,18. In Xenopus research, RNA-seq protocols and pipelines for data analysis have been developed in recent years, and several reports using this approach have been published19,20,21,22. In particular, transcriptome analysis of the dorsal marginal zones in Xenopus gastrula that contain dorsal mesoderm populations has been useful for understanding mesoderm developmental in vivo23,24.
In the present study, we performed a time-course transcriptome profile of Xenopus ACs treated with 50 ng/mL of activin A, which was known to predominantly induce dorsal mesoderm10. We identified stepwise variation in gene expression, as well as the enriched biological processes, during activin A-driven AC differentiation. From the results, we detected several known organizer genes, and captured the transition from early unrestricted gene expression to mesoderm lineage commitment later. Furthermore, we found that activin A treatment resulted in the potent activation of the early-responsive genes, but the gene expression patterns of late-responsive genes in ACs were similar to those in the gastrula embryos25. We further reported socs3 as a novel activin A-inducible gene and inferred the socs3-associated genes. Thus, our data provide information of the temporal dynamics in gene expression during activin A-induced ACs differentiation, and further support the usefulness of the in vitro ACs assays for developmental studies.
Results
Validation of mesoderm-inducing activity by activin A treatment
We first validated the effective concentrations of activin A for the induction of dorsal mesoderm genes by semiquantitative RT-PCR. As shown in Supplementary Fig. S1, 50 ng/mL activin A predominantly induced dorsal mesoderm genes, compared with 10 ng/mL activin A, which is consistent with previous findings that higher concentrations of activin induce dorsal mesoderm11,12,13. We then validated the ability of activin A at 50 ng/mL concentration to induce dorsal mesoderm at later time points. As shown in Supplementary Fig. S2A, activin A-treated ACs exhibited extensive elongation, which is reportedly caused by the differentiation of notochord and somite10. Furthermore, we found that the expression of the notochord marker, chrd, and the somite markers, myf5, actc1 and myogenin were induced after 48 h of activin A treatment (Supplementary Fig. S2B). These data indicate that 50 ng/mL activin A is the optimal concentration for ACs differentiation into dorsal mesoderm.
Transcriptomic changes in activin A-treated ACs
To comprehensively understand the temporal gene expression during activin A-induced ACs differentiation, we collected ACs immediately after dissection from blastula (Pre_activin; sibling embryos reached stages 8.5–9), and ACs after the cultivation in activin A solution for 1, 3, 6 and 9 h (Post 1h_activin; stage 9.5, Post 3h_activin; stages 10–10.25, Post 6h_activin; stages 10.5–11, and Post 9h_activin; stage 11.5, respectively) (Fig. 1A), and performed a time-course RNA-seq to analyze the transcriptome. To assess the dynamics of gene expression variability following activin A treatment, we performed principal component analysis (PCA) and hierarchical clustering analysis. PCA using all samples showed that there was a clear distinction between the same groups at five time points, and the transcriptomic changes in ACs from Pre_activin to Post 9h_activin (Fig. 1B). Moreover, in hierarchical clustering analysis, we found that Pre_activin samples were distinguished from Post_activin groups (Post 1 h, 3 h, 6 h, and 9h_activin), which were further divided into two subgroups, short-term (Post 1 h and 3h_activin) and long-term activin A treatment (Post 6 h and 9h_activin) (Fig. 1C). These data suggest that the gene expression of ACs is significantly changed after activin A treatment in a temporal manner.
We then validated the accuracy of gene expression patterns observed by RNA-seq with a semiquantitative RT-PCR. We focused on ccnb1 as a representative downregulated gene, and eomesodermin (eomes), lhx1, myf5, t, goosecoid (gsc), otx2, chordin (chrd), wnt8a and cerberus 1 (cer1) as induced genes upon activin A treatment26,27,28,29,30,31. The temporal expression patterns of these representative genes observed by RT-PCR were similar to the data from RNA-seq, with minor differences (compare Supplementary Fig. S3B to S3C, and blue lines in Supplementary Fig. S1 to Supplementary Fig. S4).
Differentially expressed genes profiles between Pre_activin and Post_activin samples
We next analyzed the statistically significant differentially expressed genes (DEGs) between activin A-treated and -untreated samples. Based on a false discovery rate (FDR) of less than 0.05 and a log2 fold change (FC) ≥ 2 or ≤ − 2, we extracted DEGs in Post_activin samples compared to the Pre_activin sample as a control. The full list of DEGs is shown in Supplementary Table S1, and the top five upregulated and downregulated DEGs in each comparison are shown in Table 1. As shown in Fig. 2A, volcano plots showed that 2573, 4149, 6323, and 8858 genes were upregulated, and 111, 1901, 4704 and 6008 genes were downregulated in the Post 1 h, 3 h, 6 h, and 9h_activin samples, respectively. Thus, the number of DEGs gradually increased over time after treatment with activin A, but more upregulated DEGs were detected than downregulated DEGs across all time points (Fig. 2B). As shown in Fig. 2C, the ratio of unique DEGs (non-overlapping DEGs among different Post_activin samples) in Post 9h_activin sample was higher than other Post_activin samples, implying that time point- or context-specific gene expression actively occurs at least 9 h after treatment with activin A.
According to the data set of DEGs shown in the Venn diagrams, 1861 genes including activin-responsive targets, mix1, gsc, eomes, foxa4 and nodal132,33,34,35,36, were commonly upregulated (Fig. 3A and Supplementary Table S2), and only 70 genes were commonly downregulated among all Post_activin samples (Supplementary Fig. S5 and Supplementary Table S2). To characterize the functional features of DEGs in ACs following activin A treatment, we performed gene ontology (GO) functional annotation of common upregulated DEGs. Common upregulated DEGs among all Post_activin samples were associated with animal organ morphogenesis (GO:0009887) and embryonic organ development (GO:0048568) (Fig. 3B).
To gain the understanding of genes induced by activin A, we compared the DEGs in response to activin A with the set of genes enriched in the marginal zone of the Xenopus gastrula. The transcriptome datasets of dorsal marginal zone (DMZ)- and ventral marginal zone (VMZ)-enriched genes were obtained from different groups23,24, and compared with common upregulated DEGs among all Post_activin samples. As shown in Fig. 3C, we detected the overlaps of DMZ-enriched genes including gsc.L, lhx1.L, foxa4, fzd8, and follistatin (fst.L) that are involved in Spemann’s organizer formation, as well as ccnd1.L encoding cyclin D1, and sds.L encoding serine dehydratase (left). In addition, a few VMZ-enriched genes including wnt8a.L, dlx3.S, and trim29.L overlapped with upregulated DEGs in response to activin A (Fig. 3C right).
Classification of genes that exhibited distinct expression pattern
To identify the set of genes with similar temporal expression patterns, we performed k-means clustering for 2000 genes, which were variably expressed after activin A treatment. We identified four clusters with distinct expression patterns (Fig. 4A and Supplementary Table S3). Cluster A (967 genes) contained a set of genes that was downregulated over time after activin A treatment. Clusters B, C, and D contained genes that were upregulated after activin A treatment, but there was little difference in gene expression patterns. Cluster B (213 genes) contained genes whose expression was significantly upregulated at 1 or 3 h and subsequently downregulated at later time points. Cluster C (204 genes) contained genes whose expression was markedly from 3 or 6 h, and slightly downregulated but maintained at higher expression levels at later time points. Cluster D (616 genes) contained genes that were drastically upregulated at 6 or 9 h.
To compare activin A-induced ACs differentiation in vitro with in vivo development, we presented the temporal gene expression of five representatives of each cluster in activin A-treated ACs and that in whole gastrula embryos. The transcriptome dataset of the whole gastrula embryos was obtained from published RNA-seq data25. As shown in Fig. 4B, the downregulation of genes in cluster A was observed in both cultured ACs and whole embryos. Conversely, the expression of genes in cluster B was actively induced in ACs at the early time point (in Post 3 h and 6h_activin ACs), but these genes were modestly induced in whole embryos (Fig. 4B). The expression of genes in cluster C and cluster D increased in ACs later (in Post 9h_activin ACs), and similar temporal changes in gene expression were observed in whole embryos (Fig. 4B).
To further investigate the functional diversity of DEGs over time after activin A treatment, we next performed functional enrichment analysis on each cluster. The top ten GO terms in each cluster are listed in Supplementary Table S4. GO enrichment analysis of biological processes showed that genes in cluster A were associated with nuclear envelop organization (GO:0006998), regulation of endoplasmic reticulum (GO:1900102) and golgi (GO:0090166, GO:0048313) (Fig. 4C and Supplementary Table S4). Notably, genes in cluster B were associated with growth factor stimulus (GO:009719, GO:0071363, GO:0071495, GO:0070848), in cluster C were associated with primary germ layer formation (GO:0009798, GO:0007492, GO:0007498, GO:0001704, GO:0001705), and those in cluster D were associated with regionalization (GO:0003002), pattern specification (GO:0007389, GO:0009952), and somite formation (GO:0001756, GO:0061053) (Fig. 4C and Supplementary Table S4). In addition, the predicted protein–protein interaction (PPI) network for genes in cluster C showed that some genes, encoding transcription factors such as OTX2, SOX17, T, and LHX1, were assigned in the mesoderm development GO term (GO: 0007498) (Fig. 4C pink circles, Supplemental Table S5).
Upregulation of socs3 in response to activin A
The most significantly upregulated DEGs in activin A-treated ACs at the early time point was socs3 (Table 1, Post 1h_activin). Therefore, we focused on socs3 as a possible activin-inducible gene. socs3 is reportedly involved in cytokine signaling, and induced in response to wound healing after epithelium trauma37. To determine whether socs3 is upregulated in response to activin A treatment, we examined the temporal expression of socs3 in ACs with or without activin A treatment using semiquantitative RT-PCR. As shown in Fig. 5, socs3 was slightly expressed in ACs before the cultivation (− activin, 0 h), but significantly upregulated after 1 and 3 h of activin A treatment (+ activin, 1 h and 3 h), compared with in the absence of activin A treatment at the same time points (− activin, 1 h and 3 h). These data suggest that socs3 was maternally expressed or induced in response to the traumatic dissection from blastulae, but further induced after activin A treatment. Thus, we validated that RNA-seq can be used to identify novel activin-inducible genes in ACs. To predict the socs3-associated genes in activin A-treated ACs, we inferred a gene network using temporal gene expression profiles of upregulated DEGs in Post 3h_activin samples. In the inferred network, we found many potential socs3.S-associated genes including pou5f3.2.S, gata4.S, and map3k1.S (Supplementary Fig. S6, Supplementary Table S6).
Discussion
In this study, we captured the transcriptional features of ACs that had the potential to develop into dorsal mesoderm. Although the transcriptional organization in activin A-treated ACs did not completely correspond to DMZ-expressing genes, activin A-treated ACs expressed a variety of dorsal mesoderm genes that are involved in Spemann’s organizer formation. We also found that temporal gene expression patterns were similar between activin A-treated ACs and whole embryos, with some notable differences. We also provided the data on expression of socs3 as a potential activin-inducible gene, which was identified from our RNA-seq dataset.
In the present study, we confirmed that mix1, gsc, eomes, foxa4 and nodal1 were rapidly upregulated, and lhx1, otx2, chrd, cer1, wnt8, and ventx were also upregulated following activin A treatment (Supplementary Tables S1 and S2), as previously reported27,29,30,31,32,33,34,35,36,38. In Xenopus gastrula embryos, these genes are expressed in the Spemann’s organizer and the ventral mesoderm (Supplementary Fig. S7), and play important roles in the endoderm and mesoderm formation39. Although activin signaling acts as a morphogen and induces both ventral and dorsal mesoderm11,12,13, the upregulated DEGs following 50 ng/mL activin A treatment in ACs overlapped with DMZ-enriched genes more than VMZ-enriched genes (Fig. 3C). These results suggest that this concentration of activin A was sufficient to induce and maintain the dorsal mesoderm.
Activin signaling regulates other signaling pathways for mesoderm development in Xenopus. For example, activin reportedly induces the expression of fgf3/8, and also induces the negative regulator of FGF signaling, dusp1, for the dorsoventral patterning of mesoderm40. Furthermore, activin signaling reportedly activates the expression of downstream genes of p53 signaling, p21waf1 and pal-1, and the mesendoderm gene, mix.2, through the cooperative action with p5341,42,43. Consistent with the findings of previous reports, we confirmed the induction of these genes (Supplementary Tables S1 and S2), and detected the relevance of upregulated DEGs to FGF and p53 signaling from the GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis (Supplementary Table S4). Thus, activin signaling can modulate other signaling pathways for establishment of mesoderm in ACs. Notably, the expression of siamois (sia1 and sia2) that acts downstream of Wnt signaling and is essential for the formation of the Spemann’s organizer44 was not significantly induced in activin A-treated ACs (Supplementary Table S7). Furthermore, all previously reported DMZ-enriched genes did not overlap with upregulated DEGs in response to activin A (Fig. 3C). These differences in gene expression between differentiated ACs and embryonic development are possibly due to the limited effects of activin A at this concentration in ACs, or due to other signaling derived from the neighboring cells in the embryo, but are not in ACs.
In this study, we classified upregulated genes whose expression reached the peak at early (Post 1 h and 3 h), intermediate (Post 3 h and 6 h), and later time points (Post 6 h and 9 h) following activin A treatment (Fig. 4A; corresponding to cluster B, C and D). The expression of activin-early responsive genes (cluster B) was actively induced in ACs than in whole gastrula embryos (Fig. 4B). In addition, the temporal trends in expression of intermediate- or late-responsive genes (clusters C and D) in ACs were highly consistent with those observed in gastrulae, although there were some differences in transcription levels and temporal association (Fig. 4B). These data imply that the primary or direct effects of activin A on early-responsive genes result in the downstream gene expression in concordance with normal development. As for the activin-responsive transcription, several activin-responsive targets were identified from the experiments using the combinatorial treatment of activin and the protein synthesis inhibitor, cycloheximide26,27,33. In addition, genes associated with transcription factors of activin/nodal signaling, Smad2/3 and FoxhI, were determined by the chromatin immunoprecipitation sequencing (ChIP-seq) using X. tropicalis gastrulae45,46. We confirmed that several activin-responsive target genes and Smad2/3-FoxhI associated genes were upregulated in ACs at early time points after activin A treatment (Supplementary Table S3 and Supplementary Fig. S8). It suggests the possibility that the effects of activin A on direct target genes lead to the induction of indirect or secondary responsive genes, resulting in diverse gene expressions in ACs later.
Interestingly, numerous genes previously undescribed in mesoderm formation were observed. Especially, downregulated genes in ACs following activin A treatment have not been investigated well in former experiments, as compared to upregulated genes (Table 1). We first demonstrated that genes associated with the organization of nuclear envelop were downregulated in ACs following activin A treatment, for example, ctdnep1 and lemd3 (Fig. 4C and Supplementary Table S4). CTDNEP1, encoding a serine/threonine protein phosphatase, functions as a mediator of lipid composition in nuclear envelop, but it represses both TGF-β and BMP signal transduction47,48,49,50. Lemd3, encoding a nuclear membrane protein, also suppresses the TGF-β signal transduction through physical interaction with downstream transcription factors of TGF-β signaling, Smads51,52. These data suggest the possibility that the nuclear envelop is organized during ACs differentiation into the endodermal and mesodermal lineage, leading to reinforcement of the TGF- β signal activation. The role of downregulated genes associated with the regulation of golgi and endoplasmic reticulum and rRNA/tRNA transcription in early developmental processes remains to be elucidated (Fig. 4C), but our data may lead to the identification of novel molecular mechanisms in vivo development.
During the blastula stage of embryonic development, zygotic gene activation occurs, which is called the mid-blastula transition (MBT)53,54. It has been reported that the open chromatin accessibility for gene expression becomes higher after MBT (at least after stage 9) in Xenopus ACs55. Our transcriptome data using ACs dissected from blastula demonstrated the dynamic expression of various epigenetic regulators, which increase chromatin accessibility and lead to acceleration of gene transcription. For example, various hdac genes, encoding histone deacetylases, were downregulated (except for hdac9.S, hdac10.L, and hdac7), whereas ep300 and hat1, encoding histone acetyltransferase, were upregulated in response to activin A treatment (Supplementary Tables S1 and S2). In general, histone acetylation targeted to lysines progressively increases transcriptional activation, whereas HDAC deacetylation activity represses transcription56. Our data demonstrating downregulation of hdac and upregulation of ep300 and hat suggest the possibility that gene expression is activated by epigenetic mechanisms in ACs following activin A treatment. Another example of epigenetic regulators that alter the expression levels in response to activin A treatment is dnmt1, encoding DNA methyltransferase (Supplementary Tables S1 and S2). During normal development, Dnmt1 suppresses premature gene activation before MBT57, and the expression levels are downregulated from late blastula to early gastrula for zygotic gene activation58. Consistent with the data, we detected the downregulation of dnmt1 in ACs upon activin A treatment (Supplementary Tables S1 and S2). Taken together, these data imply that the in vitro culture system using ACs treated with activin A recapitulates zygotic gene activation controlled by epigenetic mechanisms in normal development.
We also newly found that socs3 was upregulated by activin A treatment (Table 1 and Fig. 5). socs3 plays a role in skin inflammation59, and it has been reported to control the balance between stem cell pluripotency and differentiation into the primitive endoderm lineage via FGF-MAPK pathway60,61. In the inferred network, a pluripotency factor, pou5f3.2.S, an endoderm marker, gata4.S, and a downstream kinase of MAPK, map3k1.S, were obtained as potential socs3-associated genes (Supplementary Fig. S6). These previous findings with the predicted socs3 subnetwork indicated that socs3 is involved in the maintenance of ACs pluripotency and activin A-driven differentiation. Further research is needed to elucidate whether activin signaling regulates the pluripotent and differentiation states in Xenopus ACs via the modulation of socs3 expression.
Taken together, our results demonstrating the developmental potency of ACs relied on activin A provide information required for early development. Our transcriptome in a mesendoderm-biased environment may serve as a resource for understanding the network during primary germ layer formation in combination with in vivo transcriptome datasets23,24,25 and previous GRN resources62,63,64,65. The in vitro ACs assay has some limitations, such as fewer cells and limiting growth factors at local levels, but it enables to understand features that could not be captured in complex processes within embryos.
Methods
Preparation of Xenopus embryos
The wildtype X. laevis strains were maintained in accordance with guidelines on animal housing66. All animal procedures were approved by the Animal Experimentation Committee of the Teikyo University (approval numbers: 18-020), and authors carried out all methods in our experiments in accordance with ARRIVE guidelines. Embryos were artificially fertilized, dejellied, and incubated in 0.1 × Steinberg’s solution67. Embryos were cultured until the blastula stages (stages 8.5–9) according to the normal table of Nieuwkoop and Faber68. The vitelline membrane was removed from embryos using forceps.
ACs dissection
ACs were removed from blastula stage embryos (stages 8.5–9) using tungsten needles, as previously described69. Dissected ACs were cultured in activin A solution (50 ng/mL in 0.5 × Steinberg’s solution containing 0.1% BSA and 0.1 mg/mL kanamycin sulfate) at 18℃. As an activin A-untreated sample, ACs were collected immediately after dissection in the absence of activin A treatment. Authors carried out all methods in our experiments in accordance with relevant guidelines and regulations.
Total RNA extraction, RNA library construction, and RNA-sequencing (RNA-seq)
Ten ACs per sample, in triplicate, were homogenized in ISOGEN (Nippon Gene). Total RNA was extracted according to the manufacturer's protocol and was purified using the RNeasy Mini kit (QIAGEN). For ethanol precipitation, the extracted RNA was mixed with ethanol and NaCl and then centrifuged at 4 °C for 30 min. After removal of supernatants, RNA pellet was washed with 80% ethanol, and was dried up and resuspended with RNase free water. RNA libraries were prepared for sequencing using standard Illumina protocols, and RNA sequencing were performed as described previously70. Reads were mapped to the X. laevis v9.2 genome (Xenbase, http://www.xenbase.org/entry/). All RNA-seq datasets are deposited in the NCBI GEO under accession number GSE153925.
Identification of differentially expressed genes (DEGs)
For comparative analysis of gene expression between Pre_activin and each Post_activin sample, read counts were normalized by calculating the number of reads per kilobase per million (RPKM) for each transcript in individual samples using the CLC Genomics Workbench software version 12.0 (QIAGEN, CLC-LICENSE-1KPRB-BMN2G-24G14-CGCSY-6CQ28). The RPKM values for each gene are listed in Supplementary Table S7. DEGs between Pre_activin and Post_activin samples were filtered at cut-off of a false discovery rate (FDR) < 0.05 and a log2 fold change (FC) ≥ 2 or ≤ − 2. The comparison of distinct gene expression patterns was visualized in a principal component analysis (PCA), heatmaps, and volcano plots using the CLC Genomic Workbench software 12.0 (QIAGEN, CLC-LICENSE-1KPRB-BMN2G-24G14-CGCSY-6CQ28).
Reverse transcription PCR (RT-PCR) assay
Equal amounts of total RNA extracted from each sample were subjected to RT-PCR as described previously71. PCR was performed using an EmeraldAmp PCR Master kit (Takara) using the following protocol: 28 cycles of 98 °C for 10 s, 55 °C for 30 s, and 72 °C for 30 s, followed by final elongation at 72 °C for 10 min. The sequences of the tested primers are listed in Supplementary Table S8. Electrophoresis with 1% agarose gel was carried out to verify the band size of the PCR products. For semiquantitative analysis, the band intensity of PCR products was quantified using Bio Image Intelligent Quantifier software (Bio Image Systems, Inc.) and the relative expression of the tested genes was calculated by dividing the band intensity of the tested genes with that of eef1α1 as an internal control. Three pools in each sample were assayed for RT-PCR. Experiments were carried out at least twice, and a representative result is shown when similar results were obtained.
Functional enrichment analysis and the inferred network
Gene ontology (GO) analysis and PPI network analysis were performed after conversion of the X. laevis genes to the human ortholog genes as previously described72. GO analysis and KEGG pathway analysis (Supplementary Table S4) of DEGs were performed using online ToppGene Suite (http://toppgene.cchmc.org). K-means clustering of DEGs was performed and visualized using iDEP.93 (integrated Differential Expression and Pathway analysis) online tools (http://bioinformatics.sdstate.edu/idep/)73. The PPI network was extracted from the human STRING network (version 11.0) and visualized using Cytoscape software (version 3.8.2, https://cytoscape.org). PPI functional enrichment was performed using String Enrichment plugin in Cytoscape74. The gene network was inferred from the temporal gene expression profiles (transformed gene expression in Pre_activin, Post 1 h, 3 h, 6 h, and Post 9h_activin ACs) of upregulated DEGs in Post 3h_ACs (4149 genes) using the Algorithm for the Reconstruction of Accurate Cellular Networks (ARACNE)75,76 plugin tool in Cytoscape. The mutual information (MI) value was over 0.8 for the inferred socs3.S subnetwork.
Use of published datasets
The dorsal marginal zone (DMZ)-enriched genes were recovered from Kakebeen et al.23 with an additional step to indicate the enrichment of DMZ at stage 11: log transformed counts (DMZ)—log transformed counts (VMZ) > 0.5. The DMZ- and ventral marginal zone (VMZ)-enriched genes were referred to Ding et al.24. The gene expression datasets in the whole embryos were referred to Session et al.25. Smad2/3-associated genes were recovered from stage 10.5 peak datasets in Gupta et al.45, and Smad2/3 and FoxhI target genes from Chiu et al.46.
Data availability
RNA-seq data sets reported in this paper were deposited in the NCBI Gene Expression Omnibus (GEO) database (www.ncbi.nlm.nih.gov/geo; accession number is GSE153925).
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Acknowledgements
We thank Dr. Akiko Kondo for critical reading the manuscript. This work was supported by the Japan Society for the Promotion of Science KAKENHI Grant Numbers 19K16147 (Y.S.-K.), and the Cooperative Research Project Program of Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA Center), University of Tsukuba, Japan.
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Y.S.-K. performed most experiments and analyzed the data. J.K. and A.F. performed RNA-seq and analyzed transcriptomic profiling. Y.S.-K. and M.A. designed the experiments and wrote the manuscript. J.K., A.F., and M.A. reviewed and edited the manuscript.
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Satou-Kobayashi, Y., Kim, JD., Fukamizu, A. et al. Temporal transcriptomic profiling reveals dynamic changes in gene expression of Xenopus animal cap upon activin treatment. Sci Rep 11, 14537 (2021). https://doi.org/10.1038/s41598-021-93524-x
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DOI: https://doi.org/10.1038/s41598-021-93524-x
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