Abstract
Crystallization is the bottleneck in macromolecular crystallography; even when a protein crystallises, crystal packing often influences ligand-binding and protein–protein interaction interfaces, which are the key points of interest for functional and drug discovery studies. The human hypoxia-inducible factor prolyl hydroxylase 2 (PHD2) readily crystallises as a homotrimer, but with a sterically blocked active site. We explored strategies aimed at altering PHD2 crystal packing by protein modification and molecules that bind at its active site and elsewhere. Following the observation that, despite weak inhibition/binding in solution, succinamic acid derivatives readily enable PHD2 crystallization, we explored methods to induce crystallization without active site binding. Cyclic peptides obtained via mRNA display bind PHD2 tightly away from the active site. They efficiently enable PHD2 crystallization in different forms, both with/without substrates, apparently by promoting oligomerization involving binding to the C-terminal region. Although our work involves a specific case study, together with those of others, the results suggest that mRNA display-derived cyclic peptides may be useful in challenging protein crystallization cases.
Similar content being viewed by others
Introduction
X-ray diffraction analysis of proteins and their complexes is a mainstay of modern biological sciences and medicinal chemistry, yet protein crystallization, in particular of forms reflecting the solution state, is often a stumbling block in biophysical studies. Multiple strategies have been explored to stabilise proteins/protein complexes and/or to reduce the protein conformational heterogeneity, which in general hinders crystallization. Such strategies include, but are not limited to, protein construct design1, reduction of surface entropy2, co-complexation with natural/therapeutic ligands/chemical probes3 and binding of auxiliary proteins such as antibody fragments or alternative scaffolds4,5,6,7. Here we describe our experience in crystallizing a challenging human protein target and how this led us to a non-standard strategy to obtain different crystal forms. To obtain robust crystallization conditions for hypoxia-inducible factor prolyl hydroxylase 2 (PHD2) in complex with inhibitors and substrates, we explored all of the aforementioned approaches, but none has been efficient to date. As an alternative, we explored the use of cyclic peptides (CPs), which we identified via application of mRNA display technology8,9,10; this led to a CP that binds tightly to the catalytic domain of PHD2 (KD value of 270 pM)8 at a site away from the active site in a manner that efficiently enables efficient crystallization in previously unobserved forms.
In animals, the α,β-heterodimeric hypoxia-inducible factors (HIFs) activate an array of genes including those encoding for vascular endothelial growth factor (VEGF) and erythropoietin (EPO), which work to ameliorate the effects of hypoxia11,12. Under normoxic conditions, HIFα subunits are rapidly destroyed via ubiquitination involving an E3 ligase complex (von Hippel Lindau protein, Elongins B and C, Cul2 and Rbx1) and proteasomes13,14. The degradation of HIFα subunits is promoted by post-translational hydroxylation of prolyl-residues located in its N/C-terminal oxygen dependent degradation domains (NODD and CODD)15,16. These dioxygen-sensitive reactions are catalysed by 2-oxoglutarate (2OG) and Fe(II) dependent prolyl hydroxylases (PHD 1–3 in humans)11,12,17. Pharmacological manipulation of the hypoxic response via manipulating PHD activity offers the possibility of treating tumours and ischemia related diseases. Small-molecule PHD inhibitors are approved/in clinical trials for the treatment of anaemia associated with chronic kidney diseases18,19, which is presently treated with erythropoietin (EPO), a common medicine for anaemia20.
The development of clinically useful PHD inhibitors has been enabled by structural studies. Crystal structures of the PHD2 catalytic domain (aa 181–426) were initially reported in complex with a transition metal ion and bicyclic inhibitors (which are related to FG2216, a PHD inhibitor that entered clinical trials) in the P63 crystal form21,22. Subsequently PHD2-ligand complex structures, including with 2OG (PDB: 3OUJ), have been reported in different space groups23,24. The structural work to date has employed PHD2 constructs truncated at the C-terminus, i.e. aa 181–392 which crystallises in the P212121 space group (PDB: 3OUI)24, aa 189–399 in P63 (PDB: 4JZR)25, aa 181–416 in both P63 (PDB: 3OUH)24 and P41 (PDB: 3OUJ)24, aa 184–419 (PDB: 4KBZ) and aa 181–416 (PDB: 5V18)23 in P41 forms. Collectively, these observations imply that interactions involving the C-terminal region of PHD2 and binding of specific ligands to the active site can have profound roles in enabling crystallization/different crystal packing of PHD2.
Although PHD2 is predominantly monomeric in solution, crystallographic analyses in the P63 form reveal homotrimeric organization with intermolecular interactions between the residues from C-terminal helix α4 of one monomer and the surrounding active site residues from the flexible β2/β3 loop of a neighbouring subunit21,22 (Fig. 1). This head-to-tail trimeric arrangement21,22 prevents the substrate from productively approaching the active site in the P63 crystal form. Disrupting the head-to-tail arrangement by engineering C-terminal residues that are directly involved in crystal packing enables crystallization of PHD2-ODD enzyme–substrate complexes (space group: P212121)26,27. However, this procedure has two limitations: compared to the P63 form, only very few crystals were obtained via removing such ‘unwanted’ oligomerization, thereby limiting investigations, such as analysing catalytic intermediates using time-resolved crystallography. Further, this crystallization process takes an extended period (months) during which time crystallization drops can become contaminated (e.g. by Gram-negative Stenotrophomonas spp., sometimes leading to orthorhombic crystals of Stenotrophomonas alkaline phosphatase28).
With the aim of developing new PHD2 crystal forms that may enable a ligand accessible active site, i.e. one free from crystal packing/conformational restraints, that is amenable to ligand and/or substrate binding, we explored diverse strategies to promote crystallization, including variation of active site ligands, manipulating PHD2 surface solvation/interactions by lysine-Nε-methylation29, and a non-standard method using non active site binding CPs. Here we report a methodology for the efficient crystallization of PHD2 complexes, with and without HIF-α ODD substrate present, by using tight binding reagents, i.e. CPs, which allow retention of catalytic activity, but which dramatically promote crystallization of PHD2 complexes.
Results
PHD2 crystallization can be induced by some ligands that bind weakly in solution
Following from the observation that specific heteroaromatic inhibitors with glycine side chains induce PHD2 crystallization in the P63 form21,22, we investigated the selectivity of small-molecule induced PHD2 crystallization. We employed 96-well screens for crystallizing PHD2 constructs (PHD2181-426 and PHD2181-407, hereafter nPHD2 and cPHD2, respectively) with a set of cyclic 2OG analogues and related small-molecules. We observed that crystallization of nPHD2 is induced by succinamic acid derivatives (SCAs), 4a, 4b, 7f. and 30a (Fig. S1). This is interesting because none of these SCAs are potent PHD2 inhibitors or stabilizers (as observed by MS, NMR and thermal shift analyses) in solution (Figs. S1, S2). Except for the nPHD2.4b complex, which crystallized in the P31 form, all the aforementioned SCAs crystallized with nPHD2 in the P63 form. The nPHD2.SCA complex structures manifest a similar ligand binding mode to that observed in the reported nPHD2.FG2216/P63 structures21,22, i.e. like FG2216 and related compounds, the SCAs coordinate the active site metal ion via their heteroaromatic ring nitrogen and side chain amide carbonyl oxygen (Fig. S1). However, while the bidentate coordination of the active site metal ion by SCAs forms an approximately planar six-membered chelate ring, more established PHD inhibitors, including FG2216, form a five-membered chelate ring (Fig. S1). The chelate ring size may affect the stability of the protein-complex30 and possibly its binding/inhibition potential. Although biophysical (MS, NMR) analyses suggest that SCAs form only a weak complex with monomeric nPHD2 (Fig. S2), the clear Fo-Fc difference density for the SCAs suggests that intermolecular interactions of SCAs notably with the C-terminus of a threefold symmetry related neighbour in the P63 form may both stabilize the ligand binding in the crystal lattice and help promote crystallization.
Surface methylation of PHD2
The results with small molecules prompted us to investigate other ways of inducing PHD2 crystallization. We modified the lysine residues (21) in the nPHD2 construct via reductive lysine-Nε-methylation, which has been used to change crystal packing contacts for crystallization of other proteins2,29 but not iron oxygenases such as the PHDs. Electrospray ionization mass spectrometry (ESI–MS) under denaturing conditions of the methylation product (nPHD2-Me) revealed masses corresponding to multiple dimethylation events, apparently of all 21 lysines plus at the N-terminal amine. We confirmed the methylation states by mass spectrometric (MS) analysis following trypsinolysis of nPHD2-Me; the so obtained LC–MS spectra differ from those of unmodified PHD2 implying ‘missed cleavages’. LC–MS/MS analyses on the fragments of nPHD2-Me provided MS/MS evidence for N-dimethylation of lysines (data not shown).
nPHD2-Me (rather unexpectedly) crystallized in a hexagonal rod morphology in complex with an SCA, 4a. Despite modifications of all 21 lysines in nPHD2, the structure was solved in the same form, i.e. the P63 form, as that of unmodified PHD2 with 4a/related ligands. Comparison of the nPHD2 and nPHD2-Me structures reveals very similar overall folds, with the β2/β3 ‘finger’ loop (that is involved in HIF ODD binding26,27) in an ‘open’ (i.e. non-productive) conformation in both cases (Fig. S3)21,27. Although lysine methylation did not alter the packing of nPHD2 crystals, some of the lysines including K216, K262, K291, K350, K400 and K402 became more ordered upon methylation as apparent in their difference (Fo-Fc) density maps (Fig. S3).
Because some of the Nε-methylated lysines in nPHD2-Me are involved in HIF-α ODD binding26,27, we tested the activity of nPHD2-Me by monitoring HIF-1α ODD hydroxylation and 2OG turnover (Fig. S3). Despite some evidence for modulation of 2OG turnover, the results show that under the tested conditions, nPHD2-Me has substantial catalytic activity with both CODD or NODD (Fig. S3). Overall, these results imply that the differences in surface chemistry induced by Nε-methylation of lysines are insufficient to enable the desired changes in crystallization.
Crystallization of PHD2 with cyclic peptides (CPs)
Together with previous observations22,24,25, the results presented here support the proposal that nPHD2 often preferentially crystallizes in the P63 form, independent of different types of chemical modifications or crystallization conditions. We therefore set out to investigate the crystallization potential of PHD2 surface-binders that form contacts in the crystalline lattice, but which do not cause loss of catalytic activity.
With this objective in mind, we investigated peptides binding to nPHD2, which were identified using the mRNA display based Random nonstandard Peptide Integrated Discovery (RaPID) method that has been used to identify both inhibitory and allosteric CPs, including ones selective for enzymes involved in signalling pathways and transcriptional regulation8,31,32. In the RaPID method, acyclic peptides are cyclized by SN2 reaction of a C-terminal cysteine and a N-terminal chloroacetyl group to give a thioether9. As described previously, we identified peptides binding tightly to PHD2 as revealed by NMR and other biophysical analyses, but which do not affect Fe(II)/2OG/substrate binding and which allow efficient catalysis (we term these ‘non-competitive CPs’, NCCPs)8. Previously reported crystal structures of RaPID derived CPs in complex with KDM4A (a 2OG-oxygenase involved in epigenetic regulation)31 and Semaphorin 4D Receptor Plexin B1 (a signalling protein)33 reveal that in both cases, the target-selective CPs bind at the active site and adopt a distorted β-sheet fold with β-turns at the ends of the CP. Because the edges of β-sheets are intrinsically prone to undergo β H-bonding with other β-strands, as manifested in fibrils, and β-rich proteins34,35, we reasoned that the NCCPs, which can be readily prepared by routine solid phase peptide synthesis, might enable ordered oligomerization leading to crystallization of PHD2.
We successfully crystallized cPHD2 in the presence of a 14-residue NCCP (3C), obtained in a RaPID with five rounds of screening8. Multiple crystals were obtained in the presence of 3C and active site binding inhibitors, such as NOG (N-oxalylglycine, a close 2OG analogue) and FG2216, in the hexagonal P65 crystal form (Figs. 2 and 3). Pleasingly, we also discovered that 3C enables rapid crystallization of cPHD2 in the presence of 2OG (or NOG) and the substrate, HIF-1α CODD (aa 556–574), in the P21212 form (Figs. 4 and 5). The addition of 3C had a dramatic effect on the efficiency of PHD2.substrate complex crystallization; we obtained a large number of cPHD2.3C crystals in complexes with substrate peptides that grew to full size within less than a week, compared to the substantially fewer crystals in > 6 months without 3C27.
Consistent with the reported NMR studies8, we obtained multiple crystal structures revealing that 3C binds in the region of cPHD2 immediately to the N-terminal side of the core distorted double stranded β-helix (DSBH) fold of PHD2 (Fig. 2). Interestingly, within the crystal lattice, 3C ‘slots’ into a tight groove between the non-DSBH β1 of one cPHD2 molecule and the C-terminal α4 (that is also involved in the P63 crystal packing) of a neighbouring symmetry related cPHD2 molecule (Fig. 2). Binding of 3C at the interface of two symmetry related PHD2 monomers likely promotes contacts productive for crystallization in part via projecting part of the C-terminal helix α4 (aa 400–404) away from the active site, in a manner that maintains interactions with substrates that are required for catalysis (Figs. 4 and 5). The C-terminal residues 400–404, which form part of the helix α4 in most PHD2 crystal structures without 3C22,26,27, are positioned to make anti-parallel H-bonds with 3C (aa 1–4) adopting a β-strand fold in all cPHD2.3C complexes (both with and without CODD) (Fig. 3). Except for the aforementioned C-terminal helix α4 residues 400–404, at least in the crystalline state, binding of 3C does not cause any substantial structural changes in the overall fold including of the core DSBH, consistent with the reported NMR studies on the cPHD2.3C complex (Fig. 4).
In the cPHD2.3C complex structures, 3C adopts a rectangular fold comprising two (almost) planar distorted β-strands connected by two type II beta turns with DY1, D6, W8 and T13 at its four corners (Fig. 3); most of the side chains do not protrude extensively (Fig. S4). To date, there is only a single reported structure of a 2OG-dependent oxygenase, i.e. the JmjC histone demethylase KDM4A in complex with a CP, where the cyclic peptide binds at the substrate interacting site and is inhibitory31. By comparison with the KDM4A.CP complex structure, 3C forms fewer intramolecular, but substantially more intermolecular interactions with cPHD2 (Fig. S5). 3C forms extensive backbone-to-backbone interactions with cPHD2 β1 (aa 204–214) and three residues located at the N-terminus (187–189) within the same cPHD2 monomer and six residues (399–404) located at the C-terminus of a symmetry related cPHD2 molecule (Fig. 3 and Fig. S5). In addition, there are backbone to side-chain polar interactions between L188PHD2 and F213PHD2 with R123C, and A399PHD2 with T53C, as well as side-chain to side-chain electrostatic/H-bond interactions of D212PHD2 with T133C and S113C, and of K186PHD2 with YD13C (Fig. 3). Although the 3C interacting PHD2 residues are well-conserved in all three human PHDs, they are different in other 2OG-oxygenases including the ribosomal prolyl hydroxylase (OGFOD1, Z = 17.4)36, nucleic acid demethylases (e.g., FTO, Z = 14.4)37, phytanoyl-CoA dioxygenase (PAHX, Z = 13.3)38 and factor inhibiting HIF (FIH, Z = 10.8)39, providing a structural basis for the high selectivity of 3C and related NCCPs for the PHDs (Fig. 3)8.
PHD catalysis involves coordinated movements, including of two flexible regions comprising a dynamic β2/β3 loop and the C-terminal helix region (including α4) (Fig. 4) that are directly involved in substrate binding26,27,40. Although binding of 3C induces structural changes in cPHD2 C-terminal residues (400–404) that form part of the C-terminal helix (α4), 3C binding allows protein–protein interactions between the cPHD2 C-terminus and the HIF-α substrate, consistent with the catalytic activity observed in the presence of 3C (Fig. 5). Comparison of HIF-α ODD substrate complex structures, i.e. nPHD2.2OG.CODD (PDB : 5L9B)26 and cPHD2.2OG.CODD.3C, reveals that 3C does not cause any substantial conformational changes in the β2/β3 loop which folds to enclose the substrate (Fig. 4), nor in any of the identified (by crystallography or NMR26,27) substrate binding or active site regions, an observation consistent with our biochemical observations8.
Discussion
We initially employed extensive screening of crystallization conditions to obtain PHD2 crystals. This work led us and others21,22,23,24 to the finding that nPHD2 readily crystalizes in the presence of particular heteroaromatic inhibitors, the side chain of which occupies the 2OG co-substrate binding site (the ‘P63’ crystal form). The P63 form is, however, not amenable to different types of ligand/substrate complex crystallization. With the aim of altering PHD2 crystal packing to enable efficient and robust generation of enzyme-ligand/substrate complexes, we screened for different types of active site binding ligands and employed surface methylation of lysine-residues.
Although reductive Nε-methylation of nPHD2 lysines did not enable us to obtain a different crystal packing other than the P63 form, Nε-methylation appears to improve the structural order/conformational stability for some of the flexible regions of nPHD2, suggesting it may be useful in crystallizing other oxygenases, including full length PHD constructs. In general, reductive alkylations of proteins are more likely complete or successful when target lysines are solvent exposed with relatively high accessible surface areas (ASA)2,29, as was the case with nPHD2.
Unexpectedly, the work with small molecule ligands led to the observation that the ability to induce formation of the homotrimeic crystal form does not correlate with either their active site ligand binding efficiency or inhibitory potency. This observation is notable because many structural biology approaches involving crystallography are aimed at identifying and exploiting tight binding ligands for the isolated macromolecule3. It is presently impractical to exhaustively screen very large numbers of combinations of crystallization conditions and weakly binding ligands; however, employing focused relevant compound sets, e.g. of diverse 2OG analogues in the case of 2OG oxygenases, and a sparse matrix method using ligand grid screens as we employed in this work may have wider applications. Crystallization of the nPHD2 in complex with weakly binding succinimide derivatives (SCAs) occurs under the physicochemical conditions established for nPHD2.FG2216 type inhibitor crystallization21,22. Compounds from both the FG2216 and SCA series enable nPHD2 crystallization with approximately equal efficiency, yet FG2216 (and related compounds) are significantly more potent inhibitors than the SCAs under the tested assay conditions. Nonetheless, the variable degrees of inhibition of different 2OG-oxygenases by the SCAs and related compounds (Fig. S1) opens up new possibilities for selective inhibitor design, e.g. by using knowledge of the active site, it may be possible to improve binding of the SCA series and induce PHD2 oligomerization as a means of inhibition.
The above procedures, i.e. use of orthosterically binding ligands/reduction of surface entropy, did not lead to desirable new PHD2 crystal forms, leading us to explore unconventional methods for crystallization. Following screening for cyclic peptides (CPs) binding to PHD2 via a modified mRNA display methodology (Fig. 6), we identified a 14-mer cyclic peptide thioether (3C) that promotes crystallization of the catalytic domain of PHD2, whilst still enabling a catalytically productive substrate binding mode. 3C is a powerful tool, because it enables efficient crystallization of PHD2 in complexes with PHD inhibitors, including those that are in clinical trials, but potentially also for conducting more detailed structural analyses of catalytic intermediates, e.g. by time-resolved crystallography. Given the efficiency and cost-effectiveness of the RaPID methodology (e.g. compared to classical high throughput small-molecule screening) and the availability of peptides on scales suitable for biophysical analyses via solid phase synthesis, it is possible that the method will have general applicability in crystallization (Fig. 6).
In our case, the method yielded an NCCP that does not interfere with substrate binding; this is likely in part because the active site pocket of PHD2 can be obscured by dynamic loop conformations involved in induced fit during catalysis26, thus biasing the RaPID screen to identifying peptides binding elsewhere. In some cases, it may be productive to protect or block an active site/pocket (e.g. by a tight binding ligand) or other potential interaction sites (e.g. protein-nucleic acid/protein–protein interaction motifs) during the mRNA display screening process in order to identify surface binding NCCPs for promoting crystallization.
The human PHDs catalyse hydroxylation of prolyl-residues within the N/C terminal ODDs of HIF-1/2/3α and are negative regulators of the transcriptionally regulated hypoxic response11,12. In addition, there are reports that the PHDs catalyse prolyl hydroxylation of non-HIF-α substrates in cells41, though these reports need to be validated42. Except for HIF-1α NODD and CODD26,27, there is no structural information available on how PHDs catalyse hydroxylation of different HIF isoforms or non-HIF substrates and how they achieve selectivity in cells. Our work demonstrates the potential for NCCPs for structural analyses of PHD complexes, not only with inhibitors, but with different HIF ODDs/potential non-HIF substrates, which have otherwise been difficult to achieve.
The longstanding challenge of efficiently crystallising proteins has motivated efforts to develop innovative methods that induce ordered oligomerization. Use of highly soluble proteins as fusions has enabled determination of structures of many classes of proteins. For example, use of T4 lysozyme to replace part of the third intracellular loops or the N-termini of G protein-coupled receptors (GPCRs) has enabled determination of the structures of several GPCR complexes4,43,44. However, in many cases (including GPCRs), the production of such fusion proteins often leads to loss of activity or a failure to yield well diffracting crystals45. There are also many examples of using small molecule additives and auxiliary proteins, sometimes termed ‘crystallization chaperones’, including antibodies, nanobodies/FAB fragments, to aid crystallization5,46,47. Nanobodies have elicited interest in chaperoning protein crystallization due to their ability to reduce conformational heterogeneity and shield unproductive surfaces from solvents, whilst extending crystallographically productive surfaces to form crystal contacts4,47. However, generating antibodies/nanobodies and characterizing their complexes with protein of interest is time-consuming and can lead to structures that are not biologically representative46,47.
Although our results involve a specific case, together with other studies on CPs9,10, they suggest that use of readily synthesised non-competitive peptides (NCCPs) that can bind at the intermolecular interfaces between proteins and induce crystallogenesis are worthy of further investigation as a more general method to aid in protein/macromolecular crystallization. Although there is effort in initially setting up the RaPID method (Fig. 6), once established, it is robust, cost-effective (especially compared to operation of large small molecule libraries) and is easy to operate9. CP assisted crystallization has the potential advantage over the use of nanobodies or other recombinant crystallization ‘chaperones’ that the CPs are relatively small, being typically < 20 residues, compared to a single immunoglobulin domain of ∼125 residues. The use of CPs is thus likely to increase the chance of preserving native folds compared to crystallization chaperones. Compared to some antibody based methods, CP generation does not need for animal immunization/hybridoma technology. Once identified, the CPs can be easily synthesized or purchased, without requiring any specialized expression system. By contrast with proteins prepared by translation, since the CPs are prepared by synthesis, non-proteinogenic/unnatural residues can also be readily incorporated into them. CPs thus are stable, low mass, cost-effective, and tight binding molecules (KD values in the range of nM to pM)9, which are suited for crystallization screening in a wide range of physicochemical conditions.
Materials and methods
Recombinant PHD2 proteins were produced in E. coli and purified by metal affinity and size exclusion chromatography as reported26. The in vitro selection of CPs binding to biotinylated His6-PHD2 was carried out using RaPID methodology as reported8. Peptides were produced by standard Fmoc-solid phase peptide synthesis, all with an amidated C-terminus and a chloroacetylated N-terminus. Peptides were cleaved from the resin with a TFA-based cleavage mixture, cyclised to give a thioether link, then purified by HPLC as reported8. Assays comprised incubation with Fe(II)/2OG/substrate(s) followed by MS and/or NMR analyses as reported26. cPHD2.3C complex crystals were grown by vapour diffusion at 22 °C in 300 nL sitting drops with 2:1 or 1:1 or 1:2 ratio of sample (1.0 mM cPHD2, 1.5 mM MnCl2, 2.0 mM 2OG/NOG/FG2216, 1.0 mM 3C, with/without 2.0 mM CODD) to well solution (19–20% w/v polyethylene glycol 3350, 0.3 M magnesium formate, 2 mM MnCl2) and cryo-cooled in N2(l). Details of nPHD2.SCA complex crystallization are in Supplementary Methods. Data were collected at the Diamond Light Source (DLS) and the European Synchrotron Radiation Facility (ESRF) MX beamlines (Tables S1 and S2). The structures were solved by molecular replacement using PDB ID: 5L9B, 4BQX, and 5L9R as initial models. See Supplementary Methods for details.
Statistical analysis
Endpoint assay results are the mean of three independent experiments with error bars representing the s.e.m. For kinetic measurements, each experiment was carried out (at least) in triplicate (n = 3–5).
Data availability
GenBank accession codes for the sequences mentioned in this article are as follows: Q9GZT9 (EGLN1_HUMAN); Q16665 (HIF1A_HUMAN). Atomic coordinates and structure factors for the crystal structures of nPHD2.4a (PDB: 6YVX), nPHD2.7f. (PDB: 6YVW), nPHD2.30a (PDB: 6YVZ), nPHD2-Me.4a (PDB: 6YW0), cPHD2.2OG.3C (PDB: 6YW1), cPHD2.FG2216.3C (PDB: 6YW2), cPHD2.NOG.3C (PDB: 6YW4) and cPHD2.NOG.3C.CODD (PDB: 6YW3), and are deposited in the protein databank (wwPDB) and will be released on acceptance. Additional data supporting the findings of this study are available from the corresponding authors on request.
References
Derewenda, Z. S. The use of recombinant methods and molecular engineering in protein crystallization. Methods 34, 354–363. https://doi.org/10.1016/j.ymeth.2004.03.024 (2004).
Derewenda, Z. S. & Vekilov, P. G. Entropy and surface engineering in protein crystallization. Acta Crystallogr. D Biol. Crystallogr. 62, 116–124 (2006).
Vedadi, M. et al. Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination. Proc. Natl. Acad. Sci. USA 103, 15835–15840. https://doi.org/10.1073/pnas.0605224103 (2006).
Bukowska, M. A. & Grutter, M. G. New concepts and aids to facilitate crystallization. Curr. Opin. Struct. Biol. 23, 409–416. https://doi.org/10.1016/j.sbi.2013.03.003 (2013).
Koide, S. Engineering of recombinant crystallization chaperones. Curr. Opin. Struct. Biol. 19, 449–457. https://doi.org/10.1016/j.sbi.2009.04.008 (2009).
King, N. P. et al. Accurate design of co-assembling multi-component protein nanomaterials. Nature 510, 103–108 (2014).
Yeates, T. O. Geometric principles for designing highly symmetric self-assembling protein nanomaterials. Annu. Rev. Biophys. 46, 23–42 (2017).
McAllister, T. E. et al. Non-competitive cyclic peptides for targeting enzyme-substrate complexes. Chem. Sci. 9, 4569–4578. https://doi.org/10.1039/c8sc00286j (2018).
Tsiamantas, C., Otero-Ramirez, M. E. & Suga, H. Discovery of functional macrocyclic peptides by means of the RaPID system. Methods Mol. Biol. 299–315, 2019. https://doi.org/10.1007/978-1-4939-9504-2_14 (2001).
Malde, A. K., Hill, T. A., Iyer, A. & Fairlie, D. P. Crystal structures of protein-bound cyclic peptides. Chem. Rev. 119, 9861–9914. https://doi.org/10.1021/acs.chemrev.8b00807 (2019).
Chowdhury, R., Hardy, A. & Schofield, C. J. The human oxygen sensing machinery and its manipulation. Chem. Soc. Rev. 37, 1308–1319 (2008).
Schofield, C. J. & Ratcliffe, P. J. Oxygen sensing by HIF hydroxylases. Nat. Rev. Mol. Cell. Biol. 5, 343–354 (2004).
Ivan, M. et al. HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing. Science 292, 464–468 (2001).
Jaakkola, P. et al. Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation. Science 292, 468–472 (2001).
Epstein, A. C. et al. C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation. Cell 107, 43–54 (2001).
Masson, N., Willam, C., Maxwell, P. H., Pugh, C. W. & Ratcliffe, P. J. Independent function of two destruction domains in hypoxia-inducible factor-alpha chains activated by prolyl hydroxylation. Embo J. 20, 5197–5206 (2001).
Kaelin, W. G. Jr. & Ratcliffe, P. J. Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway. Mol. Cell 30, 393–402 (2008).
Maxwell, P. H. & Eckardt, K. U. HIF prolyl hydroxylase inhibitors for the treatment of renal anaemia and beyond. Nat. Rev. Nephrol. https://doi.org/10.1038/nrneph.2015.193 (2015).
Yan, L., Colandrea, V. J. & Hale, J. J. Prolyl hydroxylase domain-containing protein inhibitors as stabilizers of hypoxia-inducible factor: small molecule-based therapeutics for anemia. Expert Opin. Ther. Pat. 20, 1219–1245. https://doi.org/10.1517/13543776.2010.510836 (2010).
Chan, M. C., Holt-Martyn, J. P., Schofield, C. J. & Ratcliffe, P. J. Pharmacological targeting of the HIF hydroxylases: a new field in medicine development. Mol. Aspects Med. 47–48, 54–75. https://doi.org/10.1016/j.mam.2016.01.001 (2016).
McDonough, M. A. et al. Cellular oxygen sensing: crystal structure of hypoxia-inducible factor prolyl hydroxylase (PHD2). Proc. Natl. Acad. Sci. U S A 103, 9814–9819 (2006).
Chowdhury, R. et al. Selective small molecule probes for the hypoxia inducible factor (HIF) prolyl hydroxylases. ACS Chem. Biol. 8, 1488–1496. https://doi.org/10.1021/cb400088q (2013).
Ahmed, S. et al. 1,2,4-Triazolo-[1,5-a]pyridine HIF prolylhydroxylase domain-1 (PHD-1) inhibitors with a novel monodentate binding interaction. J. Med. Chem. 60, 5663–5672. https://doi.org/10.1021/acs.jmedchem.7b00352 (2017).
Rosen, M. D. et al. Benzimidazole-2-pyrazole HIF prolyl 4-hydroxylase inhibitors as oral erythropoietin secretagogues. ACS Med. Chem. Lett. 1, 526–529. https://doi.org/10.1021/ml100198y (2010).
Deng, G. et al. Novel complex crystal structure of prolyl hydroxylase domain-containing protein 2 (PHD2): 2,8-Diazaspiro[4.5]decan-1-ones as potent, orally bioavailable PHD2 inhibitors. Bioorg. Med. Chem. 21, 6349–6358. https://doi.org/10.1016/j.bmc.2013.08.046 (2013).
Chowdhury, R. et al. Structural basis for oxygen degradation domain selectivity of the HIF prolyl hydroxylases. Nat. Commun. 7, 12673. https://doi.org/10.1038/ncomms12673 (2016).
Chowdhury, R. et al. Structural basis for binding of hypoxia-inducible factor to the oxygen-sensing prolyl hydroxylases. Structure 17, 981–989 (2009).
Keegan, R. et al. The 11 angstrom resolution structure of a periplasmic phosphate-binding protein from Stenotrophomonas maltophilia: a crystallization contaminant identified by molecular replacement using the entire Protein Data Bank. Acta Crystallogr. D 72, 933–943. https://doi.org/10.1107/S2059798316010433 (2016).
Walter, T. S. et al. Lysine methylation as a routine rescue strategy for protein crystallization. Structure 14, 1617–1622 (2006).
Merkel, M. et al. Chelate ring size variations and their effects on coordination chemistry and catechol dioxygenase reactivity of iron(III) complexes. Inorg. Chem. 44, 7582–7589 (2005).
Kawamura, A. et al. Highly selective inhibition of histone demethylases by de novo macrocyclic peptides. Nat. Commun. 8, 14773. https://doi.org/10.1038/ncomms14773 (2017).
Yamagata, K. et al. Structural basis for potent inhibition of SIRT2 deacetylase by a macrocyclic peptide inducing dynamic structural change. Structure 22, 345–352. https://doi.org/10.1016/j.str.2013.12.001 (2014).
Matsunaga, Y., Bashiruddin, N. K., Kitago, Y., Takagi, J. & Suga, H. Allosteric inhibition of a semaphorin 4D receptor plexin B1 by a high-affinity macrocyclic peptide. Cell. Chem. Biol. 23, 1341–1350. https://doi.org/10.1016/j.chembiol.2016.09.015 (2016).
Richardson, J. S. & Richardson, D. C. Natural beta-sheet proteins use negative design to avoid edge-to-edge aggregation. Proc. Natl. Acad. Sci. USA 99, 2754–2759. https://doi.org/10.1073/pnas.052706099 (2002).
Makabe, K. & Koide, S. The promiscuity of beta-strand pairing allows for rational design of beta-sheet face inversion. J. Am. Chem. Soc. 130, 14370–14371. https://doi.org/10.1021/ja805011h (2008).
Horita, S. et al. Structure of the ribosomal oxygenase OGFOD1 provides insights into the regio- and stereoselectivity of prolyl hydroxylases. Structure 23, 639–652. https://doi.org/10.1016/j.str.2015.01.014 (2015).
Han, Z. et al. Crystal structure of the FTO protein reveals basis for its substrate specificity. Nature 464, 1205–1209. https://doi.org/10.1038/nature08921 (2010).
McDonough, M. A. et al. Structure of human phytanoyl-CoA 2-hydroxylase identifies molecular mechanisms of Refsum disease. J. Biol. Chem. 280, 41101–41110. https://doi.org/10.1074/jbc.M507528200 (2005).
Elkins, J. M. et al. Structure of factor-inhibiting hypoxia-inducible factor (HIF) reveals mechanism of oxidative modification of HIF-1 alpha. J. Biol. Chem. 278, 1802–1806. https://doi.org/10.1074/jbc.C200644200 (2003).
Flashman, E. et al. Kinetic rationale for selectivity toward N- and C-terminal oxygen-dependent degradation domain substrates mediated by a loop region of hypoxia-inducible factor prolyl hydroxylases. J. Biol. Chem. 283, 3808–3815 (2008).
Wong, B. W., Kuchnio, A., Bruning, U. & Carmeliet, P. Emerging novel functions of the oxygen-sensing prolyl hydroxylase domain enzymes. Trends Biochem. Sci. 38, 3–11. https://doi.org/10.1016/j.tibs.2012.10.004 (2013).
Cockman, M. E. et al. Lack of activity of recombinant HIF prolyl hydroxylases (PHDs) on reported non-HIF substrates. Elife 8, 1. https://doi.org/10.7554/eLife.46490 (2019).
Rosenbaum, D. M. et al. GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function. Science 318, 1266–1273. https://doi.org/10.1126/science.1150609 (2007).
Chun, E. et al. Fusion partner toolchest for the stabilization and crystallization of G protein-coupled receptors. Structure 20, 967–976. https://doi.org/10.1016/j.str.2012.04.010 (2012).
Singh, A. K., McGoldrick, L. L., Saotome, K. & Sobolevsky, A. I. X-ray crystallography of TRP channels. Channels (Austin) 12, 137–152. https://doi.org/10.1080/19336950.2018.1457898 (2018).
Kim, J. W. et al. Application of antihelix antibodies in protein structure determination. Proc. Natl. Acad. Sci. USA 116, 17786–17791. https://doi.org/10.1073/pnas.1910080116 (2019).
Pardon, E. et al. A general protocol for the generation of Nanobodies for structural biology. Nat. Protoc. 9, 674–693. https://doi.org/10.1038/nprot.2014.039 (2014).
Acknowledgements
We thank the Wellcome Trust, Cancer Research UK, European Research Council Starting Grant (EPITOOLS-679479), Oxford British Heart Foundation Centre of Research Excellence (RE/13/1/30181), the Biotechnological and Biological Research Council, the Medical Research Council, and the Engineering and Physical Sciences Research Council for funding. We thank Jasmin Mecinović (University of Southern Denmark), Holgar Kramer (London Institute of Medical Sciences), Luke McNeill (Oxford Nanopore Technologies Ltd) and the scientists of Diamond Light Source (DLS) and the European Synchrotron Radiation Facility (ESRF) for technical assistance. We thank Hiroaki Suga (University of Tokyo) for kind encouragement and collaboration on the development of cyclic peptides binding to PHD2.
Author information
Authors and Affiliations
Contributions
R.C. cloned the constructs, purified proteins, performed protein chemistry, MS analyses and crystallography. M.I.A. carried out 1H-CPMG and wLOGSY NMR analyses. T.M. and B. Bhushan synthesised and characterised CPs with supervision from A.K. B.B. and JLS synthesised and characterized SCAs. C.J.S. and R.C. designed the studies and wrote the paper with help from others.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Chowdhury, R., Abboud, M.I., McAllister, T.E. et al. Use of cyclic peptides to induce crystallization: case study with prolyl hydroxylase domain 2. Sci Rep 10, 21964 (2020). https://doi.org/10.1038/s41598-020-76307-8
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-020-76307-8
This article is cited by
-
Deficiency in PHD2-mediated hydroxylation of HIF2α underlies Pacak-Zhuang syndrome
Communications Biology (2024)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.