Abstract
In lime concretion black soil, a two-factor (tillage and irrigation) split block experiment from 2015 to 2017 was conducted to identify whether their combination is suitable for the improvement of winter wheat yield and water use efficiency. The main treatments were subsoiling (SS) and rotary tillage (RT), with secondary treatments of three irrigation regimes: no irrigation during the whole growth period (W0), irrigation at jointing stage (W1), and irrigation at both jointing and anthesis stages (W2). In combination with a soil column experiment, the contribution of the root system in different soil layers to yield was clarified. The results indicated that both tillage and irrigation significantly influenced the spatiotemporal distributions of the root systems and yield components, while tillage produced the strongest effect. Compared with RT, SS significantly promoted the root penetration and delayed root senescence in deep soil layers. With increasing soil depth, each root configuration parameter (dry root weight density, DRWD; root length density, RLD; root surface area per unit area, RSA; root volume per unit area, RV) gradually decreased, and the peak appearance times of each root parameter in RT and three parameters (RLD, RSA and RV) in SS were postponed from heading to anthesis and from anthesis to filling stage, respectively. The average post-peak attenuation values at soil layers from 60 to 100 cm in W1 were less than those in W0 and W2. SSW1 generated the highest grain yields, with an average increase of 31.88% compared with the yield in RTW0. Root systems at three soil layers (0–40 cm, 40–80 cm and below 80 cm) differentially contributed to grain yields with 78.32%, 12.09% and 9.59%, respectively. The growth peak of the deep root system in SSW1 was postponed to the filling stage, and the post-peak attenuation declining rates were also slowed. Therefore, SSW1 is an effective cultivation method improving grain yields and water use efficiency in lime concretion black soil.
Similar content being viewed by others
Introduction
Wheat (Triticum aestivum L.), one of the most important food crops in the world, is the main source of daily protein and provides 20% calories consumed by humans1. China has the largest wheat growth areas and productions with 2.3 × 107 ha and 1.3 × 108 t in 2020, respectively. There are about 3.2 × 106 ha lime concretion black soil in China, and it accounts for 13.9% of wheat growth areas and is mainly distributed in Anhui, Henan, Shandong and Jiangsu provinces. In this soil type, wheat grain yields are lower over 15% than those in other types of soil, because its groundwater is shallow, and water shortages often occur under dry weather conditions, especially in growth seasons with strong evaporation and high water consumption2. During these water shortage periods, the rise of capillary water hardly keeps up with the loss due to evaporation and transpiration in the upper layer of the soil. Thus, the supplementary irrigation is necessary to ensure a high and stable yield. In recent years, successive RT, heavy machinery rolling, and unsuitable land preparation operations have caused changes in the soil structure of most wheat fields, especially those with lime concretion black soil3,4. With such operations, the plough layer becomes shallower, the plough pan is thickened and moved upward, soil compaction and bulk density are increased, and the transduction conditions of water, vapour and heat are destroyed5. Moreover, soil compaction stress and water conduction obstruction have become important issues affecting wheat root growth, yield formation and the improvement of water and fertilizer utilization efficiency. In addition, the conditions of field irrigation facilities in this region are relatively bad, and most of farmers neglect irrigation. In lime concretion black soil, therefore, agronomic measures such as tillage and irrigation can effectively improve the physical properties, reduce the imbalance between the soil water storage and supply, and create a soil environment conducive to wheat root growth and high efficient water utilization to achieve simultaneous improvement in wheat grain yield and water use efficiency. These are key measurements to improve the comprehensive production performance of winter wheat in this region.
In lime concretion black soil, the composition, structure and porosity of lime concretion black soil are very different from other soils, which cause this soil to have a low water-holding capacity, weak hydraulic conductivity, slow capillary water rising speed and a small rising height2. Lime concretion black soil is characterized with undesirable properties, such as high viscosity, dry shrinkage, wet expansion, poor soil structure and short suitable tillage time, which have become the main factors limiting further increases in winter wheat yield6.
Previous studies have shown that compared with traditional cultivation, optimized cultivation improves the wheat root distribution, delays wheat root senescence, and promotes the absorption of water and nitrogen in deep soil, all of which increase wheat production7. Strip RT after SS is a high-yield, efficient and water-saving tillage practice that can increase farmland water consumption, enhance stored soil water consumption, promote dry matter accumulation and increase the photosynthetic rate and water use efficiency of flag leaves8,9,10. In addition, once or twice suitable irrigations are beneficial to obtain higher yield and water use efficiency11, and the irrigation water use efficiency is highest when irrigation is operated at the critical period of water12. The CERES-Wheat model can simulate and predict the effects of different irrigation modes. Studies have shown that the application of 75 mm of irrigation water during jointing and anthesis is the best irrigation strategy in the North China Plain, and it is believed that one irrigation event at the jointing stage can be used as an alternative irrigation mode to reduce irrigation and maintain the sustainable development of farmland water resources13. In semi-arid areas, the ridge-furrow rainfall harvesting system combined with 75 mm of irrigation increases soil moisture across the rooting area, making it a high-yield and efficient water-saving strategy14.
In previous studies, the effects of single factor, such as variety15, planting density16,17,18, cultivation pattern19, fertilizer application20,21, irrigation amount, and irrigation frequency22, on root growth and wheat development were examined. The researches about irrigation-tillage mostly focused on soil properties23, greenhouse gas emissions24, water use efficiency and yield6. Gajri and his colleagues showed that deep tillage and early irrigation could short the time needed for root to reach a specified depth, thus improving water use efficiency and achieving high yield25. For lime concretion black soil, it has been shown that excessive irrigation is not conducive to the efficient use of water and the combination of SS and one irrigation event at jointing is a suitable cultivation pattern that results in simultaneous increases in winter wheat yield and water use efficiency in this region6. However, the regulatory effects of tillage and irrigation on the root parameters of winter wheat remain unclear in different soil layers in lime concretion black soil. In this study, tillage, irrigation, and their interaction were implemented in the field conditions of the lime concretion black soil for two wheat growth seasons. To evaluate the contribution of roots in different soil layers to grain yields, in addition, soil column experiment was also conducted. The objective of this study was to explore the effects of tillage and irrigation on root characteristics and grain yields in lime concretion black soil, and clarify their regulatory mechanisms underlying the suitable wheat root construction in this soil type.
Results
Effects of tillage and irrigation on yield
As shown in Table 1, the significant effects of tillage and irrigation on grain yield and its components during the wheat growing season were similar between the two years. Grain yield and its components in SS were higher than those in the RT treatment, except the kernel number per spike in 2016–2017. However, in both tillage treatments, the yield in W1 was the highest, and that in W0 was the lowest. The average yield in W1 was 22.18% and 24.16% higher than that in W0 under SS and RT, respectively. Moreover, the yield in SSW1 was the highest in the two years, with an average increase of 31.88% compared with the lowest value, which was observed in treatment RTW0. Under a given tillage method, with an increase in the number of irrigation events, spike number showed an increasing trend, thousand-grain weight showed a decreasing trend, and the regularity of kernel number per spike varied. However, while there was no significant difference between W0 and W1 in the first year of the experiment, the kernel number per spike under both the RT and SS conditions reached a maximum value in the W1 treatment in the two years. In 2016–2017, the average kernel number per spike in W1 increased by 21.08% and 8.63%, respectively, compared with that in W0 and W2.
According to the results of variance analysis, the effects of different combination treatments on thousand-grain weight and grain yield in the two-year test were obvious, while the effects on spike number and kernel number per spike were unstable. The tillage and irrigation obviously affected grain yields mainly by regulating the thousand-grain weight, and the spike number and thousand-grain weight, respectively. However, the interaction effects of tillage and irrigation were insignificant.
Effects of tillage and irrigation on root development indices and root architecture
The effect of tillage or irrigation on root parameters was significant and in different degree; however, the interaction effect of tillage and irrigation was not significant, and the main regulatory effect was of tillage (Tables 2 and 3). The total dry root weight (TDRW), total root length (TRL), total root surface area (TRSA) and total root volume (TRV) in the SS treatment in each growth period were all higher than those in the RT treatment. Compared with RT, TDRW, TRL, TRSA and TRV parameters in SS increased by 3.52–47.56%, 7.16–46.38%, 6.48–53.14% and 6.22–123.80% (2015–2016), and 6.54–34.38%, 5.16–54.87%, 12.54–49.09%, and 8.52–63.88% (2016–2017), respectively. Moreover, during the two-year test, the TDRW, TRL, TRSA and TRV in W1 were better than those in the other irrigation treatments. In comparison to W0, the above parameters in W1 increased by 1.06–53.78%, 3.33–29.42%, 4.78–60.44%, and 3.27–75.52% (2015–2016), respectively. And similar results appeared in 2016–2017 wheat growth season (Tables 2 and 3). Compared with W2, similar increasing proportions of the above parameters in W1 appeared with 1.71–12.02% and 1.20–8.22%, 1.12–11.02% and 1.33–15.15%, 2.01–11.33% and 0.92–10.57%, and 1.38–9.90% and 1.79–14.01% in two wheat growth seasons (2015–2016 and 2016–2017), respectively.
During the whole growth period, the root parameters under different combination treatments showed an inverted "V" trend, first increasing and then decreasing in both years. All root parameters in the SS treatment reached a peak at the anthesis stage, while those in the RT treatment mostly reached a peak earlier, at the heading stage. Moreover, four root configuration parameters (TDRW, TRL, TRSA and TRV) were the highest and lowest in SSW1 and RTW0 treatments, respectively.
The figures (Figs. 1; 2; 3; and 4) show that the distributions of root growth characteristic parameters in different soil layers were different, and in the same growth period, the DRWD, RLD, RSA, and RV of different combination treatments gradually decreased as the soil depth increased. Considering the whole growth season, the DRWD, RLD, RSA, and RV in different soil layers for each combination treatment exhibited a single peak curve, rising first and then falling.
As soil depth increasing, the root parameters varied. In the upper soil layers (0–40 cm), the DRWD, RLD, RSA and RV under the RT treatment all reached their maximum value at the heading stage, while the maximum values under SS were postponed to the anthesis stage. In the middle soil layers (40–60 cm), the maximum values of DRWD and RV under RT appeared at the heading stage and anthesis stage, respectively. The maximum values of RLD and RSA under RT were different in two years, which both appeared at the anthesis stage in 2015–2016 and the heading stage in 2016–2017. However, the peak appearance time of each root parameter under the SS treatment appeared at the anthesis stage. The change in root parameters below the 60 cm soil layers varied depending on the tillage method and growth year. In 2015–2016, the root growth parameters under RT peaked at the anthesis stage, except that of DRWD treated with RTW2, which appeared at heading. The DRWD under SS reached its maximum value at the anthesis stage, and the maximum values of RLD, RSA and RV mostly appeared at the filling stage. However, in 2016–2017, the root parameter peaks in each combination treatment appeared at the anthesis stage. As soil depth increased and the growth period advanced, the peak time of root configuration growth parameters treated with RT tended to be delayed, that is, from the heading stage to the anthesis stage. However, the peak times of RLD, RSA and RV under SS were further delayed to the filling stage.
The effects of tillage and irrigation on root parameters in the middle and upper soil layers were not obvious, but the regulation of roots in the soil below 60 cm was significant. In the deep soil below 60 cm, the post-peak attenuation values of all root parameters in W1 decreased by 22.57% and 7.85% in comparison with W0 and W2, respectively. Compared with RT, the attenuation values under SS were reduced by 23.79% on average. Moreover, the post-peak attenuation values of DRWD, RLD, RSA, and RV in SSW1 were significantly lower than those in other treatments, and especially, they were lower by 31.65%, 40.17%, 44.23% and 50.35% than RTW0, respectively, in which the attenuation decreasing rates were highest.
Effects of root cutting in different soil layer on yield
Root cutting in different soil layers (no root cutting, CK; at 40 cm below the surface, T-40; at 80 cm below the surface, T-80) had a significant regulatory effect on winter wheat yield, with the T-40 treatment having the strongest regulatory effect (Table 4). The contribution rates of roots in the 0–40 cm, 40–80 cm and below 80 cm soil layers to yield were 78.32%, 12.09% and 9.59%, respectively. Root cutting in different soil layers reduced yield, and compared with that in CK, the grain yield in T-40 and T-80 decreased by 21.68% and 9.59%, respectively. Between T-40 and T-80, the significant differences in kernel number per spike and grain weight were caused by 40–80 cm root system, and between CK and T-80, the significant difference in grain weight was mainly due to the root below 80 cm (Table 4). Thus, the root system in the 40–80 cm soil layer regulated the kernel number per spike and grain weight of wheat, while the roots below 80 cm mainly regulated grain weight to affect the yield.
The relationships among root parameters, grain yield, soil water content, soil water use efficiency and precipitation water use efficiency
According to the correlation analyses between root growth parameters and grain yield (see Supplementary Fig. S1 online), the growth parameters TDRW, TRL, TRSA and TRV were positively correlated with grain yield to different degrees during each growth period of winter wheat. For TDRW and TRL, the correlation coefficient in the middle and late growth periods was relatively large, while for TRSA and TRV, there was little difference during the growth period except at the heading stage. All the correlation coefficients reached a maximum at the heading stage. In addition, in terms of their correlations with grain yield, the root parameters at the early growth stage ranked as TRV > TRSA > TRL > TDRW, while at the late growth stage, they ranked as TDRW > TRL > TRSA > TRV. In addition, the above mentioned four root parameters were positively and significantly correlated with soil water content, soil water use efficiency and precipitation water use efficiency except for the relationship between TDRW and soil water content (see Supplementary Table S1 online).
Discussion
Effects of tillage and irrigation on the root spatiotemporal distribution
SS reduces soil compaction, increases DRWD and promotes root growth26. This study found that the promotion effects of tillage on root structure were embodied in the following aspects: the root parameter peak in the RT treatment appeared earlier, and all the growth characteristic parameters in the 1 m soil layer reached a maximum at the heading stage, in contrast, SS delayed root senescence, and the peaks of TDRW, TRL, TRSA, and TRV were postponed to the anthesis stage. This may be due to the increased soil water consumption, precipitation water use efficiency and irrigation water use efficiency in SS treatment6. Applying irrigation too early or too late during early growth is not conducive to root growth in the middle and late stages, and properly prolonging the irrigation date in the early growth stage can result in high yield and highly efficient root traits27. Studies have shown that irrigation at the jointing and anthesis stages could increase the absorption area of deep roots28. However, this study showed that the W1 irrigation mode slowed root senescence in the deep soil layer, because irrigation only at jointing can help roots penetrate the soil and increase the absorption and utilization of deep water29. Excessive irrigation causes much ineffective evapotranspiration at the late growth stage, and thus water use efficiency greatly decreases6. Furthermore, an appropriate irrigation deficit can enhance the soil enzyme activity in deep soil30.
The influence of tillage under different soil layers on root configuration remain unclear. We found that, with increasing soil depth, the maximum appearance time of each root parameter was delayed overall. The parameter peak in the deep soil layer under RT was postponed to the anthesis stage, while that under SS was postponed to the filling stage, and the post-peak attenuation decrease was smaller than that in the RT treatment. This may because deep ploughing can reduce the soil bulk density of the 20–40 cm soil layer in lime concretion black soil and increase the soil porosity and field water-holding capacity of the 20–40 cm layer31. And root growth parameters are determined by soil bulk density32. These suggest that tillage methods can promote root growth by improving soil structure. Previous studies usually focused on the effects of single factor (tillage or irrigation) on root growth of winter wheat33,34,35,36. However, there are few literatures about double factors and their interactions on root structures in different soil layers of winter wheat in lime concretion black soil. Ali and his colleagues found that, under the condition of 200 mm rainfall in a dry-land farming system, the ridge furrow rainfall harvesting technique with 150 mm deficit irrigation could significantly promote the physiological morphology of root system in the top 40 cm soil layer, resulting in higher grain yield37. In another experiment, no-tillage promoted the accumulated density in shallow root system under drought condition and this is highly dependent on surface irrigation water38. In this study, however, the regulation effects of tillage and irrigation on the root system were mainly manifested in the root system below 60 cm soil layer. The spatiotemporal distribution of root growth parameters in the 1 m soil layer under SSW1 was the best. Under this treatment, the peak appearance times of RLD, RSA and RV in deep soil layers were delayed. This study also indicated that under SSW1, the growth peak of the deep root system occurred later, and the post-peak attenuation decline was slowed. Thus, we consider a good root spatiotemporal distribution to be the main morphological or physiological factor causing high yield.
Effects of tillage and irrigation on grain yields
Xu et al.39 demonstrated that irrigation at anthesis and jointing with 150 mm of water was the best irrigation mode in the North China Plain. However, Meng et al.40 considered that irrigation applied at the wintering and jointing stages is good for nitrogen accumulation and utilization. If irrigation at anthesis was applied based on the pattern mentioned above, the proportion of accumulated nitrogen transferred to the grain would be significantly reduced, which would lead to decreases in nitrogen fertilizer utilization and yield. Similar results in single factor have previously been reported by Meng and his colleagues40, indicating that the yield in the treatment of irrigation at jointing stage was the highest. However, two tillage treatments on the basis of single irrigation factor have been added in our study. Compared with RT, SS increases the transpiration rate, net photosynthetic rate and water potential of flag leaves41. The results of our two-factor treatment showed that the yield in SSW1 treatment was highest in the lime concretion black soil of southeastern Henan. Zhang et al.42 reported that the TRSA, TRV and total number of root tips were significantly positively correlated with yield. In our study, root growth parameters were positively correlated with grain yields at different degrees, because there are positive correlations among root growth parameters, water use efficiency and nitrogen use efficiency43. In addition, root parameters in our study were significantly and positively correlated with soil water content, soil water use efficiency and precipitation water use efficiency. Thus, it has been speculated that the root growth parameters could significantly affect the water and nitrogen absorption of winter wheat, then regulate the kernels per spike and the thousand-grain weight, and ultimately affect grain yield. In particular, SSW1 can effectively delay root system senescence, maintain high root biomass and root length, and increase RSA and RV in the late growth stage, which play an important role in achieving high yield.
Root contributions at different soil layers to grain yields
Guo et al.44 showed that the roots in soil layers above 20 cm and below 1 m contributed mostly to yield, especially the wheat plant roots below 1 m, which were more important for yield when wheat suffered drought at the late stage of growth. Moreover, Wang et al.45 showed that the root system of summer maize in the 0–40 cm soil layer had the greatest influence on nitrogen accumulation and transport after anthesis and thus had the greatest impact on yield. However, the root system in the soil layer below 80 cm had a significant influence on the thousand-grain weight of summer maize. This study revealed that the root system at 0–40 cm soil depth made the largest contribution to yield, but the roots at the lower and middle depths below 40 cm contributed as much as 21.68% to yield. Yield mainly depends on the shallow root system with abundant root biomass, but the deep root system has significant effects on the yield of crops such as wheat and rice46,47. Therefore, the roots in deep layers play an important role in determining yield. For this reason, in production practices, promoting deep rooting and maintaining the quantity and activity of the deep root system should be key to achieving high and stable wheat yields.
Conclusions
In the lime concretion black soil, the root structures and distribution characteristics of winter wheat were positively related to soil water content, root water absorption characteristics and grain yields. SSW1 should be used as a high-yield and high-efficiency cultivation mode for winter wheat, and it was characterized with moderate root growth and distribution at the surface and upper soil layers but relatively large root amounts at the middle and lower soil layers. With this cultivation model, the growth peak of deep roots appeared later, and the post-peak attenuation decline was postponed. With this root distribution characteristic, water storage consumption at the middle and lower soil layers was effectively increased, much root biomass at late growth stages was sustained, water requirement during grain filling period was achieved, and water use efficiency was increased, resulting in the promoted dry matter productions and grain yields. In conclusion, the root proportions at deep soil layers can play partial roles in the potential of wheat grain yields. Improving the root distribution and absorption capacity at deep soil layers during cultivation helps obtain high grain yields and high water use efficiency.
Materials and methods
Study site
A field experiment was conducted in lime concretion black soil for two successive years from 2015 to 2017 at the 14th sub-farm of Shangshui County, Henan Province, China (33° 32′ N, 114° 29′ E). This experimental site is in a warm temperate continental monsoon climate, and the soil type is characterized with high viscosity, dry shrinkage and wet expansion, and shallow groundwater, as described in one previously published literature6. In our study site, there exists the lime concretion at 60–80 cm soil layers in our study site, and deep soil layers are relatively soft. The wilting coefficient and the available water content of the soil were 8.02% and 23.42%, respectively. Moreover, the annual average temperature in this area was 14.5 ℃, the accumulated sunshine duration was 2072.3 h, the average frost-free season was 223 d, and the annual average precipitation was 784.1 mm (meteorological data for 1997–2017, provided by the Shangshui Meteorological Bureau). The nutrient content of the plough layer soil was measured in 2015 before wheat sowing. The values observed in the 0–20 cm soil layer were as follows: organic matter, 21.32 g·kg−1; total nitrogen, 1.36 g·kg−1; Olsen phosphorus, 16.83 mg·kg−1; available potassium, 214.61 mg·kg−1; and pH, 7.23. Those in the 20–40 cm soil layer were as follows: organic matter, 16.06 g·kg−1; total nitrogen, 1.23 g·kg−1; Olsen phosphorus, 8.58 mg·kg−1; and available potassium, 163.26 mg·kg−1. The soil bulk density data before and after tillage are shown in Supplementary Table S2 online.
The precipitation amounts during the wheat growing periods in 2015–2016 and 2016–2017 were 281.3 mm and 360.9 mm, respectively. Precipitation and irrigation amounts at different growth stages in the two years are shown in Supplementary Table S3 online. The field water-holding capacity is shown in Supplementary Table S4 online. Solar radiation and daily average air temperature during the wheat growing stages were 12.80 MJ·m−2·d−1 and 9.52 ℃ (2015–2016), and 13.51 MJ·m−2·d−1 and 10.81 ℃ (2016–2017), respectively.
Experimental design
Field experiment
The crop previously grown in the experimental field was corn, and the straw was crushed and returned to the field. Wheat cultivar Zhoumai 27 used in this experiment is a commercial cultivar, which has been authorized by the China government in 2011 to be permitted to plant in China. In both years, wheat sowing was delayed by continuous rain before sowing. The tillage and sowing dates of the two years were October 27 and October 30 (2015–2016) and November 1 and November 4 (2016–2017), respectively. The sowing rate in both years was 217.50 kg·ha−1. The conditions at field emergence under different treatments and soil moisture content before sowing in the two-year test are shown in Supplementary Table S5 online. A Nonghaha 2BXF-12 wheat seeder was used for seeding, with a row spacing of 20 cm. A split block experiment was conducted. The main treatment was tillage, including SS and RT. The depths of SS and RT were 30–33 cm and 15–17 cm, respectively. The secondary treatment was irrigation, with three levels: W0, W1 and W2. Six treatments in different tillage and irrigation were placed randomly after distinguishing the main and secondary plots. Each irrigation amount was controlled at 75 mm, which was measured by a water meter. Impermeable planting areas with intervals of two metres were established between secondary treatments. Pure N (240 kg·ha−1), P2O5 (120 kg·ha−1), and K2O (90 kg·ha−1) were applied throughout the whole growth period of wheat. The phosphate and potash fertilizers were applied basally before sowing. Similar to W1 and W2 treatments, half of the N fertilizer amounts in W0 treatment were applied before sowing, and the remaining fertilizers were applied at jointing. Because precipitation occurred at jointing stage in the two wheat growth seasons, no irrigation was been performed in W0 treatment, and the remaining N fertilizers were applied during the jointing period according to the precipitation or the soil moisture. Each treatment was repeated three times, and the area of each plot was 60 m2 (10 m × 6 m). Pest, weed and disease control was carried out according to local guidelines.
Soil column experiment
In-situ soil column experiment was set in the field experiment. Before soil preparation, one repeated planting area of the field experiment was selected to plot a certain area. Soil in 1.5 m deep was turned over to the ground at six levels: 0–20 cm, 20–40 cm, 40–60 cm, 60–80 cm, 80–100 cm, and below 100 cm. Soil above 1 m was mixed thoroughly by layers, and soil bulk density of each soil layer was measured. The soil column was made according to the method of Fan et al.48, and appropriate improvements were made. Specific device was shown in Supplementary Fig. S2 online. In order to facilitate root extraction, root breaking and washing during wheat growth, PVP plastic tube with a height of 210 cm and an inner diameter of 40 cm was cut in half along the axial direction. Then cut into 150 cm, 80 cm, and 40 cm according to the root cutting height. The round tube was fixed with a round steel clip after cutting and marked at the pre-cut root. The gap was sealed with glass glue to make it impermeable. The tube bottom was fastened with a leaky iron plate, and a wire rope was fixed on it to facilitate lifting the soil column from the soil pit. Put the prepared soil column into the soil pit vertically. In order to reduce differences in soil layers, the field soils at each layer were separately sampled and transferred to the related layers of the PVP plastic tube used for the soil column experiment. Actual soil moisture contents were measured before loading soil layers. Soil of 0–20 cm should be mixed thoroughly according to the base fertilizer amount for each treatment in the field test and then backfilled. Root-breaking treatments were performed in the 0 cm (CK), 40 cm (T-40), and 80 cm (T-80) soil layers at anthesis. Three soil columns were made for each treatment, and a total of 54 soil columns were made by combining six treatments of field experiment. Soil around soil column was dug out at anthesis and all the roots were cut off at the mark with electric wire saws. In order to facilitate root cutting, soil columns with the same treatment were arranged together. Seedling amounts (30) in each soil column were same as the field experiment, and the sowing, topdressing and irrigation time were the same as the field. The wheat variety used was Zhoumai 27.
Contents of determinations and methods
Root parameters
Root samples were collected from the 0–100 cm soil layer in each plot at the jointing, heading, anthesis, filling and maturity stages, and every 20 cm of depth was regarded as one soil layer. The sampling method was the same as that used by Wang et al.7. After processing, the root parameters such as root length, root surface area and root volume in different soil layers were obtained by root system analysis software (WinRHIZO 2008). The root was dried with absorbent paper and then placed in an oven at a constant temperature of 80 ℃ to obtain the root dry weight. The dry root weight density (DRWD, g·m−3), root length density (RLD, cm·cm−3), root surface area per unit area (RSA, m2·ha−1) and root volume per unit area (RV, m3·ha−1) in different soil layers were determined by Eqs. (1), (2), (3) and (4), respectively49,50,51,52:
Total dry root weight (TDRW, g·m−2), total root length (TRL, cm·cm−2), total root surface area (TRSA, m2·ha−1) and total root volume (TRV, m3·ha−1) refer to the sums of root weight, root length, RSA, and RV per unit soil area in different soil layers53. They were determined by Eqs. (5), (6), (7) and (8), respectively:
where M, L, A, V', V and S are root dry weight (g), root length (cm), root surface area (m2), root volume (cm−3), soil volume (cm−3) and soil area (cm2), respectively. The soil layer number is represented by i. There are five layers in total, and n is the total number of soil layers. Moreover, Mi, Li, Ai and Vi' are the root dry weight, root length, root surface area and root volume of the i-th layer, respectively.
Grain yields and components
In each plot, the 6 m2 (2 m × 3 m)-wheat plants were manually harvested to measure grain yields. The fixed sampling points for one-metre double rows of each treatment were investigated at maturity to obtain the spike number. Fifty representative stems were selected for each repeated treatment in each plot during the harvest period, packed into mesh bags, marked and then taken back to the laboratory to measure the kernel number per spike. Grain weight was measured after threshing and drying and converted into actual yield according to a 13% water content.
Yield contribution rate
Wheat samples from the soil column experiment were harvested at maturity to measure wheat yield and three factors of yield. No root cutting (CK), cutting of wheat roots at 40 cm below the surface (T-40) and cutting of roots at 80 cm below the surface (T-80) are represented by Y0, Y40 and Y80, respectively. C40, C40-80 and C80 are the yield contribution rates of roots in the 0–40 cm, 40–80 cm, and below 80 cm soil layers, respectively. They were determined by Eqs. (9), (10) and (11), respectively:
Statistical analysis
Excel 2013 and Origin 2018 were used for data processing and graphing. Statistical analyses, significance tests (Duncan's test) and correlation analysis (Pearson) were performed using SPSS 23.0.
References
Uhlmann, N. K. & Beckles, D. M. Storage products and transcriptional analysis of the endosperm of cultivated wheat and two wild wheat species. J. Appl. Genet. 51, 431–447 (2010).
Sun, H. W. Moisture characters of calcic concretion black soil and their relationship with soil drought. Acta Pedol. Sin. 30, 423–431 (1993) ((in Chinese with English abstract)).
Chen, X. W. et al. Least limiting water range and soil pore-size distribution related to soil organic carbon dynamics following zero and conventional tillage of a black soil in Northeast China. J. Agric. Sci. 153, 270–281 (2015).
Huang, Y. & Shi, R. J. Study on the relationship between soil structure characteristics and large-scale mechanized tillage: a case study of cultivated land in black soil area. China Sci. Technol. Rev. 26, 301 (2015) ((in Chinese with English abstract)).
Aggarwal, P., Choudhary, K. K., Singh, A. K. & Chakraborty, D. Variation in soil strength and rooting characteristics of wheat in relation to soil management. Geoderma 136, 353–363 (2006).
Wang, Y. H. et al. Effects of tillage practices and irrigation times on water use efficiency and grain yield of winter wheat in lime concretion black soil. Scientia Agricultura Sinica. 52, 801–812 (2019) ((in Chinese with English abstract)).
Wang, Y. H. et al. Effects of cultivation patterns on winter wheat root growth parameters and grain yield. Field Crops Res. 156, 208–218 (2014).
Chu, P. F., Yu, Z. W., Wang, D. & Zhang, Y. L. Effects of tillage on water consumption characteristics and grain yield of wheat. Scientia Agricultura Sinica. 43, 3954–3964 (2010) ((in Chinese with English abstract)).
Zheng, C. Y. et al. Effects of soil tillage practice on dry matter production and water use efficiency in wheat. Acta Agronomica Sinica. 37, 1432–1440 (2011) ((in Chinese with English abstract)).
Wang, H. G., Yu, Z. W., Zhang, Y. L., Shi, Y. & Wang, D. Effects of tillage regimes on water consumption and dry matter accumulation in dryland wheat. Acta Agronomica Sinica. 38, 675–682 (2012) ((in Chinese with English abstract)).
Chen, S. Y., Sun, H. Y., Shao, L. W. & Zhang, X. Y. Performance of winter wheat under different irrigation regimes associated with weather conditions in the North China Plain. Aust. J. Crop. Sci. 8, 550–557 (2014).
Sun, H. Y., Zhang, X. Y., Wang, E. L., Chen, S. Y. & Shao, L. W. Quantifying the impact of irrigation on groundwater reserve and crop production—a case study in the North China Plain. Eur. J. Agron. 70, 48–56 (2015).
Zhou, L. L. et al. A simulation of winter wheat crop responses to irrigation management using CERES-Wheat model in the North China Plain. J. Integr. Agric. 17, 1181–1193 (2018).
Ali, S. et al. Deficit irrigation strategies to improve winter wheat productivity and regulating root growth under different planting patterns. Agric. Water Manag. 219, 1–11 (2019).
Zhang, X. Y., Chen, S. Y., Sun, H. Y., Wang, Y. M. & Shao, L. W. Root size, distribution and soil water depletion as affected by cultivars and environmental factors. Field Crops Res. 114, 75–83 (2009).
Liu, D. Y., Shi, L. Y., Huang, B. R. & Dong, Q. Y. Research of cultivation methods on root system root vigor and plant characteristics in winter wheat. Scientia Agricultura Sinica. 5, 51–56 (1993) ((in Chinese with English abstract)).
Yan, X. J., Zhang, S. Q., Wang, Z. R. & Sun, Y. Y. Responses of root growth and water use efficiency to planting density for winter wheat varieties from different eras in semi-arid region. Res. Soil Water Conserv. 20, 32–36 (2013) ((in Chinese with English abstract)).
Hu, C. L. et al. Root pruning enhances wheat yield, harvest index and water-use efficiency in semiarid area. Field Crops Res. 230, 62–71 (2019).
Sun, Y. Y. Research progress on effects of cultivation patterns on root development of wheat. Mod. Agric. Sci. Technol. 19, 4–6 (2017) ((in Chinese with English abstract)).
Wang, Y. Z., Liu, X. W., Sun, H. Y., Zhang, X. Y. & Zhang, L. R. Effects of water and nitrogen on root/shoot ratio and water use efficiency of winter wheat. Chin. J. Eco-Agric. 21, 282–289 (2013) ((in Chinese with English abstract)).
Juliane, H., Jens, L., Samuel, A. & Jochen, M. Maize and wheat root biomass, vertical distribution, and size class as affected by fertilization intensity in two long-term field trials. Field Crops Res. 216, 197–208 (2017).
Tian, B. J. et al. Limited-irrigation improves water use efficiency and soil reservoir capacity through regulating root and canopy growth of winter wheat. Field Crops Res. 196, 268–275 (2016).
Bhattacharyya, R., Kundu, S., Pandey, S. C., Singh, K. P. & Gupta, H. S. Tillage and irrigation effects on crop yields and soil properties under the rice–wheat system in the Indian Himalayas. Agr Water Manag. 95, 993–1002 (2008).
Kumar, G. D. et al. Mitigation of greenhouse gas emission from rice–wheat system of the Indo-Gangetic plains: Through tillage, irrigation and fertilizer management. Agric. Ecosyst. Environ. 230, 1–9 (2016).
Gajri, P. R., Prihar, S. S., Cheema, H. S. & Kapoor, A. Irrigation and tillage effects on root development, water use and yield of wheat on coarse textured soils. Irrigat. Sci. 12, 161–168 (1991).
Mu, X. et al. Responses of soil properties, root growth and crop yield to tillage and crop residue management in a wheat–maize cropping system on the North China Plain. Eur. J. Agron. 78, 32–43 (2016).
Zhang, X. Q., Cao, C. F., Qiao, Y. Q., Li, W. & Chen, H. Response of wheat root characteristics and canopy photosynthesis to different irrigation methods in lime concretion black soil. Scientia Agricultura Sinica. 48, 1506–1517 (2015) ((in Chinese with English abstract)).
Feng, S. W., Gu, S. B., Zhang, H. B. & Wang, D. Root vertical distribution is important to improve water use efficiency and grain yield of wheat. Field Crop Res. 214, 131–141 (2017).
Xue, L. H., Duan, J. J., Wang, Z. M., Guo, Z. W. & Lu, L. Q. Effects of different irrigation regimes on spatial-temporal distribution of roots, soil water use and yield in winter wheat. Acta Ecol. Sin. 30, 5296–5305 (2010) ((in Chinese with English abstract)).
Xue, L. H., Wang, Z. M., Guo, Z. W., Liu, Y. P. & Zhao, Z. G. Effects of different irrigation regimes on spatial-temporal distribution for activity of soil enzyme. J. Soil Water Conserv. 24, 228–232 (2010) ((in Chinese with English abstract)).
Zhang, Y. E. et al. Effects of tillage and nitrogen fertilization regimes on main physicochemical properties of soil and crop yield. Agric. Res. Arid Areas 36, 186–193 (2018) ((in Chinese with English abstract)).
Zhang, X. Y. & Hu, C. S. Root growth and distribution in relation to different water levels. Enhanc. Underst. Quantif. Soil-Root Growth Interact. 4, 45–65 (2013).
He, J. N., Shi, Y., Zhao, J. Y. & Yu, Z. W. Strip rotary tillage with a two-year subsoiling interval enhances root growth and yield in wheat. Sci. Rep. 9, 1–13 (2019).
Martinez, et al. Soil physical properties and wheat root growth as affected by no-tillage and conventional tillage systems in a Mediterranean environment of Chile. Soil Till. Res. 99, 232–244 (2008).
Lv, G. H., Kang, Y. H., Li, L. & Wan, S. Q. Effect of irrigation methods on root development and profile soil water uptake in winter wheat. Irrigat. Sci. 28, 387–398 (2010).
Wu, B. J., Zhang, H. B. & Wang, D. Timely supplemental irrigation changed nitrogen use of wheat by regulating root vertical distribution. J. Plant Nutr. Soil Sci. 181, 396–408 (2018).
Ali, S. et al. Tillage and deficit irrigation strategies to improve winter wheat production through regulating root development under simulated rainfall conditions. Agric. Water Manag. 209, 44–54 (2018).
Morio, et al. No-tillage enhanced the dependence on surface irrigation water in wheat and soybean. Plant Prod. Sci. 10, 182–188 (2015).
Xu, X. et al. Improving water use efficiency and grain yield of winter wheat by optimizing irrigations in the North China Plain. Field Crops Res. 221, 219–227 (2018).
Meng, W. W., Wang, D., Yu, Z. W. & Shi, Y. Effects of irrigation on nitrogen uptake and distribution of wheat using the 15N tracer technique. J. Plant Nutrit. Fertil. 17, 831–837 (2011) ((in Chinese with English abstract)).
Sang, X. G., Wang, D. & Lin, X. Effects of tillage practices on water consumption characteristics and grain yield of winter wheat under different soil moisture conditions. Soil Till Res. 163, 185–194 (2016).
Zhang, X. Q., Cao, C. F. & Qiao, Y. Q. Effects of different irrigation methods on the root, photosynthesis and quality of wheat. Acta Agriculturae Boreali-Sinica. 31, 212–217 (2016) ((in Chinese with English abstract)).
Wang, Y. H. Study on relations among construction of wheat root system, yield, and utilization of water and nitrogen under different cultivation patterns. Henan Agricultural University. (2014).
Guo, C. H., Miao, G. Y. & Gao, Z. Q. Effect of pruning root in grain filling stage on yield and physiological characteristic of winter wheat. J. Triticeae Crops. 27, 331–334 (2007) ((in Chinese with English abstract)).
Wang, F. F. et al. Effect of root in different soil layers on plant growth and yield formation after anthesis in summer maize. Scientia Agricultura Sinica. 46, 4007–4017 (2013) ((in Chinese with English abstract)).
Zhu, F. D. & Lin, X. Q. Effects of deep roots on growth and yield in two rice varieties. Scientia Agricultura Sinica. 34, 429–432 (2001) ((in Chinese with English abstract)).
Liu, B. H., Liu, Z. H., Zhang, W. Y., Li, Y. & Li, Y. Q. Effects of roots-cutting on the photosynthetic rate and growth of winter wheat of different root types. Acta Agriculturae Boreali-Sinica. 23, 99–103 (2008) ((in Chinese with English abstract)).
Fan, J., Qi, L. B., Shao, M. A. & Wang, Q. J. Method for measuring soil water absorption process of plant roots (in Chinese with English abstract) (2008).
Mosaddeghi, M. R., Mahboubi, A. A. & Safadoust, A. Short-term effects of tillage and manure on some soil physical properties and maize root growth in a sandy loam soil in western Iran. Soil Till Res. 104, 173–179 (2009).
Li, Q. Q., Dong, B. D., Qiao, Y. Z., Liu, M. Y. & Zhang, J. W. Root growth, available soil water, and water-use efficiency of winter wheat under different irrigation regimes applied at different growth stages in North China. Agr Water Manag. 97, 1676–1682 (2011).
Wang, C. Y. et al. Effects of different irrigation and nitrogen regimes on root growth and its correlation with above-ground plant parts in high-yielding wheat under field conditions. Field Crops Res. 165, 138–149 (2014).
Guan, D. H. et al. Tillage practices effect on root distribution and water use efficiency of winter wheat under rain-fed condition in the North China Plain. Soil Till Res. 146, 286–295 (2015).
Wang, Y. H. et al. Effects of different cultivation patterns on the spatial-temporal distribution characteristics of roots and grain yield of winter wheat in two climatic years. Scientia Agricultura Sinica. 45, 2826–2837 (2012) ((in Chinese with English abstract)).
Acknowledgements
This work was supported by the National Natural Science Foundation of China (Project No. 31471439) and the National Agriculture Technology Research System of China (Project No. CARS-03).
Author information
Authors and Affiliations
Contributions
Y.W. and T.G. designed the research; J.W. wrote the main manuscript text; J.W. and Z.W. analyzed the data; Z.W. drew the figures; F.G. and H.L. collected the data; G.K. and W.F. contributed to the revised manuscript. All authors discussed the results and commented on the manuscript at all stages.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Wang, J., Wang, Z., Gu, F. et al. Tillage and irrigation increase wheat root systems at deep soil layer and grain yields in lime concretion black soil. Sci Rep 11, 6394 (2021). https://doi.org/10.1038/s41598-021-85588-6
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-021-85588-6
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.