As the global population continues to increase, global food production needs to double by 2050 to meet the demand. Given the current status of the not expansion of cultivated land area, agronomic seedlings are complete, well-formed and strong, which is the basis of high crop yields. The aim of this experiment was to study the effects of seed germination and seedling growth in response to silicon (from water-soluble Si fertilizer). The effects of Si on the maize germination, seedling growth, chlorophyll contents, osmoprotectant contents, antioxidant enzyme activities, non-enzymatic antioxidant contents and stomatal characteristics were studied by soaking Xianyu 335 in solutions of different concentrations of Si (0, 5, 10, 15, 20, and 25 g·L−1). In this study, Si treatments significantly increased the seed germination and per-plant dry weight of seedlings (P < 0.05), and the optimal concentration was 15 g·L−1. As a result of the Si treatment of the seeds, the chlorophyll content, osmotic material accumulation and antioxidant defence system activity increased, reducing membrane system damage, reactive oxygen species contents, and stomatal aperture. The results suggested that 15 g·L−1 Si significantly stimulated seed germination and promoted the growth of maize seedlings, laying a solid foundation for subsequent maize growth.
Maize, an annual herbaceous plant species, is one of the most important crop species and is a major source of feed and industrial raw materials1. Maize plays an important role in ensuring food security and social and economic stability. In recent years, due to the increasing frequency of extreme weather events, unstable yields and low quality of maize caused by seed germination problems in the cold regions of northern China have become an important hidden danger of China's food security2. Seeds are the result of survival mechanisms of plants and constitute the material basis for production. The growth status during seed germination directly affects the growth of plants3. The rapid germination of maize seeds after sowing leads to early-stage seedlings, homogeneous seedlings and robust seedlings, which constitutes the solid foundation for producing high-quality and high-yield of crops4.
As a basic element of animals and humans, Si is a beneficial but unnecessary element of higher plants4,5. Although the importance of Si to plants has not been shown, its beneficial role in promoting plant growth has been demonstrated in laboratory and field experiments6. Si can enhance seed vigor, increase seedling relative growth rates, increase seedling dry weight, and promote seedling cotyledon growth, laying a foundation for increasing yield and improving quality7,8,9,10.
Si is listed as the fourth element after nitrogen (N), phosphorus (P) and potassium (K) in the international soil classification system. Although soils are rich in Si, most Si cannot be fully absorbed by plants11. At present, there are two main types of Si fertilizer in China: citrate-soluble and water-soluble Si fertilizer. Citrate-soluble Si fertilizer is insoluble in water but soluble in acid; it can be absorbed by crops immediately after application. Citrate-soluble Si typically is a waste product of steel slag during steelmaking, fly ash, or ore through high-temperature calcination process, etc. However, because the raw material is mainly industrial waste and the manufacturing process is relatively simple; many toxic substances from industrial production remain in this type of fertilizer. Water-soluble Si fertilizer is soluble in water and can be directly absorbed and utilized by plants. It is synthesized by high-temperature chemical reactions from a complex production technique that is expensive and produces a relatively small amount12.
Si-50G and Si-60G are new water-soluble Si fertilizers synthesized by sol method. The Si-50G and Si-60G have been reported to increase the yield of rice, the state of soil Si and the soil available nutrient content under low temperature stress13,14. Although several studies have shown that Si treatment can promote rice growth, its effects on maize seed germination and growth are poorly understood. The objective of this work was to evaluate the effects of different concentrations of Si (from water-soluble Si fertilizer) on seed germination and seedling growth. The reaction was evaluated on the basis of the germination index, growth index, chlorophyll content, peroxidase content, antioxidant enzyme activity, non-enzymatic antioxidant content and stomatal characteristics. With this information, we can better understand the possible mechanisms by which Si promotes maize seed germination and seedling growth to select the best type and concentration, providing a scientific basis for Si application in maize production.
Effects of Si on the germination indices
As shown in Table 1, the Si-50G treatments (5 ~ 25 g·L−1) and the Si-60G treatments (5 ~ 20 g·L−1) could promote the germination of maize seeds, resulting in GR, GP, GI and VI values that were higher than those in the control group. However, Si-60G application decreased the GR, GI and VI in the 25 g·L−1 treatments. Compared to those of the control, the GR, GP, GI and VI under a concentration of 15 g·L−1 (Si-50G) markedly increased by 19.32%, 38.89%, 53.70% and 129.01%, respectively (P < 0.01). With the increase of concentration, the content of Si first increased but then decreased, and the effects were the most obvious at the concentration of 15 g·L−1.
Effects of Si on seedling growth
With the increase of concentration, the growth indices of the seedlings first increased but then decreased, and the effects were the most obvious at the concentration of 15 g·L−1 (Table 2). Compared with those of the control, the shoot height, root height, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight under Si-50G 15 g·L−1 increased by 22.66%, 22.90%, 51.93%, 62.40%, 45.83%, and 50.00%, respectively; furthermore, compared with those of the control, increased by 9.10%, 13.24%, 45.01%, 51.55%, 18.75% and 46.88% under the Si-60G 15 g·L−1, respectively. Moreover, compared with those of the control, the shoot height, root height, shoot fresh weight, root fresh weight, shoot dry weight under the Si-60G 25 g·L−1 decreased by 9.04%, 2.46%, 2.24%, 3.10% and 6.25%, respectively. Compared with that of the control, the Si content under 15 g·L−1 increased by 4.69% and 4.38% (P < 0.01).
Effects of Si on the photosynthetic pigment contents
The Si-50G treatments (5 ~ 25 g·L−1) markedly increased the Chl a + b content, the Chl a content, the Chl b content, and the Car content by 0.92% ~ 6.81%, 1.49% ~ 5.58%, 0.29% ~ 8.27%, and 3.09% ~ 6.99%, respectively (Table 3). The Si-60G treatments (5 ~ 20 g·L−1) markedly increased the Chl a + b content, the Chl a content, the Chl b content, and the Car content by 0.27% ~ 3.30%, 0.60% ~ 4.48%, 0.29% ~ 1.98%, and 2.87% ~ 5.88%, respectively. Compared with those of control, Chl a + b, Chl a , Chl b and Car contents under the Si-60G 25 g·L−1 decreased by 1.91%, 1.42%, 2.88% and 2.21%, respectively.
Effects of Si on osmolyte contents
As shown in Table 4, except for the sugar and proline contents under the Si-60G 25 g·L−1, the soluble sugar, soluble protein and proline contents in maize leaves significantly increased with Si application compared with the control (P < 0.05). Compared with those of the control, the soluble sugar, soluble protein and proline contents under the Si-50G at 15 g·L−1 significantly increased by 23.89%, 34.07% and 27.27%, respectively (P < 0.01); furthermore, compared with those of the control, the same parameters under the Si-60G at 15 g·L−1 increased by 20.69%, 31.42% and 20.13%, respectively (P < 0.01). However, the soluble sugar and proline contents decreased by 2.46% and 1.32%, respectively, and the soluble protein contents significantly decreased by 5.75% compared with the control values.
Effects of Si on reactive oxygen species
Si reduced the generation rate of O2·− and H2O2 content in seedlings, and the effects were more pronounced under 15 g·L−1 (Fig. 1a,b). Compared with the control values, the Si-50G treatments decreased the generation rate of O2·− by 2.90%, 5.20%, 8.50%, 6.28% and 5.67% and decreased the H2O2 content by 5.90%, 7.41%, 9.70%, 8.20% and 4.63%, respectively. Moreover, the Si-60G at 5 ~ 20 g·L−1 treatments decreased the generation rate of O2·− by 2.19%, 4.29%, 6.38% and 3.04%, respectively, and decreased the H2O2 content by 4.23%, 5.61%, 8.58% and 5.98%, respectively. However, the Si-60G at 25 g·L−1 treatment increased the generation rate of O2·− and the H2O2 content by 0.94% and 2.70%, respectively.
Effects of Si on lipid peroxidation
The non-Si-treated maize seedlings had a higher membrane permeability and MDA content. However, these parameters nearly significantly decreased compared with those of the control when Si was present. Compared with those of the control, the MDA content and EL under the Si-50G 15 g·L−1 significantly decreased by 46.17% and 20.93% (Fig. 1c) and by 9.17% and 7.36% under the Si-60G 15 g·L−1 (Fig. 1d), respectively (P < 0.01).
Effects of Si on antioxidant enzyme activities
Compared with those of the control, the activities of SOD, POD, CAT and APX in the leaves in response to Si application increased, and the GR activity decreased (Fig. 2). Compared with that of the controls, the SOD, POD, CAT and APX increased by 16.16%, 12.08%, 18.73% and 15.38%, respectively, and by 31.31% and 27.89%, 21.31% and 20.06%, respectively at the 15 g·L−1(P < 0.01). However, compared with that of the control, the GR activity was decreased by 32.21% and 18.12%, respectively at the 15 g·L−1 (P < 0.01) (Fig. 2e).
Effects of Si on non-enzymatic antioxidant contents
Compared with those of the control, the GSH and AsA contents under Si-50G at 15 g·L−1 clearly increased by 28.05% and 18.29%, respectively (Fig. 3a); furthermore, compared with those of the control, the same parameters in the Si-60G at 15 g·L−1 treatment group increased by 16.46% and 13.50%, respectively (P < 0.01) (Fig. 3b).
Effects of Si on stomatal size and aperture
The size of stomatal notably changed. With the increasing Si concentration, the length and width of the stomata and the stomatal aperture first increased but then decreased. However, with the increasing concentration, the width of stomatal aperture first decreased but then increased. In addition, under the 15 g·L−1, the width of stomatal aperture significantly decreased by 24.34% and 17.11% (P < 0.01), respectively, compared with those of the control (Table 5).
The GR, GP, GI and VI of seeds are important indicators to measure the germination performance of plant seeds15. Seed germination and seedling emergence are the most critical and sensitive stages in plant life cycle. The results of this study show that the Si-50G treatments (5 ~ 25 g·L−1) and the Si-60G treatments (5 ~ 20 g·L−1) could significantly improve the GR, GP, GI and VI of maize seeds (Table 1). This indicates that the appropriate concentration of Si can help maize seeds break dormancy, accelerate the restoration of seed vigor and recovery from damage when drying seeds, complete the germination and emergence process, and provide better physiological conditions for subsequently seedling growth2.
Exogenous Si can promote the growth of soybean16, barley17 and seedlings of other species. Moreover, exogenous Si can increase the root length and seedling height and can increase the fresh and dry weight of seedlings. In this study, most of the Si treatments enhanced the height, length and dry weight of maize seedlings (Table 2). The highest germination percentage was obtained under the Si-50G at 15 g·L−1, and the total dry and fresh weight of seedlings significantly increased. The effects of 15 g·L−1 Si on the maize root system was better than that on the whole plants. The establishment of seedlings after seed germination is the period that is most sensitive to the external environment. A healthy and intact root system can ensure that plants can absorb sufficient amounts of water, nutrients and mineral ions from the soil and promote photosynthesis of plant leaves to complete self-growth2.
Chl a and Chl b are the main pigments that absorb and transmit light energy, which can improve the efficiency of light capture18. Car is photosynthetic pigment that absorbs light energy and transmits it to the reaction centers. Our results suggested that by 15 g·L−1 Si could increase the contents of Chl a + b, Chl a, Chl b and Car (Table 3). The application of Si may be associated with an increase in chlorophyll content, which may be because Si can eventually enter into the chloroplast through plant absorption and transport, increasing the chlorophyll content of the plant and thus increasing photosynthesis19,20. However, the Si-60G at 25 g·L−1 reduced the contents of Chl a + b, Chl a, Chl b and Car in maize seedlings. This may be due to the excessive concentration of Si, which reduces chlorophyll synthesis, while a decrease of soluble sugar content both inhibits stomatal opening and normal photosynthesis21.
As osmotic regulators, soluble sugar and proline can effectively reduce the osmotic potential of plant cells by increasing its content22,23. The osmotic function of soluble protein is well known24. Si increased the content of soluble sugar, soluble protein and proline in maize seedlings (Table 4) and decreased the cell fluid osmotic potential (Fig. 2d), indicating that Si could play an important regulatory role in maintaining normal cell metabolism. In addition, according to the above experiments, Si can promote the synthesis of soluble sugar, soluble protein and proline, maintain its contents at a high level, and increase the ability of osmotic regulatory ability of seedlings. With applications of Si, the increase in these permeable substances may be an effective mechanism to improve the ability of plants to resist oxidative stress25.
Under adverse conditions, plants produce an excess amount of reactive oxygen species (ROS)26, which can cause membrane peroxidation and electrolyte leakage in cells, eventually destroying the structure and function of the cell membrane27,28,29. However, the protective system of the organisms can remove these ROS and reduce harm. SOD, POD, CAT, APX and GR are the main enzymes involved in the protection system, and these enzymes play a key role in enhancing plant stress resistance and maintaining normal plant growth, metabolism and development30. Changes in the activities of these enzymes reflect the protective response of plants. SOD is a purifier of O2·− and protects cells by removing O2·− and reducing the concentration of other reactive oxygen species produced by O2·−. POD is a protective enzyme with high activity that is widely present in plants; it plays an important role in cell metabolism. The increase in POD activity is beneficial to plant growth and development31. CAT can remove H2O2 produced by stress in plants and decompose the accumulated H2O2 into H2O and O2, thus reducing its ability to damage plant tissues oxidatively32. The enzymatic antioxidants APX and GR, as well as non-enzymatic antioxidants AsA and GSH, are important components to eliminate ROS and reduce oxidative damage under abiotic stress33. These antioxidants have their own functions and maintain the redox state of cells34,35. In this study, the appropriate concentration of Si could enhance the activity of SOD, POD, CAT and APX and the content of AsA and GSH; reduce the content of MDA; effectively enhance the ROS-scavenging ability of plants to maintain the balance of ROS metabolism; reduce oxidative stress reactions; and reduce the degree of cell EL, thus promoting the growth of maize10,36. However, the Si-60G at 25 g·L−1 treatment resulted in an increase in the content of MDA and EL, which may be related to the accumulation of O2·− and H2O2, leading to damage to the biofilm system, and thus inhibiting the growth of seedlings (Table 2)29. Si can affect the accumulation of ROS in plants, help maintain the balance of reactive oxygen metabolism, reduce membrane peroxidation, maintain the integrity of the membrane system, and enhance the activity of enzymes involved in the antioxidant system, thus promoting plant growth.
Stomatal size affects plant water-use efficiency, photosynthetic rate and yield37. The stomata control the exchange of water vapor and carbon dioxide between the blade's interior and the atmosphere. The adjustment of the stomatal aperture can affect the progress of photosynthesis in plants38. In this study, Si reduced the stomatal width and facilitates stomatal opening and closing (Table 5). These findings are consistent with those from Laza's research on rice39. Many small spheres with a metallic luster were found around the stomata on the adaxial side of leaves under the Si-60G treatment at a concentration of 15 g·L−1 (Fig. 4b–d), compared with the stomata on the abaxial side (Fig. 4a). The element distribution on the surface of the sphere was subsequently analysed by energy dispersive spectrometry (EDS) (Fig. 5c). The distribution of the main elements on the surface of the spheres was shown (Fig. 5a), and many P elements were found on the surface of the spheres (Fig. 5b), which corroborates the findings of other researchers40,41. The P uptake of maize in response to Si fertilizer significantly increased, and there was a significant positive correlation between P uptake and Si uptake of maize. In the future, we need to further determine the route of P transport in maize and study the mechanism by which Si alleviates drought stress.
The present results showed that exogenous Si can improve the seed germination rate, germination potential, germination index and vigor index; promote seedling growth; and increased chlorophyll contents. In addition, Si could improve the antioxidant defence ability of maize plants and increase the contents of osmotic substances, thereby increasing the ability to remove ROS and maintain the integrity of the membrane system. Among the Si treatments, Si exerted the best positive effects at 15 g·L−1, while negative effects occurred in response to 25 g·L−1 (Si-60G) these findings indicate that the appropriate concentration of exogenous Si can promote maize seed germination and seedling growth laying a good foundation for subsequent growth.
Materials and methods
Plant material and treatments
This experiment was conducted in a controlled growth chamber at the Northeast Agricultural University of Harbin, Heilongjiang Province, China (45° 448′ 52″ N, 126° 43′ 9″ E). Exogenous Si compounds were provided by the school of Chemistry and Chemical Engineering, Harbin Institute of Technology. The characteristic features of Si preparation given in Table 6 and the particle size of Si preparation is shown in Fig. 6. The tested maize cultivar was Xianyu 335. Healthy, plump and pest-free maize seeds were sterilized for 20 min with 30% (w/v) hydrogen peroxide (H2O2), and then repeatedly rinsed and soaked abundantly with distilled water. Afterward, we wiped the water off the maize seeds surface with a filter and set the seeds aside. Five replicates (thirty seeds each) were placed on two layers of filter paper in 9 cm diameter petri dishes containing 15 mL of distilled water (control), and different concentration of exogenous Si (5, 10, 15, 20, and 25 g·L−1). The seeds were then allowed to germinate under the following environmental conditions: average day/night temperature of 25/18 °C, relative humidity of 65 ± 5%, light intensity of 350 μmol·m−2·s−1 and photoperiod of 16 h. To ensure the relative stability of each treatment concentration, the solution was supplemented once every 24 h and the number of maize seeds that germinated was recorded once every 24 h.
After the germination (for 7 days), relatively uniform seedlings were selected and transferred to opaque plastic pots (inner diameter, 15.5 cm; external diameter, 18 cm; and height, 11 cm) filled with 1.25 kg of soil per pot under the same environmental conditions as those reported above. The soil used in the experiment was black soil. The physical and chemical properties of the soil were as follows: pH, 8.06; organic matter, 46.52 g·kg−1; total nitrogen, 3.58 g·kg−1; total phosphorus, 1.21 g·kg−1; total potassium, 182.7 mg·kg−1; available phosphorus, 51.39 mg·kg−1; available nitrogen, 144.90 mg·kg−1; and Si, 78.8 mg·kg−1. Maize seedlings at the three-leaf stage were used for the treatments with concentrations (5, 10, 15, 20 and 25 g·L−1) of Si and distilled water (control). The seedlings were sprayed once every 3 days, for a total of 2 times. Five millilitres was sprayed onto both sides of each leaf. Each treatment was repeated 4 times for a total of 44 pots.
Whole plants were sampled from each treatment on the 7th day for measurements of the growth parameters. The second fully developed leaves of the seedlings were removed on the 7th day for measurements of the growth parameters. The second fully developed leaves of the seedlings were removed on the 7th day for scanning electron microscope observations and measuring the electrolyte leakage (EL). The remaining leaves were immediately frozen in liquid nitrogen and stored at − 80 °C for subsequent determination of the other indicators42.
Measurements of germination
Seeds were considered to have germinated when the radicle extended for at least 2 mm. After seven days of germination, the germination rate (GR), germination potential (GP), germination index (GI) and vigour index (VI) were measured according to following Eqs.10:
where, n is the number of germinated seeds and N represents the total number of seeds tested;
where, a is the number of germinated seeds after three days;
where, Gt is the number of seeds germinated on t days and Dt represents the corresponding day of germination; and
Measurements of plant growth
The maize seedlings with their roots were collected and rinsed with tap water and distilled water. The maize seedlings were then separated into roots and shoots, which were weighted separately to determine the fresh weight (FW) of each part. The height of the shoots and the length of roots of each plant were simultaneously measured by ruler. Dry weight (DW) was measured after the samples had dried in an oven dried at 105 °C for 30 min and had maintained constant weight at 80 °C for 24 h.
Measurements of pigment contents
Total chlorophyll, chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoid (Car) compounds were extracted, after which their contents were measured and quantified according to modified method of Arnon43. After the extraction and analysis, the relative contents of Chl a, Chl b, Car and the total content of chlorophyll were calculated according the following formulae:
where, V represents final volume of chlorophyll extract in 80% acetone and W is 0.1 g.
Measurement of osmoprotectants
The soluble sugar was quantified by the anthrone sulfuric acid method using 1% anthrone in concentrated H2SO4 as reagent44. Its absorbance was measured at 630 nm, and a standard curve was plotted with 0 ~ 100 mg of glucose.
The soluble protein was measured according to the coomassie brilliant blue G-250 method described by Bradford45. Coomassie brilliant blue G-250 (100 mg) was dissolved in 50 mL 95% ethanol. Afterward, 100 mL 85% (w/v) phosphoric acid was added to the solution. The resulting solution was diluted to a final volume of 1 L. The reagent ultimately consisted of 0.01% (w/v) coomassie brilliant blue G-250, 4.7% (w/v) ethanol, and 8.5% (w/v) phosphoric acid, and its absorbance was measured colorimetrically at 595 nm.
Briefly, 100 mg of tissue powder was homogenized in 10 mL of 3% aqueous sulfosalicylic acid for 10 min followed by filtration. Two milliliters of the filtrate, 2 mL of glacial acetic acid and 2 mL of acid ninhydrin were mixed together for 1 h at 90 °C. The sample was extracted in 4 mL toluene and measured colourimetrically at 520 nm against toluene, and a standard curve with proline was used for the final calculations46.
Measurements of reactive oxygen species
First, 0.5 mL of the reaction mixture, 0.5 mL sulfanilic acid solution and 0.5 mL α-naphthylamine solution were mixed together and shaken evenly. After incubating at room temperature for 20 min, the optical density of the mixture was determined at 530 nm via a spectrophotometer47.
H2O2 was extracted by homogenizing 0.3 g leaf tissue with 3 mL of 50 mM phosphate buffer (pH 6.5) together. The homogenate was centrifuged at 6000 rpm for 25 min. The supernatant was brought up to 3 mL with the phosphate buffer. To determine H2O2 content, 3 mL of extracted solution was mixed with 1 mL of 0.1% titanium sulphate in 20% H2SO4 (v/v) were mixed together, after which the mixture was then centrifuged at 6,000 rpm for 15 min. The intensity of the yellow color of the supernatant was measured at 410 nm. The H2O2 content was calculated from a standard curve prepared via hydrogen peroxide of known strength48.
Measurements of lipid peroxidation
Plant samples (0.4 g) were homogenized with 2.4 mL 0.1% trichloroacetic acid (TCA) and the homogenates were centrifuged at 12,000 rpm for 15 min. The supernatant (0.5 mL) and 20% TCA 1:1(v/v) were containing 0.5% (w/v) thiobarbituric acid (TBA) were mixed together (1:1), after which the solution was heated at 100 °C for 20 min. The absorbance was subsequently determined at 450, 532, and 600 nm25,49.
EL was used to evaluate plasma membrane permeability and measured using an electrical conductivity meter (DDS-307A, Inasa Analytical Instrument Co., Ltd., Shanghai, P. R. China). Each 0.5 g of fresh leaf material of maize seedling was placed in individual stoppered triangular flasks containing 10 mL of deionized water. The samples were incubated at 25 °C for 24 h. The electrical conductivity of the solution (S1) was measured after incubation. The samples were then placed in a boiling water bath for 10 min and the second measurement (S2) was determined after the solutions cooled to room temperature. EL = S1/S2 × 100%50.
Measurements of antioxidants
Crude enzyme extracts were prepared according to the method described by Pan et al.51. A total of 0.5 g of frozen leaf samples from maize seedlings were ground to a fine powder with liquid nitrogen using a chilled mortar and pestle and then homogenized in 5 mL extraction buffer containing of 50 mM phosphate buffer (pH 7.8), 0.1 mM ethylenediaminetetraacetic acid (EDTA), 0.3% (v/v) Triton X-100, and 4% gpolyvinylpyrrolidone (PVP). The homogenate was then immediately centrifuged for 20 min at 12,000 rpm. Phosphate buffer was subsequently added to the supernatant, which was used to analyse the activity of antioxidant enzymes. All operations were carried out at 0–4 °C.
Superoxide (SOD) activity was measured according to its ability to inhibit the photochemical reduction of nitro blue tetrazolium (NBT), which was recorded at 560 nm. One unit of SOD was defined as the amount of enzyme that inhibited NBT reduction by 50%52.
Catalase (CAT) activity was measured according to its ability to consume the amount of H2O253. The CAT reaction solution comprised 50 mM phosphate buffer (pH 7.8), 0.1 M H2O2 and 0.1 mL enzyme extract. Changes in the absorbance of the reaction solution at 240 nm were recorded for 5 min. One unit of CAT activity was defined as the absorbance change of 0.01 units per minute.
Peroxidase (POD) activity was determined using guaiacol as the substrate and assayed according to the method of Zheng et al.54 with minor modifications. First, 0.2 mL of the enzyme extract, 2 mL of 10 mM Na-phosphate buffer (pH 7.4), 0.1 mL of 1% guaiacol(v/v) , and 0.1 mL 0.3% H2O2 (v/v) were mixed together, after which the homogenates were centrifuged at 14,000 rpm for 20 min. The change in absorbance was measured at 470 nm for 5 min via a UV–visible spectrophotometer (T6, Persee, Beijing, P. R. China). One unit of POD activity was defined as an absorbance change of 0.01 units per minute.
In total, 0.5 g of seedling tissue was homogenized at 4 °C in 1 mL of extraction buffer [50 mM potassium phosphate buffer (pH 7.0), 1% Triton X-100, and 7 mM 2-mercaptoethanol] with mortar and pestle. The homogenate was then centrifuged at 25,000 rpm for 20 min, after which the supernatant was used as the crude extract for the ascorbate peroxidase (APX) and glutathione reductase (GR) assay55.
A 3 mL reaction mixture was composed of 50 mM phosphate buffer (pH 7.0) that consisted of 0.1 mM EDTA, 0.3 mM ascorbic acid (AsA), 0.06 mM H2O2 and 0.2 mL of enzyme extract. The homogenate was then centrifuged at 10,000 rpm for 20 min .The oxidation of ascorbate was followed by the decrease in the absorbance at 240 nm55.
GR activity was assayed by measuring the decrease in absorbance at 340 nm due to the oxidation of reduced nicotinamide adenine dinucleotide phosphate (NADPH)55. A 3 mL reaction mixture comprised 50 mM phosphate buffer (pH 8.2) that consisted of 1 mM EDTA, 0.2 mM NADPH, 1 mM oxidized glutathione (GSSG) and 0.2 mL of enzyme extract.AsA content was determined according to the method of Li et al.56 with minor modifications. Standards for AsA were prepared in the range of 0 ~ 60 μg in 1 mL of 5% (w/v) TCA, 1 mL alcohol, 0.5 mL 0.4% H3PO4-alcohol, 1 mL of 0.5% bathophenanthroline (BP)-alcohol and 0.5 mL of 0.03% FeCl3-alcohol at 30 °C for 60 min and measured at 534 nm. One gram of leaf tissue was ground in a chilled mortar and pestle in 5 mL of 5% (w/v) TCA. The homogenates were centrifuged at 4000 rpm for 10 min at 4 °C. The supernatant (1 mL) and 1 mL of 5% (w/v) TCA were mixed together according to the method of generating a standard curve. For each sample the AsA content was estimated as described above. To determine the reduced glutathione (GSH) content, 0.1 g frozen leaf tissue was ground in a chilled mortar and pestle with 3 mL of 5% (v/v) TCA. The homogenates were subsequently centrifuged at 2500 rpm for 15 min at 4 °C. The supernatants were then analyzed for their content of GSH57. Changes in absorbance of the reaction mixture were measured at 420 nm.
Measurement of Si content
Si content of maize seedlings was determined colorimetrically by the molybdate blue method and the absorbance was observed at 630 nm. Different amounts of silicic acid were included as standards for determining Si content of plant samples25.
Scanning electron microscopy-based measurements of the stomata
The leaf samples were cut into pieces of approximately 2 × 5 mm in size and fixed for more than 1.5 h with 2.5% glutaraldehyde in 0.1 M phosphate-buffered saline (PBS; pH 6.8) solution. The fixed samples were washed three times with the same solution for 10 min each. Afterward, the samples were dehydrated one time in a graded ethanol series (50%, 70%, and 90%) and two times in absolute ethanol for 10 min. The samples were displaced miscible liquids (comprising a 1:1 mixture of tertiary butanol and absolute ethyl alcohol) and absolute ethyl tertiary butanol for 15 min. The samples were maintained at − 20 °C for 30 min and dried in a freeze-drier (ES-2030, Hitachi, Tokyo, Japan) for 4 h. The observed samples faced up and were adhered to the scanning electron microscope sample table with conductive tape. The sample surfaces were coated with 100–150 Å metal films by ion sputter (E-1010, Hitachi, Tokyo, Japan). The sections were mounted on the sample table, observed with a scanning electron microscope and imaged(S-3400N, Hitachi, Tokyo, Japan).
The size and aperture of stomata (n = 20) in the center of the lower epidermis of the second fully expanded leaf from the top of four plants in each treatment were observed and imaged via a scanning electron microscopy at 2000 × magnification (Fig. 7).
The data were recorded and processed using Excel 2010 (Microsoft Inc., WA, Redmond, USA) and SPSS 19.0 software (SPSS Inc., Chicago, IL, USA). The variance and significance of the data were analysed. To detect significance differences between the means, Duncan's multiple range test was performed at a significance level of P < 0.0558.
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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This publication was supported by the project of Open Research Fund of China Meteorological Administration (stqx2018zd01) and the national science and technology innovation project to increase grain yield and efficiency of China (2018YFD0300103-1).
The authors declare no competing interests.
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Sun, Y., Xu, J., Miao, X. et al. Effects of exogenous silicon on maize seed germination and seedling growth. Sci Rep 11, 1014 (2021). https://doi.org/10.1038/s41598-020-79723-y
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