Abstract
River deltas are frequently facing salinity intrusion, thus challenging agricultural production in these areas. One adaption strategy to increasing salinity is shrimp production, which however, heavily relies on antibiotic usage. This study was performed to evaluate the effect of increasing salinity on the dissipation rates of antibiotics in tropical flooded soil systems. For this purpose, paddy top soil from a coastal Vietnamese delta was spiked with selected frequently used antibiotics (sulfadiazine, sulfamethazine, sulfamethoxazole, trimethoprim) and incubated with flood water of different salt concentrations (0, 10, 20 g L−1). Antibiotic concentrations were monitored in water and soil phases over a period of 112 days using liquid chromatography and tandem mass spectrometry. We found that sulfamethazine was the most persistent antibiotic in the flooded soil system (DT50 = 77 days), followed by sulfadiazine (DT50 = 53 days), trimethoprim (DT50 = 3 days) and sulfamethoxazole (DT50 = 1 days). With the exception of sulfamethoxazole, the apparent distribution coefficient increased significantly (p < 0.05) for all antibiotics in course of the incubation, which indicates an accumulation of antibiotics in soil. On a whole system basis, including soil and water into the assessment, there was no overall salinity effect on the dissipation rates of antibiotics, suggesting that common e-fate models remain valid under varying salinity.
Similar content being viewed by others
Introduction
Salinity intrusion is a natural phenomenon in coastal ecosystems that becomes particularly relevant in delta regions1,2. However, synergistic effects with anthropogenic drivers like groundwater overuse, hydro-dam construction, and sea level rise currently exacerbate salinization of deltaic soil and freshwater resources and restrict freshwater related agriculture2,3. In the Mekong Delta of Vietnam, for instance, salinization has already become a major threat4. About 1.8 million ha of land are affected by increasing salinity5, and the El Niño dry season 2015–2016 damaged up to 240,000 ha of paddy rice fields6. One approach to adapt to this changing environment is by shifting the freshwater land use systems, like paddy rice, to brackish or saltwater compatible agriculture, such as shrimp production7. Intensive shrimp production, however, frequently goes along with heavy use of antibiotics8, which disseminate in the environment. Accordingly, in shrimp ponds, single substances have already been detected in concentrations of up to 0.82 g kg−1 in pond mud and 2.39 mg L−1 in pond water9, thus posing risks for the selection and spread of antibiotic resistances10.
The final fate of antibiotics likely depends on soil order and temperature11,12, as well as several physico-chemical soil properties affecting sorption rates and dissipation13,14. Several studies dealt with dissipation rates of antibiotics in soil under field (e.g.11,15) or laboratory conditions (e.g.16–18). While field studies reflect natural conditions best, laboratory studies allow to determine the influence of a single factor on dissipation, in our case salinity, as environmental conditions can be fully controlled11,19. Results of such controlled experiments thus allow to estimate potential impacts of such factors on dissipation rates under field conditions11,20. Respective studies, however have been performed mainly under freshwater conditions, such as studies on the fate of antibiotics in either soil18,20 or water phase21,22. Only very few studies investigated the fate of antibiotics in flooded soil systems or water–sediment systems17,23,24. Even fewer studies monitored antibiotics in saline environment25,26. Only for sulfonamides it has been reported that photodegradation in pond water was not affected by salinity while microbial degradation in river water and activated sludge was inhibited22,27,28. For trimethoprim it was reported that its adsorption was reduced under elevated salt concentrations of marine sediments and activated sludge of saline sewage27,29. However, we are not aware of studies that explicitly considered the impact of salinity on the fate of antibiotics in soil and flooded ecosystems as typically found in deltas.
Therefore, the aims of this study were: (i) to determine antibiotic dissipation rates in permanent flooded soil, and (ii) to investigate the effects of different salinity concentrations on the dissipation rates of antibiotics. For this purpose we conducted a microcosm experiment to quantify the effect of salt on antibiotic’s dissipation rates. The antibiotics sulfadiazine (SDZ), sulfamethazine (SMZ), sulfamethoxazole (SMX) and trimethoprim (TMP) were selected based on their use in Asian aquaculture (frequency of application and amount) derived by former studies24,30, and due to their relevance in human medicine as persistent antibiotics contributing to the selection and dissemination of resistance genes.
Materials and methods
Study site and soil
As soils from the coastal Mekong Delta were pre-contaminated with antibiotics31,32, we used soil from the coastal agricultural area of Nam Dinh province in the Red River Delta (Vietnam, 20° 15′ 39.28″ N, 106° 29′ 28.14″ E) for this study. When screening this soil for antibiotic residues prior to the experiment, none of our target antibiotics were detected. We thus deemed this soil as ideal for mechanistic studies on antibiotic dissipation in the lab. The texture of the soil was classified as silty clay loam (SiCL) according to33 with following composition: 3.7% (± 0.6) sand, 59.4% (± 1.9) silt and 38.5% (± 0.6) clay. The soil had a soil organic carbon (Corg) content of 18.5 g kg−1 determined according to34, a pH(H2O) of 7.95 determined after35, and cation exchange capacity of 10.8 cmol kg−1 (± 0.89) determined according to36. After the World reference base for soil resources37 the soil was classified as Fluvisol. After sampling, soil was air-dried and sieved to a grain size < 2 mm.
Dissipation experiment
The dissipation experiment was conducted according to OECD Guideline 30738. Soil samples of 10 g were placed in centrifuges glasses and flooded with 25 mL water of three salinity concentrations (0 g L−1, 10 g L−1 and 20 g L−1). Salinity levels (10 g L−1 and 20 g L−1) were chosen to represent the salinity range in which shrimps (e.g. white leg or black tiger shrimp) can be cultured7. After a pre-incubation, water-soil systems were spiked with an antibiotic stock solution to achieve a target concentration of 60 µg L−1, respectively. The target concentration was chosen according to the routine limits of determination for the used antibiotics, adjusted to monitoring requirements for a dissipation study of 112 days. Antibiotic solutions for spiking were prepared in deionized water (prepared via Millipore purification system). After spiking, centrifuges glasses were covered with perforated aluminum foil to ensure air exchange and incubated in the dark at 25 °C for 0, 1, 3, 7, 14, 28, 56, and 112 days. Water-soil systems were prepared in triplicates for each extraction day and salt concentration. To verify microbial activity during experiment duration, respiration rates were determined for the incubation days 0, 56 and 112. Serum bottles were prepared in accordance with the incubation experiment in four repetitions per salt treatment and with and without antibiotic treatment. Respiration rates were determined by measuring the carbon dioxide concentration, using gas chromatography and flame ionization detector (GC-FID) (SRI, Torrance, USA) at the incubation day and the following day. The microbial activity was neither affected by the antibiotic treatment nor by the salt treatment (see Supplementary Table S1 online).
Chemicals and reagents
All solvents used for extraction were of HPLC grade. Sand utilized for accelerated solvent extraction (ASE) and acids were proanalysis grade. The used water was purified with a Millipore water treatment system. Analytical standards of SDZ, SMZ, SMX and TMP as well as isotope-labeled standards of SMZ (SMZ-D4, purity ≥ 98%), SMX (ring-13C6, purity ≥ 98%), and TMP (methyl-13C3, purity ≥ 98%) were obtained from LGC Standards (Wesel, Germany).
Sample extraction and purification
Antibiotics were analyzed separately in water, corresponding to easily extractable fraction, and soil, corresponding to the residual fraction11,12,39. Antibiotics were extracted in each system according to12.
Soil samples were extracted via accelerated solvent extraction (ASE; Dionex 350) using a methanol: water solution (1:1, v/v; according to40) and a 50 mM phosphoric acid: acetonitrile solution (50:50, v/v; according to41). The solutions used for ASE were adjusted to the water content of the samples.
Clean-up of the soil extracts and the water samples were performed using solid phase extraction (SPE). The samples were acidified with hydrochloric acid to pH 2.4 and loaded on the anion exchange cartridge (Chromabond SB, Macherey–Nagel, Düren, Germany) and adsorbent cartridge (OASIS HLB, Waters, Milford, United States). The antibiotics were eluted from the cartridges with 5 mL methanol, 5 mL acetonitrile and 5 mL of acidified acetonitrile (0.1% hydrochloric acid). Subsequently, the samples were evaporated to 0.5 mL in a rotary evaporator and filled up with 1 mL of 50 mM phosphoric acid: acetonitrile (80:20, v/v). Extracts were than stored at − 20 °C until analysis via liquid chromatography coupled to tandem mass spectrometry (LC/MS–MS). Laboratory blanks were taken regularly with each batch of samples.
Antibiotic quantification
Antibiotic concentrations in extracts were analyzed using a Thermo Fisher system composed of a liquid chromatography coupled with TSQ Quantum Ultra tandem mass spectrometry (LC/MS–MS) (Thermo Fisher, Dreieich, Germany). The mass spectrometer was equipped with a heated electrospray ionization source (HESI), operating in positive mode. Antibiotics were separated by an XBridge C18 3.5 µm, 2.1 × 150 mm HPLC column with guard column Sentry 2.1 × 10 mm (Waters, Milford, MA, USA). The solvents used as mobile phases were methanol (A) and Millipore-water (B) both acidified with 0.1% formic acid. The routine limits of quantification (RLOQ) were 7 ng L−1 in water and 33 ng kg−1 in soil for SMZ, SMX and TMP. The RLOQ for SDZ were 33 ng L−1 in water and 165 ng kg−1 in soil (used mass to charge ratios are listed in Supplementary Table S2 online). The recovery of antibiotics from the water-soil systems were in the range of 48.8 to 100.3%.
Data evaluation
For antibiotic dissipation calculations purposes, not detectable concentrations were set to zero and concentrations below RLOQ were set to the corresponding RLOQ. A single first-order exponential decay model (Eq. 1) and a first-order double-exponential decay model (Eq. 2) were fitted to the data, using nonlinear regression, where t is the time, Ct the antibiotic concentration at time t, C0 the concentration at time zero, C1 and C2 the constants for initial concentration of antibiotics in a fast and slow pool, respectively with k1 and k2 as the dissipation rate constants (k1 > k2).
Prior to the fitting procedure the data was linearized by log-transformation in order to decide on the use of the single or double exponential-model42. The half-life DT50 (time required for 50% of the initial concentration to dissipate) was calculated using:
Measured concentrations were normalized to their respective initial concentration to achieve consistency within the dissipation curves. Dissipation models were forced to the measured initial concentrations of the antibiotic accounting for differences in recovery.
To characterize the partitioning behavior of the antibiotics, apparent distribution coefficients (Kapp) were calculated for each extraction day as done by11,39. The Kapp values were calculated at given sampling time using Eq. (4) (modified equation according to11).
For statistical analysis, IMP SPSS version 25.0 (IMB Corp, Armonk, New York, USA) and gnuplot version 5.2 were used. All applied statistical tests were run with p = 0.05, when not stated otherwise. The Shapiro–Wilk test was applied to test for normal distribution of the data. To find significant differences ANOVA or tow-sample t-test were performed and the Games-Howell test or R-E-G-W Q test were used for post-hoc tests. For not normally distributed data, the non-parametric tests Kruskal–Wallis H or Mann–Whitney U were used to identify differences between and within groups, respectively. Asymptotic standard errors were calculated to monitor differences between the three salinity concentrations within the model calculation. Overlapping asymptotic standard errors indicate that differences between the dissipation dynamics did not exist.
Results
Dissipation rates in flooded soil systems
The dissipation of antibiotics in flooded soil systems followed single first-order kinetics for all studied sulfonamides and first-order double-exponential decay for TMP (Fig. 1). Dissimilarity in dissipation kinetics for TMP are due to the higher sorption affinity of TMP to the soil phase, identifiable by the calculated Kapp values (Fig. 2). The coefficient of determination R2 (i.e. the “goodness-of-fit”) ranged between 0.84 (SDZ, SMZ) and 0.99 (TMP) (Table 1). Antibiotic concentrations decreased significantly for all antibiotics within 112 days (p < 0.01) in the order SMX (99.4%) > TMP (86.9%) > SDZ (78.9%) and > SMZ (61.9%) (Fig. 1). Corresponding dissipation half-lives (DT50) increased in the same direction and were 1 day for SMX and 3 days for TMP, while DT50 of SDZ (53 days) and SMZ (77 days), both being nearly 20 times higher than for SMX and TMP (Table 1).
The partitioning of antibiotics between the water and soil phase changed over the incubation period. The relative amounts of antibiotics occurring in soil increased with increasing contact time, which is illustrated by an increase in Kapp values. Immediately after spiking (day 0), Kapp values were comparatively highest for TMP (67.4 ± 2.87 L kg−1) followed by SMZ (3.1 ± 0.13 L kg−1), SDZ (1.4 ± 0.05 L kg−1) and SMX (1.2 ± 0.06 L kg−1) (Fig. 2). After 112 days, Kapp values had increased significantly for SDZ (13.2 ± 2.66 L kg−1; p < 0.05), SMZ (20.8 ± 1.90 L kg−1; p < 0.05), and TMP (276.9 ± 13.92 L kg−1; p < 0.01) (Fig. 2).
Effect of salinity concentrations on dissipation rates
According to their dissipation profiles, the studied sulfonamides can be divided into two groups: SDZ and SMZ exhibited similar DT50 values in both soil and water phase, differing to DT50 values obtained for SMX (Table 1). Thus, in the following we concentrate on illustrating only representative dissipation processes of SDZ and SMX for sulfonamide dissipation, and show dissipation data for SMZ in the Supplementary (Table S2 online). Due to significant increases of the Kapp values, we discuss water and soil phase separately.
The R2 for the fitting of the dissipation curves ranged between 0.75 (SMZ) and 1.00 (SMX) for the water phase, and from 0.73 (SMZ) to 1.00 (SMX) for the soil phase (Table 1). Sulfadiazine dissipated faster in water than in soil, regardless of the prevailing salt concentration. The DT50 values ranged from a minimum of 15 days in water to a maximum of 87 days in soil (Table 1). The dissipation rates in water differed between the salinity levels, with a slower dissipation in the system without salt and DT50 values following the order 0 g L−1 (DT50 = 20 days) > 10 g L−1 (DT50 = 17 days) > 20 g L−1 (DT50 = 15 days; Table 1, Fig. 3a). SDZ dissipation in soil did not differ between the three salinity concentrations and ranged from 69 to 87 days, respectively (Fig. 3b, Table 1). The range observed in soil was larger than the difference observed for the water phase, reflecting than on a whole treatment basis (soil + water) no statistical differences were found among salinity levels (Table 1).
In contrast to SDZ (and SMZ; see Supplementary Fig. S1 online), the concentration of SMX decreased rapidly within the first 14 days for all salinity concentrations in the water and soil phase (Fig. 4, Table 1). Differences in dissipation rates between the three salinity levels were identified for SMX in water and also in soil phase. But in contrast to SDZ, dissipation of SMX was slowest at the intermediate salt concentration of 10 g L−1 compared to the other salinity levels (Fig. 4, Table 1). These differences in dissipation dynamics were mainly due to non-overlapping asymptotic standard errors between the incubation days 3 and 7 (Fig. 4), and possibly caused by chance (i.e., type I error), because absolute differences in DT50 values were smaller than a day. Furthermore, there is no real logical explanation for delayed dissipation at intermediate salinity levels, only.
The dissipation of TMP in water and soil phase deviated from that of the sulfonamides. Within the first days after application a rapid decline in TMP concentration was observed for both compartments, followed by a decelerated concentration decline after 7 to 14 days (Fig. 5). As a result, a first-order double-exponential decay model was needed to describe the dissipation behavior of TMP. In water, dissipation rates differed between the three salinity levels: dissipation at 0 g L−1 salt (DT50 = 2 days) was nearly two times faster than in the systems with 10 g L−1 salt (DT50 = 4 days; Fig. 5a; Table 1). This effect could not be confirmed for soil phase, where TMP dissipation did not differ between the three salinity levels, with DT50 ranging between 2 and 4 days (Table 1, Fig. 5b). Summing both phases together did not sustain statistical differences of the dissipation rates between the three salinity levels (Table 1).
Discussion
The obtained DT50 value of SDZ in the flooded soil system (53 days Table 1) was higher than that reported of24 in a water–sediment system of a mesocosm study (DT50 = 32 days). The temperature during the mesocosm study did not exceed 30 °C in the water, while in our experiment the ambient temperature was 25 °C. Studies of43 as well as44 reported that sulfonamide dissipation and temperature were correlated, with slower dissipation rates at decreasing temperature. Beside this11, observed systematically faster antibiotic dissipation under field conditions compared to laboratory conditions, which could at least in parts be forecasted using temperature-dependent DT50 values.
For SMZ the DT50 in the flooded soil system was 77 days, which was also higher than derived from DT50 values estimated in soil solely (DT50 = 19–35 days20,45). A higher persistence of SMZ might be due to sequestration processes that shift extractable antibiotic residues into a residual soil fraction while reducing the bioavailable one prone to microbial degradation10. This suggestion finds support by Kapp values that reached largest values for SMZ of all target sulfonamides (Fig. 2). A large potential for sulfonamides to be sequestered in soil has already been reported for the structurally related compound SDZ11,46.
In contrast to SDZ and SMZ, the rapid dissipation of SMX (DT50 = 1 days) was in agreement with previous findings under non-flooded conditions:16 found a rapid dissipation of SMX in soil within the first three incubation days (DT50 = 2–7 days) and47 observed up to 80% SMX dissipation within the 20 days of the experiment in loamy soil.
Despite the physico-chemical properties (listed in Supplementary Table S3 online) of sulfonamides indicate a rather weak sorption (log Kow SDZ = − 0.09 and SMZ = 0.28) and high mobility and bioavailability in the soil16, it has been noted that both SDZ and SMZ may form non-extractable residues (NER), especially in samples with high soil organic carbon contents17,48. The elevated organic carbon concentration (18.5 g kg−1) of the experiment soil gives support to the hypotheses that similar processes likely took place here. However, to detect NER formation, it would have been required to work with radiolabeled organic compounds. For the used sulfonamides, microbial degradation plays the major role in the dissipation process16,28 while chemical processes like hydrolysis were negligible16,22,48.
Previously, a DT50 value for TMP of 8 days in a water–sediment system had been reported24, thus exceeding estimated DT50 values for TMP in our experiment (DT50 = 3 days; Table 1). Here, we have to recall that TMP dissipation rates were calculated using a first-order double-exponential decay model, which includes the risk that it underestimates the chemical´s persistence substantially49. Other researchers observed a bi-phasic dissipation only for hydrophobic pesticides50, assuming a slower second-phase dissipation process due to the higher affinity to soil sorption. Among the compounds studied here, TMP had the highest Kd values of all target antibiotic in this study and additionally, the pronounced increase in Kapp values supports the idea that larger fractions of TMP were adsorbed to soil than for the sulfonamides (Fig. 2, Supplementary Table S4 online). The dissipation of TMP is usually controlled by biotic factors16,47. Abiotic factors like photolysis or hydrolysis can again be excluded, as the experiment were conducted in the dark and TMP does not consists of any hydrolysable groups or substituted amino groups16.
With the exception of SMX, all antibiotics accumulated in the soil during the experiment as indicated by the significant increase of the Kapp values (SDZ, SMZ: p < 0.05; TMP: p < 0.01; Fig. 2). This is in line with39 and11 who observed increasing Kapp values for SDZ within an incubation period of 218 and 288 days, respectively. Increasing Kapp values were also reported in the context of an increase in the residual fraction, which was characteristic for sequestration processes11,46. Differences to SDZ and SMZ might be attributed to deviations in chemical structure. Although SMX, SZD and SMZ belong to the same antibiotic class sulfonamides show differences in the environmental fate. For instance51, reported that the sorption of sulfonamides can be largely affected by differences in functional moieties. Since SDZ and SMZ have more similarities in their chemical structure than with SMX, this might explain the deviations in the partitioning behavior between the used sulfonamides. Also TMP showed an increasing affinity to the soil, with Kapp values more than 50 times higher compared to the sulfonamides, even at the first day of incubation (Fig. 2).
The above-mentioned discussion refers to the combined dissipation of our target compounds from soil and water phase together. When separating these two for SDZ, the dissipation was faster in the water phase (DT50 = 15–20 days; Table 1) than in other systems that solely investigated the dissipation of SDZ in water (DT50 < 120 days28; see also23,24). The main reason is likely the provision of additional soil surfaces for bacterial growth16,23,28. In soil, SDZ was found to be more persistent (DT50 = 69–86 days) than in the water phase (Fig. 3b), likely because of the formation of sequestered SDZ residues39,46, which have been included into our fractionation scheme.
When solely considering the water phase, SZD dissipation rates increased under the impact of higher salt concentration with a difference of 4 days in DT50 values between the systems with 0 g L−1 and those with 20 g L−1 salt (Fig. 3a). The main dissipation processes for SDZ in this experiment is assumed to be microbial degradation and sequestration. The former, however, should be reduced rather than enhanced in the salt-affected systems27, due to higher osmolality outside the cell52,53,54. As the soil used for this study originated from a coastal area, we may not fully discount the possibility that some members of the microbial community had been pre-exposed to salinity and thus were capable to adapt to a certain extent to this salinity55. Although the currently use of the soil for freshwater paddy rice production is not really in favor for this assumption. However, salinity has been reported to decrease sorption of antibiotics, particularly of those that bind to the solid soil matrix via ionic interaction to negatively charged surfaces27,29. Tropical soils frequently exhibit anion exchange capacity56 and there is no reason to ignore the possibility that in a similar manner also the anionic speciation of SDZ at the ambient pH may compete with accompanying chlorides and sulfates for sorption sites. In either case, the majority of SDZ primary binds to solid matrix via hydrophobic interactions51,57. This fraction is likely not affected by salinity, and as a result the overall effect of salts on SDZ dissipation in the water phase was low and absent for the soil phase.
The salinity effect observed for SDZ dissipation was not detected for SMZ (see Supplementary Fig. S1 online). Additionally, differences in the dissipation dynamics between the three salt treatments were not continuous over the whole incubation period. Moreover, for the rapidly dissipating antibiotic SMX, salinity effects on reduced dissipation rates were observed in the systems treated with 10 g L−1 salt, only (Table 1). As already indicated in the results section, there is no reasonable explanation that only intermediate salt concentrations might affect dissipation rates, so that this finding might well be a so-called alpha error in the statistical comparison. When taking into consideration that effects on DT50 were smaller than a day, the finding is also not relevant. For TMP, differences between the salinity levels were also limited to water phase and not continuous (Fig. 5). But this differences were negligible, as dissipation was not affected on a whole system basis, due to TMP mainly occurred in soil phase of the system (see Kapp values, Fig. 2). The sorption of TMP to marine sediments was investigated by29 and they observed lower TMP sorption under the influence of salt. Indeed, we also found lower Kapp values for TMP in the salt treated variants (10 and 20 g L−1) compared to the non-salt treated variant (0 g L−1) (see Supplementary Table S4 online). However, this finding did not affect TMP dissipation rates in soil. Overall, we thus have to refute the hypothesis that salinity exerts significant impacts on the dissipation rates of antibiotics in flooded soils.
Conclusion
Within this study we investigated the dissipation of antibiotics in a permanently flooded tropical soil under different levels of salinity. Sulfonamide dissipation rates could be described via first-order exponential decay model, while TMP dissipation rates were calculated via first-order double-exponential decay model due to higher sorption affinity. SMZ and SDZ were the most persistent antibiotics, the fate of which being influenced by sequestration processes. Particularly for SDZ, SMZ, and TMP, there was an enhanced partitioning between soil and water phase over the incubation period with an increasing accrual of the antibiotics in flooded soil. However, an overall effect of salinity on the dissipation rates of antibiotics could not be ascertained. Hence, existing e-fate models from terrestrial environment remain valid and thus may continued to be used to forecast the fate of at least selected antibiotics also under conditions of salinity intrusion in such river deltas.
References
Sánchez-Arcilla, A., Jiménez, J. A., Valdemoro, H. I. & Gracia, V. Implications of climatic change on spanish mediterranean low-lying coasts: the ebro delta case. J. Coast. Res. 242, 306–316 (2008).
Renaud, F. G., Le, T. T. H., Lindener, C., Guong, V. T. & Sebesvari, Z. Resilience and shifts in agro-ecosystems facing increasing sea-level rise and salinity intrusion in Ben Tre Province, Mekong Delta. Clim. Change 133, 69–84 (2015).
White, E. & Kaplan, D. Restore or retreat? Saltwater intrusion and water management in coastal wetlands. Ecosyst. Health Sustain. 3, e01258 (2017).
Field, C. B. Managing the Risks of Extreme Events and Disasters to Advance Climate Change Adaptation. Special Report of the Intergovernmental Panel on Climate Change/Edited by Christopher B. Field (Cambridge University Press, Cambridge, 2012).
Mekong River Commission (MRC). Basin Development Plan scenarios assessment. Technical Note 8: Impacts of changes in salinity intrusion (2010).
Nguyen, Y. T. B., Kamoshita, A., van Dinh, T. H., Matsuda, H. & Kurokura, H. Salinity intrusion and rice production in Red River Delta under changing climate conditions. Paddy Water Environ. 15, 37–48 (2017).
Nhan, D. K., Phap, V. A., Phuc, T. H. & Trung, N. H. Rice Production Response and Technological Measures to Adapt to Salinity Intrusion in the Coastal Mekong Delta (CanTho University Press, Can Tho, 2012).
Cabello, F. C. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ. Microbiol. 8, 1137–1144 (2006).
Le, T. X. & Munekage, Y. Residues of selected antibiotics in water and mud from shrimp ponds in mangrove areas in Viet Nam. Mar. Pollut. Bull. 49, 922–929 (2004).
Jechalke, S., Heuer, H., Siemens, J., Amelung, W. & Smalla, K. Fate and effects of veterinary antibiotics in soil. Trends Microbiol. 22, 536–545 (2014).
Rosendahl, I. et al. Dissipation and sequestration of the veterinary antibiotic sulfadiazine and its metabolites under field conditions. Environ. Sci. Technol. 45, 5216–5222 (2011).
Dalkmann, P. et al. Accumulation of pharmaceuticals, Enterococcus, and resistance genes in soils irrigated with wastewater for zero to 100 years in central Mexico. PLoS ONE 7, e45397 (2012).
Kemper, N. Veterinary antibiotics in the aquatic and terrestrial environment. Ecol. Indic. 8, 1–13 (2008).
Jeon, D. S. et al. Reactions and behavior relevant to chemical and physical properties of various veterinary antibiotics in soil. J. Fac. Agric. Kyushu Univ. 59, 391–397 (2014).
Blackwell, P. A., Kay, P. & Boxall, A. B. A. The dissipation and transport of veterinary antibiotics in a sandy loam soil. Chemosphere 67, 292–299 (2007).
Liu, F. et al. Dissipation of sulfamethoxazole, trimethoprim and tylosin in a soil under aerobic and anoxic conditions. Environ. Chem. 7, 370 (2010).
Carstens, K. L., Gross, A. D., Moorman, T. B. & Coats, J. R. Sorption and photodegradation processes govern distribution and fate of sulfamethazine in freshwater-sediment microcosms. Environ. Sci. Technol. 47, 10877–10883 (2013).
Fang, H., Han, Y., Yin, Y., Pan, X. & Yu, Y. Variations in dissipation rate, microbial function and antibiotic resistance due to repeated introductions of manure containing sulfadiazine and chlortetracycline to soil. Chemosphere 96, 51–56 (2014).
Kreuzig, R. & Höltge, S. Investigations on the fate of sulfadiazine in manured soil: laboratory experiments and test plot studies. Environ. Toxicol. Chem. 24, 771–776 (2005).
Accinelli, C., Koskinen, W. C., Becker, J. M. & Sadowsky, M. J. Environmental fate of two sulfonamide antimicrobial agents in soil. J. Agric. Food Chem. 55, 2677–2682 (2007).
Hoang, T. T. T., Tu, L. T. C., Le, N. P., Dao, Q. P. & Trinh, P. H. Fate of fluoroquinolone antibiotics in Vietnamese coastal wetland ecosystem. Wetl. Ecol. Manag. 20, 399–408 (2012).
Adamek, E., Baran, W. & Sobczak, A. Assessment of the biodegradability of selected sulfa drugs in two polluted rivers in Poland: effects of seasonal variations, accidental contamination, turbidity and salinity. J. Hazard. Mater. 313, 147–158 (2016).
Radke, M., Lauwigi, C., Heinkele, G., Mürdter, T. E. & Letzel, M. Fate of the antibiotic sulfamethoxazole and its two major human metabolites in a water sediment test. Environ. Sci. Technol. 43, 3135–3141 (2009).
Nguyen, D. G. C. et al. Occurrence and dissipation of the antibiotics sulfamethoxazole, sulfadiazine, trimethoprim, and enrofloxacin in the Mekong Delta, Vietnam. PLoS ONE 10, e0131855 (2015).
Zhang, R. et al. Antibiotics in the offshore waters of the Bohai Sea and the Yellow Sea in China: occurrence, distribution and ecological risks. Environ. Pollut. 174, 71–77 (2013).
Chen, H. et al. Antibiotics in typical marine aquaculture farms surrounding Hailing Island, South China: occurrence, bioaccumulation and human dietary exposure. Mar. Pollut. Bull. 90, 181–187 (2015).
Li, B. & Zhang, T. Biodegradation and adsorption of antibiotics in the activated sludge process. Environ. Sci. Technol. 44, 3468–3473 (2010).
Lai, H.-T., Wang, T.-S. & Chou, C.-C. Implication of light sources and microbial activities on degradation of sulfonamides in water and sediment from a marine shrimp pond. Bioresour. Technol. 102, 5017–5023 (2011).
Li, J. & Zhang, H. Factors influencing adsorption and desorption of trimethoprim on marine sediments: mechanisms and kinetics. Environ. Sci. Pollut. Res. 24, 21929–21937 (2017).
Sarmah, A. K., Meyer, M. T. & Boxall, A. B. A. A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment. Chemosphere 65, 725–759 (2006).
Managaki, S., Murata, A., Takada, H., Tuyen, B. C. & Chiem, N. H. Distribution of macrolides, sulfonamides, and trimethoprim in tropical waters: ubiquitous occurrence of veterinary antibiotics in the Mekong Delta. Environ. Sci. Technol. 41, 8004–8010 (2007).
Shimizu, A. et al. Ubiquitous occurrence of sulfonamides in tropical Asian waters. Sci. Total Environ. 452–453, 108–115 (2013).
Jahn, R. Guidelines for Soil Description 4th edn. (Food and Agriculture Organization of the United Nations, Rome, 2006).
ISO 10694:1995. Soil Quality - Determination of Organic and Total Carbon After Dry Combustion (Elementary Analysis) (1995).
Sonmez, S., Buyuktas, D., Okturen, F. & Citak, S. Assessment of different soil to water ratios (1:1, 1:2.5, 1:5) in soil salinity studies. Geoderma 144, 361–369 (2008).
Klute, A. Methods of Soil Analysis. 2nd edn. (American Society of Agronomy, Madison, 1982–86).
Food and Agriculture Organization of the United Nations. World reference base for soil resources 2014. International soil classification system for naming soils and creating legends for soil maps (FAO, Rome, 2014).
OECD. Test No. 302B. Inherent Biodegradability: Zahn-Wellens/EVPA Test (OECD, 1992).
Förster, M. et al. Sequestration of manure-applied sulfadiazine residues in soils. Environ. Sci. Technol. 43, 1824–1830 (2009).
Golet, E. M., Strehler, A., Alder, A. C. & Giger, W. Determination of fluoroquinolone antibacterial agents in sewage sludge and sludge-treated soil using accelerated solvent extraction followed by solid-phase extraction. Anal. Chem. 74, 5455–5462 (2002).
Göbel, A. et al. Extraction and determination of sulfonamides, macrolides, and trimethoprim in sewage sludge. J. Chromatogr. A 1085, 179–189 (2005).
Weihermüller, L., Neuser, A., Herbst, M. & Vereecken, H. Problems associated to kinetic fitting of incubation data. Soil Biol. Biochem. 120, 260–271 (2018).
Srinivasan, P. & Sarmah, A. K. Dissipation of sulfamethoxazole in pasture soils as affected by soil and environmental factors. Sci. Total Environ. 479–480, 284–291 (2014).
Ingerslev, F. & Halling-Sørensen, B. Biodegradability properties of sulfonamides in activated sludge. Environ. Toxicol. Chem. 19, 2467–2473 (2000).
Pan, M. & Chu, L. M. Adsorption and degradation of five selected antibiotics in agricultural soil. Sci. Total Environ. 545–546, 48–56 (2016).
Schauss, K. et al. Analysis, fate and effects of the antibiotic sulfadiazine in soil ecosystems. Trends Anal. Chem. 28, 612–618 (2009).
Wu, Y., Williams, M., Smith, L., Chen, D. & Kookana, R. Dissipation of sulfamethoxazole and trimethoprim antibiotics from manure-amended soils. J. Environ. Sci. Health B 47, 240–249 (2012).
Yang, J.-F. et al. Degradation behavior of sulfadiazine in soils under different conditions. J. Environ. Sci. Health B 44, 241–248 (2009).
Beulke, S. & Brown, C. Evaluation of methods to derive pesticide degradation parameters for regulatory modelling. Biol. Fertil. Soils 33, 558–564 (2001).
Laabs, V., Amelung, W., Pinto, A., Altstaedt, A. & Zech, W. Leaching and degradation of corn and soybean pesticides in an Oxisol of the Brazilian Cerrados. Chemosphere 41, 1441–1449 (2000).
Figueroa-Diva, R. A., Vasudevan, D. & MacKay, A. A. Trends in soil sorption coefficients within common antimicrobial families. Chemosphere 79, 786–793 (2010).
Singh, B. K., Walker, A., Morgan, J. A. W. & Wright, D. J. Effects of soil pH on the biodegradation of chlorpyrifos and isolation of a chlorpyrifos-degrading bacterium. Appl. Environ. Microbiol. 69, 5198–5206 (2003).
Yun, E.-Y., Ro, H.-M., Lee, G.-T. & Choi, W.-J. Salinity effects on chlorpyrifos degradation and phosphorus fractionation in reclaimed coastal tideland soils. Geosci. J. 14, 371–378 (2010).
Yan, N., Marschner, P., Cao, W., Zuo, C. & Qin, W. Influence of salinity and water content on soil microorganisms. Int. Soil Water Conserv. Res. 3, 316–323 (2015).
Wichern, J., Wichern, F. & Joergensen, R. G. Impact of salinity on soil microbial communities and the decomposition of maize in acidic soils. Geoderma 137, 100–108 (2006).
Brady, N. C. & Weil, R. R. The Nature and Properties of Soils 12th edn. (Prentice Hall, London, 1999).
Wegst-Uhrich, S. R., Navarro, D. A., Zimmerman, L. & Aga, D. S. Assessing antibiotic sorption in soil: a literature review and new case studies on sulfonamides and macrolides. Chem. Cent. J. 8, 5 (2014).
Acknowledgements
This study was funded by the Federal Ministry of Education and Research, Germany (031A287B) through the project “Sustainable adaptation of coastal agro-ecosystems to increased salinity intrusion (DeltAdapt)” in Vietnam. We kindly acknowledge support from the team of the Faculty of Environment of the Vietnam National University of Agriculture for supporting the soil sampling and the team of Institute of Crop Science and Resource Conservation (INRES)-Division Soil Science and Soil Ecology for supporting the laboratory work. Open Access funding provided by Projekt DEAL.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Sentek, V., Braun, G., Braun, M. et al. Salinity-independent dissipation of antibiotics from flooded tropical soil: a microcosm study. Sci Rep 10, 14088 (2020). https://doi.org/10.1038/s41598-020-70943-w
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-020-70943-w
This article is cited by
-
Study on the occurrence forms of oxytetracycline and sulfamethoxazole in saline soil and their influencing factors
Journal of Soils and Sediments (2024)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.