Abstract
We studied the biodiversity of Asterochloris photobionts found in Bolivian lichens to better understand their global spatial distribution and adaptation strategies in the context of a worldwide phylogeny of the genus. Based on nuclear ITS rDNA, the chloroplast rbcL gene and the actin type I gene we reconstructed a phylogenetic tree that recovered nine new Asterochloris lineages, while 32 Bolivian photobiont samples were assigned to 12 previously recognized Asterochloris lineages. We also show that some previously discovered Asterochloris photobiont species and lineages may occur in a broader spectrum of climatic conditions, and mycobiont species and photobionts may show different preferences along an altitude gradient. To reveal general patterns of of mycobiont specificity towards the photobiont in Asterochloris, we tested the influence of climate, altitude, geographical distance and effects of symbiotic partner (mycobiont) at the species level of three genera of lichen forming fungi: Stereocaulon, Cladonia and Lepraria. Further, we compared the specificity of mycobionts towards Asterochloris photobionts in cosmopolitan, Neotropical, and Pantropical lichen forming fungi. Interestingly, cosmopolitan species showed the lowest specificity to their photobionts, but also the lowest haplotype diversity. Neotropical and Paleotropical mycobionts, however, were more specific.
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Introduction
Lichens are a marvelous example of ubiquitous, symbiotic fungi. Their thalli contain eukaryotic green algae and/or cyanobacteria, which represent the photosynthetic partners and are called photobionts; however, numerous bacteria and fungi also occur in the lichen symbiosis1,2,3. Many lichens are widely distributed, but it seems that photobionts in lichen symbioses may show their own habitat preferences independent of the lichenized fungus itself4,5,6. Since the organisms’ environmental preferences may be closely related to their distribution, the geographical patterns of the photobionts may be different from their fungal partners5. Some phylogenetic lineages, species or OTUs (operational taxonomic units), of photobionts are globally distributed, but many photobionts have only been recorded in a specific region or habitat5,7,8,9. In many cases, due to the uneven sampling of the lichen symbionts tested, it is too early to precisely define biogeographic patterns for lichenized algae; this especially applies to photobionts from the tropics5,7.
Asterochloris is one of the most common photobionts in lichen symbioses, but it is mostly restricted to certain phylogenetic lichen groups, of which undoubtedly the best sampled and studied are these associated with members of Cladonia, Lepraria and Stereocaulon4,5,9,10,11,12,13,14,15,16,17,18. So far, eighteen species have been identified within Asterochloris13,14,19,20,21,22. Nevertheless, recent research5,9,14,22,23 demonstrates there are many phylogenetic Asterochloris lineages that represent still unrecognized species, which, apart from differences in the molecular markers, also differ in climate and substrate preferences. Moreover, Škaloud et al.14 indicated that genetic diversity, ecology, biogeography and specificity to mycobiont partners should all be taken into account in species delimitation. In addition, it has been revealed that some lichens usually associated with Asterochloris spp. may have additional photobionts in their thalli, e.g., Chloroidium9 or Vulcanochloris9,16.
To date, many analyses have been carried out to determine the distribution patterns of lichenized algae, and many authors name climate as the most important factor shaping their distribution4,5,24,25,26. In addition, photobiont lineages specific to a particular geographic region have been reported24,25, but the exact factors driving photobiont selection remain unexplored. Some studies have indicated that the mycobiont can be highly specific to its photobiont7,23,27,28,29,30,31; however, mycobiont generalists (sensu Yahr et al.32, i.e. with low photobiont specificity) are also common, especially in lichens that have wide geographical and ecological ranges33,34,35. In general, photobiont selection is driven by phylogenetic specialization, mycobiont reproductive strategy, availability of motile and airborne photobiont cells, as well as key ecological factors for both symbionts32,33,36,37,38,39,40.
Despite extensive research efforts, there are many OTUs that are still unnamed. Few studies have been performed using the material from the Neotropics23,41, and from this region most Asterochloris photobionts are of unknown taxonomic affiliation9,12,23. In the present study we focused on Asterochloris biodiversity in lichen symbioses in Bolivian Andean vegetation to better understand their global spatial distribution.
Bolivia represents the geographical and geological synthesis of South America its geology is a cross-section of all geological eras42. The eastern Andean foothills have been identified as the most biologically diverse region of the country. Humidity, rainfall and temperature are important for many biological processes that led to a high speciation degree and endemism. The Yungas forests are considered the center of diversity for plants dependent on the humid seasonal climate42; this is potentially true for lichens as well. This makes Yungas forests the most important center of endemism in Bolivia42,43.
The main aim of this work is to examine the genetic diversity of Asterochloris photobionts in lichens from Bolivia and to reconstruct their phylogenetic relationships. Additionally, to better understand global spatial distribution and adaptation strategies of Asterochloris algae, we studied the role of various factors: mycobiont host, altitude, climatic conditions, and location. For some lineages, we also assessed the level of selectivity of bionts. Additionally, we present the first record of Vulcanochloris from the Neotropics.
Results
Phylogenetic analyses
In the present study we generated 54 new ITS rDNA sequences, 29 sequences of the chloroplast rbcL gene fragment, and 14 of the actin type I of Asterochloris algae. In addition, we obtained one ITS rDNA sequence of genus Vulcanochloris (Table S1). The Bayesian phylogenetic tree inferred the genus Asterochloris to be divided into two major clades (Fig. 1), similarly to the results of Vančurová et al.17. The first major clade (PP = 0.94, ML = –/84) consists of four poorly supported subclades and a single lineage of A. excentrica, although in Vančurová et al. only two subclades were found17. The second clade (PP = 0.94, ML = –/84) consists of two subclades. Among them, five lineages represent previously described species: Asterochloris erici, A. glomerata, A. irregularis, A. magna and A. pseudoirregularis; an additional four undescribed lineages also occur in this clade. Altogether we recovered 61 lineages (Fig. 1), 52 of which have been previously identified4,5,9,11,12,13,15,16,22,23, and for those we applied the same nomenclature as in previous papers. 32 Asterochloris samples obtained from Bolivian specimens grouped within 12 of these previously recognized clades, of which only two lineages were ascribed to the species level14,20 (Fig. 1). The remaining nine photobiont lineages (marked as Bol 1–9) consisted only of Bolivian samples and formed new lineages of Asterochloris, probably representing undescribed species, as shown in the ABGD analysis.
Majority-rule consensus tree from Bayesian analysis of Asterochloris based on ITS rDNA, rbcL and actin type I locus data set with posterior probabilities and bootstrap support values from RaxML and IQ-TREE analysis presented near the branches. For each record GenBank accession no. or voucher no. (for newly sequenced samples) are followed with photobiont name (if known), their mycobiont host name and the origin of specimen together with altitude (if known). Newly sequenced photobionts from Bolivia are marked in bold. Species or phylogenetic lineages are marked in boxes with appropriate names. Based on altitude of the samples, which belongs to particular phylogenetic lineage, we added summary of abundance for each lineage. Samples from 0 to 500 m a.s.l. are in first box, from 501 to 1000 are in second box, from 1001 to 1500 are in third box, from 1501 to 2000 are in fourth box, from 2001 to 2500 are in fifth box, from 2501 to 3000 are in sixth box, from 3001 to 3500 are in seventh box, from 3501 to 4000 are in eighth box, from 4001 to 4500 are in ninth box, from 4501 to 5000 are in tenth box and samples with missing data of altitude are in eleventh box, marked in grey.
Furthermore, in 12 localities from Bolivia presented in this study and two previously reported additional Bolivian localities23, we found diverse Asterochloris lineages. In locality Chuquisaca 2 (N = 7; for locality codes see Table S1), we found six different Asterochloris photobionts; two were detected for the first time. Additionally, each species of Cladonia and Lepraria from this locality was found to be associated with a different Asterochloris lineage. In locality Cochabamba 1 (N = 7), we identified seven photobionts lineages: A. mediterranea, A. friedlii, Asterochloris spp. StA1, clades 12, P2, Bol2 and Bol3, each associated with a different lichen species. Furthermore, we identified the same ITS rDNA haplotype belonging to Asterochloris sp. clade Bol1 in Lepraria impossibilis in a range of altitudes from 1900 to 2149 m a.s.l. (Table S14). Similarly, photobionts belonging to clade Bol2 from L. congesta and L. aff. congesta were collected from 3780 to 4780 m a.s.l. Asterochloris sp. clade MN082 was recorded in localities Chuquisaca 2, La Paz 1, Santa Cruz 1, Tarija 1 and 2, within thalli of Neotropical (Cladonia aff. ahtii, C. ceratophylla) or Pantropical (Lepraria sipmaniana) lichens in a range of altitudes from 980 to 2750 m a.s.l., while Asterochloris sp. clade Bol1 associates with Lepraria impossibilis from localities Tarija 1 and 2, and with L. harrisiana from locality Chuquisaca 2. Asterochloris sp. clade P2 was found in Pilophorus cf. cereolus from Costa Rica, and also in eight Bolivian localities in altitudes between 2237 and 3860 m a.s.l. in Cladonia spp., Cladia aggregata and Stereocaulon myriocarpum (Fig. 1; Tables S1, S2).
We also observed dissimilarities in photobionts composition within four habitat types: lower montane cloud forest, upper montane cloud forest section 1, upper montane cloud forest section 2 and open high Andean vegetation (Figs. S6, S7). For example, in lower montane forest (N = 12), Asterochloris spp. clades Bol1, Bol3, Bol4, A14, 9 and MN082 were present, while in the first section of upper montane cloud forest (N = 26), Asterochloris spp. clades 9, A14, L54, P2, S1, MN082 and Bol1, Bol5, Bol6, Bol8 and Bol9, A. mediterranea and A. friedlii occurred. In addition, in the second section of upper montane cloud forest (N = 28), Asterochloris spp. clades 9, P2, S1, 12, A14, StA1, Bol2, A. medirerranea, A. friedlii and Vulcanochloris sp. were detected, while in the open high Andean vegetation (N = 11), Asterochloris spp. clades 8, StA1, StA5, A6, Bol2 and Bol7 were present. This shows that diverse photobiont types may occur within a steep gradient of altitude. With increasing altitude above sea level, the average temperature and amount of precipitation diminish (Fig. S7). This means that photobionts in the open high Andean vegetation should be resistant to low temperatures and periods of drought, and species of photobionts in the lower montane cloud forest should be resistant to higher temperatures and its seasonal changes. Humidity (as seasonality of rainfall) in Stereocaulon has already been indicated as a determining factor for Asterochloris, Vulcanochloris and Chloroidium9.
Based on the observed differences in composition of photobionts of four habitat types in Bolivia, we concluded that some previously known Asterochloris lineages may occur in a broader spectrum of climatic conditions (data summarized in Table S9 and in Fig. 1 in reference to altitude). Thus, A. mediterranea only known from the Mediterranean, temperate Europe and islands of the North Atlantic and Indian Oceans can also occur in the Neotropics. Annual precipitation in areas in which this species occurs ranges between 18 and 974 mm, with precipitation in the driest quarter between 2 and 95 mm (Fig. 2). Asterochloris sp. OTU2523 was found on islands of the North Atlantic, Indian and Pacific Oceans. We also identified this particular lineage in Bolivia in Cladonia ceratophylla, C. aff. ahtii and Lepraria sipmaniana. Our phylogenetic analysis revealed that this lineage is similar to a photobiont from Cladonia didyma (clade MN082) from USA31 and we conclude it may possess tolerance to a wide range of climatic condition. We do not have complete data for all specimens, but our rough estimate for annual precipitation for this lineage ranges between 591 and 4930 mm, with precipitation of the driest quarter between 11 and 1009 mm. Asterochloris friedlii was previously known from temperate Europe and USA as well as tropical areas of China and Korea5,12, and we found it in a Bolivian sample of the widespread Lepraria finkii. A similar situation was observed for Asterochloris sp. clade StA5, which to date has been found to associate with Stereocaulon spp.9. Likewise, Asterochloris sp. clade 9 found in South America (this study), India and Southern North America is associated only with Cladonia spp.5,18,23,32,41; based on our analysis, this lineage shows tolerance for a broad annual mean temperature range (− 5.2–24.9 °C) and is characterized by drought resistance and rehydration (annual precipitation: 591–4930 mm, precipitation of driest quarter: 8–1009 mm, precipitation of wettest quarter: 143–3968 mm). The widespread lineage Asterochloris sp. clade 12 (also found in this study) associates with Cladonia spp. and Stereocaulon spp.5,9,11,13,23 and is characterized by the widest annual temperature range we have seen: − 16.9–18.1 °C and high drought resistance (precipitation of driest quarter: 10–165 mm). Asterochloris sp. clade P2 was found to associate with different lichen species and genera (Cladia, Cladonia, Pilophorus and Stereocaulon) [this study11,23]. This lineage, together with closely related Asterochloris spp. clades L54 and S1, were all found only in the Neotropics [this study11,23]. So far, Asterochloris spp. clades L54 and A6 have been found to form a symbiosis exclusively with Lepraria spp. (Fig. 1) [this study4,11].
Box-plot diagram representing differences in climatic preferences for selected species and lineages of Asterochloris photobionts. Climatic data were obtained from the Global Climate Data—WorldClim. BIO1 = Annual Mean Temperature (°C), BIO7 = Temperature Annual Range (°C), BIO12 = Annual Precipitation (mm), BIO17 = Precipitation of Driest Quarter (mm).
Statistical analyses
To identify the impact of selected factors on the distribution of Asterochloris photobionts, we performed variation partitioning analyses (Tables S7, S8) that showed that our selected variables explained 46–47% of the variation for Bolivian samples. 25–26% of the variation was explained by the species of the mycobiont (Table S7), which shows low correlation between photobiont distribution and mycobiont hosts. 22–23% was explained by environmental factors. 5% of the variability was shared between mycobiont host, climate and altitude or habitat type, while, 6% of the variability was explained by climate as independent factor.
In the case of Stereocaulon, 39% of the variation was explained by our selected variables. The largest part of the variation in diversity of photobionts was explained by the mycobiont hosts (12%, Table S7). As independent factors, altitude explained 8% of the variation, while geographical distance—explained 4%. For Cladonia spp., 58% of the variability was explained by our selected variables (Table S7). 31% was explained by mycobiont species, whereas climate and geographical distance explained 5% and 3%, respectively. However, ecological factors shared 11% of the variability. In the case of Lepraria spp., altitude appeared to be insignificant (Table S6). Results of PCoA analyses for Lepraria spp. show moderate correlation between photobiont distribution and mycobiont host (35% of the variation explained by mycobiont). The remaining factors did not exert significant influence.
In addition, we selected 12 cosmopolitan, 17 Neotropical and ten Pantropical species from our dataset, and supplemented by data available in GenBank (Table S5). In the case of cosmopolitan species, variation partitioning analyses revealed that 0–13% of the variability was explained by mycobiont hosts (Table S8), climate accounted 15% of the variability; altitude and substrate appeared to be insignificant (dbRDA analyses; Table S6). Overall, 59% of the variability of Asterochloris distribution in Neotropical and Pantropical lichens can be explained by the mycobiont hosts. We performed PCA analyses to identify which photobionts were represented by selected groups of lichens, as a function of climate (Fig. S2). PCA ordination of all analyzed species resulted in 72.7% cumulative variance explained on the first 2 axes (first—47.8%, second—24.9%). Lichen groups and their photobionts, respectively, showed indifference relative to climatic parameters: i.e., they are scattered across the hyperdimensional climatic space (Fig. S2). However, cosmopolitan, Neotropical and Pantropical species as groups represent different range of climatic conditions. In the case of cosmopolitan species, the total explained variation in the biplot was 68.4% by component 1 and 2 (Fig. S3). PCA ordination of Neotropical species, on the other hand resulted in 84.6% (Fig. S4) cumulative variance explained on the first 2 axes; and for Pantropical species it was 78.7% (Fig. S5).
However, in cosmopolitan, Neotropical and Pantropical groups of lichens (and also between them), different genera show indifferences, dependent on diverse climatic conditions (potentially climatic preferences). Tropical species prefer higher temperatures in warm and wet seasons, while the cosmopolitan species are adapted to extreme temperatures (BIO4, 7).
Due to the dissimilarities we observed in the photobionts composition of the four habitat types in Bolivia, we performed PCA to visualize variability of photobionts of those habitat types in the context of climatic factors (Fig. S6). This shows that different conditions prevail in each habitat, however, the ranges of photobionts from different habitats may overlap.
Haplotypes
Due to differences in the impact of the mycobiont species on the distribution of photobionts in selected genera of lichens, as well as differences in tolerance for climatic conditions, we performed ITS rDNA haplotype analyses to study the associations of mycobiont hosts and photobionts in selected groups of lichens. In the case of Asterochloris photobionts from 200 Stereocaulon samples (Table S10), we found low haplotype diversity (0.28) and detected 55 photobiont ITS rDNA haplotypes (Tables S10, S16), of which 24 were identified in samples from tropics, 36 in samples from the temperate zone, and 5 from both regions. However, haplotype diversity for tropical samples appeared to be higher (0.52) than that from temperate areas (0.24) (Table S17). Asterochloris irregularis appeared to be the most common photobiont associated with Stereocaulon spp. in temperate climates (62 samples). In Stereocaulon samples from Bolivia, we found five distinct haplotypes representing A. mediterranea, Asterochloris spp. clades P2, S1, and Bol4 and Bol7. Moreover, some species showed a high flexibility in their photobiont choice, and furthermore, it appears their photobiont pools may differ depending on the climate, e.g. in Stereocaulon vesuvianum we found haplotypes belonging to Asterochloris glomerata, A. irregularis, A. pseudoirregularis, Asterochloris spp. clades A9, StA1 and StA7 in temperate areas, while Asterochloris spp. clades StA3, StA5, T20 and A. mediterranea (Table S10) in tropical regions.
In the case of Asterochloris associated with Cladonia spp., we found 268 ITS rDNA haplotypes (haplotype diversity = 0.42) based on 630 samples (Tables S11, S16). 132 originated from tropical regions, 143 from temperate areas and only 10 Asterochloris haplotypes were noted in both regions. Moreover, we observed differences in photobiont haplotype diversity within the tropical and temperate Cladonia samples (tropical—0.63, temperate—0.35). The most common photobiont in Cladonia spp. from tropical areas seems to be Asterochloris sp. clade 9 (71 samples); this potentially new species is represented by 54 haplotypes in our dataset. Moreover, samples of this clade were also found in 13 Cladonia specimens from temperate regions. Bolivian specimens of Cladonia spp. were found to associate with 34 haplotypes belonging to A. mediterranea, Asterochloris spp. clades 9, 12, A14, S1, StA1, StA5, P2, MN082, Bol3 and Bol5.
Photobionts from Lepraria spp. (Tables S12, S16), represented by 44 haplotypes of Asterochloris, showed high haplotype diversity (0.54). In tropical climates 14 haplotypes were found, while in temperate areas we found 30 haplotypes. The most common photobiont in Lepraria spp. is a haplotype of A. friedlii (14 sequences from both regions). Moreover, haplotype diversity was higher in tropical (0.70) than temperate (0.49) regions (Table S17).
In the case of lichens representing cosmopolitan distribution patterns, we observed 62 Asterochloris haplotypes (Table S13) with high haplotype diversity (0.53) (Table S16). These haplotypes belong to 30 Asterochloris lineages. Samples from tropical climates were found to associate with 14 lineages, while specimens from temperate climates associated with 21. Only one was found in both regions (haplotype 28 in Table S13). Some lichen species appear to adopt different Asterochloris haplotypes in different climatic regions, e.g. Cladonia furcata associates with Asterochloris spp. clades 12 or 8 in temperate areas, while other haplotypes were found in tropical areas (clades I1 and I2 in India, and clades P2 and Bol5 in Bolivia). Remarkably, Stereocaulon alpinum appears to associate with at least 11 different Asterochloris lineages, of which clade Bol7 and A. lobophora have only been found in tropical climates. Interestingly, Asterochloris sp. clade StA5 was found both in tropical and temperate regions: Bolivia, Austria (one haplotype) and in Canada (a different haplotype). Additionally, Stereocaulon alpinum collected in temperate regions was also found to associate with A. irregularis, A. italiana, A. phycobiontica, A. woessiae and Asterochloris spp. clades StA2, StA4 and A9 (Table S10). In the case of Cladonia pyxidata, it was found to associate with haplotypes of A. glomerata, A. lobophora, and Asterochloris sp. clade12 in temperate regions; with A. sejongensis in Antarctica; and with Asterochloris spp. clades 12 and I1 in tropical regions. Indeed, Asterochloris sp. clade 12 may associate with this species in both regions (Table S11). Seventeen Neotropical lichen species were analyzed; among them we identified 29 haplotypes of Asterochloris belonging to 16 lineages (Table S14) with high haplotype diversity (0.83) (Table S16). In the case of the Pantropical group, ten lichen species associated with 32 Asterochloris haplotypes belonging to 13 lineages (Table S15) and showed high haplotype diversity (0.80) (Table S16). Within Neotropical and Pantropical lichens, we found multiple Asterochloris haplotypes either restricted to a single species or with potentially wide selectivity (i.e., occurring in different species in similar or diverse localities).
Discussion
Little is still known about the nature of the associations between mycobionts and photobionts. Only several works have explored the diversity of Asterochloris photobionts in the tropics5,9,11,12,18,31,32,41. In this study, we recovered nine new Asterochloris lineages, while 29 Bolivian photobiont samples were assigned to 12 previously recognized Asterochloris lineages, from which only two have been so far formally described (Fig. 1).
In this study we also showed that some previously known Asterochloris photobiont lineages may occur in a broader spectrum of climatic conditions, e.g. A. mediterranea is here reported for the first time from Neotropics. On the other hand, alpine and psychrophilic Asterochloris sp. clade StA59 may occur in open high Andean vegetation and may show higher drought resistance (Precipitation of Driest Quarter for sample UGDA-L 18963 = 30 mm) than previously thought (80–341 mm) (Fig. 2). Asterochloris spp. clades P2, A14 and L54 are considered Neotropical lineages, as well as clades Bol1 and Bol2 recovered in this study, neither of which is closely related to previously described lineages.
Preferences of mycobionts for certain types of photobionts have been repeatedly recognized for Trebouxia8,29,44, Asterochloris9,14, or Trentepohliaceae7. The photobiont’s ecological specialization probably determines the mycobiont’s selection for its symbiont4,44,45. Furthermore, horizontal transmission has probably increased the taxonomic range of compatible photobiont partners15; for example Vančurová et al.9 showed that several algal species or lineages show specificity towards a single mycobiont species. On the other hand, they also recovered lineages that were not specific towards a single mycobiont. Our data shows that previously described Asterochloris lineages can associate with broader ranges of mycobionts. For example, Asterochloris spp. clades StA1 and StA5, were previously found only within Stereocaulon spp., but we found them associated with different Cladonia species, suggesting a lower level of selectivity and specificity (sensu Beck et al.10). Interestingly, Asterochloris sp. clade MN082 was found in samples of L. sipmaniana, Cladonia aff. ahtii, and C. ceratophylla from different localities ranging in altitude from 980 to 2750 m a.s.l; these results are preliminary, however, further analyses are necessary to determine the exact selectivity level of these algae.
Ecological factors may modulate the availability of photobiont species/strains at various sites, thus reducing the number of possible associations in isolated populations. The influence of altitude gradient on photobiont population structure was previously described7,46,47,48. In our study, we found some changes in photobiont populations along altitude gradients; e.g., Asterochloris spp. clades Bol1 and MN082 were present in lower montane cloud forest and upper montane cloud forest section 1; in upper montane cloud forest section 1 and section 2 were Asterochloris spp. clade P2 and S1, as well as A. mediterranea and A. friedlii. In each altitude dependent habitat, unique Asterochloris lineages occur. We need more data to define exact ecological niche for those lineages.
To reveal global patterns of specificity of the mycobionts towards the photobiont in Asterochloris, we tested the influence of climate, altitude, geographical distance and effects of the symbiotic partner (mycobiont) at the species level for three genera of lichen forming fungi: Stereocaulon, Cladonia and Lepraria. Stereocaulon species are widespread and have broad ecological requirements9, and associate with many Asterochloris species [14, this study], but also Chloroidium9,10 and Vulcanochloris [16, this study]. The diversity of photobionts in Stereocaulon and the association between their diversity and environmental conditions was previously conducted on a global scale by Vančurová et al.16. The photobionts distribution in Stereocaulon species found in that study appeared to follow a pattern that was highly influenced by substrate type. However, in that study this was mostly evident in Chloroidium and Vulcanochloris. Our analyses confirmed the hypothesis that photobiont variability may be dependent on geographical distance32. In the case of Stereocaulon9, 4% of the photobiont variability was explained by a net effect of geographical distance. Furthermore, the distribution of photobiont diversity in Stereocaulon may also be affected by altitude (8%). It has been previously shown that the majority of Stereocaulon mycobiont species appear to be specific towards phycobionts9,17. In our study, Stereocaulon displayed a low level of selectivity toward photobiont lineages (Table S10). Furthermore, we report low haplotype diversity for Asterochloris photobionts of Stereocaulon that can be correlated with oversampled photobionts from temperate regions.
Yahr et al.32 demonstrated that geographic position and habitat are the best predictors of algal genotype distribution in Cladonia. Steinová et al.15 showed that photobionts are significantly structured by climate and geography, but the mode of reproduction was revealed to have the greatest impact on Cladonia photobiont diversity. In our analyses we found that 36% of the variability was explained by mycobiont at the species level. Furthermore, we report higher haplotype diversity in photobionts from Cladonia spp. than Stereocaulon spp., in which the predominating photobiont species was Asterochloris glomerata (21 haplotypes represented by 104 sequences; the most common haplotype represented by 51 sequences). Nonetheless, Cladonia and Lepraria are known to associate with a wide range of Asterochloris species4,14. With regards to Lepraria, we found that these mycobiont species associated with particular Asterochloris taxa from lineages B and C (sensu Vančurová, et al.9) and (like Cladonia), show moderate selectivity toward their photobionts, with a moderate influence of mycobiont species and a very low influence of climate and spatial structure. In addition, species of both genera may show different ranges of adaptation strategies. These results support a hypothesis that mycobionts can associate with numerous symbiotic partners in the case of ubiquitous lichen species adapted to various ecological conditions8,13,23,24. The dominant species of Asterochloris found within Lepraria spp. was A. friedlii (found in 22 samples, 27% of tested samples). However, more research is needed to reveal the most common Asterochloris spp. in Lepraria as well as to fully explain the factors affecting photobiont distribution.
Lichens exhibit various distribution patterns at the micro- and macro-levels. In the case of lichen-forming fungi, 16 main biogeographic patterns were distinguished (including cosmopolitan, bipolar, paleotropical, Neotropical, Pantropical, Mediterranean)49, but the biogeography of lichen photobionts is still poorly known. Preferential associations with locally adapted symbionts have been reported repeatedly8,50,51. It was hypothesized that low specificity of the host towards its symbiotic partner helps the host to take advantage of the locally adapted symbiotic partners and colonize broader geographic areas8. In the case of higher specificity, the host is expected to have a narrower ecological niche, and a restricted geographical distribution. In the case of lichen symbioses, the generalist pattern is more common8,24,47; however, a specialist pattern has been reported for Nostoc-associated lichen fungi52. We compared the specificity of mycobionts towards Asterochloris photobionts in three groups of lichens: cosmopolitan, Neotropical, and Pantropical. Interestingly, cosmopolitan species repeatedly showed the lowest specificity towards photobionts, but also had the lowest haplotype photobiont diversity. More haplotypes were identified in temperate regions; however, the haplotype diversity of tropical photobiont lineages was higher. This may indicate a significant under-sampling of tropical regions. In addition, the distribution of the diversity of photobionts within cosmopolitan lichens was influenced by a moderate impact of climatic conditions (15%), while in the case of Neotropical and Pantropical lichens there was a correlation between the mycobiont species and climatic conditions. This indicates the selection of locally adapted photobionts in cosmopolitan lichens, while tropical species mostly demonstrate habitat preferences. However, in the light of the results obtained here and in previous research8,53, we assume that the selection of a symbiotic partner may be based on the search of the best-suited photobiont. To obtain more accurate results, additional data on one species commonly found in the Neotropical and Pantropical regions are needed, with emphasis on the impact of habitat conditions.
In conclusion, to further investigate these questions, we suggest that a suitable species, representative of each fungal genus associating with Asterochloris, should be selected for future studies before sampling. Furthermore, to avoid geographical gaps, sampling should be extended to the whole world. In addition, on the basis of the results obtained here, it can be assumed that Bolivia, due to its geological and ecological diversity, may reflect the biodiversity of the entire Andean region in the Neotropics, where the issue of photobionts' biodiversity still remains unresolved. Indeed, the tropics may be an important, under-explored source of hidden photobionts biodiversity.
Materials and methods
Material
54 lichens samples representing 4 genera: Cladonia, Diploschistes, Lepraria and Stereocaulon containing Asterochloris photobionts from various habitats in Bolivia were selected randomly for studying the biodiversity of Bolivian Asterochloris photobionts. Lichens were collected from various substrata (rocks, soil, tree bark, wood, and bryophytes). The majority of samples were obtained from Yungas cloud forest, but also from Tucuman-Bolivian forest and dry inter-Andean forest.
Lichen samples were collected in Bolivia with the permission of Ministerio de Media Ambiente y Agua (MMAYA/VMABCC GDF/DGBAP/MEG No 03272/2016) and in cooperation with Herbario Nacional de Bolivia (LPB), who in turn made specimens available to the herbarium of University of Gdańsk. Original samples are deposited in herbarium LPB, with duplicates stored in University of Gdańsk (UGDA). Lichen species were determined using appropriate identification keys54,55,56,57,58. Morphology, secondary chemistry, and for Lepraria spp., sequencing of nucITS rDNA of mycobiont59 were used for specimen identification. Lichen substances were identified with thin-layer chromatography (TLC) in solvents A and C60. Detailed locality data are presented in Supplementary Data Table S1.
The ranges of distribution were defined for lichen species used in this study—Cosmopolitan, Neotropical or Pantropical, based on the division proposed by Galloway49. The best sampled species were selected, and after scrutiny of the literature, a database was created for further analysis.
Molecular methods
Well-preserved specimens lacking any visible symptoms of fungal infection were used for DNA isolation (total lichen DNA) following the CTAB protocol61. For molecular identification of the photobionts (Asterochloris), the nuclear internal transcribed spacer (ITS, ITS1-5.8S-ITS2)11,31,62, the chloroplast rbcL gene and in few cases of unique lineages of Asterochloris63,64,65, and the actin type I gene were amplified10. The PCR condition are presented in Supplementary Data Table S3. Sequencing was performed in Macrogen® in Amsterdam, Netherlands using amplification primers. Sequences were compared to the sequences available in GenBank using Megablast searchers66 to verify their identity and detect potential contaminants. The newly obtained sequences of the ITS rDNA, and fragments of the rblcL gene and the actin type I regions were deposited in GenBank (Table S1).
Phylogenetic analyses
The Asterochloris sequence datasets were analyzed as a concatenated dataset of all loci. In total, 189 sequences with a total of 2618 sites were generated. The ITS rDNA dataset consisted of 188 sequences, including 54 newly obtained and 134 previously published representative sequences of taxa retrieved from GenBank. These representative sequences were selected following the latest review paper by Vančurová et al.9, as well as additional, new Asterochloris spp. described by Kim et al.22 and Pino-Bodas and Stenroos23. To avoid confusion, the nomenclature of some mycobiont species associated with Asterochloris has not been updated according to the newest taxonomical works; these are: Lepraria borealis and L. caesiolaba (both now subsumed under L. neglecta), L. lobificans auct. (now L. finkii), L. nigrocincta (now L. yunnaniana) and Parmelinopis minarum (now Hypotrachyna minarum). The chloroplast rbcL gene dataset consisted of 64 sequences (29 newly obtained sequences). The actin type I dataset consisted of 132 sequences: 14 newly obtained sequences, and previously published sequences. Sequence alignment was automatically performed using MAFFT—Multiple Alignment using Fast Fourier Transform67 as implemented in UGENE68. Phylogenetic relationships were inferred with Bayesian Inference (BI) carried out in MrBayes v.3.2.269. In cases of which rbcL or actin sequences were lacking, they were treated as missing data. Two parallel MCMC runs were performed, using four independent heated chains and 10 million generations, sampling every 1000th tree. The initial 2500 trees of each run (25%) were discarded as burn-in, and posterior probabilities were estimated by constructing a majority-rule consensus tree of all sampled post-burn-in trees. Maximum likelihood (ML) analyses were performed using RAxML-HPC v.8 with 1000 bootstrap replicates (ML-BS), the GTRGAMMAI model70 and the edge-linked partition model in IQ-TREE71,72 on the CIPRES Science Gateway73. The best-fit substitution models and block (Table S4) were selected using Akaike Information Criterion (AIC) as implemented in PartitionFinder 274. The tree topology obtained by ML method did not contradict the Bayesian tree; therefore, only the Bayesian tree is shown. The consensus trees were visualized using FigTree v1.4.275. Branches with bootstrap support ≥ 70% and posterior probabilities ≥ 0.95 were considered to be strongly supported. Analogically, an ITS rDNA dataset of Vulcanochloris, including a single new sequence of Vulcanochloris sp. detected in Stereocaulon pityryzans (UGDA-L 18522), was analyzed. It consisted of 16 sequences, of which 15 were previously published sequences were retrieved from GenBank.
OTUs delimitation
To delimit Asterochloris OTUs, the Automatic Barcode Gap Discovery method (ABGD) was used, following Leavitt et al.27. The analyses were performed using the webserver (https://bioinfo.mnhn.fr/abi/public/abgd/abgdweb.html) separately for the ITS rDNA and actin datasets. The Jukes and Cantor (JC69) model was applied to calculate the genetic distance. Pmin = 0.001, Pmax = 0.01, step = 10, Nb bins = 20 and X (relative gap width) = 0.5 were used. Next, comparisons of the inferred OTUs were made based on both markers. Affiliation to individual species or Asterochloris phylogenetic lineages used in further analyses were estimated based on published data. These data are available in Table S2. In total, 61 OTUs were inferred.
Statistical analyses
The relationship between species richness of Bolivian photobionts and lichen species, as well as the relative effects of climate, altitude, geographical distances, substrate, and habitat type, were analyzed. In the case of data obtained from GenBank, only records with precise geospatial coordinates were used. The comparative effects of selected variables were analyzed by variation partitioning in redundancy analyses, using the varpart function in the vegan package76. The phylogenetic distances of photobionts were used as a response variable, coded as the first 10 PCoA axes. Climatic data were obtained from the Global Climate Data—WorldClim Version 277 at a resolution of 2.5 arc minutes. The 19 environmental variables, altitude, substrate and habitat type were transformed into principal component variables (PCs). Principal coordinates of neighbor matrices vectors (PCNM) representing the geographical distances at various spatial scales78 were obtained from the transformation of geographical distance values (latitude and longitude). PCNM vectors were calculated based on the pairwise geographical distances obtained by the distGPS function of the BoSSA package79. Distance-based redundancy analyses (dbRDA)80 were used to select statistically significant predictors for explaining variation for each of the data-sets used in the variation partitioning analyses.
Variation partitioning analyses were carried out separately for all Bolivian data obtained in this study (N = 54) and in Pino-Bodas & Stenroos23, and also for Asterochloris from selected lichen genera, i.e. Cladonia (N = 179), Stereocaulon (N = 169) and Lepraria (N = 34) due to better sampling of these genera. We found that the substrate influence appeared to be statistically insignificant and did not correlate with our Bolivian datasets (Table S6). Furthermore, due to multiple missing data for this particular variable, we decided to omit this factor in the analyses of the datasets of the three genera. Variation partitioning analyses were also performed for selected group of lichen forming fungi representing different distribution patterns, i.e. cosmopolitan (N = 77), Neotropical (N = 30) and Pantropical (N = 23) (patterns of distribution identified based on the literature54,55,56,57,58; Table S5). Due to limited data for species showing different distribution patterns, different PCoA analyses schemes were used. A series of analyses were performed using mycobiont host as an explanatory variable constantly together with an other variable, which was replaced in subsequent series (geographical distance, altitude, substrate and climate; Table S8). We took into account only morphospecies; we did not consider the possibility of one morphospecies comprising different phylogenetic lineages (i.e.cryptic species) a situation that can potentially be geographically or ecologically more restrictive, as has been demonstrated in the Cladoniaceae81.
To detect and visualize the differential ordination (tendency or strategies) of samples in the hyperspace, we performed Principal Component Analyses (PCA) according to climatic factors (BIO1–BIO19) and the grouped results depending on the distribution patterns of lichen species and the genus of mycobiont host. In addition, we performed these analyses for Bolivian samples, grouping results by habitat type. All analyses were performed in R v 3.6.082, using RStudio v.1.2.133583.
Haplotypes
To visualize the precise interaction between mycobionts and photobionts, we inferred haplotypes from a pairwise character difference matrix of ITS rDNA sequences using the haplotype function in haplotypes package84. These analyses were carried out for six data sets (Tables S10–14) consisting of sequences newly obtained for this paper aligned with additional sequences downloaded from GenBank of Asterochloris from genera Cladonia, Stereocaulon and Lepraria. We also ran analyses for lichen-forming fungi representing different distribution patterns: cosmopolitan, Neotropical, Pantropical. Lastly, we measured nucleotide diversity, the number of haplotypes, and haplotype diversity as further estimations of differences between groups.
Data availability
DNA Sequences are deposited in GenBank: ITS rDNA MW847807-MW847861, actin type I locus MW845956- MW845969, and rbcL gene MW847862- MW847890. DNA matrix used for phylogenetic analyses of Asterochloris, as a concatenated dataset of all loci is available at TreeBASE version 2 web server85,86, http://purl.org/phylo/treebase/phylows/study/TB2:S28062.
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Acknowledgements
We greatly appreciate Adam Flakus and Pamela Rodriguez-Flakus (Polish Academy of Sciences, Kraków) for great support in the field studies. We would like to thank members of Herbario Nacional de Bolivia, Instituto de Ecología, Universidad Mayor de San Andrés La Paz, for the generous cooperation and our colleagues and all protected areas staff (http://sernap.gob.bo) for their help during the fieldwork. Scott LaGreca (Duke University) is acknowledged for language editing and proofreading. This research received support from the National Science Centre (Project No 2015/17/B/NZ8/02441: Hidden genetic diversity in sterile crustose lichens in the Neotropical forests—an innovative case study in Bolivia, a hotspot of biodiversity).
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M.K1. and B.G.-K. designed the study. M.K. conducted fieldwork, collected specimens and delimit the species of mycobionts. M.K., I.Č., and A.J. performed laboratory work with contributions from P.Š. M.K1. analyzed the data with contributions from P.Š. and B.G.-K. M.K1., M.K., B.G.-K. and P.Š. wrote the manuscript.
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Kosecka, M., Guzow-Krzemińska, B., Černajová, I. et al. New lineages of photobionts in Bolivian lichens expand our knowledge on habitat preferences and distribution of Asterochloris algae. Sci Rep 11, 8701 (2021). https://doi.org/10.1038/s41598-021-88110-0
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DOI: https://doi.org/10.1038/s41598-021-88110-0
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